Chy12G210380 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G210380
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	twinkle homolog protein, chloroplastic/mitochondrial isoform X1
Location	chrH12: 5319650 .. 5321145 (-)
RNA-Seq Expression	Chy12G210380
Synteny	Chy12G210380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCTTGTGAAACCCTTTGCCATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAAACCTCCAGGTGCTATTATTGGAACTTTTTATGGTTGTTGAATTGTAGTTGGGTTTGTGATTGTTTCTGGTTAATACCCATGTTGTGGGGTAACTTGATGTTTCTTGGCTGGGTGGTTACTTTTTTTTTTCCCACTTTTTGGATTGGGATGAAACAGGAGAGGTTAAGGGTATATTTTTGAAGGCAGCAAAGTAGTTTGCAGGCAATGTATTCTGAGGATGGGATTTGGGAGAGGACTTGACGTTTGTTTAGTTTTAATGTTATTCGCTTTCTTCTTTGTGCAGCCCTTTTGGAGAATCCACTAGACAAGGCTTCAAGCTCCACTCGATTGAATATTCTGAGAAAAAAATTGCAGGATCTTGACATTGACATCGAAGCTTGTGTTCCTGGGCAGTTTTACAGTTTGCTTTGTCCAATGGTATTTTGTTTCTAATATCAGTTTCTTTATGCACATTGTGTTCTGTTTTGAGATATTCATCCAATTTGTAGAAGTGAATGAAAAAAGCTGCCAACTTCTATATCTTATTTATTTTTGTATTTATTTTTGGTAAGAAACTAGAAGATATATGGATGAAAAAAAGAAGACAGCCTAAGGGCAGGAAAGAGACCCCCTTCCAAAGGTGTTAATAAAATTAATAAAAGCAGCAAGCATATCTTCAGGAGTCCAATGACTTGTTGCGGTCAAAATTCATCAATTCTAATCAGTTTTCAATTGAAATTCATGAATTGAGGACTAATCTTGGTCTAAGTTCAAGAATTTGGACCACATCTCAGTTGGAATTAGTGAGTTTCAAAGAGTTTTCGGCAGAACTCATGAATTCAGATTTCGACTTCTGCTTCTTTGAAAAGATGTATAAGAAAATCTATTTTACAATTCCTCATTCAATTTGGAGAGGGAAAGTGGGTTAAAAAGTTGATGAATATGGAATGAAAGCAAAAAATGGAAAGAAAGGAAAAGATTTGCATTGGAATCTAGAAAGCAAAAGATGTGCATCAAAAAGAGTCTTTATCAACTTAAATTTGCTCCAAGGGACTCTGCTTCATTAGTTTTTGTTTGGGGCAATCGGATCTAGTTCTGATGTAGTTAGCTTGGCTGTTGGTTATTAGTTGATGTAATTAGCTTGGTTATTAACTGCTGGAAATTACTCACACTTTTAGATTTTTGACCTGTTGTAAAGGTGGTAGGTCACCTTCTGCCTTAGTATAGAACTTGGAATTAGGCTGTTCTAATTTTTTTTGAATTCAGATTTTAATAAGAAGAATAAAGACTTCAGTTTTTTTGTTTGAACTTCTCCAAGACTTCATCAACTTGGTATCGGAGCTCTCGAAGAAGGGACTCATGGTGGGAAGGAACCCAGCCAACATTGGCAAAGGCAAGCGTGTCAATGAGCAACCCTTGGAACCAAAGAGATTCTCTCACTGA

mRNA sequence

ATGTCCCTTGTGAAACCCTTTGCCATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAAACCTCCAGCCCTTTTGGAGAATCCACTAGACAAGGCTTCAAGCTCCACTCGATTGAATATTCTGAGAAAAAAATTGCAGGATCTTGACATTGACATCGAAGCTTGTGTTCCTGGGCAGTTTTACAGTTTGCTTTGTCCAATGATTTTAATAAGAAGAATAAAGACTTCAGTTTTTTTGTTTGAACTTCTCCAAGACTTCATCAACTTGGTATCGGAGCTCTCGAAGAAGGGACTCATGGTGGGAAGGAACCCAGCCAACATTGGCAAAGGCAAGCGTGTCAATGAGCAACCCTTGGAACCAAAGAGATTCTCTCACTGA

Coding sequence (CDS)

ATGTCCCTTGTGAAACCCTTTGCCATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAAACCTCCAGCCCTTTTGGAGAATCCACTAGACAAGGCTTCAAGCTCCACTCGATTGAATATTCTGAGAAAAAAATTGCAGGATCTTGACATTGACATCGAAGCTTGTGTTCCTGGGCAGTTTTACAGTTTGCTTTGTCCAATGATTTTAATAAGAAGAATAAAGACTTCAGTTTTTTTGTTTGAACTTCTCCAAGACTTCATCAACTTGGTATCGGAGCTCTCGAAGAAGGGACTCATGGTGGGAAGGAACCCAGCCAACATTGGCAAAGGCAAGCGTGTCAATGAGCAACCCTTGGAACCAAAGAGATTCTCTCACTGA

Protein sequence

MSLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACVPGQFYSLLCPMILIRRIKTSVFLFELLQDFINLVSELSKKGLMVGRNPANIGKGKRVNEQPLEPKRFSH*

Homology

[Close]

BLAST of Chy12G210380 vs. ExPASy Swiss-Prot
Match: B5X582 (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.1e-05
Identity = 27/66 (40.91%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 6   PFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACVPGQF 65
           P+  + NG+SS+ S   VP  P   E   DK    +RL  LR+KL +  +D E C PGQ 
Sbjct: 73  PYYQRTNGLSSYNSIPRVP-TPVDTEVEADKRVVLSRLVTLRRKLAEQGVDAENCPPGQH 132

Query: 66  YSLLCP 72
             L+CP
Sbjct: 133 SGLICP 137

[Close]

BLAST of Chy12G210380 vs. ExPASy TrEMBL
Match: A0A5A7TYL9 (Twinkle-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00560 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.1e-46
Identity = 100/116 (86.21%), Postives = 102/116 (87.93%), Query Frame = 0


Query: 9   MKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACVPGQFYSL 68
           MKPNG SSFTSHANVP+PPA  ENP DKA SST LNILRKKLQDL IDIEACVPGQF  L
Sbjct: 1   MKPNGFSSFTSHANVPRPPAFSENPRDKALSSTLLNILRKKLQDLGIDIEACVPGQFSGL 60

Query: 69  LCPMILIRRIKTSVFLFELLQDFINLVSELSKKGLMVGRNPANIGKGKRVNEQPLE 125
           LCPMILIRRIKTSV LFELLQDFINLVSEL KKGLMVGRNPAN GKGKR +EQP E
Sbjct: 61  LCPMILIRRIKTSVLLFELLQDFINLVSELLKKGLMVGRNPANAGKGKRFDEQPFE 116

[Close]

BLAST of Chy12G210380 vs. ExPASy TrEMBL
Match: A0A0A0LYM8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G541890 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.6e-30
Identity = 69/72 (95.83%), Postives = 71/72 (98.61%), Query Frame = 0


Query: 1   MSLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEAC 60
           +SLVKPFAMKPNGVSSFTSHANVP+PPALLENP DKASSSTRLNILRKKLQDLDIDIEAC
Sbjct: 73  LSLVKPFAMKPNGVSSFTSHANVPRPPALLENPPDKASSSTRLNILRKKLQDLDIDIEAC 132

Query: 61  VPGQFYSLLCPM 73
           VPGQFYSLLCPM
Sbjct: 133 VPGQFYSLLCPM 144

[Close]

BLAST of Chy12G210380 vs. ExPASy TrEMBL
Match: A0A1S3BDI3 (twinkle homolog protein, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488704 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.3e-24
Identity = 64/89 (71.91%), Postives = 67/89 (75.28%), Query Frame = 0


Query: 2   SLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACV 61
           SLVKPFAMKPNG SSFTSHANVP+PPA  ENP DKA SST LNILRKKLQDL IDIEACV
Sbjct: 77  SLVKPFAMKPNGFSSFTSHANVPRPPAFSENPRDKALSSTLLNILRKKLQDLGIDIEACV 136

Query: 62  PGQFYSLLCPMILIRRIKTSVFLFELLQD 91
           PGQF  LLCPM      K   F   + +D
Sbjct: 137 PGQFSGLLCPMCKGGDSKERSFALHISED 165

[Close]

BLAST of Chy12G210380 vs. ExPASy TrEMBL
Match: A0A1S3BD14 (twinkle homolog protein, chloroplastic/mitochondrial isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488704 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.3e-24
Identity = 64/89 (71.91%), Postives = 67/89 (75.28%), Query Frame = 0


Query: 2   SLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACV 61
           SLVKPFAMKPNG SSFTSHANVP+PPA  ENP DKA SST LNILRKKLQDL IDIEACV
Sbjct: 77  SLVKPFAMKPNGFSSFTSHANVPRPPAFSENPRDKALSSTLLNILRKKLQDLGIDIEACV 136

Query: 62  PGQFYSLLCPMILIRRIKTSVFLFELLQD 91
           PGQF  LLCPM      K   F   + +D
Sbjct: 137 PGQFSGLLCPMCKGGDSKERSFALHISED 165

[Close]

BLAST of Chy12G210380 vs. ExPASy TrEMBL
Match: A0A0A0LVL8 (Toprim domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542410 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.5e-23
Identity = 62/82 (75.61%), Postives = 65/82 (79.27%), Query Frame = 0


Query: 9  MKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACVPGQFYSL 68
          MKPNGVSSFTSHANVP+PPALL NP DKAS STRLNILRKKLQDLDIDIEACVPGQF SL
Sbjct: 1  MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60

Query: 69 LCPMILIRRIKTSVFLFELLQD 91
          LCPM      K   F   + +D
Sbjct: 61 LCPMCKGGDTKERSFSLNISED 82

[Close]

BLAST of Chy12G210380 vs. NCBI nr
Match: KAA0047076.1 (twinkle-like protein [Cucumis melo var. makuwa] >TYK05081.1 twinkle-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 195 bits (496), Expect = 1.26e-60
Identity = 100/116 (86.21%), Postives = 102/116 (87.93%), Query Frame = 0


Query: 9   MKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACVPGQFYSL 68
           MKPNG SSFTSHANVP+PPA  ENP DKA SST LNILRKKLQDL IDIEACVPGQF  L
Sbjct: 1   MKPNGFSSFTSHANVPRPPAFSENPRDKALSSTLLNILRKKLQDLGIDIEACVPGQFSGL 60

Query: 69  LCPMILIRRIKTSVFLFELLQDFINLVSELSKKGLMVGRNPANIGKGKRVNEQPLE 124
           LCPMILIRRIKTSV LFELLQDFINLVSEL KKGLMVGRNPAN GKGKR +EQP E
Sbjct: 61  LCPMILIRRIKTSVLLFELLQDFINLVSELLKKGLMVGRNPANAGKGKRFDEQPFE 116

[Close]

BLAST of Chy12G210380 vs. NCBI nr
Match: XP_011659233.2 (twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus])

HSP 1 Score: 143 bits (360), Expect = 1.16e-36
Identity = 70/72 (97.22%), Postives = 71/72 (98.61%), Query Frame = 0


Query: 1   MSLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEAC 60
           MSLVKPFAMKPNGVSSFTSHANVP+PPALLENP DKASSSTRLNILRKKLQDLDIDIEAC
Sbjct: 73  MSLVKPFAMKPNGVSSFTSHANVPRPPALLENPPDKASSSTRLNILRKKLQDLDIDIEAC 132

Query: 61  VPGQFYSLLCPM 72
           VPGQFYSLLCPM
Sbjct: 133 VPGQFYSLLCPM 144

[Close]

BLAST of Chy12G210380 vs. NCBI nr
Match: XP_011659240.2 (twinkle homolog protein, chloroplastic/mitochondrial isoform X5 [Cucumis sativus])

HSP 1 Score: 133 bits (335), Expect = 1.88e-33
Identity = 66/72 (91.67%), Postives = 68/72 (94.44%), Query Frame = 0


Query: 1   MSLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEAC 60
           +SLVKPFAMKPNGVSSFTSHANVP+PPALL NP DKAS STRLNILRKKLQDLDIDIEAC
Sbjct: 70  LSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEAC 129

Query: 61  VPGQFYSLLCPM 72
           VPGQF SLLCPM
Sbjct: 130 VPGQFSSLLCPM 141

[Close]

BLAST of Chy12G210380 vs. NCBI nr
Match: XP_031736083.1 (twinkle homolog protein, chloroplastic/mitochondrial isoform X4 [Cucumis sativus])

HSP 1 Score: 133 bits (335), Expect = 2.30e-33
Identity = 66/72 (91.67%), Postives = 68/72 (94.44%), Query Frame = 0


Query: 1   MSLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEAC 60
           +SLVKPFAMKPNGVSSFTSHANVP+PPALL NP DKAS STRLNILRKKLQDLDIDIEAC
Sbjct: 70  LSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEAC 129

Query: 61  VPGQFYSLLCPM 72
           VPGQF SLLCPM
Sbjct: 130 VPGQFSSLLCPM 141

[Close]

BLAST of Chy12G210380 vs. NCBI nr
Match: XP_031736081.1 (twinkle homolog protein, chloroplastic/mitochondrial isoform X3 [Cucumis sativus] >XP_031736082.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X3 [Cucumis sativus])

HSP 1 Score: 133 bits (335), Expect = 2.41e-33
Identity = 66/72 (91.67%), Postives = 68/72 (94.44%), Query Frame = 0


Query: 1   MSLVKPFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEAC 60
           +SLVKPFAMKPNGVSSFTSHANVP+PPALL NP DKAS STRLNILRKKLQDLDIDIEAC
Sbjct: 70  LSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEAC 129

Query: 61  VPGQFYSLLCPM 72
           VPGQF SLLCPM
Sbjct: 130 VPGQFSSLLCPM 141

[Close]

BLAST of Chy12G210380 vs. TAIR 10
Match: AT1G30680.1 (toprim domain-containing protein )

HSP 1 Score: 48.9 bits (115), Expect = 3.6e-06
Identity = 27/66 (40.91%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 6   PFAMKPNGVSSFTSHANVPKPPALLENPLDKASSSTRLNILRKKLQDLDIDIEACVPGQF 65
           P+  + NG+SS+ S   VP  P   E   DK    +RL  LR+KL +  +D E C PGQ 
Sbjct: 73  PYYQRTNGLSSYNSIPRVP-TPVDTEVEADKRVVLSRLVTLRRKLAEQGVDAENCPPGQH 132

Query: 66  YSLLCP 72
             L+CP
Sbjct: 133 SGLICP 137

The following BLAST results are available for this feature:

BLAST of Chy12G210380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5X582	5.1e-05	40.91	Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=...	[more]

BLAST of Chy12G210380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TYL9	2.1e-46	86.21	Twinkle-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold131...	[more]
A0A0A0LYM8	1.6e-30	95.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G541890 PE=4 SV=1	[more]
A0A1S3BDI3	1.3e-24	71.91	twinkle homolog protein, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo ...	[more]
A0A1S3BD14	1.3e-24	71.91	twinkle homolog protein, chloroplastic/mitochondrial isoform X2 OS=Cucumis melo ...	[more]
A0A0A0LVL8	1.5e-23	75.61	Toprim domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542410 PE=4...	[more]

BLAST of Chy12G210380 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0047076.1	1.26e-60	86.21	twinkle-like protein [Cucumis melo var. makuwa] >TYK05081.1 twinkle-like protein...	[more]
XP_011659233.2	1.16e-36	97.22	twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus]	[more]
XP_011659240.2	1.88e-33	91.67	twinkle homolog protein, chloroplastic/mitochondrial isoform X5 [Cucumis sativus...	[more]
XP_031736083.1	2.30e-33	91.67	twinkle homolog protein, chloroplastic/mitochondrial isoform X4 [Cucumis sativus...	[more]
XP_031736081.1	2.41e-33	91.67	twinkle homolog protein, chloroplastic/mitochondrial isoform X3 [Cucumis sativus...	[more]

BLAST of Chy12G210380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30680.1	3.6e-06	40.91	toprim domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 109..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G210380.1	Chy12G210380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006260	DNA replication
molecular_function	GO:0043139	5'-3' DNA helicase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003697	single-stranded DNA binding