Chy12G210210 (gene) Cucumber (hystrix) v1

Overview
NameChy12G210210
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionberberine bridge enzyme-like 18
LocationchrH12: 5109357 .. 5112824 (+)
RNA-Seq ExpressionChy12G210210
SyntenyChy12G210210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCTTCTTCCCAATTAATCCCTCTTTTTCTTGTACTCATTATTGTTTTATCTGCATCTCCTTCATGGGTTTCAGCATTTCCCCATAAACATCAAAACTTTGTTCAATGTCTTTCAGAGCATTCTTCAAAGAGTTATCCCATTTCCAAAGTGGTTTATACTCCAATCAACTCTTCTTATTCTTCTGTTTTGAACTTCTCCATTCGAAACCTCCGATTCTCAAAGCCTGAAACCCCAAAGCCACTCCTTATTATCACTCCCTCACATGTTTCCCACATTCAGGCAGCTGTCATATGCTCCAAATCCCATGGCTTACAAATCCGAACTCGAAGCGGCGGCCATGACTACGAGGGTCTTTCTTACGTTGCCTATCACCCATTTATCATCGTTGACCTAATAAATCTCAGGTTCGTTGTCGTTCTCTTTCTTTTCTTTTATTTTTTTTTTCAAGTATAATAAGAGGCAGTATGAGGTAGTACCTATCGATTACCGTTAATCTATCTTTATTTGAGACCATGTCTATTACGTTTTTTCATTATGAGTGGGGTACGAGGAAATACATTTCGATATTGCTATTGAACTACGCTTACTTTTGTTCAAGTCTGTTCCTCTCTGTTTGCTTGTTTTGTTAGTGTAATAAGTGTAGATATGAAGTATTGCATGTCGACTACTGTTGAGTTATGCTTATTTTGGATTAAGTTTGTTCAATAAGTAATGAGATATTACATGTCTAATTAAGTTCTATGTTCACTTTGACTCAGATCCATTTCTATTGACATTGTAAACAACACTGCATGGGTTGAGTCAGGTGCAAGTCTAGGCCAACTTTACTATAGAATTGGTGAAAAAAGTCGAACCTTGGCATTTCCAGCGGGTATTTGTCCAACAGTTGGCGTCGGTGGGCATTTCAGCGGCGGTGGATATGGATTCCTGCTGAGGAAATATGGACTTGCTGCTGATAATGTGATAGATGCATATTTGGTTGATGCAAATGGGATGGTTCATGACAGGGAATCGATGGGAGAGGATTTGTTTTGGGCCATTAGAGGCGGCAGCGGGGGGAGCTTTGGAATTGTGGTGGCCTGGAAGGTCAAGCTGGTACCGGTTCCGGCCATGGTAACGATTTGCTCTACTATAAAAAATTTGGAGGATGATACAGTTAAGGTGATCCATCAGTGGCAATATGTGGCTAACAAATTACATGAAGATATATTCCTTGGCATCGTTTTGACTGGTATTTTTTTCTAAAGCACATGTTTTATTTTTTTATTCTTTTTAACTATTTATTGTCTTTATTTTCTTTGGAAAATATGTTTCTTAGATTAATATTATAATGTGAATTAAACTACACGACATTATTTAAATAAATTGTGTGATGTGTGTTTTTTTGTTCGGTTTAAAAATATATATACACACATACATACATATATATTTTATTAAATAAGAGTTTTGCAGCTATATAGTTTTCTATTTGCTTATAAATAAATTATAGAAAGTTGTTGAATGTGCTAGAGTGATAATTGTTGAGCAGTACTAGAGGGTAGAGAAATAAAAGTGTAAGGGAGAAAAATTTAGCAAAAATAAAGATTAAGTTAGAAAAGAAATAAAACTTCACCTTCGCATTTGTGTCCAAAAATACTATCACTCAAGTTTCTTAACTTTAAAGAAAGTTAAATATTGTTATAGTTAGTTTTTGTTTTTTTAAGGGAGACAAAGTGTCAGCCATGACTTTAGGAAAACCCTCTTGGATAACCTAAAGAGCTAACAACATACTATAATTTATTGATAGGTTGATAAAGTAATTACATAAAAGGTTAGAGGGATCCAAGAGAGGAAAGCTTTTAATTGAGCAAAGCTTGGAAATTTAGAGCTATAGTGTTAGGACTATAGTCTTTAAAAAGATGGTGTCTACTGCATCAAAGACCTATGTAGTTACAAGAGTCTTTGTATAAATTTAATGAACTTTTTGTGTTTGTTGGTGAAGATTCTTTTCTTTCTTTCATTCAAATGTACCAAAGAATTGTCACTTTTTTATAGTTAGTTTTAGATAGAAATGATTAATATTTTTGTTTCAAAACGAAGTTCCTTAAATTTTTCACAAAAGTTTGAAAAGTATAATTAAACTTAAAAGAAAGGTTAAAAACTGGTGTATTGTTAATATATAAATTAAAATGTTAATATCTCATGGTAAAAAATATCTTATGCTTTCAGAAAATCACTTTTTTATTTTTTATTTTTTGGTAGGGATCAGAGAATTTAATGCAGTTTTTAAAAGTTGAATAGTACTTTTCAAACCTATAGTGTATATGCACTTGGGAAAGTTAAGAGAATCTCAAATATTTACACCCCTTTATTTTATTTCATTTTTTTTCCGTTTTGGTTCAGCACTATCTAATTCGGTAAGTGAGAAATATGTCGACACATTCCATTTTTAATGTTATGAAATTAAACATATTTAGAAATTGATTTTTGGATAAGTAATTTCTTACCTTAGGTTTAATGTTGGCGTCTTATGTATTAAGTTTTACTTAGATTTTGAAGAGGGATAATAATTGACCATTTAGTATAAATCACATATTTCTAAATCTGTTTACTAACTCTGAACTTTCCTACCTCAAAATTACAAAATTGTTCATAAAATTATAAAATAAAATATGACTTCCAACATCCAAAGCAACTCACCTCAATAAAAATATGGATGATCTGAAAGCTCATTTCTTGATATATGTAGGTGGAAATACTTCAACTCAAGGAGGCAAAAAGAACCCAATAGCAACATTCTACTCATTATTTCTTGGAAAGGTAGATGAACTTGTGGCAACTTTATCAACAACATTTCCTGAATTAGGTTTAATAAAACAAGATTGTCTCGAAGTGAGATGGGTCGAATCAACCCTCATCATCGCCTCTGGAGTATTTCAAACCGTCGAGTCATTAGAACCTTTACTCAATAGAACACCTACCACCCTCGAAAGCACAAAAATCAAATCAGACTACATCAAGGAGCCCATCCCCAAGGCAGCAATTGAGGGCATATGGCAAAGATTAAAGGCTCGTGATATAGAAGGACCACAATTCGTTTTTGCACCTTATGGGGCGAGAATGAGTCAAATTTCTGAATCAGAAACTCCATTTTCGCATAGAGCTGGATATTTATACCAGATTGGGTACATGGTAGGTTGGAAGGGACAAAATTTAAAGGCAAAAAATAGACATATAAGTTGGATACGAGAGCTTTATGAGTACATGGCTCCTTTTGTTTCAAAATCACCAAGGGCTGCATATGCTAATTATAGAGATCTTGATATTGGAAGTAACAACAAATATGGAAAAACAAGCTATAAGCAAGCAAGCATTTGGGGGTTGAAGTATTTTGGGAACAATTTTAAGAGATTGGTATATGTTAAAACTAAGGTTGATCCTCATGATTTCTTTAGGCATGAACAAAGTATACCTACCCTCTAG

mRNA sequence

ATGAAGTCTTCTTCCCAATTAATCCCTCTTTTTCTTGTACTCATTATTGTTTTATCTGCATCTCCTTCATGGGTTTCAGCATTTCCCCATAAACATCAAAACTTTGTTCAATGTCTTTCAGAGCATTCTTCAAAGAGTTATCCCATTTCCAAAGTGGTTTATACTCCAATCAACTCTTCTTATTCTTCTGTTTTGAACTTCTCCATTCGAAACCTCCGATTCTCAAAGCCTGAAACCCCAAAGCCACTCCTTATTATCACTCCCTCACATGTTTCCCACATTCAGGCAGCTGTCATATGCTCCAAATCCCATGGCTTACAAATCCGAACTCGAAGCGGCGGCCATGACTACGAGGGTCTTTCTTACGTTGCCTATCACCCATTTATCATCGTTGACCTAATAAATCTCAGATCCATTTCTATTGACATTGTAAACAACACTGCATGGGTTGAGTCAGGTGCAAGTCTAGGCCAACTTTACTATAGAATTGGTGAAAAAAGTCGAACCTTGGCATTTCCAGCGGGTATTTGTCCAACAGTTGGCGTCGGTGGGCATTTCAGCGGCGGTGGATATGGATTCCTGCTGAGGAAATATGGACTTGCTGCTGATAATGTGATAGATGCATATTTGGTTGATGCAAATGGGATGGTTCATGACAGGGAATCGATGGGAGAGGATTTGTTTTGGGCCATTAGAGGCGGCAGCGGGGGGAGCTTTGGAATTGTGGTGGCCTGGAAGGTCAAGCTGGTACCGGTTCCGGCCATGGTAACGATTTGCTCTACTATAAAAAATTTGGAGGATGATACAGTTAAGGTGATCCATCAGTGGCAATATGTGGCTAACAAATTACATGAAGATATATTCCTTGGCATCGTTTTGACTGGTGGAAATACTTCAACTCAAGGAGGCAAAAAGAACCCAATAGCAACATTCTACTCATTATTTCTTGGAAAGGTAGATGAACTTGTGGCAACTTTATCAACAACATTTCCTGAATTAGGTTTAATAAAACAAGATTGTCTCGAAGTGAGATGGGTCGAATCAACCCTCATCATCGCCTCTGGAGTATTTCAAACCGTCGAGTCATTAGAACCTTTACTCAATAGAACACCTACCACCCTCGAAAGCACAAAAATCAAATCAGACTACATCAAGGAGCCCATCCCCAAGGCAGCAATTGAGGGCATATGGCAAAGATTAAAGGCTCGTGATATAGAAGGACCACAATTCGTTTTTGCACCTTATGGGGCGAGAATGAGTCAAATTTCTGAATCAGAAACTCCATTTTCGCATAGAGCTGGATATTTATACCAGATTGGGTACATGGTAGGTTGGAAGGGACAAAATTTAAAGGCAAAAAATAGACATATAAGTTGGATACGAGAGCTTTATGAGTACATGGCTCCTTTTGTTTCAAAATCACCAAGGGCTGCATATGCTAATTATAGAGATCTTGATATTGGAAGTAACAACAAATATGGAAAAACAAGCTATAAGCAAGCAAGCATTTGGGGGTTGAAGTATTTTGGGAACAATTTTAAGAGATTGGTATATGTTAAAACTAAGGTTGATCCTCATGATTTCTTTAGGCATGAACAAAGTATACCTACCCTCTAG

Coding sequence (CDS)

ATGAAGTCTTCTTCCCAATTAATCCCTCTTTTTCTTGTACTCATTATTGTTTTATCTGCATCTCCTTCATGGGTTTCAGCATTTCCCCATAAACATCAAAACTTTGTTCAATGTCTTTCAGAGCATTCTTCAAAGAGTTATCCCATTTCCAAAGTGGTTTATACTCCAATCAACTCTTCTTATTCTTCTGTTTTGAACTTCTCCATTCGAAACCTCCGATTCTCAAAGCCTGAAACCCCAAAGCCACTCCTTATTATCACTCCCTCACATGTTTCCCACATTCAGGCAGCTGTCATATGCTCCAAATCCCATGGCTTACAAATCCGAACTCGAAGCGGCGGCCATGACTACGAGGGTCTTTCTTACGTTGCCTATCACCCATTTATCATCGTTGACCTAATAAATCTCAGATCCATTTCTATTGACATTGTAAACAACACTGCATGGGTTGAGTCAGGTGCAAGTCTAGGCCAACTTTACTATAGAATTGGTGAAAAAAGTCGAACCTTGGCATTTCCAGCGGGTATTTGTCCAACAGTTGGCGTCGGTGGGCATTTCAGCGGCGGTGGATATGGATTCCTGCTGAGGAAATATGGACTTGCTGCTGATAATGTGATAGATGCATATTTGGTTGATGCAAATGGGATGGTTCATGACAGGGAATCGATGGGAGAGGATTTGTTTTGGGCCATTAGAGGCGGCAGCGGGGGGAGCTTTGGAATTGTGGTGGCCTGGAAGGTCAAGCTGGTACCGGTTCCGGCCATGGTAACGATTTGCTCTACTATAAAAAATTTGGAGGATGATACAGTTAAGGTGATCCATCAGTGGCAATATGTGGCTAACAAATTACATGAAGATATATTCCTTGGCATCGTTTTGACTGGTGGAAATACTTCAACTCAAGGAGGCAAAAAGAACCCAATAGCAACATTCTACTCATTATTTCTTGGAAAGGTAGATGAACTTGTGGCAACTTTATCAACAACATTTCCTGAATTAGGTTTAATAAAACAAGATTGTCTCGAAGTGAGATGGGTCGAATCAACCCTCATCATCGCCTCTGGAGTATTTCAAACCGTCGAGTCATTAGAACCTTTACTCAATAGAACACCTACCACCCTCGAAAGCACAAAAATCAAATCAGACTACATCAAGGAGCCCATCCCCAAGGCAGCAATTGAGGGCATATGGCAAAGATTAAAGGCTCGTGATATAGAAGGACCACAATTCGTTTTTGCACCTTATGGGGCGAGAATGAGTCAAATTTCTGAATCAGAAACTCCATTTTCGCATAGAGCTGGATATTTATACCAGATTGGGTACATGGTAGGTTGGAAGGGACAAAATTTAAAGGCAAAAAATAGACATATAAGTTGGATACGAGAGCTTTATGAGTACATGGCTCCTTTTGTTTCAAAATCACCAAGGGCTGCATATGCTAATTATAGAGATCTTGATATTGGAAGTAACAACAAATATGGAAAAACAAGCTATAAGCAAGCAAGCATTTGGGGGTTGAAGTATTTTGGGAACAATTTTAAGAGATTGGTATATGTTAAAACTAAGGTTGATCCTCATGATTTCTTTAGGCATGAACAAAGTATACCTACCCTCTAG

Protein sequence

MKSSSQLIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTVESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL*
Homology
BLAST of Chy12G210210 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 2.1e-150
Identity = 262/527 (49.72%), Postives = 367/527 (69.64%), Query Frame = 0

Query: 10  LFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSI 69
           +F     +L + P  VSA      +F+QCLS   + S  +SKV++TP ++S+SSVL  SI
Sbjct: 12  IFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSI 71

Query: 70  RNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYHPFI 129
           +N RFS P+ PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHDYEGLSYV + PF+
Sbjct: 72  QNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFV 131

Query: 130 IVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSGG 189
           I+DL NLRSI++D+ N + WV++GA++G+LYY IG+K+RTLAFPAG+CPTVGVGGHFSGG
Sbjct: 132 ILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGG 191

Query: 190 GYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKL 249
           GYG LLRK+GLAAD+VIDA +VDA G + +R  MGED FWAIRGG G SF +V++WK+ L
Sbjct: 192 GYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGL 251

Query: 250 VPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGKKNPIA 309
           + VP+ VT+ +  K  E   +K+IH+WQ+VA+K+ +D+F+ ++L       Q  K    A
Sbjct: 252 INVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYKNMVRA 311

Query: 310 TFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTVESLEPLLNR 369
           +F  L+LG V  L+  ++  FPELGL + DC E+ W+ES +  A       E +  L  R
Sbjct: 312 SFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE---LGEEPINVLTKR 371

Query: 370 TPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESETPF 429
           T  +L + K KSD+++EP+PK AI  +W+RL+  + E  Q +F P+G +MS+I++ ETPF
Sbjct: 372 TRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPF 431

Query: 430 SHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGS 489
            HR G +Y+I Y+  W+G     K +++ W+  +Y+ M+ FV+KSPR AY N RDLD+G 
Sbjct: 432 PHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM 491

Query: 490 NNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
                ++ Y++   WG+KYF NNF+RLV VKT VDP DFF  EQSIP
Sbjct: 492 YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210210 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 2.1e-150
Identity = 266/512 (51.95%), Postives = 359/512 (70.12%), Query Frame = 0

Query: 33  QNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVS 92
           +NF++C S++   +   +K+VYT  +  Y S+LN +I+NLRF+   TPKPL+IITP +VS
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYHPFIIVDLINLRSISIDIVNNTAWVES 152
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF+IVDL N+ S+ ID+ + TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVD 212
           GA+LG++YY I E +  L+FPAG CPTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPVPAMVTICSTIKNLE-DDTVK 272
            +G V DR+SMGEDLFWAIRGG G +FGI+ AWK++LV VP+M TI S  KN+E  + VK
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 VIHQWQYVANKLHEDIFLGI-VLTGGNTSTQGGKKNPIATFY-SLFLGKVDELVATLSTT 332
           ++++WQ +A    +++ L    +T   T  QG  K  I +++ S+F G VD LV  ++ +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCLEVRWVESTLIIASGVFQ---TVESLEPLLNRTPTTLESTKIKSDYIKE 392
           FPELG+ K DC ++ W++ T+I  SGV     T    E LL+R+     +  IK DY+K+
Sbjct: 332 FPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 393 PIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESETPFSHRAGYLYQIGYMVGWK 452
           PIP+ A+  I ++L   D+    FVF PYG  M +ISES  PF HRAG +Y+I Y+  W+
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWE 451

Query: 453 GQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGSNNKYGKTSYKQASIWGL 512
            Q  +   +HI+WIR +Y +  P+VS++PR AY NYRDLD+G  N     +Y QA IWG 
Sbjct: 452 KQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 513 KYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           KYFG NF RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Chy12G210210 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.8e-150
Identity = 266/532 (50.00%), Postives = 374/532 (70.30%), Query Frame = 0

Query: 10  LFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSI 69
           LF +L+  L+ SP  VSA    H++F++CLS   + +    KV++T  +SS+ S+L+ SI
Sbjct: 7   LFTILLFSLNISP-LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 66

Query: 70  RNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYH-PF 129
           +N RFS  ETPKP+ IITP   S +Q  + C++ HG+ +RTRS GH YEGLSY+AY+ PF
Sbjct: 67  QNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPF 126

Query: 130 IIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSG 189
            ++DL NLRSIS+D+ N T WV++GA+ G+LYY IG+ +++LAFPAGI PTVGVGG FSG
Sbjct: 127 AVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSG 186

Query: 190 GGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVK 249
           GGYG LLRKYGLAADN+IDA +VDA+G + DR++MGED FWAIRGG G SFG++++WKVK
Sbjct: 187 GGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVK 246

Query: 250 LVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGKKNPI 309
           LV VP+ +T+    K  + + V++I +WQY A+K+ +D+F+        T+ +   KN +
Sbjct: 247 LVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAV 306

Query: 310 -ATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTVESLEPLL 369
            A F  L++G V+ L+A +   FPELGL K+ C E+ W+ES L  A   F   ESL  L 
Sbjct: 307 HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVLT 366

Query: 370 NRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESET 429
           NR  T+L S K K D+++EPIP+AAI+ IW+RL+A +    + +  P+G +MS+++E ET
Sbjct: 367 NRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 426

Query: 430 PFSHRAGYLYQIGYMVGWKGQNLKAK---NRHISWIRELYEYMAPFVSKSPRAAYANYRD 489
           PF HR G LY+I Y+  W+ +  K K   ++++ W+  +YE+M P+VSKSPR AY N++D
Sbjct: 427 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 486

Query: 490 LDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
           +D+G      KT Y++   WG+KYF NNF+RLV VKT+VDP DFF  EQSIP
Sbjct: 487 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Chy12G210210 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 6.2e-150
Identity = 266/512 (51.95%), Postives = 358/512 (69.92%), Query Frame = 0

Query: 33  QNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVS 92
           +NF++CLS++   +   +K+VYT  +  Y S+LN +++NLRF+   TPKPL+I TP +VS
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYHPFIIVDLINLRSISIDIVNNTAWVES 152
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF+IVDL N+ S+ ID+ + TAWVES
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVD 212
           GA+LG++YY I E +  L+FPAG CPTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPVPAMVTICSTIKNLE-DDTVK 272
            +G V DR+SMGEDLFWAIRGG G +FGI+ AWK++LV VP+M TI S  KN+E  + VK
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 VIHQWQYVANKLHEDIFLGI-VLTGGNTSTQGGKKNPIATFY-SLFLGKVDELVATLSTT 332
           ++++WQ +A    +++ L    +T   T  QG  K  I +++ S+F G VD LV  ++ +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCLEVRWVESTLIIASGVFQ---TVESLEPLLNRTPTTLESTKIKSDYIKE 392
           FPELG+ K DC ++ W++ T+I  SGV     T    E LL+R+     +  IK DY+K+
Sbjct: 332 FPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 393 PIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESETPFSHRAGYLYQIGYMVGWK 452
           PIP+ A+  I ++L   D+    FVF PYG  M +ISES  PF HRAG  Y+I Y+  W+
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWE 451

Query: 453 GQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGSNNKYGKTSYKQASIWGL 512
            Q  +   +HI+WIR +Y +  P+VS++PR AY NYRDLD+G  N     +Y QA IWG 
Sbjct: 452 KQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 513 KYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           KYFG NF RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Chy12G210210 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.8e-149
Identity = 264/511 (51.66%), Postives = 352/511 (68.88%), Query Frame = 0

Query: 33  QNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVS 92
           +NF++C S+H   +    K+VYT  +  Y S+LN +I+NLRF    TPKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 93  HIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYHPFIIVDLINLRSISIDIVNNTAWVES 152
           HIQA ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+ SI ID+ + TAWVE+
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 153 GASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVD 212
           GA+LG++YY I EK+  L+FP G CPTVGVGGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPVPAMVTICSTIKNLE-DDTVK 272
            +G V DR+SMGEDLFWAIRGG G +FGI+ AWK+KLV VP+  TI S  KN+E    VK
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 273 VIHQWQYVANKLHEDIFLGI-VLTGGNTSTQGGKKNPI-ATFYSLFLGKVDELVATLSTT 332
           + ++WQ +A K  +D+ L    +T   T   G  K  +   F S+F G VD LV  ++ +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCLEVRWVESTLIIASGV-FQTVE-SLEPLLNRTPTTLESTKIKSDYIKEP 392
           FPELG+ K DC E  W+++T+  +  V F T     E LL+R+     +  IK DY+K+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 393 IPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESETPFSHRAGYLYQIGYMVGWKG 452
           IP+ A+  I ++L   D+    +V  PYG  M +ISES  PF HRAG +Y++ Y   W+ 
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 453 QNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGSNNKYGKTSYKQASIWGLK 512
           Q  +   +HI+W+R +Y +  P+VS++PR AY NYRDLD+G  N     +Y QA IWG K
Sbjct: 452 Q--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 513 YFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           YFG NF RLV VKTKVDP++FFR+EQSIP L
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Chy12G210210 vs. ExPASy TrEMBL
Match: A0A1S3BDB7 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.0e-293
Identity = 497/539 (92.21%), Postives = 514/539 (95.36%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLII-VLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINS 60
           MKSSSQL PLF VLII V+S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVVYTPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGA+LG+LYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM HDRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE D VKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGG K P ATFYSLFLGKVDELVATLSTTFPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 VESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           VESL+PLLNRTP+T++STKIKSDYIK+PIPKAAIEGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQISESETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of Chy12G210210 vs. ExPASy TrEMBL
Match: A0A5D3C1B2 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00230 PE=3 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.6e-292
Identity = 496/539 (92.02%), Postives = 513/539 (95.18%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLII-VLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINS 60
           MKSSSQL PLF VLII V+S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVVYTPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGA+LG+LYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM HDRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE D VKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGG K P ATFYSLFLGKVDELVATLSTTFPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 VESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           VESL+PLLNRTP+T++STKIKSDYIK+PIPKAA EGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAANEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQISESETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of Chy12G210210 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.2e-258
Identity = 440/538 (81.78%), Postives = 483/538 (89.78%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60
           MK SS LIPL LV I+V SAS    SA P K+QNF+QCLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKFSSTLIPLSLVFIVVSSAS-LCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTV 180
           SYVAY PFI+VDLINLRS+ +++ NNTAWVESGA+LG+LYYRIGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240
           G+GGHFSGGGYG +LRK+GLAADNVIDAYLVDANG V D+ESMGEDLFWAIRGG GGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWK+KLVPVP  VTICST +NLE+D +K+IH+WQYV NKL E+I+LGI+LTGGN ST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTV 360
           Q G  NP A F+SLFLG+ DELV TLST FPELGLIKQDC+E  WVESTLII  GV Q +
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGV-QPI 360

Query: 361 ESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMS 420
           E+LEPLLNRT TTL+STKIKSDYI EPIP AAI+ IWQRLK +DIE  Q VF PYG RMS
Sbjct: 361 ETLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMS 420

Query: 421 QISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYA 480
           QISESETPFSHRA YL++IGY+VGWK Q+LKAK RHISW+RE+YEYMAPFVSKSPRAAY 
Sbjct: 421 QISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYT 480

Query: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           NYRDLDIGSNNKYGKTSYK+AS+WGLKYFGNNF RLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 NYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210210 vs. ExPASy TrEMBL
Match: A0A1S3BDA8 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.2e-258
Identity = 440/538 (81.78%), Postives = 483/538 (89.78%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60
           MK SS LIPL LV I+V SAS    SA P K+QNF+QCLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKFSSTLIPLSLVFIVVSSAS-LCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTV 180
           SYVAY PFI+VDLINLRS+ +++ NNTAWVESGA+LG+LYYRIGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240
           G+GGHFSGGGYG +LRK+GLAADNVIDAYLVDANG V D+ESMGEDLFWAIRGG GGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWK+KLVPVP  VTICST +NLE+D +K+IH+WQYV NKL E+I+LGI+LTGGN ST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTV 360
           Q G  NP A F+SLFLG+ DELV TLST FPELGLIKQDC+E  WVESTLII  GV Q +
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGV-QPI 360

Query: 361 ESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMS 420
           E+LEPLLNRT TTL+STKIKSDYI EPIP AAI+ IWQRLK +DIE  Q VF PYG RMS
Sbjct: 361 ETLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMS 420

Query: 421 QISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYA 480
           QISESETPFSHRA YL++IGY+VGWK Q+LKAK RHISW+RE+YEYMAPFVSKSPRAAY 
Sbjct: 421 QISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYT 480

Query: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           NYRDLDIGSNNKYGKTSYK+AS+WGLKYFGNNF RLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 NYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210210 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.3e-256
Identity = 438/539 (81.26%), Postives = 483/539 (89.61%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60
           MK SS LIP  L+LI+V S+S SWVSA PHKHQNF+QCLSEHSSK YPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLILILV-SSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTV 180
           SYVAYHPFIIVDLINLRSI ID+  NTAWVESGA+LG+LYYRIGEKSRTLAFP G+CPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240
           GVGGHFSGGGYG +LRK+GLAADNVIDAYLVDANG VHDR+SMGEDLFWAIRGG GGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGN-TS 300
           IVVAWK+KLV VPA VTICST +NL+ D +K++HQWQYVANKL E++FLGI+LTGGN T+
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQ G  NPIATF+SLFLGKVD LVATLS  FPELG  K+DC+E  W+ESTLI A+GV  T
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGV-GT 360

Query: 361 VESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           VESLEPLLNRTP+ LE+ K+KSDY KEPI  A IEG+WQRLKA+DIE  Q +F PYG RM
Sbjct: 361 VESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRM 420

Query: 421 SQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQISE ETPFSHRAG LY+IGY V WK Q+LKAK +HISWIR++Y+YM PFVSKSPR+AY
Sbjct: 421 SQISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           ANYRDLDIG+N KYGKTS +QASIWGLKYFGNNFKRLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 ANYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Chy12G210210 vs. NCBI nr
Match: XP_011659198.2 (berberine bridge enzyme-like 18 [Cucumis sativus])

HSP 1 Score: 1058 bits (2735), Expect = 0.0
Identity = 527/542 (97.23%), Postives = 533/542 (98.34%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60
           MKSSSQLIPLFLVL IVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120
           YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHDYEGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTV 180
           SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGA+LG+LYYRIGEKSRTLAFPAGICPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240
           GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG
Sbjct: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240

Query: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWKVKLVPVPAMVTICSTIKNLEDD VKVIHQWQYVANKLHEDIFLGIVLTGGNTST
Sbjct: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300

Query: 301 QGGKKNPI----ATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGV 360
           QGG KNPI    ATFYSLFLGKVDELVATLST FPELGLIKQDCLEVRWVESTLIIASGV
Sbjct: 301 QGGIKNPIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGV 360

Query: 361 FQTVESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYG 420
           FQT+ESLEPLLNRTPTTL+STKIKSDYIK+PIPKAAIEGIWQRLKARDIEGPQFVFAPYG
Sbjct: 361 FQTIESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYG 420

Query: 421 ARMSQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPR 480
           ARMSQIS+SETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPR
Sbjct: 421 ARMSQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPR 480

Query: 481 AAYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 538
           AAYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP
Sbjct: 481 AAYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 540

BLAST of Chy12G210210 vs. NCBI nr
Match: XP_008445695.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1009 bits (2608), Expect = 0.0
Identity = 497/539 (92.21%), Postives = 514/539 (95.36%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVL-SASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINS 60
           MKSSSQL PLF VLIIV+ S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVVYTPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGA+LG+LYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM HDRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE D VKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGG K P ATFYSLFLGKVDELVATLSTTFPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 VESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           VESL+PLLNRTP+T++STKIKSDYIK+PIPKAAIEGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQISESETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 538
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of Chy12G210210 vs. NCBI nr
Match: TYK05058.1 (tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1006 bits (2601), Expect = 0.0
Identity = 496/539 (92.02%), Postives = 513/539 (95.18%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVL-SASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINS 60
           MKSSSQL PLF VLIIV+ S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVVYTPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGA+LG+LYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM HDRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE D VKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGG K P ATFYSLFLGKVDELVATLSTTFPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 VESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           VESL+PLLNRTP+T++STKIKSDYIK+PIPKAA EGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAANEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQISESETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 538
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of Chy12G210210 vs. NCBI nr
Match: XP_004151192.3 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical protein Csa_023212 [Cucumis sativus])

HSP 1 Score: 907 bits (2345), Expect = 0.0
Identity = 448/539 (83.12%), Postives = 491/539 (91.09%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60
           MK SS LIP  L+LI+V S+S  WVSA PHKH NF+QCLSEHSSKSYPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLILIVV-SSSSLWVSASPHKHHNFLQCLSEHSSKSYPISKVVHTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120
           YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTV 180
           SYVA HPFII+DLINLRS+ ID+ +NTAWVESG +LG+LYYRIGEKSRTLAFPAGICPTV
Sbjct: 121 SYVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240
           GVGGHFSGGGYG +LRK+GLAADNVIDAYLVDANG VHDRESMGEDLFWAIRGG GGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGN-TS 300
           IVVAWK+KLV VPA VT+C+T +NL+ D +K++HQWQYVANKL E++FLGI+LTGGN T+
Sbjct: 241 IVVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITT 300

Query: 301 TQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQ G  NPIATF+SLFLGKV+ELVATLSTTFPELGLIKQDC+E  W+ESTLI ++GV QT
Sbjct: 301 TQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGV-QT 360

Query: 361 VESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           VESLEPLLNRTP+ LE+ KIKSDYIKEPI  A IEGIWQRLKA+DIE  Q +F PYG RM
Sbjct: 361 VESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRM 420

Query: 421 SQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQISESETPFSHR G LY+IGY++GWK Q+LKAK +HISWIRE+YEYM PFVSKSPR+AY
Sbjct: 421 SQISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 538
           ANYRDLDIG N KYGKTS KQASIWGLKYFGNNFKRLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 ANYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Chy12G210210 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 439/538 (81.60%), Postives = 495/538 (92.01%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60
           MK SS LIPL LVLI++ S  P W +A P K+Q+F+QCLSEHSS SYPISKVVY+PINSS
Sbjct: 1   MKFSSPLIPLALVLIVLYSC-PLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120
           YSSVL+FSI+NLRFSKP+TPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTV 180
           SYVAY PFI+VDLINLRSIS+D  NNTAWVESGA+LG+LYYRIGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240
           G+GGHFSGGGYG +LRK+GLAADNVIDA+LVDANG VHDRESMGEDLFWAIRGG GGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWK+KLVPVPA VTICST +NLE+D +K++H+WQYVANKL E+++LGI+LTGG  ST
Sbjct: 241 IVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKAST 300

Query: 301 QGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTV 360
           QGG  NP ATF+SLFLG++DEL+ATLSTTFPELGLIKQDC+E  WVESTLIIA+GV Q +
Sbjct: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGV-QPI 360

Query: 361 ESLEPLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMS 420
           ESLEPLLNRTP T+E+TKIKSDY+KEP+P+AAIEGI QRLKA+DIE  Q +F PYG +MS
Sbjct: 361 ESLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMS 420

Query: 421 QISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYA 480
           QISESETPFSHRAG LY+IGY+VGWK Q+LKAK RHI WIRE+YEYM PFVSKSPRAAYA
Sbjct: 421 QISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 538
           NYRDLDIG+NNKYG+TS +QAS+WGLKYFGNNFK+LVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210210 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.5e-151
Identity = 262/527 (49.72%), Postives = 367/527 (69.64%), Query Frame = 0

Query: 10  LFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSI 69
           +F     +L + P  VSA      +F+QCLS   + S  +SKV++TP ++S+SSVL  SI
Sbjct: 12  IFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSI 71

Query: 70  RNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYHPFI 129
           +N RFS P+ PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHDYEGLSYV + PF+
Sbjct: 72  QNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFV 131

Query: 130 IVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSGG 189
           I+DL NLRSI++D+ N + WV++GA++G+LYY IG+K+RTLAFPAG+CPTVGVGGHFSGG
Sbjct: 132 ILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGG 191

Query: 190 GYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKL 249
           GYG LLRK+GLAAD+VIDA +VDA G + +R  MGED FWAIRGG G SF +V++WK+ L
Sbjct: 192 GYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGL 251

Query: 250 VPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGKKNPIA 309
           + VP+ VT+ +  K  E   +K+IH+WQ+VA+K+ +D+F+ ++L       Q  K    A
Sbjct: 252 INVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYKNMVRA 311

Query: 310 TFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTVESLEPLLNR 369
           +F  L+LG V  L+  ++  FPELGL + DC E+ W+ES +  A       E +  L  R
Sbjct: 312 SFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE---LGEEPINVLTKR 371

Query: 370 TPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESETPF 429
           T  +L + K KSD+++EP+PK AI  +W+RL+  + E  Q +F P+G +MS+I++ ETPF
Sbjct: 372 TRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPF 431

Query: 430 SHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGS 489
            HR G +Y+I Y+  W+G     K +++ W+  +Y+ M+ FV+KSPR AY N RDLD+G 
Sbjct: 432 PHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM 491

Query: 490 NNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
                ++ Y++   WG+KYF NNF+RLV VKT VDP DFF  EQSIP
Sbjct: 492 YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210210 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 533.5 bits (1373), Expect = 2.0e-151
Identity = 266/532 (50.00%), Postives = 374/532 (70.30%), Query Frame = 0

Query: 10  LFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSI 69
           LF +L+  L+ SP  VSA    H++F++CLS   + +    KV++T  +SS+ S+L+ SI
Sbjct: 7   LFTILLFSLNISP-LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 66

Query: 70  RNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYH-PF 129
           +N RFS  ETPKP+ IITP   S +Q  + C++ HG+ +RTRS GH YEGLSY+AY+ PF
Sbjct: 67  QNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPF 126

Query: 130 IIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSG 189
            ++DL NLRSIS+D+ N T WV++GA+ G+LYY IG+ +++LAFPAGI PTVGVGG FSG
Sbjct: 127 AVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSG 186

Query: 190 GGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVK 249
           GGYG LLRKYGLAADN+IDA +VDA+G + DR++MGED FWAIRGG G SFG++++WKVK
Sbjct: 187 GGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVK 246

Query: 250 LVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGKKNPI 309
           LV VP+ +T+    K  + + V++I +WQY A+K+ +D+F+        T+ +   KN +
Sbjct: 247 LVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAV 306

Query: 310 -ATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTVESLEPLL 369
            A F  L++G V+ L+A +   FPELGL K+ C E+ W+ES L  A   F   ESL  L 
Sbjct: 307 HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVLT 366

Query: 370 NRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESET 429
           NR  T+L S K K D+++EPIP+AAI+ IW+RL+A +    + +  P+G +MS+++E ET
Sbjct: 367 NRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 426

Query: 430 PFSHRAGYLYQIGYMVGWKGQNLKAK---NRHISWIRELYEYMAPFVSKSPRAAYANYRD 489
           PF HR G LY+I Y+  W+ +  K K   ++++ W+  +YE+M P+VSKSPR AY N++D
Sbjct: 427 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 486

Query: 490 LDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
           +D+G      KT Y++   WG+KYF NNF+RLV VKT+VDP DFF  EQSIP
Sbjct: 487 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Chy12G210210 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.7e-147
Identity = 259/533 (48.59%), Postives = 367/533 (68.86%), Query Frame = 0

Query: 11  FLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSIR 70
           FL+L + LS  PS +SA    H+NF++CLS   ++    S++++T  + SY S+LN SI+
Sbjct: 14  FLLLNLSLSHFPS-ISAQRTNHENFLKCLSHRINED--DSRIIHTSKDPSYFSILNSSIQ 73

Query: 71  NLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVA-YHPFI 130
           N RF   ETPKP+ IITP   + +Q+ + C++ HG+ IRTRSGGHDYEGLSY+A   PF+
Sbjct: 74  NPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFV 133

Query: 131 IVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTVGVGGHFSGG 190
           ++DL NLRSI++D+ N T WV+SGA++G+LYY IG+ S++LAFPAG+ PTVG+GG F GG
Sbjct: 134 VIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGG 193

Query: 191 GYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKL 250
           GYG L+RKYGL+ADNVIDA++VDANG   DR+ MGED FWAIRGG G SF +V++WK++L
Sbjct: 194 GYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRL 253

Query: 251 VPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGKKNPIA 310
           + VP++VT+   +K  E + V +I++WQY+A+K+  D+F+  +L          +    A
Sbjct: 254 LDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ--------KETEVYA 313

Query: 311 TFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTVESLEPLLNR 370
           +F  L+LG V +L+A +   FPELGL   +C E+ W+ES L    G     ES+E L  R
Sbjct: 314 SFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKG-----ESMEILAKR 373

Query: 371 TPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISESETPF 430
             T+  S K K D+I+EPIPK AI+ +W+R +A +    + +  P+G +MS+I+++E PF
Sbjct: 374 KRTS-RSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPF 433

Query: 431 SHRAGYLYQIGYMVGWKGQNLKAK---NRHISWIRELYEYMAPFVSKSPRAAYANYRDLD 490
            HR G LY+I Y+  W  +  K K    +++ W+  +YE+M P+VSKSPR AY N+RD+D
Sbjct: 434 PHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDID 493

Query: 491 IGSNNKYG-KTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           +G       KT Y++A +WG+KYF NNF RLV VKT VDP DFF  EQSIP +
Sbjct: 494 LGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of Chy12G210210 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 520.0 bits (1338), Expect = 2.3e-147
Identity = 257/541 (47.50%), Postives = 365/541 (67.47%), Query Frame = 0

Query: 5   SQLIPLFLVLIIVLSA-SPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPIN--SSY 64
           S+ +PLF +L++  S  + +  S+       F+ C+  ++   +P+ K  + P    S +
Sbjct: 4   SKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMF 63

Query: 65  SSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLS 124
           S VL  + +NLRF K   PKP  I +P H SH+QA++ICSK   + +R RSGGHDYEGLS
Sbjct: 64  SQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 123

Query: 125 YVAY--HPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPT 184
           YV+    PFI++DL  +R ++I+I +N+AWV+SGA++G+LYYRI EKS+   FPAG+C +
Sbjct: 124 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 183

Query: 185 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSF 244
           +G+GGH +GG YG ++RKYGL ADNV+DA +VDANG + DR +MGED FWAIRGG+GGSF
Sbjct: 184 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 243

Query: 245 GIVVAWKVKLVPVPAMVTICSTIKNLEDDT-VKVIHQWQYVANKLHEDIFLGIVLTGGNT 304
           GI++AWK+KLVPVP  VT+ +  K L+ D   K+I +WQ VA+KL E++F+ ++    N 
Sbjct: 244 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NV 303

Query: 305 STQGGKKNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQ 364
           +  GG K    ++ +LFLG    L+  +  +FPELGL  +DC+E+ W+ES   I+   F 
Sbjct: 304 AGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG--FP 363

Query: 365 TVESLEPLL-NRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGA 424
           T      LL  ++P    S K KSD++K PIP++ ++GI+++L   DI  P  ++ PYG 
Sbjct: 364 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGG 423

Query: 425 RMSQISESETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRA 484
            M++I ES+ PF HR G L+++ Y+  W   + K  +RHI+WIR+LY YM P+VS +PR 
Sbjct: 424 MMAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPRE 483

Query: 485 AYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPT 539
           AY NYRDLD+G N K  KT  KQA +WG  YF NNF RL+ +K KVDP +FFRHEQSIP 
Sbjct: 484 AYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 536

BLAST of Chy12G210210 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 512.7 bits (1319), Expect = 3.6e-145
Identity = 259/535 (48.41%), Postives = 358/535 (66.92%), Query Frame = 0

Query: 7   LIPLFLVLIIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLN 66
           ++ LF V+ I  S+S S  S      + F QCL+ +S   +PIS  ++   N SYSSVL 
Sbjct: 6   ILVLFFVVFIWQSSSSSANS------ETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQ 65

Query: 67  FSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAY- 126
            +IRNLRF+   TPKP LII  +H SH+QAA+ C K H LQ++ RSGGHDY+GLSYV Y 
Sbjct: 66  ANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYS 125

Query: 127 -HPFIIVDLINLRSISIDIVNNTAWVESGASLGQLYYRIGEKSRTLAFPAGICPTVGVGG 186
             PF ++D+ NLRS+ +D+ + TAWV++GA LG++YY I EKS+TLA+PAGICPTVGVGG
Sbjct: 126 GKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGG 185

Query: 187 HFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVA 246
           H SGGGYG ++RKYGL  DN IDA +VD NG + DR+ MGEDL+WAI GG GGS+G+V+A
Sbjct: 186 HISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLA 245

Query: 247 WKVKLVPVPAMVTICSTIKNLEDDTVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGK 306
           +K+ LV VP  VT+    + LE +   +IH+WQ VA KL +++F+  V+   N  T   +
Sbjct: 246 YKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVN-GTVSSQ 305

Query: 307 KNPIATFYSLFLGKVDELVATLSTTFPELGLIKQDCLEVRWVESTLIIASGVFQTVESLE 366
           K    TF ++FLG    L++ L+  FPELGL++ DC E  W++S L   +     V S E
Sbjct: 306 KTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN---IQVGSSE 365

Query: 367 PLLNRTPTTLESTKIKSDYIKEPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISE 426
            LL +    +   K KSDY++EPI +  +E IW+  K  ++E P   F PYG  M +IS 
Sbjct: 366 TLLLQRNQPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISS 425

Query: 427 SETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRD 486
           + TPF +RAG L++I Y   W+ + L   +R++   R+LY++M PFVSK+PR ++ NYRD
Sbjct: 426 TVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRD 485

Query: 487 LDIGSNNKYGK-TSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           +D+G N+  GK +SY +   +G KYF  NF+RLV +KT+VD  +FFR+EQSIP L
Sbjct: 486 VDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SVG52.1e-15049.72Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
A6P6W02.1e-15051.95Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=... [more]
Q9FI212.8e-15050.00Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... [more]
A6P6W16.2e-15051.95Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=... [more]
Q8GTB61.8e-14951.66Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A1S3BDB74.0e-29392.21tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348865... [more]
A0A5D3C1B22.6e-29292.02Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A5A7TU574.2e-25881.78Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BDA84.2e-25881.78tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
A0A5D3C1A11.3e-25681.26Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
XP_011659198.20.097.23berberine bridge enzyme-like 18 [Cucumis sativus][more]
XP_008445695.10.092.21PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo][more]
TYK05058.10.092.02tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa][more]
XP_004151192.30.083.12berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical pro... [more]
XP_038895543.10.081.60berberine bridge enzyme-like 18 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G20820.11.5e-15149.72FAD-binding Berberine family protein [more]
AT5G44440.12.0e-15150.00FAD-binding Berberine family protein [more]
AT5G44410.11.7e-14748.59FAD-binding Berberine family protein [more]
AT5G44400.12.3e-14747.50FAD-binding Berberine family protein [more]
AT1G30700.13.6e-14548.41FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 30..136
e-value: 4.8E-46
score: 157.0
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 478..536
e-value: 1.0E-19
score: 70.3
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 82..218
e-value: 1.4E-26
score: 92.9
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 140..536
e-value: 4.4E-157
score: 525.3
NoneNo IPR availableGENE3D3.40.462.20coord: 255..474
e-value: 4.4E-157
score: 525.3
NoneNo IPR availablePANTHERPTHR32448:SF142CANNABIDIOLIC ACID SYNTHASE-LIKEcoord: 5..538
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 5..538
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 78..252
score: 20.177185
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 29..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G210210.1Chy12G210210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding