Chy12G210900 (gene) Cucumber (hystrix) v1

Overview
NameChy12G210900
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionE3 ubiquitin-protein ligase arih1
LocationchrH12: 6504973 .. 6508869 (+)
RNA-Seq ExpressionChy12G210900
SyntenyChy12G210900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCATCGTGAGATTTTTGGTCGTGGTTCTTCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTCCGAAGTTGTTGTGAAGATCACGAAGTGTGGAAAGAGACTGAAGATGCAGTAGAAGAAGAGTCTGAGGAGGATCTTGATGAGTTTTCTGTGAAAATGTTCTTTAAGGGCGTTTCAATAGCTGAGATTGGGGACCCTGCTTCTGGTCTTTCTGGTATTGGAGTATTAATGGAAAAATCAGGGAAAATCCCTTTTGTTCAGGTTCAGAAAAAACTTGATTTTTATGTTGACGAGTTAGTTGCTGATTATCTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGATGTCCGCTGTATTCGAGCTTTCACCAATTCTGATCTGTTATATAAACAGGTTATTACTTTCCCAACCTCCTAAATTTTGCTTGTTATGATAGATGCTAACTATCAGTTTTTTTCTTCATGCTCCTACTGGAAGTTTGTTGTATTTTTCTAACTGCATTTTTGGGGTATGTTTGTATTGTTTTTCAAAAGAAAAATTGTGAATTCTCACTTACTAATCTAGTGATGTAATTTTCTGGAAATTCTAGTTTTTTTTGTTTTAAATTTGACTAGTTCTATATTATCTTCTTTATGAGAATCAAATTTTATTGATGACACGAAATTAGTCAAGAGAGTGGAGGGGTTAGGATTGATTATTACCATTCTTCTGCAGAATCCAGAGAAGGATAGGGTTAGTTTCATTTTTCAGGGCAAGTCATTTTGAGTGGGTTAGGAGTGACTTTATGAGCTGTGGAAGTCGAGGAGAGTGGAGAGATGCATGAACTCTTATAGTTTTTTAGCCATTAGCTTACGAGTTTCTTATATGCTCAGATAATATGAATGTTTGACAGATAACAAATCAAGAGGAGCCTGAAATTCCACTTCTTGTTGCTCTGAAAGAAAGGATTCTGGAACATGCTAGCAATTTTGAATCCTTTTCCCTGAGCCTTTCTTCAAGTGCTGATCTTCAGCAGCCATTTCATCTTGCCAAAGTGGCTATTGGTGTTCTTTCTTGTCCGCAAAAAGGAGATAAATCGGTTGAAAATTGTTCTATTTGTTGTGACAATAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCGCAATGTATGAAGACTTATGTTGATGGAAAAGTAGATGCATCTCAAGTTCCTATCAGATGCCCTCAGTTGAGATGCAAATATTACATCACAAGCAATGAGTTCAAATCTTTTCTTCCCCTGACTTTATATGAATCATTAGAGAATACCTTAGCAGAAGCAAATATTCATGCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCTTGTTAGACCCCAGCGAATGTTTGTCAGCTAGGGCAAGTTCATCAAGTCAATCAGAAAATAGCTGCATTGAGTGTCCAGTATGTCAGAGATTTATCTGTGTTGAATGTCAAGTTCCTTGGCATTCATCAGTGAGCTGCGAAGAATTTCAGAACATCCCATTAGAGGAGAGAGACACTGCAGATATTACCTTACATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGGAGTGTCATAGGATGATTGAATTAACTCAGGGTTGCTTTCATATGACATGCTGGTAAGGCTACTTCCTCCAAATCTCACTTTATTTATGGTAATCATTTTGTTTGTACATATTGTGGGAAAATCGGTGATCGAGCATCAATATAAATAGATATCAGACACTTACTGGGATGTTAGATTTCTGAAGGATTGGGTTGTAGGACTTGTTGAGAAACTAAAACTTAGATGCACTTTATATGTTTGTGGATAATCAAAAGGCATGCATACAAAAAAAATCTTCCTTCCTCTTTCTAATATCTTTGCTTTCTGCTAACCATCATGGGTTGACATAATTGTAAAAGAGGAGACATGGTCTCAATAACTAACTAAGAGATCATGGGTTCAATCCATGGTGATCACTTACCTAGAGTTAATTTTCTACAAGTTTCTTTGATATCTAAATGCTGTAGGGTCAGACGAATTGTCCTGTGAGGTTAGTCGAGGAACTCGTAAGCTGACCTGGACACTCATGGATTTCAAAAAAAAAAAAATCTTTGCTTTATGCTAGATTAGATTTTTTATTGTTTTACAAATTTTCCAGCTGCAAATTGTTTACTTATCGGGTCCATCTCTCTCGGGAACTGCTGTTTATCACAAACTTTACCAGTTTACATGAATCCATATTTCTAAATGTCCTATTGGGATTTGTTGCAAACATAAATTCAAACTTTTCACATCAATGCTACCAATCCCAAGTTATTGAAATTGTGAGGCAAATGTTTCCATAAGGTTTTACCGTTATGTGAATACAATGCCTATATACTAGGGAAAATATTAATTTGGTGCATGTGTTATATCAGCCTTATTGTTCAATGTGATGAACATGGTTGATTTCATAACTTCACTAGTATATCTATCTTATTTGAAGAAATAGAATGTGGGTAAGCACATAATAGTAAGCATGTTAAATGTCATTTGCCACTATATATTGGTAGTCTGAGCTGGTCATACAATGATCACAGCCTTTTTAGGGATTTCTCTAGAGGTGGAACACCTCAACCAGTCCACTTAAACTAATGTCGGTGTGATGATAGTGGTAAAAGAGTGATCAATGAATATAAGGTCAAATTTACAGGTCTCTGCTTATCTAGAAATGTTTTCGACTCCTTGGGTGTTAAATATGATGAAACGGTATATTATTATTTTTTTTTTATTGCTTTTGCTTTTGCTATGTTTACTACTTCCTGTCTATTTCCTTTAATCTCGCATTGAGACTCAGGCAAGAGTCTAGTCTTTCAAGCACACTATTATTTACATCAATGAACATATGTGCTCACTGTATTCTTGTTTTCACTCCCACATGGCGACTATATGTTAGTCACTATGATATTAACTTTGCAGATATAAGAATTGTTTGGTGTGTGATGCTTTATTGAGACCTTGCAGATAAAAAGATTGGTCCTTTATAGCTTTATTGGTAAATATAATTTGTTATGCAATTGTTTCAAGAAATAACTTGAATGCATCTCCTGCTTCTTCTGAACCAAATCTGCCCATAAAACACTCATCTCTTTCTACCAAGCTAGAATTGTCATCCTAATTTAACTGCACACATTTCTCCAGTAAAAGTTGAATTCCCAAATGGCCTTTATTCCAAACCCATTGGTTTACGTTCTTTCTTCATTTAGTGACTACTATTCCTGAGTAGGTGTTTGGGAAATTGAGATTTTCAAACATGTATGAACTATCTCAGATTTATACATCTCTATGGCCAGGCAAATGGGATTTAATATTTGCACTCAAAAGTTTTATGAAAATGATCCACAAGTTGGCAATATCCGGTTTTGTCTTAAGCTGGTTTGTGCAGGGCTTAATGTATATTACTTAGGTTGGATGCTTATACTGCTTTGCTTGGTGTGCTTACAGTTGTGGGCATGAATTCTGCTATTCTTGTGGTGTTGAGTATCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGAAGATAATTCCGAGAACCTGGTCACCCACTCCGTCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGATGCATACTCAGAGCAAGAGAGGTCACAATTGGCACTAATTCAACGATTCCTCGCTGGGGGATTTAGTCTGAGCGACCACCAACCATATCAGTCTCCACCACGATGTACAGACTCTTATGTCGACACTATGAAGGACATACACCAGCTTCCTTGGCTTGAAAGGTTTGTTTCTGTTATAAGCGATAGCTACTATGAAGATTATGTACAATGA

mRNA sequence

ATGGAGCATCGTGAGATTTTTGGTCGTGGTTCTTCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTCCGAAGTTGTTGTGAAGATCACGAAGTGTGGAAAGAGACTGAAGATGCAGTAGAAGAAGAGTCTGAGGAGGATCTTGATGAGTTTTCTGTGAAAATGTTCTTTAAGGGCGTTTCAATAGCTGAGATTGGGGACCCTGCTTCTGGTCTTTCTGGTATTGGAGTATTAATGGAAAAATCAGGGAAAATCCCTTTTGTTCAGGTTCAGAAAAAACTTGATTTTTATGTTGACGAGTTAGTTGCTGATTATCTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGATGTCCGCTGTATTCGAGCTTTCACCAATTCTGATCTGTTATATAAACAGATAACAAATCAAGAGGAGCCTGAAATTCCACTTCTTGTTGCTCTGAAAGAAAGGATTCTGGAACATGCTAGCAATTTTGAATCCTTTTCCCTGAGCCTTTCTTCAAGTGCTGATCTTCAGCAGCCATTTCATCTTGCCAAAGTGGCTATTGGTGTTCTTTCTTGTCCGCAAAAAGGAGATAAATCGGTTGAAAATTGTTCTATTTGTTGTGACAATAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCGCAATGTATGAAGACTTATGTTGATGGAAAAGTAGATGCATCTCAAGTTCCTATCAGATGCCCTCAGTTGAGATGCAAATATTACATCACAAGCAATGAGTTCAAATCTTTTCTTCCCCTGACTTTATATGAATCATTAGAGAATACCTTAGCAGAAGCAAATATTCATGCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCTTGTTAGACCCCAGCGAATGTTTGTCAGCTAGGGCAAGTTCATCAAGTCAATCAGAAAATAGCTGCATTGAGTGTCCAGTATGTCAGAGATTTATCTGTGTTGAATGTCAAGTTCCTTGGCATTCATCAGTGAGCTGCGAAGAATTTCAGAACATCCCATTAGAGGAGAGAGACACTGCAGATATTACCTTACATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGGAGTGTCATAGGATGATTGAATTAACTCAGGGTTGCTTTCATATGACATGCTGTTGTGGGCATGAATTCTGCTATTCTTGTGGTGTTGAGTATCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGAAGATAATTCCGAGAACCTGGTCACCCACTCCGTCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGATGCATACTCAGAGCAAGAGAGGTCACAATTGGCACTAATTCAACGATTCCTCGCTGGGGGATTTAGTCTGAGCGACCACCAACCATATCAGTCTCCACCACGATGTACAGACTCTTATGTCGACACTATGAAGGACATACACCAGCTTCCTTGGCTTGAAAGGTTTGTTTCTGTTATAAGCGATAGCTACTATGAAGATTATGTACAATGA

Coding sequence (CDS)

ATGGAGCATCGTGAGATTTTTGGTCGTGGTTCTTCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTCCGAAGTTGTTGTGAAGATCACGAAGTGTGGAAAGAGACTGAAGATGCAGTAGAAGAAGAGTCTGAGGAGGATCTTGATGAGTTTTCTGTGAAAATGTTCTTTAAGGGCGTTTCAATAGCTGAGATTGGGGACCCTGCTTCTGGTCTTTCTGGTATTGGAGTATTAATGGAAAAATCAGGGAAAATCCCTTTTGTTCAGGTTCAGAAAAAACTTGATTTTTATGTTGACGAGTTAGTTGCTGATTATCTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGATGTCCGCTGTATTCGAGCTTTCACCAATTCTGATCTGTTATATAAACAGATAACAAATCAAGAGGAGCCTGAAATTCCACTTCTTGTTGCTCTGAAAGAAAGGATTCTGGAACATGCTAGCAATTTTGAATCCTTTTCCCTGAGCCTTTCTTCAAGTGCTGATCTTCAGCAGCCATTTCATCTTGCCAAAGTGGCTATTGGTGTTCTTTCTTGTCCGCAAAAAGGAGATAAATCGGTTGAAAATTGTTCTATTTGTTGTGACAATAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCGCAATGTATGAAGACTTATGTTGATGGAAAAGTAGATGCATCTCAAGTTCCTATCAGATGCCCTCAGTTGAGATGCAAATATTACATCACAAGCAATGAGTTCAAATCTTTTCTTCCCCTGACTTTATATGAATCATTAGAGAATACCTTAGCAGAAGCAAATATTCATGCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCTTGTTAGACCCCAGCGAATGTTTGTCAGCTAGGGCAAGTTCATCAAGTCAATCAGAAAATAGCTGCATTGAGTGTCCAGTATGTCAGAGATTTATCTGTGTTGAATGTCAAGTTCCTTGGCATTCATCAGTGAGCTGCGAAGAATTTCAGAACATCCCATTAGAGGAGAGAGACACTGCAGATATTACCTTACATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGGAGTGTCATAGGATGATTGAATTAACTCAGGGTTGCTTTCATATGACATGCTGTTGTGGGCATGAATTCTGCTATTCTTGTGGTGTTGAGTATCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGAAGATAATTCCGAGAACCTGGTCACCCACTCCGTCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGATGCATACTCAGAGCAAGAGAGGTCACAATTGGCACTAATTCAACGATTCCTCGCTGGGGGATTTAGTCTGAGCGACCACCAACCATATCAGTCTCCACCACGATGTACAGACTCTTATGTCGACACTATGAAGGACATACACCAGCTTCCTTGGCTTGAAAGGTTTGTTTCTGTTATAAGCGATAGCTACTATGAAGATTATGTACAATGA

Protein sequence

MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKGVSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQNDVRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ*
Homology
BLAST of Chy12G210900 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 2.2e-19
Identity = 63/204 (30.88%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 202 CSICCDNKPSAMMVALKCCHK-FCSQCMKTYVDGKVDASQVPIRCPQLRCKYYITSNEFK 261
           C +C ++KP   +  L CC K  C +C+K Y+  +V   QV I+CP   C  ++   E  
Sbjct: 236 CRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFL--EETT 295

Query: 262 SFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSECLSARASSSSQSENSCIECP 321
               LT  +S++          D    P P C       +       S S+S    I+CP
Sbjct: 296 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYK-IQCP 355

Query: 322 VCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRWRRCQECHRMIELT 381
            CQ   C +C  PWH  V+C+E++      R  A    H     +  ++C +C   I+ T
Sbjct: 356 TCQLIWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 415

Query: 382 QGCFHMTCC-CGHEFCYSCGVEYQ 404
           +GC HMTC  C   FCY CG  Y+
Sbjct: 416 EGCDHMTCSQCNTNFCYRCGERYR 430

BLAST of Chy12G210900 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 98.2 bits (243), Expect = 2.9e-19
Identity = 63/204 (30.88%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 202 CSICCDNKPSAMMVALKCCHK-FCSQCMKTYVDGKVDASQVPIRCPQLRCKYYITSNEFK 261
           C +C ++KP   +  L CC K  C +C+K Y+  +V   QV I+CP   C  ++   E  
Sbjct: 263 CRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFL--EETT 322

Query: 262 SFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSECLSARASSSSQSENSCIECP 321
               LT  +S++          D    P P C       +       S S+S+   I+CP
Sbjct: 323 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYK-IQCP 382

Query: 322 VCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRWRRCQECHRMIELT 381
            CQ   C +C  PWH  V+C+E++      R  A    H     +  ++C +C   I+ T
Sbjct: 383 TCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 442

Query: 382 QGCFHMTCC-CGHEFCYSCGVEYQ 404
           +GC HMTC  C   FCY CG  Y+
Sbjct: 443 EGCDHMTCSQCNTNFCYRCGERYR 457

BLAST of Chy12G210900 vs. ExPASy Swiss-Prot
Match: Q4KLT0 (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.7e-17
Identity = 59/206 (28.64%), Postives = 90/206 (43.69%), Query Frame = 0

Query: 201 NCSICCDNKPSAMMVALKCCHK-FCSQCMKTYVDGKVDASQVPIRCPQLRCKYYITSNEF 260
           +C +C +++    +  L CC K  C +C+K Y+  +V   Q  I+CP   C  ++  +  
Sbjct: 2   SCRVCLEDR---SIKPLPCCKKPVCDECLKRYLSSQVQLGQAEIQCPITECNKHLDESTI 61

Query: 261 KSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSECLSARASSSSQSENSC-IE 320
              LP       +  L  + +  D    P P C               + ++SEN   I+
Sbjct: 62  LYSLPHDDIIKYKYFLELSRM--DSSTKPCPQCKHFTTFKR--KTHIPNPTKSENKLKIQ 121

Query: 321 CPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRWRRCQECHRMIE 380
           CP CQ   C  C  PWH  V+C E++      R  A+   H     +  ++C  C   I+
Sbjct: 122 CPSCQFIWCFRCHAPWHEGVNCREYKKGDKLLRHWANEIEH---GQRNAQKCPRCKVHIQ 181

Query: 381 LTQGCFHMTCC-CGHEFCYSCGVEYQ 404
            T+GC HMTC  C   FCY CG  Y+
Sbjct: 182 RTEGCDHMTCSQCNTNFCYRCGERYR 197

BLAST of Chy12G210900 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 5.6e-15
Identity = 55/207 (26.57%), Postives = 86/207 (41.55%), Query Frame = 0

Query: 201 NCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ--VPIRCPQLRCKYYITSNE 260
           +C IC ++ P     AL C H++C  C K Y++ KV      +   CP  +CK  +  + 
Sbjct: 138 SCLICLEDYPPTQTFALICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 197

Query: 261 FKSFLPLTLYESLENTLAEANI--HADRIYCPFPNCSVLLDPSECLSARASSSSQSENSC 320
           FK  +   ++E   N + ++ +  +    +CP P C          S R     + E   
Sbjct: 198 FKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGC--------IYSIRCDRKERKEAVN 257

Query: 321 IECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRWRRCQECHRM 380
            +C     F C + ++  H    C +      +  D ++     LA  K   +C EC   
Sbjct: 258 CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCPECRSP 317

Query: 381 IELTQGCFHMTC-----CCGHEFCYSC 399
           IE   GC HMTC      CG EFC+ C
Sbjct: 318 IEKNGGCMHMTCRKNAGGCGFEFCWLC 333

BLAST of Chy12G210900 vs. ExPASy Swiss-Prot
Match: A2VEA3 (E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 8.9e-13
Identity = 62/230 (26.96%), Postives = 93/230 (40.43%), Query Frame = 0

Query: 202 CSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKV--DASQVPIRCPQLRCKYYITSNEF 261
           C IC  N P++    L+C HKFC QC   Y+  K+  +     I CP   C   +  N  
Sbjct: 184 CQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTV 243

Query: 262 -----KSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSECLSARASSSSQSEN 321
                 S + L     + N+  E N      +CP P+C  ++                + 
Sbjct: 244 MRLITDSKVKLKYQHLITNSFVECNRLLK--WCPAPDCHHVV-----------KVQYPDA 303

Query: 322 SCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRWRRCQECH 381
             + C  C R  C  C   WH  V C+  +   +++ D    T + +A N   + C +CH
Sbjct: 304 KPVRCK-CGRQFCFNCGENWHDPVKCKWLKKW-IKKCDDDSETSNWIAANT--KECPKCH 363

Query: 382 RMIELTQGCFHMTC---CCGHEFCYSC-GVEYQDGQQTCQCAFWDEDNSE 421
             IE   GC HM C    C  EFC+ C G     G     C  ++ED+++
Sbjct: 364 VTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAK 396

BLAST of Chy12G210900 vs. ExPASy TrEMBL
Match: A0A0A0M0W5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G706610 PE=3 SV=1)

HSP 1 Score: 1049.7 bits (2713), Expect = 4.1e-303
Identity = 509/515 (98.83%), Postives = 510/515 (99.03%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60
           MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120
           VSIAEIGD ASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND
Sbjct: 61  VSIAEIGDSASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFH 180
           VRCIRAFT+SDLLYKQITN EEPEIPLLVALKERILEHASNFESFSLSLSS ADLQQPFH
Sbjct: 121 VRCIRAFTSSDLLYKQITNHEEPEIPLLVALKERILEHASNFESFSLSLSSGADLQQPFH 180

Query: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240
           LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360
           CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR
Sbjct: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360

Query: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420
           LAQNKRWRRCQECHRMIELTQGCFHMTC CGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE
Sbjct: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420

Query: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480
           NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP QSPPR
Sbjct: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPCQSPPR 480

Query: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 516
           CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of Chy12G210900 vs. ExPASy TrEMBL
Match: A0A1S3C9E4 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.5e-302
Identity = 504/515 (97.86%), Postives = 511/515 (99.22%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60
           MEHR+IFGRGSSHGPAEEDDVE+FRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120
           VSIAEIGDPASGLSGIGV MEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND
Sbjct: 61  VSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFH 180
           VRCIRAFT+SDLLYKQITN+EEPEIPLLVALKERILEHASNFESFSL LSSSADL+QPFH
Sbjct: 121 VRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPFH 180

Query: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240
           LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQC+KTYVDGKVDASQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYE LENTLAEANIHADRIYCPFPNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360
           CLSARASSSSQS+NSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR
Sbjct: 301 CLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360

Query: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420
           LAQNKRWRRCQECHRMIELTQGCFHMTC CGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE
Sbjct: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420

Query: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480
           NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR
Sbjct: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480

Query: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 516
           CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of Chy12G210900 vs. ExPASy TrEMBL
Match: A0A5D3E1X1 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G001170 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.5e-302
Identity = 504/515 (97.86%), Postives = 511/515 (99.22%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60
           MEHR+IFGRGSSHGPAEEDDVE+FRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120
           VSIAEIGDPASGLSGIGV MEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND
Sbjct: 61  VSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFH 180
           VRCIRAFT+SDLLYKQITN+EEPEIPLLVALKERILEHASNFESFSL LSSSADL+QPFH
Sbjct: 121 VRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPFH 180

Query: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240
           LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQC+KTYVDGKVDASQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYE LENTLAEANIHADRIYCPFPNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360
           CLSARASSSSQS+NSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR
Sbjct: 301 CLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360

Query: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420
           LAQNKRWRRCQECHRMIELTQGCFHMTC CGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE
Sbjct: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420

Query: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480
           NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR
Sbjct: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480

Query: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 516
           CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of Chy12G210900 vs. ExPASy TrEMBL
Match: A0A6J1CLL9 (uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012584 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.2e-278
Identity = 463/517 (89.56%), Postives = 488/517 (94.39%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEE--ESEEDLDEFSVKMFF 60
           MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKE ED VEE  E EE LDEFSVKMFF
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGSEEEEHLDEFSVKMFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KG+SIAEIGDPASGLSGIGV MEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDVRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQP 180
           N++RCIRAFT+SDLLY QITN+EE EIPLL+ALKERILEHAS FE+FSL+LSSS DL+QP
Sbjct: 121 NEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDA 240
           FHLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMV LKCCHKFCS CMKTYVDGKV +
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGKVQS 240

Query: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYIT NEF+SFLPLTLYESLENTLAEANIH+DRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360
           SECLS RASSSSQS+NSCIECPVCQRFICV C VPWHSS+SCEEFQNI     DT DITL
Sbjct: 301 SECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTEDITL 360

Query: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDN 420
           HRLAQNKRW+RCQ+C RMIELTQGC+HMTC CGHEFCYSCG EYQD QQTCQCA WDE+N
Sbjct: 361 HRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALWDEEN 420

Query: 421 SENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP+QSP
Sbjct: 421 SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPHQSP 480

Query: 481 PRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 516
           PRCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

BLAST of Chy12G210900 vs. ExPASy TrEMBL
Match: A0A6J1EGQ6 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434167 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.4e-273
Identity = 455/518 (87.84%), Postives = 490/518 (94.59%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEES--EEDLDEFSVKMFF 60
           M+HREIFGRGSS   AE+DD EDFRSCCEDHEVWK+ E A EE+S  EEDLDEFSVK+FF
Sbjct: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIA IGDPASGLSGIGV MEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDVRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQP 180
           ND+  IRAFT+SD LYKQITN+E+PEIPLL+ALKERILEHASNFE+FSL+LSSS DL+QP
Sbjct: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDA 240
           FHLAKVAIGVLS P+KGDKSVENCSICCDNKPSAM+VALKCCHKFCS C+KTYVDGKV+A
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYIT+NEF+SFLPLTLYESL+NTLAEAN+ +DRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360
           SECLSARASSSSQS+NSC ECPVCQRFICVECQVPWHSS+SCEEFQN+PLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360

Query: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDN 420
             LAQNKRWRRCQEC RMIELTQGC+HMTC CGHEFCYSCG EYQDGQQTC+C  WDE+N
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420

Query: 421 SENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE++VTHS+QESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTD-SYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 516
           PRCTD SYVDTMKDIHQLPWLERFVS+ISDSYYEDYVQ
Sbjct: 481 PRCTDSSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

BLAST of Chy12G210900 vs. NCBI nr
Match: XP_004145453.1 (E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >XP_011660321.1 E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >KGN66894.1 hypothetical protein Csa_007170 [Cucumis sativus])

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 509/515 (98.83%), Postives = 510/515 (99.03%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60
           MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120
           VSIAEIGD ASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND
Sbjct: 61  VSIAEIGDSASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFH 180
           VRCIRAFT+SDLLYKQITN EEPEIPLLVALKERILEHASNFESFSLSLSS ADLQQPFH
Sbjct: 121 VRCIRAFTSSDLLYKQITNHEEPEIPLLVALKERILEHASNFESFSLSLSSGADLQQPFH 180

Query: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240
           LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360
           CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR
Sbjct: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360

Query: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420
           LAQNKRWRRCQECHRMIELTQGCFHMTC CGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE
Sbjct: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420

Query: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480
           NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP QSPPR
Sbjct: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPCQSPPR 480

Query: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515
           CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of Chy12G210900 vs. NCBI nr
Match: XP_008459101.1 (PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >XP_008459102.1 PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >KAA0043320.1 E3 ubiquitin-protein ligase arih1 [Cucumis melo var. makuwa] >TYK29385.1 E3 ubiquitin-protein ligase arih1 [Cucumis melo var. makuwa])

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 504/515 (97.86%), Postives = 511/515 (99.22%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60
           MEHR+IFGRGSSHGPAEEDDVE+FRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120
           VSIAEIGDPASGLSGIGV MEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND
Sbjct: 61  VSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFH 180
           VRCIRAFT+SDLLYKQITN+EEPEIPLLVALKERILEHASNFESFSL LSSSADL+QPFH
Sbjct: 121 VRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPFH 180

Query: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240
           LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQC+KTYVDGKVDASQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYE LENTLAEANIHADRIYCPFPNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360
           CLSARASSSSQS+NSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR
Sbjct: 301 CLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360

Query: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420
           LAQNKRWRRCQECHRMIELTQGCFHMTC CGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE
Sbjct: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420

Query: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480
           NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR
Sbjct: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480

Query: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515
           CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of Chy12G210900 vs. NCBI nr
Match: XP_038894440.1 (uncharacterized protein LOC120083025 [Benincasa hispida])

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 485/515 (94.17%), Postives = 502/515 (97.48%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60
           MEHR    RGSSHGPAEED+VEDFRSCCEDHEVWKETE+AVEEESE DLDEFSVKMFFKG
Sbjct: 1   MEHR----RGSSHGPAEEDEVEDFRSCCEDHEVWKETEEAVEEESE-DLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120
           VSIAEIGDPASGLSGIGV MEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND
Sbjct: 61  VSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFH 180
           V CIRAFT+SDLLYKQITN+E+PEIPLLVALKERILEHASNFE+FSL+LSSS DL+QP H
Sbjct: 121 VCCIRAFTDSDLLYKQITNEEDPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQPLH 180

Query: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQ 240
           LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCS CMKTYVDGKVDASQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSHCMKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIH+DRIYCPFPNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360
           CLSARASSSSQS+NSCIECPVCQRFICVECQVPWHSS+SCEEFQ++PLEERDTADITLHR
Sbjct: 301 CLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSMSCEEFQSVPLEERDTADITLHR 360

Query: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSE 420
           LAQNKRWRRCQECHRMIEL  GC+HMTC CGHEFCYSCGVEYQDG+QTCQCAFWDED+SE
Sbjct: 361 LAQNKRWRRCQECHRMIELAHGCYHMTCWCGHEFCYSCGVEYQDGEQTCQCAFWDEDDSE 420

Query: 421 NLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480
           N+VTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR
Sbjct: 421 NVVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPR 480

Query: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515
           CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 CTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 510

BLAST of Chy12G210900 vs. NCBI nr
Match: XP_022142466.1 (uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142467.1 uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142468.1 uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142469.1 uncharacterized protein LOC111012584 [Momordica charantia])

HSP 1 Score: 964 bits (2491), Expect = 0.0
Identity = 463/517 (89.56%), Postives = 489/517 (94.58%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESEED--LDEFSVKMFF 60
           MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKE ED VEE SEE+  LDEFSVKMFF
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGSEEEEHLDEFSVKMFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KG+SIAEIGDPASGLSGIGV MEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDVRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQP 180
           N++RCIRAFT+SDLLY QITN+EE EIPLL+ALKERILEHAS FE+FSL+LSSS DL+QP
Sbjct: 121 NEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDA 240
           FHLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMV LKCCHKFCS CMKTYVDGKV +
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGKVQS 240

Query: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYIT NEF+SFLPLTLYESLENTLAEANIH+DRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360
           SECLS RASSSSQS+NSCIECPVCQRFICV C VPWHSS+SCEEFQNI     DT DITL
Sbjct: 301 SECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTEDITL 360

Query: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDN 420
           HRLAQNKRW+RCQ+C RMIELTQGC+HMTC CGHEFCYSCG EYQD QQTCQCA WDE+N
Sbjct: 361 HRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALWDEEN 420

Query: 421 SENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP+QSP
Sbjct: 421 SEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPHQSP 480

Query: 481 PRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515
           PRCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 PRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

BLAST of Chy12G210900 vs. NCBI nr
Match: KAG7019427.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 949 bits (2452), Expect = 0.0
Identity = 458/518 (88.42%), Postives = 490/518 (94.59%), Query Frame = 0

Query: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEESE--EDLDEFSVKMFF 60
           M+HREIFGRGSS   AEEDD EDFRSCCED EVWK+ E A EE+SE  EDLDEFSVK+FF
Sbjct: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAEIGDPASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIA IGDPASG SGIGV MEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDVRCIRAFTNSDLLYKQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQP 180
           ND+ CIRAFT+SD LYKQITN+E+PEIPLLVALKERILEHASNFE+FSL+LSSS DL+QP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDA 240
           FHLAKVAIGVLS P+KGDKSVENCSICCDNKPSAM+VALKCCHKFCS C+KTYVDGKV+A
Sbjct: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYIT+NEF+SFLPLTLYESLENTLAEAN+ +DRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360
           SECLSARASSSSQS+NSC ECPVCQRFICVECQVPWHSS+SCEEFQN+PLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360

Query: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDN 420
             LAQNKRWRRCQEC RMIELTQGC+HMTC CGHEFCYSCG EYQDGQQTC+CA WDE+N
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420

Query: 421 SENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE++VTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDS-YVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515
           PRCT+S YVDTMKDIHQLPWLERFVS+ISDSYYEDYVQ
Sbjct: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

BLAST of Chy12G210900 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 649.0 bits (1673), Expect = 3.1e-186
Identity = 327/514 (63.62%), Postives = 403/514 (78.40%), Query Frame = 0

Query: 20  DVEDFRSCCEDHEVWKETED----AVEEESEEDL-DEFSVKMFFKGVSIAEIGDPASGLS 79
           D E+FRSCC D E+W +  D     VEEE ++DL DEFSVKM FKGVSI E GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQNDVRCIRAFTNSDLLY 139
           GIGV++E+SG +  +QVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A T+S+LLY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 KQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFHLAKVAIGVLSCPQK 199
            QIT +E+ EIPLLVAL+ER+LE  SN   F L L+   DL +   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQVPIRCPQLRCKYYI 259
           GDK +ENCSICC+++ S MM++LKC HKFCS CMKTYV+GKV  S+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 TSNEFKSFLPLTLYESLENTLAEANI---HADRIYCPFPNCSVLLDPSECLS---ARASS 319
           ++ E KSFLP+T ++S E    EAN+   +  +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSENS-CIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRW 379
           S+QSENS C+ECPVC+RF+CV+C VPWH+S+SCEEFQ +P++ER   DITLHRLA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSEN---LVT 439
           RRCQ+C  MIEL QGC HMTC CGHEFCYSCG EY++GQQTC CAFWD+D  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           +++QE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TD-SYVD-TMKDIHQLPWLERFVSVISDSYYEDY 514
           T+ SYV+  MKD+HQLPWLERFVSVISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

BLAST of Chy12G210900 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 649.0 bits (1673), Expect = 3.1e-186
Identity = 327/514 (63.62%), Postives = 403/514 (78.40%), Query Frame = 0

Query: 20  DVEDFRSCCEDHEVWKETED----AVEEESEEDL-DEFSVKMFFKGVSIAEIGDPASGLS 79
           D E+FRSCC D E+W +  D     VEEE ++DL DEFSVKM FKGVSI E GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQNDVRCIRAFTNSDLLY 139
           GIGV++E+SG +  +QVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A T+S+LLY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 KQITNQEEPEIPLLVALKERILEHASNFESFSLSLSSSADLQQPFHLAKVAIGVLSCPQK 199
            QIT +E+ EIPLLVAL+ER+LE  SN   F L L+   DL +   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGKVDASQVPIRCPQLRCKYYI 259
           GDK +ENCSICC+++ S MM++LKC HKFCS CMKTYV+GKV  S+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 TSNEFKSFLPLTLYESLENTLAEANI---HADRIYCPFPNCSVLLDPSECLS---ARASS 319
           ++ E KSFLP+T ++S E    EAN+   +  +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSENS-CIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHRLAQNKRW 379
           S+QSENS C+ECPVC+RF+CV+C VPWH+S+SCEEFQ +P++ER   DITLHRLA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQECHRMIELTQGCFHMTCCCGHEFCYSCGVEYQDGQQTCQCAFWDEDNSEN---LVT 439
           RRCQ+C  MIEL QGC HMTC CGHEFCYSCG EY++GQQTC CAFWD+D  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           +++QE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TD-SYVD-TMKDIHQLPWLERFVSVISDSYYEDY 514
           T+ SYV+  MKD+HQLPWLERFVSVISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

BLAST of Chy12G210900 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 183.0 bits (463), Expect = 6.3e-46
Identity = 118/379 (31.13%), Postives = 202/379 (53.30%), Query Frame = 0

Query: 55  KMFFKGVSIAE--IGDPASGLSGIGV-LMEKSGKIPFVQVQKKLDFYVDELVADYLALMD 114
           +++FKG+   E   G     +SG GV + ++   + F      +D  +    A+  AL+ 
Sbjct: 146 RLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPLIDNGMSRQGAELKALIR 205

Query: 115 GLVEAMQNDVRCIRAFTNSDLLYKQITNQ---EEPEIPLLVALKERILEHASNFESFSLS 174
           GL EA++  ++ I  F +S  +++ +T +   ++ +I LL+   + I++H   F S+   
Sbjct: 206 GLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQH---FSSYQHV 265

Query: 175 LSSSADLQQPFHLAKVAIGVLSCPQKGDKSV-----ENCSICCDNKPSAMMVAL-KCCHK 234
           L +  D++  + LA+ +I  +  P +  +       E C+IC ++  +  M ++ KC H+
Sbjct: 266 LVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHR 325

Query: 235 FCSQCMKTYVDGKVDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIH- 294
           FC QC+K +V+ K+     P +CP   CK  +  +     L   L +  +  L E  I  
Sbjct: 326 FCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPV 385

Query: 295 ADRIYCPFPNCSVLLDPSECL-SARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSC 354
            +R+YCP+P CS L+  ++   SA++  S   ++    C  C+   CV+C+VPWH ++SC
Sbjct: 386 TERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSC 445

Query: 355 EEFQNIPLEERDTADITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSCGV 414
            E++ +   E    D+ L  LA NK WR+C +C  MIEL+QGC H+TC CGHEFCY+CG 
Sbjct: 446 TEYKKLH-PEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGG 505

Query: 415 EYQDGQQTC--QCAFWDED 418
            +     TC  +C  W+E+
Sbjct: 506 GWNKIMGTCLNRCPTWNEE 519

BLAST of Chy12G210900 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.9e-42
Identity = 107/311 (34.41%), Postives = 172/311 (55.31%), Query Frame = 0

Query: 107 LALMDGLVEAMQNDVRCIRAFTNSDLLYKQITNQE--EPEIPLLVALKERILEHASNFES 166
           +AL+ GL E+    +R +  + +  LLY+ I  +   + ++  LV   + +LE  +  ++
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKKKVVHLVDEFQLLLEEMTYTDA 60

Query: 167 FSLSLSSSADLQQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMM--VALKCCHK 226
             ++L+   D+   F LA+ AI       + D   E CSIC +      M     KC H+
Sbjct: 61  DLIALN---DVNFAFKLAREAI-----VSRDDVKAEICSICFEETEGERMFFTTEKCVHR 120

Query: 227 FCSQCMKTYVDGKVDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANI-H 286
            C  C+K YV+ K+ +  VP  C    CK+ +T       L L L E  +  + E +I  
Sbjct: 121 HCFPCVKQYVEVKLLSGTVP-TCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPA 180

Query: 287 ADRIYCPFPNCSVLLDPSECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCE 346
           A+RIYCP+PNCS+L+  +E  S    S   ++ SC++C  C  F C++C+VP HS +SC 
Sbjct: 181 AERIYCPYPNCSMLMSKTELSS---ESDLSNDRSCVKC--CGLF-CIDCKVPSHSDLSCA 240

Query: 347 EFQNI---PLEERDTADITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCYSC 406
           E++ +   PL +    ++ L  LA++K+WR+C+ C  MIEL+  C HMTC CG++FCY C
Sbjct: 241 EYKKLHHDPLVD----ELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQC 292

Query: 407 GVEYQDGQQTC 410
            VE+++ Q+TC
Sbjct: 301 EVEWKNDQKTC 292

BLAST of Chy12G210900 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 164.1 bits (414), Expect = 3.0e-40
Identity = 115/367 (31.34%), Postives = 191/367 (52.04%), Query Frame = 0

Query: 49  LDEFSVKMFFKG-VSIAEIGDPASGL----SGIGVLMEKSGKIPFVQVQKKLDFYVDELV 108
           +D    ++ FKG V+   + D          G+ +  EK   +  ++ +   D  +    
Sbjct: 149 MDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIK-ESLRDIEISRRG 208

Query: 109 ADYLALMDGLVEAMQNDVRCIRAFTNSDLLYKQI--TNQEEPEIPLLVALKERILEHASN 168
            + +AL+ GL E+    +R +  + + D +Y+ I    + + +I  LV   + ILE  + 
Sbjct: 209 VEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKKKIDHLVEEVQGILEKMAC 268

Query: 169 FESFSLSLSSSADLQQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKP-SAMMVALKCC 228
            ++    L +  D++  F LA+ AIG  S     ++  E C I  +      M V  KC 
Sbjct: 269 IDAV---LVARNDVKFAFRLAREAIGRNSVDVNAEQG-ETCGIFFEETDVEHMFVTEKCL 328

Query: 229 HKFCSQCMKTYVDGKVDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANI 288
           H+ C  C+K +V  K+ +   P  C +  CK+ +T       L L L E  +  + E +I
Sbjct: 329 HRHCFPCVKQHVKVKLRSGTEP-TCLEYGCKFKLTLERCSKVLTLKLIEMWKQKMKEDSI 388

Query: 289 -HADRIYCPFPNCSVLLDPSECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVS 348
             A+RIYCP+PNCS+L+  +E LS+ A  S  +  +C++C  C  F C++C+VP H+ +S
Sbjct: 389 PAAERIYCPYPNCSMLMSKTE-LSSEADLS--NVRTCVKC--CGLF-CIDCKVPSHTDLS 448

Query: 349 CEEFQNI---PLEERDTADITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCCCGHEFCY 404
            ++++ +   PL +    D+ L  LA +K WR+C +C  MIEL+ GC HMTC CG+EFCY
Sbjct: 449 YDDYKKLHPDPLVD----DLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYEFCY 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
D3YYI72.2e-1930.88Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P... [more]
Q8TC412.9e-1930.88Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE... [more]
Q4KLT02.7e-1728.64Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 ... [more]
Q6T4865.6e-1526.57Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
A2VEA38.9e-1326.96E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M0W54.1e-30398.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G706610 PE=3 SV=1[more]
A0A1S3C9E44.5e-30297.86E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 S... [more]
A0A5D3E1X14.5e-30297.86E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1CLL91.2e-27889.56uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A6J1EGQ63.4e-27387.84ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita mo... [more]
Match NameE-valueIdentityDescription
XP_004145453.10.098.83E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >XP_011660321.1 E3 ubiquitin... [more]
XP_008459101.10.097.86PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >XP_008459102.1 PRED... [more]
XP_038894440.10.094.17uncharacterized protein LOC120083025 [Benincasa hispida][more]
XP_022142466.10.089.56uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142467.1 uncha... [more]
KAG7019427.10.088.42putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. arg... [more]
Match NameE-valueIdentityDescription
AT4G19670.13.1e-18663.62RING/U-box superfamily protein [more]
AT4G19670.23.1e-18663.62RING/U-box superfamily protein [more]
AT5G60250.16.3e-4631.13zinc finger (C3HC4-type RING finger) family protein [more]
AT2G25360.11.9e-4234.41RING/U-box superfamily protein [more]
AT2G25370.13.0e-4031.34RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 350..409
e-value: 0.11
score: 12.7
coord: 265..340
e-value: 8.4E-9
score: 45.2
IPR002867IBR domainPFAMPF01485IBRcoord: 279..340
e-value: 1.4E-8
score: 34.9
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 202..248
e-value: 0.0018
score: 27.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 202..249
score: 10.704165
NoneNo IPR availableGENE3D1.20.120.1750coord: 337..464
e-value: 1.9E-14
score: 56.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR11685:SF353RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 18..514
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 197..276
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 353..417
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 54..187
e-value: 3.6E-5
score: 25.7
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 71..169
e-value: 6.8E-8
score: 32.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 196..282
e-value: 1.3E-14
score: 55.9
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 18..514
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 219..228
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 198..415
score: 28.074995

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G210900.1Chy12G210900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0004386 helicase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity