Chy12G210270 (gene) Cucumber (hystrix) v1

BLAST of Chy12G210270 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.0e-107
Identity = 212/514 (41.25%), Postives = 286/514 (55.64%), Query Frame = 0

Query: 35  EDFLQCLLHHSPDANSISKLVYTPFNTSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 94
           E+FL+C   H P+  +  KLVYT  +  Y SILN +I+NLRF +  TPKPL+I+TPSN S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 95  HIQASVICSKSHHLQIRIRSGGHDYEGLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQS 154
           HIQA+++CSK   LQIR RSGGHD EG+SY++ +PF++VDL N+ SI +DV + TAW+++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 155 GATLGELYYRIAEKSRTLAFPAGTSPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 214
           GATLGE+YY I EK+  L+FP G  PTVGVGGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 215 ANGEFLDRESMGRICFGPL--------------------------------EVEAEGALE 274
            +G+ LDR+SMG   F  +                                 +E  G ++
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 275 LW---------------------------RHGR---------TTLANGNI---------- 334
           L+                            HG+         +++ +G +          
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 335 --------------------------------------------------KIKSDYVKEP 394
                                                              IK DYVK+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 395 IPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESEIPFSHRTGNLYKIGYLIRWEE 421
           IP+  +VKI E+L  ED+        PYGG M +ISES IPF HR G +Y++ Y   WE+
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

BLAST of Chy12G210270 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.3e-107
Identity = 215/514 (41.83%), Postives = 281/514 (54.67%), Query Frame = 0

Query: 35  EDFLQCLLHHSPDANSISKLVYTPFNTSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 94
           E+FL+C   + P+  +  KLVYT  N  Y S+LN +I NLRF++  TPKPL+I+TPS++S
Sbjct: 32  ENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVS 91

Query: 95  HIQASVICSKSHHLQIRIRSGGHDYEGLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQS 154
           HIQ +++CSK   LQIR RSGGHD EG+SY++ +PF+IVDL N+RSI +DV + TAW+++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEA 151

Query: 155 GATLGELYYRIAEKSRTLAFPAGTSPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 214
           GATLGE+YY + EK+  L+  AG  PTV  GGHF GGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVN 211

Query: 215 ANGEFLDRESMGRICFGPLE---VEAEGALELWR-------------------------- 274
            +G+ LDR+SMG   F  L     E+ G +  W+                          
Sbjct: 212 VHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKIMEIHELVKL 271

Query: 275 -----------------------------HGRTTLA------------------------ 334
                                         G+   A                        
Sbjct: 272 VNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSF 331

Query: 335 ---------------------------------------------NGNIKIKSDYVKEPI 394
                                                        NG  KIK DYVK+PI
Sbjct: 332 PELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPI 391

Query: 395 PKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESEIPFSHRTGNLYKIGYLIRWEEQ 422
           P+   V+I E+L  EDI        PYGG M +ISES IPF HR G LY++ Y+  WE+Q
Sbjct: 392 PESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQ 451

BLAST of Chy12G210270 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 4.3e-106
Identity = 213/512 (41.60%), Postives = 280/512 (54.69%), Query Frame = 0

Query: 35  EDFLQCLLHHSPDANSISKLVYTPFNTSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 94
           E+FL+C   + P   + +KLVYT  +  Y SILN +I+NLRF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 95  HIQASVICSKSHHLQIRIRSGGHDYEGLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQS 154
           HIQ +++CSK   LQIR RSGGHD EG+SY++ +PF+IVDL N+ S+ +DV + TAW+++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 155 GATLGELYYRIAEKSRTLAFPAGTSPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 214
           GATLGE+YY I E +  L+FPAG  PTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 215 ANGEFLDRESMGRICFGPLE---VEAEGALELWR-------------------------- 274
            +G+ LDR+SMG   F  +     E  G +  W+                          
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 275 ----------------------------------------------HG------------ 334
                                                         HG            
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 335 -----------------------------RTTLANGNI------------KIKSDYVKEP 394
                                         TT     I             IK DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 395 IPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESEIPFSHRTGNLYKIGYLIRWEE 419
           IP+  +V I E+L  ED+ V    F PYGG M +ISES IPF HR G +Y+I Y+  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

BLAST of Chy12G210270 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 9.6e-106
Identity = 211/512 (41.21%), Postives = 278/512 (54.30%), Query Frame = 0

Query: 35  EDFLQCLLHHSPDANSISKLVYTPFNTSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 94
           E+FL+CL  + P   + +KLVYT  +  Y SILN +++NLRF++  TPKPL+I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 95  HIQASVICSKSHHLQIRIRSGGHDYEGLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQS 154
           HIQ +++CSK   LQIR RSGGHD EG+SY++ +PF+IVDL N+ S+ +DV + TAW++S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 155 GATLGELYYRIAEKSRTLAFPAGTSPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 214
           GATLGE+YY I E +  L+FPAG  PTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 215 ANGEFLDRESMGRICFGPLE---VEAEGALELWR-------------------------- 274
            +G+ LDR+SMG   F  +     E  G +  W+                          
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 275 ----------------------------------------------HG------------ 334
                                                         HG            
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 335 -----------------------------------------RTTLANGNIKIKSDYVKEP 394
                                                    R+        IK DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 395 IPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESEIPFSHRTGNLYKIGYLIRWEE 419
           IP+  +V I E+L  ED+ V    F PYGG M +ISES IPF HR G  Y+I Y+  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

BLAST of Chy12G210270 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.4e-104
Identity = 208/514 (40.47%), Postives = 278/514 (54.09%), Query Frame = 0

Query: 35  EDFLQCLLHHSPDANSISKLVYTPFNTSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 94
           E+FL+C   + P+  +  K +YT  +  Y S+LN +I+NLRF++  TPKPL+I+TPSN+S
Sbjct: 32  ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 91

Query: 95  HIQASVICSKSHHLQIRIRSGGHDYEGLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQS 154
           HIQAS++CSK   LQIR RSGGHD EGLSY++ +PF IVDL N+ ++ VD+ + TAW+++
Sbjct: 92  HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 151

Query: 155 GATLGELYYRIAEKSRTLAFPAGTSPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 214
           GATLGE+YY I E +   +FP G  PTVGVGGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 215 ANGEFLDRESMGRICFGPLE---VEAEGALELWR---------------------HGRTT 274
            +G+ LDR+SMG   F  +     E  G +  W+                     HG   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 271

Query: 275 LAN--------------------------------------------------------- 334
           L N                                                         
Sbjct: 272 LFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKS 331

Query: 335 -------------------------------GNIK----------------IKSDYVKEP 394
                                           N K                IK DYVK+ 
Sbjct: 332 FPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKL 391

Query: 395 IPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESEIPFSHRTGNLYKIGYLIRWEE 421
           IP+  +VKI E+L  E++ V      PYGG M +ISES IPF HR G +Y++ Y   WE+
Sbjct: 392 IPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEK 451

BLAST of Chy12G210270 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 9.2e-168
Identity = 319/545 (58.53%), Postives = 361/545 (66.24%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MKFS L+PLSLA   IV+ SS S+    SS + HE+FL CLLHHSPD+ SI+KLVYTP N
Sbjct: 1   MKFSLLLPLSLA--FIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPIN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           TSYS +LNFSIRNLRFSTPNTPKPLLIITPSNISH+QA+VICSKSH +QIRIRSGGHD+E
Sbjct: 61  TSYSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA+ PF++VDLIN+ S+ ++VE +TAW+QSGATLGELYYRIAEKSRTLAFPAG  P
Sbjct: 121 GLSYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICF---------- 240
           TVGVGGHFSGGG+G LLRKYGLAADNVIDAY+VDANGEF DR+SMG   F          
Sbjct: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGS 240

Query: 241 ------------------------GPLEVEAEGALELWRH-------------------- 300
                                     LE EA   ++ W++                    
Sbjct: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKI 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 VAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360

Query: 361 ---------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGR 420
                     RT LANGNIK+KSDYVKEPI K  I ++W+RLKS+DIE  NL F PYGGR
Sbjct: 361 EDQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGR 420

Query: 421 MSQISESEIPFSHRTGNLYKIGYLIRWEEQGVDAEKRHLNWIREIYSYMTPFVSKYPRAA 423
           MSQISESEIPFSHR GNLYKIGYL  W E G++AEK HLNWIR+IYSYMTPFV+K PRAA
Sbjct: 421 MSQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAA 480

BLAST of Chy12G210270 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 9.2e-168
Identity = 319/545 (58.53%), Postives = 361/545 (66.24%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MKFS L+PLSLA   IV+ SS S+    SS + HE+FL CLLHHSPD+ SI+KLVYTP N
Sbjct: 1   MKFSLLLPLSLA--FIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPIN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           TSYS +LNFSIRNLRFSTPNTPKPLLIITPSNISH+QA+VICSKSH +QIRIRSGGHD+E
Sbjct: 61  TSYSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA+ PF++VDLIN+ S+ ++VE +TAW+QSGATLGELYYRIAEKSRTLAFPAG  P
Sbjct: 121 GLSYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICF---------- 240
           TVGVGGHFSGGG+G LLRKYGLAADNVIDAY+VDANGEF DR+SMG   F          
Sbjct: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGS 240

Query: 241 ------------------------GPLEVEAEGALELWRH-------------------- 300
                                     LE EA   ++ W++                    
Sbjct: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKI 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 VAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360

Query: 361 ---------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGR 420
                     RT LANGNIK+KSDYVKEPI K  I ++W+RLKS+DIE  NL F PYGGR
Sbjct: 361 EDQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGR 420

Query: 421 MSQISESEIPFSHRTGNLYKIGYLIRWEEQGVDAEKRHLNWIREIYSYMTPFVSKYPRAA 423
           MSQISESEIPFSHR GNLYKIGYL  W E G++AEK HLNWIR+IYSYMTPFV+K PRAA
Sbjct: 421 MSQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAA 480

BLAST of Chy12G210270 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.1e-157
Identity = 305/541 (56.38%), Postives = 353/541 (65.25%), Query Frame = 0

Query: 1   MKFSS-LIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDAN-SISKLVYTP 60
           MK+SS LIPLS +++ I + SS    A  +S  KHE FL CLL HSPD+N SISK+VYTP
Sbjct: 1   MKYSSPLIPLSFSLLFIFLSSSSLWAA--ASVDKHESFLNCLLDHSPDSNYSISKVVYTP 60

Query: 61  FNTSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHD 120
            N+SYSS+L+FSIRNLRFST +TPKPL IITP ++SH+QA+V+CS++H LQIR RSGGHD
Sbjct: 61  LNSSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHD 120

Query: 121 YEGLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGT 180
           +EGLS V+HLPFII+DLIN+RSI +D   NTAW+QSGATLGELYY IAEKSRTLAFPAG 
Sbjct: 121 FEGLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGA 180

Query: 181 SPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFG------- 240
            PTVG+GGHFSGGGYG++LRK+GLAADNVIDAYLVDANG+F DRE MG   F        
Sbjct: 181 CPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGG 240

Query: 241 ----------------PLEVEA--------EGALEL---WRH------------------ 300
                           P  V +        EGA++L   W++                  
Sbjct: 241 GSFGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGG 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 NFTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGF 360

Query: 361 ----------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGG 418
                      RT L NG+IKIKSDYVKEPI +  I  IWERLKS D+    L F PYGG
Sbjct: 361 KIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGG 420

BLAST of Chy12G210270 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 9.5e-157
Identity = 306/540 (56.67%), Postives = 349/540 (64.63%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           M  S L+PLSL  + IV+ SS SL A  SS IKHE+FLQCLLHHSPD +SI+KLV+TP N
Sbjct: 14  MMLSPLVPLSL--VFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPIN 73

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           +S+S++ NF  RN RFSTPNTPKPLLIITPSNISHIQA+VICSKSH LQIRIRSGGHD+E
Sbjct: 74  SSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFE 133

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA+  FI+VDLIN+RS+ VDVE +TAW+QSGATLGELYY+I +KSRTL FPAG  P
Sbjct: 134 GLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICP 193

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGR------------- 240
           TVGVGGHFSGGGYG LLRKYG+AADNVIDAYLVDANGEF DRESMG              
Sbjct: 194 TVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGS 253

Query: 241 -------------------ICFGPLEVEAE------------GALE-------LWRHGRT 300
                              IC     VE E              LE       LW  G+ 
Sbjct: 254 FGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKV 313

Query: 301 TLANG------------------------------------------------------- 360
           +   G                                                       
Sbjct: 314 SAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQ 373

Query: 361 -------------------NIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYG 416
                              + K+KSDYVKEP+ K  I  IW+RL+S+DIE V L   PYG
Sbjct: 374 IEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYG 433

BLAST of Chy12G210270 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 6.8e-155
Identity = 299/541 (55.27%), Postives = 344/541 (63.59%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MK+S L  LSLA   I++ SS S  A ++   KHE FLQCL HHS D++SISK++YTP N
Sbjct: 1   MKYSPLTILSLA--FILLSSSSSWAAASAE--KHEAFLQCLSHHSADSSSISKVIYTPIN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
            SYSS+LNFS+RNLRFS+  TPKPLLIITPS +SH+QA+VICSK+H LQIRIRSGGHD E
Sbjct: 61  PSYSSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVAHLPFII+DLIN++SI V++E +TAW+QSGATLGELYY IA+KSRTLAFPAG  P
Sbjct: 121 GLSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGP-------- 240
           TVGVGGHFSGGGYG++LRKYGLAADNVIDAYL+DANG+F DRESMG   F          
Sbjct: 181 TVGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGS 240

Query: 241 --------------------------LEVEAEGALELWRH-------------------- 300
                                     L+ +    +  W+                     
Sbjct: 241 FGIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNA 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 STEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRN 360

Query: 361 ----------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGG 418
                      RT L   N K+KSDYVKEPIPK  I  IWERLK++DIEV  L F PYGG
Sbjct: 361 QTIESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGG 420

BLAST of Chy12G210270 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 615 bits (1587), Expect = 1.66e-216
Identity = 336/544 (61.76%), Postives = 370/544 (68.01%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MKFS LIPLSLA II+   SS  L A +SS+IK+E+FLQCLLHHSP+  SI+ LVY P N
Sbjct: 1   MKFSPLIPLSLAFIILC-SSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           +SYSS+LNFSIRNLRFSTP  PKPLLIITPSNISHIQA+V+CSKSH LQIRIRSGGHD+E
Sbjct: 61  SSYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA+ PFI+VDLIN+ S+AVDVE +TAW+QSGATLGELYYRIAEKSRTLAFPAGT P
Sbjct: 121 GLSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGPLE------ 240
           TVGVGGHFSGGGYG LLRKYGLAADNVIDAYLVDANGEF DRESMG   F  +       
Sbjct: 181 TVGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGS 240

Query: 241 -------------VEA------------EGALEL---WRH-------------------- 300
                        V A            EGA++L   W++                    
Sbjct: 241 FGIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKI 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 SAQGGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 360

Query: 361 ---------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGR 420
                     RT LANG+ KIKSDYVKEPI K  I  IW+RLKS+DIE VNLG  PYGGR
Sbjct: 361 DDQPLEAFLNRTPLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGGR 420

BLAST of Chy12G210270 vs. NCBI nr
Match: XP_011659204.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653317.1 hypothetical protein Csa_023357 [Cucumis sativus])

HSP 1 Score: 595 bits (1533), Expect = 2.56e-208
Identity = 321/545 (58.90%), Postives = 360/545 (66.06%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MKFS L+PLSLA   I + SS S+    SS + HE+FLQCLLHHSP + SI+KL YTP N
Sbjct: 1   MKFSLLLPLSLA--FIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPIN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           TSYSS+LNFSIRNLRFS PNTPKPL IITP+NISH+QA+VICSKSH LQIRIRSGGHD+E
Sbjct: 61  TSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA+ PFIIVDLI++ S+ ++V+ +TAW+QSGATLGELYYRIAEKSRTLAFPAG SP
Sbjct: 121 GLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGP-------- 240
           TVGVGGHFSGGG+G LLRKYGLAADNVIDAYLVDANG F DR+SMG   F          
Sbjct: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGS 240

Query: 241 --------------------------LEVEAEGALELWRH-------------------- 300
                                     LE EA   ++ W++                    
Sbjct: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKI 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 SAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360

Query: 361 ---------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGR 420
                     RT LA GNIK+KSDYVKEPIPK  I +IW+RL+S+DIE  NL F PYGGR
Sbjct: 361 EDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420

Query: 421 MSQISESEIPFSHRTGNLYKIGYLIRWEEQGVDAEKRHLNWIREIYSYMTPFVSKYPRAA 422
           MSQIS+SEIPFSHR GNLYKIGYL  W E GV+AEK HLNWIR+IY YMTPFVSK PRAA
Sbjct: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480

BLAST of Chy12G210270 vs. NCBI nr
Match: XP_008445709.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo] >KAA0047097.1 cannabidiolic acid synthase-like [Cucumis melo var. makuwa] >TYK05061.1 cannabidiolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 593 bits (1529), Expect = 1.04e-207
Identity = 319/545 (58.53%), Postives = 361/545 (66.24%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MKFS L+PLSLA   IV+ SS S+    SS + HE+FL CLLHHSPD+ SI+KLVYTP N
Sbjct: 1   MKFSLLLPLSLA--FIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPIN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           TSYS +LNFSIRNLRFSTPNTPKPLLIITPSNISH+QA+VICSKSH +QIRIRSGGHD+E
Sbjct: 61  TSYSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA+ PF++VDLIN+ S+ ++VE +TAW+QSGATLGELYYRIAEKSRTLAFPAG  P
Sbjct: 121 GLSYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGP-------- 240
           TVGVGGHFSGGG+G LLRKYGLAADNVIDAY+VDANGEF DR+SMG   F          
Sbjct: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGS 240

Query: 241 --------------------------LEVEAEGALELWRH-------------------- 300
                                     LE EA   ++ W++                    
Sbjct: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKI 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 VAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360

Query: 361 ---------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGR 420
                     RT LANGNIK+KSDYVKEPI K  I ++W+RLKS+DIE  NL F PYGGR
Sbjct: 361 EDQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGR 420

Query: 421 MSQISESEIPFSHRTGNLYKIGYLIRWEEQGVDAEKRHLNWIREIYSYMTPFVSKYPRAA 422
           MSQISESEIPFSHR GNLYKIGYL  W E G++AEK HLNWIR+IYSYMTPFV+K PRAA
Sbjct: 421 MSQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAA 480

BLAST of Chy12G210270 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 592 bits (1525), Expect = 4.50e-207
Identity = 325/547 (59.41%), Postives = 362/547 (66.18%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MKFS LI LSLA  IIV  SS  L A +SS+IKHE+FLQCLLHHSP+  SI+KLVY P N
Sbjct: 1   MKFSPLILLSLA-FIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPIN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           +SYSS+LNF  RN RFSTPN P PLLIITP+NISHIQA++ICSKSH LQIRIRSGGHD+E
Sbjct: 61  SSYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA++PFI+VDLIN+RS+ VD E ++AW+QSGATLGELYYRIAEKS+TLAFPAG  P
Sbjct: 121 GLSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGR------------- 240
           TVGVGGHFSGGGYG+LLRKYGLAADNVIDAYLVDANGEF DRESMG              
Sbjct: 181 TVGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGN 240

Query: 241 -------------------ICFGPLEVEAEGALEL---WRH------------------- 300
                              IC     +E EGA+EL   W++                   
Sbjct: 241 FGVVVAWKVKLVPVPETVTICAAKRTLE-EGAIELIDQWQYVGNKLEEDLFLSIFLNGGK 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 NSAQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRF 360

Query: 361 -----------GRTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYG 420
                       RT LANG++KIKSDYVKE I K  I  IWERLKS+DIE V L   PYG
Sbjct: 361 QIEDQPLEALLNRTPLANGSLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYG 420

Query: 421 GRMSQISESEIPFSHRTGNLYKIGYLIRWEEQGVDAEKRHLNWIREIYSYMTPFVSKYPR 422
           GRM QIS+SEIPFSHR G  Y+IGY++ W+EQG+DAEKRH+NWIREIYSYMTPFVSK PR
Sbjct: 421 GRMGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPR 480

BLAST of Chy12G210270 vs. NCBI nr
Match: XP_038895570.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 564 bits (1454), Expect = 2.35e-196
Identity = 311/545 (57.06%), Postives = 359/545 (65.87%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           MK S LI L L++++I++ SS    A NSS  KHE+FL+CL HHSP+   ISK++YTPFN
Sbjct: 1   MKSSPLI-LLLSLVVILLSSSAPWVASNSSE-KHENFLECLFHHSPN---ISKVIYTPFN 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           +SYSSILNF++RNLRFSTPNTPKPLLIITPS+ISHIQ +VICSKS+ LQIR RSGGHD+E
Sbjct: 61  SSYSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSP 180
           GLSYVA+LPFIIVDLIN+ SI +++ETNTAW++SGATLGELYYRIAEKSRTLAFPAGT P
Sbjct: 121 GLSYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCP 180

Query: 181 TVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGR------------- 240
            VGVGGH SGGGYGML+RKYGLAADNVIDAYLVDANGEF DRESMG              
Sbjct: 181 IVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGS 240

Query: 241 -------------------ICFGPLEVEAEGALEL---WRH------------------- 300
                              +CF     E EGA  L   W++                   
Sbjct: 241 FGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATNLIHRWQYVAHDLDENLFIGILLSGGN 300

Query: 301 -----GRT---------------------------------------------------- 360
                G+T                                                    
Sbjct: 301 FTRQEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQ 360

Query: 361 ------------TLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGR 420
                       +L NGN KIKSDY+K+PIP   +  IW+RLK EDIE+  L F PYGGR
Sbjct: 361 PGKSLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGR 420

Query: 421 MSQISESEIPFSHRTGNLYKIGYLIRWEEQGVDAEKRHLNWIREIYSYMTPFVSKYPRAA 422
           MSQISESE PFSHR G LYKIGY+  WE+Q  D EKRHL+WIRE+YSYMTPFVSK PRAA
Sbjct: 421 MSQISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAA 480

BLAST of Chy12G210270 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 372.9 bits (956), Expect = 3.5e-103
Identity = 211/534 (39.51%), Postives = 287/534 (53.75%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAP---NSSFIKHEDFLQCLLHHSPDANSISKLVYT 60
           MKF S      +V+I    S+  L  P   +++      FLQCL     D+N +SK+++T
Sbjct: 1   MKFQSFFS---SVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHT 60

Query: 61  PFNTSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGH 120
           P +TS+SS+L  SI+N RFS P+ PKP+LI+TP   S +Q++V C++   + IR RSGGH
Sbjct: 61  PNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGH 120

Query: 121 DYEGLSYVAHLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAG 180
           DYEGLSYV H PF+I+DL N+RSI VDV+  + W+Q+GAT+GELYY I +K+RTLAFPAG
Sbjct: 121 DYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAG 180

Query: 181 TSPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGR---------- 240
             PTVGVGGHFSGGGYG LLRK+GLAAD+VIDA +VDA G  L+R  MG           
Sbjct: 181 VCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGG 240

Query: 241 ---IC------------------------------------------------------- 300
               C                                                       
Sbjct: 241 GSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQR 300

Query: 301 ---------------------------FGPLEVEAEGALEL-WRHG-------------- 360
                                      F  L +E +   E+ W                 
Sbjct: 301 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPINV 360

Query: 361 --RTTLANGNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESE 420
             + T A+   K KSD+V+EP+PK  I K+W RL+  + E   L FTP+GG+MS+I++ E
Sbjct: 361 LTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYE 420

BLAST of Chy12G210270 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 372.1 bits (954), Expect = 6.0e-103
Identity = 207/529 (39.13%), Postives = 292/529 (55.20%), Query Frame = 0

Query: 9   LSLAVIIIVIRSS----PSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFNTSYS 68
           LSL +  +++  S    PS+ A  ++   HE+FL+CL H   + +  S++++T  + SY 
Sbjct: 8   LSLFIYFLLLNLSLSHFPSISAQRTN---HENFLKCLSHRINEDD--SRIIHTSKDPSYF 67

Query: 69  SILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYEGLSY 128
           SILN SI+N RF    TPKP+ IITP   + +Q+++ C++ H + IR RSGGHDYEGLSY
Sbjct: 68  SILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSY 127

Query: 129 VA-HLPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSPTVG 188
           +A   PF+++DL N+RSI +DV+  T W+QSGAT+GELYY I + S++LAFPAG  PTVG
Sbjct: 128 MAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVG 187

Query: 189 VGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGPL---------- 248
           +GG F GGGYG L+RKYGL+ADNVIDA++VDANG FLDR+ MG   F  +          
Sbjct: 188 IGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSV 247

Query: 249 ------------------------EVEAEGALELWRHGRTTLAN---------------- 308
                                   E EA   +  W++    + N                
Sbjct: 248 VLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYA 307

Query: 309 ----------------------------GN------------------------------ 368
                                       GN                              
Sbjct: 308 SFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKGESMEILAKRKRTSR 367

Query: 369 -IKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESEIPFSHRTGN 420
             K K D+++EPIPK  I  +W R ++ +  +  +  TP+GG+MS+I+++EIPF HR GN
Sbjct: 368 SFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGN 427

BLAST of Chy12G210270 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 359.4 bits (921), Expect = 4.0e-99
Identity = 202/533 (37.90%), Postives = 287/533 (53.85%), Query Frame = 0

Query: 1   MKFSSLIPLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN 60
           M+FSS +     +++  +  SP + A  S+   HEDFL+CL +   D     K+++T  +
Sbjct: 1   MEFSSFL---FTILLFSLNISPLVSAHGSN---HEDFLKCLSYRMNDNTVEPKVIHTSKD 60

Query: 61  TSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYE 120
           +S+ SIL+ SI+N RFS   TPKP+ IITP   S +Q  + C++ H + +R RS GH YE
Sbjct: 61  SSFFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYE 120

Query: 121 GLSYVAH-LPFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTS 180
           GLSY+A+  PF ++DL N+RSI++DV+  T W+Q+GAT GELYY I + +++LAFPAG  
Sbjct: 121 GLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIH 180

Query: 181 PTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGPL------ 240
           PTVGVGG FSGGGYG LLRKYGLAADN+IDA +VDA+G  LDR++MG   F  +      
Sbjct: 181 PTVGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGS 240

Query: 241 ----------------------------EVEAEGALELWRHG----------RTTLANGN 300
                                       + EA   ++ W++           RTTL   N
Sbjct: 241 SFGVILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGV 360

Query: 361 ----------IKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESE 416
                      K K D+V+EPIP+  I +IW RL++ +  +  +  TP+GG+MS+++E E
Sbjct: 361 LTNRERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYE 420

BLAST of Chy12G210270 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.9e-96
Identity = 202/537 (37.62%), Postives = 285/537 (53.07%), Query Frame = 0

Query: 8   PLSLAVIIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFN--TSYSS 67
           PL L  I+++  S  ++   +S     + F+ C+  ++     + K  + P    + +S 
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQ 65

Query: 68  ILNFSIRNLRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYEGLSYV 127
           +L  + +NLRF   + PKP  I +P + SH+QAS+ICSK   + +R+RSGGHDYEGLSYV
Sbjct: 66  VLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYV 125

Query: 128 AHL--PFIIVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSPTVG 187
           + +  PFI++DL  +R + ++++ N+AW+QSGAT+GELYYRIAEKS+   FPAG   ++G
Sbjct: 126 SQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLG 185

Query: 188 VGGHFSGGGYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGPLEVEAEGA--- 247
           +GGH +GG YG ++RKYGL ADNV+DA +VDANG+ LDR +MG   F  +   A G+   
Sbjct: 186 IGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGI 245

Query: 248 --------------------------------LELWRH---------------------- 307
                                           +  W+                       
Sbjct: 246 ILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGG 305

Query: 308 --------------GRTTLAN--------------------------------------- 367
                         G+ TL N                                       
Sbjct: 306 NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNV 365

Query: 368 ----------GNIKIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISE 418
                      + K KSD+VK PIP+  +  I+++L  EDI +  + + PYGG M++I E
Sbjct: 366 LLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPE 425

BLAST of Chy12G210270 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-95
Identity = 201/527 (38.14%), Postives = 278/527 (52.75%), Query Frame = 0

Query: 14  IIIVIRSSPSLGAPNSSFIKHEDFLQCLLHHSPDANSISKLVYTPFNTSYSSILNFSIRN 73
           +I+V+     +   +SS    E F QCL  +S   + IS  ++   N SYSS+L  +IRN
Sbjct: 5   LILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRN 64

Query: 74  LRFSTPNTPKPLLIITPSNISHIQASVICSKSHHLQIRIRSGGHDYEGLSYVAH--LPFI 133
           LRF+T +TPKP LII  ++ SH+QA++ C K H+LQ++IRSGGHDY+GLSYV +   PF 
Sbjct: 65  LRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFF 124

Query: 134 IVDLINIRSIAVDVETNTAWLQSGATLGELYYRIAEKSRTLAFPAGTSPTVGVGGHFSGG 193
           ++D+ N+RS+ VDV + TAW+Q+GA LGE+YY I EKS+TLA+PAG  PTVGVGGH SGG
Sbjct: 125 VLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGG 184

Query: 194 GYGMLLRKYGLAADNVIDAYLVDANGEFLDRESMGRICFGPLE----------------- 253
           GYG ++RKYGL  DN IDA +VD NG+ LDR+ MG   +  +                  
Sbjct: 185 GYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINL 244

Query: 254 VEAEGALELWRHGRT-----------------------------TLANGNI--------- 313
           VE    + ++R  RT                              + NG +         
Sbjct: 245 VEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 304

Query: 314 ------------------------------------------------------------ 373
                                                                       
Sbjct: 305 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQP 364

Query: 374 ----KIKSDYVKEPIPKPKIVKIWERLKSEDIEVVNLGFTPYGGRMSQISESEIPFSHRT 419
               K KSDYV+EPI +  +  IW+  K  ++E+  + F PYGG M +IS +  PF +R 
Sbjct: 365 VNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 424