Chy12G210200 (gene) Cucumber (hystrix) v1

BLAST of Chy12G210200 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.1e-153
Identity = 269/534 (50.37%), Postives = 379/534 (70.97%), Query Frame = 0

Query: 3   FSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYS 62
           FSS LI  +   +++  P  + A+ S Q   +FL CLS   + S  +SKV++TP ++S+S
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQ--SSFLQCLSLQLNDSNIVSKVIHTPNDTSFS 66

Query: 63  SVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSY 122
           SVL  SI+N  FS P++PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLSY
Sbjct: 67  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 126

Query: 123 VAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGI 182
           V ++PF+++DL NLRS+ VDV+N + WV++GAT+GELYY IG+K+RTLAFPAGVCPTVG+
Sbjct: 127 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 186

Query: 183 GGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFGIV 242
           GGHFSGGGYG +LRK GLAAD+VIDA +VDA G++L+R  MGEDFFWAIRGGGG SF +V
Sbjct: 187 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 246

Query: 243 VAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNASTQA 302
           ++WKI L+ VP TVT+ +  +  E+ A+K+IHRWQ+V +K+ +++++ V+L       Q 
Sbjct: 247 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QR 306

Query: 303 GITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTL-IIPIGVQPIES 362
                 A F  L+LG V  L+  ++  FPELGL + DC E SW+ES +    +G +PI  
Sbjct: 307 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPI-- 366

Query: 363 LEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQI 422
              L  RT  +L + K KSD+V+EP+P+  I  +W+RL+  + E +Q+IF P+GG+MS+I
Sbjct: 367 -NVLTKRTRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 426

Query: 423 SESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTNY 482
           ++ ETPFPHR G +++I Y+  W+      K++++ W+  VY+ M+ FV+KSPR AY N 
Sbjct: 427 ADYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINL 486

Query: 483 RDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIP 536
           RDLD+G      ++ Y+    WG+KYF +NF+RLV VKT VDP DFF  EQSIP
Sbjct: 487 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210200 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 6.6e-152
Identity = 267/542 (49.26%), Postives = 377/542 (69.56%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           M+FSS L  + L+F + ISP     SA    +++FL CLS   + +    KV++T  +SS
Sbjct: 1   MEFSSFLFTI-LLFSLNISP---LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           + S+LD SI+N  FS  E PKP+ IITP   S +Q  + C++ HG+ +RTRS GH +EGL
Sbjct: 61  FFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGL 120

Query: 121 SYVAY-RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPT 180
           SY+AY +PF V+DL NLRS+ +DV+N T WV++GAT GELYY IG+ +++LAFPAG+ PT
Sbjct: 121 SYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPT 180

Query: 181 VGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSF 240
           VG+GG FSGGGYG +LRK+GLAADN+IDA +VDA G++LDR++MGED+FWAIRGGGG SF
Sbjct: 181 VGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSF 240

Query: 241 GIVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNAS 300
           G++++WK+KLV VP T+T+    +  +++A+++I +WQY  +K+ +++++   L   N +
Sbjct: 241 GVILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTL---IIPIGV 360
                    A F  L++G V+ L+A +   FPELGL K+ C E SW+ES L     P G 
Sbjct: 301 A------VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG- 360

Query: 361 QPIESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGG 420
              ESL  L NR  T+L S K K D+V+EPIPEA I+ IW+RL+A +    ++I  P+GG
Sbjct: 361 ---ESLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGG 420

Query: 421 RMSQISESETPFPHRAGYLFKIGYVVGWKDQSLKAK---KRHISWIREVYEYMAPFVSKS 480
           +MS+++E ETPFPHR G L++I YV  W+++  K K    +++ W+  VYE+M P+VSKS
Sbjct: 421 KMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKS 480

Query: 481 PRAAYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQS 536
           PR AY N++D+D+G      KT Y+    WG+KYF +NF+RLV VKT+VDP DFF  EQS
Sbjct: 481 PRGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQS 527

BLAST of Chy12G210200 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.3e-151
Identity = 262/512 (51.17%), Postives = 350/512 (68.36%), Query Frame = 0

Query: 33  QNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPEIPKPLLIITPSHVS 92
           +NFL C S+H   +    K+VYT  +  Y S+L+ +I+NL F     PKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 93  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENNTAWVES 152
           HIQA ++CSK  GLQIRTRSGGHD EG+SY++  PF+VVDL N+ S+K+DV + TAWVE+
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVD 212
           GATLGE+YY I EK+  L+FP G CPTVG+GGHFSGGGYG ++R +GLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 AHGKVLDRESMGEDFFWAIRGGGGGSFGIVVAWKIKLVPVPPTVTICSTDRNLE-EDAIK 272
             GKVLDR+SMGED FWAIRGGGG +FGI+ AWKIKLV VP   TI S  +N+E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 273 LIHRWQYVGNKLDENVYLGVILTGGNASTQAGITNPTAR--FFSLFLGRVDELMATLSTT 332
           L ++WQ +  K D+++ L       N +   G    T    F S+F G VD L+  ++ +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCVEASWVESTLIIPIGVQPIESL----EPLLNRTPTNLDSTKIKSDYVKE 392
           FPELG+ K DC E SW+++T+    GV    +     E LL+R+     +  IK DYVK+
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYS-GVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKK 391

Query: 393 PIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIGYVVGWK 452
           PIPE  +  I ++L  +D+     +  PYGG M +ISES  PFPHRAG ++++ Y   W+
Sbjct: 392 PIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE 451

Query: 453 DQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKRASIWGM 512
            Q  +  ++HI+W+R VY +  P+VS++PR AY NYRDLD+G  N     +Y +A IWG 
Sbjct: 452 KQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGE 511

Query: 513 KYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Chy12G210200 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.7e-150
Identity = 264/518 (50.97%), Postives = 357/518 (68.92%), Query Frame = 0

Query: 27  ASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPEIPKPLLII 86
           A+PQ  +NFL C S++   +   +K+VYT  +  Y S+L+ +I+NL F+    PKPL+II
Sbjct: 28  ANPQ--ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVII 87

Query: 87  TPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENN 146
           TP +VSHIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+ SVK+DV + 
Sbjct: 88  TPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQ 147

Query: 147 TAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 206
           TAWVE+GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R +GLAADN+I
Sbjct: 148 TAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNII 207

Query: 207 DAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFGIVVAWKIKLVPVPPTVTICSTDRNLE 266
           DA+LV+  GKVLDR+SMGED FWAIRGGGG +FGI+ AWKI+LV VP   TI S  +N+E
Sbjct: 208 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNME 267

Query: 267 -EDAIKLIHRWQYVGNKLDENVYLGVILTGGNASTQAGITNPT--ARFFSLFLGRVDELM 326
             + +KL+++WQ +    ++ + L       N +   G    T  + F S+F G VD L+
Sbjct: 268 IHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLV 327

Query: 327 ATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESL----EPLLNRTPTNLDSTKIK 386
             ++ +FPELG+ K DC + SW++ T+I   GV    +     E LL+R+     +  IK
Sbjct: 328 DLMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIK 387

Query: 387 SDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIG 446
            DYVK+PIPE  +  I ++L  +D+     +F PYGG M +ISES  PFPHRAG +++I 
Sbjct: 388 LDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIW 447

Query: 447 YVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKR 506
           Y+  W+ Q  +  ++HI+WIR VY +  P+VS++PR AY NYRDLD+G  N     +Y +
Sbjct: 448 YIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQ 507

Query: 507 ASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           A IWG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Chy12G210200 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.1e-150
Identity = 264/518 (50.97%), Postives = 356/518 (68.73%), Query Frame = 0

Query: 27  ASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPEIPKPLLII 86
           A+PQ  +NFL CLS++   +   +K+VYT  +  Y S+L+ +++NL F+    PKPL+I 
Sbjct: 28  ANPQ--ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVIT 87

Query: 87  TPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENN 146
           TP +VSHIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+ SVK+DV + 
Sbjct: 88  TPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQ 147

Query: 147 TAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 206
           TAWVESGATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R +GLAADN+I
Sbjct: 148 TAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNII 207

Query: 207 DAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFGIVVAWKIKLVPVPPTVTICSTDRNLE 266
           DA+LV+  GKVLDR+SMGED FWAIRGGGG +FGI+ AWKI+LV VP   TI S  +N+E
Sbjct: 208 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNME 267

Query: 267 -EDAIKLIHRWQYVGNKLDENVYLGVILTGGNASTQAGITNPT--ARFFSLFLGRVDELM 326
             + +KL+++WQ +    ++ + L       N +   G    T  + F S+F G VD L+
Sbjct: 268 IHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLV 327

Query: 327 ATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESL----EPLLNRTPTNLDSTKIK 386
             ++ +FPELG+ K DC + SW++ T+I   GV    +     E LL+R+     +  IK
Sbjct: 328 DLMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIK 387

Query: 387 SDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIG 446
            DYVK+PIPE  +  I ++L  +D+     +F PYGG M +ISES  PFPHRAG  ++I 
Sbjct: 388 LDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIW 447

Query: 447 YVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKR 506
           Y+  W+ Q  +  ++HI+WIR VY +  P+VS++PR AY NYRDLD+G  N     +Y +
Sbjct: 448 YIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQ 507

Query: 507 ASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           A IWG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Chy12G210200 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.3e-295
Identity = 502/537 (93.48%), Postives = 519/537 (96.65%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MKFSSTLIPLSL+F IV+S ASLCASASPQKYQNFL CLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKFSSTLIPLSLVF-IVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAYRPFIVVDLINLRSVKV+VENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKVLD+ESMGED FWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNAST 300
           IVVAWKIKLVPVP TVTICSTDRNLEEDAIKLIHRWQYVGNKLDEN+YLG+ILTGGNAST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360
           QAGITNPTARFFSLFLGR DEL+ TLST FPELGLIKQDCVEASWVESTLIIPIGVQPIE
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360

Query: 361 SLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQ 420
           +LEPLLNRT T LDSTKIKSDY+ EPIP A I+ IWQRLK QDIETSQ++FVPYGGRMSQ
Sbjct: 361 TLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 420

Query: 421 ISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTN 480
           ISESETPF HRA YLFKIGYVVGWKDQSLKAKKRHISW+REVYEYMAPFVSKSPRAAYTN
Sbjct: 421 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 480

Query: 481 YRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           YRDLDIGSNNKYGKTSYKRAS+WG+KYFG+NFDRLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210200 vs. ExPASy TrEMBL
Match: A0A1S3BDA8 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.3e-295
Identity = 502/537 (93.48%), Postives = 519/537 (96.65%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MKFSSTLIPLSL+F IV+S ASLCASASPQKYQNFL CLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKFSSTLIPLSLVF-IVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAYRPFIVVDLINLRSVKV+VENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKVLD+ESMGED FWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNAST 300
           IVVAWKIKLVPVP TVTICSTDRNLEEDAIKLIHRWQYVGNKLDEN+YLG+ILTGGNAST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360
           QAGITNPTARFFSLFLGR DEL+ TLST FPELGLIKQDCVEASWVESTLIIPIGVQPIE
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360

Query: 361 SLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQ 420
           +LEPLLNRT T LDSTKIKSDY+ EPIP A I+ IWQRLK QDIETSQ++FVPYGGRMSQ
Sbjct: 361 TLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 420

Query: 421 ISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTN 480
           ISESETPF HRA YLFKIGYVVGWKDQSLKAKKRHISW+REVYEYMAPFVSKSPRAAYTN
Sbjct: 421 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 480

Query: 481 YRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           YRDLDIGSNNKYGKTSYKRAS+WG+KYFG+NFDRLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210200 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.8e-260
Identity = 439/538 (81.60%), Postives = 486/538 (90.33%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK+SS LIP SLI +I++S +S   SASP K+QNFL CLSEHSSK YPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLI-LILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAY PFI+VDLINLRS+K+DVE NTAWVESGATLGELYYRIGEKSRTLAFP GVCPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           G+GGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKV DR+SMGED FWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGN-AS 300
           IVVAWKIKLV VP TVTICST+RNL+ D IKL+H+WQYV NKLDEN++LG++LTGGN  +
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPI 360
           TQ GI NP A FFSLFLG+VD L+ATLS  FPELG  K+DCVE SW+ESTLI   GV  +
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTV 360

Query: 361 ESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMS 420
           ESLEPLLNRTP+NL++ K+KSDY KEPI  ATIEG+WQRLKA+DIETSQ+IF+PYGGRMS
Sbjct: 361 ESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYT 480
           QISE ETPF HRAG L+KIGY V WK+QSLKAKK+HISWIR++Y+YM PFVSKSPR+AY 
Sbjct: 421 QISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           NYRDLDIG+N KYGKTS ++ASIWG+KYFG+NF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Chy12G210200 vs. ExPASy TrEMBL
Match: A0A1S3BDZ5 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.8e-260
Identity = 439/538 (81.60%), Postives = 486/538 (90.33%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK+SS LIP SLI +I++S +S   SASP K+QNFL CLSEHSSK YPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLI-LILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAY PFI+VDLINLRS+K+DVE NTAWVESGATLGELYYRIGEKSRTLAFP GVCPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           G+GGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKV DR+SMGED FWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGN-AS 300
           IVVAWKIKLV VP TVTICST+RNL+ D IKL+H+WQYV NKLDEN++LG++LTGGN  +
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPI 360
           TQ GI NP A FFSLFLG+VD L+ATLS  FPELG  K+DCVE SW+ESTLI   GV  +
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTV 360

Query: 361 ESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMS 420
           ESLEPLLNRTP+NL++ K+KSDY KEPI  ATIEG+WQRLKA+DIETSQ+IF+PYGGRMS
Sbjct: 361 ESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYT 480
           QISE ETPF HRAG L+KIGY V WK+QSLKAKK+HISWIR++Y+YM PFVSKSPR+AY 
Sbjct: 421 QISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           NYRDLDIG+N KYGKTS ++ASIWG+KYFG+NF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Chy12G210200 vs. ExPASy TrEMBL
Match: A0A1S3BDB7 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.9e-259
Identity = 432/539 (80.15%), Postives = 483/539 (89.61%), Query Frame = 0

Query: 1   MKFSSTLIPL-SLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINS 60
           MK SS L PL  ++ I+V+S + L  SA P ++QNFLHCL EHSSKSYPISKVVYTPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 120
           SYSSVL+FSIRN  FSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD+EG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPT 180
           LSYVAYRPFI+VDLIN+RS+ VD+ NNTAWVESGATLGELYYRIGEKSRTLAFPAG+CPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSF 240
           VG+GGHFSGGGYG +LRK+GLAADNVIDAYLVDA+G   DRESMGED FWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNAS 300
           GIVVAWK+KLVPVP  VTICST +NLE DA+K+IH+WQYV NKL E+++LG++LTGGN S
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGV-QP 360
           TQ GI  PTA F+SLFLG+VDEL+ATLSTTFPE GLIK+DC+E  W+ESTLII  GV Q 
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 IESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRM 420
           +ESL+PLLNRTP+ +DSTKIKSDY+K+PIP+A IEGIWQRLKA DIE  Q++FVPYG RM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAY 480
           SQISESETPF HRAGYL+KIGY+VGWK Q+LKAK RHISW RE+YEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 TNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
            NYRDLDIGSNNKYGKTSYK+ASIWG+KYFG+NF RLVYVK KVDP+DFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of Chy12G210200 vs. NCBI nr
Match: XP_008445684.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047103.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05055.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1016 bits (2627), Expect = 0.0
Identity = 502/537 (93.48%), Postives = 519/537 (96.65%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MKFSSTLIPLSL+FI+V S ASLCASASPQKYQNFL CLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKFSSTLIPLSLVFIVV-SSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAYRPFIVVDLINLRSVKV+VENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKVLD+ESMGED FWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNAST 300
           IVVAWKIKLVPVP TVTICSTDRNLEEDAIKLIHRWQYVGNKLDEN+YLG+ILTGGNAST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360
           QAGITNPTARFFSLFLGR DEL+ TLST FPELGLIKQDCVEASWVESTLIIPIGVQPIE
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360

Query: 361 SLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQ 420
           +LEPLLNRT T LDSTKIKSDY+ EPIP A I+ IWQRLK QDIETSQ++FVPYGGRMSQ
Sbjct: 361 TLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 420

Query: 421 ISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTN 480
           ISESETPF HRA YLFKIGYVVGWKDQSLKAKKRHISW+REVYEYMAPFVSKSPRAAYTN
Sbjct: 421 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 480

Query: 481 YRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 537
           YRDLDIGSNNKYGKTSYKRAS+WG+KYFG+NFDRLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210200 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 469/537 (87.34%), Postives = 503/537 (93.67%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MKFSS LIPL+L+ I++ S   L A+ASPQKYQ+FL CLSEHSS SYPISKVVY+PINSS
Sbjct: 1   MKFSSPLIPLALVLIVLYS-CPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSI+NL FSKP+ PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAYRPFIVVDLINLRS+ VD ENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           GIGGHFSGGGYGLMLRKFGLAADNVIDA+LVDA+GKV DRESMGED FWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNAST 300
           IVVAWKIKLVPVP TVTICST RNLEEDAIKL+HRWQYV NKLDEN+YLG+ILTGG AST
Sbjct: 241 IVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKAST 300

Query: 301 QAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360
           Q GITNPTA FFSLFLGR+DEL+ATLSTTFPELGLIKQDCVEA+WVESTLII  GVQPIE
Sbjct: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIE 360

Query: 361 SLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQ 420
           SLEPLLNRTP  +++TKIKSDYVKEP+PEA IEGI QRLKAQDIETSQ+IFVPYGG+MSQ
Sbjct: 361 SLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQ 420

Query: 421 ISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTN 480
           ISESETPF HRAG L+KIGYVVGWK+QSLKAKKRHI WIREVYEYM PFVSKSPRAAY N
Sbjct: 421 ISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYAN 480

Query: 481 YRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 537
           YRDLDIG+NNKYG+TS ++AS+WG+KYFG+NF +LVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210200 vs. NCBI nr
Match: XP_004151192.3 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical protein Csa_023212 [Cucumis sativus])

HSP 1 Score: 915 bits (2366), Expect = 0.0
Identity = 447/538 (83.09%), Postives = 494/538 (91.82%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK+SS LIP SLI +IV+S +SL  SASP K+ NFL CLSEHSSKSYPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLI-LIVVSSSSLWVSASPHKHHNFLQCLSEHSSKSYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVL+FSIRNL FSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD+EGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVA  PFI++DLINLRS+K+DVE+NTAWVESG TLGELYYRIGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           G+GGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKV DRESMGED FWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGN-AS 300
           IVVAWKIKLV VP TVT+C+T RNL+ D IKL+H+WQYV NKLDEN++LG+ILTGGN  +
Sbjct: 241 IVVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITT 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPI 360
           TQ GITNP A FFSLFLG+V+EL+ATLSTTFPELGLIKQDC+E SW+ESTLI   GVQ +
Sbjct: 301 TQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTV 360

Query: 361 ESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMS 420
           ESLEPLLNRTP+NL++ KIKSDY+KEPI  ATIEGIWQRLKAQDIETSQ+IF+PYGGRMS
Sbjct: 361 ESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYT 480
           QISESETPF HR G L+KIGY++GWK+QSLKAKK+HISWIRE+YEYM PFVSKSPR+AY 
Sbjct: 421 QISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 537
           NYRDLDIG N KYGKTS K+ASIWG+KYFG+NF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Chy12G210200 vs. NCBI nr
Match: XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 446/538 (82.90%), Postives = 490/538 (91.08%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK++S LI LSL+ +IV+S + L ASAS QKYQ+FL CL EHS  +YPISKVVY+PINSS
Sbjct: 1   MKYTSPLILLSLV-LIVVSSSPLWASASEQKYQHFLQCLLEHSLDNYPISKVVYSPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNL FSKP+ PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAY PFI+VDLINLRSV +DVEN+TAWV+SGATLGELYYRIGEKSRTLAFPAGVCPTV
Sbjct: 121 SYVAYLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           G+GGHFSGGGYG MLRKFGLAADNVIDAYLVDA+GKV DRESMGED FWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGN-AS 300
           IVVAWK+KLVPVP TVT CST RNLE DAIKL+H+WQYV NKLDEN++LG++LTGGN  +
Sbjct: 241 IVVAWKVKLVPVPATVTSCSTHRNLEGDAIKLVHQWQYVANKLDENLFLGILLTGGNITT 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPI 360
           TQ GI +PTA FF LFLG+VDEL+ATLSTTFPEL L+KQDCVE SW+ESTLI   GVQ +
Sbjct: 301 TQEGIADPTATFFILFLGKVDELVATLSTTFPELSLMKQDCVETSWIESTLIAATGVQIV 360

Query: 361 ESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMS 420
           ESLEPLLNRTP  ++STKIKSDY+KEPIPEATIEGI+QRLKAQDI TSQ+IFVPYGGRMS
Sbjct: 361 ESLEPLLNRTPPTIESTKIKSDYIKEPIPEATIEGIFQRLKAQDIATSQLIFVPYGGRMS 420

Query: 421 QISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYT 480
           QISESETPF HRA  L+KIGY+VGWK+QSL AKKRHISWIREVYEYM PFVSKSPR+AY 
Sbjct: 421 QISESETPFSHRAANLYKIGYIVGWKEQSLNAKKRHISWIREVYEYMTPFVSKSPRSAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 537
           NYRDLDIG+NNKY KTS K+AS+WG+KYFG+NF RLVYVKTKVDPYDFFRHEQSIP L
Sbjct: 481 NYRDLDIGANNKYRKTSVKQASVWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPAL 537

BLAST of Chy12G210200 vs. NCBI nr
Match: XP_008445674.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >TYK05048.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 440/538 (81.78%), Postives = 485/538 (90.15%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK+SS LIP SLI I+V S +S   SASP K+QNFL CLSEHSSK YPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLILILVSSSSSW-VSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPE PKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           SYVAY PFI+VDLINLRS+K+DVE NTAWVESGATLGELYYRIGEKSRTLAFP GVCPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFG 240
           G+GGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKV DR+SMGED FWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGN-AS 300
           IVVAWKIKLV VP TVTICST+RNL+ D IKL+H+WQYV NKLDEN++LG++LTGGN  +
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPI 360
           TQ GI NP A FFSLFLG+VD L+ATLS  FPELG  K+DCVE SW+ESTLI   GV  +
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTV 360

Query: 361 ESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMS 420
           ESLEPLLNRTP+NL++ K+KSDY KEPI  ATIEG+WQRLKA+DIETSQ+IF+PYGGRMS
Sbjct: 361 ESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYT 480
           QISE ETPF HRAG L+KIGY V WK+QSLKAKK+HISWIR++Y+YM PFVSKSPR+AY 
Sbjct: 421 QISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 537
           NYRDLDIG+N KYGKTS ++ASIWG+KYFG+NF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Chy12G210200 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 543.9 bits (1400), Expect = 1.5e-154
Identity = 269/534 (50.37%), Postives = 379/534 (70.97%), Query Frame = 0

Query: 3   FSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYS 62
           FSS LI  +   +++  P  + A+ S Q   +FL CLS   + S  +SKV++TP ++S+S
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQ--SSFLQCLSLQLNDSNIVSKVIHTPNDTSFS 66

Query: 63  SVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSY 122
           SVL  SI+N  FS P++PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLSY
Sbjct: 67  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 126

Query: 123 VAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGI 182
           V ++PF+++DL NLRS+ VDV+N + WV++GAT+GELYY IG+K+RTLAFPAGVCPTVG+
Sbjct: 127 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 186

Query: 183 GGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSFGIV 242
           GGHFSGGGYG +LRK GLAAD+VIDA +VDA G++L+R  MGEDFFWAIRGGGG SF +V
Sbjct: 187 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 246

Query: 243 VAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNASTQA 302
           ++WKI L+ VP TVT+ +  +  E+ A+K+IHRWQ+V +K+ +++++ V+L       Q 
Sbjct: 247 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QR 306

Query: 303 GITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTL-IIPIGVQPIES 362
                 A F  L+LG V  L+  ++  FPELGL + DC E SW+ES +    +G +PI  
Sbjct: 307 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPI-- 366

Query: 363 LEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQI 422
              L  RT  +L + K KSD+V+EP+P+  I  +W+RL+  + E +Q+IF P+GG+MS+I
Sbjct: 367 -NVLTKRTRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 426

Query: 423 SESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRAAYTNY 482
           ++ ETPFPHR G +++I Y+  W+      K++++ W+  VY+ M+ FV+KSPR AY N 
Sbjct: 427 ADYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINL 486

Query: 483 RDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIP 536
           RDLD+G      ++ Y+    WG+KYF +NF+RLV VKT VDP DFF  EQSIP
Sbjct: 487 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210200 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 538.9 bits (1387), Expect = 4.7e-153
Identity = 267/542 (49.26%), Postives = 377/542 (69.56%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           M+FSS L  + L+F + ISP     SA    +++FL CLS   + +    KV++T  +SS
Sbjct: 1   MEFSSFLFTI-LLFSLNISP---LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           + S+LD SI+N  FS  E PKP+ IITP   S +Q  + C++ HG+ +RTRS GH +EGL
Sbjct: 61  FFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGL 120

Query: 121 SYVAY-RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPT 180
           SY+AY +PF V+DL NLRS+ +DV+N T WV++GAT GELYY IG+ +++LAFPAG+ PT
Sbjct: 121 SYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPT 180

Query: 181 VGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGSF 240
           VG+GG FSGGGYG +LRK+GLAADN+IDA +VDA G++LDR++MGED+FWAIRGGGG SF
Sbjct: 181 VGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSF 240

Query: 241 GIVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNAS 300
           G++++WK+KLV VP T+T+    +  +++A+++I +WQY  +K+ +++++   L   N +
Sbjct: 241 GVILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN 300

Query: 301 TQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTL---IIPIGV 360
                    A F  L++G V+ L+A +   FPELGL K+ C E SW+ES L     P G 
Sbjct: 301 A------VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG- 360

Query: 361 QPIESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGG 420
              ESL  L NR  T+L S K K D+V+EPIPEA I+ IW+RL+A +    ++I  P+GG
Sbjct: 361 ---ESLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGG 420

Query: 421 RMSQISESETPFPHRAGYLFKIGYVVGWKDQSLKAK---KRHISWIREVYEYMAPFVSKS 480
           +MS+++E ETPFPHR G L++I YV  W+++  K K    +++ W+  VYE+M P+VSKS
Sbjct: 421 KMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKS 480

Query: 481 PRAAYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQS 536
           PR AY N++D+D+G      KT Y+    WG+KYF +NF+RLV VKT+VDP DFF  EQS
Sbjct: 481 PRGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQS 527

BLAST of Chy12G210200 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 7.0e-149
Identity = 266/541 (49.17%), Postives = 366/541 (67.65%), Query Frame = 0

Query: 9   PLSLIFIIV-------ISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPIN--S 68
           PL L  I+V       I+P S  AS   Q    F++C+  ++   +P+ K  + P    S
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQDQ----FINCVQRNTHVYFPLEKTFFAPTKNVS 65

Query: 69  SYSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 128
            +S VL+ + +NL F K  +PKP  I +P H SH+QA++ICSK   + +R RSGGHD+EG
Sbjct: 66  MFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEG 125

Query: 129 LSYVAY--RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVC 188
           LSYV+   +PFI++DL  +R V +++++N+AWV+SGAT+GELYYRI EKS+   FPAG+C
Sbjct: 126 LSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLC 185

Query: 189 PTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGG 248
            ++GIGGH +GG YG M+RK+GL ADNV+DA +VDA+GK+LDR +MGED FWAIRGG GG
Sbjct: 186 SSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGG 245

Query: 249 SFGIVVAWKIKLVPVPPTVTICSTDRNLEED-AIKLIHRWQYVGNKLDENVYLGVILTGG 308
           SFGI++AWKIKLVPVP TVT+ +  + L++D   K+I +WQ V +KL E +++ V+    
Sbjct: 246 SFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF--- 305

Query: 309 NASTQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLIIPIGV 368
           N +   G    T  + +LFLG    LM  +  +FPELGL  +DC+E SW+ES   I    
Sbjct: 306 NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFP 365

Query: 369 QPIESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYGG 428
               +   L  ++P    S K KSD+VK PIPE+ ++GI+++L  +DI    +I+ PYGG
Sbjct: 366 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGG 425

Query: 429 RMSQISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPRA 488
            M++I ES+ PFPHR G LFK+ YV  W D S K   RHI+WIR++Y YM P+VS +PR 
Sbjct: 426 MMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPRE 485

Query: 489 AYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPT 538
           AY NYRDLD+G N K  KT  K+A +WG  YF +NF+RL+ +K KVDP +FFRHEQSIP 
Sbjct: 486 AYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 536

BLAST of Chy12G210200 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 521.5 bits (1342), Expect = 7.8e-148
Identity = 268/545 (49.17%), Postives = 360/545 (66.06%), Query Frame = 0

Query: 4   SSTLIPLSLIFIIVISPASLC---ASASPQKYQNFLHCLSEHSSKSYPISKVVYTPIN-- 63
           S  L  +S I + V+  +  C    S+S      F++C+  ++  S+P+ K ++TP    
Sbjct: 4   SKPLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNV 63

Query: 64  SSYSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFE 123
           S ++ VL+ + +NL F    +PKP  I  P H S +QA++ICSK  G+  R RSGGHDFE
Sbjct: 64  SLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFE 123

Query: 124 GLSYVA--YRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGV 183
            LSYV+   +PFI++DL  L+ + VD+E+N+AWV+ GATLGELYYRI EKS+   FPAG+
Sbjct: 124 ALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGL 183

Query: 184 CPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGG 243
           C +VGIGG+ +GGGYG ++RK+GLA DNV+D  +VDA+GK+LDR +MGED FWAIRGGGG
Sbjct: 184 CTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGG 243

Query: 244 GSFGIVVAWKIKLVPVPPTVTICSTDRNLEEDA-IKLIHRWQYVGNKLDENVYLGVILTG 303
            SFGIV+AWKIKLVPVP TVT+ +  + LE+DA +K I +WQ + +K+ E +++ V+L  
Sbjct: 244 ASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL-- 303

Query: 304 GNASTQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLI---I 363
             A+   G    T  +   FLG    L+  +   FPELGL ++DC E SW+E+ L     
Sbjct: 304 -RAAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGF 363

Query: 364 PIGVQPIESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFV 423
           P G  PIE L  L  ++P   D  K  SD+VKEPIP   ++GI++RL   +  T+ + + 
Sbjct: 364 PTG-SPIEILLQL--KSPLGKDYFKATSDFVKEPIPVIGLKGIFKRL--IEGNTTFLNWT 423

Query: 424 PYGGRMSQISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSK 483
           PYGG MS+I ES  PFPHR G LFKI Y   W +   K   R I+WI+E+Y YMAP+VS 
Sbjct: 424 PYGGMMSKIPESAIPFPHRNGTLFKILYYANWLEND-KTSSRKINWIKEIYNYMAPYVSS 483

Query: 484 SPRAAYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQ 538
           +PR AY NYRDLD G N    K ++  A IWG KYF  NFDRLV +KTKVDP +FFRHEQ
Sbjct: 484 NPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQ 539

BLAST of Chy12G210200 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.7e-147
Identity = 263/543 (48.43%), Postives = 365/543 (67.22%), Query Frame = 0

Query: 1   MKFSSTLIPLSLIFIIVISPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK++  L+   ++FI         +S+S    + F  CL+ +S   +PIS  ++   N S
Sbjct: 1   MKYALILVLFFVVFI-------WQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGS 60

Query: 61  YSSVLDFSIRNLLFSKPEIPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVL  +IRNL F+    PKP LII  +H SH+QAA+ C K H LQ++ RSGGHD++GL
Sbjct: 61  YSSVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGL 120

Query: 121 SYVAY--RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCP 180
           SYV Y  +PF V+D+ NLRSV VDV + TAWV++GA LGE+YY I EKS+TLA+PAG+CP
Sbjct: 121 SYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICP 180

Query: 181 TVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDFFWAIRGGGGGS 240
           TVG+GGH SGGGYG M+RK+GL  DN IDA +VD +GK+LDR+ MGED +WAI GGGGGS
Sbjct: 181 TVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGS 240

Query: 241 FGIVVAWKIKLVPVPPTVTICSTDRNLEEDAIKLIHRWQYVGNKLDENVYLGVILTGGNA 300
           +G+V+A+KI LV VP  VT+    R LE++A  +IHRWQ V  KL + +++  ++   N 
Sbjct: 241 YGVVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNG 300

Query: 301 STQAGITNPTARFFSLFLGRVDELMATLSTTFPELGLIKQDCVEASWVESTLI---IPIG 360
           +  +  T  T  F ++FLG    L++ L+  FPELGL++ DC E SW++S L    I +G
Sbjct: 301 TVSSQKTVRTT-FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVG 360

Query: 361 VQPIESLEPLLNRTPTNLDSTKIKSDYVKEPIPEATIEGIWQRLKAQDIETSQVIFVPYG 420
                S E LL +    ++  K KSDYV+EPI    +E IW+++   +I T  + F PYG
Sbjct: 361 -----SSETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYG 420

Query: 421 GRMSQISESETPFPHRAGYLFKIGYVVGWKDQSLKAKKRHISWIREVYEYMAPFVSKSPR 480
           G M +IS + TPFP+RAG L+KI Y   W+D++L    R++   R++Y++M PFVSK+PR
Sbjct: 421 GEMGRISSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPR 480

Query: 481 AAYTNYRDLDIGSNNKYGK-TSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSI 538
            ++ NYRD+D+G N+  GK +SY     +G KYF  NF+RLV +KT+VD  +FFR+EQSI
Sbjct: 481 QSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSI 526