Chy12G212670 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G212670
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	bifunctional nitrilase/nitrile hydratase NIT4A
Location	chrH12: 9670491 .. 9675259 (+)
RNA-Seq Expression	Chy12G212670
Synteny	Chy12G212670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGGTTCCTTTTCCTTGCTTCCAATTTCAATTTACTCGTTATGTACTACTCTTGCTCCGGCTGTTGCTTCGCTCACTAGCTCTTGGATCGCAATGGTCAGTTCAGAGTTTGGAATGAGTTCCAGATTGTTTTTGCTTGTGTTCTTCGAAGCAAGCTTATTAGTTGTTTTGGCTGTTTGTTTGCTTGGTTTTCTTTAATCTTACAAGTTGCTTTCCTTTCATCAGCTGATTTTGGTTGAATAATTTAATTAGTTTAAACTATTACATCCCCGATTACTTTTCTTGGTTTTAATTGTTTCTCTTGTCTGCGATCAATATGAATTTGCTGCTGTGGCCTAACATTGTGCTCCTGATTGTTCTTTTCTCCCTTAATTTTACTCGATTGGAGACATTGAAGAGAAAATGTACACGTGAAATACCACGGCTTAAAATGATTTAATTCGTAAGTTCCAAAAGCCATTAACAGAGTTGAGGATTGTCACACTTAGATGGCAAATATTTTAGTATATTATAGGGCAGCTACTAGCCTATGACCATGTGGGCCTTTTTTATTCTTCCTCTGTAATGGTAAAAAGAAATCTCACTCTGTTTTACATGAACAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCGCAGCGATTGATGTACCTGGTTAGTCTTTGTTTCCAGGTGGACTTTGTTTGACTCAATTTGAGTTGCTACTGTAAAGAGAAAAATCTTAGCGTCTTACCATGGATAATTTCAGGTTAAGTTTTGCTAGGCTCATTGTATAATTCTCATTCTAGAGGGCTGACTTAATTTCTCCATGAGTTTACCAAACTTGATTTCTGGTGATATATTAGTATTAAATTCTTTCATCGTAACATACACCTGTTCTTTATCATCTTTCATTCTTCAAATGGATGTCTCATATTAGGACAGTACAATAAGGAGCTACTGATGTCTGTCTATTCCAAGTGAAATACGTGTTTGTTGCTCATGAAATGTGAAATGGGACTATGATCTGTTTTAATGGCTCTTGCAATTTACAGTTAATGAATGACAAACAAGATTGCTTTAACTACTGGCATTTCTTAGTTCTATTAATTTCAAAATCTTCCTACTCTTGCTTACATCTAAAACCTAGACATAATATTGTAAAGTTCTGAAGGCTATAGCCAGTTCCTGTCTTAGTTGCTGTTTAGGTTTTTTTTGAAACTCTATTTTGATAGGCTTCAGTTCATGTATGAGGTTGCTTTACTTCCATTCATCCTAATACAATCTGGTTGGCCTTGTGTTTACTTCCATTTATCCTAATACAATCTGGTTGGCCTTTTGTTTACTTCCATTCATCTAAGTAATATCTTGTTTTCTCATAACTAATTGTTTCATCTATAAGATCATATGGCACATTCACAAAATAAACTGCTTGAAGTGCACCGGCTGTGGTTCTAATATATTAATTGATTGTTATTTTGATGACTATAGACGGTAATACATGCAGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGTTAGTTATTCATTGCTGGCAATAATGACATTCCTGACGTATTTGCGTATTTCAAATCTAGTTTTATTCTTTTTGTTTGACTGTAAGATGTTTATTAAACTTAAAATAGATAGCACTACAAAAATGTGATAGGAATGGTATTAGAGGGATACTAAGGGTATATTGGTAGGTAGTTAAAGAGTTTGTCAGAGAAGTTTGGTTATAAATATAACGAGTAAGGAAGAAAGGGGTAGACAATATTTTGGGAGTAAATAGGGCTTTGAATGATCTCAAGATTGGGAGGGTCTAAGTACCTCAAATTACATGGTCTATTTTGTATTTCTCTTATCTTTTATAGTTCAATATATTTGGGTTCTTCCAAAAGACAGACTAGAAACTATTACAGACTAAAGAGGCTCTAGTTGTTAAGAGATGGTAAAATGATGGTCAAACTCCAGAGTAGGGGATTATTGTGTCACTGCTCACTATTTACAGACACTGGTCTATCATTTTCAGCTTCTTTTCTCAGAATATACTTGTTTGGTTAATCATATTATGCACAACTTTTTTGTTTTATGTTTCTCTTTCAACTACATCAAGTTAAATAGTACTTTACACCTAAACCAAATGGAAATAATCATATAGAAGAAGGTTTTCATATGCTTGAAGTTCTACTGCTATGTTAAGGATAGAAGTTGGTATTTCTTCAAAGTTGCCTAGCTTCGTCTAAACATTCCTTTTCTTTTGCTTAACAGAAGTTGCTCACTAGTAAGTGACTTATCATGTTTGTAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTATGATGGAGAGGCACTTATTTCAGCAGATCTGGGTATGTCATCTTATCCTTCTGCTTATGGTGATCAGAGCTTTAGTATGTAGTCCACCATTATCATTTTAGAGCTTTAGTATGTAGTCCACCATTATCATTTTAAGTGGTTGATATAAGAGGTTTTATCTTGAGGAACCTATTGTTTGATTTGAGTCTCTATCTTCTTCAAGTGAGTACTAGGATTCTCTCTCCTGGATGATCTGATGCTCCTGACAGCCAACGAGTCTGAAAACCTTTATTCCAAGCTCTTATTCTAAGCTTAGCCATTTTTGTGTCAATATAAATGAACGGCCACTAGGATTTAGTTCCAATAAAATGGTTAAGGATCAATCAACCATTAAAGAGAGGCAGGGCCTCTCTTGCTGTCTAAAAAGTTCCTTGGAGAATGTGGGGATAATCTGAAGTGGTTTTTTTTAACCTTACGGGCCTTGAGTTGTGAAACTTTTTCATCTAGATCTGTGTGAGCAAAGGTCCAACTAGGAATTCCTAAGCTAAGCCTCTTACCAAGGTAGAAGGGTTTTTGTGAAGGTTCTACTTGATAATACAAATAGAAAGCACAAAATTACATGGACAAGGCAATAACATCAATCTAACCACGAAAGGCCGAATGAAGGCCCAATCAAATCCCTTGGGGGAAAATTTTGATTTACCCCTTCTAACATGTCTGCACTATGTGGTCCTCACAGTTCGAATATTGAAGAATGTTAGATACCAAAGAGAGCTCCTAATATTTGACAGGCCAGGAAGACAAGAAGCAATAAAGCAAGAAAGAGTTATCGGCTTATCGCTATCGGATTTTTAATCTCCTATGACAGTCCCTCTTAGAAAAACACAAATACAAAAAAGAACCCCTCCTAAGATCGGTCACTAATGCCAGCTACTTAACCATAAGAGATTGGTCTTCAAGCCAAACATATTCTTAAATGAATAAAGGATTGAAGACGGATATTCAAATCTTCCAATCATATGCCTGCAAATAAAGGTTGGAGAGATAAACCTGGTTCCAACTGATTTCAGATAATTTTAGGATTAGCCACCTGGATAATAATAGTTCCAGTCTTTCAATTAGCTCTCTACTATATGCTAAAGCACCCATGATATGCTATTTCAGAATGGAGTTCATAGGGTTAAGTATATCAAAAGTACTTCTGCGGCCAAAACTCATAAAAATGAGAGAACATTTTGACTAGTTAAAAATTCCTTAAGCAAATAAAGAACATTTGAATATTAATTTGTTTCCTATTTAACAAATGGAGTAAAAGGCGTTTATAAGTTTTCACATGGTCTATTTATGAATGGTGAAATACCAAGTTTGAAATTGTGGCAAGCCATTGATTTTTGGCACGGTTCAGTATGAATGATTATTGATCCATCCACCCCAACTCTTAACAAATTAAACTTTTTGGGGCATATGTTTTATTGGCTATTTACCCCTCCCTTCATTGATTGTTATGGAAAGCTTTTTTCTACGACTAGCATTTTTAGTGAAAAATAATTTCGATGAATATCTAACAGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGCCAAAGATTCTTGTAAAAAGTAG

mRNA sequence

ATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGGTTCCTTTTCCTTGCTTCCAATTTCAATTTACTCGTTATGTACTACTCTTGCTCCGGCTGTTGCTTCGCTCACTAGCTCTTGGATCGCAATGGTCAGTTCAGAGTTTGGAATGAGTTCCAGATTGTTTTTGCTTGTGTTCTTCGAAGCAAGCTTATTAGTTGTTTTGGCTGTTTGTTTGCTTGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCGCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTATGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGCCAAAGATTCTTGTAAAAAGTAG

Coding sequence (CDS)

ATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGGTTCCTTTTCCTTGCTTCCAATTTCAATTTACTCGTTATGTACTACTCTTGCTCCGGCTGTTGCTTCGCTCACTAGCTCTTGGATCGCAATGGTCAGTTCAGAGTTTGGAATGAGTTCCAGATTGTTTTTGCTTGTGTTCTTCGAAGCAAGCTTATTAGTTGTTTTGGCTGTTTGTTTGCTTGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCGCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTATGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGCCAAAGATTCTTGTAAAAAGTAG

Protein sequence

MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSLCTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK*

Homology

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BLAST of Chy12G212670 vs. ExPASy Swiss-Prot
Match: Q42965 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 4.1e-170
Identity = 303/410 (73.90%), Postives = 324/410 (79.02%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPT A  +  L AEVDMG +SS   VRATVVQASTIFYDTPAT              
Sbjct: 1   MALVPTPAVNEGPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          L KAERLLAEAA 
Sbjct: 61  -----------------------------------------------LVKAERLLAEAAS 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           +G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 121 YGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KY+V+LVMGVIERDGYTLYCTVLFFD QG +LGKHRK+MPTALERIIWGFGDGSTIPV++
Sbjct: 181 KYKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYD 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           T +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSA
Sbjct: 241 TPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPPEYVFSGTEE+LTPDS+VCAGGS IISPSG +LAGPNY GEALISADL
Sbjct: 301 NQFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADL 349

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCK 411
           DLGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+KA+ S K
Sbjct: 361 DLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349

[Close]

BLAST of Chy12G212670 vs. ExPASy Swiss-Prot
Match: Q42966 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.6e-169
Identity = 302/410 (73.66%), Postives = 324/410 (79.02%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPT    +  + AEVDMG +SS   VRATVVQASTIFYDTPAT              
Sbjct: 1   MALVPTPVVNEGPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          LDKAERLLAEAA 
Sbjct: 61  -----------------------------------------------LDKAERLLAEAAS 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           +G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 121 YGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KY+V+LVMGVIERDGYTLYCTVLFFD QG YLGKHRK+MPTALERIIWGFGDGSTIPV++
Sbjct: 181 KYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYD 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           T +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSA
Sbjct: 241 TPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPPEYVFSGT E+LTPDS+VCAGGS IISPSG +LAGPNY+GEALISADL
Sbjct: 301 NQFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADL 348

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCK 411
           DLGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+KA+ S K
Sbjct: 361 DLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348

[Close]

BLAST of Chy12G212670 vs. ExPASy Swiss-Prot
Match: Q5QGZ8 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 7.8e-169
Identity = 299/410 (72.93%), Postives = 321/410 (78.29%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALV T    D  L AEV+M SD +   VRATVVQASTIFYDTPAT              
Sbjct: 1   MALVTTPTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          LDKAERLLAEAA 
Sbjct: 61  -----------------------------------------------LDKAERLLAEAAS 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           +G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKE+FRKYH+AAIDVPGPEVDRLAA+AG
Sbjct: 121 YGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KY+V+LVMGVIERDGYTLYCTVLFF  QGRYLGKHRK+MPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYKVYLVMGVIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           T IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSA
Sbjct: 241 TPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRR+DYPPPPEYVF GTEE LTPDSVVCAGGS IISPSG +LAGP+Y+GEALISADL
Sbjct: 301 NQFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADL 349

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCK 411
           DLGEIARAKFDFDVVGHY+RPEVLSLVV+DHPT PVTFTS STK +D  K
Sbjct: 361 DLGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIEDKTK 349

[Close]

BLAST of Chy12G212670 vs. ExPASy Swiss-Prot
Match: Q3LRV4 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 8.6e-168
Identity = 303/412 (73.54%), Postives = 323/412 (78.40%), Query Frame = 0


Query: 1   MALVPTTAPT--DSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIY 60
           MALV TT PT  D  L AEVDM S  +   VRATVVQASTIFYDTPAT            
Sbjct: 1   MALV-TTTPTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPAT------------ 60

Query: 61  SLCTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEA 120
                                                            LDKAERLL +A
Sbjct: 61  -------------------------------------------------LDKAERLLVQA 120

Query: 121 AGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAM 180
           A +G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKEEFRKYH+AAIDVPGPEVDRL+AM
Sbjct: 121 ASYGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAM 180

Query: 181 AGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPV 240
           AGKY+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPV
Sbjct: 181 AGKYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPV 240

Query: 241 FETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVL 300
           F+T IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSRD WQAS THIALEGGCFVL
Sbjct: 241 FQTPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVL 300

Query: 301 SANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISA 360
           SANQFCRRKDYPPPPEYVFSGTEE+LTPDSVV AGGS IISPSG +LAGPNY+GEALISA
Sbjct: 301 SANQFCRRKDYPPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISA 350

Query: 361 DLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCK 411
           DLDLGEIARAKFDFDVVGHY+R EVLSL+V+DHPT PVTFTSTSTK +D  K
Sbjct: 361 DLDLGEIARAKFDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIEDQTK 350

[Close]

BLAST of Chy12G212670 vs. ExPASy Swiss-Prot
Match: Q6H849 (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX=39947 GN=NIT4 PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 5.4e-154
Identity = 280/410 (68.29%), Postives = 307/410 (74.88%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDM--GSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIY 60
           MA+VP+ +     +IAEV+M  G+DS    VRATVVQAST+FYDTPAT            
Sbjct: 1   MAMVPSGSGGGPPVIAEVEMNGGADSGAATVRATVVQASTVFYDTPAT------------ 60

Query: 61  SLCTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEA 120
                                                            LDKAERL+ EA
Sbjct: 61  -------------------------------------------------LDKAERLIEEA 120

Query: 121 AGFGSQLVVFPEAFVGGYPRGSNFG----VSIGNRTPKGKEEFRKYHAAAIDVPGPEVDR 180
           AG+GSQLVVFPEAFVGGYPRGS FG    +SIGN   KGKEEFRKYHAAAI+VPGPEV R
Sbjct: 121 AGYGSQLVVFPEAFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTR 180

Query: 181 LAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGS 240
           LAAMAGKY+V LVMGVIER+GYTLYC+VLFFDP GRYLGKHRK+MPTALERIIWGFGDGS
Sbjct: 181 LAAMAGKYKVFLVMGVIEREGYTLYCSVLFFDPLGRYLGKHRKLMPTALERIIWGFGDGS 240

Query: 241 TIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGG 300
           TIPV++T +GKIGA ICWEN+MPLLRTA+Y KGIEIYCAPTADSR  WQASMTHIALEGG
Sbjct: 241 TIPVYDTPLGKIGALICWENKMPLLRTALYGKGIEIYCAPTADSRQVWQASMTHIALEGG 300

Query: 301 CFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEA 360
           CFVLSANQFCRRKDYPPPPEYVFSG  EE +PD+VVC GGS IISPSG +LAGPNY+GEA
Sbjct: 301 CFVLSANQFCRRKDYPPPPEYVFSGLGEEPSPDTVVCPGGSVIISPSGEVLAGPNYEGEA 349

Query: 361 LISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK 405
           LI+ADLDLGEI RAKFDFDVVGHYARPEVLSLVV D P  PV+FTS + K
Sbjct: 361 LITADLDLGEIVRAKFDFDVVGHYARPEVLSLVVNDQPHLPVSFTSAAEK 349

[Close]

BLAST of Chy12G212670 vs. ExPASy TrEMBL
Match: A0A0A0LVI0 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.1e-192
Identity = 347/411 (84.43%), Postives = 349/411 (84.91%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPAT              
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          LDKAERLLAEAAG
Sbjct: 61  -----------------------------------------------LDKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 412
           DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK +DSCKK
Sbjct: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Chy12G212670 vs. ExPASy TrEMBL
Match: A0A5A7SY83 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002030 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.7e-190
Identity = 344/411 (83.70%), Postives = 346/411 (84.18%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPAT              
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          LDKAERLLAEAAG
Sbjct: 61  -----------------------------------------------LDKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 412
           DLGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTKA+DSCKK
Sbjct: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Chy12G212670 vs. ExPASy TrEMBL
Match: A0A1S3CLB4 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.7e-190
Identity = 344/411 (83.70%), Postives = 346/411 (84.18%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPAT              
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          LDKAERLLAEAAG
Sbjct: 61  -----------------------------------------------LDKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 412
           DLGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTKA+DSCKK
Sbjct: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Chy12G212670 vs. ExPASy TrEMBL
Match: A0A6J1I7W0 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LOC111471660 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.7e-185
Identity = 335/411 (81.51%), Postives = 340/411 (82.73%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTA  D+QLIAEVDMGSD SV  VRATVVQASTIFYDTPAT              
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          LDKAERLLAEAAG
Sbjct: 61  -----------------------------------------------LDKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 412
           DLG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS S K +DSC K
Sbjct: 361 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

[Close]

BLAST of Chy12G212670 vs. ExPASy TrEMBL
Match: A0A6J1EHI6 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=LOC111434356 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 8.0e-185
Identity = 334/410 (81.46%), Postives = 340/410 (82.93%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTA  D+QLIAEVDMGSD SV  VRATVVQASTIFYDTPAT              
Sbjct: 1   MALVPTTAAADTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPAT-------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                          LDKAERLLAEAAG
Sbjct: 61  -----------------------------------------------LDKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 349

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCK 411
           DLG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS + K +DSCK
Sbjct: 361 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

[Close]

BLAST of Chy12G212670 vs. NCBI nr
Match: XP_004139500.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_022889 [Cucumis sativus])

HSP 1 Score: 680 bits (1754), Expect = 3.52e-245
Identity = 347/411 (84.43%), Postives = 349/411 (84.91%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATL             
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATL------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                           DKAERLLAEAAG
Sbjct: 61  ------------------------------------------------DKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 411
           DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK +DSCKK
Sbjct: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Chy12G212670 vs. NCBI nr
Match: XP_008463648.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo] >KAA0035558.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo var. makuwa] >TYK30976.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo var. makuwa])

HSP 1 Score: 673 bits (1736), Expect = 1.95e-242
Identity = 344/411 (83.70%), Postives = 346/411 (84.18%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATL             
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATL------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                           DKAERLLAEAAG
Sbjct: 61  ------------------------------------------------DKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 411
           DLGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTKA+DSCKK
Sbjct: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Chy12G212670 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 662 bits (1708), Expect = 3.59e-238
Identity = 338/411 (82.24%), Postives = 343/411 (83.45%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATL             
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATL------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                           DKAERLLAEAAG
Sbjct: 61  ------------------------------------------------DKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 411
           DLG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS STK +DSCKK
Sbjct: 361 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCKK 350

[Close]

BLAST of Chy12G212670 vs. NCBI nr
Match: KAG6583706.1 (Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019353.1 Bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 660 bits (1702), Expect = 2.94e-237
Identity = 336/411 (81.75%), Postives = 343/411 (83.45%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSL 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATL             
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATL------------- 60

Query: 61  CTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAG 120
                                                           DKAERLLAEAAG
Sbjct: 61  ------------------------------------------------DKAERLLAEAAG 120

Query: 121 FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180
           FGSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 121 FGSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 180

Query: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240
           KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE
Sbjct: 181 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 240

Query: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300
           TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 241 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 300

Query: 301 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 360
           NQFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADL
Sbjct: 301 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 350

Query: 361 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 411
           DLG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS +TK +DSCKK
Sbjct: 361 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 350

[Close]

BLAST of Chy12G212670 vs. NCBI nr
Match: XP_038894429.1 (bifunctional nitrilase/nitrile hydratase NIT4A isoform X1 [Benincasa hispida])

HSP 1 Score: 657 bits (1695), Expect = 3.56e-236
Identity = 336/412 (81.55%), Postives = 344/412 (83.50%), Query Frame = 0


Query: 1   MALVPTTAPTD-SQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYS 60
           MALVPTTA  D SQLIAEVDMGSDSSV+ VRATVVQASTIFYDTPATL            
Sbjct: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATL------------ 60

Query: 61  LCTTLAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAA 120
                                                            +KAERLLAEAA
Sbjct: 61  -------------------------------------------------EKAERLLAEAA 120

Query: 121 GFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMA 180
           GFGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMA
Sbjct: 121 GFGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMA 180

Query: 181 GKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 240
           GKYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVF
Sbjct: 181 GKYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVF 240

Query: 241 ETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLS 300
           ETSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLS
Sbjct: 241 ETSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLS 300

Query: 301 ANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISAD 360
           ANQFCRRKDYPPPPEYVFSGTEEEL+PDSVVCAGGSAIISPSGTILAGPNYDGEALISAD
Sbjct: 301 ANQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISAD 351

Query: 361 LDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDSCKK 411
           LDLGEI RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTSTSTKA+DSCKK
Sbjct: 361 LDLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 351

[Close]

BLAST of Chy12G212670 vs. TAIR 10
Match: AT5G22300.1 (nitrilase 4 )

HSP 1 Score: 525.8 bits (1353), Expect = 3.2e-149
Identity = 267/405 (65.93%), Postives = 302/405 (74.57%), Query Frame = 0


Query: 6   TTAPTDS--QLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLGSFSLLPISIYSLCTT 65
           T AP  +  Q+  E+DM +  S   VRATVVQAST+FYDTPAT                 
Sbjct: 11  TAAPQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPAT----------------- 70

Query: 66  LAPAVASLTSSWIAMVSSEFGMSSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAGFGS 125
                                                       LDKAERLL+EAA  GS
Sbjct: 71  --------------------------------------------LDKAERLLSEAAENGS 130

Query: 126 QLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYR 185
           QLVVFPEAF+GGYPRGS F ++IG+RT KG+++FRKYHA+AIDVPGPEV+RLA MA KY+
Sbjct: 131 QLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYK 190

Query: 186 VHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSI 245
           V+LVMGVIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T I
Sbjct: 191 VYLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPI 250

Query: 246 GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQF 305
           GKIGAAICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQF
Sbjct: 251 GKIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQF 310

Query: 306 CRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLG 365
           CRRKDYP PPEY+FSG+EE LTPDSVVCAGGS+IISP G +LAGPNY GEALI+ADLDLG
Sbjct: 311 CRRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLG 354

Query: 366 EIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKDS 409
           +IARAKFDFDVVGHY+RPEV SL +R+HP   V+F ++     +S
Sbjct: 371 DIARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSFKTSKVMEDES 354

[Close]

BLAST of Chy12G212670 vs. TAIR 10
Match: AT3G44320.1 (nitrilase 3 )

HSP 1 Score: 438.3 bits (1126), Expect = 6.6e-123
Identity = 200/304 (65.79%), Postives = 245/304 (80.59%), Query Frame = 0


Query: 108 LDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDV 167
           LDKAE+ + EAA  G++LV+FPEAF+GGYPRG  FG+++G    +G++EFR YHA+AI V
Sbjct: 43  LDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAVGVHNEEGRDEFRNYHASAIKV 102

Query: 168 PGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERII 227
           PGPEV+RLA +AGK  VHLVMG IE+DGYTLYCT LFF PQG++LGKHRKVMPT+LER I
Sbjct: 103 PGPEVERLAELAGKNNVHLVMGAIEKDGYTLYCTALFFSPQGQFLGKHRKVMPTSLERCI 162

Query: 228 WGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMT 287
           WG GDGSTIPV++T IGKIGAAICWENRMPL RTA+YAKGIEIYCAPTAD    WQASM 
Sbjct: 163 WGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYAKGIEIYCAPTADYSLEWQASMI 222

Query: 288 HIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAG 347
           HIA+EGGCFVLSA+QFC+R+++P  P+Y+F+   +    D  V  GGS IISP G +LAG
Sbjct: 223 HIAVEGGCFVLSAHQFCKRREFPEHPDYLFNDIVDTKEHDPTVSGGGSVIISPLGKVLAG 282

Query: 348 PNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKD 407
           PNY+ E L++ADLDLG+IARAK  FDVVGHY++P++ +L V +HP  PVTF +   KA+D
Sbjct: 283 PNYESEGLVTADLDLGDIARAKLYFDVVGHYSKPDIFNLTVNEHPKKPVTFMTKVEKAED 342

Query: 408 SCKK 412
              K
Sbjct: 343 ESNK 346

[Close]

BLAST of Chy12G212670 vs. TAIR 10
Match: AT3G44300.1 (nitrilase 2 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.5e-122
Identity = 205/326 (62.88%), Postives = 252/326 (77.30%), Query Frame = 0


Query: 86  SSRLFLLVFFEASLLVVLAVCLLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVS 145
           SS +      +AS +       L+KA + + EAA  GS+LVVFPEAF+GGYPRG  FG+ 
Sbjct: 14  SSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLG 73

Query: 146 IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFF 205
           +G    +G++EFRKYHA+AI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LFF
Sbjct: 74  VGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFF 133

Query: 206 DPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYA 265
            PQG++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YA
Sbjct: 134 SPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYA 193

Query: 266 KGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELT 325
           KGIE+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  P+Y+F+   ++  
Sbjct: 194 KGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKE 253

Query: 326 PDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLS 385
           PDS+V  GGS IISP G +LAGPN++ E LI+ADLDLG++ARAK  FD VGHY+RP+VL 
Sbjct: 254 PDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLH 313

Query: 386 LVVRDHPTTPVTFTSTSTKAKDSCKK 412
           L V +HP  PVTF S   KA+D   K
Sbjct: 314 LTVNEHPKKPVTFISKVEKAEDDSNK 339

[Close]

BLAST of Chy12G212670 vs. TAIR 10
Match: AT3G44310.1 (nitrilase 1 )

HSP 1 Score: 425.2 bits (1092), Expect = 5.8e-119
Identity = 194/304 (63.82%), Postives = 243/304 (79.93%), Query Frame = 0


Query: 108 LDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDV 167
           +DKAE+ + EAA  G++LV+FPE F+GGYPRG  FG+++G    +G++EFRKYHA+AI V
Sbjct: 43  IDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHV 102

Query: 168 PGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERII 227
           PGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQG++LGKHRK+MPT+LER I
Sbjct: 103 PGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQGQFLGKHRKLMPTSLERCI 162

Query: 228 WGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMT 287
           WG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE+YCAPTAD    WQ+SM 
Sbjct: 163 WGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSML 222

Query: 288 HIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAG 347
           HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS+V  GGS IISP G +LAG
Sbjct: 223 HIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAG 282

Query: 348 PNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKD 407
           PN++ E L++AD+DLG+IARAK  FD VGHY+RP+VL L V +HP   VTF +   KA+D
Sbjct: 283 PNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTVNEHPRKSVTFVTKVEKAED 342

Query: 408 SCKK 412
              K
Sbjct: 343 DSNK 346

[Close]

BLAST of Chy12G212670 vs. TAIR 10
Match: AT3G44310.3 (nitrilase 1 )

HSP 1 Score: 425.2 bits (1092), Expect = 5.8e-119
Identity = 194/304 (63.82%), Postives = 243/304 (79.93%), Query Frame = 0


Query: 108 LDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDV 167
           +DKAE+ + EAA  G++LV+FPE F+GGYPRG  FG+++G    +G++EFRKYHA+AI V
Sbjct: 43  IDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHV 102

Query: 168 PGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERII 227
           PGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQG++LGKHRK+MPT+LER I
Sbjct: 103 PGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQGQFLGKHRKLMPTSLERCI 162

Query: 228 WGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMT 287
           WG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE+YCAPTAD    WQ+SM 
Sbjct: 163 WGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSML 222

Query: 288 HIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAG 347
           HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS+V  GGS IISP G +LAG
Sbjct: 223 HIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAG 282

Query: 348 PNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAKD 407
           PN++ E L++AD+DLG+IARAK  FD VGHY+RP+VL L V +HP   VTF +   KA+D
Sbjct: 283 PNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTVNEHPRKSVTFVTKVEKAED 342

Query: 408 SCKK 412
              K
Sbjct: 343 DSNK 346

The following BLAST results are available for this feature:

BLAST of Chy12G212670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42965	4.1e-170	73.90	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N...	[more]
Q42966	1.6e-169	73.66	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N...	[more]
Q5QGZ8	7.8e-169	72.93	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ...	[more]
Q3LRV4	8.6e-168	73.54	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ...	[more]
Q6H849	5.4e-154	68.29	Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX...	[more]

BLAST of Chy12G212670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVI0	3.1e-192	84.43	CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G16836...	[more]
A0A5A7SY83	3.7e-190	83.70	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3CLB4	3.7e-190	83.70	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1I7W0	4.7e-185	81.51	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1EHI6	8.0e-185	81.46	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Chy12G212670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139500.1	3.52e-245	84.43	bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hyp...	[more]
XP_008463648.1	1.95e-242	83.70	PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo] >KAA003...	[more]
XP_023520715.1	3.59e-238	82.24	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo]	[more]
KAG6583706.1	2.94e-237	81.75	Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma ...	[more]
XP_038894429.1	3.56e-236	81.55	bifunctional nitrilase/nitrile hydratase NIT4A isoform X1 [Benincasa hispida]	[more]

BLAST of Chy12G212670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G22300.1	3.2e-149	65.93	nitrilase 4	[more]
AT3G44320.1	6.6e-123	65.79	nitrilase 3	[more]
AT3G44300.1	1.5e-122	62.88	nitrilase 2	[more]
AT3G44310.1	5.8e-119	63.82	nitrilase 1	[more]
AT3G44310.3	5.8e-119	63.82	nitrilase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	3.60.110.10		coord: 104..374 e-value: 7.7E-81 score: 273.2
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 108..388
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 108..369 e-value: 2.4E-65 score: 220.5
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 92..362 score: 49.612431
IPR044149	Nitrilase/Cyanide hydratase	PANTHER	PTHR46044	NITRILASE	coord: 13..404
IPR044149	Nitrilase/Cyanide hydratase	CDD	cd07564	nitrilases_CHs	coord: 29..390 e-value: 2.92585E-151 score: 428.06
None	No IPR available	PANTHER	PTHR46044:SF1	NITRILASE	coord: 13..404
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00921	NITRIL_CHT_2	coord: 247..260
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00920	NITRIL_CHT_1	coord: 122..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G212670.1	Chy12G212670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051410	detoxification of nitrogen compound
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0047427	cyanoalanine nitrilase activity
molecular_function	GO:0018822	nitrile hydratase activity
molecular_function	GO:0003824	catalytic activity