Homology
BLAST of Chy12G210320 vs. ExPASy Swiss-Prot
Match:
Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)
HSP 1 Score: 526.2 bits (1354), Expect = 4.4e-148
Identity = 262/537 (48.79%), Postives = 365/537 (67.97%), Query Frame = 0
Query: 4 SSPLTPLTVAFILLS-PYPLWVVASSNNYKHQAFLQCLS-------SITRVIYTPINFSY 63
SS L T + +LLS P+P+ S+N +FLQCLS +++VI+TP + S+
Sbjct: 8 SSVLIFFTTSTLLLSIPHPV----SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 67
Query: 64 FSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGL 123
SVL SIQN RFS P+ PKP+ I+TP S +Q A+ C+R G + IRTRSGGHD+EGL
Sbjct: 68 SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFG-IHIRTRSGGHDYEGL 127
Query: 124 SYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSV 183
SYV + PF+ILDL NLRSI++DV N + WVQ+GAT+GELYY+I +K+RTLAFPAGVCP+V
Sbjct: 128 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTV 187
Query: 184 GIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFG 243
G+GG SGGGYG LLRK+GLA D+VIDA +VDA G + +R MGED FWAIRGGGG SF
Sbjct: 188 GVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFC 247
Query: 244 IVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNISS 303
+V++WK+ L+++P+TVT+ + + A+++I+ WQ+VADK+ ++L + ++L
Sbjct: 248 VVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML------- 307
Query: 304 EGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTL--IEINGSPT 363
+ K ASF L+LG LL ++NK FP+LG+ + DCT+ SWIES + E+ P
Sbjct: 308 QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPI 367
Query: 364 NNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKM 423
N +L +++++ +FK KSD+VQ+P+P AI +W RL+ + + L P+GGKM
Sbjct: 368 N-----VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKM 427
Query: 424 CKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAY 483
+I D ETPFPHR GN+Y I YL W +++E+++ W+ +Y+ M+ FV+K PR AY
Sbjct: 428 SEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAY 487
Query: 484 VNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIP 531
+N RDLD+G ++ +E+ WG KYF NF RLV VKT VDP D F EQSIP
Sbjct: 488 INLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524
BLAST of Chy12G210320 vs. ExPASy Swiss-Prot
Match:
Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)
HSP 1 Score: 524.6 bits (1350), Expect = 1.3e-147
Identity = 262/544 (48.16%), Postives = 368/544 (67.65%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLS-------SITRVIYTPINF 60
M+ SS L + + + +SP + S++ H+ FL+CLS +VI+T +
Sbjct: 1 MEFSSFLFTILLFSLNISP-----LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDS 60
Query: 61 SYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 120
S+FS+LD SIQN RFS ETPKP++IITP S +Q I C++ HG + +RTRS GH +E
Sbjct: 61 SFFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHG-IHVRTRSAGHCYE 120
Query: 121 GLSYVAYH-PFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVC 180
GLSY+AY+ PF ++DL NLRSIS+DV N T WVQ+GAT GELYY+I + +++LAFPAG+
Sbjct: 121 GLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIH 180
Query: 181 PSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGG 240
P+VG+GG SGGGYG LLRKYGLA DN+IDA +VDA+G + DR++MGED FWAIRGGGG
Sbjct: 181 PTVGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGS 240
Query: 241 SFGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGN 300
SFG++++WK++LV +P+T+T+ +T K A+ +I +WQY ADK+ ++L + L N
Sbjct: 241 SFGVILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN 300
Query: 301 ISSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSP 360
K A F L++G N LL+++ + FP+LG+ K+ C + SWIES L P
Sbjct: 301 ------KNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVL-WFADFP 360
Query: 361 TNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGK 420
SL L NR+ S+ SFK K D+VQ+PIP AI+ IW RL++ + + + + P+GGK
Sbjct: 361 KGESLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGK 420
Query: 421 MCKIFDLETPFPHRAGNLYMIGYLVGW---ENQSKEIEERHLSWIREIYNYMTPFVSKFP 480
M ++ + ETPFPHR GNLY I Y+ W E+++K +++L W+ +Y +MTP+VSK P
Sbjct: 421 MSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSP 480
Query: 481 RAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSI 534
R AYVN++D+D+G KT +E+ WG KYF NF RLV VKT+VDP D F EQSI
Sbjct: 481 RGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSI 530
BLAST of Chy12G210320 vs. ExPASy Swiss-Prot
Match:
Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)
HSP 1 Score: 511.9 bits (1317), Expect = 8.6e-144
Identity = 263/540 (48.70%), Postives = 361/540 (66.85%), Query Frame = 0
Query: 7 LTPLTVAFIL----LSPYPLWVVASSNNYKHQAFLQCLS-----SITRVIYTPINFSYFS 66
L L + F+L LS +P S+ H+ FL+CLS +R+I+T + SYFS
Sbjct: 7 LLSLFIYFLLLNLSLSHFP---SISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFS 66
Query: 67 VLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSY 126
+L+ SIQN RF ETPKP++IITP + +Q I C+R HG + IRTRSGGHD+EGLSY
Sbjct: 67 ILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHG-IHIRTRSGGHDYEGLSY 126
Query: 127 VA-YHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVG 186
+A PF+++DL NLRSI++DV N T WVQSGAT+GELYY+I + S++LAFPAG+ P+VG
Sbjct: 127 MAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVG 186
Query: 187 IGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGI 246
IGG GGGYG L+RKYGL+ DNVIDA++VDANG DR+ MGED FWAIRGGGG SF +
Sbjct: 187 IGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSV 246
Query: 247 VVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNISSE 306
V++WK+RL+ +P+ VT+ +T + A+ +I +WQY+ADK+ +L + +L
Sbjct: 247 VLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ------- 306
Query: 307 GGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSPTNNS 366
+ ASF L+LG + LL+++ FP+LG+ +C + SWIES L I G S
Sbjct: 307 -KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKG----ES 366
Query: 367 LQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKI 426
++ L RK S SFK K D++++PIP AI+ +W R ++ + + + + P+GGKM +I
Sbjct: 367 MEILAKRKRTS-RSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 426
Query: 427 FDLETPFPHRAGNLYMIGYLVGW---ENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAY 486
D E PFPHR GNLY I YL W E+++K E++L W+ +Y +MTP+VSK PR AY
Sbjct: 427 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 486
Query: 487 VNYRDLDIGTNTEYG-KTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTL 533
VN+RD+D+G KT +E+A +WG KYF NF+RLV VKT VDP D F EQSIP +
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529
BLAST of Chy12G210320 vs. ExPASy Swiss-Prot
Match:
Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)
HSP 1 Score: 506.9 bits (1304), Expect = 2.8e-142
Identity = 260/543 (47.88%), Postives = 363/543 (66.85%), Query Frame = 0
Query: 4 SSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSSITRVIYTPINFSYFS------ 63
S PL ++ + S Y + +S + + Q F+ C+ T V Y P+ ++F+
Sbjct: 4 SKPLPLFSILVLYFSLYTITPTSSLASLQDQ-FINCVQRNTHV-YFPLEKTFFAPTKNVS 63
Query: 64 ----VLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 123
VL+ + QNLRF K PKP I +P H S +Q +IICS+ + +R RSGGHD+E
Sbjct: 64 MFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKL-RMHLRVRSGGHDYE 123
Query: 124 GLSYVAY--HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGV 183
GLSYV+ PFI++DL +R ++I++++N+AWVQSGATVGELYY+IAEKS+ FPAG+
Sbjct: 124 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 183
Query: 184 CPSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGG 243
C S+GIGG I+GG YG ++RKYGL DNV+DA +VDANG++ DR +MGED FWAIRGG G
Sbjct: 184 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 243
Query: 244 GSFGIVVAWKLRLVSIPATVTICISNRT-QKDGAIELIYEWQYVADKLDENLHLGILLNG 303
GSFGI++AWK++LV +P TVT+ +T Q+D ++I +WQ VADKL E L + +L
Sbjct: 244 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF-- 303
Query: 304 GNISSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEING 363
N++ GG T S+ LFLG L++++ K+FP+LG+T KDC + SW+ES + I+G
Sbjct: 304 -NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLES-IAYISG 363
Query: 364 SPTNNSLQTLLNRKSQ-SIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPY 423
PT+ LL KS SFK KSD+V+ PIP ++GI+++L +D+ + PY
Sbjct: 364 FPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWN--PY 423
Query: 424 GGKMCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFP 483
GG M KI + + PFPHR G L+ + Y+ W + K RH++WIR++Y+YMTP+VS P
Sbjct: 424 GGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKR-PSRHINWIRDLYSYMTPYVSSNP 483
Query: 484 RAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSI 533
R AYVNYRDLD+G NT+ KT +QA +WG YF NFNRL+ +K KVDP + FRHEQSI
Sbjct: 484 REAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSI 536
BLAST of Chy12G210320 vs. ExPASy Swiss-Prot
Match:
A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)
HSP 1 Score: 506.5 bits (1303), Expect = 3.6e-142
Identity = 258/510 (50.59%), Postives = 346/510 (67.84%), Query Frame = 0
Query: 36 FLQCLSSI-------TRVIYTPINFSYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQI 95
FL+C S +++YT N Y SVL+ +I NLRF+ TPKP+ I+TP+HVS I
Sbjct: 34 FLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVSHI 93
Query: 96 QVAIICSRTHGSLQIRTRSGGHDFEGLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSG 155
Q I+CS+ G LQIRTRSGGHD EG+SY++ PF+I+DL N+RSI IDV + TAWV++G
Sbjct: 94 QGTILCSKKVG-LQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEAG 153
Query: 156 ATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDA 215
AT+GE+YY + EK+ L+ AG CP+V GG GGGYG L+R YGLA DN+IDA+LV+
Sbjct: 154 ATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVNV 213
Query: 216 NGEVHDRESMGEDLFWAIRGGGGGSFGIVVAWKLRLVSIPATVTICISNRTQKDGAIELI 275
+G+V DR+SMGEDLFWA+RGGG SFGI+VAWK+RLV++P + + + ++L+
Sbjct: 214 HGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKIMEIHELVKLV 273
Query: 276 YEWQYVADKLDENLHLGILLNGGNISSEGGKPNPTA---SFLTLFLGKANKLLSILNKTF 335
+WQ +A K D++L L NI+ GK N TA F ++FLG + L+ ++NK+F
Sbjct: 274 NKWQNIAYKYDKDLLLMTHFITRNITDNQGK-NKTAIHTYFSSVFLGGVDSLVDLMNKSF 333
Query: 336 PQLGVTKKDCTQTSWIESTLI---EINGSPTNNSLQTLLNRKSQSIGSFKIKSDYVQQPI 395
P+LG+ K DC Q SWI++ + +N N + + LL+R + G+FKIK DYV++PI
Sbjct: 334 PELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPI 393
Query: 396 PLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKIFDLETPFPHRAGNLYMIGYLVGWENQ 455
P I E+L +D+ A + PYGG M +I + PFPHRAG LY + Y+ WE Q
Sbjct: 394 PESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQ 453
Query: 456 SKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKY 515
E E+HL+WIR IYN+MTP+VSK PR AY+NYRDLDIG N ++ QA IWG KY
Sbjct: 454 --EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEKY 513
Query: 516 FGKNFNRLVHVKTKVDPYDLFRHEQSIPTL 533
FGKNF+RLV VKT VDP + FR+EQSIP L
Sbjct: 514 FGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539
BLAST of Chy12G210320 vs. ExPASy TrEMBL
Match:
A0A1S3BDG0 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488675 PE=3 SV=1)
HSP 1 Score: 1026.5 bits (2653), Expect = 3.9e-296
Identity = 499/535 (93.27%), Postives = 519/535 (97.01%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSSITRVIYTPINFSYFSVLD 60
MK SSPL PLT++FILLSPYPLWVVASSNN K QAFLQCLSSI++VIY+PIN SYFSVLD
Sbjct: 1 MKLSSPLIPLTLSFILLSPYPLWVVASSNN-KPQAFLQCLSSISKVIYSPINSSYFSVLD 60
Query: 61 FSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAY 120
FSIQNLRFSKPETPKPIAIITP HVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVA+
Sbjct: 61 FSIQNLRFSKPETPKPIAIITPIHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAH 120
Query: 121 HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGF 180
HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSR+LAFPAGVCPSVGIGGF
Sbjct: 121 HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRSLAFPAGVCPSVGIGGF 180
Query: 181 ISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAW 240
+SGGGYGYLLRKYGLAVDNV+DAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAW
Sbjct: 181 VSGGGYGYLLRKYGLAVDNVLDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAW 240
Query: 241 KLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNISSEGGKP 300
KLRLV +P TVTICISN+T KDGAI+LIYEWQYVADKLDENL+LGILLNGGNISSEGGKP
Sbjct: 241 KLRLVLVPETVTICISNKTVKDGAIDLIYEWQYVADKLDENLYLGILLNGGNISSEGGKP 300
Query: 301 NPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSPTNNSLQTL 360
NPTASF++LFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIES LI INGSPTNNSL+TL
Sbjct: 301 NPTASFISLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESALIVINGSPTNNSLRTL 360
Query: 361 LNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKIFDLE 420
LNRKSQS+GSFKIKSDYVQQP+PL AI+GIWERLKSQDVKATTLDIVPYGGKMCKIFD E
Sbjct: 361 LNRKSQSVGSFKIKSDYVQQPMPLDAIKGIWERLKSQDVKATTLDIVPYGGKMCKIFDFE 420
Query: 421 TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD 480
TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD
Sbjct: 421 TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD 480
Query: 481 IGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTLSPP 536
IGTNTEYGKTSH+QASIWGFKYF KNFNRLVHVKTKVDPYD FRHEQSIP LSPP
Sbjct: 481 IGTNTEYGKTSHDQASIWGFKYFDKNFNRLVHVKTKVDPYDFFRHEQSIPILSPP 534
BLAST of Chy12G210320 vs. ExPASy TrEMBL
Match:
A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)
HSP 1 Score: 760.8 bits (1963), Expect = 3.9e-216
Identity = 372/545 (68.26%), Postives = 444/545 (81.47%), Query Frame = 0
Query: 1 MKHSSPLTPL--TVAFILLSPYPLWVVASSNNYKHQAFLQCL--------SSITRVIYTP 60
MK+SSPL PL ++ FI LS LW AS + KH++FL CL SI++V+YTP
Sbjct: 1 MKYSSPLIPLSFSLLFIFLSSSSLWAAASVD--KHESFLNCLLDHSPDSNYSISKVVYTP 60
Query: 61 INFSYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGH 120
+N SY SVLDFSI+NLRFS +TPKP+ IITP HVS +Q A++CSRTHG LQIRTRSGGH
Sbjct: 61 LNSSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHG-LQIRTRSGGH 120
Query: 121 DFEGLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAG 180
DFEGLS V++ PFII+DLINLRSI+ID NNTAWVQSGAT+GELYY IAEKSRTLAFPAG
Sbjct: 121 DFEGLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAG 180
Query: 181 VCPSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGG 240
CP+VGIGG SGGGYG +LRK+GLA DNVIDAYLVDANG+ DRE MGEDLFWAIRGGG
Sbjct: 181 ACPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGG 240
Query: 241 GGSFGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNG 300
GGSFGIVVAWK++LV +PATVT C NRT ++GAI+LI+ WQYVA+KLDENL LGILL G
Sbjct: 241 GGSFGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMG 300
Query: 301 GNISSEGG-KPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEIN 360
GN +++GG K NPTASF +LFLGKA++ +ILN TFP+LG+ K++C ++SWIES L+
Sbjct: 301 GNFTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA- 360
Query: 361 GSPTNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPY 420
G +SL+ LLNR + GS KIKSDYV++PI I GIWERLKS+DV A L VPY
Sbjct: 361 GFKIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPY 420
Query: 421 GGKMCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFP 480
GG+M +I + E PF HRAGNLY IGY+V W+ Q+ ++E+RHL WIRE+Y+YMTPFVSK P
Sbjct: 421 GGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSP 480
Query: 481 RAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSI 535
R+AYVNYRDLDIG+N +YGKTS ++AS+WG+KYFG NFNRLVHVKTKVDPYD FRHEQSI
Sbjct: 481 RSAYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSI 540
BLAST of Chy12G210320 vs. ExPASy TrEMBL
Match:
A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)
HSP 1 Score: 754.2 bits (1946), Expect = 3.7e-214
Identity = 362/540 (67.04%), Postives = 440/540 (81.48%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSS-------ITRVIYTPINF 60
MK+SSPL P ++ IL+S WV AS +KHQ FLQCLS I++V++TPIN
Sbjct: 1 MKYSSPLIPFSLILILVSSSSSWVSASP--HKHQNFLQCLSEHSSKRYPISKVVHTPINS 60
Query: 61 SYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 120
SY SVLDFSI+NL FSKPETPKP+ IITP+HVS IQ A+ICS++HG LQIRTRSGGHDFE
Sbjct: 61 SYSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHG-LQIRTRSGGHDFE 120
Query: 121 GLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCP 180
GLSYVAYHPFII+DLINLRSI IDV+ NTAWV+SGAT+GELYY+I EKSRTLAFP GVCP
Sbjct: 121 GLSYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCP 180
Query: 181 SVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGS 240
+VG+GG SGGGYG +LRK+GLA DNVIDAYLVDANG+VHDR+SMGEDLFWAIRGGGGGS
Sbjct: 181 TVGVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGS 240
Query: 241 FGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNI 300
FGIVVAWK++LVS+PATVTIC +NR K I+L+++WQYVA+KLDENL LGI+L GGNI
Sbjct: 241 FGIVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNI 300
Query: 301 -SSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSP 360
+++ G NP A+F +LFLGK + L++ L+ FP+LG TK+DC +TSWIESTLI G
Sbjct: 301 TTTQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVG 360
Query: 361 TNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGK 420
T SL+ LLNR ++ + K+KSDY ++PI + I G+W+RLK++D++ + L +PYGG+
Sbjct: 361 TVESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGR 420
Query: 421 MCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAA 480
M +I ++ETPF HRAG LY IGY V W+ QS + +++H+SWIR+IY YMTPFVSK PR+A
Sbjct: 421 MSQISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSA 480
Query: 481 YVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTL 533
Y NYRDLDIG N +YGKTS QASIWG KYFG NF RLV+VKTKVDPYD FRHEQSIPTL
Sbjct: 481 YANYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537
BLAST of Chy12G210320 vs. ExPASy TrEMBL
Match:
A0A1S3BDZ5 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)
HSP 1 Score: 754.2 bits (1946), Expect = 3.7e-214
Identity = 362/540 (67.04%), Postives = 440/540 (81.48%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSS-------ITRVIYTPINF 60
MK+SSPL P ++ IL+S WV AS +KHQ FLQCLS I++V++TPIN
Sbjct: 1 MKYSSPLIPFSLILILVSSSSSWVSASP--HKHQNFLQCLSEHSSKRYPISKVVHTPINS 60
Query: 61 SYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 120
SY SVLDFSI+NL FSKPETPKP+ IITP+HVS IQ A+ICS++HG LQIRTRSGGHDFE
Sbjct: 61 SYSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHG-LQIRTRSGGHDFE 120
Query: 121 GLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCP 180
GLSYVAYHPFII+DLINLRSI IDV+ NTAWV+SGAT+GELYY+I EKSRTLAFP GVCP
Sbjct: 121 GLSYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCP 180
Query: 181 SVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGS 240
+VG+GG SGGGYG +LRK+GLA DNVIDAYLVDANG+VHDR+SMGEDLFWAIRGGGGGS
Sbjct: 181 TVGVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGS 240
Query: 241 FGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNI 300
FGIVVAWK++LVS+PATVTIC +NR K I+L+++WQYVA+KLDENL LGI+L GGNI
Sbjct: 241 FGIVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNI 300
Query: 301 -SSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSP 360
+++ G NP A+F +LFLGK + L++ L+ FP+LG TK+DC +TSWIESTLI G
Sbjct: 301 TTTQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVG 360
Query: 361 TNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGK 420
T SL+ LLNR ++ + K+KSDY ++PI + I G+W+RLK++D++ + L +PYGG+
Sbjct: 361 TVESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGR 420
Query: 421 MCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAA 480
M +I ++ETPF HRAG LY IGY V W+ QS + +++H+SWIR+IY YMTPFVSK PR+A
Sbjct: 421 MSQISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSA 480
Query: 481 YVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTL 533
Y NYRDLDIG N +YGKTS QASIWG KYFG NF RLV+VKTKVDPYD FRHEQSIPTL
Sbjct: 481 YANYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537
BLAST of Chy12G210320 vs. ExPASy TrEMBL
Match:
A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)
HSP 1 Score: 753.4 bits (1944), Expect = 6.3e-214
Identity = 365/542 (67.34%), Postives = 442/542 (81.55%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCL---------SSITRVIYTPI 60
M + SPL L++AFI LS LW A N KH++FLQCL SI++VI+TPI
Sbjct: 1 MNYPSPLISLSLAFIFLSSSSLWASAFPN--KHESFLQCLLDHSLEYSNYSISKVIHTPI 60
Query: 61 NFSYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHD 120
N SY SVLDFSI+NLRFS ETPKP+ I+TP+HVS IQ A+ICS+++G QIRTRSGGHD
Sbjct: 61 NSSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYG-FQIRTRSGGHD 120
Query: 121 FEGLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGV 180
FEGLSYVA+ PFII+DLINLRSISID+K+NTAWVQSGAT+GELYY IA+KSRTLAFPAG+
Sbjct: 121 FEGLSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGI 180
Query: 181 CPSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGG 240
CP+VG+GG SGGGYG LLRKYGLA DNVIDAYLVDA+G+ H+RESMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGG 240
Query: 241 GSFGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGG 300
GSFGIVVAWK++LV +PATVT+C +NRT ++GA+++ + WQYVA+KLDENL LGIL GG
Sbjct: 241 GSFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGG 300
Query: 301 NISS-EGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEING 360
I+S EGGK NP A+F +LFLGKA++LLSILN FP+L + KKDC +TSWIEST++ G
Sbjct: 301 KITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVG 360
Query: 361 SPTNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYG 420
PT +L+ LL+R + S KIKSDYV++P+ ++GIW+RL SQD++ + VPYG
Sbjct: 361 FPTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYG 420
Query: 421 GKMCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPR 480
G+M +I + ETPF HRAG LY IGY+ GWE Q + E+RHL WIRE+Y+YMTPFVSK PR
Sbjct: 421 GRMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPR 480
Query: 481 AAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIP 533
AAY NYRDLDIG+N ++GKTS++QAS+WG KYFG NFNRLVHVKTKVDPYD FRHEQSIP
Sbjct: 481 AAYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 539
BLAST of Chy12G210320 vs. NCBI nr
Match:
XP_004135740.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KGN66416.2 hypothetical protein Csa_023239 [Cucumis sativus])
HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 525/535 (98.13%), Postives = 532/535 (99.44%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSSITRVIYTPINFSYFSVLD 60
MK SS LTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSSIT+VIYTPINFSYFSVLD
Sbjct: 1 MKRSSSLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSSITKVIYTPINFSYFSVLD 60
Query: 61 FSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAY 120
FSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVA+
Sbjct: 61 FSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAH 120
Query: 121 HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGF 180
HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGF
Sbjct: 121 HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGF 180
Query: 181 ISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAW 240
ISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDR+SMGEDLFWAIRGGGGGSFGIVVAW
Sbjct: 181 ISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRKSMGEDLFWAIRGGGGGSFGIVVAW 240
Query: 241 KLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNISSEGGKP 300
KLRLVS+PATVTICISNRT KDGAI+LIYEWQYVADKLDENLHLGILLNGGNISSEGGKP
Sbjct: 241 KLRLVSVPATVTICISNRTLKDGAIKLIYEWQYVADKLDENLHLGILLNGGNISSEGGKP 300
Query: 301 NPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSPTNNSLQTL 360
NPTASFL+LFLGKANKLLSILNKTFP+LGVTKKDCTQTSWIESTLIEINGSPTNNSLQTL
Sbjct: 301 NPTASFLSLFLGKANKLLSILNKTFPKLGVTKKDCTQTSWIESTLIEINGSPTNNSLQTL 360
Query: 361 LNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKIFDLE 420
LNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKIFDLE
Sbjct: 361 LNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKIFDLE 420
Query: 421 TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD 480
TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD
Sbjct: 421 TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD 480
Query: 481 IGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTLSPP 535
IGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTLSPP
Sbjct: 481 IGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTLSPP 535
BLAST of Chy12G210320 vs. NCBI nr
Match:
XP_008445743.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])
HSP 1 Score: 1018 bits (2632), Expect = 0.0
Identity = 499/535 (93.27%), Postives = 519/535 (97.01%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSSITRVIYTPINFSYFSVLD 60
MK SSPL PLT++FILLSPYPLWVVASSNN K QAFLQCLSSI++VIY+PIN SYFSVLD
Sbjct: 1 MKLSSPLIPLTLSFILLSPYPLWVVASSNN-KPQAFLQCLSSISKVIYSPINSSYFSVLD 60
Query: 61 FSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAY 120
FSIQNLRFSKPETPKPIAIITP HVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVA+
Sbjct: 61 FSIQNLRFSKPETPKPIAIITPIHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAH 120
Query: 121 HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGF 180
HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSR+LAFPAGVCPSVGIGGF
Sbjct: 121 HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRSLAFPAGVCPSVGIGGF 180
Query: 181 ISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAW 240
+SGGGYGYLLRKYGLAVDNV+DAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAW
Sbjct: 181 VSGGGYGYLLRKYGLAVDNVLDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAW 240
Query: 241 KLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNISSEGGKP 300
KLRLV +P TVTICISN+T KDGAI+LIYEWQYVADKLDENL+LGILLNGGNISSEGGKP
Sbjct: 241 KLRLVLVPETVTICISNKTVKDGAIDLIYEWQYVADKLDENLYLGILLNGGNISSEGGKP 300
Query: 301 NPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSPTNNSLQTL 360
NPTASF++LFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIES LI INGSPTNNSL+TL
Sbjct: 301 NPTASFISLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESALIVINGSPTNNSLRTL 360
Query: 361 LNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKIFDLE 420
LNRKSQS+GSFKIKSDYVQQP+PL AI+GIWERLKSQDVKATTLDIVPYGGKMCKIFD E
Sbjct: 361 LNRKSQSVGSFKIKSDYVQQPMPLDAIKGIWERLKSQDVKATTLDIVPYGGKMCKIFDFE 420
Query: 421 TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD 480
TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD
Sbjct: 421 TPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLD 480
Query: 481 IGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTLSPP 535
IGTNTEYGKTSH+QASIWGFKYF KNFNRLVHVKTKVDPYD FRHEQSIP LSPP
Sbjct: 481 IGTNTEYGKTSHDQASIWGFKYFDKNFNRLVHVKTKVDPYDFFRHEQSIPILSPP 534
BLAST of Chy12G210320 vs. NCBI nr
Match:
XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])
HSP 1 Score: 756 bits (1953), Expect = 2.28e-270
Identity = 364/539 (67.53%), Postives = 442/539 (82.00%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSS-------ITRVIYTPINF 60
MK SSPL PL + I+L PLW AS Y+H FLQCLS I++V+Y+PIN
Sbjct: 1 MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQH--FLQCLSEHSSNSYPISKVVYSPINS 60
Query: 61 SYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 120
SY SVLDFSI+NLRFSKP+TPKP+ IITP+HVS IQ A+ICS++HG LQIRTRSGGHDFE
Sbjct: 61 SYSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHG-LQIRTRSGGHDFE 120
Query: 121 GLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCP 180
GLSYVAY PFI++DLINLRSIS+D +NNTAWV+SGAT+GELYY+I EKSRTLAFPAGVCP
Sbjct: 121 GLSYVAYRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCP 180
Query: 181 SVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGS 240
+VGIGG SGGGYG +LRK+GLA DNVIDA+LVDANG+VHDRESMGEDLFWAIRGGGGGS
Sbjct: 181 TVGIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGS 240
Query: 241 FGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNI 300
FGIVVAWK++LV +PATVTIC + R ++ AI+L++ WQYVA+KLDENL+LGI+L GG
Sbjct: 241 FGIVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKA 300
Query: 301 SSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSPT 360
S++GG NPTA+F +LFLG+ ++LL+ L+ TFP+LG+ K+DC + +W+ESTLI G
Sbjct: 301 STQGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQP 360
Query: 361 NNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKM 420
SL+ LLNR +I + KIKSDYV++P+P AI GI +RLK+QD++ + L VPYGGKM
Sbjct: 361 IESLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKM 420
Query: 421 CKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAY 480
+I + ETPF HRAGNLY IGY+VGW+ QS + ++RH+ WIRE+Y YM PFVSK PRAAY
Sbjct: 421 SQISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAY 480
Query: 481 VNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTL 532
NYRDLDIG N +YG+TS EQAS+WG KYFG NF +LV+VKTKVDPYD FRHEQSIPTL
Sbjct: 481 ANYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536
BLAST of Chy12G210320 vs. NCBI nr
Match:
XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])
HSP 1 Score: 752 bits (1942), Expect = 1.34e-268
Identity = 372/545 (68.26%), Postives = 444/545 (81.47%), Query Frame = 0
Query: 1 MKHSSPLTPLTVA--FILLSPYPLWVVASSNNYKHQAFLQCLS--------SITRVIYTP 60
MK+SSPL PL+ + FI LS LW AS + KH++FL CL SI++V+YTP
Sbjct: 1 MKYSSPLIPLSFSLLFIFLSSSSLWAAASVD--KHESFLNCLLDHSPDSNYSISKVVYTP 60
Query: 61 INFSYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGH 120
+N SY SVLDFSI+NLRFS +TPKP+ IITP HVS +Q A++CSRTHG LQIRTRSGGH
Sbjct: 61 LNSSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHG-LQIRTRSGGH 120
Query: 121 DFEGLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAG 180
DFEGLS V++ PFII+DLINLRSI+ID NNTAWVQSGAT+GELYY IAEKSRTLAFPAG
Sbjct: 121 DFEGLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAG 180
Query: 181 VCPSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGG 240
CP+VGIGG SGGGYG +LRK+GLA DNVIDAYLVDANG+ DRE MGEDLFWAIRGGG
Sbjct: 181 ACPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGG 240
Query: 241 GGSFGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNG 300
GGSFGIVVAWK++LV +PATVT C NRT ++GAI+LI+ WQYVA+KLDENL LGILL G
Sbjct: 241 GGSFGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMG 300
Query: 301 GNISSEGG-KPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEIN 360
GN +++GG K NPTASF +LFLGKA++ +ILN TFP+LG+ K++C ++SWIES L+
Sbjct: 301 GNFTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA- 360
Query: 361 GSPTNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPY 420
G +SL+ LLNR + GS KIKSDYV++PI I GIWERLKS+DV A L VPY
Sbjct: 361 GFKIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPY 420
Query: 421 GGKMCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFP 480
GG+M +I + E PF HRAGNLY IGY+V W+ Q+ ++E+RHL WIRE+Y+YMTPFVSK P
Sbjct: 421 GGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSP 480
Query: 481 RAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSI 534
R+AYVNYRDLDIG+N +YGKTS ++AS+WG+KYFG NFNRLVHVKTKVDPYD FRHEQSI
Sbjct: 481 RSAYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSI 540
BLAST of Chy12G210320 vs. NCBI nr
Match:
XP_004151192.3 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical protein Csa_023212 [Cucumis sativus])
HSP 1 Score: 751 bits (1940), Expect = 2.24e-268
Identity = 361/540 (66.85%), Postives = 446/540 (82.59%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSS-------ITRVIYTPINF 60
MK+SSPL P ++ I++S LWV S++ +KH FLQCLS I++V++TPIN
Sbjct: 1 MKYSSPLIPFSLILIVVSSSSLWV--SASPHKHHNFLQCLSEHSSKSYPISKVVHTPINS 60
Query: 61 SYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 120
SY SVL+FSI+NLRFSKPETPKP+ IITP+HVS IQ A+ICS++HG LQIRTRSGGHD+E
Sbjct: 61 SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHG-LQIRTRSGGHDYE 120
Query: 121 GLSYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCP 180
GLSYVA HPFIILDLINLRS+ IDV++NTAWV+SG T+GELYY+I EKSRTLAFPAG+CP
Sbjct: 121 GLSYVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICP 180
Query: 181 SVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGS 240
+VG+GG SGGGYG +LRK+GLA DNVIDAYLVDANG+VHDRESMGEDLFWAIRGGGGGS
Sbjct: 181 TVGVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGS 240
Query: 241 FGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNI 300
FGIVVAWK++LVS+PATVT+C + R K I+L+++WQYVA+KLDENL LGI+L GGNI
Sbjct: 241 FGIVVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNI 300
Query: 301 SS-EGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSP 360
++ + G NP A+F +LFLGK N+L++ L+ TFP+LG+ K+DC +TSWIESTLI G
Sbjct: 301 TTTQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQ 360
Query: 361 TNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGK 420
T SL+ LLNR ++ + KIKSDY+++PI + I GIW+RLK+QD++ + L +PYGG+
Sbjct: 361 TVESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGR 420
Query: 421 MCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAA 480
M +I + ETPF HR GNLY IGY++GW+ QS + +++H+SWIREIY YMTPFVSK PR+A
Sbjct: 421 MSQISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSA 480
Query: 481 YVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTL 532
Y NYRDLDIG N +YGKTS +QASIWG KYFG NF RLV+VKTKVDPYD FRHEQSIPTL
Sbjct: 481 YANYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537
BLAST of Chy12G210320 vs. TAIR 10
Match:
AT4G20820.1 (FAD-binding Berberine family protein )
HSP 1 Score: 526.2 bits (1354), Expect = 3.1e-149
Identity = 262/537 (48.79%), Postives = 365/537 (67.97%), Query Frame = 0
Query: 4 SSPLTPLTVAFILLS-PYPLWVVASSNNYKHQAFLQCLS-------SITRVIYTPINFSY 63
SS L T + +LLS P+P+ S+N +FLQCLS +++VI+TP + S+
Sbjct: 8 SSVLIFFTTSTLLLSIPHPV----SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 67
Query: 64 FSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGL 123
SVL SIQN RFS P+ PKP+ I+TP S +Q A+ C+R G + IRTRSGGHD+EGL
Sbjct: 68 SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFG-IHIRTRSGGHDYEGL 127
Query: 124 SYVAYHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSV 183
SYV + PF+ILDL NLRSI++DV N + WVQ+GAT+GELYY+I +K+RTLAFPAGVCP+V
Sbjct: 128 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTV 187
Query: 184 GIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFG 243
G+GG SGGGYG LLRK+GLA D+VIDA +VDA G + +R MGED FWAIRGGGG SF
Sbjct: 188 GVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFC 247
Query: 244 IVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNISS 303
+V++WK+ L+++P+TVT+ + + A+++I+ WQ+VADK+ ++L + ++L
Sbjct: 248 VVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML------- 307
Query: 304 EGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTL--IEINGSPT 363
+ K ASF L+LG LL ++NK FP+LG+ + DCT+ SWIES + E+ P
Sbjct: 308 QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPI 367
Query: 364 NNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKM 423
N +L +++++ +FK KSD+VQ+P+P AI +W RL+ + + L P+GGKM
Sbjct: 368 N-----VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKM 427
Query: 424 CKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAY 483
+I D ETPFPHR GN+Y I YL W +++E+++ W+ +Y+ M+ FV+K PR AY
Sbjct: 428 SEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAY 487
Query: 484 VNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIP 531
+N RDLD+G ++ +E+ WG KYF NF RLV VKT VDP D F EQSIP
Sbjct: 488 INLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524
BLAST of Chy12G210320 vs. TAIR 10
Match:
AT5G44440.1 (FAD-binding Berberine family protein )
HSP 1 Score: 524.6 bits (1350), Expect = 9.1e-149
Identity = 262/544 (48.16%), Postives = 368/544 (67.65%), Query Frame = 0
Query: 1 MKHSSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLS-------SITRVIYTPINF 60
M+ SS L + + + +SP + S++ H+ FL+CLS +VI+T +
Sbjct: 1 MEFSSFLFTILLFSLNISP-----LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDS 60
Query: 61 SYFSVLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 120
S+FS+LD SIQN RFS ETPKP++IITP S +Q I C++ HG + +RTRS GH +E
Sbjct: 61 SFFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHG-IHVRTRSAGHCYE 120
Query: 121 GLSYVAYH-PFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVC 180
GLSY+AY+ PF ++DL NLRSIS+DV N T WVQ+GAT GELYY+I + +++LAFPAG+
Sbjct: 121 GLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIH 180
Query: 181 PSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGG 240
P+VG+GG SGGGYG LLRKYGLA DN+IDA +VDA+G + DR++MGED FWAIRGGGG
Sbjct: 181 PTVGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGS 240
Query: 241 SFGIVVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGN 300
SFG++++WK++LV +P+T+T+ +T K A+ +I +WQY ADK+ ++L + L N
Sbjct: 241 SFGVILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN 300
Query: 301 ISSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSP 360
K A F L++G N LL+++ + FP+LG+ K+ C + SWIES L P
Sbjct: 301 ------KNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVL-WFADFP 360
Query: 361 TNNSLQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGK 420
SL L NR+ S+ SFK K D+VQ+PIP AI+ IW RL++ + + + + P+GGK
Sbjct: 361 KGESLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGK 420
Query: 421 MCKIFDLETPFPHRAGNLYMIGYLVGW---ENQSKEIEERHLSWIREIYNYMTPFVSKFP 480
M ++ + ETPFPHR GNLY I Y+ W E+++K +++L W+ +Y +MTP+VSK P
Sbjct: 421 MSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSP 480
Query: 481 RAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSI 534
R AYVN++D+D+G KT +E+ WG KYF NF RLV VKT+VDP D F EQSI
Sbjct: 481 RGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSI 530
BLAST of Chy12G210320 vs. TAIR 10
Match:
AT5G44410.1 (FAD-binding Berberine family protein )
HSP 1 Score: 511.9 bits (1317), Expect = 6.1e-145
Identity = 263/540 (48.70%), Postives = 361/540 (66.85%), Query Frame = 0
Query: 7 LTPLTVAFIL----LSPYPLWVVASSNNYKHQAFLQCLS-----SITRVIYTPINFSYFS 66
L L + F+L LS +P S+ H+ FL+CLS +R+I+T + SYFS
Sbjct: 7 LLSLFIYFLLLNLSLSHFP---SISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFS 66
Query: 67 VLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSY 126
+L+ SIQN RF ETPKP++IITP + +Q I C+R HG + IRTRSGGHD+EGLSY
Sbjct: 67 ILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHG-IHIRTRSGGHDYEGLSY 126
Query: 127 VA-YHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVG 186
+A PF+++DL NLRSI++DV N T WVQSGAT+GELYY+I + S++LAFPAG+ P+VG
Sbjct: 127 MAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVG 186
Query: 187 IGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGI 246
IGG GGGYG L+RKYGL+ DNVIDA++VDANG DR+ MGED FWAIRGGGG SF +
Sbjct: 187 IGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSV 246
Query: 247 VVAWKLRLVSIPATVTICISNRTQKDGAIELIYEWQYVADKLDENLHLGILLNGGNISSE 306
V++WK+RL+ +P+ VT+ +T + A+ +I +WQY+ADK+ +L + +L
Sbjct: 247 VLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ------- 306
Query: 307 GGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEINGSPTNNS 366
+ ASF L+LG + LL+++ FP+LG+ +C + SWIES L I G S
Sbjct: 307 -KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKG----ES 366
Query: 367 LQTLLNRKSQSIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPYGGKMCKI 426
++ L RK S SFK K D++++PIP AI+ +W R ++ + + + + P+GGKM +I
Sbjct: 367 MEILAKRKRTS-RSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 426
Query: 427 FDLETPFPHRAGNLYMIGYLVGW---ENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAY 486
D E PFPHR GNLY I YL W E+++K E++L W+ +Y +MTP+VSK PR AY
Sbjct: 427 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 486
Query: 487 VNYRDLDIGTNTEYG-KTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSIPTL 533
VN+RD+D+G KT +E+A +WG KYF NF+RLV VKT VDP D F EQSIP +
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529
BLAST of Chy12G210320 vs. TAIR 10
Match:
AT5G44400.1 (FAD-binding Berberine family protein )
HSP 1 Score: 506.9 bits (1304), Expect = 2.0e-143
Identity = 260/543 (47.88%), Postives = 363/543 (66.85%), Query Frame = 0
Query: 4 SSPLTPLTVAFILLSPYPLWVVASSNNYKHQAFLQCLSSITRVIYTPINFSYFS------ 63
S PL ++ + S Y + +S + + Q F+ C+ T V Y P+ ++F+
Sbjct: 4 SKPLPLFSILVLYFSLYTITPTSSLASLQDQ-FINCVQRNTHV-YFPLEKTFFAPTKNVS 63
Query: 64 ----VLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFE 123
VL+ + QNLRF K PKP I +P H S +Q +IICS+ + +R RSGGHD+E
Sbjct: 64 MFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKL-RMHLRVRSGGHDYE 123
Query: 124 GLSYVAY--HPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGV 183
GLSYV+ PFI++DL +R ++I++++N+AWVQSGATVGELYY+IAEKS+ FPAG+
Sbjct: 124 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 183
Query: 184 CPSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGG 243
C S+GIGG I+GG YG ++RKYGL DNV+DA +VDANG++ DR +MGED FWAIRGG G
Sbjct: 184 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 243
Query: 244 GSFGIVVAWKLRLVSIPATVTICISNRT-QKDGAIELIYEWQYVADKLDENLHLGILLNG 303
GSFGI++AWK++LV +P TVT+ +T Q+D ++I +WQ VADKL E L + +L
Sbjct: 244 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF-- 303
Query: 304 GNISSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTLIEING 363
N++ GG T S+ LFLG L++++ K+FP+LG+T KDC + SW+ES + I+G
Sbjct: 304 -NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLES-IAYISG 363
Query: 364 SPTNNSLQTLLNRKSQ-SIGSFKIKSDYVQQPIPLVAIRGIWERLKSQDVKATTLDIVPY 423
PT+ LL KS SFK KSD+V+ PIP ++GI+++L +D+ + PY
Sbjct: 364 FPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWN--PY 423
Query: 424 GGKMCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTPFVSKFP 483
GG M KI + + PFPHR G L+ + Y+ W + K RH++WIR++Y+YMTP+VS P
Sbjct: 424 GGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKR-PSRHINWIRDLYSYMTPYVSSNP 483
Query: 484 RAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLFRHEQSI 533
R AYVNYRDLD+G NT+ KT +QA +WG YF NFNRL+ +K KVDP + FRHEQSI
Sbjct: 484 REAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSI 536
BLAST of Chy12G210320 vs. TAIR 10
Match:
AT5G44380.1 (FAD-binding Berberine family protein )
HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-141
Identity = 265/549 (48.27%), Postives = 364/549 (66.30%), Query Frame = 0
Query: 1 MKHSSPLTPLT---VAFILLSPYPLWVVASSNNYKHQAFLQCLSSITRV-------IYTP 60
M +S PL ++ V+ + S Y + +SS + + Q F+ C+ T V ++TP
Sbjct: 1 MGNSKPLPTISCIIVSVLYFSFYCITPTSSSASIQDQ-FINCVKRNTHVSFPLEKTLFTP 60
Query: 61 I-NFSYFS-VLDFSIQNLRFSKPETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSG 120
N S F+ VL+ + QNL+F PKP I P H SQ+Q +IICS+ G + R RSG
Sbjct: 61 AKNVSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLG-IHFRVRSG 120
Query: 121 GHDFEGLSYVA--YHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLA 180
GHDFE LSYV+ PFI+LDL L+ I++D+++N+AWVQ GAT+GELYY+IAEKS+
Sbjct: 121 GHDFEALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHG 180
Query: 181 FPAGVCPSVGIGGFISGGGYGYLLRKYGLAVDNVIDAYLVDANGEVHDRESMGEDLFWAI 240
FPAG+C SVGIGG+++GGGYG L+RKYGLA DNV+D +VDANG++ DR +MGEDLFWAI
Sbjct: 181 FPAGLCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAI 240
Query: 241 RGGGGGSFGIVVAWKLRLVSIPATVTICISNRT-QKDGAIELIYEWQYVADKLDENLHLG 300
RGGGG SFGIV+AWK++LV +P TVT+ +T ++D ++ I +WQ ++ K+ E +H+
Sbjct: 241 RGGGGASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIR 300
Query: 301 ILLNGGNISSEGGKPNPTASFLTLFLGKANKLLSILNKTFPQLGVTKKDCTQTSWIESTL 360
++L + G T ++L FLG+ LL ++ K FP+LG+T+KDCT+ SWIE+ L
Sbjct: 301 VVLRA---AGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAAL 360
Query: 361 IEINGSPTNNSLQTLLNRKSQSIGS--FKIKSDYVQQPIPLVAIRGIWERLKSQDVKATT 420
G PT + ++ LL KS +G FK SD+V++PIP++ ++GI++RL + T
Sbjct: 361 FH-GGFPTGSPIEILLQLKS-PLGKDYFKATSDFVKEPIPVIGLKGIFKRL--IEGNTTF 420
Query: 421 LDIVPYGGKMCKIFDLETPFPHRAGNLYMIGYLVGWENQSKEIEERHLSWIREIYNYMTP 480
L+ PYGG M KI + PFPHR G L+ I Y W K R ++WI+EIYNYM P
Sbjct: 421 LNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANWLENDK-TSSRKINWIKEIYNYMAP 480
Query: 481 FVSKFPRAAYVNYRDLDIGTNTEYGKTSHEQASIWGFKYFGKNFNRLVHVKTKVDPYDLF 533
+VS PR AYVNYRDLD G N K + +A IWG KYF NF+RLV +KTKVDP + F
Sbjct: 481 YVSSNPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFF 539
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9SVG5 | 4.4e-148 | 48.79 | Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... | [more] |
| Q9FI21 | 1.3e-147 | 48.16 | Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... | [more] |
| Q9FI25 | 8.6e-144 | 48.70 | Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=... | [more] |
| Q9FKU8 | 2.8e-142 | 47.88 | Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... | [more] |
| A6P6V9 | 3.6e-142 | 50.59 | Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S3BDG0 | 3.9e-296 | 93.27 | cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488675 PE=3 SV... | [more] |
| A0A6J1DHZ2 | 3.9e-216 | 68.26 | berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 P... | [more] |
| A0A5D3C1A1 | 3.7e-214 | 67.04 | Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... | [more] |
| A0A1S3BDZ5 | 3.7e-214 | 67.04 | tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863... | [more] |
| A0A6J1CN68 | 6.3e-214 | 67.34 | tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC1... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_004135740.2 | 0.0 | 98.13 | berberine bridge enzyme-like 18 [Cucumis sativus] >KGN66416.2 hypothetical prote... | [more] |
| XP_008445743.1 | 0.0 | 93.27 | PREDICTED: cannabidiolic acid synthase-like [Cucumis melo] | [more] |
| XP_038895543.1 | 2.28e-270 | 67.53 | berberine bridge enzyme-like 18 [Benincasa hispida] | [more] |
| XP_022152516.1 | 1.34e-268 | 68.26 | berberine bridge enzyme-like 18 [Momordica charantia] | [more] |
| XP_004151192.3 | 2.24e-268 | 66.85 | berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical pro... | [more] |
