Chy12G213330 (gene) Cucumber (hystrix) v1

Overview
NameChy12G213330
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioneukaryotic translation initiation factor 2 subunit beta
LocationchrH12: 11079527 .. 11086770 (-)
RNA-Seq ExpressionChy12G213330
SyntenyChy12G213330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAATCCATTTTTGGATCCTGGCTTCCACCTCTCCTTCTGTATTTTCTGTTACAACAAAGGTTAGAGATCGTTGCAATTTGTTTCCATCCATCTAGATAGAGTCCTTGTTCTTGGATGTGACGTTTTTGATAGGTTCAATGGGAAAGAATTTGATGGTCATAGAAAAGCCATGGGATGAGATCCTTGAACATGAGAATGGGTATGGTCAAAAGTATATTTTGGTACAATTGCCGTGAGTTTCTCTTGGAAACACAGTCAAACTTTGTAAGTTTGAGTATTCTCCTTTAGATAAGTGGTGGAAGAAGTATAAAGGCAAAGCACAAATATGGTTGCATTCATCGGTGAGCAGATTCTTCACGTGGGTTGATTTTTATTGCTAATCACGTGTCAACCACCCCAAATGTAATTTGTCCACTTGTATGATTTATTACTGCTTGCCAGTGTTTATATTTTAACTTGGTAAAATTAACCCTCCTATACATTTTTGCAGGAACAATGGCGGACGAAGTTCAGAATGATGTGAATGATCAGGTTGCAGATGTGAGTTTTTTTGTTTCTTATCTATTATCACCATGCACATATATTTTCATTCAAAATTTTCACATATTGTGGGTCTTCCCATTTTTGTTTTTTCCTTTTCAATTATTCATCTAAGGAAACCAAAATCTTTGAGTTGTAATTTGGCGGACCAAACACTTCTGTGTTTCAACACCCAGGGGGAAATTCATTTCTACATGTATAGCTGAAGGTGTATATTATAACCCAATGCTATGCTTATGGTTGCGTGGTCTTGATTAGCAAGCATTCTGGAAACCCAAGGAATAAATCATAACTCATGAGGTTTGTGTGGTCTTGTTTCTGTTGGGAGTTTGGTTTTCAGTTTTCTGGTCAAAGAACGATCCGTGCTATGATCGAGGAGTTTCTTATGCATCCGCCTTTCAGAGAGAAAGGCTGGTTTTTGTGGCTTGCAGGAGTGTGTGTTGTGGTTTAGGATATTTGGGGTGGAAGGAACAAGAGAGTTTTTAGAGGTTTTGACTTTAGGGGATTTGGTTCTTTGTGAGATTTCATGCTTCTCTTTGGGCTTTGGTTTCAAAGTTTTTTTGTAATTATTTTCTAGGTAACATTTTGTTGAGTTGGAACCCTTTCTTGTACTGGGCTTGTTTTTGGTGGGCTTTTTTTTGTATGCCTTTGTATTCTTTCATTGAATGAAAGTTGTCTAGTTTCATTAAAAAGAAAAAATCGTAACCCATGAGTTAACTTAAATGCTGTACTATTAGTTGTCTAGTCTTGTTAAGATTACAGAATCCGATCCAATTCTTGATGTATTTTCTATTGGATTTTTTACTGGTGCTAAGCATCAAAGCTGCACTTTTTCAGTGAAGTTGCTCAGGCTGGTATCTTACTGTGCCTCTTTATATGAACATTTAAGTAATTGTAATCTCGTGCAGTTTCTGAACCGTTGTTCAAGTGTGGGCATTGATGCAGTATCATGATGCATGTGTATGGTTTATTTCTTGCAGATTGCCCCCTTTGATCCAACTAAGAAGAAGAAGAAGAAGAAGGTTGTACTTCAGGATCCGACTGATGAGAGTGTTGACAAGTTAGCAGAGAAAACTGAAAGTTTGTCAGGTATGAAAAGTTGACTTTCTGTTCCATGTTGTTTTCATGATATTTTCATTTTGTTGTTGATAAGAGGATTGTTGATGGATTTTTTATTTAATCTTGTAGTTTCTGATGGTTTGGAGGCTACAACATTTTCTGGACTTAAAAAGAAGAAGAAAAAACCAGTATGTTCTTGCTTTTCTTTGTTCAAGCTCTGTTTCGTGACTGATTTAATTTGTTTAATTTTAGGTAGAGGCAAGCTCCTTGAATGAAGATGGTGCAGATGCTACGGAGGATTTGGAAGGTTAGGAATGGATATTTAAACTTTGCCATGCCTTACCCTTTTCCTTTTCATTTTCATACAATTACATTTACTTTCTACGTCATGTTAATAATTGAGACAAACGAAGGTGTATAGTTTTCCTAAGTGATATCTTTTGAAGTGTTGGGGACAGTAGATTTTGTCATCGTTATAGTCACTTGGGGCTTAAATGTGGGTTGCAAAGAATAGTTTTATATACTAGTGAAAATAGTTTTATAAACCATAATATAATAAAAAATTTAAAAAATTGAAGCTTTTGTAGGCACACATGGTTTGACTCTGGCCCCTCAAGTCAAATAATCGATTTTATACATACTTATATGGATAGTTATATAATTGTTTATCATAATTTTGTAATTGGTATTGCTTAATCTAAATATTCTATATCAATGTGTTTAAAATTATATTTATGTGTGTGAAAACACAATTTAGTCCCTTTATTTTCAAACTTTTGTTTCATAAGCCTTTAAACACTTTTAATTTTGTGTTTAATGGGTCAATGTGACTACTTGATTTGGGAACTTTCAATTTGTATCAAATTGTTCTTTCACATATTTTAAATTTTTAAAATTCTAAGTATACATATATTTTTATTTTATGTCTAATTGAACATTAAACTTCCAATGTTGTAACTAGTAGATGCAATAATTAAGCATCTAATAGGGGACCTACTAGACACAAAATTAGATTTTATGTCTAACAAAACGTGTGGTGGTTTATAAATATTTTGAATGTGTAACAGACCTTTCAATATCTTTAAGCATTAGACCCTGAATTGAGAATTTAGTAATCTATTAGACACTTGACTAAAATACAAATTTCAAAGTTTATTGGCTAAACTTATTTAACCTTATATGAATTTTTATCTCCTCTGACATAAAGTTCTTGATTCACCATGGTGGAGAAAAACAAGCACTTTTAACGTTCATTTTTACGACCACTGACCACATACACACAAAGCTAAAAATTTGTTTGCTAAACTTGTTCAACTTAATATGAATTTGTATGCTGTATCTCCTCTAATGCAAAGTTCTTGATTTGCCACAATGGAGAAACATGGTTATTTTAAACATTCGGTGACGTAGGCACAAATTTATAAGTTCATCAGCTAAACTTATTTAACTTCTTATGTAGTGTATCCCTTTTGACACTAATTTAAAAGTTTATTGGCTAGAATTGTTTAACCTTATACCTATAGCATCCCCTCTGACACAAAGTGCTGGATTTGCCATGGTGGAGAAAGATGAACACTTCTAGAGTATGCCGTTTAGAAACTTAAAATTTAAAAAGAAAATGAAAAAGGAAAACAAAGGTTTCACATGGCTCGACATCACCTCAAGCTCATTTCATAAAGAGGGTGACTTGCTTTAGAAGCACGACACTTAGGGTGTGCTTTTCTGAAAAGCCATACTACTTAAAGAACTGTCTAAGTAGCCTTGCATTATATGGAGTTTCTATTGAAAAAGAGTTGTGTATGTAATTTATGCGTTTTTTGTTCATCATCTCCTTTTCAACTGTAGCCATTTTTTCAACTGAAAAGTGATCATCATGCCTATCAGATCAAGCGGGGGAAAATGAAGATCGTGAGGGACTTGTTCTACAGTATCGTTATCCTTGGGAGGGAACTGATCGCGATTATGAATACGAGGAGGTATTGCTATATTTGAAACTGTGAAATTTTACATTACTGTAGCACTCAATTACTATAGGATTATGTTTTGTATGAAATGGATCTTTTCTTGGGTTCTTTTCGAACTTTAGATTCCATCAAATGTCCCAAATTATTGAAGGCTATCCCTCACCTTTGATGGGTGAACGGGGTCAAGGTAATTCTCTTAGAAACTTTGTTTGAAAGGAACCAACGTAATTTTCAAGACAAGTTCCTTCCTTGGTTTGATTGTTTTGAGATTTGCTTGAGTTAAAATTTTTACTATATCATTAGTGTATTTATGTCATTGTATTTTCATTTGTTACTAAGACTCCTTTTGTTTCCTATGATGTACCTTGTGCATTACTCATTTTATTATTTCAATGAAAAGTCTTGTTTTCGTTTATATAAGAAAATTCCATTGAATGCCACTTCAATAAAAATAAATAAAAAATATATTTGTAATTTCACGAACAAGTTAAATGCAAAAGTTTAATCTTCATTTTTAAATGATTTTTTTAACATTTTTATTTTATTAGAAATCTCCAGTTTCATCTTTTACATTCTAGTTCAATTCATTCTATAATATATATATATATCAAATTCATTTCAAATTTTATTTACTAAACAGGTACGTTGTGGACCTTGGCTTTGGCTAACATTAGATAATGGATGCTTTATCGTTGTCTTGCTGTGCAGCTTCTTGGACGGGTATTCAACATCCTTAGAGAGAATAATCCAGAGCTTGCAGGCGATAGGCGTAGAACAGTTATGAGGCCTCCTCAAGTTCTTCGTGAAGGCACAAAGAAAACTGTTTTTGTCAATTTCATGGATCTATGTAAAACGTATGTGTTCATTAACCTTATATAACCATGTTTTGTTTTTACTAGATTTATCATACTATGGCGGAAGATTTTTGGCTGTTTGCACTTTAGATTATTATTATTATTATTTGTCAATCTGTTCTGTGTTATTCAATTCAACAAATAAGCATATATGATTATTCCATGATATGTGTTCATTAACCTTATATAACTTTTGTTTGCTGCATGCACGTTGTCTGTTTGAATACCCAACTTTTGTGGTTTTCTTTTGTGTGTGGCAGGGCCTTGTATGCTTTCATTTTCTCAATTATAGTTTGTTTTTTTGAAAAAGAAACAGAGTATTGACATTTCATGCTCATAGGTAGAGTTTGTGCTCTAGCATTTTTCTTGCAGTATATGACATGTATCCCTATCACATGGTTAGTTCTGGAGGTCTCGTTGATTTATAAAAACCTGTGATCTTTATGATTCTTGAAAGAATGAGTTTGAATGGTTGTTGAATTAAGCACATTCTTCACTAGTACAGATGGGAAGGCACATTTGGATAGGAAAGTTGCTTGTTTATCATTATGACAAGTGAATATAGTCAACACTGCAATCATCATATTTGTGTCCAGTGCTGAGTGTTGCTCCTTGCACCTTTGTTCTGTTCTTAATTTTTAACTGACAGTTGATCTGTAATTTCTAGTTTCAGATCTCACAGATAGTTCCCCAGAAGGGGGTGGGGGTCATTTCATTTACCCTCACGCACTGTTAGTTATGGTTATTTCTATATTTATGGTACATGCTGATTTTTGGAACTTGTCTCTCTCTGCAAATGAAATAAAATAGGATGCATCGGCAGCCAGACCATGTTATGGCTTTCTTACTAGCTGAACTTGGAACCAGTGGCTCTCTTGATGGACAGCAGAGACTAGTTGTTAAAGGAAGGTTTGCACCAAAGAACTTTGAAGGAATTCTGCGTCGATATGTCAGTAAGTGGCCTCTCATTGACTACCTCATGCTATTTGTCTACCAAATGTGATCTTAATTCAGAATGGATGTTTTCTCTGTTGCCAATTGTTTTAACTGGCTGGTCAAGGAGATTGGAATTTTTATGGAATATTAACAAATCTATTGATTGTCTTTCTCCTCCATTTTATCGTTTTTCGTGTTTTTAACTTCTAACAAATCTATCGATTGTCTTACTCAACTTATTCGTAATTTGTCCAATAGGGAGTAGTTGGATGGTGCTTTTTTTCTTTCTTTGCTTGAGAATCACTCTTTTAGGCATGAGAGAAGAGATGTGAGGGTTTAAAGACCCAATCCTTTGGTGTAAGTCCTTTCAGTGTCTTGCGGACCATTCTCCTTAAGGTGTCGGTCTTTTTGGTCATTTAGAGGATTAAGATTCCTAGGAAAGTACCTGACAAGTGTTACATGGTCGTGCGAACACGAAGGATCGTTCTTTGAGAAGCTCTCCTCACTTGTTGGGCTGTTCTGTTGTGTCCTCTGTTGGAAGGTGGAGGAAGATCTGGATCAAATTTTGGTGTTGTAAGTTTGTTAGTGTTGTTTGGGAGAGTTTCATCCAGGGGTTTGGCTTTTTGTTCACTCGTCAGTGGAGACTTATTGTTATGATCAAGTAGTTCCTCCTCAGTCCGCCTAGGGGAAGGAGATGTTTAATGTGGCTTGTGGGGATGTGTGCAATAGTGTGGGTTTTGTGGGGGGATTGAAATAGTAGGACTTTTAGGGGAGTTGATAAAGACCCTGTGAGGTTTGGTCTTTGGTTTGTTTTCATGTTTCTTTGTGTGCTTTGGTTTTGAAGAACTTTTGTAATTATTCTGCCAACTTGGACTCCCTTCATGAAGGGGTTACTTCTGGGGGCAGGGTTCCTTTGTTGCCATTGTATTTTTTCGTTTTTTCTCAATGAAACTTGTTATTCTTATGCATTGGTGAGGAGGATCATCAAATTTTAAGAAATGGGCTTGTTTGAATATGTTGTCGTTTTTTTAAAGCTTAATGGTTGAAGGTTTGGTCCACATTTAGATTCTGTGAGCTATGTGATGAAGCCTTTTCATAATTATTAGTTAGGACTTGTTGAGCTTGATTGGGAGCTGTTTTGGTAGTTTGCTACCTCTCCCTTTGGTGGGCCCTTTTTTTGTATGTCCTTGTATTCTTCAATTTTTTTTTCAATGAAAACTCGGTTGTTCATTTGAAAATATCTTAATAACCGTTGGCTAAGTCTCCACTGGAGTTACATCCTGTTATTAATTATGTGTGATTTGATTCACTTCTAATCTCTACCTCATTATAGTTTCCACCCTTCCCTCATTTATTTCTTTCCTTCAGATGAATATGTGATTTGCATTGGGTGCAAGAGTCCAGATACTATCCTTTCAAAGGAGAACCGGCTTTTCTTTCTCAGATGCGAGAAGGTAAGGTTTTTTCTTTTCTTTTATTTTTTTCAGTCTAGATGCATATGCTTATTGTATATTTGTGATTTTTGTGTAAAGCTTGTTTGTCGCTAATCTATGCAACGGCCGTAGGGGCATAATGATCTATATATTTATAAATTTGCTGTGGTTCTTTTGATTGCATTGTTGGGGGAAATGATGAATTGGAATATTTGTGTTCACTGTTAAATTTACTTTATCTGAGATCTGATATTTGAATAGATTATTTTTTATTTATCCTTTGCTTCTTCTTACTAGAATGGCAGCCGAGGATCTGACCTTTTCATGTGCTCGAAAATTTATTATAGCTTACGTTGTTTTGTTTTTAATCAACAGTGTGGTTCTGGACGATCAGTTGCTCCAATTAAAGCTGGGTTTGTTGCTCGTGTTGGCCGGAGGAACACTGGGACATGA

mRNA sequence

ATGAATAATCCATTTTTGGATCCTGGCTTCCACCTCTCCTTCTGTATTTTCTGTTACAACAAAGGAACAATGGCGGACGAAGTTCAGAATGATGTGAATGATCAGGTTGCAGATATTGCCCCCTTTGATCCAACTAAGAAGAAGAAGAAGAAGAAGGTTGTACTTCAGGATCCGACTGATGAGAGTGTTGACAAGTTAGCAGAGAAAACTGAAAGTTTGTCAGTTTCTGATGGTTTGGAGGCTACAACATTTTCTGGACTTAAAAAGAAGAAGAAAAAACCAGTAGAGGCAAGCTCCTTGAATGAAGATGGTGCAGATGCTACGGAGGATTTGGAAGATCAAGCGGGGGAAAATGAAGATCGTGAGGGACTTGTTCTACAGTATCGTTATCCTTGGGAGGGAACTGATCGCGATTATGAATACGAGGAGCTTCTTGGACGGGTATTCAACATCCTTAGAGAGAATAATCCAGAGCTTGCAGGCGATAGGCGTAGAACAGTTATGAGGCCTCCTCAAGTTCTTCGTGAAGGCACAAAGAAAACTGTTTTTGTCAATTTCATGGATCTATGTAAAACGATGCATCGGCAGCCAGACCATGTTATGGCTTTCTTACTAGCTGAACTTGGAACCAGTGGCTCTCTTGATGGACAGCAGAGACTAGTTGTTAAAGGAAGGTTTGCACCAAAGAACTTTGAAGGAATTCTGCGTCGATATGTCAATGAATATGTGATTTGCATTGGGTGCAAGAGTCCAGATACTATCCTTTCAAAGGAGAACCGGCTTTTCTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCAGTTGCTCCAATTAAAGCTGGGTTTGTTGCTCGTGTTGGCCGGAGGAACACTGGGACATGA

Coding sequence (CDS)

ATGAATAATCCATTTTTGGATCCTGGCTTCCACCTCTCCTTCTGTATTTTCTGTTACAACAAAGGAACAATGGCGGACGAAGTTCAGAATGATGTGAATGATCAGGTTGCAGATATTGCCCCCTTTGATCCAACTAAGAAGAAGAAGAAGAAGAAGGTTGTACTTCAGGATCCGACTGATGAGAGTGTTGACAAGTTAGCAGAGAAAACTGAAAGTTTGTCAGTTTCTGATGGTTTGGAGGCTACAACATTTTCTGGACTTAAAAAGAAGAAGAAAAAACCAGTAGAGGCAAGCTCCTTGAATGAAGATGGTGCAGATGCTACGGAGGATTTGGAAGATCAAGCGGGGGAAAATGAAGATCGTGAGGGACTTGTTCTACAGTATCGTTATCCTTGGGAGGGAACTGATCGCGATTATGAATACGAGGAGCTTCTTGGACGGGTATTCAACATCCTTAGAGAGAATAATCCAGAGCTTGCAGGCGATAGGCGTAGAACAGTTATGAGGCCTCCTCAAGTTCTTCGTGAAGGCACAAAGAAAACTGTTTTTGTCAATTTCATGGATCTATGTAAAACGATGCATCGGCAGCCAGACCATGTTATGGCTTTCTTACTAGCTGAACTTGGAACCAGTGGCTCTCTTGATGGACAGCAGAGACTAGTTGTTAAAGGAAGGTTTGCACCAAAGAACTTTGAAGGAATTCTGCGTCGATATGTCAATGAATATGTGATTTGCATTGGGTGCAAGAGTCCAGATACTATCCTTTCAAAGGAGAACCGGCTTTTCTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCAGTTGCTCCAATTAAAGCTGGGTTTGTTGCTCGTGTTGGCCGGAGGAACACTGGGACATGA

Protein sequence

MNNPFLDPGFHLSFCIFCYNKGTMADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATTFSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFFLRCEKCGSGRSVAPIKAGFVARVGRRNTGT*
Homology
BLAST of Chy12G213330 vs. ExPASy Swiss-Prot
Match: P55871 (Eukaryotic translation initiation factor 2 subunit beta OS=Malus domestica OX=3750 PE=2 SV=2)

HSP 1 Score: 438.7 bits (1127), Expect = 5.1e-122
Identity = 221/272 (81.25%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 24  MADEVQNDVNDQ-VADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEAT 83
           MAD+ QN+V D+ VADIAPFDPTKKKKKK+VV+QD TD+SV KLAEK E+ + S+G E +
Sbjct: 1   MADDNQNEVKDEVVADIAPFDPTKKKKKKEVVIQDTTDDSVGKLAEKAEACTASEGQE-S 60

Query: 84  TFSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQY-RYPWEGTDRDYEY 143
           TF+GLKKKKKKP+E S LNE+  DA EDL ++ GE+E+ EG+VL+   YPWEG+DRDY Y
Sbjct: 61  TFAGLKKKKKKPIETSILNEESGDAVEDLNERTGEDEEGEGIVLETPSYPWEGSDRDYTY 120

Query: 144 EELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 203
           EELL RVF ILRENNP+LAGDRRRTVMRPPQVLREGTKK VFVNFMDLCKTMHRQPDHVM
Sbjct: 121 EELLDRVFTILRENNPDLAGDRRRTVMRPPQVLREGTKKAVFVNFMDLCKTMHRQPDHVM 180

Query: 204 AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRL 263
           AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVIC+GC+SPDTILSKENRL
Sbjct: 181 AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICLGCQSPDTILSKENRL 240

Query: 264 FFLRCEKCGSGRSVAPIKAGFVARVGRRNTGT 294
           FFLRCEKCGSGRSVAP KAGF+ARVGRRN GT
Sbjct: 241 FFLRCEKCGSGRSVAPNKAGFMARVGRRNAGT 271

BLAST of Chy12G213330 vs. ExPASy Swiss-Prot
Match: O24473 (Eukaryotic translation initiation factor 2 subunit beta OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.3e-114
Identity = 211/272 (77.57%), Postives = 234/272 (86.03%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADE Q +  ++  +IAPFDPTKKKKKKKVV+QDP DE VDKLAEKTE LSV++  EA +
Sbjct: 1   MADEEQMERKEEATEIAPFDPTKKKKKKKVVIQDPADE-VDKLAEKTEGLSVTESGEA-S 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVL--QYRYPWEGTDRDYEY 143
           F GLKKKKKK VE      +  D  + L+DQ GE+E  EG+VL    +YPWEGTDRDY+Y
Sbjct: 61  FVGLKKKKKKLVELDPSLVEAGDGEDTLDDQVGEDEQGEGIVLGGATQYPWEGTDRDYKY 120

Query: 144 EELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 203
           +ELLGRVFNILRENNP+LAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQP+HVM
Sbjct: 121 DELLGRVFNILRENNPDLAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPEHVM 180

Query: 204 AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRL 263
            FLLAE+GTSGSLDGQQRLV+KGRFAPKNFE ILRRY+NEYVIC GCKSPDTILSKENRL
Sbjct: 181 MFLLAEMGTSGSLDGQQRLVIKGRFAPKNFEAILRRYINEYVICHGCKSPDTILSKENRL 240

Query: 264 FFLRCEKCGSGRSVAPIKAGFVARVGRRNTGT 294
           FFLRCE+CGS RSVAPIKAGFVA+VGRR  GT
Sbjct: 241 FFLRCEQCGSSRSVAPIKAGFVAQVGRRKAGT 270

BLAST of Chy12G213330 vs. ExPASy Swiss-Prot
Match: Q41969 (Eukaryotic translation initiation factor 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=EIF2B PE=1 SV=3)

HSP 1 Score: 409.1 bits (1050), Expect = 4.3e-113
Identity = 205/270 (75.93%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVD-KLAEKTESLSVSDGLEAT 83
           MADE+ N++ ++   +APFDP+KKKKKKKVV+Q+P ++  +    EK++SL V+DGLE +
Sbjct: 1   MADEI-NEIREEQEQLAPFDPSKKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGLE-S 60

Query: 84  TFSGLKKKKKKPVEASSLNEDGADATEDLEDQAG-ENEDREGLVLQYRYPWEGTDRDYEY 143
           +F+G+KKKKKKP E+S LN +  DA EDL++ A  E E  EG+VLQ RYPWEG++RDY Y
Sbjct: 61  SFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERDYIY 120

Query: 144 EELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 203
           +ELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM
Sbjct: 121 DELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 180

Query: 204 AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRL 263
            +LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRY+ +YVIC+GCKSPDTILSKENRL
Sbjct: 181 QYLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYITDYVICLGCKSPDTILSKENRL 240

Query: 264 FFLRCEKCGSGRSVAPIKAGFVARVGRRNT 292
           FFLRCEKCGS RSVAPIK GFVARV RR T
Sbjct: 241 FFLRCEKCGSQRSVAPIKTGFVARVSRRKT 268

BLAST of Chy12G213330 vs. ExPASy Swiss-Prot
Match: Q5E9D0 (Eukaryotic translation initiation factor 2 subunit 2 OS=Bos taurus OX=9913 GN=EIF2S2 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.4e-63
Identity = 130/266 (48.87%), Postives = 172/266 (64.66%), Query Frame = 0

Query: 26  DEVQNDVNDQVADIAPFDPTKKKKKKKVVLQ-DPTDESVDKLAEKTESLSVSDGLEATTF 85
           D  + D +D + D+  F+  KKKKK K +   D  +E +  L  +++    ++  E    
Sbjct: 60  DSRKKDASDDLDDLNFFNQKKKKKKSKKIFDIDEAEEGIKDLKIESDVQEPAEPEEDLDI 119

Query: 86  SGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEEL 145
               KKKKK V      ++  +  E LED+  + +D      Q    W G++RDY YEEL
Sbjct: 120 MLGNKKKKKKVVKFPDEDEVLEKDEALEDEDSKKDDGISFSNQTGPAWAGSERDYTYEEL 179

Query: 146 LGRVFNILRENNPEL-AGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 205
           L RVFNI+RE NP++ AG++R+ VM+PPQV+R GTKKT FVNF D+CK +HRQP H++AF
Sbjct: 180 LNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAF 239

Query: 206 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 265
           LLAELGTSGS+DG  +LV+KGRF  K  E +LRRY+ EYV C  C+SPDTIL K+ RL+F
Sbjct: 240 LLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYF 299

Query: 266 LRCEKCGSGRSVAPIKAGFVARVGRR 290
           L+CE C S  SVA IK GF A  GRR
Sbjct: 300 LQCETCHSRCSVASIKTGFQAVTGRR 325

BLAST of Chy12G213330 vs. ExPASy Swiss-Prot
Match: Q99L45 (Eukaryotic translation initiation factor 2 subunit 2 OS=Mus musculus OX=10090 GN=Eif2s2 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 9.1e-63
Identity = 133/269 (49.44%), Postives = 179/269 (66.54%), Query Frame = 0

Query: 26  DEVQNDVNDQVADIAPFDPTKKKKK-KKVVLQDPTDESVDKL---AEKTESLSVSDGLEA 85
           D  + D +D + D+  F+  KKKKK KK+   D  +E++  +   ++  E     D L+ 
Sbjct: 60  DSRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEAIKDVKIESDAQEPAEPEDDLD- 119

Query: 86  TTFSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEY 145
               G KKKKKK V+     ++  +  E LED+  + +D  G+    +  W G++RDY Y
Sbjct: 120 -IMLGNKKKKKKNVKFPE-EDEILEKDEALEDEDSKKDD--GISFSSQTAWAGSERDYTY 179

Query: 146 EELLGRVFNILRENNPEL-AGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHV 205
           EELL RVFNI+RE NP++ AG++R+ VM+PPQV+R GTKKT FVNF D+CK +HRQP H+
Sbjct: 180 EELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHL 239

Query: 206 MAFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENR 265
           +AFLLAELGTSGS+DG  +LV+KGRF  K  E +LRRY+ EYV C  C+SPDTIL K+ R
Sbjct: 240 LAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTR 299

Query: 266 LFFLRCEKCGSGRSVAPIKAGFVARVGRR 290
           L+FL+CE C S  SVA IK GF A  G+R
Sbjct: 300 LYFLQCETCHSRCSVASIKTGFQAVTGKR 323

BLAST of Chy12G213330 vs. ExPASy TrEMBL
Match: A0A0A0LY41 (eIF2B_5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G145930 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.6e-149
Identity = 269/270 (99.63%), Postives = 269/270 (99.63%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEA T
Sbjct: 1   MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEAAT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 294
           LRCEKCGSGRSVAPIKAGFVARVGRRNTGT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 270

BLAST of Chy12G213330 vs. ExPASy TrEMBL
Match: A0A1S3CG20 (eukaryotic translation initiation factor 2 subunit beta OS=Cucumis melo OX=3656 GN=LOC103500512 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.2e-145
Identity = 264/270 (97.78%), Postives = 265/270 (98.15%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADEVQNDV DQVADIAPFDPTKKKKKKKVVLQDPTDE VDKLAEKTESLSVSDGLE TT
Sbjct: 1   MADEVQNDVTDQVADIAPFDPTKKKKKKKVVLQDPTDEIVDKLAEKTESLSVSDGLETTT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEASSLNED ADATEDLEDQAGE EDREG+VLQYRYPWEGTDRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEASSLNEDSADATEDLEDQAGEIEDREGIVLQYRYPWEGTDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 294
           LRCEKCGSGRSVAPIKAGFVARVGRRNTGT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 270

BLAST of Chy12G213330 vs. ExPASy TrEMBL
Match: A0A6J1GBQ3 (eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111452713 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.8e-144
Identity = 258/270 (95.56%), Postives = 265/270 (98.15%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADE QNDVNDQVADIAPFDPTKKKKKKKVV+QDP DESVDKLAEKTESLSVSDGLE TT
Sbjct: 1   MADEAQNDVNDQVADIAPFDPTKKKKKKKVVIQDPADESVDKLAEKTESLSVSDGLEPTT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEA SLN+DGADATEDL+DQ+GENEDREG+VLQYR+PWEG+DRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEAISLNDDGADATEDLDDQSGENEDREGIVLQYRFPWEGSDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 294
           LRCEKCGSGRSVAPIKAGFVARVGRRN GT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNAGT 270

BLAST of Chy12G213330 vs. ExPASy TrEMBL
Match: A0A6J1KER3 (eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111492602 PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.4e-143
Identity = 257/270 (95.19%), Postives = 264/270 (97.78%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADE QNDVNDQVADIAPFDPTKKKKKKKVV+QDP DESVDKLAEKTESLSVSDGLE TT
Sbjct: 1   MADEAQNDVNDQVADIAPFDPTKKKKKKKVVIQDPADESVDKLAEKTESLSVSDGLEPTT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEA SLN+DG DATEDL+DQ+GENEDREG+VLQYR+PWEG+DRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEAISLNDDGPDATEDLDDQSGENEDREGIVLQYRFPWEGSDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 294
           LRCEKCGSGRSVAPIKAGFVARVGRRN GT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNAGT 270

BLAST of Chy12G213330 vs. ExPASy TrEMBL
Match: A0A6J1HIQ4 (eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111464801 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.4e-143
Identity = 258/270 (95.56%), Postives = 263/270 (97.41%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQD  DESVDKLAEKTESLSVSDGLE TT
Sbjct: 1   MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDLADESVDKLAEKTESLSVSDGLETTT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEA SL +DGADATEDLEDQAGENED++G+ LQYRYPWEG+DRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEAISLTDDGADATEDLEDQAGENEDQDGITLQYRYPWEGSDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 294
           LRCEKCGSGRSVAPIKAGFVARVGRRN GT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNAGT 270

BLAST of Chy12G213330 vs. NCBI nr
Match: XP_004139463.2 (eukaryotic translation initiation factor 2 subunit beta [Cucumis sativus] >KGN64891.1 hypothetical protein Csa_022839 [Cucumis sativus])

HSP 1 Score: 533 bits (1373), Expect = 1.87e-190
Identity = 269/270 (99.63%), Postives = 269/270 (99.63%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEA T
Sbjct: 1   MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEAAT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 293
           LRCEKCGSGRSVAPIKAGFVARVGRRNTGT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 270

BLAST of Chy12G213330 vs. NCBI nr
Match: XP_038894246.1 (eukaryotic translation initiation factor 2 subunit beta [Benincasa hispida] >XP_038894247.1 eukaryotic translation initiation factor 2 subunit beta [Benincasa hispida])

HSP 1 Score: 525 bits (1351), Expect = 4.22e-187
Identity = 263/270 (97.41%), Postives = 266/270 (98.52%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDE VDKLAEKTESLSVSDGLE TT
Sbjct: 1   MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDEIVDKLAEKTESLSVSDGLETTT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEASSLN+DGAD TEDLEDQ GENEDREG+VLQYRYPWEG+DRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEASSLNDDGADGTEDLEDQVGENEDREGIVLQYRYPWEGSDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 293
           LRCEKCGSGRSVAPIKAGFVARVGRRNTGT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 270

BLAST of Chy12G213330 vs. NCBI nr
Match: XP_008462075.1 (PREDICTED: eukaryotic translation initiation factor 2 subunit beta [Cucumis melo] >XP_008462082.1 PREDICTED: eukaryotic translation initiation factor 2 subunit beta [Cucumis melo])

HSP 1 Score: 522 bits (1345), Expect = 3.47e-186
Identity = 264/270 (97.78%), Postives = 265/270 (98.15%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADEVQNDV DQVADIAPFDPTKKKKKKKVVLQDPTDE VDKLAEKTESLSVSDGLE TT
Sbjct: 1   MADEVQNDVTDQVADIAPFDPTKKKKKKKVVLQDPTDEIVDKLAEKTESLSVSDGLETTT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEASSLNED ADATEDLEDQAGE EDREG+VLQYRYPWEGTDRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEASSLNEDSADATEDLEDQAGEIEDREGIVLQYRYPWEGTDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 293
           LRCEKCGSGRSVAPIKAGFVARVGRRNTGT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 270

BLAST of Chy12G213330 vs. NCBI nr
Match: KAG7037046.1 (hypothetical protein SDJN02_00667, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 523 bits (1346), Expect = 2.20e-185
Identity = 264/288 (91.67%), Postives = 273/288 (94.79%), Query Frame = 0

Query: 6   LDPGFHLSFCIFCYNKGTMADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDK 65
           L    HLS  +   + G MADE QNDVNDQVADIAPFDPTKKKKKKKVV+QDP DESVDK
Sbjct: 46  LSRPLHLSGAL---SSGIMADEAQNDVNDQVADIAPFDPTKKKKKKKVVIQDPADESVDK 105

Query: 66  LAEKTESLSVSDGLEATTFSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLV 125
           LAEKTESLSVSDGLEATTFSGLKKKKKKPVEA SLN+DGADATEDL+DQ+GENEDREG+V
Sbjct: 106 LAEKTESLSVSDGLEATTFSGLKKKKKKPVEAISLNDDGADATEDLDDQSGENEDREGIV 165

Query: 126 LQYRYPWEGTDRDYEYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVN 185
           LQYR+PWEG+DRDYEYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVN
Sbjct: 166 LQYRFPWEGSDRDYEYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVN 225

Query: 186 FMDLCKTMHRQPDHVMAFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVIC 245
           FMDLCKTMHRQPDHVMAFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVIC
Sbjct: 226 FMDLCKTMHRQPDHVMAFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVIC 285

Query: 246 IGCKSPDTILSKENRLFFLRCEKCGSGRSVAPIKAGFVARVGRRNTGT 293
           IGCKSPDTILSKENRLFFLRCEKCGSGRSVAPIKAGFVARVGRRN GT
Sbjct: 286 IGCKSPDTILSKENRLFFLRCEKCGSGRSVAPIKAGFVARVGRRNAGT 330

BLAST of Chy12G213330 vs. NCBI nr
Match: XP_022949327.1 (eukaryotic translation initiation factor 2 subunit beta-like [Cucurbita moschata])

HSP 1 Score: 517 bits (1332), Expect = 3.32e-184
Identity = 258/270 (95.56%), Postives = 265/270 (98.15%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGLEATT 83
           MADE QNDVNDQVADIAPFDPTKKKKKKKVV+QDP DESVDKLAEKTESLSVSDGLE TT
Sbjct: 1   MADEAQNDVNDQVADIAPFDPTKKKKKKKVVIQDPADESVDKLAEKTESLSVSDGLEPTT 60

Query: 84  FSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDYEYEE 143
           FSGLKKKKKKPVEA SLN+DGADATEDL+DQ+GENEDREG+VLQYR+PWEG+DRDYEYEE
Sbjct: 61  FSGLKKKKKKPVEAISLNDDGADATEDLDDQSGENEDREGIVLQYRFPWEGSDRDYEYEE 120

Query: 144 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 203
           LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF
Sbjct: 121 LLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAF 180

Query: 204 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 263
           LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF
Sbjct: 181 LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRLFF 240

Query: 264 LRCEKCGSGRSVAPIKAGFVARVGRRNTGT 293
           LRCEKCGSGRSVAPIKAGFVARVGRRN GT
Sbjct: 241 LRCEKCGSGRSVAPIKAGFVARVGRRNAGT 270

BLAST of Chy12G213330 vs. TAIR 10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 409.1 bits (1050), Expect = 3.1e-114
Identity = 205/270 (75.93%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVD-KLAEKTESLSVSDGLEAT 83
           MADE+ N++ ++   +APFDP+KKKKKKKVV+Q+P ++  +    EK++SL V+DGLE +
Sbjct: 1   MADEI-NEIREEQEQLAPFDPSKKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGLE-S 60

Query: 84  TFSGLKKKKKKPVEASSLNEDGADATEDLEDQAG-ENEDREGLVLQYRYPWEGTDRDYEY 143
           +F+G+KKKKKKP E+S LN +  DA EDL++ A  E E  EG+VLQ RYPWEG++RDY Y
Sbjct: 61  SFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERDYIY 120

Query: 144 EELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 203
           +ELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM
Sbjct: 121 DELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 180

Query: 204 AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRL 263
            +LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRY+ +YVIC+GCKSPDTILSKENRL
Sbjct: 181 QYLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYITDYVICLGCKSPDTILSKENRL 240

Query: 264 FFLRCEKCGSGRSVAPIKAGFVARVGRRNT 292
           FFLRCEKCGS RSVAPIK GFVARV RR T
Sbjct: 241 FFLRCEKCGSQRSVAPIKTGFVARVSRRKT 268

BLAST of Chy12G213330 vs. TAIR 10
Match: AT5G20920.3 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 409.1 bits (1050), Expect = 3.1e-114
Identity = 205/270 (75.93%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVD-KLAEKTESLSVSDGLEAT 83
           MADE+ N++ ++   +APFDP+KKKKKKKVV+Q+P ++  +    EK++SL V+DGLE +
Sbjct: 1   MADEI-NEIREEQEQLAPFDPSKKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGLE-S 60

Query: 84  TFSGLKKKKKKPVEASSLNEDGADATEDLEDQAG-ENEDREGLVLQYRYPWEGTDRDYEY 143
           +F+G+KKKKKKP E+S LN +  DA EDL++ A  E E  EG+VLQ RYPWEG++RDY Y
Sbjct: 61  SFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERDYIY 120

Query: 144 EELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 203
           +ELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM
Sbjct: 121 DELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 180

Query: 204 AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRL 263
            +LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRY+ +YVIC+GCKSPDTILSKENRL
Sbjct: 181 QYLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYITDYVICLGCKSPDTILSKENRL 240

Query: 264 FFLRCEKCGSGRSVAPIKAGFVARVGRRNT 292
           FFLRCEKCGS RSVAPIK GFVARV RR T
Sbjct: 241 FFLRCEKCGSQRSVAPIKTGFVARVSRRKT 268

BLAST of Chy12G213330 vs. TAIR 10
Match: AT5G20920.2 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 406.0 bits (1042), Expect = 2.6e-113
Identity = 205/270 (75.93%), Postives = 237/270 (87.78%), Query Frame = 0

Query: 24  MADEVQNDVNDQVADIAPFDPTKKKKKKKVVLQDPTDESVD-KLAEKTESLSVSDGLEAT 83
           MADE+ N++ ++  ++APFDP+KKKKKKKVV+Q+P ++  +    EK++SL V+DGLE +
Sbjct: 1   MADEI-NEIREE-QELAPFDPSKKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGLE-S 60

Query: 84  TFSGLKKKKKKPVEASSLNEDGADATEDLEDQAG-ENEDREGLVLQYRYPWEGTDRDYEY 143
           +F+G+KKKKKKP E+S LN +  DA EDL++ A  E E  EG+VLQ RYPWEG++RDY Y
Sbjct: 61  SFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERDYIY 120

Query: 144 EELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 203
           +ELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM
Sbjct: 121 DELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVM 180

Query: 204 AFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKENRL 263
            +LLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRY+ +YVIC+GCKSPDTILSKENRL
Sbjct: 181 QYLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYITDYVICLGCKSPDTILSKENRL 240

Query: 264 FFLRCEKCGSGRSVAPIKAGFVARVGRRNT 292
           FFLRCEKCGS RSVAPIK GFVARV RR T
Sbjct: 241 FFLRCEKCGSQRSVAPIKTGFVARVSRRKT 267

BLAST of Chy12G213330 vs. TAIR 10
Match: AT3G07920.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 213.8 bits (543), Expect = 1.9e-55
Identity = 105/148 (70.95%), Postives = 118/148 (79.73%), Query Frame = 0

Query: 142 EELLGRVFNILRENNPELAGDRRRTVMRPPQVLRE----GTKKTVFVNFMDLCKTMHRQP 201
           +E+L RVFNILREN+PEL G    T++ PPQVLRE    GTKKTVFVNFMD CKTMHR P
Sbjct: 17  QEILRRVFNILRENSPELVGIWLLTIIWPPQVLREETAKGTKKTVFVNFMDYCKTMHRNP 76

Query: 202 DHVMAFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSK 261
           DHVMAFLLAELGT G LD QQRLVV+GRF  ++FE +LR Y+ +YV+CI CKS + ILSK
Sbjct: 77  DHVMAFLLAELGTRGKLDDQQRLVVRGRFTQRDFESLLRGYILDYVMCIPCKSTEAILSK 136

Query: 262 ENRLFFLRCEKCGSGRSVAPIKAGFVAR 286
           ENRLFFLRC KCGSGR VAPIK  F  R
Sbjct: 137 ENRLFFLRCGKCGSGRPVAPIKINFGRR 164

BLAST of Chy12G213330 vs. TAIR 10
Match: AT5G01940.1 (eukaryotic translation initiation factor 2B family protein / eIF-2B family protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.2e-36
Identity = 88/184 (47.83%), Postives = 119/184 (64.67%), Query Frame = 0

Query: 88  KKKKKKPV--EASSLNEDGADATED-LEDQAGENEDREGLVLQYRYPWEGTDRDYEYEEL 147
           KKKK+KP+  E     ++G   TED L D     +                + DY Y+EL
Sbjct: 34  KKKKQKPLIREDDIFFQNGGHFTEDNLPDCQSSRK---------------FEPDYGYKEL 93

Query: 148 LGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMAFL 207
           L  VF+ LRE + E++ +R RTVM PPQ+L EGT  TV +NF DLC+TMHR+PDHVM FL
Sbjct: 94  LSMVFDRLREEDVEVSTERPRTVMMPPQLLAEGT-ITVCLNFADLCRTMHRKPDHVMKFL 153

Query: 208 LAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYVNEYVICIGCKSPDTILSKE-NRLFF 267
           LA++ T  SL+ QQRL +KG  + K+F+ + R+Y++ +VIC+ CKSPDT L++E N LF 
Sbjct: 154 LAQMETKVSLNKQQRLEIKGLVSSKDFQAVFRKYIDAFVICVCCKSPDTALAEEGNGLFN 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P558715.1e-12281.25Eukaryotic translation initiation factor 2 subunit beta OS=Malus domestica OX=37... [more]
O244732.3e-11477.57Eukaryotic translation initiation factor 2 subunit beta OS=Triticum aestivum OX=... [more]
Q419694.3e-11375.93Eukaryotic translation initiation factor 2 subunit beta OS=Arabidopsis thaliana ... [more]
Q5E9D05.4e-6348.87Eukaryotic translation initiation factor 2 subunit 2 OS=Bos taurus OX=9913 GN=EI... [more]
Q99L459.1e-6349.44Eukaryotic translation initiation factor 2 subunit 2 OS=Mus musculus OX=10090 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LY416.6e-14999.63eIF2B_5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G145930 PE=... [more]
A0A1S3CG201.2e-14597.78eukaryotic translation initiation factor 2 subunit beta OS=Cucumis melo OX=3656 ... [more]
A0A6J1GBQ33.8e-14495.56eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita moscha... [more]
A0A6J1KER31.4e-14395.19eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita maxima... [more]
A0A6J1HIQ45.4e-14395.56eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
XP_004139463.21.87e-19099.63eukaryotic translation initiation factor 2 subunit beta [Cucumis sativus] >KGN64... [more]
XP_038894246.14.22e-18797.41eukaryotic translation initiation factor 2 subunit beta [Benincasa hispida] >XP_... [more]
XP_008462075.13.47e-18697.78PREDICTED: eukaryotic translation initiation factor 2 subunit beta [Cucumis melo... [more]
KAG7037046.12.20e-18591.67hypothetical protein SDJN02_00667, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022949327.13.32e-18495.56eukaryotic translation initiation factor 2 subunit beta-like [Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
AT5G20920.13.1e-11475.93eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.33.1e-11475.93eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.22.6e-11375.93eukaryotic translation initiation factor 2 beta subunit [more]
AT3G07920.11.9e-5570.95Translation initiation factor IF2/IF5 [more]
AT5G01940.11.2e-3647.83eukaryotic translation initiation factor 2B family protein / eIF-2B family prote... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002735Translation initiation factor IF2/IF5SMARTSM00653eIF2Bneu4coord: 163..272
e-value: 6.8E-70
score: 248.2
IPR002735Translation initiation factor IF2/IF5PFAMPF01873eIF-5_eIF-2Bcoord: 162..271
e-value: 8.4E-39
score: 132.1
NoneNo IPR availableGENE3D3.30.30.170coord: 136..278
e-value: 4.7E-53
score: 180.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..117
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..122
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..72
NoneNo IPR availablePANTHERPTHR23001:SF25EUKARYOTIC TRANSLATION INITIATION FACTOR 2 BETA SUBUNITcoord: 24..293
NoneNo IPR availablePANTHERPTHR23001EUKARYOTIC TRANSLATION INITIATION FACTORcoord: 24..293
IPR016189Translation initiation factor IF2/IF5, N-terminalSUPERFAMILY100966Translation initiation factor 2 beta, aIF2beta, N-terminal domaincoord: 138..245
IPR016190Translation initiation factor IF2/IF5, zinc-bindingSUPERFAMILY75689Zinc-binding domain of translation initiation factor 2 betacoord: 242..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G213330.1Chy12G213330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001732 formation of cytoplasmic translation initiation complex
biological_process GO:0001731 formation of translation preinitiation complex
biological_process GO:0006413 translational initiation
cellular_component GO:0005850 eukaryotic translation initiation factor 2 complex
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0031369 translation initiation factor binding