Chy12G210280 (gene) Cucumber (hystrix) v1

BLAST of Chy12G210280 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.1e-149
Identity = 263/538 (48.88%), Postives = 362/538 (67.29%), Query Frame = 0

Query: 17  CSSSSLW-------------VAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSSFSS 76
           CS+ S W             +  S +   E F++C   H P+     KLV+T  +  + S
Sbjct: 3   CSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMS 62

Query: 77  LLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLSYV 136
           +LN   +N RF +  TPKPL+I+TPSN SHIQA ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  ILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYI 122

Query: 137 SYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           S   F+VVDL N+ S+ +DV   TAWV++GATLGE+YY I +K+  L FP G CPTVGVG
Sbjct: 123 SQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHFSGGGYG+L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ 
Sbjct: 183 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSAQE 316
           AWK+KLV VP+  TI S K+++E    +KL ++WQ +A K ++DL L   +    ++   
Sbjct: 243 AWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNH 302

Query: 317 G-GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G  K      F ++F G V+ L+ ++NK+FPELG+ K +CKE SWI++    S    F  
Sbjct: 303 GKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 436
            +   E LL+R+     +F +K DYVK+P+ + A+  I  +L  +D+      + PYGG 
Sbjct: 363 ANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGI 422

Query: 437 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 496
           M +ISES IPF HRAGI+Y++ Y   WE++  E  ++H+NW+R +Y++  P+VS++PR A
Sbjct: 423 MEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIP 538
           Y+NYRDLD+G  N     +Y QAR++G KYFG NFNRLVKVK+KVDP NFF +EQSIP
Sbjct: 483 YLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of Chy12G210280 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 263/544 (48.35%), Postives = 370/544 (68.01%), Query Frame = 0

Query: 17  CSSSSLW-------------VAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSSFSS 76
           CS+ S W             +  S +   E F++C   + P+     KLV+T  N  + S
Sbjct: 3   CSTFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMS 62

Query: 77  LLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLSYV 136
           +LN    N RF++  TPKPL+I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 122

Query: 137 SYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           S   F++VDL N+RS+ +DV   TAWV++GATLGE+YY + +K+  L   AG CPTV  G
Sbjct: 123 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHF GGGYG L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+V
Sbjct: 183 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSAQE 316
           AWK++LV VP + T+ S K+ +E  + +KL+++WQ +A K ++DL L   +    ++  +
Sbjct: 243 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 302

Query: 317 GGKINPIALFF-TLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G     I  +F ++FLG V+ L+ ++NK+FPELG+ K +C++ SWI++    S    +  
Sbjct: 303 GKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 436
           ++   E LL+R+   +G+FK+K DYVK+P+ ++    I  +L  +DI     A+ PYGG 
Sbjct: 363 DNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGI 422

Query: 437 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 496
           M +ISES IPF HRAGILY++ YI  WE++  E  ++HLNWIR IY++M P+VSK+PR A
Sbjct: 423 MDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLS 544
           Y+NYRDLDIG N+     +Y QAR++G KYFG NF+RLVKVK+ VDP NFF +EQSIP  
Sbjct: 483 YLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 542

BLAST of Chy12G210280 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.0e-147
Identity = 258/515 (50.10%), Postives = 357/515 (69.32%), Query Frame = 0

Query: 33  EEFIQCLLHHSPDTYFITKLVHTPINSSFSSLLNFPSRNSRFSTPNTPKPLLIITPSNIS 92
           E F++CL  + P      KLV+T  +  + S+LN   +N RF++  TPKPL+I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HIQAAVVCSKSQGLQIRIRSGGHDFEGLSYVSYHQFIVVDLINLRSVTVDVEQSTAWVQS 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY+S   F++VDL N+ SV +DV   TAWV+S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEDLFLAILWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E++L L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +   +   E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMSQISESKIPFSHRAGILYQIGYILGWEE 452
           + + A+  I  +L  +D+        PYGG M +ISES IPF HRAGI Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMAPFVSKSPRAAYVNYRDLDIGSNNKYGKIDYKQARVFGSK 512
           +  E  ++H+NWIR +Y++  P+VS++PR AY+NYRDLD+G  N     +Y QAR++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSKVDPYNFFWHEQSI-PLSLNH 543
           YFG NFNRLVKVK+KVDP NFF +EQSI PL L H
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPLRH 544

BLAST of Chy12G210280 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.2e-147
Identity = 257/515 (49.90%), Postives = 357/515 (69.32%), Query Frame = 0

Query: 33  EEFIQCLLHHSPDTYFITKLVHTPINSSFSSLLNFPSRNSRFSTPNTPKPLLIITPSNIS 92
           E F++C   + P      KLV+T  +  + S+LN   +N RF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQAAVVCSKSQGLQIRIRSGGHDFEGLSYVSYHQFIVVDLINLRSVTVDVEQSTAWVQS 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY+S   F++VDL N+ SV +DV   TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEDLFLAILWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E++L L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +       E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMSQISESKIPFSHRAGILYQIGYILGWEE 452
           + + A+  I  +L  +D+        PYGG M +ISES IPF HRAGI+Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMAPFVSKSPRAAYVNYRDLDIGSNNKYGKIDYKQARVFGSK 512
           +  E  ++H+NWIR +Y++  P+VS++PR AY+NYRDLD+G  N     +Y QAR++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSKVDPYNFFWHEQSI-PLSLNH 543
           YFG NFNRLVKVK+KVDP NFF +EQSI PL L H
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPLRH 544

BLAST of Chy12G210280 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.5e-146
Identity = 253/514 (49.22%), Postives = 363/514 (70.62%), Query Frame = 0

Query: 24  VAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSSFSSLLNFPSRNSRFSTPNTPKPL 83
           V+A+ S N   F+QCL     D+  ++K++HTP ++SFSS+L    +N RFS P+ PKP+
Sbjct: 27  VSANRS-NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPV 86

Query: 84  LIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLSYVSYHQFIVVDLINLRSVTVDV 143
           LI+TP   S +Q+AV C++  G+ IR RSGGHD+EGLSYV++  F+++DL NLRS+TVDV
Sbjct: 87  LILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDV 146

Query: 144 EQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAAD 203
           +  + WVQ+GAT+GELYY+IGKK+RTL FPAG+CPTVGVGGHFSGGGYG+LLRK+G+AAD
Sbjct: 147 DNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAAD 206

Query: 204 NVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKR 263
           +VIDA +VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT+ +  +
Sbjct: 207 HVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTK 266

Query: 264 SLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSAQEGGKINPIALFFTLFLGNVNEL 323
             E+ A+K+I +WQ+VA+K+ +DLF+ ++    K   +        A F  L+LG+V  L
Sbjct: 267 FSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVR--------ASFPGLYLGSVKNL 326

Query: 324 MAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPITHGSFKLKSD 383
           + ++NK FPELGL +++C E SWIES    A   ++ ++P+  L  RT  +  +FK KSD
Sbjct: 327 LKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTRASL-AFKAKSD 386

Query: 384 YVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMSQISESKIPFSHRAGILYQIGYI 443
           +V+EPM K AI  +WRRL+  + E   L   P+GG+MS+I++ + PF HR G +Y+I Y+
Sbjct: 387 FVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYL 446

Query: 444 LGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAYVNYRDLDIGSNNKYGKIDYKQAR 503
             W     + +++++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      +  Y++ +
Sbjct: 447 NYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGK 506

Query: 504 VFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIP 538
            +G KYF NNF RLV+VK+ VDP +FF  EQSIP
Sbjct: 507 SWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210280 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.5e-295
Identity = 502/542 (92.62%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS 60
           M LSPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  FSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGL 120
           FS+L NFP+RNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYV+YH+FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEEDLFLAILWNGGKVSA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AIEGIW+RLESQDIEGVTLA+IPYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 480
           MS+ISES IPFSHRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYM PFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNKYGKI+YKQA VFGSKYFGNNFNRLVKVK+KVDPYNFFWHEQSIPLS
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

Query: 541 LN 542
            N
Sbjct: 554 SN 555

BLAST of Chy12G210280 vs. ExPASy TrEMBL
Match: A0A5D3C1K3 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00270 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.7e-278
Identity = 481/542 (88.75%), Postives = 500/542 (92.25%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS 60
           M LSPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 61  FSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGL 120
           FS+L NFP+RNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYV+YH+FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEEDLFLAILWNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AIEGIW+RLESQDIEGVTLA+IPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 421 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 480
           MS+ISES IPFSHRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYM PFVSKSPR A
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 481 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNK                       LVKVK+KVDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 519

Query: 541 LN 542
            N
Sbjct: 541 SN 519

BLAST of Chy12G210280 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.7e-252
Identity = 425/544 (78.12%), Postives = 483/544 (88.79%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS 60
           MK S L+PLSL F+VVCSSSS+W +ASS +NHEEF+ CLLHHSPD+  ITKLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  FSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGL 120
           +S +LNF  RN RFSTPNTPKPLLIITPSNISH+QAAV+CSKS G+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYV+YH F+VVDLINL SVT++VEQSTAWVQSGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKLIDQWQYVANKL+EDLFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+ +G+ K+KSDYVKEP++KA I+ +W+RL+SQDIEG  L  IPYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 SQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAY 480
           SQISES+IPFSHRAG LY+IGY+ GW E G+ AEK HLNWIR+IYSYM PFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLSL 540
           VNYRDLDIGSN+KYGK  YK+A V+GSKYFG+NF+RLV VK++VDPYNFF HEQSIP+ L
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLL 540

Query: 541 NHRL 545
             R+
Sbjct: 541 KRRI 543

BLAST of Chy12G210280 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.7e-252
Identity = 425/544 (78.12%), Postives = 483/544 (88.79%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS 60
           MK S L+PLSL F+VVCSSSS+W +ASS +NHEEF+ CLLHHSPD+  ITKLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  FSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGL 120
           +S +LNF  RN RFSTPNTPKPLLIITPSNISH+QAAV+CSKS G+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYV+YH F+VVDLINL SVT++VEQSTAWVQSGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKLIDQWQYVANKL+EDLFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+ +G+ K+KSDYVKEP++KA I+ +W+RL+SQDIEG  L  IPYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 SQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAY 480
           SQISES+IPFSHRAG LY+IGY+ GW E G+ AEK HLNWIR+IYSYM PFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLSL 540
           VNYRDLDIGSN+KYGK  YK+A V+GSKYFG+NF+RLV VK++VDPYNFF HEQSIP+ L
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLL 540

Query: 541 NHRL 545
             R+
Sbjct: 541 KRRI 543

BLAST of Chy12G210280 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 7.8e-228
Identity = 388/536 (72.39%), Postives = 457/536 (85.26%), Query Frame = 0

Query: 4   SPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPD--TYFITKLVHTPINSSF 63
           SPLI LSL F+ + SSSSLW +A  +  HE F+QCLL HS +   Y I+K++HTPINSS+
Sbjct: 5   SPLISLSLAFIFL-SSSSLWASAFPN-KHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 64

Query: 64  SSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLS 123
           SS+L+F  RN RFST  TPKPLLI+TPS++SHIQAAV+CSKS G QIR RSGGHDFEGLS
Sbjct: 65  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 124

Query: 124 YVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVG 183
           YV++  FI+VDLINLRS+++D++ +TAWVQSGATLGELYY I +KSRTL FPAGICPTVG
Sbjct: 125 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 184

Query: 184 VGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 243
           VGGHFSGGGYG LLRKYG+AADNVIDAYLVDA+G+FH+RESMGEDLFWAIRGGGGGSFGI
Sbjct: 185 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 244

Query: 244 VVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSAQ 303
           VVAWKVKLVPVPATVT+CSA R+LEE A+K+  +WQYVANKL+E+LFL ILW GGK+++Q
Sbjct: 245 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 304

Query: 304 EGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIED 363
           EGGK NP+A FF+LFLG  +EL++ILN  FPEL L K++C E SWIES       F  E 
Sbjct: 305 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTE- 364

Query: 364 QPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMS 423
             LEALL+RTP+T+ S K+KSDYVKEPM++A ++GIW+RL+SQDIEG  +  +PYGGRMS
Sbjct: 365 LTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMS 424

Query: 424 QISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAYV 483
           QISES+ PFSHRAG LY+IGYI GWEE+G++AEKRHL WIRE+Y YM PFVSKSPRAAY 
Sbjct: 425 QISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYA 484

Query: 484 NYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIP 538
           NYRDLDIGSNNK+GK  YKQA V+G KYFGNNFNRLV VK+KVDPY+FF HEQSIP
Sbjct: 485 NYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 537

BLAST of Chy12G210280 vs. NCBI nr
Match: XP_011659211.2 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653319.1 hypothetical protein Csa_023346 [Cucumis sativus])

HSP 1 Score: 1056 bits (2731), Expect = 0.0
Identity = 522/542 (96.31%), Postives = 533/542 (98.34%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS 60
           MK SPLIPLSLVFVVVCSSSSLW+AASSSINHEEFIQCLLHHSPDTY ITKLVHTPINSS
Sbjct: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60

Query: 61  FSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGL 120
           FSSLLNFP+RNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKS GLQIRIRSGGHDFEGL
Sbjct: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYV+YHQFIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSA 300
           IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRM 420
           DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAI+GIW+RLESQDIEGVTLAVIPYGGRM
Sbjct: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420

Query: 421 SQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAY 480
           +QISESKIPF HRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYM PFVSKSPRAAY
Sbjct: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLSL 540
           VNYRDLDIGSNNKYGKI+YKQA VFGSKYFGNNFNRLVKVKS VDPYNFFWHEQSIPLS 
Sbjct: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540

Query: 541 NH 542
           +H
Sbjct: 541 DH 542

BLAST of Chy12G210280 vs. NCBI nr
Match: XP_008445721.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1018 bits (2632), Expect = 0.0
Identity = 502/542 (92.62%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS 60
           M LSPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  FSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGL 120
           FS+L NFP+RNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYV+YH+FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEEDLFLAILWNGGKVSA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEALLNRTPITHGS-FKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HGS FKLKSDYVKEPMTK AIEGIW+RLESQDIEGVTLA+IPYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 480
           MS+ISES IPFSHRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYM PFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNKYGKI+YKQA VFGSKYFGNNFNRLVKVK+KVDPYNFFWHEQSIPLS
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

BLAST of Chy12G210280 vs. NCBI nr
Match: KAA0047096.1 (tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05062.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 481/542 (88.75%), Postives = 500/542 (92.25%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS 60
           M LSPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 61  FSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGL 120
           FS+L NFP+RNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYV+YH+FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEEDLFLAILWNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHGS-FKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HGS FKLKSDYVKEPMTK AIEGIW+RLESQDIEGVTLA+IPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 421 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 480
           MS+ISES IPFSHRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYM PFVSKSPR A
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 481 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNK                       LVKVK+KVDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 519

BLAST of Chy12G210280 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 914 bits (2363), Expect = 0.0
Identity = 448/545 (82.20%), Postives = 497/545 (91.19%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSS-INHEEFIQCLLHHSPDTYFITKLVHTPINS 60
           MK SPLI LSL F++VCSSSSL  A SSS I HEEF+QCLLHHSP+TY ITKLV+ PINS
Sbjct: 1   MKFSPLILLSLAFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 60

Query: 61  SFSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEG 120
           S+SSLLNFPSRNSRFSTPN P PLLIITP+NISHIQAA++CSKS GLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPT 180
           LSYV+Y  FIVVDLINLRSVTVD E+S+AWVQSGATLGELYY+I +KS+TL FPAG+CPT
Sbjct: 121 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG+F
Sbjct: 181 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 240

Query: 241 GIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVS 300
           G+VVAWKVKLVPVP TVTIC+AKR+LEE AI+LIDQWQYV NKLEEDLFL+I  NGGK S
Sbjct: 241 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNS 300

Query: 301 AQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQI 360
           AQ GGK+NP ALFF+LFLG  +EL+AIL K FPELGLTKE+CKEKSWI SA SAANRFQI
Sbjct: 301 AQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQI 360

Query: 361 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 420
           EDQPLEALLNRTP+ +GS K+KSDYVKE +TK  IEGIW RL+SQDIEGVTLA+IPYGGR
Sbjct: 361 EDQPLEALLNRTPLANGSLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYGGR 420

Query: 421 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 480
           M QIS+S+IPFSHRAGI YQIGYI+GW+E+G++AEKRH+NWIREIYSYM PFVSKSPRAA
Sbjct: 421 MGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLS 540
           YVNYRD DIGSNNKYGK+ YK+ARV+G KYFGNNFNRLV++K+KVDP NFF HEQSIPLS
Sbjct: 481 YVNYRDHDIGSNNKYGKVSYKRARVWGLKYFGNNFNRLVRLKTKVDPSNFFRHEQSIPLS 540

Query: 541 LNHRL 544
            NHR+
Sbjct: 541 PNHRI 545

BLAST of Chy12G210280 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 439/544 (80.70%), Postives = 483/544 (88.79%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSS-INHEEFIQCLLHHSPDTYFITKLVHTPINS 60
           MK SPLIPLSL F+++CSSSSL  A SSS I +EEF+QCLLHHSP+TY IT LV+ P NS
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 61  SFSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEG 120
           S+SSLLNF  RN RFSTP  PKPLLIITPSNISHIQAAVVCSKS GLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVSYHQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPT 180
           LSYV+YH FIVVDLINL SV VDVEQSTAWVQSGATLGELYY+I +KSRTL FPAG CPT
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 181 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG+LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVS 300
           GIVVAWKVKLVPVPATVTIC+A R+ +E AIKLIDQWQYVANKL+EDLFL+I+  GGK+S
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 301 AQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQI 360
           AQ GGKINP ALFFTLFLG  +EL+ IL   FPEL L KE+CKE SWIESA   AN  QI
Sbjct: 301 AQ-GGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 360

Query: 361 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGR 420
           +DQPLEA LNRTP+ +GSFK+KSDYVKEP+TKAAIEGIW+RL+SQDIEGV L +IPYGGR
Sbjct: 361 DDQPLEAFLNRTPLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGGR 420

Query: 421 MSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 480
           MSQISES+IPF HR G LY+IGYI+GWEE+GV+AEKRHLNWIREIYSYM PFVSKSPRAA
Sbjct: 421 MSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSN+KYGK  YK+A ++G KYFGNNFNRLV VK++VDPY+FF HEQSIP  
Sbjct: 481 YVNYRDLDIGSNDKYGKTSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFRHEQSIPSF 540

Query: 541 LNHR 543
            +HR
Sbjct: 541 PHHR 543

BLAST of Chy12G210280 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.8e-147
Identity = 253/514 (49.22%), Postives = 363/514 (70.62%), Query Frame = 0

Query: 24  VAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSSFSSLLNFPSRNSRFSTPNTPKPL 83
           V+A+ S N   F+QCL     D+  ++K++HTP ++SFSS+L    +N RFS P+ PKP+
Sbjct: 27  VSANRS-NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPV 86

Query: 84  LIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLSYVSYHQFIVVDLINLRSVTVDV 143
           LI+TP   S +Q+AV C++  G+ IR RSGGHD+EGLSYV++  F+++DL NLRS+TVDV
Sbjct: 87  LILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDV 146

Query: 144 EQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAAD 203
           +  + WVQ+GAT+GELYY+IGKK+RTL FPAG+CPTVGVGGHFSGGGYG+LLRK+G+AAD
Sbjct: 147 DNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAAD 206

Query: 204 NVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKR 263
           +VIDA +VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT+ +  +
Sbjct: 207 HVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTK 266

Query: 264 SLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSAQEGGKINPIALFFTLFLGNVNEL 323
             E+ A+K+I +WQ+VA+K+ +DLF+ ++    K   +        A F  L+LG+V  L
Sbjct: 267 FSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVR--------ASFPGLYLGSVKNL 326

Query: 324 MAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPITHGSFKLKSD 383
           + ++NK FPELGL +++C E SWIES    A   ++ ++P+  L  RT  +  +FK KSD
Sbjct: 327 LKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTRASL-AFKAKSD 386

Query: 384 YVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMSQISESKIPFSHRAGILYQIGYI 443
           +V+EPM K AI  +WRRL+  + E   L   P+GG+MS+I++ + PF HR G +Y+I Y+
Sbjct: 387 FVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYL 446

Query: 444 LGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAYVNYRDLDIGSNNKYGKIDYKQAR 503
             W     + +++++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      +  Y++ +
Sbjct: 447 NYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGK 506

Query: 504 VFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIP 538
            +G KYF NNF RLV+VK+ VDP +FF  EQSIP
Sbjct: 507 SWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210280 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 512.3 bits (1318), Expect = 4.8e-145
Identity = 254/534 (47.57%), Postives = 371/534 (69.48%), Query Frame = 0

Query: 10  SLVFVVVCSSSSLW-VAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSSFSSLLNFP 69
           S +F ++  S ++  + ++   NHE+F++CL +   D     K++HT  +SSF S+L+  
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 70  SRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLSYVSYHQ- 129
            +N RFS   TPKP+ IITP   S +Q  + C++  G+ +R RS GH +EGLSY++Y++ 
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 130 FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFS 189
           F V+DL NLRS+++DV+  T WVQ+GAT GELYY+IGK +++L FPAGI PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 190 GGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 249
           GGGYG+LLRKYG+AADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 250 KLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSAQEGGKIN 309
           KLV VP+T+T+   +++ +++A+++I +WQY A+K+ +DLF+       + + +   K  
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNA 304

Query: 310 PIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEAL 369
             ALF  L++G VN L+A++ + FPELGL KE C+E SWIES    A+    + + L  L
Sbjct: 305 VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVL 364

Query: 370 LNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMSQISESK 429
            NR   T  SFK K D+V+EP+ +AAI+ IWRRLE+ +     + + P+GG+MS+++E +
Sbjct: 365 TNRER-TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYE 424

Query: 430 IPFSHRAGILYQIGYILGW---EEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAYVNYR 489
            PF HR G LY+I Y+  W   E+K      ++L W+  +Y +M P+VSKSPR AYVN++
Sbjct: 425 TPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFK 484

Query: 490 DLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPL 539
           D+D+G      K  Y++ + +G KYF NNF RLV+VK++VDP +FF  EQSIPL
Sbjct: 485 DMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPL 528

BLAST of Chy12G210280 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 509.6 bits (1311), Expect = 3.1e-144
Identity = 256/542 (47.23%), Postives = 369/542 (68.08%), Query Frame = 0

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWVAASSSINHEEFIQCLLHHSPDTYFITKLVHTPIN-- 60
           M +S  +PL  + V+  S  ++   +S +   ++FI C+  ++   + + K    P    
Sbjct: 1   MGISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNV 60

Query: 61  SSFSSLLNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFE 120
           S FS +L   ++N RF   + PKP  I +P + SH+QA+++CSK   + +R+RSGGHD+E
Sbjct: 61  SMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE 120

Query: 121 GLSYVSY--HQFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGI 180
           GLSYVS     FI++DL  +R V ++++ ++AWVQSGAT+GELYY+I +KS+  GFPAG+
Sbjct: 121 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 180

Query: 181 CPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGG 240
           C ++G+GGH +GG YGS++RKYG+ ADNV+DA +VDANG+  DR +MGED FWAIRGG G
Sbjct: 181 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 240

Query: 241 GSFGIVVAWKVKLVPVPATVTICSAKRSLEED-AIKLIDQWQYVANKLEEDLFLAILWNG 300
           GSFGI++AWK+KLVPVP TVT+ +  ++L++D   K+I +WQ VA+KL E+LF+ +L+N 
Sbjct: 241 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFN- 300

Query: 301 GKVSAQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAAN 360
               A  GG       +  LFLG    LM ++ K+FPELGLT ++C E SW+ES A  + 
Sbjct: 301 ---VAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG 360

Query: 361 RFQIEDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIP 420
            F         L  ++P    SFK KSD+VK P+ ++ ++GI+++L  +DI    +   P
Sbjct: 361 -FPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIP--LMIWNP 420

Query: 421 YGGRMSQISESKIPFSHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKS 480
           YGG M++I ES+IPF HR G+L+++ Y+  W +   +   RH+NWIR++YSYM P+VS +
Sbjct: 421 YGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSN 480

Query: 481 PRAAYVNYRDLDIGSNNKYGKIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQS 538
           PR AYVNYRDLD+G N K  K   KQA+V+G+ YF NNFNRL+ +K+KVDP NFF HEQS
Sbjct: 481 PREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQS 534

BLAST of Chy12G210280 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 2.6e-143
Identity = 250/521 (47.98%), Postives = 365/521 (70.06%), Query Frame = 0

Query: 24  VAASSSINHEEFIQCLLHHSPDTYFITKLVHTPINSS--FSSLLNFPSRNSRFSTPNTPK 83
           V  SSS   ++F++CL+ +S  ++ IT    +P  ++  F   L   ++N R+ TP+ PK
Sbjct: 22  VPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPK 81

Query: 84  PLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLSYVSYHQ--FIVVDLINLRSV 143
           P+ I  P   +H+QAAVVC+K   L +R+RSGGHD+EGLS+V+  +  F++VDL  LR V
Sbjct: 82  PVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQV 141

Query: 144 TVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYG 203
            VD++ ++AW  +GAT+GE+YY+I +KS+T GFPAG+C ++G+GGH  GG YGS++RK+G
Sbjct: 142 DVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFG 201

Query: 204 VAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTIC 263
           + ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++AWK+KLVPVPATVT+ 
Sbjct: 202 LGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVF 261

Query: 264 SAKRSLEEDAIKLIDQWQYVANKLEEDLFLAILWNGGKVSAQEGGKINPIALFFTLFLGN 323
           +  ++LE+D  K++ +W+ +A+KL++DLF+ ++ +    + + G +   ++ +   FLG+
Sbjct: 262 TVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMS-YQAQFLGD 321

Query: 324 VNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPITHGSFK 383
            N L+ ++ K+FPELGLTK++C E SWI+S    A  F     P EALL    +    FK
Sbjct: 322 SNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAG-FPNSAAP-EALLAGKSLFKNHFK 381

Query: 384 LKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMSQISESKIPFSHRAGILYQ 443
            KSD+VKEP+    +EG+W R   +D         PYGG MS+ISES+IPF HR G L++
Sbjct: 382 AKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESEIPFPHRNGTLFK 441

Query: 444 IGYILGWEEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAAYVNYRDLDIGSNNKYGKIDY 503
           I ++  W++ G  +E+RH+ WIRE+YSYM  +VSK+PR AYVNYRDLD+G+N   G+ D 
Sbjct: 442 IQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE--GETD- 501

Query: 504 KQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPLSL 541
             AR +G+KY+  NF RLVK+K + DP NFF HEQS+P  +
Sbjct: 502 --AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of Chy12G210280 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 501.5 bits (1290), Expect = 8.4e-142
Identity = 252/540 (46.67%), Postives = 373/540 (69.07%), Query Frame = 0

Query: 6   LIPLSLVFVVVCSSSSLWVAASSS-INHEEFIQCLLHHSPDTYFITKLVHTPINSSFSSL 65
           L+ L + F+++  S S + + S+   NHE F++CL H   +    ++++HT  + S+ S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINED--DSRIIHTSKDPSYFSI 66

Query: 66  LNFPSRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSQGLQIRIRSGGHDFEGLSYVS 125
           LN   +N RF    TPKP+ IITP   + +Q+ + C++  G+ IR RSGGHD+EGLSY++
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 YHQ-FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVG 185
             + F+V+DL NLRS+T+DV+  T WVQSGAT+GELYY+IGK S++L FPAG+ PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 245
           G F GGGYG+L+RKYG++ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFL-AILWNGGKVSAQE 305
           +WK++L+ VP+ VT+    ++ E++A+ +I++WQY+A+K+  DLF+ A+L    +V A  
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASF 306

Query: 306 GGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQ 365
            G          L+LG V++L+A++   FPELGL    C+E SWIES       + I+ +
Sbjct: 307 PG----------LYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL-----WFIKGE 366

Query: 366 PLEALLNRTPITHGSFKLKSDYVKEPMTKAAIEGIWRRLESQDIEGVTLAVIPYGGRMSQ 425
            +E L  R   T  SFK K D+++EP+ K AI+ +WRR E+ +     + + P+GG+MS+
Sbjct: 367 SMEILAKRKR-TSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSE 426

Query: 426 ISESKIPFSHRAGILYQIGYILGW---EEKGVEAEKRHLNWIREIYSYMAPFVSKSPRAA 485
           I++++IPF HR G LY+I Y+  W   E+K     +++L W+  +Y +M P+VSKSPR A
Sbjct: 427 IADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRA 486

Query: 486 YVNYRDLDIGSNNKYG-KIDYKQARVFGSKYFGNNFNRLVKVKSKVDPYNFFWHEQSIPL 539
           YVN+RD+D+G       K  Y++A+V+G KYF NNF+RLV+VK+ VDP +FF  EQSIP+
Sbjct: 487 YVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPI 528