Chy12G211070 (gene) Cucumber (hystrix) v1

Overview
NameChy12G211070
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionGlutathione S-transferase family protein
LocationchrH12: 6906249 .. 6907868 (-)
RNA-Seq ExpressionChy12G211070
SyntenyChy12G211070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACGCTCAGCGCTCGATGAAATGTCTACAACAGGTGCTTTTGTAAGAACAGCTTCGACGTTTCGCAATTTGATTTCACGAGATGGATCCTCCCAGTTTCCATCAGAATCTGGGAGATACCATCTCTACATATCATATGCTTGTCCTTGGGCTTCCAGGTGCCTTGCCTATTTGAAAATCAAAGGACTCGAAAAAGCGATCAGTTTCACGGTACATTTCTTTTTTAATAGCTGAATGAAACCACGATGTTTTTACTGAGAACTAATCTCTTCTTTACATCAAACTATTTTTCTCAGTCAGTCAAACCAATATGGGAAAGAACCAAGGACTCTGATGATCATATGGGATGGGTTTTTCCTAGCTCTGATACAGAGGAGCCTGGTGCCGAACCTGACCCTTTGAATGGAGTTAGAAGTCTTAGAGAACTCTATGAACTTGCAAGTACAAATTACAGTGGGAAGTATACAGTTCCAGTAAGTTTCAGCCATTGTTGCGTTCTTAATTTGCGCTTCTTACTAGGTTTTATCATGTTATGCAGTCTTCTCAGTATCTTCCTCATGACAGGTTTTATGGGATATGAAACTCAAAACAATTGTTAACAATGAGAGCTCAGAGATAATTCGCATGCTTAATACTGAATTCAATGATATAGCTGAGAATCCATATCTGGATCTCTATCCCACTCACTTGCAAGCCCAAATTGATGAGACTAATGATTGGATATACCGTGGCATTAATAATGGTGTTTATAAATGTGGTTTTGCAAGGCAGCAACAGCCTTATGACCAGGTAAGCATTCTGTTTAGAAGCCAACAATTTAGAGTGCTCAAAGCAAAAACTGATTGAATGTATGAGAATACAAAGTGTTTCAATTACGTCACTATAGGATTGTTCTGATTCAAATAAGTTGAATTTTTAAGGTCTATGATTTCTTTTCAATTTTGTTTCAGCTGAATTTATCCCAGTTGGAATTAATTAACCCTTAAGGTCTATGCTTTTACATATTTTTTTACACGAACATTTCTAGTGATTTGTTTGATTCAAGTAGTATTTTGGATCAGAAATGTGCCCTTATACAATGCAACCCGATCAATAAACACTCGATCTTGAAAATTTAAACTTAGTCTATTTTGATGTACTCTAGAGATATAAATAACCAGGAACCATCCTTCTACAGGCTGTGAAAGAGTTATATGAAGCTTTAGACAGATGTGAGGAAATACTTTCGAAGCAACGTTACCTCTGTGGGAACACACTGACTGAAGCAGACATTCGGCTGTTTGTCACCATTATAAGATTCGACGAGGTACCATTATCAACTAACTCTCTCAAAAAAGTGCTTTTCTTTCTCCATCTGACCATCATTCTGTTTTCCAAGTTCAGGTTTATGCAGTTCACTTCAAATGCAACAAGAAACTACTGAGAGAGTATTCAAATCTGTTCAATTACACCAAAGATATATGCCAAACTAAAGGGGTAGGCAGCTCTGTAAATATGGAACATATAAAGAAGCATTATTATGGAAGCCATCCTACAATCAATCCATTTGGAATCATCCCTCTTGGCCCAAATATTGATTACTCTTCTCCTCATGATAGAGATAGATTTTCCTCATAG

mRNA sequence

ATGGCACGCTCAGCGCTCGATGAAATGTCTACAACAGGTGCTTTTGTAAGAACAGCTTCGACGTTTCGCAATTTGATTTCACGAGATGGATCCTCCCAGTTTCCATCAGAATCTGGGAGATACCATCTCTACATATCATATGCTTGTCCTTGGGCTTCCAGGTGCCTTGCCTATTTGAAAATCAAAGGACTCGAAAAAGCGATCAGTTTCACGTCAGTCAAACCAATATGGGAAAGAACCAAGGACTCTGATGATCATATGGGATGGGTTTTTCCTAGCTCTGATACAGAGGAGCCTGGTGCCGAACCTGACCCTTTGAATGGAGTTAGAAGTCTTAGAGAACTCTATGAACTTGCAAGTACAAATTACAGTGGGAAGTATACAGTTCCAGTTTTATGGGATATGAAACTCAAAACAATTGTTAACAATGAGAGCTCAGAGATAATTCGCATGCTTAATACTGAATTCAATGATATAGCTGAGAATCCATATCTGGATCTCTATCCCACTCACTTGCAAGCCCAAATTGATGAGACTAATGATTGGATATACCGTGGCATTAATAATGGTGTTTATAAATGTGGTTTTGCAAGGCAGCAACAGCCTTATGACCAGGCTGTGAAAGAGTTATATGAAGCTTTAGACAGATGTGAGGAAATACTTTCGAAGCAACGTTACCTCTGTGGGAACACACTGACTGAAGCAGACATTCGGCTGTTTGTCACCATTATAAGATTCGACGAGGTTTATGCAGTTCACTTCAAATGCAACAAGAAACTACTGAGAGAGTATTCAAATCTGTTCAATTACACCAAAGATATATGCCAAACTAAAGGGGTAGGCAGCTCTGTAAATATGGAACATATAAAGAAGCATTATTATGGAAGCCATCCTACAATCAATCCATTTGGAATCATCCCTCTTGGCCCAAATATTGATTACTCTTCTCCTCATGATAGAGATAGATTTTCCTCATAG

Coding sequence (CDS)

ATGGCACGCTCAGCGCTCGATGAAATGTCTACAACAGGTGCTTTTGTAAGAACAGCTTCGACGTTTCGCAATTTGATTTCACGAGATGGATCCTCCCAGTTTCCATCAGAATCTGGGAGATACCATCTCTACATATCATATGCTTGTCCTTGGGCTTCCAGGTGCCTTGCCTATTTGAAAATCAAAGGACTCGAAAAAGCGATCAGTTTCACGTCAGTCAAACCAATATGGGAAAGAACCAAGGACTCTGATGATCATATGGGATGGGTTTTTCCTAGCTCTGATACAGAGGAGCCTGGTGCCGAACCTGACCCTTTGAATGGAGTTAGAAGTCTTAGAGAACTCTATGAACTTGCAAGTACAAATTACAGTGGGAAGTATACAGTTCCAGTTTTATGGGATATGAAACTCAAAACAATTGTTAACAATGAGAGCTCAGAGATAATTCGCATGCTTAATACTGAATTCAATGATATAGCTGAGAATCCATATCTGGATCTCTATCCCACTCACTTGCAAGCCCAAATTGATGAGACTAATGATTGGATATACCGTGGCATTAATAATGGTGTTTATAAATGTGGTTTTGCAAGGCAGCAACAGCCTTATGACCAGGCTGTGAAAGAGTTATATGAAGCTTTAGACAGATGTGAGGAAATACTTTCGAAGCAACGTTACCTCTGTGGGAACACACTGACTGAAGCAGACATTCGGCTGTTTGTCACCATTATAAGATTCGACGAGGTTTATGCAGTTCACTTCAAATGCAACAAGAAACTACTGAGAGAGTATTCAAATCTGTTCAATTACACCAAAGATATATGCCAAACTAAAGGGGTAGGCAGCTCTGTAAATATGGAACATATAAAGAAGCATTATTATGGAAGCCATCCTACAATCAATCCATTTGGAATCATCCCTCTTGGCCCAAATATTGATTACTCTTCTCCTCATGATAGAGATAGATTTTCCTCATAG

Protein sequence

MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLKIKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELASTNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETNDWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLFVTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTINPFGIIPLGPNIDYSSPHDRDRFSS*
Homology
BLAST of Chy12G211070 vs. ExPASy Swiss-Prot
Match: P42620 (Glutathionyl-hydroquinone reductase YqjG OS=Escherichia coli (strain K12) OX=83333 GN=yqjG PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 9.8e-82
Identity = 160/321 (49.84%), Postives = 201/321 (62.62%), Query Frame = 0

Query: 7   DEMSTTGAFVRTASTFRNLISRD------GSSQFPSESGRYHLYISYACPWASRCLAYLK 66
           D  ST G F R+AS FRN ++ D      G+  F +E  RYHLY+S ACPWA R L   K
Sbjct: 15  DTKSTGGKFQRSASAFRNWLTADGAPGPTGTGGFIAEKDRYHLYVSLACPWAHRTLIMRK 74

Query: 67  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 126
           +KGLE  IS + V P+           GW F   D   PGA  D L     L +LY  A 
Sbjct: 75  LKGLEPFISVSVVNPLMLEN-------GWTF---DDSFPGATGDTLYQNEFLYQLYLHAD 134

Query: 127 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 186
            +YSG+ TVPVLWD K  TIV+NES+EIIRM NT F+ +      D YP  LQ +IDE N
Sbjct: 135 PHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAFDALGAKAG-DYYPPALQTKIDELN 194

Query: 187 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 246
            WIY  +NNGVYK GFA  Q+ YD+AV +++E+L R E+IL + RYL GN LTEADIRL+
Sbjct: 195 GWIYDTVNNGVYKAGFATSQEAYDEAVAKVFESLARLEQILGQHRYLTGNQLTEADIRLW 254

Query: 247 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 306
            T++RFD VY  HFKC+K  + +Y NL+ + +DI Q  G+  +VN +HI+ HY+ SH TI
Sbjct: 255 TTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRDIYQMPGIAETVNFDHIRNHYFRSHKTI 314

Query: 307 NPFGIIPLGPNIDYSSPHDRD 322
           NP GII +GP  D   PH RD
Sbjct: 315 NPTGIISIGPWQDLDEPHGRD 324

BLAST of Chy12G211070 vs. ExPASy Swiss-Prot
Match: Q8KN33 (Glutathionyl-hydroquinone reductase PcpF OS=Sphingobium chlorophenolicum OX=46429 GN=pcpF PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-66
Identity = 130/304 (42.76%), Postives = 179/304 (58.88%), Query Frame = 0

Query: 7   DEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLKIKGLEK 66
           D  S+ G FVR  S +R  +     + F  E GRYHLY  +ACPWA R L    +KGLE+
Sbjct: 15  DTKSSGGRFVRKESQYRGGL----DAGFRGEPGRYHLYAGFACPWAHRVLIMRALKGLEE 74

Query: 67  AISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELASTNYSGK 126
            IS + V              GW F   D       PD +NG   L ++Y  A   Y+G+
Sbjct: 75  MISVSMVNAYMGEN-------GWTFLPGD----DVVPDSINGADYLYQVYTAADPTYTGR 134

Query: 127 YTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETNDWIYRG 186
            T+P+LWD   K I+NNESSEIIR+LN+ F+D+   P  D YP   + +ID  N  +Y  
Sbjct: 135 VTIPILWDKVEKRILNNESSEIIRILNSAFDDVGALPG-DYYPAEFRPEIDRINARVYET 194

Query: 187 INNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLFVTIIRF 246
           +NNGVY+ GFA  Q+ Y++A   L++ LD  EE L+ + +L G+ LTEADIRLF T++RF
Sbjct: 195 LNNGVYRSGFATTQEAYEEAFYPLFDTLDWLEEHLTGREWLVGDRLTEADIRLFPTLVRF 254

Query: 247 DEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTINPFGII 306
           D +Y  HFKCN + + +Y NL      +   + V  ++N+ H K HYYGSHP++NP GI+
Sbjct: 255 DAIYHGHFKCNLRRIADYPNLSRLVGKLASHERVAPTINLRHAKAHYYGSHPSVNPTGIV 302

Query: 307 PLGP 311
           P+GP
Sbjct: 315 PVGP 302

BLAST of Chy12G211070 vs. ExPASy Swiss-Prot
Match: O94524 (Glutathione S-transferase omega-like 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gto2 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-63
Identity = 142/317 (44.79%), Postives = 187/317 (58.99%), Query Frame = 0

Query: 7   DEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLKIKGLEK 66
           D  S  G F R  S+FR  IS +    F  E  RYHLY+SYACPWA R L   K+KGLE 
Sbjct: 8   DWSSKDGEFRRQVSSFRERISPE-HKYFQPEKDRYHLYVSYACPWAHRTLIVRKLKGLEN 67

Query: 67  AISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELASTNYSGK 126
            I    V   W    +     GW F     +E  +  DPL     LR LY  A  NY+ +
Sbjct: 68  VIPVHVVG--WLMGPN-----GWNF----DKENDSTGDPLYNSPYLRNLYFRADPNYNMR 127

Query: 127 YTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP---YLDLYPTHLQAQIDETNDWI 186
           +TVPVLWD K  TIVNNES+EIIRM N  FN++ E+     +DLYP+ L+ +IDE ND+ 
Sbjct: 128 FTVPVLWDSKYNTIVNNESAEIIRMFNDAFNEVIEDEEKRVVDLYPSSLRTKIDELNDYF 187

Query: 187 YRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEIL--SKQRYLCGNTLTEADIRLFV 246
           Y  +NNGVYK GFA   + Y++ V+ +++ LDR E++L  SK  +L G+ LTE D+RL+ 
Sbjct: 188 YDTVNNGVYKTGFATTAEAYEKNVRVVFQGLDRLEQVLKESKGPFLLGDHLTETDVRLYT 247

Query: 247 TIIRFDEVYAVHFKCNKKLLR-EYSNLFNYTKDI-CQTKGVGSSVNMEHIKKHYYGSHPT 306
           TI+RFD VY  HFKCN   +R  Y ++  + K +  +      + + +HIK HY  SH  
Sbjct: 248 TIVRFDPVYVQHFKCNIGTIRHNYPHINQWLKRLYWKHPAFHETTDFKHIKCHYTQSHTQ 307

Query: 307 INPFGIIPLG--PNIDY 315
           INP GI PLG  PN++Y
Sbjct: 308 INPLGITPLGPIPNVEY 312

BLAST of Chy12G211070 vs. ExPASy Swiss-Prot
Match: P36156 (Glutathione S-transferase omega-like 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ECM4 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 136/359 (37.88%), Postives = 189/359 (52.65%), Query Frame = 0

Query: 11  TTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLKIKGL------ 70
           T GAF R  S+FR  IS+      P++ GRY LY+S ACPWA R L    +KGL      
Sbjct: 9   TNGAFKRQVSSFRETISKQHPIYKPAK-GRYWLYVSLACPWAHRTLITRALKGLTSVIGC 68

Query: 71  --------EKAISFTSVKPIWERTKDSDDHM-----GWVFPSSDTEEPGAE--------- 130
                   EK   F  ++   E ++D  +H      G      D+ +  AE         
Sbjct: 69  SVVHWHLDEKGWRFLDMEKQLEDSEDFLEHWHDVAGGIRTAKEDSSKSFAEIKNDSQRFM 128

Query: 131 ----PDPLNGVRSLRELYELASTNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTE-FN 190
                +P  G + + +LY  +   YS ++TVPVLWD++ +TIVNNESSEIIR+LN+  F+
Sbjct: 129 VDATNEPHYGYKRISDLYYKSDPQYSARFTVPVLWDLETQTIVNNESSEIIRILNSSAFD 188

Query: 191 DIAENPY--LDLYPTHLQAQIDETNDWIYRGINNGVYKCGFARQQQPYDQAVKELYEALD 250
           +  ++ +   DL P  L+ QID+ N W+Y  INNGVYK GFA + + Y+  V  ++E LD
Sbjct: 189 EFVDDDHKKTDLVPAQLKTQIDDFNSWVYDSINNGVYKTGFAEKAEVYESEVNNVFEHLD 248

Query: 251 RCEEILS------KQRY-------------LCGNTLTEADIRLFVTIIRFDEVYAVHFKC 310
           + E+ILS      K +Y               G+ LTEADIRL+ T+IRFD VY  HFKC
Sbjct: 249 KVEKILSDKYSKLKAKYGEEDRQKILGEFFTVGDQLTEADIRLYTTVIRFDPVYVQHFKC 308

Query: 311 NKKLLRE-YSNLFNYTKDI-CQTKGVGSSVNMEHIKKHYYGSHPTINPFGIIPLGPNID 314
           N   +R  Y  +  + +++         + + +HIK HY  SH  INP GI PLGP  D
Sbjct: 309 NFTSIRAGYPFIHLWVRNLYWNYDAFRYTTDFDHIKLHYTRSHTRINPLGITPLGPKPD 366

BLAST of Chy12G211070 vs. ExPASy Swiss-Prot
Match: Q04806 (Glutathione S-transferase omega-like 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GTO3 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.3e-49
Identity = 130/362 (35.91%), Postives = 179/362 (49.45%), Query Frame = 0

Query: 15  FVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLKIKGLEKAISFTSVK 74
           F R +S FR +IS D     P++ GRY LY++  CPWA R L    +KGL   I  +   
Sbjct: 13  FKRQSSPFREIISADHPIYKPAK-GRYWLYVALPCPWAQRTLITRALKGLAPIIGCSVAH 72

Query: 75  PIWERTKDSDDHMGWVF----------------------PSSDTEEPGAE---------- 134
             W       D  GW F                       + +T  P A           
Sbjct: 73  --WHL-----DDKGWRFLEEGDGKTNERHWFDIAGGISSVNLNTSTPVANIPNNAHRLLV 132

Query: 135 ---PDPLNGVRSLRELYELASTNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNT-EFND 194
               +P  G + L + Y     +Y G++TVPVLWD++  TIVNNESS+II ++N+  F++
Sbjct: 133 DGTDEPHYGYKRLSDFYFKTKPDYKGRFTVPVLWDLETCTIVNNESSDIIGIMNSAAFDE 192

Query: 195 IAENPY--LDLYPTHLQAQIDETNDWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDR 254
                Y  + L P  L+AQI E N W+Y  INNGVYK GFA   + Y++ V  L++ LD+
Sbjct: 193 FVGEEYRQVRLVPRSLEAQITEFNSWVYDKINNGVYKAGFAECAEVYEREVTSLFQYLDK 252

Query: 255 CEEILSKQ------------------RYLC-GNTLTEADIRLFVTIIRFDEVYAVHFKCN 314
            E +L K+                  RY   G+TLTEAD+RL+ TI+RFD VY  HFKCN
Sbjct: 253 LENLLDKKYTDLEAEYGKNNKDKILDRYFAIGDTLTEADVRLYPTIVRFDVVYHQHFKCN 312

Query: 315 KKLLR-EYSNLFNYTKDI-CQTKGVGSSVNMEHIKKHYYGSHPTINPFGIIPLGPNIDYS 318
              +R +YS +  + K+I  + +    + +  HIK  Y  S P +NP GI PLGP  D  
Sbjct: 313 LATIRDDYSRIHTWLKNIYWRHEAFQRTTDFTHIKLGYTRSQPRVNPIGITPLGPKPDIR 366

BLAST of Chy12G211070 vs. ExPASy TrEMBL
Match: A0A0A0M0U0 (GST C-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G701990 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.1e-188
Identity = 322/325 (99.08%), Postives = 324/325 (99.69%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK
Sbjct: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           +KGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS
Sbjct: 61  LKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP+LDLYPTHLQAQIDETN
Sbjct: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPHLDLYPTHLQAQIDETN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 326
           NPFGIIPLGPNIDYSSPHDRDRFSS
Sbjct: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325

BLAST of Chy12G211070 vs. ExPASy TrEMBL
Match: A0A1S3C9D6 (glutathionyl-hydroquinone reductase YqjG isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498268 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 5.8e-186
Identity = 319/325 (98.15%), Postives = 320/325 (98.46%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFP ESGRYHLYISYACPWASRCLAYLK
Sbjct: 11  MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPPESGRYHLYISYACPWASRCLAYLK 70

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRS+RELYELAS
Sbjct: 71  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSIRELYELAS 130

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP LDLYPTHLQAQID TN
Sbjct: 131 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPSLDLYPTHLQAQIDATN 190

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIY GINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 191 DWIYSGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 250

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 251 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 310

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 326
           NPFGIIPLGPNIDYSSPHDRDRFSS
Sbjct: 311 NPFGIIPLGPNIDYSSPHDRDRFSS 335

BLAST of Chy12G211070 vs. ExPASy TrEMBL
Match: A0A1S3C998 (glutathionyl-hydroquinone reductase YqjG isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498268 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 5.8e-186
Identity = 319/325 (98.15%), Postives = 320/325 (98.46%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFP ESGRYHLYISYACPWASRCLAYLK
Sbjct: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPPESGRYHLYISYACPWASRCLAYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRS+RELYELAS
Sbjct: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSIRELYELAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP LDLYPTHLQAQID TN
Sbjct: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPSLDLYPTHLQAQIDATN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIY GINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 181 DWIYSGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 326
           NPFGIIPLGPNIDYSSPHDRDRFSS
Sbjct: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325

BLAST of Chy12G211070 vs. ExPASy TrEMBL
Match: A0A5A7TK80 (Glutathionyl-hydroquinone reductase YqjG isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00150 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.4e-184
Identity = 316/322 (98.14%), Postives = 317/322 (98.45%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFP ESGRYHLYISYACPWASRCLAYLK
Sbjct: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPPESGRYHLYISYACPWASRCLAYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRS+RELYELAS
Sbjct: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSIRELYELAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP LDLYPTHLQAQID TN
Sbjct: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPSLDLYPTHLQAQIDATN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIY GINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 181 DWIYSGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDR 323
           NPFGIIPLGPNIDYSSPHDRDR
Sbjct: 301 NPFGIIPLGPNIDYSSPHDRDR 322

BLAST of Chy12G211070 vs. ExPASy TrEMBL
Match: A0A6J1JWZ5 (uncharacterized protein LOC111490438 OS=Cucurbita maxima OX=3661 GN=LOC111490438 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.2e-172
Identity = 295/325 (90.77%), Postives = 308/325 (94.77%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRD SSQFP+ESGRYHLYISYACPWASRCLAYLK
Sbjct: 31  MARSALDEMSTTGAFVRTASTFRNLISRDQSSQFPAESGRYHLYISYACPWASRCLAYLK 90

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGLEKAISF+SVKPIWERTKD+DDH GWVFP SDTEEPGAEPD LNG +S+R+LYELAS
Sbjct: 91  IKGLEKAISFSSVKPIWERTKDTDDHRGWVFPGSDTEEPGAEPDHLNGAKSVRDLYELAS 150

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           +NY+GKYTVPVLWD KLKTIVNNESSEIIRMLNTEFNDIAENP LDLYP HLQAQIDETN
Sbjct: 151 SNYTGKYTVPVLWDKKLKTIVNNESSEIIRMLNTEFNDIAENPALDLYPPHLQAQIDETN 210

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
            WIY  INNGVYKCGFAR QQPYDQAVKELYEALDRCE+ILSKQRYLCGNTL+EAD+RLF
Sbjct: 211 GWIYDAINNGVYKCGFARMQQPYDQAVKELYEALDRCEDILSKQRYLCGNTLSEADVRLF 270

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREY NLFNYTKDI QTKGV SSV+MEHIKKHYYGSHP I
Sbjct: 271 VTIIRFDEVYAVHFKCNKKLLREYPNLFNYTKDIYQTKGVSSSVHMEHIKKHYYGSHPQI 330

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 326
           NPFGIIPLGPNIDY+SPHDR RFSS
Sbjct: 331 NPFGIIPLGPNIDYTSPHDRHRFSS 355

BLAST of Chy12G211070 vs. NCBI nr
Match: XP_004145444.1 (uncharacterized protein LOC101218943 isoform X2 [Cucumis sativus] >KGN66874.1 hypothetical protein Csa_007043 [Cucumis sativus])

HSP 1 Score: 667 bits (1722), Expect = 3.31e-242
Identity = 322/325 (99.08%), Postives = 324/325 (99.69%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK
Sbjct: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           +KGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS
Sbjct: 61  LKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP+LDLYPTHLQAQIDETN
Sbjct: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPHLDLYPTHLQAQIDETN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325
           NPFGIIPLGPNIDYSSPHDRDRFSS
Sbjct: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325

BLAST of Chy12G211070 vs. NCBI nr
Match: XP_031736254.1 (uncharacterized protein LOC101218943 isoform X1 [Cucumis sativus])

HSP 1 Score: 667 bits (1722), Expect = 4.81e-242
Identity = 322/325 (99.08%), Postives = 324/325 (99.69%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK
Sbjct: 11  MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 70

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           +KGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS
Sbjct: 71  LKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 130

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP+LDLYPTHLQAQIDETN
Sbjct: 131 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPHLDLYPTHLQAQIDETN 190

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 191 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 250

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 251 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 310

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325
           NPFGIIPLGPNIDYSSPHDRDRFSS
Sbjct: 311 NPFGIIPLGPNIDYSSPHDRDRFSS 335

BLAST of Chy12G211070 vs. NCBI nr
Match: XP_008459045.1 (PREDICTED: glutathionyl-hydroquinone reductase YqjG isoform X2 [Cucumis melo])

HSP 1 Score: 659 bits (1701), Expect = 5.26e-239
Identity = 319/325 (98.15%), Postives = 320/325 (98.46%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFP ESGRYHLYISYACPWASRCLAYLK
Sbjct: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPPESGRYHLYISYACPWASRCLAYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRS+RELYELAS
Sbjct: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSIRELYELAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP LDLYPTHLQAQID TN
Sbjct: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPSLDLYPTHLQAQIDATN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIY GINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 181 DWIYSGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325
           NPFGIIPLGPNIDYSSPHDRDRFSS
Sbjct: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325

BLAST of Chy12G211070 vs. NCBI nr
Match: XP_008459044.1 (PREDICTED: glutathionyl-hydroquinone reductase YqjG isoform X1 [Cucumis melo])

HSP 1 Score: 659 bits (1701), Expect = 7.65e-239
Identity = 319/325 (98.15%), Postives = 320/325 (98.46%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFP ESGRYHLYISYACPWASRCLAYLK
Sbjct: 11  MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPPESGRYHLYISYACPWASRCLAYLK 70

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRS+RELYELAS
Sbjct: 71  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSIRELYELAS 130

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP LDLYPTHLQAQID TN
Sbjct: 131 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPSLDLYPTHLQAQIDATN 190

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIY GINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 191 DWIYSGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 250

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 251 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 310

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 325
           NPFGIIPLGPNIDYSSPHDRDRFSS
Sbjct: 311 NPFGIIPLGPNIDYSSPHDRDRFSS 335

BLAST of Chy12G211070 vs. NCBI nr
Match: KAA0043208.1 (glutathionyl-hydroquinone reductase YqjG isoform X1 [Cucumis melo var. makuwa] >TYK12260.1 glutathionyl-hydroquinone reductase YqjG isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 654 bits (1687), Expect = 3.44e-236
Identity = 316/322 (98.14%), Postives = 317/322 (98.45%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFP ESGRYHLYISYACPWASRCLAYLK
Sbjct: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPPESGRYHLYISYACPWASRCLAYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRS+RELYELAS
Sbjct: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSIRELYELAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
           TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENP LDLYPTHLQAQID TN
Sbjct: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPSLDLYPTHLQAQIDATN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           DWIY GINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF
Sbjct: 181 DWIYSGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDI QTKGVGSSVNMEHIKKHYYGSHPTI
Sbjct: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDIYQTKGVGSSVNMEHIKKHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDR 322
           NPFGIIPLGPNIDYSSPHDRDR
Sbjct: 301 NPFGIIPLGPNIDYSSPHDRDR 322

BLAST of Chy12G211070 vs. TAIR 10
Match: AT4G19880.1 (Glutathione S-transferase family protein )

HSP 1 Score: 557.4 bits (1435), Expect = 7.7e-159
Identity = 256/324 (79.01%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSA+DE S +GAF RTASTFRN +S+D +SQFP+ESGRYHLYISYACPWASRCL+YLK
Sbjct: 32  MARSAVDETSDSGAFQRTASTFRNFVSKDSNSQFPAESGRYHLYISYACPWASRCLSYLK 91

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGL+ AISF+SVKPIW RTK++D+HMGWVFP SDTE PGA+PD LNG +S+RELYE+AS
Sbjct: 92  IKGLDDAISFSSVKPIWGRTKETDEHMGWVFPGSDTEVPGADPDHLNGAKSVRELYEIAS 151

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
            NY+GKYTVPVLWD KLKT+VNNES+EIIRM NTEFN IA NP LDLYP+HLQA+IDETN
Sbjct: 152 PNYTGKYTVPVLWDKKLKTVVNNESAEIIRMFNTEFNHIAGNPDLDLYPSHLQAKIDETN 211

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           +WIY GINNGVY+CGFA++Q PY++AV+++YEALDRCEEIL K RY+CGNTLTE DIRLF
Sbjct: 212 EWIYNGINNGVYRCGFAKKQGPYEEAVEQVYEALDRCEEILGKHRYICGNTLTETDIRLF 271

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VT+IRFDEVYAVHFKCNKKL+REY NLFNYTKDI Q  G+ S+VNM HIK+HYYGSHP+I
Sbjct: 272 VTLIRFDEVYAVHFKCNKKLIREYPNLFNYTKDIFQIPGMSSTVNMNHIKQHYYGSHPSI 331

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFS 325
           NPFGIIP GPNIDY+SPHDR RFS
Sbjct: 332 NPFGIIPHGPNIDYTSPHDRHRFS 355

BLAST of Chy12G211070 vs. TAIR 10
Match: AT5G45020.1 (Glutathione S-transferase family protein )

HSP 1 Score: 548.5 bits (1412), Expect = 3.6e-156
Identity = 253/325 (77.85%), Postives = 289/325 (88.92%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARS +DE S +GAFVRTASTFRN +S+D  SQFP+ESGRYHLYISYACPWA RCL+YLK
Sbjct: 1   MARSGVDETSESGAFVRTASTFRNFVSQDPDSQFPAESGRYHLYISYACPWACRCLSYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGL++AI+F+SV  IW RTK++DDH GWVFP SDTE PGAEPD LNG +S+RELYE+AS
Sbjct: 61  IKGLDEAITFSSVHAIWGRTKETDDHRGWVFPDSDTELPGAEPDYLNGAKSVRELYEIAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
            NY GKYTVPVLWD KLKT+VNNESSEIIRM NTEFN IA+ P LDLYP+HL+  I+ETN
Sbjct: 121 PNYEGKYTVPVLWDKKLKTVVNNESSEIIRMFNTEFNGIAKTPSLDLYPSHLRDVINETN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
            W++ GINNGVYKCGFAR+Q+PY++AV +LYEA+DRCEE+L KQRY+CGNT TEADIRLF
Sbjct: 181 GWVFNGINNGVYKCGFARKQEPYNEAVNQLYEAVDRCEEVLGKQRYICGNTFTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VT+IRFDEVYAVHFKCNK+LLREY N+FNY KDI Q  G+ S+VNMEHIK+HYYGSHPTI
Sbjct: 241 VTLIRFDEVYAVHFKCNKRLLREYPNIFNYIKDIYQIHGMSSTVNMEHIKQHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 326
           NPFGIIP GPNIDYSSPHDRDRFSS
Sbjct: 301 NPFGIIPHGPNIDYSSPHDRDRFSS 325

BLAST of Chy12G211070 vs. TAIR 10
Match: AT5G45020.2 (Glutathione S-transferase family protein )

HSP 1 Score: 548.5 bits (1412), Expect = 3.6e-156
Identity = 253/325 (77.85%), Postives = 289/325 (88.92%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARS +DE S +GAFVRTASTFRN +S+D  SQFP+ESGRYHLYISYACPWA RCL+YLK
Sbjct: 1   MARSGVDETSESGAFVRTASTFRNFVSQDPDSQFPAESGRYHLYISYACPWACRCLSYLK 60

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGL++AI+F+SV  IW RTK++DDH GWVFP SDTE PGAEPD LNG +S+RELYE+AS
Sbjct: 61  IKGLDEAITFSSVHAIWGRTKETDDHRGWVFPDSDTELPGAEPDYLNGAKSVRELYEIAS 120

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
            NY GKYTVPVLWD KLKT+VNNESSEIIRM NTEFN IA+ P LDLYP+HL+  I+ETN
Sbjct: 121 PNYEGKYTVPVLWDKKLKTVVNNESSEIIRMFNTEFNGIAKTPSLDLYPSHLRDVINETN 180

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
            W++ GINNGVYKCGFAR+Q+PY++AV +LYEA+DRCEE+L KQRY+CGNT TEADIRLF
Sbjct: 181 GWVFNGINNGVYKCGFARKQEPYNEAVNQLYEAVDRCEEVLGKQRYICGNTFTEADIRLF 240

Query: 241 VTIIRFDEVYAVHFKCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTI 300
           VT+IRFDEVYAVHFKCNK+LLREY N+FNY KDI Q  G+ S+VNMEHIK+HYYGSHPTI
Sbjct: 241 VTLIRFDEVYAVHFKCNKRLLREYPNIFNYIKDIYQIHGMSSTVNMEHIKQHYYGSHPTI 300

Query: 301 NPFGIIPLGPNIDYSSPHDRDRFSS 326
           NPFGIIP GPNIDYSSPHDRDRFSS
Sbjct: 301 NPFGIIPHGPNIDYSSPHDRDRFSS 325

BLAST of Chy12G211070 vs. TAIR 10
Match: AT4G19880.2 (Glutathione S-transferase family protein )

HSP 1 Score: 543.1 bits (1398), Expect = 1.5e-154
Identity = 256/350 (73.14%), Postives = 294/350 (84.00%), Query Frame = 0

Query: 1   MARSALDEMSTTGAFVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLK 60
           MARSA+DE S +GAF RTASTFRN +S+D +SQFP+ESGRYHLYISYACPWASRCL+YLK
Sbjct: 32  MARSAVDETSDSGAFQRTASTFRNFVSKDSNSQFPAESGRYHLYISYACPWASRCLSYLK 91

Query: 61  IKGLEKAISFTSVKPIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELAS 120
           IKGL+ AISF+SVKPIW RTK++D+HMGWVFP SDTE PGA+PD LNG +S+RELYE+AS
Sbjct: 92  IKGLDDAISFSSVKPIWGRTKETDEHMGWVFPGSDTEVPGADPDHLNGAKSVRELYEIAS 151

Query: 121 TNYSGKYTVPVLWDMKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETN 180
            NY+GKYTVPVLWD KLKT+VNNES+EIIRM NTEFN IA NP LDLYP+HLQA+IDETN
Sbjct: 152 PNYTGKYTVPVLWDKKLKTVVNNESAEIIRMFNTEFNHIAGNPDLDLYPSHLQAKIDETN 211

Query: 181 DWIYRGINNGVYKCGFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLF 240
           +WIY GINNGVY+CGFA++Q PY++AV+++YEALDRCEEIL K RY+CGNTLTE DIRLF
Sbjct: 212 EWIYNGINNGVYRCGFAKKQGPYEEAVEQVYEALDRCEEILGKHRYICGNTLTETDIRLF 271

Query: 241 VTIIRFDE--------------------------VYAVHFKCNKKLLREYSNLFNYTKDI 300
           VT+IRFDE                          VYAVHFKCNKKL+REY NLFNYTKDI
Sbjct: 272 VTLIRFDEVSSYFQSKKKKYTICERISNVETLIQVYAVHFKCNKKLIREYPNLFNYTKDI 331

Query: 301 CQTKGVGSSVNMEHIKKHYYGSHPTINPFGIIPLGPNIDYSSPHDRDRFS 325
            Q  G+ S+VNM HIK+HYYGSHP+INPFGIIP GPNIDY+SPHDR RFS
Sbjct: 332 FQIPGMSSTVNMNHIKQHYYGSHPSINPFGIIPHGPNIDYTSPHDRHRFS 381

BLAST of Chy12G211070 vs. TAIR 10
Match: AT5G44990.1 (Glutathione S-transferase family protein )

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-138
Identity = 227/310 (73.23%), Postives = 268/310 (86.45%), Query Frame = 0

Query: 15  FVRTASTFRNLISRDGSSQFPSESGRYHLYISYACPWASRCLAYLKIKGLEKAISFTSVK 74
           F RTA++FRN +S+D  SQFP+ESGRYHLYISYACPWASRCLA LK+KGL+KAISF+SV+
Sbjct: 12  FARTATSFRNFVSKDPDSQFPAESGRYHLYISYACPWASRCLAILKLKGLDKAISFSSVQ 71

Query: 75  PIWERTKDSDDHMGWVFPSSDTEEPGAEPDPLNGVRSLRELYELASTNYSGKYTVPVLWD 134
           P+W  TK++D+HMGWVFP SDTE  GAE D +NG +S+RELY++AS+NY+GKYTVPVLWD
Sbjct: 72  PLWRNTKENDEHMGWVFPDSDTEVLGAERDHINGAKSVRELYDIASSNYTGKYTVPVLWD 131

Query: 135 MKLKTIVNNESSEIIRMLNTEFNDIAENPYLDLYPTHLQAQIDETNDWIYRGINNGVYKC 194
            KLKTIVNNESSEI+RM NTEFN +AENP LDLYP +L+A IDETN+WI+ GINNGVYKC
Sbjct: 132 KKLKTIVNNESSEILRMFNTEFNHVAENPSLDLYPPNLRAIIDETNEWIHDGINNGVYKC 191

Query: 195 GFARQQQPYDQAVKELYEALDRCEEILSKQRYLCGNTLTEADIRLFVTIIRFDEVYAVHF 254
           GFA  Q+ YD  VK LYEALDRCE+IL KQR+LCGNTLTE+DIRLFVT+IRFDE YAV F
Sbjct: 192 GFATNQETYDVEVKRLYEALDRCEDILRKQRFLCGNTLTESDIRLFVTVIRFDEAYAVIF 251

Query: 255 KCNKKLLREYSNLFNYTKDICQTKGVGSSVNMEHIKKHYYGSHPTINPFGIIPLGPNIDY 314
           KC+K+L+REY +LFNYTKDI Q  G+ S+V M+HIK++YYGS P+INP  II  GPNIDY
Sbjct: 252 KCDKRLVREYYHLFNYTKDIYQIAGMSSTVKMDHIKQNYYGSFPSINPLEIIAHGPNIDY 311

Query: 315 SSPHDRDRFS 325
           S PHDR RFS
Sbjct: 312 SLPHDRHRFS 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P426209.8e-8249.84Glutathionyl-hydroquinone reductase YqjG OS=Escherichia coli (strain K12) OX=833... [more]
Q8KN331.2e-6642.76Glutathionyl-hydroquinone reductase PcpF OS=Sphingobium chlorophenolicum OX=4642... [more]
O945241.6e-6344.79Glutathione S-transferase omega-like 2 OS=Schizosaccharomyces pombe (strain 972 ... [more]
P361561.1e-5337.88Glutathione S-transferase omega-like 2 OS=Saccharomyces cerevisiae (strain ATCC ... [more]
Q048068.3e-4935.91Glutathione S-transferase omega-like 3 OS=Saccharomyces cerevisiae (strain ATCC ... [more]
Match NameE-valueIdentityDescription
A0A0A0M0U02.1e-18899.08GST C-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G701... [more]
A0A1S3C9D65.8e-18698.15glutathionyl-hydroquinone reductase YqjG isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
A0A1S3C9985.8e-18698.15glutathionyl-hydroquinone reductase YqjG isoform X2 OS=Cucumis melo OX=3656 GN=L... [more]
A0A5A7TK802.4e-18498.14Glutathionyl-hydroquinone reductase YqjG isoform X1 OS=Cucumis melo var. makuwa ... [more]
A0A6J1JWZ51.2e-17290.77uncharacterized protein LOC111490438 OS=Cucurbita maxima OX=3661 GN=LOC111490438... [more]
Match NameE-valueIdentityDescription
XP_004145444.13.31e-24299.08uncharacterized protein LOC101218943 isoform X2 [Cucumis sativus] >KGN66874.1 hy... [more]
XP_031736254.14.81e-24299.08uncharacterized protein LOC101218943 isoform X1 [Cucumis sativus][more]
XP_008459045.15.26e-23998.15PREDICTED: glutathionyl-hydroquinone reductase YqjG isoform X2 [Cucumis melo][more]
XP_008459044.17.65e-23998.15PREDICTED: glutathionyl-hydroquinone reductase YqjG isoform X1 [Cucumis melo][more]
KAA0043208.13.44e-23698.14glutathionyl-hydroquinone reductase YqjG isoform X1 [Cucumis melo var. makuwa] >... [more]
Match NameE-valueIdentityDescription
AT4G19880.17.7e-15979.01Glutathione S-transferase family protein [more]
AT5G45020.13.6e-15677.85Glutathione S-transferase family protein [more]
AT5G45020.23.6e-15677.85Glutathione S-transferase family protein [more]
AT4G19880.21.5e-15473.14Glutathione S-transferase family protein [more]
AT5G44990.11.5e-13873.23Glutathione S-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 320..325
NoneNo IPR availableGENE3D1.20.1050.10coord: 176..325
e-value: 2.1E-70
score: 237.9
NoneNo IPR availablePFAMPF13410GST_C_2coord: 203..270
e-value: 4.4E-9
score: 36.2
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 2..175
e-value: 5.0E-65
score: 220.2
NoneNo IPR availablePANTHERPTHR32419:SF29SUBFAMILY NOT NAMEDcoord: 1..324
NoneNo IPR availableCDDcd03190GST_C_Omega_likecoord: 169..310
e-value: 8.12432E-87
score: 255.191
IPR004045Glutathione S-transferase, N-terminalPFAMPF13409GST_N_2coord: 48..155
e-value: 8.0E-19
score: 67.7
IPR016639Glutathione S-transferase Omega/GSHPIRSFPIRSF015753GSTcoord: 1..321
e-value: 2.7E-129
score: 428.6
IPR016639Glutathione S-transferase Omega/GSHSFLDSFLDG01206Xi.1coord: 7..323
e-value: 0.0
score: 422.1
IPR016639Glutathione S-transferase Omega/GSHPANTHERPTHR32419GLUTATHIONYL-HYDROQUINONE REDUCTASEcoord: 1..324
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 7..323
e-value: 0.0
score: 422.1
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 169..293
score: 12.648208
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 162..299
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 23..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G211070.1Chy12G211070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding