Chy12G210650 (gene) Cucumber (hystrix) v1

Overview
NameChy12G210650
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionalpha-glucosidase
LocationchrH12: 5875626 .. 5880434 (-)
RNA-Seq ExpressionChy12G210650
SyntenyChy12G210650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGTGGTTCGTCCAAAGTCATAAGAACGCCATTTCCTCACTCTCTCCTAATACTAATACTCTTTCTTTTCACTTCCTTCCTCCCTCTACCCGCTACTTCTCTACCAGCCGTCGGATTTGGATACCGAATCAGATCCAGCCACGTCGATCCTGCCGGAAAGTCATTGACCGCCGATCTTGACCTCATCGGTACCTCCCAAGTCTATGGACCTGATCTTCCCACACTTACCCTCCAAGCCACGTAAATTTCTTTCTTCTTCTCTTCTCTTCTTTAATCTATTTTATTTTATTTTGGGTTTATTTCAATTTTCAGAAAACCAAAATATTTATAAATATGGGGCAATATCTATTAGTTAAATACTACTTATATTTCTATATATTTTAAGCATTTATCATACTTTCATTTATCTGAAATTGGTTTTTTTTTGTTGGAGAGAGAACGAAATTAGAGAAATGATTTTGCTCGGTGTTCTTAGAAATTGGAATGAGTTGGGAATAAATGCCTTCTAAACCAAATCATATTTTCTAAAACATCTTTATCAAAAAGTTAATATTTAAATTGCGCAAATTTTGTATGGCTGTTAGAATGCTTTTTTTAATAGAATAGTTGCATGTTAAAAAATTTATAAATATAGCAAATTTATTAATAAAAGAAATCTATTACTAATAGACTACCTTATAAATATTTGTATATTAATGATTGAGCGTATTTATAATTTTTAAAAAATATTATCATATACTTGAGTATTACTTCAATTATAATTATCTTTTTCTAATTTTAATAAATAAAAAATCTTTTCAAATATTATTATTAATGAAAAAAATTATGAAAGTTGATTACAATCATTTATTTTTTTAGAACTATAATTATTACATTTCACTATTTTTTAGACCCTTCAAATGAATACTAAAATTTCACTTCATAATAATTTTTAGTTAATTAATAAGCAATTAACTTGAAACACAAGGAAAATTTTAGGTTAAAACTAAAAATATAAATCTTAAAACCGCTAGATCAAAAGTTAAAATAAAAAAGTCAAATTTGATCGGAAAAAATTAAAAATAATTTGATTTCAAATTGATTTTGAAATTAATTGAAATCACATTTAAAATTTTTTAAAAAAATTATTCCACCAAATGAACAACCAAAACTGAGATAAAAACGTATTATTTTTCCTATAAATGAAAAGTTGTTTTAGGCAACACTAAAAGAGCTAACACGTCGTTACAGGTGAGGAAAAACACGGTTAAAGAAAGAACATCTTTTTACATCAGAAAATCAACGTGGGAGGAAGCCCAAGGAACTTTGTTAGTTTGTTTCTTCCCCTTGAGAAAAAAGAGAAGCAGACTAACGGAGTTTCCAACATACAGATTATCTTTTGTTTTTTAAAATAATAATATGATCTATGGTATGCAGTTTTGAAAGCAAAGATCGGTTGAGAGTACGAATAACGGACTCAACTCGTGAACGGTGGGAGATTCCACACCATATTATTCCCCGTCGATCGAACTCCCTTATCCGTTCACTGCCGGAAAACCATGTCGCCTCCCCGAAAGCTTCTTTCATCTCCGATCCAGCTTCCGATCTCATTTTCACCCTCCACGACACTGCACCCTTCGGCTTTTCCGTGCTCCGACGATCATCCGGTGATGTCCTCTTTGACACATCCCCTGATTTCTCCGATTCCGAGACTTTTCTTGTCTTCAAGGATCAATACATTCAACTATCCTCTTCGCTTCCCAAGGACAGATCCTCTATCTTTGGCATTGGCGAGCAAACCCGGAAGTCGTTCAAGCTCGTCCCGGATAAAAACAAAACCCTAACTCTTTGGAACGCCGATATTGGTAGTGTGAATCTCGATGTGAACCTCTATGGTGCACATCCTTTCTACATTGATCTCCGGTCGCCGTCTCAAGATGGTAAAGTTGCGGCAGGGACGACGCATGGAGTTTTGTTGCTCAACAGTAATGGCATGGATATTATATACTCTGGCGATAGGATTACGTACAAGGTTATTGGTGGAATCATTGATTTATACTTCTTTGCGGGTCCGTCACCAATTTCGGTTATAGATCAGTATACTGAGCTTATTGGGCGGCCTGCACCTGTGCCTTATTGGTCGTTTGGTTAGTATTTCTATTGTCTTGATGTTTATGTCTGTTGGATTCTTTTGAGATTTCATGTACATTGGCAGTTAGTTTTGAATTTCATGATTTTTTTGAATTTGTGCATCAAATCGGAAAGTTCAATTTATGTAGAACAGAAGATTTCATGGATTCGTGTTTTGTCCTCTCGTTATTTCCTCAGGGTTTTGCAACATCGGGTTACGAGTTGATCCTGTGGGTTTTAGACAGACAAAATTTGAATAGAAACTGATATTTCTTTATTTGACTTTCTGTTCTTTAATCGAGTTTTTCCCATTTGAAATAGATAAGAAAGATAGTTTTCAGTATTGCTACTTAAAGTGAATTCATACCCGGGTGAATTACTTCTAGCTAATTGCTTGTCAAAATCACTATTTTTTTTTTTTTGAAATTCAACAGCATGTGGGAGTAAGGAGATCCAAATTTGACCTCATGGTCGAGGGATATGCCTTAACTAGTTGAATTCTGCTCATGTTTGCTTATGTTTATACTCGAGTCACTTTATTAGGAAAACACATGCATTCTTTTAGACTCTAATTTATCTTTCAGTTTGAGCTATTCATTTCTGTTTTCTAATCATTCTATGCCACAAATAATAAATAGTAAACTAAATGTTCTTGTGTGAAGGTTTTCATCAATGTCGTTACGGCTACAACAATGTTTCTGACATTGAAAGTGTAGTTGCTCGTTATGCCAAAGCCAGTATACCTCTTGAAGCTATGTGGACAGACATTGATTACATGGATGGGTATAAAGACTTTACTTTTGATCCCATCAATTTTCCATCGGACAAGATGAAGATATTTGTTGATAATCTTCACAAAAATGGACAAAAATATGTACTCATCTTGGATCCTGGTATGTTATTTAGCAATGCAATTCTTTTTTCATGTATGGAAAATGATGGAAATATTTCTTCTCTTCCTTTCTTCCTTTTTTTTTCAGCCCACACGCAATGCAAGCTCATGTCTTTACTGTTAATTATATGCAGGTATTAGTACAAATAATACATATGGACCATACATTCGAGGAACAGAAGCTGATATCTTTATGAAATACAACGGGGTTCCATACTTGGGTGATGTTTGGCCTGGACCTGTCTACTTTCCTGATTTTTTTCATCCAGACAGTGAGACTTTTTGGGGTCGTGAGATCCAAATATTTCGAGATATTGTTCCATTTGACGGTCTTTGGATCGATATGAATGAGATATCAAATTTTATAACTTCTTCAACCAACCCACTTTCTAACCTTGATAACCCTCCTTACATGATTAACAATGCTAGAGTTCAACGTCCCCTTAATAATAAGACTGTGCCAGCGTCAATTCTTCATTTTGGTAACATGGCAGAGTATAATACTCATAACCTATACGGGTTCTTAGAGTCAAGGGCTACTCATGCCTCATTAGTAAAAGTAACAGGCCAGAGACCATTTGTGCTGTCAAGATCAACTTTTGTAGGCTCTGGGAAGTACACAGCACATTGGACCGGAGATATTGGTGCGACGTGGAATGATTTGGGGTACACAATTCCATCCATTTTGAACTTTGGACTCTTCGGAATTCCTATGGTTGGTTCTGACATATGTGGATTTTCCGGAGATACGACAGAAGAGCTTTGCCGACGTTGGATTCAGGTGCAAGTTTATCTTGCCATTTTATTATTCTAGATGATGTGTTGGAAGACACTGTTTACCAAAACACTAAAGTTTTGCTATTTGGTAGGAAAGATTACAACTAACTGAAATAAAAACTTGTTCAACTTTTGCAGCTAGGTGCATTTTACCCATTTGCCAGAGATCATTCTGATAAAGGATCCATTCGTCAAGAGCTTTATCTTTGGGATTCAGTTGCTGCATCAGCCAGGAAGGTGCTTGCGCTTCGCTATCAGTTACTTCCTTATTTCTACACTTTGATGTATGAGGCACACAAAAAGGGGACACCGATCGCACGTCCTCTCTTCTTCTCATTCCCTCAGGATATCAAGACCTACGAAATCGACTCTCAGTTCCTACTTGGAGGAGGTGTATTGGTTTCTCCAGTTCTAAAGGAGGGAGCTTTTTCTGTTGAGGCATATTTCCCTGCAGGAAACTGGTTTAGCCTCTTTAACTATTCAGAGTTCGTAGCTGTAAATTCCGGGCAACAAATCAATCTTGATGCACCTGCTGATCACATAAACGTGCACGTCAGGGAAGGAAACATTCTGGCATTACACGGAGAGGCGATGACAACGCGAGCAGCCCAAGAGACCCCATACAAGCTTTTGGTCGTCATCAGCAATGGTCAAAGCAGCTTTGGAGAGGTTTTCTTGGATGATGGAGAAGTGGTGGAAATGGGAAGAGAAGGAGGTAATTGGAGTATGGTAAGATTTTATAGTGAAACAGTTGGGAGTAAGTTAGTAGTGAAATCTCAGGTTATAAATGGAGGATTTGCCTTGAGCCAAAAACTGATTATTGACAAGGTAACCTTTGTAGGCTTTGAAAGGCCTAAGAAAATGGTTGACCTAGGTTTAAATATAAGCAAGGGATTGAACTTGAATGGAAATTCAAGCATTAGAAAAACCTATCAATATTTTGCCAAGTTTATGAATGCTGAGATCTCAGGGCTATCCATTCCTATTTGGGAGGAATTCATATTGGAGATGACACCAATAAATTGA

mRNA sequence

ATGGCGAGTGGTTCGTCCAAAGTCATAAGAACGCCATTTCCTCACTCTCTCCTAATACTAATACTCTTTCTTTTCACTTCCTTCCTCCCTCTACCCGCTACTTCTCTACCAGCCGTCGGATTTGGATACCGAATCAGATCCAGCCACGTCGATCCTGCCGGAAAGTCATTGACCGCCGATCTTGACCTCATCGGTACCTCCCAAGTCTATGGACCTGATCTTCCCACACTTACCCTCCAAGCCACTTTTGAAAGCAAAGATCGGTTGAGAGTACGAATAACGGACTCAACTCGTGAACGGTGGGAGATTCCACACCATATTATTCCCCGTCGATCGAACTCCCTTATCCGTTCACTGCCGGAAAACCATGTCGCCTCCCCGAAAGCTTCTTTCATCTCCGATCCAGCTTCCGATCTCATTTTCACCCTCCACGACACTGCACCCTTCGGCTTTTCCGTGCTCCGACGATCATCCGGTGATGTCCTCTTTGACACATCCCCTGATTTCTCCGATTCCGAGACTTTTCTTGTCTTCAAGGATCAATACATTCAACTATCCTCTTCGCTTCCCAAGGACAGATCCTCTATCTTTGGCATTGGCGAGCAAACCCGGAAGTCGTTCAAGCTCGTCCCGGATAAAAACAAAACCCTAACTCTTTGGAACGCCGATATTGGTAGTGTGAATCTCGATGTGAACCTCTATGGTGCACATCCTTTCTACATTGATCTCCGGTCGCCGTCTCAAGATGGTAAAGTTGCGGCAGGGACGACGCATGGAGTTTTGTTGCTCAACAGTAATGGCATGGATATTATATACTCTGGCGATAGGATTACGTACAAGGTTATTGGTGGAATCATTGATTTATACTTCTTTGCGGGTCCGTCACCAATTTCGGTTATAGATCAGTATACTGAGCTTATTGGGCGGCCTGCACCTGTGCCTTATTGGTCGTTTGGTTTTCATCAATGTCGTTACGGCTACAACAATGTTTCTGACATTGAAAGTGTAGTTGCTCGTTATGCCAAAGCCAGTATACCTCTTGAAGCTATGTGGACAGACATTGATTACATGGATGGGTATAAAGACTTTACTTTTGATCCCATCAATTTTCCATCGGACAAGATGAAGATATTTGTTGATAATCTTCACAAAAATGGACAAAAATATGTACTCATCTTGGATCCTGGTATTAGTACAAATAATACATATGGACCATACATTCGAGGAACAGAAGCTGATATCTTTATGAAATACAACGGGGTTCCATACTTGGGTGATGTTTGGCCTGGACCTGTCTACTTTCCTGATTTTTTTCATCCAGACAGTGAGACTTTTTGGGGTCGTGAGATCCAAATATTTCGAGATATTGTTCCATTTGACGGTCTTTGGATCGATATGAATGAGATATCAAATTTTATAACTTCTTCAACCAACCCACTTTCTAACCTTGATAACCCTCCTTACATGATTAACAATGCTAGAGTTCAACGTCCCCTTAATAATAAGACTGTGCCAGCGTCAATTCTTCATTTTGGTAACATGGCAGAGTATAATACTCATAACCTATACGGGTTCTTAGAGTCAAGGGCTACTCATGCCTCATTAGTAAAAGTAACAGGCCAGAGACCATTTGTGCTGTCAAGATCAACTTTTGTAGGCTCTGGGAAGTACACAGCACATTGGACCGGAGATATTGGTGCGACGTGGAATGATTTGGGGTACACAATTCCATCCATTTTGAACTTTGGACTCTTCGGAATTCCTATGGTTGGTTCTGACATATGTGGATTTTCCGGAGATACGACAGAAGAGCTTTGCCGACGTTGGATTCAGCTAGGTGCATTTTACCCATTTGCCAGAGATCATTCTGATAAAGGATCCATTCGTCAAGAGCTTTATCTTTGGGATTCAGTTGCTGCATCAGCCAGGAAGGTGCTTGCGCTTCGCTATCAGTTACTTCCTTATTTCTACACTTTGATGTATGAGGCACACAAAAAGGGGACACCGATCGCACGTCCTCTCTTCTTCTCATTCCCTCAGGATATCAAGACCTACGAAATCGACTCTCAGTTCCTACTTGGAGGAGGTGTATTGGTTTCTCCAGTTCTAAAGGAGGGAGCTTTTTCTGTTGAGGCATATTTCCCTGCAGGAAACTGGTTTAGCCTCTTTAACTATTCAGAGTTCGTAGCTGTAAATTCCGGGCAACAAATCAATCTTGATGCACCTGCTGATCACATAAACGTGCACGTCAGGGAAGGAAACATTCTGGCATTACACGGAGAGGCGATGACAACGCGAGCAGCCCAAGAGACCCCATACAAGCTTTTGGTCGTCATCAGCAATGGTCAAAGCAGCTTTGGAGAGGTTTTCTTGGATGATGGAGAAGTGGTGGAAATGGGAAGAGAAGGAGGTAATTGGAGTATGGTAAGATTTTATAGTGAAACAGTTGGGAGTAAGTTAGTAGTGAAATCTCAGGTTATAAATGGAGGATTTGCCTTGAGCCAAAAACTGATTATTGACAAGGTAACCTTTGTAGGCTTTGAAAGGCCTAAGAAAATGGTTGACCTAGGTTTAAATATAAGCAAGGGATTGAACTTGAATGGAAATTCAAGCATTAGAAAAACCTATCAATATTTTGCCAAGTTTATGAATGCTGAGATCTCAGGGCTATCCATTCCTATTTGGGAGGAATTCATATTGGAGATGACACCAATAAATTGA

Coding sequence (CDS)

ATGGCGAGTGGTTCGTCCAAAGTCATAAGAACGCCATTTCCTCACTCTCTCCTAATACTAATACTCTTTCTTTTCACTTCCTTCCTCCCTCTACCCGCTACTTCTCTACCAGCCGTCGGATTTGGATACCGAATCAGATCCAGCCACGTCGATCCTGCCGGAAAGTCATTGACCGCCGATCTTGACCTCATCGGTACCTCCCAAGTCTATGGACCTGATCTTCCCACACTTACCCTCCAAGCCACTTTTGAAAGCAAAGATCGGTTGAGAGTACGAATAACGGACTCAACTCGTGAACGGTGGGAGATTCCACACCATATTATTCCCCGTCGATCGAACTCCCTTATCCGTTCACTGCCGGAAAACCATGTCGCCTCCCCGAAAGCTTCTTTCATCTCCGATCCAGCTTCCGATCTCATTTTCACCCTCCACGACACTGCACCCTTCGGCTTTTCCGTGCTCCGACGATCATCCGGTGATGTCCTCTTTGACACATCCCCTGATTTCTCCGATTCCGAGACTTTTCTTGTCTTCAAGGATCAATACATTCAACTATCCTCTTCGCTTCCCAAGGACAGATCCTCTATCTTTGGCATTGGCGAGCAAACCCGGAAGTCGTTCAAGCTCGTCCCGGATAAAAACAAAACCCTAACTCTTTGGAACGCCGATATTGGTAGTGTGAATCTCGATGTGAACCTCTATGGTGCACATCCTTTCTACATTGATCTCCGGTCGCCGTCTCAAGATGGTAAAGTTGCGGCAGGGACGACGCATGGAGTTTTGTTGCTCAACAGTAATGGCATGGATATTATATACTCTGGCGATAGGATTACGTACAAGGTTATTGGTGGAATCATTGATTTATACTTCTTTGCGGGTCCGTCACCAATTTCGGTTATAGATCAGTATACTGAGCTTATTGGGCGGCCTGCACCTGTGCCTTATTGGTCGTTTGGTTTTCATCAATGTCGTTACGGCTACAACAATGTTTCTGACATTGAAAGTGTAGTTGCTCGTTATGCCAAAGCCAGTATACCTCTTGAAGCTATGTGGACAGACATTGATTACATGGATGGGTATAAAGACTTTACTTTTGATCCCATCAATTTTCCATCGGACAAGATGAAGATATTTGTTGATAATCTTCACAAAAATGGACAAAAATATGTACTCATCTTGGATCCTGGTATTAGTACAAATAATACATATGGACCATACATTCGAGGAACAGAAGCTGATATCTTTATGAAATACAACGGGGTTCCATACTTGGGTGATGTTTGGCCTGGACCTGTCTACTTTCCTGATTTTTTTCATCCAGACAGTGAGACTTTTTGGGGTCGTGAGATCCAAATATTTCGAGATATTGTTCCATTTGACGGTCTTTGGATCGATATGAATGAGATATCAAATTTTATAACTTCTTCAACCAACCCACTTTCTAACCTTGATAACCCTCCTTACATGATTAACAATGCTAGAGTTCAACGTCCCCTTAATAATAAGACTGTGCCAGCGTCAATTCTTCATTTTGGTAACATGGCAGAGTATAATACTCATAACCTATACGGGTTCTTAGAGTCAAGGGCTACTCATGCCTCATTAGTAAAAGTAACAGGCCAGAGACCATTTGTGCTGTCAAGATCAACTTTTGTAGGCTCTGGGAAGTACACAGCACATTGGACCGGAGATATTGGTGCGACGTGGAATGATTTGGGGTACACAATTCCATCCATTTTGAACTTTGGACTCTTCGGAATTCCTATGGTTGGTTCTGACATATGTGGATTTTCCGGAGATACGACAGAAGAGCTTTGCCGACGTTGGATTCAGCTAGGTGCATTTTACCCATTTGCCAGAGATCATTCTGATAAAGGATCCATTCGTCAAGAGCTTTATCTTTGGGATTCAGTTGCTGCATCAGCCAGGAAGGTGCTTGCGCTTCGCTATCAGTTACTTCCTTATTTCTACACTTTGATGTATGAGGCACACAAAAAGGGGACACCGATCGCACGTCCTCTCTTCTTCTCATTCCCTCAGGATATCAAGACCTACGAAATCGACTCTCAGTTCCTACTTGGAGGAGGTGTATTGGTTTCTCCAGTTCTAAAGGAGGGAGCTTTTTCTGTTGAGGCATATTTCCCTGCAGGAAACTGGTTTAGCCTCTTTAACTATTCAGAGTTCGTAGCTGTAAATTCCGGGCAACAAATCAATCTTGATGCACCTGCTGATCACATAAACGTGCACGTCAGGGAAGGAAACATTCTGGCATTACACGGAGAGGCGATGACAACGCGAGCAGCCCAAGAGACCCCATACAAGCTTTTGGTCGTCATCAGCAATGGTCAAAGCAGCTTTGGAGAGGTTTTCTTGGATGATGGAGAAGTGGTGGAAATGGGAAGAGAAGGAGGTAATTGGAGTATGGTAAGATTTTATAGTGAAACAGTTGGGAGTAAGTTAGTAGTGAAATCTCAGGTTATAAATGGAGGATTTGCCTTGAGCCAAAAACTGATTATTGACAAGGTAACCTTTGTAGGCTTTGAAAGGCCTAAGAAAATGGTTGACCTAGGTTTAAATATAAGCAAGGGATTGAACTTGAATGGAAATTCAAGCATTAGAAAAACCTATCAATATTTTGCCAAGTTTATGAATGCTGAGATCTCAGGGCTATCCATTCCTATTTGGGAGGAATTCATATTGGAGATGACACCAATAAATTGA

Protein sequence

MASGSSKVIRTPFPHSLLILILFLFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLPENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFILEMTPIN*
Homology
BLAST of Chy12G210650 vs. ExPASy Swiss-Prot
Match: O04893 (Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 531/899 (59.07%), Postives = 670/899 (74.53%), Query Frame = 0

Query: 16  SLLILILFLFTSFLPLPATS----LPAVGFGYRIRSSHVDP-AGKSLTADLDLIGTSQVY 75
           +L IL++FL    +   +TS       +G+GY+++S  VD    +SLTA   L+  S VY
Sbjct: 9   ALGILLVFLLQYLVAGISTSENDPEGVIGYGYKVKSVKVDSGTRRSLTALPQLVKNSSVY 68

Query: 76  GPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHII---------PRRSNSLIRSLPE 135
           GPD+  L++ A+ ES DRLRVRITD+   RWEIP +I+         P   +SL R+L  
Sbjct: 69  GPDIQLLSITASLESNDRLRVRITDAKHRRWEIPDNILHRHQPPPPPPHSLSSLYRTLLS 128

Query: 136 NHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQ 195
           +   + +   +S P SDL F+L +T PFGF++ R+S+ DVLFD +PD ++  TFL+F DQ
Sbjct: 129 SPTTNRRKILLSHPNSDLTFSLINTTPFGFTISRKSTHDVLFDATPDPTNPNTFLIFIDQ 188

Query: 196 YIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYI 255
           Y+ L+SSLP  R+ I+G+GE ++ +F+L    N+TLT+  ADI S N DVNLYG+HPFY+
Sbjct: 189 YLHLTSSLPGTRAHIYGLGEHSKPTFQLA--HNQTLTMRAADIPSSNPDVNLYGSHPFYM 248

Query: 256 DLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVID 315
           D+RS        AG+THGVLLLNSNGMD+ Y+G+RITYKVIGGIIDLYFFAGPSP  V++
Sbjct: 249 DVRS-----SPVAGSTHGVLLLNSNGMDVEYTGNRITYKVIGGIIDLYFFAGPSPGQVVE 308

Query: 316 QYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYK 375
           Q+T +IGRPAP+PYW+FGF QCRYGY++V +++SVVA YAKA IPLE MWTDIDYMD YK
Sbjct: 309 QFTRVIGRPAPMPYWAFGFQQCRYGYHDVYELQSVVAGYAKAKIPLEVMWTDIDYMDAYK 368

Query: 376 DFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGV 435
           DFT DP+NFP DKMK FV+NLHKNGQKYV+ILDPGISTN TY  YIRG + D+F+K NG 
Sbjct: 369 DFTLDPVNFPLDKMKKFVNNLHKNGQKYVVILDPGISTNKTYETYIRGMKHDVFLKRNGK 428

Query: 436 PYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLS 495
           PYLG VWPGPVYFPDF  P + TFW  EI+ F +++P DGLWIDMNEISNFI+S   P S
Sbjct: 429 PYLGSVWPGPVYFPDFLKPSALTFWTDEIKRFLNLLPVDGLWIDMNEISNFISSPPIPGS 488

Query: 496 NLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQ 555
            LDNPPY INN+ V  P+ NKT+P + +H+G++ EYN HNL+G+LE+R T A+L+K+T +
Sbjct: 489 TLDNPPYKINNSGVMLPIINKTIPPTAMHYGDIPEYNVHNLFGYLEARVTRAALIKLTEK 548

Query: 556 RPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDT 615
           RPFVLSRSTF GSGKYTAHWTGD  ATWNDL Y+IPS+L+FGLFGIPMVG+DICGF G+T
Sbjct: 549 RPFVLSRSTFSGSGKYTAHWTGDNAATWNDLVYSIPSMLDFGLFGIPMVGADICGFLGNT 608

Query: 616 TEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMY 675
           TEELCRRWIQLGAFYPF+RDHS  G+  QELY W+SVAASARKVL LRY LLPYFYTLMY
Sbjct: 609 TEELCRRWIQLGAFYPFSRDHSSLGTTYQELYRWESVAASARKVLGLRYTLLPYFYTLMY 668

Query: 676 EAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFS 735
           EA   G PIARPLFFSFP DIKTY I SQFLLG GV+VSPVLK G  SV AYFP GNWF 
Sbjct: 669 EAQLNGIPIARPLFFSFPDDIKTYGISSQFLLGKGVMVSPVLKPGVVSVTAYFPRGNWFD 728

Query: 736 LFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISN 795
           LF+Y+  V  ++G+ + L AP DHINVH++EGNILA+ G+AMTT+AA++TP+ LLVV+S+
Sbjct: 729 LFDYTRSVTASTGRYVTLSAPPDHINVHIQEGNILAMQGKAMTTQAARKTPFHLLVVMSD 788

Query: 796 GQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDK 855
             +SFGE+FLDDG  V MG   G W+ V+F + +     ++ S V++G FA+SQK +IDK
Sbjct: 789 CGASFGELFLDDGVEVTMGVNRGKWTFVKFIAASAKQTCIITSDVVSGEFAVSQKWVIDK 848

Query: 856 VTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFIL 901
           VT +G  +  K+    +           S ++ T     +F+ AEISGL++ +  EF L
Sbjct: 849 VTILGLRKGTKINGYTVRTGAVTRKGDKSKLKSTPDRKGEFIVAEISGLNLLLGREFKL 900

BLAST of Chy12G210650 vs. ExPASy Swiss-Prot
Match: O04931 (Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1)

HSP 1 Score: 1045.0 bits (2701), Expect = 4.8e-304
Identity = 495/877 (56.44%), Postives = 657/877 (74.91%), Query Frame = 0

Query: 38  AVGFGYRIRSSHVD-PAGKSLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDS 97
           A+G+GY+++++ VD   GKSLTA L LI  S VYGPD+  L+  A+FE  D LR+R TD+
Sbjct: 40  AIGYGYQVKNAKVDNSTGKSLTALLQLIRNSPVYGPDIHFLSFTASFEEDDTLRIRFTDA 99

Query: 98  TRERWEIPHHIIPR-----------RSNSLIRSLPENHVASPKASFISDPASDLIFTLHD 157
              RWEIP+ ++PR               L + +P+N    P  + +S P SDL FTL  
Sbjct: 100 NNRRWEIPNEVLPRPPPPPSPPPLSSLQHLPKPIPQN---QPTTTVLSHPHSDLAFTLFH 159

Query: 158 TAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRK 217
           T PFGF++ R+S+ DVLFD +P  S+  TFL++KDQY+QLSSSLP  ++ ++G+GE T+ 
Sbjct: 160 TTPFGFTIYRKSTHDVLFDATPIPSNPTTFLIYKDQYLQLSSSLPAQQAHLYGLGEHTKP 219

Query: 218 SFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNS 277
           +F+L    N+ LTLWNADI S N D+NLYG+HPFY+D+RS         G+THGV LLNS
Sbjct: 220 TFQLA--HNQILTLWNADIASFNRDLNLYGSHPFYMDVRSSPM-----VGSTHGVFLLNS 279

Query: 278 NGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRY 337
           NGMD+ Y+GDRITYKVIGGIIDLY FAG +P  V+DQYT+LIGRPAP+PYW+FGFHQCR+
Sbjct: 280 NGMDVEYTGDRITYKVIGGIIDLYIFAGRTPEMVLDQYTKLIGRPAPMPYWAFGFHQCRW 339

Query: 338 GYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFPSDKMKIFVDNLHKN 397
           GY +V++IE+VV +YA+A IPLE MWTDIDYMD +KDFT DP++FP DKM+ FV  LH+N
Sbjct: 340 GYRDVNEIETVVDKYAEARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKLHRN 399

Query: 398 GQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETF 457
           GQ+YV ILDPGI+TN +YG +IRG ++++F+K NG PYLG VWPGPVY+PDF  P + +F
Sbjct: 400 GQRYVPILDPGINTNKSYGTFIRGMQSNVFIKRNGNPYLGSVWPGPVYYPDFLDPAARSF 459

Query: 458 WGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPPYMINNARVQRPLNNKTVP 517
           W  EI+ FRDI+P DG+WIDMNE SNFITS+  P S LDNPPY INN+  + P+N+KT+P
Sbjct: 460 WVDEIKRFRDILPIDGIWIDMNEASNFITSAPTPGSTLDNPPYKINNSGGRVPINSKTIP 519

Query: 518 ASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDI 577
           A+ +H+GN+ EYN HNLYGFLES+AT  +LV+   + PF+LSRSTF GSGKYTAHWTGD 
Sbjct: 520 ATAMHYGNVTEYNAHNLYGFLESQATREALVRPATRGPFLLSRSTFAGSGKYTAHWTGDN 579

Query: 578 GATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDK 637
            A W+DL Y+IP++LNFGLFG+PM+G+DICGF+  TTEELC RWIQLGAFYPF+RDHS +
Sbjct: 580 AARWDDLQYSIPTMLNFGLFGMPMIGADICGFAESTTEELCCRWIQLGAFYPFSRDHSAR 639

Query: 638 GSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTY 697
            +  QELYLW+SVAASAR VL LRY+LLPY+YTLMY+A+ +G+PIARPL F+FP D+ TY
Sbjct: 640 DTTHQELYLWESVAASARTVLGLRYELLPYYYTLMYDANLRGSPIARPLSFTFPDDVATY 699

Query: 698 EIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADH 757
            I SQFL+G G++VSPVL+ G+  V AY P GNW SL NY+  V+V++G  ++L AP DH
Sbjct: 700 GISSQFLIGRGIMVSPVLQPGSSIVNAYSPRGNWVSLSNYTSSVSVSAGTYVSLSAPPDH 759

Query: 758 INVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGN 817
           INVH+ EGNI+A+ GEAMTT+AA+ TP+ LLVV+S+  +S GE+FLD+G  +++G  GG 
Sbjct: 760 INVHIHEGNIVAMQGEAMTTQAARSTPFHLLVVMSDHVASTGELFLDNGIEMDIGGPGGK 819

Query: 818 WSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERPKKMVDLGLNISKGLN 877
           W++VRF++E+  + L + S+V+N G+A+SQ+ ++DK+T +G +R  K+ +  +    G  
Sbjct: 820 WTLVRFFAESGINNLTISSEVVNRGYAMSQRWVMDKITILGLKRRVKIKEYTVQKDAGAI 879

Query: 878 LNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFILEM 903
                  R +      F  + IS L   + + F LE+
Sbjct: 880 KVKGLGRRTSSHNQGGFFVSVISDLRQLVGQAFKLEL 906

BLAST of Chy12G210650 vs. ExPASy Swiss-Prot
Match: Q653V7 (Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0675700 PE=1 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 3.7e-288
Identity = 492/895 (54.97%), Postives = 648/895 (72.40%), Query Frame = 0

Query: 20  LILFLFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTADLDLI----GTSQVYGPDLP 79
           L +FL   FL  P      V  GY + S  V  +   L A L+L     G +   GPD+ 
Sbjct: 16  LAVFLLALFLAAPW----GVDCGYNVAS--VAGSKNRLRARLELAGGGGGAAPELGPDVR 75

Query: 80  TLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLPENHVASPK---ASFI 139
            L+L A+ E+  RL VRITD+   RWE+P  +IPR S       P++ +A+ +      +
Sbjct: 76  RLSLTASLETDSRLHVRITDADHPRWEVPQDVIPRPS-------PDSFLAATRPGGGRVL 135

Query: 140 SDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSL-PK 199
           S   SDL F +H T+PF F+V RRS+GDVLFDT+P+       LVFKD+Y++L+SSL P 
Sbjct: 136 STATSDLTFAIH-TSPFRFTVTRRSTGDVLFDTTPN-------LVFKDRYLELTSSLPPP 195

Query: 200 DRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGK 259
            R+S++G+GEQT+++F+L   +N T TLWN+DI + N+D+NLYG+HPFY+D+RS    G 
Sbjct: 196 GRASLYGLGEQTKRTFRL--QRNDTFTLWNSDIAAGNVDLNLYGSHPFYMDVRSGGGGG- 255

Query: 260 VAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 319
              G  HGVLLLNSNGMD+IY G  +TYKVIGG++D YFFAGPSP++V+DQYT+LIGRPA
Sbjct: 256 --GGAAHGVLLLNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVDQYTQLIGRPA 315

Query: 320 PVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFP 379
           P+PYWSFGFHQCRYGY NV+D+E VVA YAKA IPLE MWTDIDYMD YKDFT DP+NFP
Sbjct: 316 PMPYWSFGFHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYKDFTLDPVNFP 375

Query: 380 SDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGDVWPGP 439
           +D+M+ FVD LH+NGQK+V+I+DPGI+ N TYG ++RG + DIF+K+NG  YLG VWPG 
Sbjct: 376 ADRMRPFVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGSNYLGVVWPGN 435

Query: 440 VYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPPYMIN 499
           VYFPDF +P +  FW REI  FR  +P DGLW+DMNEISNF+     PL+ +D+PPY IN
Sbjct: 436 VYFPDFLNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFV--DPPPLNAIDDPPYRIN 495

Query: 500 NARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTF 559
           N+ V+RP+NNKTVPAS +H+G +AEY+ HNL+GFLE+RATH +L++ TG+RPFVLSRSTF
Sbjct: 496 NSGVRRPINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGRRPFVLSRSTF 555

Query: 560 VGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQ 619
           VGSG+YTAHWTGD  ATW DL Y+I ++L+FGLFGIPM+G+DICGF G+TTEELC RWIQ
Sbjct: 556 VGSGRYTAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNTTEELCSRWIQ 615

Query: 620 LGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIA 679
           LGAFYPF+RDHS  G++R+ELYLW+SVA SARK L LRY+LLPY YTLMYEAH  G PIA
Sbjct: 616 LGAFYPFSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMYEAHTTGAPIA 675

Query: 680 RPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEFVAV 739
           RPLFFS+P D++TY ID QFLLG GVLVSPVL+ GA +V AYFPAG WFSL+++S  VA 
Sbjct: 676 RPLFFSYPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFSLYDFSLAVAT 735

Query: 740 NSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVFL 799
            +G+++ L APAD +NVHV  GNIL L   A+T+   +++   LLV +++  ++ G++FL
Sbjct: 736 KTGKRVTLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALADDGTATGDLFL 795

Query: 800 DDGEVVEMGREGGNWSMVRF--YSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFER 859
           DDGE  EM      WS ++F   +E+ G  + V+S V++  +A S+ + I KV  +G   
Sbjct: 796 DDGESPEMAGPRSRWSQIKFSGATESGGGVVRVRSHVVHDSYAPSRTMAIGKVVLMGLRS 855

Query: 860 PKKMVDLGLNISKGLNLNGNSSIRKTYQYFAK--FMNAEISGLSIPIWEEFILEM 903
                   +  + G+ +N ++++        K     A +SGL++ + +EF L++
Sbjct: 856 AAPPKGFAV-YANGVQVNASTAVGGAAGSPEKGALGVAHVSGLTLVVGQEFDLKV 881

BLAST of Chy12G210650 vs. ExPASy Swiss-Prot
Match: Q43763 (Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 2.7e-275
Identity = 463/837 (55.32%), Postives = 604/837 (72.16%), Query Frame = 0

Query: 73  DLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRS-NSLIRSLPENHVASPKASF 132
           D+  L + A+ E+  RLRVRITD+   RWE+P  IIPR +   ++   P    A  +   
Sbjct: 59  DVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPGDVLHDAPPASSAPLQGRV 118

Query: 133 ISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLPK 192
           +S   SDL+ T+H  +PF F+V RRS+GD LFDT+P        LVF+D+Y++++S+LP 
Sbjct: 119 LSPAGSDLVLTVH-ASPFRFTVSRRSTGDTLFDTAPG-------LVFRDKYLEVTSALPA 178

Query: 193 DRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGK 252
            R+S++G+GE T+ SF+L    N + TLWNADIG+  +DVNLYG+HPFY+D+R+P     
Sbjct: 179 GRASLYGLGEHTKSSFRL--RHNDSFTLWNADIGASYVDVNLYGSHPFYMDVRAP----- 238

Query: 253 VAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 312
              GT HGVLLL+SNGMD++Y G  +TYKVIGG++D YFFAGP+P++V+DQYT+LI RPA
Sbjct: 239 ---GTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIARPA 298

Query: 313 PVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFP 372
           P+PYWSFGFHQCRYGY NVSD+E VVARYAKA IPLE MWTDIDYMDG+KDFT D +NF 
Sbjct: 299 PMPYWSFGFHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLDRVNFT 358

Query: 373 SDKMKIFVDNLHKNGQKYVLILDPGIST---NNTYGPYIRGTEADIFMKYNGVPYLGDVW 432
           + +++ FVD LH+N QKYVLILDPGI     + TYG ++RG + DIF+K NG  ++G+VW
Sbjct: 359 AAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKRNGTNFVGNVW 418

Query: 433 PGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPPY 492
           PG VYFPDF HP +  FW REI +FR  +P DGLWIDMNEISNF   +  P++ LD+PPY
Sbjct: 419 PGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFY--NPEPMNALDDPPY 478

Query: 493 MINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLSR 552
            INN    RP+NNKTV    +H+G + EY  HNL+G LE+RAT   +++ TG+RPFVLSR
Sbjct: 479 RINNDGTGRPINNKTVRPLAVHYGGVTEYEEHNLFGLLEARATGRGVLRDTGRRPFVLSR 538

Query: 553 STFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 612
           STFVGSG+YTA+WTGD  ATW DL Y+I ++L+FGLFG+PM+G+DICGF+G+TTEELC R
Sbjct: 539 STFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELCGR 598

Query: 613 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 672
           WIQLGAFYPF+RDHS   ++R+ELYLW SVAAS RK L LRYQLLPYFYTLMYEAH  G 
Sbjct: 599 WIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASGRKALGLRYQLLPYFYTLMYEAHMTGA 658

Query: 673 PIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEF 732
           PIARPLFFS+P D+ TY +D QFLLG GVLVSPVL+ G  +V+AYFPAG W+ L++YS  
Sbjct: 659 PIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGPTTVDAYFPAGRWYRLYDYSLA 718

Query: 733 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGE 792
           VA  +G+ + L APAD +NVH+  G IL L   A+TT  A+ T + LLV ++   ++ G 
Sbjct: 719 VATRTGKHVRLPAPADTVNVHLTGGTILPLQQSALTTSRARRTAFHLLVALAEDGTASGY 778

Query: 793 VFLDDGEVVEMGREGGNWSMVRFYSETVGSK--LVVKSQVINGGFALSQKLIIDKVTFVG 852
           +FLDDG+  E GR   +WSMVRF  +   +K  + VKS+V++  +A S+ L+I KV  +G
Sbjct: 779 LFLDDGDSPEYGRR-SDWSMVRFNYKIPNNKGAIKVKSEVVHNSYAQSRTLVISKVVLMG 838

Query: 853 FERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMN-AEISGLSIPIWEEFILEM 903
              P     L ++++    +  +SS    YQ        A I GLS+ + EEF L++
Sbjct: 839 HRSPAAPKKLTVHVNSA-EVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFELKV 873

BLAST of Chy12G210650 vs. ExPASy Swiss-Prot
Match: Q9S7Y7 (Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 1.2e-233
Identity = 417/932 (44.74%), Postives = 591/932 (63.41%), Query Frame = 0

Query: 1   MASGSSKVIRTPFPHSLLILILFLFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTAD 60
           MAS SS +    F  SLL+ ++  F+     P  S   +G GYR+ S    P G      
Sbjct: 1   MASSSSSL---AFSLSLLLALILCFS-----PTQSYKTIGKGYRLVSIEESPDG-GFIGY 60

Query: 61  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLP 120
           L +   +++YG D+ TL L    E+  RLRV ITD+ ++RWE+P++++PR     +  + 
Sbjct: 61  LQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVI 120

Query: 121 ENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKD 180
                SP  +      S+LIF+ + T PF F+V RRS+ + LF+T+       + LVFKD
Sbjct: 121 GKSRKSP-ITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTT-------SSLVFKD 180

Query: 181 QYIQLSSSLPKDRSSIFGIGEQTR-KSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPF 240
           QY+++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ ++NL+ +LYG+HP 
Sbjct: 181 QYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTDLYGSHPM 240

Query: 241 YIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISV 300
           Y+DLR+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF AGPSP++V
Sbjct: 241 YMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNV 300

Query: 301 IDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDG 360
           +DQYT+LIGRPAP+PYWS GFHQCR+GY+N+S +E VV  Y KA IPL+ +W D D+MDG
Sbjct: 301 VDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDG 360

Query: 361 YKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYN 420
           +KDFT +P+ +P  K+  F+D +HK G KY++I DPGI  N +YG + R   AD+F+KY 
Sbjct: 361 HKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYE 420

Query: 421 GVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFIT----- 480
           G P+L  VWPGPVYFPDF +P + ++WG EI+ F D+VP DGLWIDMNE+SNF +     
Sbjct: 421 GKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTI 480

Query: 481 -------SSTNP------------LSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMA 540
                  S   P             +  D+PPY IN   V  P+  KT+  S  H+  + 
Sbjct: 481 PEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVR 540

Query: 541 EYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYT 600
           EY+ H++YGF E+ ATH  L+ V G+RPF+LSRSTFVGSG+Y AHWTGD   TW  L  +
Sbjct: 541 EYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVS 600

Query: 601 IPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLW 660
           I ++LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+RDH++  S RQELY W
Sbjct: 601 ISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQW 660

Query: 661 DSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGG 720
           D+VA SAR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + Y    QFLLG 
Sbjct: 661 DTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGS 720

Query: 721 GVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNI 780
             ++SPVL++G   VEA FP G+W+ +F+ ++ V   +G+++ L AP + +NVH+ +  I
Sbjct: 721 SFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTI 780

Query: 781 LALHGEAMTTRAAQETPYKLLVVISNGQS---SFGEVFLDDGEVVEMGREGGNWSMVRFY 840
           L      + ++ A+ TP+ L++    G S   + G+++LD+ E+ EM    G  + V FY
Sbjct: 781 LPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFY 840

Query: 841 SETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERPKKMVDLGLNISKGLNLNGNSSI 899
           +      + + SQV  G FALS+  +I+KV+ +G     ++ ++ +N S        SS 
Sbjct: 841 ASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSK 900

BLAST of Chy12G210650 vs. ExPASy TrEMBL
Match: A0A0A0M3Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G716250 PE=3 SV=1)

HSP 1 Score: 1812.0 bits (4692), Expect = 0.0e+00
Identity = 883/906 (97.46%), Postives = 895/906 (98.79%), Query Frame = 0

Query: 1   MASGSSKVIRTPFPHSLLILILFLFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTAD 60
           MASGSSKVIRTPFPHSL ILILFLFTSFLPLPA SLPAVG GYRIRSSHVDPAGK+LTAD
Sbjct: 15  MASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIRSSHVDPAGKTLTAD 74

Query: 61  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLP 120
           LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWE+PHHI+PR S+SLIRSLP
Sbjct: 75  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHHILPRPSSSLIRSLP 134

Query: 121 ENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKD 180
           ENHVASPKASFIS PASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSP FSDSETFLVFKD
Sbjct: 135 ENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKD 194

Query: 181 QYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFY 240
           QYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFY
Sbjct: 195 QYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFY 254

Query: 241 IDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVI 300
           IDLRSPSQDGKVAAGTTHGVLLLNSNGMDI+YSGDRITYKVIGGIIDLYFFAGPSPISV+
Sbjct: 255 IDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGIIDLYFFAGPSPISVV 314

Query: 301 DQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGY 360
           DQYTELIGRPAPVPYWSFGFHQCRYGY NVSDIESVVARYAKASIPLEAMWTDIDYMDGY
Sbjct: 315 DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGY 374

Query: 361 KDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNG 420
           KDFTFDPINFPS KMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGT+ADIFMKYNG
Sbjct: 375 KDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYNG 434

Query: 421 VPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPL 480
           VPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST+PL
Sbjct: 435 VPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPL 494

Query: 481 SNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTG 540
           SNLDNPPYMINNARVQRPLNNKTVPASILHFGN+ EYNTHNLYGFLESRATHASLVKVTG
Sbjct: 495 SNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTG 554

Query: 541 QRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGD 600
           QRPFVLSRSTFVGSGKYTAHWTGD GATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGD
Sbjct: 555 QRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGD 614

Query: 601 TTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLM 660
           TTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLM
Sbjct: 615 TTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLM 674

Query: 661 YEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWF 720
           YEAHKKGTPIARPLFFSFPQDIKT+EIDSQFLLGGGVLVSPVLKEGAFSV+AYFPAGNWF
Sbjct: 675 YEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWF 734

Query: 721 SLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVIS 780
           SLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVIS
Sbjct: 735 SLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVIS 794

Query: 781 NGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIID 840
           NGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIID
Sbjct: 795 NGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIID 854

Query: 841 KVTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFIL 900
           KVTFVGF+RPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMN EISGLSIPIWEEFIL
Sbjct: 855 KVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFIL 914

Query: 901 EMTPIN 907
           EMTPIN
Sbjct: 915 EMTPIN 920

BLAST of Chy12G210650 vs. ExPASy TrEMBL
Match: A0A5D3E1Q3 (Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00330 PE=3 SV=1)

HSP 1 Score: 1749.9 bits (4531), Expect = 0.0e+00
Identity = 857/909 (94.28%), Postives = 881/909 (96.92%), Query Frame = 0

Query: 1   MASGSSK--VIRTPFPHSLLILILFLFTSFL-PLPATSLPAVGFGYRIRSSHVDPAGKSL 60
           MASGSSK   IRTPF HSLLILILFLFTSFL P PA SLPAVGFGYRIRSSH+DPAGKSL
Sbjct: 1   MASGSSKAITIRTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSL 60

Query: 61  TADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIR 120
           TADL LI TSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIP HIIPR SNSLIR
Sbjct: 61  TADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIR 120

Query: 121 SLPENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLV 180
           SLPENHVASPK SFISDPASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSPDFS+SETF+V
Sbjct: 121 SLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIV 180

Query: 181 FKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240
           FKDQYIQLSS LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH
Sbjct: 181 FKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240

Query: 241 PFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300
           PFYID+RS S+DGKV AGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI
Sbjct: 241 PFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300

Query: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYM 360
           SVIDQYTELIGRPAPVPYWSFGFHQCRYGY NVS++ESVVARYAKASIPLE MWTDIDYM
Sbjct: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYM 360

Query: 361 DGYKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMK 420
           DGYKDFTFDPINFPS+KMKIFVDNLHKNGQKYV+ILDPGISTNNTYGPYIRGT+ADIFMK
Sbjct: 361 DGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMK 420

Query: 421 YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480
           ++GVPYLGDVWPGPVYFPDF HPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST
Sbjct: 421 HDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480

Query: 481 NPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVK 540
           +PLSNLDNPPYMINNARV+RPLNNKTVPASILHFGN+ EYNTHNLYGFLESRATHASLVK
Sbjct: 481 SPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 540

Query: 541 VTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGF 600
           VTG+RPFVLSRSTF GSGKYTAHWTGDIGATWND+GYTIPSILNFGLFGIPMVGSDICGF
Sbjct: 541 VTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGF 600

Query: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660
           SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY
Sbjct: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660

Query: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAG 720
           TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLG GVLVSPVLKEGAFSV+AYFPAG
Sbjct: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAG 720

Query: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLV 780
           NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTT+AA+ET YKLLV
Sbjct: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLV 780

Query: 781 VISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKL 840
           VISNGQ SFGEVFLDDGEV EMG EGGNWSMVRF SETVGSKLVVKSQVINGGFALSQKL
Sbjct: 781 VISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKL 840

Query: 841 IIDKVTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEE 900
           IIDKVTFVGFERPKKMV LGLNISKG++LNGNSSIRKTYQYFAKFMN EISGLSIPIWEE
Sbjct: 841 IIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 900

Query: 901 FILEMTPIN 907
           FILEMTPI+
Sbjct: 901 FILEMTPIS 909

BLAST of Chy12G210650 vs. ExPASy TrEMBL
Match: A0A1S3C8V0 (alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1)

HSP 1 Score: 1749.9 bits (4531), Expect = 0.0e+00
Identity = 857/909 (94.28%), Postives = 881/909 (96.92%), Query Frame = 0

Query: 1   MASGSSK--VIRTPFPHSLLILILFLFTSFL-PLPATSLPAVGFGYRIRSSHVDPAGKSL 60
           MASGSSK   IRTPF HSLLILILFLFTSFL P PA SLPAVGFGYRIRSSH+DPAGKSL
Sbjct: 1   MASGSSKAITIRTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSL 60

Query: 61  TADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIR 120
           TADL LI TSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIP HIIPR SNSLIR
Sbjct: 61  TADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIR 120

Query: 121 SLPENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLV 180
           SLPENHVASPK SFISDPASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSPDFS+SETF+V
Sbjct: 121 SLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIV 180

Query: 181 FKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240
           FKDQYIQLSS LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH
Sbjct: 181 FKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240

Query: 241 PFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300
           PFYID+RS S+DGKV AGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI
Sbjct: 241 PFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300

Query: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYM 360
           SVIDQYTELIGRPAPVPYWSFGFHQCRYGY NVS++ESVVARYAKASIPLE MWTDIDYM
Sbjct: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYM 360

Query: 361 DGYKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMK 420
           DGYKDFTFDPINFPS+KMKIFVDNLHKNGQKYV+ILDPGISTNNTYGPYIRGT+ADIFMK
Sbjct: 361 DGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMK 420

Query: 421 YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480
           ++GVPYLGDVWPGPVYFPDF HPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST
Sbjct: 421 HDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480

Query: 481 NPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVK 540
           +PLSNLDNPPYMINNARV+RPLNNKTVPASILHFGN+ EYNTHNLYGFLESRATHASLVK
Sbjct: 481 SPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 540

Query: 541 VTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGF 600
           VTG+RPFVLSRSTF GSGKYTAHWTGDIGATWND+GYTIPSILNFGLFGIPMVGSDICGF
Sbjct: 541 VTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGF 600

Query: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660
           SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY
Sbjct: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660

Query: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAG 720
           TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLG GVLVSPVLKEGAFSV+AYFPAG
Sbjct: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAG 720

Query: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLV 780
           NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTT+AA+ET YKLLV
Sbjct: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLV 780

Query: 781 VISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKL 840
           VISNGQ SFGEVFLDDGEV EMG EGGNWSMVRF SETVGSKLVVKSQVINGGFALSQKL
Sbjct: 781 VISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKL 840

Query: 841 IIDKVTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEE 900
           IIDKVTFVGFERPKKMV LGLNISKG++LNGNSSIRKTYQYFAKFMN EISGLSIPIWEE
Sbjct: 841 IIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 900

Query: 901 FILEMTPIN 907
           FILEMTPI+
Sbjct: 901 FILEMTPIS 909

BLAST of Chy12G210650 vs. ExPASy TrEMBL
Match: A0A6J1KFK1 (alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1)

HSP 1 Score: 1577.4 bits (4083), Expect = 0.0e+00
Identity = 766/900 (85.11%), Postives = 826/900 (91.78%), Query Frame = 0

Query: 17  LLILILFLFTSFLPLPATSLP----------AVGFGYRIRSSHVDPAGKSLTADLDLIGT 76
           L  ++LFLF+ F  L A SLP          AVG+GYR+RS  VDP GKSLTADLDLI  
Sbjct: 39  LSAVVLFLFSCFFRLIA-SLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEK 98

Query: 77  SQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLPENHVAS 136
           S VYGPD+  L+LQA+FE+KDRLRVRITDS RERWEIP  IIPRRSNS IRSLPE  V S
Sbjct: 99  SSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGS 158

Query: 137 PKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLS 196
           P+ S ISDPASDLIF+LHDTAPFGFSV RRSSGDVLFDTSP+FSDSETFLVFKDQYIQLS
Sbjct: 159 PETSIISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLS 218

Query: 197 SSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSP 256
           SSLPKDRSS+FGIGEQTRKSFK+VPDK+KTLTLW+ADIGSVN DVNLYGAHPFYID+RSP
Sbjct: 219 SSLPKDRSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSP 278

Query: 257 SQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTEL 316
           S+DGKVAAGTTHGVLLLNSNGMDIIYSGDRI+YK IGGIIDLYFFAGPSPISVIDQYTEL
Sbjct: 279 SRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTEL 338

Query: 317 IGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFD 376
           IGRPAPVPYWSFGFHQCRYGY NVSDIE+VVARYAKA IPLE MWTDIDYMDGYKDFTFD
Sbjct: 339 IGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFD 398

Query: 377 PINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGD 436
           PINFP +KMK FVD+LH+NGQKYVLI+DPGISTN TYG YIRG EADIF+KY+GVPYLG+
Sbjct: 399 PINFPVEKMKNFVDSLHQNGQKYVLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGE 458

Query: 437 VWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNP 496
           VWPGPVYFPDF HP+SE FWG EI++FRDIVPFDGLWIDMNE+SNFITSST+P SNLDNP
Sbjct: 459 VWPGPVYFPDFLHPNSEIFWGGEIKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNP 518

Query: 497 PYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVL 556
           PY I+NA VQRP+NN+TVPAS LHFGN+ EYNTHNLYGFLES+ATHASLVKVTG+RPFVL
Sbjct: 519 PYKIDNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVL 578

Query: 557 SRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELC 616
           SRSTFVGSGKYTAHWTGD  ATWNDLGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELC
Sbjct: 579 SRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELC 638

Query: 617 RRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKK 676
           RRWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY LLPY YTLMYEAHKK
Sbjct: 639 RRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKK 698

Query: 677 GTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYS 736
           GTPIARPLFFSFPQDI+TYEI+SQFLLG GVLVSPVLKEGA SV+AYFPAGNWFSLFNYS
Sbjct: 699 GTPIARPLFFSFPQDIRTYEINSQFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYS 758

Query: 737 EFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSF 796
           E VAV SGQQI LDAPADHINVHVREGNILALHGEA TT+AA++T +KLLVV+SNGQSS 
Sbjct: 759 ESVAVKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSS 818

Query: 797 GEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVG 856
           GEVFLDDGEVVEMG EGGNWS+VRFYSE VGSKL++KSQVINGGFALSQK+IIDKVTFVG
Sbjct: 819 GEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVG 878

Query: 857 FERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFILEMTPIN 907
           FERPKKM  LGL+ISKG NLNGNS IRKTY+Y AKF+N EISGLSIPI EEF++E++P+N
Sbjct: 879 FERPKKMGGLGLDISKGANLNGNSGIRKTYEYSAKFVNVEISGLSIPICEEFVMELSPLN 937

BLAST of Chy12G210650 vs. ExPASy TrEMBL
Match: A0A6J1ENT6 (alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1)

HSP 1 Score: 1566.2 bits (4054), Expect = 0.0e+00
Identity = 760/900 (84.44%), Postives = 819/900 (91.00%), Query Frame = 0

Query: 17  LLILILFLFTSFLPLPATSLP----------AVGFGYRIRSSHVDPAGKSLTADLDLIGT 76
           L  ++LFLFT F  L A SLP          AVG+GYR+RS  VDP GKSLTADLDLI  
Sbjct: 18  LSAVVLFLFTCFFRLTA-SLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIDK 77

Query: 77  SQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLPENHVAS 136
           S VYGPD+  L+LQA+FE+KDRLRVRITDS RERWE+P  IIPRRSNS IRSLPE  V S
Sbjct: 78  SSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGS 137

Query: 137 PKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLS 196
           P+ S ISDPASDLIF+LHDTAPFGFSV RRSSGDVLFDTSP+FSDSETFLVFKDQYIQLS
Sbjct: 138 PETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLS 197

Query: 197 SSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSP 256
           SSLPKDRSS+FGIGEQTRKSFKLVPDK+KTLTLWNADIGSVN DVNLYGAHPFYID+RSP
Sbjct: 198 SSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSP 257

Query: 257 SQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTEL 316
           S+DGKVAAGTTHGVLLLNSNGMDIIY+GDRI+YK IGGIIDLYFFAGPSPISVIDQYTEL
Sbjct: 258 SRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTEL 317

Query: 317 IGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFD 376
           IGRPAPVPYWSFGFHQCRYGY NVSDIE+VVARYAKA IPLE MWTDIDYMDGYKDFTFD
Sbjct: 318 IGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFD 377

Query: 377 PINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGD 436
           PINFP +KMK FVDNLH+NGQKYVLILDPGISTN TYG YIRG EADIF+KY+GVPYLG+
Sbjct: 378 PINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGE 437

Query: 437 VWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNP 496
           VWPGPVYFPDF HP+SE FWG EI++ RDIVPFDGLWIDMNEISNFITSST+  SNLDNP
Sbjct: 438 VWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNP 497

Query: 497 PYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVL 556
           PY INNA VQRP+NN+TVPAS LHFGN+ EYNTHNLYGFLES+ATHASLVKVTG+RPFVL
Sbjct: 498 PYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVL 557

Query: 557 SRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELC 616
           SRSTFVGSGKYTAHWTGD  ATWNDLGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELC
Sbjct: 558 SRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELC 617

Query: 617 RRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKK 676
           RRWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY LLPY YTLMYEAHKK
Sbjct: 618 RRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKK 677

Query: 677 GTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYS 736
           GTPIARPLFFSFPQDI+TYEI+SQFLLG GVL+SPVLKEGA SV+AYFPAGNWFSLFNYS
Sbjct: 678 GTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYS 737

Query: 737 EFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSF 796
           E VA+ SGQQI LDAPADHINVHVREGNILALHGEA TT+AA++T +KLLVV+SNGQSS 
Sbjct: 738 ESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSSS 797

Query: 797 GEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVG 856
           GEVFLDDGE +EMG EGGNWS+VRFYSE VGSKL++KSQVINGGFALSQK+IIDKVT VG
Sbjct: 798 GEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVG 857

Query: 857 FERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFILEMTPIN 907
           FERPK M DLGL+ISKG NLNGNS IR TY+Y AKF+N +ISGLSIPI E F++E++ +N
Sbjct: 858 FERPKNMGDLGLDISKGANLNGNSGIRITYEYSAKFVNVQISGLSIPICEGFVMELSSLN 916

BLAST of Chy12G210650 vs. NCBI nr
Match: XP_011660330.1 (alpha-glucosidase [Cucumis sativus] >KGN66921.1 hypothetical protein Csa_007658 [Cucumis sativus])

HSP 1 Score: 1801 bits (4664), Expect = 0.0
Identity = 883/906 (97.46%), Postives = 895/906 (98.79%), Query Frame = 0

Query: 1   MASGSSKVIRTPFPHSLLILILFLFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTAD 60
           MASGSSKVIRTPFPHSL ILILFLFTSFLPLPA SLPAVG GYRIRSSHVDPAGK+LTAD
Sbjct: 15  MASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIRSSHVDPAGKTLTAD 74

Query: 61  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLP 120
           LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWE+PHHI+PR S+SLIRSLP
Sbjct: 75  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHHILPRPSSSLIRSLP 134

Query: 121 ENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKD 180
           ENHVASPKASFIS PASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSP FSDSETFLVFKD
Sbjct: 135 ENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKD 194

Query: 181 QYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFY 240
           QYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFY
Sbjct: 195 QYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFY 254

Query: 241 IDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVI 300
           IDLRSPSQDGKVAAGTTHGVLLLNSNGMDI+YSGDRITYKVIGGIIDLYFFAGPSPISV+
Sbjct: 255 IDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGIIDLYFFAGPSPISVV 314

Query: 301 DQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGY 360
           DQYTELIGRPAPVPYWSFGFHQCRYGY NVSDIESVVARYAKASIPLEAMWTDIDYMDGY
Sbjct: 315 DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGY 374

Query: 361 KDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNG 420
           KDFTFDPINFPS KMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGT+ADIFMKYNG
Sbjct: 375 KDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYNG 434

Query: 421 VPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPL 480
           VPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST+PL
Sbjct: 435 VPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPL 494

Query: 481 SNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTG 540
           SNLDNPPYMINNARVQRPLNNKTVPASILHFGN+ EYNTHNLYGFLESRATHASLVKVTG
Sbjct: 495 SNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTG 554

Query: 541 QRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGD 600
           QRPFVLSRSTFVGSGKYTAHWTGD GATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGD
Sbjct: 555 QRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGD 614

Query: 601 TTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLM 660
           TTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLM
Sbjct: 615 TTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLM 674

Query: 661 YEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWF 720
           YEAHKKGTPIARPLFFSFPQDIKT+EIDSQFLLGGGVLVSPVLKEGAFSV+AYFPAGNWF
Sbjct: 675 YEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWF 734

Query: 721 SLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVIS 780
           SLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVIS
Sbjct: 735 SLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVIS 794

Query: 781 NGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIID 840
           NGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIID
Sbjct: 795 NGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIID 854

Query: 841 KVTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFIL 900
           KVTFVGF+RPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMN EISGLSIPIWEEFIL
Sbjct: 855 KVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFIL 914

Query: 901 EMTPIN 906
           EMTPIN
Sbjct: 915 EMTPIN 920

BLAST of Chy12G210650 vs. NCBI nr
Match: XP_008459069.1 (PREDICTED: alpha-glucosidase [Cucumis melo] >KAA0043261.1 alpha-glucosidase [Cucumis melo var. makuwa] >TYK29325.1 alpha-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 1739 bits (4503), Expect = 0.0
Identity = 857/909 (94.28%), Postives = 881/909 (96.92%), Query Frame = 0

Query: 1   MASGSSKVI--RTPFPHSLLILILFLFTSFL-PLPATSLPAVGFGYRIRSSHVDPAGKSL 60
           MASGSSK I  RTPF HSLLILILFLFTSFL P PA SLPAVGFGYRIRSSH+DPAGKSL
Sbjct: 1   MASGSSKAITIRTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSL 60

Query: 61  TADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIR 120
           TADL LI TSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIP HIIPR SNSLIR
Sbjct: 61  TADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIR 120

Query: 121 SLPENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLV 180
           SLPENHVASPK SFISDPASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSPDFS+SETF+V
Sbjct: 121 SLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIV 180

Query: 181 FKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240
           FKDQYIQLSS LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH
Sbjct: 181 FKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240

Query: 241 PFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300
           PFYID+RS S+DGKV AGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI
Sbjct: 241 PFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300

Query: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYM 360
           SVIDQYTELIGRPAPVPYWSFGFHQCRYGY NVS++ESVVARYAKASIPLE MWTDIDYM
Sbjct: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYM 360

Query: 361 DGYKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMK 420
           DGYKDFTFDPINFPS+KMKIFVDNLHKNGQKYV+ILDPGISTNNTYGPYIRGT+ADIFMK
Sbjct: 361 DGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMK 420

Query: 421 YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480
           ++GVPYLGDVWPGPVYFPDF HPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST
Sbjct: 421 HDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480

Query: 481 NPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVK 540
           +PLSNLDNPPYMINNARV+RPLNNKTVPASILHFGN+ EYNTHNLYGFLESRATHASLVK
Sbjct: 481 SPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 540

Query: 541 VTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGF 600
           VTG+RPFVLSRSTF GSGKYTAHWTGDIGATWND+GYTIPSILNFGLFGIPMVGSDICGF
Sbjct: 541 VTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGF 600

Query: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660
           SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY
Sbjct: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660

Query: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAG 720
           TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLG GVLVSPVLKEGAFSV+AYFPAG
Sbjct: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAG 720

Query: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLV 780
           NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTT+AA+ET YKLLV
Sbjct: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLV 780

Query: 781 VISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKL 840
           VISNGQ SFGEVFLDDGEV EMG EGGNWSMVRF SETVGSKLVVKSQVINGGFALSQKL
Sbjct: 781 VISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKL 840

Query: 841 IIDKVTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEE 900
           IIDKVTFVGFERPKKMV LGLNISKG++LNGNSSIRKTYQYFAKFMN EISGLSIPIWEE
Sbjct: 841 IIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 900

Query: 901 FILEMTPIN 906
           FILEMTPI+
Sbjct: 901 FILEMTPIS 909

BLAST of Chy12G210650 vs. NCBI nr
Match: XP_038894574.1 (alpha-glucosidase [Benincasa hispida])

HSP 1 Score: 1628 bits (4215), Expect = 0.0
Identity = 804/913 (88.06%), Postives = 847/913 (92.77%), Query Frame = 0

Query: 1   MASGSSKVIRTPFPHSLL------ILILFLFTSFLP-LPATSLPAVGFGYRIRSSHVDPA 60
           M SGS K +RTPF  SL        L+ FLF SF P L A SL AVG+GYRIRSSHVDPA
Sbjct: 1   MGSGS-KAMRTPFSQSLQPPLLFPALLFFLFPSFFPNLVAGSLSAVGYGYRIRSSHVDPA 60

Query: 61  GKSLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSN 120
           GKSLTADLDLI TS V GPD+P LTLQATFE+KDRLR+RITDSTRERWEIP  IIPRRS+
Sbjct: 61  GKSLTADLDLIRTSPVNGPDVPRLTLQATFETKDRLRIRITDSTRERWEIPDQIIPRRSS 120

Query: 121 SLIRSLPENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSE 180
           S IRSLPENHV SP+ SFISDPASDLIFTLH TAPFGFSVLRRSSGDVLFDTSPD SDSE
Sbjct: 121 SRIRSLPENHVGSPRTSFISDPASDLIFTLHYTAPFGFSVLRRSSGDVLFDTSPDLSDSE 180

Query: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNL 240
           TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTR+SFKL+PDKNKTLTLWNADIGSVNLDVNL
Sbjct: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLMPDKNKTLTLWNADIGSVNLDVNL 240

Query: 241 YGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAG 300
           YGAHPFYID+RSPS DGKVAAGTTHGVLLLNSNGMDIIYSG RI YKVIGGIIDLYFFAG
Sbjct: 241 YGAHPFYIDVRSPSGDGKVAAGTTHGVLLLNSNGMDIIYSGHRIMYKVIGGIIDLYFFAG 300

Query: 301 PSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTD 360
           PSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGY NVSD+ESVVARYAKA IPLE MWTD
Sbjct: 301 PSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDLESVVARYAKAGIPLEVMWTD 360

Query: 361 IDYMDGYKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEAD 420
           IDYMDGYKDFTFDP+NFP++KMK FVDNLHKNGQKYVLILDPGISTNNTYG +IRG  AD
Sbjct: 361 IDYMDGYKDFTFDPVNFPAEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTFIRGKRAD 420

Query: 421 IFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFI 480
           IF++Y+GVPYLG+VWPGPVYFPDF HP+SE FWG EIQ+FRDIVPFDGLWIDMNEISNFI
Sbjct: 421 IFIQYDGVPYLGEVWPGPVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFI 480

Query: 481 TSSTNPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHA 540
           TSST+P SNLDNPPYMINNA V+RPLNNKTVPASILHFGN+ EYNTHNLYGFLES+ATHA
Sbjct: 481 TSSTSPFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESKATHA 540

Query: 541 SLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSD 600
           SLVKVTG+RPFVLSRSTFVGSGKYTAHWTGD GATWNDL  TIPSILNFGLFGIPMVG+D
Sbjct: 541 SLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLRNTIPSILNFGLFGIPMVGAD 600

Query: 601 ICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLL 660
           ICGFSGDTTEELC RWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY+LL
Sbjct: 601 ICGFSGDTTEELCGRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRLL 660

Query: 661 PYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAY 720
           PYFYTLMYEAHKKGTPIARPLFFSFPQDIKTY I+SQFLLG GVLVSPVLKEGA SV+AY
Sbjct: 661 PYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYGINSQFLLGEGVLVSPVLKEGAVSVDAY 720

Query: 721 FPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPY 780
           FP GNWFSLFNYSE VAV SGQ+I LDAPADHINVHVREGNILALHG+AMTTRAA+ET Y
Sbjct: 721 FPTGNWFSLFNYSELVAVKSGQRITLDAPADHINVHVREGNILALHGKAMTTRAARETAY 780

Query: 781 KLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFAL 840
           +LLVV+SN QSSFGEVFLDDGEVVEMG EGGNWS+VRFYSE VGSKLVVKSQVINGGFAL
Sbjct: 781 ELLVVVSNDQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEAVGSKLVVKSQVINGGFAL 840

Query: 841 SQKLIIDKVTFVGFERPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIP 900
           SQ LIIDKVTFVGFERPKKMVDLGLNISKG+ LNGNS+IRKTY+YFAK MN EISGLSIP
Sbjct: 841 SQNLIIDKVTFVGFERPKKMVDLGLNISKGVKLNGNSTIRKTYKYFAKVMNVEISGLSIP 900

Query: 901 IWEEFILEMTPIN 906
           IWEEF+LEMTPI+
Sbjct: 901 IWEEFMLEMTPIS 912

BLAST of Chy12G210650 vs. NCBI nr
Match: XP_023520110.1 (alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1568 bits (4061), Expect = 0.0
Identity = 765/896 (85.38%), Postives = 821/896 (91.63%), Query Frame = 0

Query: 21  ILFLFTSFLPLPATSLP----------AVGFGYRIRSSHVDPAGKSLTADLDLIGTSQVY 80
           +LFLFT F  L A SLP          AVG+GYR+RS  VDP GKSLTADLDLI  S VY
Sbjct: 43  VLFLFTCFFRLTA-SLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVY 102

Query: 81  GPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLPENHVASPKAS 140
           GPD+  L++QA+FE+KDRLRVRITDS RERWE+P  IIPRRSNS IRSLPE  V SP+ S
Sbjct: 103 GPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETS 162

Query: 141 FISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLP 200
            ISDPASDLIF+LHDTAPFGFSV RRSSGDVLFDTSP+FSDSETFLVFKDQYIQLSSSLP
Sbjct: 163 VISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLP 222

Query: 201 KDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDG 260
           KDRSS+FGIGEQTRKSFKLVPDK+KTLTLWNADIGSVN DVNLYGAHPFYID+RSPS+DG
Sbjct: 223 KDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDG 282

Query: 261 KVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRP 320
           KVAAGTTHGVLLLNSNGMDIIYSGDRI+YK IGGIIDLYFFAGPSPISVIDQYTELIGRP
Sbjct: 283 KVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRP 342

Query: 321 APVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINF 380
           APVPYWSFGFHQCRYGY NVSDIE+VVARYAKA IPLE MWTDIDYMDGYKDFTFDPINF
Sbjct: 343 APVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINF 402

Query: 381 PSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGDVWPG 440
           P +KMK FVDNLH+NGQKYVLILDPGISTN TYG YIRG EADIF+KY+GVPYLG+VWPG
Sbjct: 403 PVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPG 462

Query: 441 PVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPPYMI 500
           PVYFPDF HP+SE FWG EI++FRDIVPFDGLWIDMNEISNFITSST+  SNLDNPPY I
Sbjct: 463 PVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKI 522

Query: 501 NNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRST 560
           NNA VQRP+NN+TVPAS LHFGN+ EYNTHNLYGFLES+ATHASLVKVTG+RPFVLSRST
Sbjct: 523 NNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRST 582

Query: 561 FVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWI 620
           FVGSGKYTAHWTGD  ATWNDLGYTIPSILNFGLFGIPMVG+DICGFS DTTEELCRRWI
Sbjct: 583 FVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCRRWI 642

Query: 621 QLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPI 680
           QLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY LLPY YTLMYEAHKKGTPI
Sbjct: 643 QLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPI 702

Query: 681 ARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEFVA 740
           ARPLFFSFPQDI+TYEI+SQFLLG GVL+SPVLKEGA SV+AYFPAGNWFSLFNYSE VA
Sbjct: 703 ARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVA 762

Query: 741 VNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVF 800
           VNSGQQI LDAPADHINVHVREGNILALHGEA TT+AA++T +KLLVV+SNGQSS GEVF
Sbjct: 763 VNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVF 822

Query: 801 LDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERP 860
           LDDGEVVEMG EGGNWS+VRFYSE VGSKL++KSQVINGGFALSQK+IIDKVT VGFERP
Sbjct: 823 LDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERP 882

Query: 861 KKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFILEMTPIN 906
           K M  LGL+ISKG NL+GNS IRKTY+Y AKF+N EISGLSI IWEEF++E++P+N
Sbjct: 883 KNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN 937

BLAST of Chy12G210650 vs. NCBI nr
Match: XP_023001112.1 (alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1567 bits (4057), Expect = 0.0
Identity = 765/896 (85.38%), Postives = 824/896 (91.96%), Query Frame = 0

Query: 21  ILFLFTSFLPLPATSLP----------AVGFGYRIRSSHVDPAGKSLTADLDLIGTSQVY 80
           +LFLF+ F  L A SLP          AVG+GYR+RS  VDP GKSLTADLDLI  S VY
Sbjct: 43  VLFLFSCFFRLIA-SLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVY 102

Query: 81  GPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLPENHVASPKAS 140
           GPD+  L+LQA+FE+KDRLRVRITDS RERWEIP  IIPRRSNS IRSLPE  V SP+ S
Sbjct: 103 GPDVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETS 162

Query: 141 FISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLP 200
            ISDPASDLIF+LHDTAPFGFSV RRSSGDVLFDTSP+FSDSETFLVFKDQYIQLSSSLP
Sbjct: 163 IISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLP 222

Query: 201 KDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDG 260
           KDRSS+FGIGEQTRKSFK+VPDK+KTLTLW+ADIGSVN DVNLYGAHPFYID+RSPS+DG
Sbjct: 223 KDRSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSPSRDG 282

Query: 261 KVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRP 320
           KVAAGTTHGVLLLNSNGMDIIYSGDRI+YK IGGIIDLYFFAGPSPISVIDQYTELIGRP
Sbjct: 283 KVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRP 342

Query: 321 APVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINF 380
           APVPYWSFGFHQCRYGY NVSDIE+VVARYAKA IPLE MWTDIDYMDGYKDFTFDPINF
Sbjct: 343 APVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINF 402

Query: 381 PSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGDVWPG 440
           P +KMK FVD+LH+NGQKYVLI+DPGISTN TYG YIRG EADIF+KY+GVPYLG+VWPG
Sbjct: 403 PVEKMKNFVDSLHQNGQKYVLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPG 462

Query: 441 PVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPPYMI 500
           PVYFPDF HP+SE FWG EI++FRDIVPFDGLWIDMNE+SNFITSST+P SNLDNPPY I
Sbjct: 463 PVYFPDFLHPNSEIFWGGEIKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNPPYKI 522

Query: 501 NNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRST 560
           +NA VQRP+NN+TVPAS LHFGN+ EYNTHNLYGFLES+ATHASLVKVTG+RPFVLSRST
Sbjct: 523 DNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRST 582

Query: 561 FVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWI 620
           FVGSGKYTAHWTGD  ATWNDLGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELCRRWI
Sbjct: 583 FVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWI 642

Query: 621 QLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPI 680
           QLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY LLPY YTLMYEAHKKGTPI
Sbjct: 643 QLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPI 702

Query: 681 ARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEFVA 740
           ARPLFFSFPQDI+TYEI+SQFLLG GVLVSPVLKEGA SV+AYFPAGNWFSLFNYSE VA
Sbjct: 703 ARPLFFSFPQDIRTYEINSQFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYSESVA 762

Query: 741 VNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVF 800
           V SGQQI LDAPADHINVHVREGNILALHGEA TT+AA++T +KLLVV+SNGQSS GEVF
Sbjct: 763 VKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVF 822

Query: 801 LDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERP 860
           LDDGEVVEMG EGGNWS+VRFYSE VGSKL++KSQVINGGFALSQK+IIDKVTFVGFERP
Sbjct: 823 LDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVGFERP 882

Query: 861 KKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNAEISGLSIPIWEEFILEMTPIN 906
           KKM  LGL+ISKG NLNGNS IRKTY+Y AKF+N EISGLSIPI EEF++E++P+N
Sbjct: 883 KKMGGLGLDISKGANLNGNSGIRKTYEYSAKFVNVEISGLSIPICEEFVMELSPLN 937

BLAST of Chy12G210650 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 560/897 (62.43%), Postives = 700/897 (78.04%), Query Frame = 0

Query: 13  FPHSLLILILF--LFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTADLDLIGTSQVY 72
           FP+  +++++F  L +S + L       VG+GY +RS  VD   + LTA LDLI  S VY
Sbjct: 7   FPNIFIVVVVFFSLRSSQVVLEEEESTVVGYGYVVRSVGVDSNRQVLTAKLDLIKPSSVY 66

Query: 73  GPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIR--SLPENHVASPK 132
            PD+ +L L  + E+ +RLR+RITDS+++RWEIP  +IPR  N   R  S  E+   SP+
Sbjct: 67  APDIKSLNLHVSLETSERLRIRITDSSQQRWEIPETVIPRAGNHSPRRFSTEEDGGNSPE 126

Query: 133 ASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSS 192
            +F++DP+SDL+FTLH+T PFGFSV RRSSGD+LFDTSPD SDS T+ +FKDQ++QLSS+
Sbjct: 127 NNFLADPSSDLVFTLHNTTPFGFSVSRRSSGDILFDTSPDSSDSNTYFIFKDQFLQLSSA 186

Query: 193 LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQ 252
           LP++RS+++GIGE T++SF+L+P   +T+TLWNADIGS N DVNLYG+HPFY+D+R    
Sbjct: 187 LPENRSNLYGIGEHTKRSFRLIP--GETMTLWNADIGSENPDVNLYGSHPFYMDVR--GS 246

Query: 253 DGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIG 312
            G   AGTTHGVLLLNSNGMD+ Y G RITY VIGG+IDLY FAGPSP  V++QYTELIG
Sbjct: 247 KGNEEAGTTHGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGPSPEMVMNQYTELIG 306

Query: 313 RPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPI 372
           RPAP+PYWSFGFHQCRYGY NVSD+E VV  YAKA IPLE MWTDIDYMDGYKDFT DP+
Sbjct: 307 RPAPMPYWSFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPV 366

Query: 373 NFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGDVW 432
           NFP DKM+ FVD LHKNGQKYVLILDPGI  +++YG Y RG EAD+F+K NG PYLG+VW
Sbjct: 367 NFPEDKMQSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIKRNGEPYLGEVW 426

Query: 433 PGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPPY 492
           PG VYFPDF +P + TFW  EI++F++I+P DGLWIDMNE+SNFITS  +  S+LD+PPY
Sbjct: 427 PGKVYFPDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDPPY 486

Query: 493 MINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLSR 552
            INN+  +RP+NNKTVPA+ +HFGN++EY+ HNLYG LE++ATH ++V +TG+RPF+LSR
Sbjct: 487 KINNSGDKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDITGKRPFILSR 546

Query: 553 STFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 612
           STFV SGKYTAHWTGD  A W DL Y+IP ILNFGLFGIPMVG+DICGFS DTTEELCRR
Sbjct: 547 STFVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELCRR 606

Query: 613 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 672
           WIQLGAFYPFARDHS  G+ RQELYLWDSVA+SARKVL LR +LLP+ YTLMYEAH  G 
Sbjct: 607 WIQLGAFYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVSGN 666

Query: 673 PIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEF 732
           PIARPLFFSFPQD KTYEIDSQFL+G  ++VSP LK+GA +V+AYFPAGNWF LFNYS  
Sbjct: 667 PIARPLFFSFPQDTKTYEIDSQFLIGKSIMVSPALKQGAVAVDAYFPAGNWFDLFNYSFA 726

Query: 733 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGE 792
           V  +SG+ + LD PADH+NVHVREG+I+A+ GEA+TTR A++TPY+LLVV S  ++  GE
Sbjct: 727 VGGDSGKHVRLDTPADHVNVHVREGSIVAMQGEALTTRDARKTPYQLLVVASRLENISGE 786

Query: 793 VFLDDGEVVEMGREGGN--WSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVG 852
           +FLDDGE + MG  GGN  W++V+F     G  +V++S+V+N  +A   K  I KVTFVG
Sbjct: 787 LFLDDGENLRMGAGGGNRDWTLVKFRCYVTGKSVVLRSEVVNPEYASKMKWSIGKVTFVG 846

Query: 853 FERPKKMVDLGLNISKGLNLNGNSSIRKTYQY-FAKFMNAEISGLSIPIWEEFILEM 903
           FE  + +    +  S+ L     S I+        +F++ E+S LS+ + ++F + +
Sbjct: 847 FENVENVKTYEVRTSERLRSPRISLIKTVSDNDDPRFLSVEVSKLSLLVGKKFEMRL 899

BLAST of Chy12G210650 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 811.2 bits (2094), Expect = 8.3e-235
Identity = 417/932 (44.74%), Postives = 591/932 (63.41%), Query Frame = 0

Query: 1   MASGSSKVIRTPFPHSLLILILFLFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTAD 60
           MAS SS +    F  SLL+ ++  F+     P  S   +G GYR+ S    P G      
Sbjct: 1   MASSSSSL---AFSLSLLLALILCFS-----PTQSYKTIGKGYRLVSIEESPDG-GFIGY 60

Query: 61  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLP 120
           L +   +++YG D+ TL L    E+  RLRV ITD+ ++RWE+P++++PR     +  + 
Sbjct: 61  LQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVI 120

Query: 121 ENHVASPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKD 180
                SP  +      S+LIF+ + T PF F+V RRS+ + LF+T+       + LVFKD
Sbjct: 121 GKSRKSP-ITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTT-------SSLVFKD 180

Query: 181 QYIQLSSSLPKDRSSIFGIGEQTR-KSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPF 240
           QY+++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ ++NL+ +LYG+HP 
Sbjct: 181 QYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTDLYGSHPM 240

Query: 241 YIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISV 300
           Y+DLR+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF AGPSP++V
Sbjct: 241 YMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNV 300

Query: 301 IDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDG 360
           +DQYT+LIGRPAP+PYWS GFHQCR+GY+N+S +E VV  Y KA IPL+ +W D D+MDG
Sbjct: 301 VDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDG 360

Query: 361 YKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYN 420
           +KDFT +P+ +P  K+  F+D +HK G KY++I DPGI  N +YG + R   AD+F+KY 
Sbjct: 361 HKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYE 420

Query: 421 GVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFIT----- 480
           G P+L  VWPGPVYFPDF +P + ++WG EI+ F D+VP DGLWIDMNE+SNF +     
Sbjct: 421 GKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTI 480

Query: 481 -------SSTNP------------LSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMA 540
                  S   P             +  D+PPY IN   V  P+  KT+  S  H+  + 
Sbjct: 481 PEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVR 540

Query: 541 EYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYT 600
           EY+ H++YGF E+ ATH  L+ V G+RPF+LSRSTFVGSG+Y AHWTGD   TW  L  +
Sbjct: 541 EYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVS 600

Query: 601 IPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLW 660
           I ++LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+RDH++  S RQELY W
Sbjct: 601 ISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQW 660

Query: 661 DSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGG 720
           D+VA SAR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + Y    QFLLG 
Sbjct: 661 DTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGS 720

Query: 721 GVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNI 780
             ++SPVL++G   VEA FP G+W+ +F+ ++ V   +G+++ L AP + +NVH+ +  I
Sbjct: 721 SFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTI 780

Query: 781 LALHGEAMTTRAAQETPYKLLVVISNGQS---SFGEVFLDDGEVVEMGREGGNWSMVRFY 840
           L      + ++ A+ TP+ L++    G S   + G+++LD+ E+ EM    G  + V FY
Sbjct: 781 LPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFY 840

Query: 841 SETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERPKKMVDLGLNISKGLNLNGNSSI 899
           +      + + SQV  G FALS+  +I+KV+ +G     ++ ++ +N S        SS 
Sbjct: 841 ASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSK 900

BLAST of Chy12G210650 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 793.9 bits (2049), Expect = 1.4e-229
Identity = 415/900 (46.11%), Postives = 564/900 (62.67%), Query Frame = 0

Query: 16  SLLILILFLFTSFLPLPATSLPAVGFGYRIRSSHVDPAGKSLTADLDLIGTSQVYGPDLP 75
           SLL+ I+  F+S          A+G GYR+ S    P   S    L +  ++++YG D+ 
Sbjct: 6   SLLVAIILCFSSL-----QCSNAIGKGYRLISMEKSPDDGSFIGYLQVKQSNKIYGSDIT 65

Query: 76  TLTLQATFESKDRLRVRITDSTRERWEIPHHIIPRRSNSLIRSLPENHVASPKAS----- 135
            L L   + +  RLRV ITD+ ++RWE+P+       N L R  P N +   + S     
Sbjct: 66  ILRLFINYRTDHRLRVHITDAKKQRWEVPY-------NLLRREQPPNVIGKSRKSPVTVQ 125

Query: 136 FISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDSETF--LVFKDQYIQLSSS 195
            IS P   LIFT+    PF F+V RRS+G+ +F+TS   S  E+F  +VFKDQY+++S+S
Sbjct: 126 EISGPELILIFTVD---PFSFAVRRRSNGETIFNTS---SSDESFGEMVFKDQYLEISTS 185

Query: 196 LPKDRSSIFGIGEQTR-KSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPS 255
           LPKD +S++G GE ++    KLVP  N+  TL+  D+ + NL+ +LYG+HP Y+DLR+ S
Sbjct: 186 LPKD-ASLYGFGENSQANGIKLVP--NEPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNVS 245

Query: 256 QDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELI 315
             GK  A   H VLLLNS+GMD+ Y GD +TYKVIGG+ D YFFAGPSP++V+DQYT LI
Sbjct: 246 --GKAYA---HSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSPLNVVDQYTSLI 305

Query: 316 GRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDP 375
           GRPAP+PYWS GFHQCR+GY NVS ++ VV  Y KA IPL+ +W D DYMDGYKDFT D 
Sbjct: 306 GRPAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKDFTLDL 365

Query: 376 INFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFMKYNGVPYLGDV 435
           +NFP  K+  F+D +HK G KYV+I DPGI  N +YG Y RG  +D+F+KY G P+L  V
Sbjct: 366 VNFPHAKLLSFLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFIKYEGKPFLAQV 425

Query: 436 WPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTNPLSNLDNPP 495
           WPGPVYFPDF +P + ++WG EI+ F ++VP DGLWIDMNE                   
Sbjct: 426 WPGPVYFPDFLNPKTVSWWGDEIRRFHELVPIDGLWIDMNE------------------- 485

Query: 496 YMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASLVKVTGQRPFVLS 555
             IN    +  L  KT+P S  H+  + EY+ H++YGF E+ ATH +L+ V G+RPF+LS
Sbjct: 486 --INATGHKASLGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALLAVQGKRPFILS 545

Query: 556 RSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCR 615
           RSTFVGSG+Y AHWTGD   TW  L  +I ++LNFG+FG+PMVGSDICGF   T EELC 
Sbjct: 546 RSTFVGSGQYAAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCN 605

Query: 616 RWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKG 675
           RWI++GAFYPF+RDH+D  + R+ELY W +VA SAR  L +RY+LLP+ YTL YEAH  G
Sbjct: 606 RWIEVGAFYPFSRDHADYYAPRKELYQWGTVAESARNALGMRYKLLPFLYTLNYEAHMSG 665

Query: 676 TPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVLKEGAFSVEAYFPAGNWFSLFNYSE 735
            PIARPLFFSFP+  + Y +  QFLLG  +++SPVL++G   VEA FP G+W+ +F+ ++
Sbjct: 666 APIARPLFFSFPEFTECYGLSKQFLLGSSLMISPVLEQGKTQVEALFPPGSWYHMFDMTQ 725

Query: 736 FVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFG 795
            V   +G+   L AP + +NVH+ +  IL +         A E           G +S G
Sbjct: 726 VVVSKNGRLFTLPAPFNVVNVHLYQNAILPMQQVVAFPAGASE-----------GYAS-G 785

Query: 796 EVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGF 855
           ++FLDD E+ EM    G  + + FY+      + + SQV  G FALSQ L+I+KV  +G 
Sbjct: 786 KLFLDDDELPEMKLGNGKSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKVIVLGL 845

Query: 856 ERPKKMVDLGLNISKGLNLNGNSSIRKTYQYF---------AKFMNAEISGLSIPIWEEF 899
           +   K+ ++ LN S   N      +    Q +         +K    E+ GL + + ++F
Sbjct: 846 KGTWKVSEILLNGSSISNETKTIEVSSKEQMYVVGSEDEGESKSFMVELKGLEMLVGKDF 846

BLAST of Chy12G210650 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 323.9 bits (829), Expect = 4.0e-88
Identity = 221/689 (32.08%), Postives = 331/689 (48.04%), Query Frame = 0

Query: 192 DRSSIFGIGEQTRKSFKLVPDK------NKTLTLWNADIGSVNLD--VNLYGAHPFYIDL 251
           D S ++GI E    SF L P K      ++   L+N D+   + +    LYG+ PF +  
Sbjct: 222 DSSFVYGIPEHA-TSFALKPTKGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMV-- 281

Query: 252 RSPSQDGKVAAGTTHGVLLLNS---------------NGMDIIYSGDRIT--YKVIGGII 311
                 GK  +G T G   LN+               +G+ +  S  RI   +    GI+
Sbjct: 282 ----SHGK--SGKTSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIV 341

Query: 312 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIP 371
           D +FF GP P  V+ QY  + G  A    ++ G+HQCR+ Y +  D+  V +++ +  IP
Sbjct: 342 DTFFFVGPEPKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIP 401

Query: 372 LEAMWTDIDYMDGYKDFTFDPINFPSDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY 431
            + +W DI++ DG + FT+D + FP  +       L   G+K V I+DP I  +++Y  +
Sbjct: 402 YDVLWLDIEHTDGKRYFTWDSVLFPHPEE--MQKKLAAKGRKMVTIVDPHIKRDDSYFLH 461

Query: 432 IRGTEADIFMK-YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQI--FRDIVPFDGLW 491
              T+   ++K  +G  + G  WPG   + D   P+   +WG       +    P    W
Sbjct: 462 KEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYTW 521

Query: 492 IDMNEISNFITSSTNPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNMAEYNTHNLY 551
            DMNE                  P + N   V       T+P   LH G +     HN Y
Sbjct: 522 NDMNE------------------PSVFNGPEV-------TMPRDALHVGGVEHREVHNAY 581

Query: 552 GFLESRATHASLV--KVTGQRPFVLSRSTFVGSGKYTAHWTGDIGATWNDLGYTIPSILN 611
           G+    AT   LV  +    RPFVLSR+ F G+ +Y A WTGD  A W  L  +IP IL 
Sbjct: 582 GYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMILT 641

Query: 612 FGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLW-DSVAA 671
            GL GI   G+DI GF G+   EL  RW Q+GA+YPF R H+   + R+E +L+ +    
Sbjct: 642 LGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNTE 701

Query: 672 SARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVS 731
             R  +  RY LLPYFYTL  EA+  G P+ RPL+  FPQD  T+  D  F++G G+LV 
Sbjct: 702 LMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSGLLVQ 761

Query: 732 PVLKEGAFSVEAYFPA-GNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALH 791
            V  +G      Y P   +W+ L N   +V    G+   +DAP + I    + G I+   
Sbjct: 762 GVYTKGTTQASVYLPGKESWYDLRNGKTYV---GGKTHKMDAPEESIPAFQKAGTIIPRK 821

Query: 792 GEAMTTRAAQET-PYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGS 847
                + +  +  PY L+V +++ Q + GE+++DDG+  E  R  G++   RF    V S
Sbjct: 822 DRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRR--GSYIHRRF----VFS 865

BLAST of Chy12G210650 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 300.1 bits (767), Expect = 6.2e-81
Identity = 195/644 (30.28%), Postives = 318/644 (49.38%), Query Frame = 0

Query: 194 SSIFGIGEQTRKSFKLVPDKNKTLTLWNADI-GSVNLDVNLYGAHPFYIDLRSPSQDGKV 253
           +S +G GE + +    +    K +  WN D  G  +   +LY +HP+ + +    +   V
Sbjct: 86  TSFYGTGEVSGQ----LERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGV 145

Query: 254 AAGTTHGVLL-LNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 313
            A TT    + L   G+  I S    +Y +I       F    SP +V++  +  IG   
Sbjct: 146 LADTTRKCEIDLRKEGIIRIIS--PASYPII------TFGPFSSPTAVLESLSHAIGTVF 205

Query: 314 PVPYWSFGFHQCRYGYNNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFP 373
             P W+ G+HQCR+ Y +   +  +   +    IP + +W DIDYMDG++ FTFD   FP
Sbjct: 206 MPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFP 265

Query: 374 SDKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTEADIFM-KYNGVPYLGDVWPG 433
                    +LH NG K + +LDPGI     Y  Y  G++ D+++ + +G P+ G+VWPG
Sbjct: 266 DP--SALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPG 325

Query: 434 PVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNF-ITSSTNPLSNLDNPPYM 493
           P  FPD+ +  + ++W   ++ F      DG+W DMNE + F + + T P +N+ +    
Sbjct: 326 PCVFPDYTNSKARSWWANLVKEFVS-NGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDDE 385

Query: 494 INNARVQRPLNNKTVPASILHFGNMAEYNTHNLYGFLESRATHASL-VKVTGQRPFVLSR 553
           +   +                  N + Y  HN+YG L +R+T+  + +    +RPFVL+R
Sbjct: 386 LGGVQ------------------NHSHY--HNVYGMLMARSTYEGMELADKNKRPFVLTR 445

Query: 554 STFVGSGKYTAHWTGDIGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 613
           + F+GS +Y A WTGD  + W  L  +I  +L  GL G P+ G DI GF+G+ T  L  R
Sbjct: 446 AGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGR 505

Query: 614 WIQLGAFYPFARDHSDKGSIRQELYLW-DSVAASARKVLALRYQLLPYFYTLMYEAHKKG 673
           W+ +GA +PF R HS+ G+   E + + +      R  L  RYQLLP+FYTL Y AH  G
Sbjct: 506 WMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTG 565

Query: 674 TPIARPLFFSFPQDIKTYEIDSQFLLGGGVLVSPVL-KEGAFSVEAYFPAGNWFSLFNYS 733
            P+A P+FF+ P D +   +++ FLLG  ++ +  L  +G+  ++   P G W   F+++
Sbjct: 566 APVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHR-FDFA 625

Query: 734 EFVAVNSGQQINLDAPADHINVHVREGNILALHGEAM-TTRAAQETPYKLLVVISNGQSS 793
                        D+  D   ++++ G+I++L    +     +      LLV +     +
Sbjct: 626 -------------DSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKA 678

Query: 794 FGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVING 830
            G +F DDG+    G   G + +  + +E   S + VK     G
Sbjct: 686 KGLLFEDDGD--GYGYTKGRFLVTHYIAERDSSTVTVKVSKTEG 678

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048930.0e+0059.07Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1[more]
O049314.8e-30456.44Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1[more]
Q653V73.7e-28854.97Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q437632.7e-27555.32Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1[more]
Q9S7Y71.2e-23344.74Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M3Y30.0e+0097.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G716250 PE=3 SV=1[more]
A0A5D3E1Q30.0e+0094.28Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00... [more]
A0A1S3C8V00.0e+0094.28alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1[more]
A0A6J1KFK10.0e+0085.11alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1[more]
A0A6J1ENT60.0e+0084.44alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011660330.10.097.46alpha-glucosidase [Cucumis sativus] >KGN66921.1 hypothetical protein Csa_007658 ... [more]
XP_008459069.10.094.28PREDICTED: alpha-glucosidase [Cucumis melo] >KAA0043261.1 alpha-glucosidase [Cuc... [more]
XP_038894574.10.088.06alpha-glucosidase [Benincasa hispida][more]
XP_023520110.10.085.38alpha-glucosidase [Cucurbita pepo subsp. pepo][more]
XP_023001112.10.085.38alpha-glucosidase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G11720.10.0e+0062.43Glycosyl hydrolases family 31 protein [more]
AT1G68560.18.3e-23544.74alpha-xylosidase 1 [more]
AT3G45940.11.4e-22946.11Glycosyl hydrolases family 31 protein [more]
AT5G63840.14.0e-8832.08Glycosyl hydrolases family 31 protein [more]
AT3G23640.16.2e-8130.28heteroglycan glucosidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000322Glycoside hydrolase family 31PFAMPF01055Glyco_hydro_31coord: 289..756
e-value: 1.2E-156
score: 522.3
IPR031727Galactose mutarotase, N-terminal barrelPFAMPF16863NtCtMGAM_Ncoord: 57..190
e-value: 9.1E-33
score: 112.8
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 652..752
e-value: 5.3E-35
score: 121.5
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 753..904
e-value: 1.1E-29
score: 105.5
NoneNo IPR availableGENE3D2.60.40.1760glycosyl hydrolase (family 31)coord: 37..309
e-value: 2.7E-94
score: 317.8
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 310..651
e-value: 1.5E-155
score: 519.6
NoneNo IPR availablePANTHERPTHR22762ALPHA-GLUCOSIDASEcoord: 33..902
NoneNo IPR availablePANTHERPTHR22762:SF150ALPHA-GLUCOSIDASE-LIKEcoord: 33..902
NoneNo IPR availableCDDcd14752GH31_Ncoord: 183..308
e-value: 7.65443E-24
score: 95.3304
NoneNo IPR availableCDDcd06602GH31_MGAM_SI_GAAcoord: 308..664
e-value: 0.0
score: 626.075
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 669..752
IPR025887Glycoside hydrolase family 31, N-terminal domainPFAMPF13802Gal_mutarotas_2coord: 195..268
e-value: 1.8E-6
score: 28.2
IPR030458Glycosyl hydrolases family 31, active sitePROSITEPS00129GLYCOSYL_HYDROL_F31_1coord: 461..468
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 299..667
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 43..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G210650.1Chy12G210650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0090599 alpha-glucosidase activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds