Chy12G210300 (gene) Cucumber (hystrix) v1

Overview
NameChy12G210300
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionberberine bridge enzyme-like 18
LocationchrH12: 5208547 .. 5211335 (-)
RNA-Seq ExpressionChy12G210300
SyntenyChy12G210300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAGTCTTCTCCATCAATCCATCTTTCTCTAATTGCTTTCATTGTTATATGTTCATCATTTTCATTATCGAATGCAGCTTCTTCTGACAAACATGAAGAGTTTCTTCAATGTCTAATTCATCACTCTCCACATAGTTCATCTATTACCAAACTTATTTACACTCAAAACAACTCCTCCTATTCCTCAGTCCTAAACTTGTCCATTAGAAACCACAGATTCTCAACTCCCAACACCCCAAAACCACTCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCTGTCATTTGCTCCAAGTCTCAAGGCCTTCAAATCCGAATCCGAAGCGGTGGTCACGACTTCGAGGGTCTTTCTTACGTTGCCTACCTACCATTCATCGTTGTTGATCTTATTAATCTAAGGTCTATCACGGTCAACGTCGAAAGAAGAACCGCATGGGTTCAGTCAGCGGCAACTCTAGGTGAACTTTACTACAGAATTGCTGAAAAAAGTCCAACCTTGACCTTTCCGGGCGGGGCTTGTCCAACTGTTTGTTTTGGTGGGTACCTTAGTGGTGGGGGATATGGGCTGTTGCTGAGGAAATATGGTGTTGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAACTGGGGAGTTTCATGATAGGGAATCGATGGGCGAGGATTTGTTTTGGGCAATTAGAGGCGGCGGAGGAGGGAGCTTTGGAATTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCGGCGACGGTGACGTTTTGCTCAAGTAGCAGAACTTTCGAAGAAGATGCAATCAATCTAATCCATCAGTGGCAATATGTGGGTTACAAATTGGAAAATAATATAGCCCATGGAGTCCTTACCATTGGTATTTCTCTTTCAACGTATTTTTTATATTATATACAATTATTATTTTTTTTTGGAATAATTTGTTTATTTTTTCATCATTTAATTTTTTCACTGAAAATGATACTACACCAGATTAAAGATTGAAAACAATAAAAAGGAAATAGACTTTTGGGAAAAATACCTTTTTATCATAGAATAGGTGAATTTAGTTTTTTCATGTTTTAAACTTTTTTTTTTCACTCACACTTTGTCTTATTTCTCTTCCCATTTTCGTTCTTCTTCATTCATATTCCCTCCCCTCTCCTCCCTCCTTGGTTCTTCTTCTTTTCTCTCACCCTATTTTCTCTCCTCGTTCTCCATGCATAAACTTAGAAGGAGAACAAAAATGGAGGAGAGAGAATAGAATGTGAGACAAGGACGACGAAGATGGGGGAGAGAGAAAAATAAAAGAAAAATAAAAATAGCTTTTTTTTGCCATCTCTTGCTACCATCTAAGCGTTAGTCTAATTGTTTTTTCAAATTCTTGTCAAGGAATCATTAAACCATTAAAAACTCAAGCTAAGAAAGGGGTTTTCTCAAAAACAGAAAAAAAAAATGAAAATATTTACAAAATTGGTTCAAAAAAACAAAATTTATTAAATTTGATTTTTCTATGAAGTGTAATATTTTGTCACATATTCTATTTTTTATGATTTCTTTTTTTGATATGAAATTATAAAAAATAAGAAAAAAATAGATTATTTATAGAGGCATTTTTAAAAATAGCAAAATAAATTAAAATAGAGAAATTTTCAAAAATAGCAAAATTTTGGATTCTATCACTATAGCATATTAATGACTATCAATGATAGAATTTCCTATAGGTTGTAAATATTTTGTAAAATCTACTATTTTAAAAAATTCCCCTTATTTATACTCCTAAGAAAAATGAATAGTATTGAATTTTGTTTTTTAGTGGAGTGGAGTGGAGTGTAAGTAGTTTTTTACTCTTAGGAAAATAACTCTTTTTAAAATTTCATGGACTAAAAGATATTTTTTATAGATTTTTTTATCTAGGGTAGAATATAAATTTGATTTTTTCATTCTTTTGTACTTGAAATGGGTCTAATTTTAGTAATTATATCCTTTTAATAAATTTTAAATAGTTTAGAAAAAATTATTGTCGAGGAGAAGATTAAAAATATAACAAAATTTTAGATATTATACTAAATGTTTTAAACTTTTAAACTATTATTTATTTTTCTATTTTTATTATAAAGATAATGAAAGTGTGAGTTTTATTCTTTTTTTCTTTCTTTATTTCTTTTAACAAACAACATATTAGAGAAAAATGGGTCCAATATATTGCAACTTTAGATGCATTCTTAAAGTAAAGTTAGATCCATAAGTCGTTGTTTTTTACTTTTTTTTCTCACTTATTTTCTTTTAAATAAAGTTTGGACAATATAAATGGTGATTCCAATTTCTTCTTTTTTCTTTTCTTTTCTTTTCTTTTTCTTTCTTTTTTTTTTTTTTTTTGTCATGGATTTTAAGCAAGGAAGTCTACACAATTAAACTTTAATTTTAGTCTTTTCAATTAAAGCAATAAACTTATAGAAGTGGCAACATTATTACAATTTAATTTTGTTCACCTTTTCTTTTAATTTCCTGATGAATTTGAGCTAGGCATGGTTCCTCCTTCTTTCATTAGGATTGTTATTTATTTTAAGACCTTTAATTTCTTTGCTTTTATTCTACACATAATAGATTTGATTTGAGCAAATAAAATGCCAGGTTTGAAGATCGAATAATCTTCAACCTCTAAAAAAATCAAGTCAATTATTGTTAAAGAAAAGAAAGTGAATTTTGACTAGTTGTTTGAGATTTTGAATGTGGATTTATACATTTAGAATAATAGGATTTTGAGAGAGTTTACTGGGTAA

mRNA sequence

ATGAAGAAGTCTTCTCCATCAATCCATCTTTCTCTAATTGCTTTCATTGTTATATGTTCATCATTTTCATTATCGAATGCAGCTTCTTCTGACAAACATGAAGAGTTTCTTCAATGTCTAATTCATCACTCTCCACATAGTTCATCTATTACCAAACTTATTTACACTCAAAACAACTCCTCCTATTCCTCAGTCCTAAACTTGTCCATTAGAAACCACAGATTCTCAACTCCCAACACCCCAAAACCACTCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCTGTCATTTGCTCCAAGTCTCAAGGCCTTCAAATCCGAATCCGAAGCGGTGGTCACGACTTCGAGGGTCTTTCTTACGTTGCCTACCTACCATTCATCGTTGTTGATCTTATTAATCTAAGGTCTATCACGGTCAACGTCGAAAGAAGAACCGCATGGGTTCAGTCAGCGGCAACTCTAGGTGAACTTTACTACAGAATTGCTGAAAAAAGTCCAACCTTGACCTTTCCGGGCGGGGCTTGTCCAACTGTTTGTTTTGGTGGGTACCTTAGTGGTGGGGGATATGGGCTGTTGCTGAGGAAATATGGTGTTGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAACTGGGGAGTTTCATGATAGGGAATCGATGGGCGAGGATTTGTTTTGGGCAATTAGAGGCGGCGGAGGAGGGAGCTTTGGAATTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCGGCGACGGTGACGTTTTGCTCAAGTAGCAGAACTTTCGAAGAAGATGCAATCAATCTAATCCATCAGTGGCAATATGTGGGTTACAAATTGGAAAATAATATAGCCCATGGAGTCCTTACCATTGATAATGAAAGTAATAATAGGATTTTGAGAGAGTTTACTGGGTAA

Coding sequence (CDS)

ATGAAGAAGTCTTCTCCATCAATCCATCTTTCTCTAATTGCTTTCATTGTTATATGTTCATCATTTTCATTATCGAATGCAGCTTCTTCTGACAAACATGAAGAGTTTCTTCAATGTCTAATTCATCACTCTCCACATAGTTCATCTATTACCAAACTTATTTACACTCAAAACAACTCCTCCTATTCCTCAGTCCTAAACTTGTCCATTAGAAACCACAGATTCTCAACTCCCAACACCCCAAAACCACTCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCTGTCATTTGCTCCAAGTCTCAAGGCCTTCAAATCCGAATCCGAAGCGGTGGTCACGACTTCGAGGGTCTTTCTTACGTTGCCTACCTACCATTCATCGTTGTTGATCTTATTAATCTAAGGTCTATCACGGTCAACGTCGAAAGAAGAACCGCATGGGTTCAGTCAGCGGCAACTCTAGGTGAACTTTACTACAGAATTGCTGAAAAAAGTCCAACCTTGACCTTTCCGGGCGGGGCTTGTCCAACTGTTTGTTTTGGTGGGTACCTTAGTGGTGGGGGATATGGGCTGTTGCTGAGGAAATATGGTGTTGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAACTGGGGAGTTTCATGATAGGGAATCGATGGGCGAGGATTTGTTTTGGGCAATTAGAGGCGGCGGAGGAGGGAGCTTTGGAATTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCGGCGACGGTGACGTTTTGCTCAAGTAGCAGAACTTTCGAAGAAGATGCAATCAATCTAATCCATCAGTGGCAATATGTGGGTTACAAATTGGAAAATAATATAGCCCATGGAGTCCTTACCATTGATAATGAAAGTAATAATAGGATTTTGAGAGAGTTTACTGGGTAA

Protein sequence

MKKSSPSIHLSLIAFIVICSSFSLSNAASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVLTIDNESNNRILREFTG*
Homology
BLAST of Chy12G210300 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-81
Identity = 143/260 (55.00%), Postives = 194/260 (74.62%), Query Frame = 0

Query: 30  SDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPLLIITP 89
           ++  E FL+C   H P++ +  KL+YTQ++  Y S+LN +I+N RF +  TPKPL+I+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 90  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVNVERRTA 149
           SN SHIQA ++CSK  GLQIR RSGGHD EG+SY++ +PF+VVDL N+ SI ++V  +TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 150 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGVAADNVIDA 209
           WV++ ATLGE+YY I EK+  L+FPGG CPTV  GG+ SGGGYG L+R YG+AADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 210 YLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 269
           +LV+  G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 270 DAINLIHQWQYVGYKLENNI 289
             + L ++WQ + YK + ++
Sbjct: 268 GLVKLFNKWQNIAYKYDKDL 287

BLAST of Chy12G210300 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.3e-80
Identity = 141/284 (49.65%), Postives = 203/284 (71.48%), Query Frame = 0

Query: 11  SLIAFIVICS------SFSLSNAASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSS 70
           S  +F  +C       SF++  + ++ + E FL+C   + P++++  KL+YTQNN  Y S
Sbjct: 4   STFSFWFVCKIIFFFFSFNIQTSIANPR-ENFLKCFSQYIPNNATNLKLVYTQNNPLYMS 63

Query: 71  VLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYV 130
           VLN +I N RF++  TPKPL+I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 64  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 123

Query: 131 AYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFG 190
           + +PF++VDL N+RSI ++V  +TAWV++ ATLGE+YY + EK+  L+   G CPTVC G
Sbjct: 124 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 183

Query: 191 GYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVV 250
           G+  GGGYG L+R YG+AADN+IDA+LV+  G+  DR+SMGEDLFWA+RGGG  SFGI+V
Sbjct: 184 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 243

Query: 251 AWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNI 289
           AWK++LV VP +  F         + + L+++WQ + YK + ++
Sbjct: 244 AWKIRLVAVPKSTMFSVKKIMEIHELVKLVNKWQNIAYKYDKDL 286

BLAST of Chy12G210300 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 6.6e-80
Identity = 141/262 (53.82%), Postives = 196/262 (74.81%), Query Frame = 0

Query: 27  AASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPLLI 86
           +A+      FLQCL      S+ ++K+I+T N++S+SSVL  SI+N RFS P+ PKP+LI
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 87

Query: 87  ITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVNVER 146
           +TP   S +Q+AV C++  G+ IR RSGGHD+EGLSYV + PF+++DL NLRSITV+V+ 
Sbjct: 88  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 147

Query: 147 RTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGVAADNV 206
           R+ WVQ+ AT+GELYY I +K+ TL FP G CPTV  GG+ SGGGYG LLRK+G+AAD+V
Sbjct: 148 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 207

Query: 207 IDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTF 266
           IDA +VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT  + ++  
Sbjct: 208 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 267

Query: 267 EEDAINLIHQWQYVGYKLENNI 289
           E+ A+ +IH+WQ+V  K+ +++
Sbjct: 268 EQSALKIIHRWQFVADKVSDDL 289

BLAST of Chy12G210300 vs. ExPASy Swiss-Prot
Match: A0A2S1XB67 (O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 8.7e-80
Identity = 154/293 (52.56%), Postives = 206/293 (70.31%), Query Frame = 0

Query: 8   IHLSLIAFIVICSSFSLSNAASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLN 67
           I LS+  F+++ SS      +     E FL C+ +      SI  +++  +NSSY SVL 
Sbjct: 7   IVLSIFCFLLLLSS------SHGSIPEAFLNCISNKFSLDVSILNILHVPSNSSYDSVLK 66

Query: 68  LSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYL 127
            +I+N RF    +PKPL IITP   SH+Q+AVIC+K  GLQIRIRSGG D+EGLSY + +
Sbjct: 67  STIQNPRFL--KSPKPLAIITPVLHSHVQSAVICTKQAGLQIRIRSGGADYEGLSYRSEV 126

Query: 128 PFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYL 187
           PFI++DL NLRSI+V++E  +AWV+S AT+GE Y+ IA+ SP   FP G   +V  GG+L
Sbjct: 127 PFILLDLQNLRSISVDIEDNSAWVESGATIGEFYHEIAQNSPVHAFPAGVSSSVGIGGHL 186

Query: 188 SGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWK 247
           S GG+G LLRKYG+AADN+IDA +VDA G   DRESMGEDLFWAIRGGGG SFG++V+WK
Sbjct: 187 SSGGFGTLLRKYGLAADNIIDAKIVDARGRILDRESMGEDLFWAIRGGGGASFGVIVSWK 246

Query: 248 VKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVLTIDNESN 301
           VKLV VP  VT    S+T+EE  ++L+H+WQY+ +KL  ++   V  +D+ S+
Sbjct: 247 VKLVKVPPMVTVFILSKTYEEGGLDLLHKWQYIEHKLPEDLFLAVSIMDDSSS 291

BLAST of Chy12G210300 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-79
Identity = 144/288 (50.00%), Postives = 205/288 (71.18%), Query Frame = 0

Query: 8   IHLSLIAFIVICS------SFSLSNAASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSS 67
           ++ S  +F  +C       SF++   + ++  E FL+C   + P++ +  K IYTQ++  
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFNI-QISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQL 60

Query: 68  YSSVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGL 127
           Y SVLN +I+N RF++  TPKPL+I+TPSN+SHIQA+++CSK  GLQIR RSGGHD EGL
Sbjct: 61  YMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGL 120

Query: 128 SYVAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTV 187
           SY++ +PF +VDL N+ ++ V++  +TAWV++ ATLGE+YY I E +   +FPGG CPTV
Sbjct: 121 SYISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTV 180

Query: 188 CFGGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFG 247
             GG+ SGGGYG L+R YG+AADN+IDA+LV+  G+  DR+SMGEDLFWAIRGGGG +FG
Sbjct: 181 GVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFG 240

Query: 248 IVVAWKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLENNI 289
           I+ AWK+KLV VP+  T  S  +  E    + L ++WQ + YK + ++
Sbjct: 241 IIAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDL 287

BLAST of Chy12G210300 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.6e-119
Identity = 218/281 (77.58%), Postives = 242/281 (86.12%), Query Frame = 0

Query: 13  IAFIVICSSFSLSNAASSD-KHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIR 72
           +AFIV+CSS S+  +ASS   HEEFL CL+HHSP S SITKL+YT  N+SYS VLN SIR
Sbjct: 11  LAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIR 70

Query: 73  NHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFSTPNTPKPLLIITPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+V
Sbjct: 71  NLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVV 130

Query: 133 VDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLINL S+T+ VE+ TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG
Sbjct: 131 VDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYG+AADNVIDAY+VDA GEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGV 293
           PVPATVT C+ SRT EE+AI LI QWQYV  KL+ ++  G+
Sbjct: 251 PVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGI 291

BLAST of Chy12G210300 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.6e-119
Identity = 218/281 (77.58%), Postives = 242/281 (86.12%), Query Frame = 0

Query: 13  IAFIVICSSFSLSNAASSD-KHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIR 72
           +AFIV+CSS S+  +ASS   HEEFL CL+HHSP S SITKL+YT  N+SYS VLN SIR
Sbjct: 11  LAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIR 70

Query: 73  NHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFSTPNTPKPLLIITPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+V
Sbjct: 71  NLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVV 130

Query: 133 VDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLINL S+T+ VE+ TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG
Sbjct: 131 VDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYG+AADNVIDAY+VDA GEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGV 293
           PVPATVT C+ SRT EE+AI LI QWQYV  KL+ ++  G+
Sbjct: 251 PVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGI 291

BLAST of Chy12G210300 vs. ExPASy TrEMBL
Match: A0A5D3C1K3 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00270 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 4.9e-118
Identity = 221/290 (76.21%), Postives = 246/290 (84.83%), Query Frame = 0

Query: 5   SPSIHLSLIAFIVICSSFSLSNAASSD-KHEEFLQCLIHHSPHSSSITKLIYTQNNSSYS 64
           SP + LSL+ FIV+CSS SL  A SS  KHEEFLQCL+HHSP +SSI KL++T  NSS+S
Sbjct: 4   SPLVPLSLV-FIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFS 63

Query: 65  SVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSY 124
           ++ N   RN RFSTPNTPKPLLIITPSNISHIQAAVICSKS GLQIRIRSGGHDFEGLSY
Sbjct: 64  TLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 123

Query: 125 VAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCF 184
           VAY  FIVVDLINLRS+TV+VE+ TAWVQS ATLGELYY+I +KS TL FP G CPTV  
Sbjct: 124 VAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGV 183

Query: 185 GGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIV 244
           GG+ SGGGYG LLRKYGVAADNVIDAYLVDA GEFHDRESMGEDLFWAIRGGGGGSFG+V
Sbjct: 184 GGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVV 243

Query: 245 VAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVL 294
           VAWKVKLVPVPA+VT CS+ R+ EE+AI LI +WQYV  KLE ++   +L
Sbjct: 244 VAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAIL 292

BLAST of Chy12G210300 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 4.9e-118
Identity = 221/290 (76.21%), Postives = 246/290 (84.83%), Query Frame = 0

Query: 5   SPSIHLSLIAFIVICSSFSLSNAASSD-KHEEFLQCLIHHSPHSSSITKLIYTQNNSSYS 64
           SP + LSL+ FIV+CSS SL  A SS  KHEEFLQCL+HHSP +SSI KL++T  NSS+S
Sbjct: 17  SPLVPLSLV-FIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFS 76

Query: 65  SVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSY 124
           ++ N   RN RFSTPNTPKPLLIITPSNISHIQAAVICSKS GLQIRIRSGGHDFEGLSY
Sbjct: 77  TLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 136

Query: 125 VAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCF 184
           VAY  FIVVDLINLRS+TV+VE+ TAWVQS ATLGELYY+I +KS TL FP G CPTV  
Sbjct: 137 VAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGV 196

Query: 185 GGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIV 244
           GG+ SGGGYG LLRKYGVAADNVIDAYLVDA GEFHDRESMGEDLFWAIRGGGGGSFG+V
Sbjct: 197 GGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVV 256

Query: 245 VAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVL 294
           VAWKVKLVPVPA+VT CS+ R+ EE+AI LI +WQYV  KLE ++   +L
Sbjct: 257 VAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAIL 305

BLAST of Chy12G210300 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 7.8e-116
Identity = 214/291 (73.54%), Postives = 245/291 (84.19%), Query Frame = 0

Query: 3   KSSPSIHLSLIAFIVICSSFSLSNAASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSY 62
           K SP   LSL AFI++ SS S + AAS++KHE FLQCL HHS  SSSI+K+IYT  N SY
Sbjct: 2   KYSPLTILSL-AFILLSSSSSWA-AASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSY 61

Query: 63  SSVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 122
           SSVLN S+RN RFS+  TPKPLLIITPS +SH+QAAVICSK+ GLQIRIRSGGHD EGLS
Sbjct: 62  SSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLS 121

Query: 123 YVAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 182
           YVA+LPFI++DLINL+SITVN+E  TAW+QS ATLGELYY IA+KS TL FP G CPTV 
Sbjct: 122 YVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVG 181

Query: 183 FGGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGI 242
            GG+ SGGGYGL+LRKYG+AADNVIDAYL+DA G+F+DRESMGEDLFWAIRGGGGGSFGI
Sbjct: 182 VGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGI 241

Query: 243 VVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVL 294
           VVAWKVKLVPVPA VT CSS R  +ED INLIH+WQ V YKL+ N+  G++
Sbjct: 242 VVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGII 290

BLAST of Chy12G210300 vs. NCBI nr
Match: XP_004135739.1 (berberine bridge enzyme-like 28 [Cucumis sativus])

HSP 1 Score: 574 bits (1479), Expect = 7.98e-202
Identity = 286/303 (94.39%), Postives = 294/303 (97.03%), Query Frame = 0

Query: 1   MKKSSPSIHLSLIAFIVICSSFSLSNAASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNS 60
           MKKSSPSIHLSLIAFIVICSSFSL NAASSDKHEEFLQCL+HHSPHSSSI KLIYTQNNS
Sbjct: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS 60

Query: 61  SYSSVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120
           SYSSVLNLSIRNHRFSTPNTPKP+LIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG
Sbjct: 61  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 180
           LSYVAYLPFIVVDLINLRSITV+V+RRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT
Sbjct: 121 LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 180

Query: 181 VCFGGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSF 240
           VCFGGYLSGGGYGLLLRKYG+AADNVIDAYLVDA GEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVLTIDNESN 300
           GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLE NIAHGVLTI  +  
Sbjct: 241 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT 300

Query: 301 NRI 303
           + +
Sbjct: 301 SEV 303

BLAST of Chy12G210300 vs. NCBI nr
Match: KAE8653320.1 (hypothetical protein Csa_023331 [Cucumis sativus])

HSP 1 Score: 526 bits (1356), Expect = 1.69e-183
Identity = 260/276 (94.20%), Postives = 268/276 (97.10%), Query Frame = 0

Query: 28  ASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPLLII 87
           ASSDKHEEFLQCL+HHSPHSSSI KLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKP+LII
Sbjct: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63

Query: 88  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVNVERR 147
           TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITV+V+RR
Sbjct: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123

Query: 148 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGVAADNVI 207
           TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYG+AADNVI
Sbjct: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183

Query: 208 DAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 267
           DAYLVDA GEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE
Sbjct: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243

Query: 268 EDAINLIHQWQYVGYKLENNIAHGVLTIDNESNNRI 303
           EDAINLIHQWQYVGYKLE NIAHGVLTI  +  + +
Sbjct: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEV 279

BLAST of Chy12G210300 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 441 bits (1133), Expect = 1.61e-149
Identity = 223/288 (77.43%), Postives = 247/288 (85.76%), Query Frame = 0

Query: 3   KSSPSIHLSLIAFIVICSSFSLSNAASSD--KHEEFLQCLIHHSPHSSSITKLIYTQNNS 62
           K SP I LSL AFI++CSS SL +A SS   KHEEFLQCL+HHSP++ SITKL+Y   NS
Sbjct: 2   KFSPLILLSL-AFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 61

Query: 63  SYSSVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 122
           SYSS+LN   RN RFSTPN P PLLIITP+NISHIQAA+ICSKS GLQIRIRSGGHDFEG
Sbjct: 62  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 121

Query: 123 LSYVAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 182
           LSYVAY+PFIVVDLINLRS+TV+ E+ +AWVQS ATLGELYYRIAEKS TL FP G CPT
Sbjct: 122 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 181

Query: 183 VCFGGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSF 242
           V  GG+ SGGGYG+LLRKYG+AADNVIDAYLVDA GEFHDRESMGEDLFWAIRGGGGG+F
Sbjct: 182 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 241

Query: 243 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNI 288
           G+VVAWKVKLVPVP TVT C++ RT EE AI LI QWQYVG KLE ++
Sbjct: 242 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDL 288

BLAST of Chy12G210300 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 437 bits (1123), Expect = 5.08e-148
Identity = 224/293 (76.45%), Postives = 249/293 (84.98%), Query Frame = 0

Query: 3   KSSPSIHLSLIAFIVICSSFSLSNAASSD--KHEEFLQCLIHHSPHSSSITKLIYTQNNS 62
           K SP I LSL AFI++CSS SL +A SS   K+EEFLQCL+HHSP++ SIT L+Y   NS
Sbjct: 2   KFSPLIPLSL-AFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 61

Query: 63  SYSSVLNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 122
           SYSS+LN SIRN RFSTP  PKPLLIITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEG
Sbjct: 62  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 121

Query: 123 LSYVAYLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 182
           LSYVAY PFIVVDLINL S+ V+VE+ TAWVQS ATLGELYYRIAEKS TL FP G CPT
Sbjct: 122 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 181

Query: 183 VCFGGYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSF 242
           V  GG+ SGGGYG LLRKYG+AADNVIDAYLVDA GEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 182 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 241

Query: 243 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVL 293
           GIVVAWKVKLVPVPATVT C+++RT++E AI LI QWQYV  KL+ ++   ++
Sbjct: 242 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSII 293

BLAST of Chy12G210300 vs. NCBI nr
Match: XP_008445709.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo] >KAA0047097.1 cannabidiolic acid synthase-like [Cucumis melo var. makuwa] >TYK05061.1 cannabidiolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 435 bits (1118), Expect = 2.81e-147
Identity = 218/281 (77.58%), Postives = 242/281 (86.12%), Query Frame = 0

Query: 13  IAFIVICSSFSLSNAASSD-KHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIR 72
           +AFIV+CSS S+  +ASS   HEEFL CL+HHSP S SITKL+YT  N+SYS VLN SIR
Sbjct: 11  LAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIR 70

Query: 73  NHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFSTPNTPKPLLIITPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+V
Sbjct: 71  NLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVV 130

Query: 133 VDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLINL S+T+ VE+ TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG
Sbjct: 131 VDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYG+AADNVIDAY+VDA GEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGV 292
           PVPATVT C+ SRT EE+AI LI QWQYV  KL+ ++  G+
Sbjct: 251 PVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGI 291

BLAST of Chy12G210300 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 298.9 bits (764), Expect = 4.7e-81
Identity = 141/262 (53.82%), Postives = 196/262 (74.81%), Query Frame = 0

Query: 27  AASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPLLI 86
           +A+      FLQCL      S+ ++K+I+T N++S+SSVL  SI+N RFS P+ PKP+LI
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 87

Query: 87  ITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVNVER 146
           +TP   S +Q+AV C++  G+ IR RSGGHD+EGLSYV + PF+++DL NLRSITV+V+ 
Sbjct: 88  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 147

Query: 147 RTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGVAADNV 206
           R+ WVQ+ AT+GELYY I +K+ TL FP G CPTV  GG+ SGGGYG LLRK+G+AAD+V
Sbjct: 148 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 207

Query: 207 IDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTF 266
           IDA +VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT  + ++  
Sbjct: 208 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 267

Query: 267 EEDAINLIHQWQYVGYKLENNI 289
           E+ A+ +IH+WQ+V  K+ +++
Sbjct: 268 EQSALKIIHRWQFVADKVSDDL 289

BLAST of Chy12G210300 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 295.0 bits (754), Expect = 6.8e-80
Identity = 148/281 (52.67%), Postives = 196/281 (69.75%), Query Frame = 0

Query: 10  LSLIAFIVICSSFSLSNAASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSVLNLS 69
           L L+ F V+   F   +++SS   E F QCL  +S     I+  I+   N SYSSVL  +
Sbjct: 5   LILVLFFVV---FIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQAN 64

Query: 70  IRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAY--L 129
           IRN RF+T +TPKP LII  ++ SH+QAA+ C K   LQ++IRSGGHD++GLSYV Y   
Sbjct: 65  IRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGK 124

Query: 130 PFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYL 189
           PF V+D+ NLRS+ V+V  +TAWVQ+ A LGE+YY I EKS TL +P G CPTV  GG++
Sbjct: 125 PFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHI 184

Query: 190 SGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWK 249
           SGGGYG ++RKYG+  DN IDA +VD  G+  DR+ MGEDL+WAI GGGGGS+G+V+A+K
Sbjct: 185 SGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYK 244

Query: 250 VKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNI 289
           + LV VP  VT    SRT E++A ++IH+WQ V  KL + +
Sbjct: 245 INLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDEL 282

BLAST of Chy12G210300 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 288.9 bits (738), Expect = 4.9e-78
Identity = 144/305 (47.21%), Postives = 207/305 (67.87%), Query Frame = 0

Query: 8   IHLSLIAFIVICSSFSLSN--AASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSV 67
           +  S   F ++  S ++S   +A    HE+FL+CL +    ++   K+I+T  +SS+ S+
Sbjct: 1   MEFSSFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSI 60

Query: 68  LNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 127
           L+ SI+N RFS   TPKP+ IITP   S +Q  + C++  G+ +R RS GH +EGLSY+A
Sbjct: 61  LDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIA 120

Query: 128 Y-LPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFG 187
           Y  PF V+DL NLRSI+++V+ RT WVQ+ AT GELYY I + + +L FP G  PTV  G
Sbjct: 121 YNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVG 180

Query: 188 GYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVV 247
           G  SGGGYG LLRKYG+AADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG+++
Sbjct: 181 GQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVIL 240

Query: 248 AWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNIAHGVLTIDNESNNRIL 307
           +WKVKLV VP+T+T     +T +++A+ +I +WQY   K+ +++     T++  + N + 
Sbjct: 241 SWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRT-TLERSNKNAVH 300

Query: 308 REFTG 310
             FTG
Sbjct: 301 ALFTG 304

BLAST of Chy12G210300 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 288.1 bits (736), Expect = 8.3e-78
Identity = 144/284 (50.70%), Postives = 200/284 (70.42%), Query Frame = 0

Query: 10  LSLIAFIVICSSFSLSN----AASSDKHEEFLQCLIHHSPHSSSITKLIYTQNNSSYSSV 69
           LSL  + ++  + SLS+    +A    HE FL+CL H      S  ++I+T  + SY S+
Sbjct: 8   LSLFIYFLLL-NLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSI 67

Query: 70  LNLSIRNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 129
           LN SI+N RF    TPKP+ IITP   + +Q+ + C++  G+ IR RSGGHD+EGLSY+A
Sbjct: 68  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 127

Query: 130 -YLPFIVVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFG 189
              PF+V+DL NLRSIT++V+ RT WVQS AT+GELYY I + S +L FP G  PTV  G
Sbjct: 128 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 187

Query: 190 GYLSGGGYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVV 249
           G   GGGYG L+RKYG++ADNVIDA++VDA G F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 188 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 247

Query: 250 AWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNI 289
           +WK++L+ VP+ VT     +T E++A+++I++WQY+  K+ N++
Sbjct: 248 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDL 288

BLAST of Chy12G210300 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.4e-77
Identity = 139/278 (50.00%), Postives = 196/278 (70.50%), Query Frame = 0

Query: 13  IAFIVICSSFSLSNAASSDK-HEEFLQCLIHHSPH-SSSITKLIYTQNNSSYSSVLNLSI 72
           + F+V    FS +   SSD  +E F+QC    +    + IT  ++++ N S+SSVL   I
Sbjct: 14  VFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYI 73

Query: 73  RNHRFSTPNTPKPLLIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFI 132
           RN RF+T +TPKP +I+TP +  H+ AAV CSKS    ++IRSGGHD+EGLSY++  PF 
Sbjct: 74  RNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFF 133

Query: 133 VVDLINLRSITVNVERRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGG 192
           ++D+ NLR ++V++  ++AW+ + ATLGE+YYRI EKS    FP G CPTV  GG++SGG
Sbjct: 134 ILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGG 193

Query: 193 GYGLLLRKYGVAADNVIDAYLVDATGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKL 252
           GYG +LRK+G++ DN+IDA +VD  G+  DR+SMGEDLFWAI GGGG SFG+V+ +KVKL
Sbjct: 194 GYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKL 253

Query: 253 VPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLENNI 289
           VPVP TVT     +  +  A++++H+WQ VG K + N+
Sbjct: 254 VPVPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNL 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GTB61.2e-8155.00Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... [more]
A6P6V92.3e-8049.65Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1[more]
Q9SVG56.6e-8053.82Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
A0A2S1XB678.7e-8052.56O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1[more]
Q33DQ21.1e-7950.00Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TYU22.6e-11977.58Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BCV72.6e-11977.58cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV... [more]
A0A5D3C1K34.9e-11876.21Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BDD94.9e-11876.21tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348866... [more]
A0A6J1DF277.8e-11673.54berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE... [more]
Match NameE-valueIdentityDescription
XP_004135739.17.98e-20294.39berberine bridge enzyme-like 28 [Cucumis sativus][more]
KAE8653320.11.69e-18394.20hypothetical protein Csa_023331 [Cucumis sativus][more]
XP_038895262.11.61e-14977.43tetrahydrocannabinolic acid synthase-like [Benincasa hispida][more]
XP_038895343.15.08e-14876.45cannabidiolic acid synthase-like [Benincasa hispida][more]
XP_008445709.12.81e-14777.58PREDICTED: cannabidiolic acid synthase-like [Cucumis melo] >KAA0047097.1 cannabi... [more]
Match NameE-valueIdentityDescription
AT4G20820.14.7e-8153.82FAD-binding Berberine family protein [more]
AT1G30700.16.8e-8052.67FAD-binding Berberine family protein [more]
AT5G44440.14.9e-7847.21FAD-binding Berberine family protein [more]
AT5G44410.18.3e-7850.70FAD-binding Berberine family protein [more]
AT4G20840.12.4e-7750.00FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 83..219
e-value: 2.4E-25
score: 88.9
NoneNo IPR availableGENE3D3.40.462.20coord: 256..303
e-value: 1.9E-68
score: 233.6
NoneNo IPR availablePANTHERPTHR32448:SF76CANNABIDIOLIC ACID SYNTHASE-LIKE 1coord: 13..292
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 13..292
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 30..137
e-value: 6.6E-45
score: 153.3
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 142..255
e-value: 1.9E-68
score: 233.6
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 79..253
score: 19.067053
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 32..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G210300.1Chy12G210300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding