Chy12G210140 (gene) Cucumber (hystrix) v1

Overview
NameChy12G210140
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionBerberine bridge enzyme-like
LocationchrH12: 4948525 .. 4950156 (-)
RNA-Seq ExpressionChy12G210140
SyntenyChy12G210140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCTCCAATTACCTCCTTTGCTCATATTTTTATTGTCTTGTCGTTTGCATGGGCAGCTTCTGCTCACAACCATGAATCTTTTCTCCACTGTCTTTCTCATCATTCTCCCAATACCTCTTCCATTTCAAAGATTATTTACACTCCAACCAATCCCTCATACTCTTCAGTTTTGAACTTCTCAATTCACAACCTTAGATTCACATCTCCTAAAACCCCTAAACCTCAAATCATCGTCACACCGTTCCATGTTTCTCAAATTCAAGCATCCATCATTTGTGCCAAAAACACAGGCTTTCAAATTCGAACTAGAAGCGGCGGTCATGACTACGAGGGACTTTCTTATGTCTCTGACGTCTCATTTGTCGTCGTTGATCTAATAAACCTTCGGTCGATTAGTGTCGATGAGGAAAACAATGTTGCATGGGTGCAATCTGGAGCCACCATTGGCGAACTCTACTACAGAATTGCTGAGAAAAGCAAAACCCTAGGGTTCCCAGCTGGGGTTTGTCCGACGGTTGGTGTCGGTGGACACTTCAGTGGCGGCGGCTATGGATTGATGCTACGTAAATTCGGCCTTGCTGCTGATAACGTGATCGACGCTTACTTCATTGACGTGAATGGTAAGCTCCACGACCGAAAATCAATGGGGGAGGATGTGTTTTGGGCCATTAGAGGAAGTGGAGGAGCGAGCTTTGGAATTGTCCTTGCATGGAAAATAAAGCTTCTCCCTGTTCCACCAATTGTGACTGTCTTCACAATCACAAGAACTTTGGAACAAAACGCCACAAACATCATTCACCGATGGCAATATGTCTCTAGCAAACAAGATGACAAACTTTTCATCAGAATCATATTAACAGGGATAAACACAACAAATTCTCAACATGGAAATAAAAGAACAATAGAAGCTGCTTTCAACTCCTTGTTCCTAGGAAAAATCGAAGAACTTGTTCCAATAATGCAAAAGACTTTCCCAGAACTAGGGTTAACAAAGGAAGATTGCATTGAAATGAGTTGGATTGAATCCGTTCTCTACTTTGCTGGCTTCTCAAGAGGACAACCATTAAATGTTCTTCTAGATAGAAGACCATTAACCCCCAAAAGATTTTTCAAAGCCAAATCAGACTATGTAAATGAGCCAATCCCAAAAGCTGGCCTTGAGGGCATATGGGAATTCTTTAATGAAGAAGAAGCAGAAGCGGCAGTTTTGATCTTGAGTCCGTATGGTGGAATAATGGACAAGATTTCAGCGTCAGAAATTCCATTCCCACACCGAGCTGGAAATCTTTACAAGATTCAGCATTTGGTGTATTGGGATGAGGAAGGTGAAGATATTGCTAAAAGACATATAAATTGGATCAGGAAACTTTATAGTTACATGGCTCCTTTAGTTTCAAAAAATCCAAGAGCGGCATATATCAATTACAGAGACCTGGATATTGGAACAAACGAGAAAAATGGTAGAAACACGAGCTACAACAAGGCAAGTGTGTGGGGAGTTAAATATTTCAAGGGTAATTTCAAGAGGTTGGTGAGTGTAAAAACAAAGGTTGATCCTTCTAATTTCTTCAAGAATGAGCAGAGCATTCCTTCTCTTAAGCCATGGTGGAATAAGAAGGGATACTAG

mRNA sequence

ATGAAGTCTCCAATTACCTCCTTTGCTCATATTTTTATTGTCTTGTCGTTTGCATGGGCAGCTTCTGCTCACAACCATGAATCTTTTCTCCACTGTCTTTCTCATCATTCTCCCAATACCTCTTCCATTTCAAAGATTATTTACACTCCAACCAATCCCTCATACTCTTCAGTTTTGAACTTCTCAATTCACAACCTTAGATTCACATCTCCTAAAACCCCTAAACCTCAAATCATCGTCACACCGTTCCATGTTTCTCAAATTCAAGCATCCATCATTTGTGCCAAAAACACAGGCTTTCAAATTCGAACTAGAAGCGGCGGTCATGACTACGAGGGACTTTCTTATGTCTCTGACGTCTCATTTGTCGTCGTTGATCTAATAAACCTTCGGTCGATTAGTGTCGATGAGGAAAACAATGTTGCATGGGTGCAATCTGGAGCCACCATTGGCGAACTCTACTACAGAATTGCTGAGAAAAGCAAAACCCTAGGGTTCCCAGCTGGGGTTTGTCCGACGGTTGGTGTCGGTGGACACTTCAGTGGCGGCGGCTATGGATTGATGCTACGTAAATTCGGCCTTGCTGCTGATAACGTGATCGACGCTTACTTCATTGACGTGAATGGTAAGCTCCACGACCGAAAATCAATGGGGGAGGATGTGTTTTGGGCCATTAGAGGAAGTGGAGGAGCGAGCTTTGGAATTGTCCTTGCATGGAAAATAAAGCTTCTCCCTGTTCCACCAATTGTGACTGTCTTCACAATCACAAGAACTTTGGAACAAAACGCCACAAACATCATTCACCGATGGCAATATGTCTCTAGCAAACAAGATGACAAACTTTTCATCAGAATCATATTAACAGGGATAAACACAACAAATTCTCAACATGGAAATAAAAGAACAATAGAAGCTGCTTTCAACTCCTTGTTCCTAGGAAAAATCGAAGAACTTGTTCCAATAATGCAAAAGACTTTCCCAGAACTAGGGTTAACAAAGGAAGATTGCATTGAAATGAGTTGGATTGAATCCGTTCTCTACTTTGCTGGCTTCTCAAGAGGACAACCATTAAATGTTCTTCTAGATAGAAGACCATTAACCCCCAAAAGATTTTTCAAAGCCAAATCAGACTATGTAAATGAGCCAATCCCAAAAGCTGGCCTTGAGGGCATATGGGAATTCTTTAATGAAGAAGAAGCAGAAGCGGCAGTTTTGATCTTGAGTCCGTATGGTGGAATAATGGACAAGATTTCAGCGTCAGAAATTCCATTCCCACACCGAGCTGGAAATCTTTACAAGATTCAGCATTTGGTGTATTGGGATGAGGAAGGTGAAGATATTGCTAAAAGACATATAAATTGGATCAGGAAACTTTATAGTTACATGGCTCCTTTAGTTTCAAAAAATCCAAGAGCGGCATATATCAATTACAGAGACCTGGATATTGGAACAAACGAGAAAAATGGTAGAAACACGAGCTACAACAAGGCAAGTGTGTGGGGAGTTAAATATTTCAAGGGTAATTTCAAGAGGTTGGTGAGTGTAAAAACAAAGGTTGATCCTTCTAATTTCTTCAAGAATGAGCAGAGCATTCCTTCTCTTAAGCCATGGTGGAATAAGAAGGGATACTAG

Coding sequence (CDS)

ATGAAGTCTCCAATTACCTCCTTTGCTCATATTTTTATTGTCTTGTCGTTTGCATGGGCAGCTTCTGCTCACAACCATGAATCTTTTCTCCACTGTCTTTCTCATCATTCTCCCAATACCTCTTCCATTTCAAAGATTATTTACACTCCAACCAATCCCTCATACTCTTCAGTTTTGAACTTCTCAATTCACAACCTTAGATTCACATCTCCTAAAACCCCTAAACCTCAAATCATCGTCACACCGTTCCATGTTTCTCAAATTCAAGCATCCATCATTTGTGCCAAAAACACAGGCTTTCAAATTCGAACTAGAAGCGGCGGTCATGACTACGAGGGACTTTCTTATGTCTCTGACGTCTCATTTGTCGTCGTTGATCTAATAAACCTTCGGTCGATTAGTGTCGATGAGGAAAACAATGTTGCATGGGTGCAATCTGGAGCCACCATTGGCGAACTCTACTACAGAATTGCTGAGAAAAGCAAAACCCTAGGGTTCCCAGCTGGGGTTTGTCCGACGGTTGGTGTCGGTGGACACTTCAGTGGCGGCGGCTATGGATTGATGCTACGTAAATTCGGCCTTGCTGCTGATAACGTGATCGACGCTTACTTCATTGACGTGAATGGTAAGCTCCACGACCGAAAATCAATGGGGGAGGATGTGTTTTGGGCCATTAGAGGAAGTGGAGGAGCGAGCTTTGGAATTGTCCTTGCATGGAAAATAAAGCTTCTCCCTGTTCCACCAATTGTGACTGTCTTCACAATCACAAGAACTTTGGAACAAAACGCCACAAACATCATTCACCGATGGCAATATGTCTCTAGCAAACAAGATGACAAACTTTTCATCAGAATCATATTAACAGGGATAAACACAACAAATTCTCAACATGGAAATAAAAGAACAATAGAAGCTGCTTTCAACTCCTTGTTCCTAGGAAAAATCGAAGAACTTGTTCCAATAATGCAAAAGACTTTCCCAGAACTAGGGTTAACAAAGGAAGATTGCATTGAAATGAGTTGGATTGAATCCGTTCTCTACTTTGCTGGCTTCTCAAGAGGACAACCATTAAATGTTCTTCTAGATAGAAGACCATTAACCCCCAAAAGATTTTTCAAAGCCAAATCAGACTATGTAAATGAGCCAATCCCAAAAGCTGGCCTTGAGGGCATATGGGAATTCTTTAATGAAGAAGAAGCAGAAGCGGCAGTTTTGATCTTGAGTCCGTATGGTGGAATAATGGACAAGATTTCAGCGTCAGAAATTCCATTCCCACACCGAGCTGGAAATCTTTACAAGATTCAGCATTTGGTGTATTGGGATGAGGAAGGTGAAGATATTGCTAAAAGACATATAAATTGGATCAGGAAACTTTATAGTTACATGGCTCCTTTAGTTTCAAAAAATCCAAGAGCGGCATATATCAATTACAGAGACCTGGATATTGGAACAAACGAGAAAAATGGTAGAAACACGAGCTACAACAAGGCAAGTGTGTGGGGAGTTAAATATTTCAAGGGTAATTTCAAGAGGTTGGTGAGTGTAAAAACAAAGGTTGATCCTTCTAATTTCTTCAAGAATGAGCAGAGCATTCCTTCTCTTAAGCCATGGTGGAATAAGAAGGGATACTAG

Protein sequence

MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNKKGY*
Homology
BLAST of Chy12G210140 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 271/527 (51.42%), Postives = 368/527 (69.83%), Query Frame = 0

Query: 11  IFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTP--TNPSYSSVLNFSIHNLRF 70
           + + LS+   + +   + F+ CL  +S  +  +    +T      S+   L  S  NLR+
Sbjct: 17  LLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLRY 76

Query: 71  TSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV--SFVVVD 130
             P  PKP+ I  P + + +QA+++CAK     +R RSGGHDYEGLSYVS++  +FV+VD
Sbjct: 77  LMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVD 136

Query: 131 LINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYG 190
           L  LR ISVD E+N AWV +GA+IGE+YYRI EKSK  GFPAG+C ++G+GGH  GG YG
Sbjct: 137 LSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYG 196

Query: 191 LMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPV 250
            M+RKFGL ADNV+DA  +D +GK+ +R +MGEDVFWAIRG GG SFG++LAWKIKL+PV
Sbjct: 197 SMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPV 256

Query: 251 PPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAA 310
           P IVTVFT+TRTLEQ+ T ++++WQ V+ K D+ LFIR+I+    T+ +    +RTI  +
Sbjct: 257 PEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQ--PTSKTPKSKERTISTS 316

Query: 311 FNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPL 370
           +   FLG    L+ +MQ++FP+LGLTK+DC+E SWI+SV+Y AGF    P   LLD + L
Sbjct: 317 YQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSL 376

Query: 371 TPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPH 430
             K +FKAKSDYV EPIP  GLEG+WE   EE  ++ + I +PYGG+M KI  +E PFPH
Sbjct: 377 F-KNYFKAKSDYVEEPIPVEGLEGLWEKLLEE--DSPLTIWNPYGGMMAKIPETETPFPH 436

Query: 431 RAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNE 490
           R+G L+KIQ L  W ++G+    +H+ W+R++YSYM   VSK+PR+AY+NYRDLD+G N 
Sbjct: 437 RSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNG 496

Query: 491 KNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPS 534
           K       + A  WG +YFKGNF+RLV +K K DP NFF++EQSIP+
Sbjct: 497 KG------SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of Chy12G210140 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 272/513 (53.02%), Postives = 361/513 (70.37%), Query Frame = 0

Query: 20  AASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFTSPKTPKPQII 79
           +A+  N  SFL CLS    +++ +SK+I+TP + S+SSVL  SI N RF++P  PKP +I
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 87

Query: 80  VTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLINLRSISVDEEN 139
           +TP   S +Q+++ CA+  G  IRTRSGGHDYEGLSYV+   FV++DL NLRSI+VD +N
Sbjct: 88  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 147

Query: 140 NVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNV 199
              WVQ+GATIGELYY I +K++TL FPAGVCPTVGVGGHFSGGGYG +LRK GLAAD+V
Sbjct: 148 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 207

Query: 200 IDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTITRTL 259
           IDA  +D  G++ +R+ MGED FWAIRG GG+SF +VL+WKI L+ VP  VTVF +T+  
Sbjct: 208 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 267

Query: 260 EQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELV 319
           EQ+A  IIHRWQ+V+ K  D LFIR++L            K  + A+F  L+LG ++ L+
Sbjct: 268 EQSALKIIHRWQFVADKVSDDLFIRVML---------QRYKNMVRASFPGLYLGSVKNLL 327

Query: 320 PIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKSDYV 379
            ++ K FPELGL ++DC EMSWIESV++FA     +P+NVL  R        FKAKSD+V
Sbjct: 328 KMVNKEFPELGLEEDDCTEMSWIESVIWFAELGE-EPINVLTKR--TRASLAFKAKSDFV 387

Query: 380 NEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKIQHLVY 439
            EP+PK  +  +W    E EAE A LI +P+GG M +I+  E PFPHR GN+Y+IQ+L Y
Sbjct: 388 QEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNY 447

Query: 440 WDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSYNKASV 499
           W     D+ ++++ W+ ++Y  M+  V+K+PR AYIN RDLD+G      + + Y +   
Sbjct: 448 W---RGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM-YVGVKRSKYEEGKS 507

Query: 500 WGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIP 533
           WGVKYFK NF+RLV VKT VDPS+FF +EQSIP
Sbjct: 508 WGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210140 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 270/513 (52.63%), Postives = 360/513 (70.18%), Query Frame = 0

Query: 27  ESFLHCLSHHSPNTSSISKIIYTPTN--PSYSSVLNFSIHNLRFTSPKTPKPQIIVTPFH 86
           + F++C+  ++     + K  + PT     +S VL  +  NLRF     PKP  I +P H
Sbjct: 33  DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIH 92

Query: 87  VSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVS--DVSFVVVDLINLRSISVDEENNVA 146
            S +QASIIC+K     +R RSGGHDYEGLSYVS  D  F+++DL  +R ++++ ++N A
Sbjct: 93  ESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSA 152

Query: 147 WVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNVIDA 206
           WVQSGAT+GELYYRIAEKSK  GFPAG+C ++G+GGH +GG YG M+RK+GL ADNV+DA
Sbjct: 153 WVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDA 212

Query: 207 YFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTITRTLEQN 266
             +D NGKL DR +MGED FWAIRG  G SFGI+LAWKIKL+PVP  VTVFT+T+TL+Q+
Sbjct: 213 KIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQD 272

Query: 267 ATN-IIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELVPI 326
             N II +WQ V+ K  ++LFIR++     T     G  +T+  ++N+LFLG    L+ +
Sbjct: 273 VGNKIISKWQRVADKLVEELFIRVLFNVAGT-----GGNKTVTTSYNALFLGGKGTLMNV 332

Query: 327 MQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKSDYVNE 386
           M+K+FPELGLT +DCIEMSW+ES+ Y +GF    P NVLL  +   PK  FKAKSD+V  
Sbjct: 333 MKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKT 392

Query: 387 PIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKIQHLVYWD 446
           PIP++GL+GI++   +E  +  ++I +PYGG+M KI  S+IPFPHR G L+K+Q++  W 
Sbjct: 393 PIPESGLQGIFKKLLKE--DIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSW- 452

Query: 447 EEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSYNKASVWG 506
            + +    RHINWIR LYSYM P VS NPR AY+NYRDLD+G N K+ + T   +A VWG
Sbjct: 453 LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVK-TCIKQAQVWG 512

Query: 507 VKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 535
             YFK NF RL+ +K KVDP NFF++EQSIP +
Sbjct: 513 ANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Chy12G210140 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 2.9e-155
Identity = 269/517 (52.03%), Postives = 365/517 (70.60%), Query Frame = 0

Query: 21  ASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPS--YSSVLNFSIHNLRFTSPKTPKPQI 80
           +S+   + F+ CL  +S  +  I+   ++P   +  +   L  +  NLR+ +P  PKP  
Sbjct: 25  SSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVF 84

Query: 81  IVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVS--DVSFVVVDLINLRSISVD 140
           I  P + + +QA+++CAK     +R RSGGHDYEGLS+V+  +  FV+VDL  LR + VD
Sbjct: 85  IFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVD 144

Query: 141 EENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAA 200
            ++N AW  +GATIGE+YYRI EKS+T GFPAG+C ++G+GGH  GG YG M+RKFGL A
Sbjct: 145 LDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGA 204

Query: 201 DNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTIT 260
           DNV+DA  +D NG++ DR +MGEDVFWAIRG GG SFG++LAWKIKL+PVP  VTVFT+T
Sbjct: 205 DNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVT 264

Query: 261 RTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIE 320
           +TLEQ+ T ++++W+ ++ K DD LFIR+I++  + T ++ GN RTI  ++ + FLG   
Sbjct: 265 KTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKT-TKPGN-RTISMSYQAQFLGDSN 324

Query: 321 ELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKS 380
            L+ +MQK+FPELGLTK+DC EMSWI+SV+Y AGF        LL  + L  K  FKAKS
Sbjct: 325 RLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSLF-KNHFKAKS 384

Query: 381 DYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKIQH 440
           D+V EPIP  GLEG+WE F EE  ++ + I +PYGG+M +IS SEIPFPHR G L+KIQ 
Sbjct: 385 DFVKEPIPVEGLEGLWERFLEE--DSPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQW 444

Query: 441 LVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSYNK 500
           L  W ++G+   +RH+ WIR++YSYM   VSKNPR AY+NYRDLD+GTNE          
Sbjct: 445 LSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE------GETD 504

Query: 501 ASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPS 534
           A  WG KY+KGNF+RLV +K + DP NFF++EQS+P+
Sbjct: 505 AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Chy12G210140 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.5e-154
Identity = 270/530 (50.94%), Postives = 369/530 (69.62%), Query Frame = 0

Query: 11  IFIVLSFAWAAS-AHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFT 70
           IF   SF    S A+  E+FL C S + PN ++  K++YT  NP Y SVLN +IHNLRFT
Sbjct: 15  IFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFT 74

Query: 71  SPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLIN 130
           S  TPKP +IVTP HVS IQ +I+C+K  G QIRTRSGGHD EG+SY+S V FV+VDL N
Sbjct: 75  SDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRN 134

Query: 131 LRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLML 190
           +RSI +D  +  AWV++GAT+GE+YY + EK++ L   AG CPTV  GGHF GGGYG ++
Sbjct: 135 MRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLM 194

Query: 191 RKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPI 250
           R +GLAADN+IDA+ ++V+GK+ DRKSMGED+FWA+RG G  SFGI++AWKI+L+ VP  
Sbjct: 195 RNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK- 254

Query: 251 VTVFTITRTLE-QNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFN 310
            T+F++ + +E      ++++WQ ++ K D  L +       N T++Q  NK  I   F+
Sbjct: 255 STMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFS 314

Query: 311 SLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLN----VLLDRR 370
           S+FLG ++ LV +M K+FPELG+ K DC ++SWI+++++++G       N    +LLD R
Sbjct: 315 SVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLD-R 374

Query: 371 PLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPF 430
                  FK K DYV +PIP++    I E   EE+  A +  L PYGGIMD+IS S IPF
Sbjct: 375 SAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPF 434

Query: 431 PHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGT 490
           PHRAG LY++ ++  W+++ ++  ++H+NWIR +Y++M P VSKNPR AY+NYRDLDIG 
Sbjct: 435 PHRAGILYELWYICSWEKQEDN--EKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGI 494

Query: 491 NEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 535
           N+    N +Y +A +WG KYF  NF RLV VKT VDP+NFF+NEQSIP L
Sbjct: 495 NDPKNPN-NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Chy12G210140 vs. ExPASy TrEMBL
Match: A0A5A7U0J8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5482G00070 PE=3 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.0e-310
Identity = 526/543 (96.87%), Postives = 532/543 (97.97%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKS ITSF HIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRFTSPKTPKP IIVTPF VSQIQASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SFVVVDLINLRSISVD ENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRG GGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLLPVP IVTVFTITRTLEQNAT++IHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RTIEAAFNSLFLGKIEELVPIM+KTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWE F+EEEAEAAVLILSPYGGIMDKIS S
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAP VSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIGTNEKNGRNTSYN+ASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

Query: 541 KGY 544
           +GY
Sbjct: 541 RGY 543

BLAST of Chy12G210140 vs. ExPASy TrEMBL
Match: A0A1S3BE31 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488607 PE=3 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.0e-310
Identity = 526/543 (96.87%), Postives = 532/543 (97.97%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKS ITSF HIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRFTSPKTPKP IIVTPF VSQIQASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SFVVVDLINLRSISVD ENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRG GGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLLPVP IVTVFTITRTLEQNAT++IHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RTIEAAFNSLFLGKIEELVPIM+KTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWE F+EEEAEAAVLILSPYGGIMDKIS S
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAP VSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIGTNEKNGRNTSYN+ASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

Query: 541 KGY 544
           +GY
Sbjct: 541 RGY 543

BLAST of Chy12G210140 vs. ExPASy TrEMBL
Match: A0A6J1GTC1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.6e-276
Identity = 459/543 (84.53%), Postives = 500/543 (92.08%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MK PIT F + F+VLSFAWAASAHNHE FLHCLS HSPN+SSISKIIYTP + SYSSVLN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRF +P+TPKPQ+IVTP  V++I+ASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            F+VVDLINLRSI+VD ENNVAWVQSGATIGELYYRIA+KSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNVIDAYFIDVNG LHDRKSMGEDVFWA+RG GGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLL VPPIVTVFT+TR LEQNATN+IHRWQY++SKQDD+LFIRII+ G+N+TN QHG++
Sbjct: 241 IKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQ 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RT+EAAFNS+FLGK EEL+PIMQ TFPELGLTKEDCIEMSWIES+LYFAGF  GQPLNVL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDR PL PKRFFKAKSDYVNEPIPKA LEG WE FNEEEAEAA +ILSPYGGIMD+IS S
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYW+EEGEDIA+RHINWIRKLYS++ P VSKNPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIG N KNG+ TSY +ASVWGVKYFKGNFKRLVS+KTKVDPSNFF NEQSIPS+ P W K
Sbjct: 481 DIGMNNKNGK-TSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWKK 540

Query: 541 KGY 544
           +G+
Sbjct: 541 RGH 542

BLAST of Chy12G210140 vs. ExPASy TrEMBL
Match: A0A6J1K043 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.4e-276
Identity = 458/543 (84.35%), Postives = 498/543 (91.71%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKSPIT F + F+VLSFAWAASAHNHE FLHCLS HSPN+SSISK IYTP + SYSSVLN
Sbjct: 1   MKSPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKTIYTPNDSSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRF +P+TPKPQ+IVTP  VS+I+ASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPRTPKPQVIVTPLQVSEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            F++VDLINLRSI+VD +NNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIIVDLINLRSINVDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNVIDAYFIDVNG LHDRKSMGEDVFWA+RG GGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLL VPPIVTVFT+ RTLEQNATN+IHRWQY++SKQDD+LFIRII+ G+N+TN Q G++
Sbjct: 241 IKLLQVPPIVTVFTVPRTLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQQGSQ 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RT+EAAFNS+FLGK EEL+PIMQ TFPELGLTKEDCIEMSWIES+LYFAGF  GQPLNVL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDR PL PKRFFKAKSDYVNEPIPKA LEG WE F EEEAEAA +ILSPYGGIMD+IS S
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFKEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYW+EEGEDIA+RHINWIRKLYSY+AP VS NPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSYVAPYVSNNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIG N KNG+ TSY +ASVWGVKYFKGNFKRLVS+KTKVDPSNFF NEQSIPS+ PWW K
Sbjct: 481 DIGMNNKNGK-TSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPWWKK 540

Query: 541 KGY 544
           + +
Sbjct: 541 RAH 542

BLAST of Chy12G210140 vs. ExPASy TrEMBL
Match: A0A6J1DG91 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020167 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 8.8e-272
Identity = 455/542 (83.95%), Postives = 497/542 (91.70%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKSPIT  A+ F+VL F  AASAHNHE FLHCLS HSPN SSIS IIYTPTNPSYSSVLN
Sbjct: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKPQ+IVTP  VSQ+QASI+CAKN GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RG GGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLLPVPP+VTVFT+TR LEQNAT+++HRWQYV+ KQDDKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFP LGL KEDCI+MSWIES+LYFAGF RG+ L VL
Sbjct: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDRRPL PKRFFKAKSDYVNEPIPK GLEGIW+ FNEE+AEAA +ILSPYGG M++IS S
Sbjct: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+AP VSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIGTN KNG NTSY +AS+WGVKYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEK 539

Query: 541 KG 543
           +G
Sbjct: 541 RG 539

BLAST of Chy12G210140 vs. NCBI nr
Match: XP_004135722.1 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653302.1 hypothetical protein Csa_023170 [Cucumis sativus])

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 531/543 (97.79%), Postives = 538/543 (99.08%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKS ITSFAHIFIVL+FAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFAHIFIVLTFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRFTSPKTPKPQ+IVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SFVVVDLINLRSISVD ENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGAS+GIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLLPVPPIVTVFTI RTLEQNAT+IIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK
Sbjct: 241 IKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKIS S
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAP VSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIGTNEKNGRNTSYN+ASVWG+KYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGIKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

Query: 541 KGY 543
           +GY
Sbjct: 541 RGY 543

BLAST of Chy12G210140 vs. NCBI nr
Match: XP_008445653.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047121.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK24891.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1063 bits (2749), Expect = 0.0
Identity = 526/543 (96.87%), Postives = 532/543 (97.97%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKS ITSF HIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRFTSPKTPKP IIVTPF VSQIQASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SFVVVDLINLRSISVD ENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRG GGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLLPVP IVTVFTITRTLEQNAT++IHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RTIEAAFNSLFLGKIEELVPIM+KTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWE F+EEEAEAAVLILSPYGGIMDKIS S
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAP VSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIGTNEKNGRNTSYN+ASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

Query: 541 KGY 543
           +GY
Sbjct: 541 RGY 543

BLAST of Chy12G210140 vs. NCBI nr
Match: XP_038895082.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 1008 bits (2607), Expect = 0.0
Identity = 495/543 (91.16%), Postives = 517/543 (95.21%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKS ITSFA IFIVLSFAWAASA NHE+FLHCLS H PNTSSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSQITSFACIFIVLSFAWAASADNHEAFLHCLSLHYPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRFTSPKTPKPQ+I+TPF V Q+QASIICAKNTGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPQVIITPFQVFQVQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            FVVVDLINLRSISVD ENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFVVVDLINLRSISVDVENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNVIDAYFIDV+GKLHDRKSMGEDVFWAIRG GGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVDGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLLPVPP VTVFT+TR LEQNATN+I++WQY+SSKQD+ LFIRIIL GINTTNSQ GNK
Sbjct: 241 IKLLPVPPTVTVFTVTRNLEQNATNLINQWQYISSKQDENLFIRIILRGINTTNSQDGNK 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RTIEAAFNSLFLGKIE+L+ IMQ+TFPELGLTKEDCIEMSWIES+LYFAGFSRGQPLNVL
Sbjct: 301 RTIEAAFNSLFLGKIEQLLQIMQETFPELGLTKEDCIEMSWIESILYFAGFSRGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDRRPL PKRFFKAKSDYVNEPIPKAGLEGIWE FNEEEAEAA +ILSPYGGIMDKIS S
Sbjct: 361 LDRRPLFPKRFFKAKSDYVNEPIPKAGLEGIWELFNEEEAEAAEMILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           EIPFPHR GNLYKIQHLVYW+EEGEDIA+RHINWIRKLYSYMAP VSKNPR AYINYRDL
Sbjct: 421 EIPFPHREGNLYKIQHLVYWEEEGEDIAQRHINWIRKLYSYMAPFVSKNPRLAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIG NEKNGRNTSY++ASVWGVKYFKGNFKRLVSVKTK+DPSNFFKNEQSIPSL PWW K
Sbjct: 481 DIGMNEKNGRNTSYSEASVWGVKYFKGNFKRLVSVKTKIDPSNFFKNEQSIPSLLPWWKK 540

Query: 541 KGY 543
           +GY
Sbjct: 541 RGY 543

BLAST of Chy12G210140 vs. NCBI nr
Match: KAG6573420.1 (Berberine bridge enzyme-like 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 459/543 (84.53%), Postives = 500/543 (92.08%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MKSPIT F + F+VLSFAWAASAHNHE FLHCLS HSPN+SSISK IYTP + SYSSVLN
Sbjct: 1   MKSPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKTIYTPNDSSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRF +P+TPKPQIIVTP  V++I+ASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPRTPKPQIIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            F+VVDLINLRSI++D +NNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIVVDLINLRSINIDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNVIDAYFIDVNG LHDRKSMGEDVFWA+RG GGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLL VPPIVTVFT+TR LEQNATN+IHRWQY++SKQDD+LFIRII+ GIN+TN QHG++
Sbjct: 241 IKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGINSTNPQHGSQ 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RT+EAAFNS+FLGK EEL+PIMQ TFPELGLTKEDCIEMSWIES+LYFAGF  GQPLNVL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDR PL PKRFFKAKSDYVNEPIPKA LEG WE FNEEEAEAA +ILSPYGGIMD+IS S
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYW+EEGEDIA+RHINWIRKLYS++ P VSKNPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           D+G N KNG+ TSY +ASVWGVKYFKGNFKRLVS+KTKVDPSNFF NEQSIPS+ P W K
Sbjct: 481 DVGMNNKNGK-TSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWKK 540

Query: 541 KGY 543
           +G+
Sbjct: 541 RGH 542

BLAST of Chy12G210140 vs. NCBI nr
Match: XP_022954564.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 459/543 (84.53%), Postives = 500/543 (92.08%), Query Frame = 0

Query: 1   MKSPITSFAHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60
           MK PIT F + F+VLSFAWAASAHNHE FLHCLS HSPN+SSISKIIYTP + SYSSVLN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSIHNLRFTSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120
           FSIHNLRF +P+TPKPQ+IVTP  V++I+ASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFVVVDLINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            F+VVDLINLRSI+VD ENNVAWVQSGATIGELYYRIA+KSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNVIDAYFIDVNG LHDRKSMGEDVFWA+RG GGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300
           IKLL VPPIVTVFT+TR LEQNATN+IHRWQY++SKQDD+LFIRII+ G+N+TN QHG++
Sbjct: 241 IKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQ 300

Query: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360
           RT+EAAFNS+FLGK EEL+PIMQ TFPELGLTKEDCIEMSWIES+LYFAGF  GQPLNVL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISAS 420
           LDR PL PKRFFKAKSDYVNEPIPKA LEG WE FNEEEAEAA +ILSPYGGIMD+IS S
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYW+EEGEDIA+RHINWIRKLYS++ P VSKNPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDL 480

Query: 481 DIGTNEKNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540
           DIG N KNG+ TSY +ASVWGVKYFKGNFKRLVS+KTKVDPSNFF NEQSIPS+ P W K
Sbjct: 481 DIGMNNKNGK-TSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWKK 540

Query: 541 KGY 543
           +G+
Sbjct: 541 RGH 542

BLAST of Chy12G210140 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 551.2 bits (1419), Expect = 9.3e-157
Identity = 271/527 (51.42%), Postives = 368/527 (69.83%), Query Frame = 0

Query: 11  IFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTP--TNPSYSSVLNFSIHNLRF 70
           + + LS+   + +   + F+ CL  +S  +  +    +T      S+   L  S  NLR+
Sbjct: 17  LLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLRY 76

Query: 71  TSPKTPKPQIIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV--SFVVVD 130
             P  PKP+ I  P + + +QA+++CAK     +R RSGGHDYEGLSYVS++  +FV+VD
Sbjct: 77  LMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVD 136

Query: 131 LINLRSISVDEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYG 190
           L  LR ISVD E+N AWV +GA+IGE+YYRI EKSK  GFPAG+C ++G+GGH  GG YG
Sbjct: 137 LSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYG 196

Query: 191 LMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPV 250
            M+RKFGL ADNV+DA  +D +GK+ +R +MGEDVFWAIRG GG SFG++LAWKIKL+PV
Sbjct: 197 SMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPV 256

Query: 251 PPIVTVFTITRTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAA 310
           P IVTVFT+TRTLEQ+ T ++++WQ V+ K D+ LFIR+I+    T+ +    +RTI  +
Sbjct: 257 PEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQ--PTSKTPKSKERTISTS 316

Query: 311 FNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPL 370
           +   FLG    L+ +MQ++FP+LGLTK+DC+E SWI+SV+Y AGF    P   LLD + L
Sbjct: 317 YQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSL 376

Query: 371 TPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPH 430
             K +FKAKSDYV EPIP  GLEG+WE   EE  ++ + I +PYGG+M KI  +E PFPH
Sbjct: 377 F-KNYFKAKSDYVEEPIPVEGLEGLWEKLLEE--DSPLTIWNPYGGMMAKIPETETPFPH 436

Query: 431 RAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNE 490
           R+G L+KIQ L  W ++G+    +H+ W+R++YSYM   VSK+PR+AY+NYRDLD+G N 
Sbjct: 437 RSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNG 496

Query: 491 KNGRNTSYNKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPS 534
           K       + A  WG +YFKGNF+RLV +K K DP NFF++EQSIP+
Sbjct: 497 KG------SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of Chy12G210140 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 551.2 bits (1419), Expect = 9.3e-157
Identity = 272/513 (53.02%), Postives = 361/513 (70.37%), Query Frame = 0

Query: 20  AASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFTSPKTPKPQII 79
           +A+  N  SFL CLS    +++ +SK+I+TP + S+SSVL  SI N RF++P  PKP +I
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 87

Query: 80  VTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLINLRSISVDEEN 139
           +TP   S +Q+++ CA+  G  IRTRSGGHDYEGLSYV+   FV++DL NLRSI+VD +N
Sbjct: 88  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 147

Query: 140 NVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNV 199
              WVQ+GATIGELYY I +K++TL FPAGVCPTVGVGGHFSGGGYG +LRK GLAAD+V
Sbjct: 148 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 207

Query: 200 IDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTITRTL 259
           IDA  +D  G++ +R+ MGED FWAIRG GG+SF +VL+WKI L+ VP  VTVF +T+  
Sbjct: 208 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 267

Query: 260 EQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELV 319
           EQ+A  IIHRWQ+V+ K  D LFIR++L            K  + A+F  L+LG ++ L+
Sbjct: 268 EQSALKIIHRWQFVADKVSDDLFIRVML---------QRYKNMVRASFPGLYLGSVKNLL 327

Query: 320 PIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKSDYV 379
            ++ K FPELGL ++DC EMSWIESV++FA     +P+NVL  R        FKAKSD+V
Sbjct: 328 KMVNKEFPELGLEEDDCTEMSWIESVIWFAELGE-EPINVLTKR--TRASLAFKAKSDFV 387

Query: 380 NEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKIQHLVY 439
            EP+PK  +  +W    E EAE A LI +P+GG M +I+  E PFPHR GN+Y+IQ+L Y
Sbjct: 388 QEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNY 447

Query: 440 WDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSYNKASV 499
           W     D+ ++++ W+ ++Y  M+  V+K+PR AYIN RDLD+G      + + Y +   
Sbjct: 448 W---RGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM-YVGVKRSKYEEGKS 507

Query: 500 WGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIP 533
           WGVKYFK NF+RLV VKT VDPS+FF +EQSIP
Sbjct: 508 WGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210140 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 551.2 bits (1419), Expect = 9.3e-157
Identity = 270/513 (52.63%), Postives = 360/513 (70.18%), Query Frame = 0

Query: 27  ESFLHCLSHHSPNTSSISKIIYTPTN--PSYSSVLNFSIHNLRFTSPKTPKPQIIVTPFH 86
           + F++C+  ++     + K  + PT     +S VL  +  NLRF     PKP  I +P H
Sbjct: 33  DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIH 92

Query: 87  VSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVS--DVSFVVVDLINLRSISVDEENNVA 146
            S +QASIIC+K     +R RSGGHDYEGLSYVS  D  F+++DL  +R ++++ ++N A
Sbjct: 93  ESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSA 152

Query: 147 WVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNVIDA 206
           WVQSGAT+GELYYRIAEKSK  GFPAG+C ++G+GGH +GG YG M+RK+GL ADNV+DA
Sbjct: 153 WVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDA 212

Query: 207 YFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTITRTLEQN 266
             +D NGKL DR +MGED FWAIRG  G SFGI+LAWKIKL+PVP  VTVFT+T+TL+Q+
Sbjct: 213 KIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQD 272

Query: 267 ATN-IIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELVPI 326
             N II +WQ V+ K  ++LFIR++     T     G  +T+  ++N+LFLG    L+ +
Sbjct: 273 VGNKIISKWQRVADKLVEELFIRVLFNVAGT-----GGNKTVTTSYNALFLGGKGTLMNV 332

Query: 327 MQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKSDYVNE 386
           M+K+FPELGLT +DCIEMSW+ES+ Y +GF    P NVLL  +   PK  FKAKSD+V  
Sbjct: 333 MKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKT 392

Query: 387 PIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKIQHLVYWD 446
           PIP++GL+GI++   +E  +  ++I +PYGG+M KI  S+IPFPHR G L+K+Q++  W 
Sbjct: 393 PIPESGLQGIFKKLLKE--DIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSW- 452

Query: 447 EEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSYNKASVWG 506
            + +    RHINWIR LYSYM P VS NPR AY+NYRDLD+G N K+ + T   +A VWG
Sbjct: 453 LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVK-TCIKQAQVWG 512

Query: 507 VKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 535
             YFK NF RL+ +K KVDP NFF++EQSIP +
Sbjct: 513 ANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Chy12G210140 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 550.1 bits (1416), Expect = 2.1e-156
Identity = 269/517 (52.03%), Postives = 365/517 (70.60%), Query Frame = 0

Query: 21  ASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPS--YSSVLNFSIHNLRFTSPKTPKPQI 80
           +S+   + F+ CL  +S  +  I+   ++P   +  +   L  +  NLR+ +P  PKP  
Sbjct: 25  SSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVF 84

Query: 81  IVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVS--DVSFVVVDLINLRSISVD 140
           I  P + + +QA+++CAK     +R RSGGHDYEGLS+V+  +  FV+VDL  LR + VD
Sbjct: 85  IFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVD 144

Query: 141 EENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAA 200
            ++N AW  +GATIGE+YYRI EKS+T GFPAG+C ++G+GGH  GG YG M+RKFGL A
Sbjct: 145 LDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGA 204

Query: 201 DNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTIT 260
           DNV+DA  +D NG++ DR +MGEDVFWAIRG GG SFG++LAWKIKL+PVP  VTVFT+T
Sbjct: 205 DNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVT 264

Query: 261 RTLEQNATNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIE 320
           +TLEQ+ T ++++W+ ++ K DD LFIR+I++  + T ++ GN RTI  ++ + FLG   
Sbjct: 265 KTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKT-TKPGN-RTISMSYQAQFLGDSN 324

Query: 321 ELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKS 380
            L+ +MQK+FPELGLTK+DC EMSWI+SV+Y AGF        LL  + L  K  FKAKS
Sbjct: 325 RLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSLF-KNHFKAKS 384

Query: 381 DYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKIQH 440
           D+V EPIP  GLEG+WE F EE  ++ + I +PYGG+M +IS SEIPFPHR G L+KIQ 
Sbjct: 385 DFVKEPIPVEGLEGLWERFLEE--DSPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQW 444

Query: 441 LVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSYNK 500
           L  W ++G+   +RH+ WIR++YSYM   VSKNPR AY+NYRDLD+GTNE          
Sbjct: 445 LSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE------GETD 504

Query: 501 ASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPS 534
           A  WG KY+KGNF+RLV +K + DP NFF++EQS+P+
Sbjct: 505 AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Chy12G210140 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 543.1 bits (1398), Expect = 2.5e-154
Identity = 272/520 (52.31%), Postives = 358/520 (68.85%), Query Frame = 0

Query: 20  AASAHNHESFLHCLSHHSPNTSSISKIIYTPTN--PSYSSVLNFSIHNLRFTSPKTPKPQ 79
           ++SA   + F++C+  ++  +  + K ++TP      ++ VL  +  NL+F +   PKP 
Sbjct: 29  SSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKPG 88

Query: 80  IIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVS--FVVVDLINLRSISV 139
            I  P H SQ+QASIIC+K  G   R RSGGHD+E LSYVS +   F+++DL  L+ I+V
Sbjct: 89  FIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQINV 148

Query: 140 DEENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLA 199
           D E+N AWVQ GAT+GELYYRIAEKSK  GFPAG+C +VG+GG+ +GGGYG ++RK+GLA
Sbjct: 149 DIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGLA 208

Query: 200 ADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASFGIVLAWKIKLLPVPPIVTVFTI 259
            DNV+D   +D NGKL DR +MGED+FWAIRG GGASFGIVLAWKIKL+PVP  VTVFT+
Sbjct: 209 GDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFTV 268

Query: 260 TRTLEQNA-TNIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGK 319
           T+TLEQ+A    I +WQ +SSK  +++ IR++L          GNK T+   +   FLG+
Sbjct: 269 TKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAA----GNDGNK-TVTMTYLGQFLGE 328

Query: 320 IEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKA 379
              L+ +M+K FPELGLT++DC EMSWIE+ L+  GF  G P+ +LL  +    K +FKA
Sbjct: 329 KGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGKDYFKA 388

Query: 380 KSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISASEIPFPHRAGNLYKI 439
            SD+V EPIP  GL+GI  F    E     L  +PYGG+M KI  S IPFPHR G L+KI
Sbjct: 389 TSDFVKEPIPVIGLKGI--FKRLIEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKI 448

Query: 440 QHLVYWDEEGEDIAKRHINWIRKLYSYMAPLVSKNPRAAYINYRDLDIGTNEKNGRNTSY 499
            +   W  E +  + R INWI+++Y+YMAP VS NPR AY+NYRDLD G N KN    ++
Sbjct: 449 LYYANW-LENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQN-KNNAKVNF 508

Query: 500 NKASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 535
            +A +WG KYFKGNF RLV +KTKVDP NFF++EQSIP +
Sbjct: 509 IEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93ZA31.3e-15551.42Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=... [more]
Q9SVG51.3e-15553.02Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
Q9FKU81.3e-15552.63Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... [more]
O647432.9e-15552.03Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
A6P6V92.5e-15450.94Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7U0J82.0e-31096.87Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BE312.0e-31096.87tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348860... [more]
A0A6J1GTC12.6e-27684.53berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE... [more]
A0A6J1K0433.4e-27684.35berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3... [more]
A0A6J1DG918.8e-27283.95berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020167 P... [more]
Match NameE-valueIdentityDescription
XP_004135722.10.097.79berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653302.1 hypothetical pro... [more]
XP_008445653.10.096.87PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047121.... [more]
XP_038895082.10.091.16berberine bridge enzyme-like 18 [Benincasa hispida][more]
KAG6573420.10.084.53Berberine bridge enzyme-like 18, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022954564.10.084.53berberine bridge enzyme-like 18 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G30760.19.3e-15751.42FAD-binding Berberine family protein [more]
AT4G20820.19.3e-15753.02FAD-binding Berberine family protein [more]
AT5G44400.19.3e-15752.63FAD-binding Berberine family protein [more]
AT2G34790.12.1e-15652.03FAD-binding Berberine family protein [more]
AT5G44380.12.5e-15452.31FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 134..532
e-value: 9.3E-171
score: 570.4
NoneNo IPR availableGENE3D3.40.462.20coord: 249..469
e-value: 9.3E-171
score: 570.4
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 23..534
NoneNo IPR availablePANTHERPTHR32448:SF113BERBERINE BRIDGE ENZYME-LIKE 18coord: 23..534
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 23..130
e-value: 1.7E-44
score: 152.1
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 473..532
e-value: 3.6E-22
score: 78.2
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 76..212
e-value: 6.1E-26
score: 90.8
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 72..246
score: 17.229403
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 19..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G210140.1Chy12G210140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding