Chy12G210110 (gene) Cucumber (hystrix) v1

BLAST of Chy12G210110 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 3.3e-196
Identity = 340/526 (64.64%), Postives = 410/526 (77.95%), Query Frame = 0

Query: 8   ILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 67
           IL+L F   +  SSSSS+  E F QCL  +S P +PI+PAIF   N SYSSVL+  IRNL
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNL 65

Query: 68  RFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFI 127
           RFNT++TPKPFLI+ A HESHV+AAI C + HNLQMKIRSGGHDY+G+SYV+    PFF+
Sbjct: 66  RFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFV 125

Query: 128 LDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDMFNLR + VD  +KTAW QTGA LGEVYY + EK+K+  +PAG+CPTVGVGGH  GGG
Sbjct: 126 LDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGG 185

Query: 188 YGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNMMRKYGL+VD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AYKI +V
Sbjct: 186 YGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLV 245

Query: 248 PVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRAT 307
            VPE VTVFR+ RTLEQN  T+I+  WQQVA  + ++LFIR   DVVNGT   +KT+R T
Sbjct: 246 EVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 305

Query: 308 FIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALLSRTPQ 367
           FIA+FLGD+  LL ++N  FP+LGL +SDC E SW+QSVLFWTN  +G+S E LL +  Q
Sbjct: 306 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQ 365

Query: 368 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 427
            + +LKRKSDYV+ PI + GL  IWKKMIELE P + FNPYGG M  I S  TPFP+RAG
Sbjct: 366 PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAG 425

Query: 428 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN-HNG 487
           NLWKIQY  NW +E   +   +++LTRKLY+FMTPFVSKNPR +F NYRD+D+G+N HNG
Sbjct: 426 NLWKIQYGANWRDETLTD--RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 488 K-NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           K +SY EG+ YG KYF GNF+RLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Chy12G210110 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 1.2e-164
Identity = 297/523 (56.79%), Postives = 375/523 (71.70%), Query Frame = 0

Query: 15  FLVSFSSSSSSAP-------ENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRN 74
           FLVS    SS+AP       E+F QC   +   P   I   +F+  N S+SSVL  YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 75  LRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFI 134
            RFNTS+TPKP +I+T   + HV AA+ C++  N  +KIRSGGHDYEG+SY+SD  PFFI
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISD-KPFFI 135

Query: 135 LDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 194
           LDM NLR+++VD   ++AW   GATLGEVYYR+ EK+K +GFPAGVCPTVGVGGH  GGG
Sbjct: 136 LDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGG 195

Query: 195 YGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 254
           YGNM+RK+GLSVD +IDAKI+DVNG++LDRK+MGEDLFWAI GGGG+SFGVV+ YK+++V
Sbjct: 196 YGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLV 255

Query: 255 PVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRAT 314
           PVPETVTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RAT
Sbjct: 256 PVPETVTVFRVEKYMD-SGAVDMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRAT 315

Query: 315 FIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRT 374
            +ALFLG +E ++ ++   FP+L LKK +C EM+W QS L+W N    T ++    L R 
Sbjct: 316 VVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRN 375

Query: 375 PQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHR 434
                  KRKSDYV + IP++G+  ++KKM EL K  L FNPYGG+MAE+  NATPFPHR
Sbjct: 376 LDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHR 435

Query: 435 AGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHN 494
           +  L+KIQY+  W E   +  K F++    LY FMT FVSKNPR A+LNYRD+DIGVN +
Sbjct: 436 S-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDH 495

Query: 495 GKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           G NSY EG VYG KYF  NFDRLVK+KT  DP NFFRNEQSIP
Sbjct: 496 GTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Chy12G210110 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 572.0 bits (1473), Expect = 7.0e-162
Identity = 290/521 (55.66%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 10  LLSFSFLVSFSSSSSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLR 69
           LL  S  +S  S S+S   +F +C   +   P   I   +F+  N ++SSVL  YIRN R
Sbjct: 19  LLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNAR 78

Query: 70  FNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFILD 129
           FNTS+T KP +I+T   ESHV AA+ C++  N  +KIRSGGHDY+G+SY+SD  PFFILD
Sbjct: 79  FNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFILD 138

Query: 130 MFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGGYG 189
           M N+R+++VD  + +AW   GATLGEVYYR+ EK++ +GFPAGVCPTVGVGGH  GGGYG
Sbjct: 139 MSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYG 198

Query: 190 NMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVPV 249
           NM+RK+GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++VPV
Sbjct: 199 NMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPV 258

Query: 250 PETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRATFI 309
           P  VTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RA+ +
Sbjct: 259 PSVVTVFRVEQYMD-SGAVDMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASVV 318

Query: 310 ALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRTPQ 369
           ALFLG ++ ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R   
Sbjct: 319 ALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLD 378

Query: 370 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 429
             +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPHR  
Sbjct: 379 TSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-N 438

Query: 430 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGK 489
            L+KIQY+ NW E   +  K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN +G 
Sbjct: 439 KLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGA 498

Query: 490 NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 499 NSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Chy12G210110 vs. ExPASy Swiss-Prot
Match: Q9SA99 (Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 3.5e-161
Identity = 287/530 (54.15%), Postives = 372/530 (70.19%), Query Frame = 0

Query: 8   ILLLSFSFLVSFSSSSSSAPE----NFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 67
           ++L   S  +S S +    PE    NF QC    +  P   +A  +     A+++ VL  
Sbjct: 6   LILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 65

Query: 68  YIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+AA++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 66  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 125

Query: 128 PFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHF 187
           PFF+LDM NLR ITVD  T++AW   GATLGEVYYR+ EK KS+GFPAGVCPTVG GGH 
Sbjct: 126 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 185

Query: 188 GGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYK 247
            GGGYGNM+RKYGLSVD + DAKI+DVNG++LDRK MGED+FWAI GGGG+SFGV++A+K
Sbjct: 186 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 245

Query: 248 IRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKT 307
           I++VPVP TVTVFRV++ L +N  TE+V +WQ VA   D  LF+R+    V  T    +T
Sbjct: 246 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 305

Query: 308 LRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSV--EAL 367
           +RA+ +ALFLGD   ++ ++   FP+LGLKK +C EM+W+QSV++W N    T +  E L
Sbjct: 306 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 365

Query: 368 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  L FNPYGG M+ + +  TP
Sbjct: 366 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 425

Query: 428 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + G +    F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 426 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 485

Query: 488 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           VN  G NSY    V+G  YF  NFDRLVK+KT VDP NFFR+EQSIP  P
Sbjct: 486 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of Chy12G210110 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 8.0e-158
Identity = 280/528 (53.03%), Postives = 381/528 (72.16%), Query Frame = 0

Query: 6   TSILLLSFSFLVSFSSSSSSAP---ENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLET 65
           T+  L+   FL+   +S ++ P   ++F QC+     PS PI    +T  N ++ ++L  
Sbjct: 5   TTQTLIFTVFLLLIPTSFAAPPKLKDSFTQCVTVFK-PSVPIQNFTYTQQNPNFLTILNN 64

Query: 66  YIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 125
           Y+RNLR+   TT KP  I+ A H +H++A I CA+   LQ++IRSGGHDY+G+SY+S  V
Sbjct: 65  YVRNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLS-TV 124

Query: 126 PFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEK-NKSYGFPAGVCPTVGVGGH 185
            F +LDMFNLR I +D K  TAW Q+GATLGE+YY VA K N   GFPAG+CP +G GGH
Sbjct: 125 DFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGH 184

Query: 186 FGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 245
           F GGGYGNMMRKYGLS+D IIDAKI+D N ++LDR +MGEDLFWA+ GGG +SF VV+A+
Sbjct: 185 FSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAW 244

Query: 246 KIRMVPVPETVTVFRVQRTLEQNDL-TEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 305
           KI++VPVPE VTVF V+    +  + T++  +WQ++A  IDNDLFIR+T    N      
Sbjct: 245 KIKLVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN------ 304

Query: 306 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTS-VEA 365
           KT++A+F+ ++LG+SE+LL ++N  FP+LGL K++CIEM W++SVLFW + P GT+    
Sbjct: 305 KTVKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSV 364

Query: 366 LLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNAT 425
           +L+R PQ   +LKRKSDYV+ PI K GL  I+K + E E   + +NPYGGRM+EIP+  T
Sbjct: 365 MLNRIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATET 424

Query: 426 PFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 485
            FPHRAGN++KIQY++NW   G + A   +  T ++++ M+P+VSKNPR AFLNYRD+DI
Sbjct: 425 AFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDI 484

Query: 486 GVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           G N N  ++Y EG+VYG+KYF+ NF+RLV++KT+VDP N FR EQSIP
Sbjct: 485 GKNLN--STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Chy12G210110 vs. ExPASy TrEMBL
Match: A0A5A7TVZ2 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G001070 PE=3 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 4.1e-298
Identity = 508/532 (95.49%), Postives = 520/532 (97.74%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR+SIL LSFSFLV FSS+SSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLREITVDAKT+TAW QTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Chy12G210110 vs. ExPASy TrEMBL
Match: A0A1S3BE21 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 4.1e-298
Identity = 508/532 (95.49%), Postives = 520/532 (97.74%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR+SIL LSFSFLV FSS+SSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLREITVDAKT+TAW QTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Chy12G210110 vs. ExPASy TrEMBL
Match: A0A6J1K3U3 (berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 7.5e-276
Identity = 471/532 (88.53%), Postives = 499/532 (93.80%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEI R SI LLSFS L+ F +S+++APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSIFLLSFSALL-FFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KT+TAW QTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of Chy12G210110 vs. ExPASy TrEMBL
Match: A0A6J1GSQ1 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.4e-274
Identity = 468/532 (87.97%), Postives = 498/532 (93.61%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+I R S  LLSFS L+ F +S+++APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MDISRFSSFLLSFSALL-FFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KT+TAW QTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKK+DC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYV 533

BLAST of Chy12G210110 vs. ExPASy TrEMBL
Match: A0A6J1DGF3 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.1e-262
Identity = 447/531 (84.18%), Postives = 493/531 (92.84%), Query Frame = 0

Query: 3   ILRTSILL-LSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 62
           ILR   LL L FS L   S++++++PENF +CLLR SPPS+PI+PAIFTP+NAS+SSVL+
Sbjct: 5   ILRPFFLLSLLFSLLFFASATTTASPENFLKCLLRRSPPSHPISPAIFTPDNASFSSVLQ 64

Query: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDV 122
           TYIRNLRFNTS+TPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSDV
Sbjct: 65  TYIRNLRFNTSSTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDV 124

Query: 123 VPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGH 182
           VPFF+LDMFNLR ITVD +T+TAW +TGATLGEVYYR+AEK+K +GFPAGVCPTVGVGGH
Sbjct: 125 VPFFVLDMFNLRAITVDVETETAWVETGATLGEVYYRIAEKSKLHGFPAGVCPTVGVGGH 184

Query: 183 FGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242
           FGGGGYGNMMRKYGLSVDQIIDAK++DVNGKLLDRK+MGEDLFWAI GGGGSSFG VVAY
Sbjct: 185 FGGGGYGNMMRKYGLSVDQIIDAKMVDVNGKLLDRKSMGEDLFWAITGGGGSSFGXVVAY 244

Query: 243 KIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKK 302
           KI+ V VPETVTVF+V+R L +N +TEIVDEWQ+VAH IDNDLFIRVTFDVVNGT  GKK
Sbjct: 245 KIQTVRVPETVTVFKVERRLAEN-VTEIVDEWQRVAHEIDNDLFIRVTFDVVNGTENGKK 304

Query: 303 TLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALL 362
           TLRATFIALFLGDSERLL +++ SFPKLGLK+SDC++MSWL+SVLFWTNFPLGTSV+ALL
Sbjct: 305 TLRATFIALFLGDSERLLSIMDKSFPKLGLKQSDCLQMSWLESVLFWTNFPLGTSVDALL 364

Query: 363 SRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 422
           SR PQVLTHLKRKSDYVKT IPKEGLN+IWKKMIELEKPMLTFNPYGGRMAEI S+ TPF
Sbjct: 365 SRKPQVLTHLKRKSDYVKTLIPKEGLNQIWKKMIELEKPMLTFNPYGGRMAEIASSKTPF 424

Query: 423 PHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGV 482
           PHRAGNLWKIQYATNW EEG +EA HFI+LTRKLY FMTPFVSKNPRTAFLNYRDLDIGV
Sbjct: 425 PHRAGNLWKIQYATNWDEEGVEEADHFINLTRKLYNFMTPFVSKNPRTAFLNYRDLDIGV 484

Query: 483 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           NHNGKNSY EGRV+G+KYFEGNFDRLVKIKT+VDP+NFFRNEQSIPR P+V
Sbjct: 485 NHNGKNSYNEGRVFGVKYFEGNFDRLVKIKTRVDPNNFFRNEQSIPRLPYV 534

BLAST of Chy12G210110 vs. NCBI nr
Match: XP_004135723.1 (berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical protein Csa_023313 [Cucumis sativus])

HSP 1 Score: 1066 bits (2757), Expect = 0.0
Identity = 528/532 (99.25%), Postives = 529/532 (99.44%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEILRTSILLLSFSFLV FSS+SSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFILDMFNLREITVDAKTKTAW QTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK
Sbjct: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Chy12G210110 vs. NCBI nr
Match: XP_008445642.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuline oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1029 bits (2660), Expect = 0.0
Identity = 508/532 (95.49%), Postives = 520/532 (97.74%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR+SIL LSFSFLV FSS+SSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLREITVDAKT+TAW QTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Chy12G210110 vs. NCBI nr
Match: XP_038895093.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 1014 bits (2623), Expect = 0.0
Identity = 499/532 (93.80%), Postives = 517/532 (97.18%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEILR+SILLLSFS+LV FSS++S APENFFQCLLRHSPPSYPIAPAIFTPNN S+SSVL
Sbjct: 1   MEILRSSILLLSFSYLVFFSSATS-APENFFQCLLRHSPPSYPIAPAIFTPNNVSFSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           V+PFFILDMFNLR+ITVD KTKTAW QTGATLGEVYYR+AEKNK YGFPAGVCPTVGVGG
Sbjct: 121 VLPFFILDMFNLRDITVDVKTKTAWVQTGATLGEVYYRIAEKNKFYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPETVTVFRVQRTLEQNDLTEIVDEWQ+VAHVIDNDLFIRVTFDV+NGT KGK
Sbjct: 241 YKIRMVRVPETVTVFRVQRTLEQNDLTEIVDEWQRVAHVIDNDLFIRVTFDVINGTEKGK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATF+A+FLGDSERLL VI  SFPKLGLKKSDC+EMSWLQSVLFWTNFPLGTSV+AL
Sbjct: 301 KTLRATFLAMFLGDSERLLSVIKKSFPKLGLKKSDCLEMSWLQSVLFWTNFPLGTSVDAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGTQEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 531

BLAST of Chy12G210110 vs. NCBI nr
Match: XP_022994779.1 (berberine bridge enzyme-like 8 [Cucurbita maxima])

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 471/532 (88.53%), Postives = 499/532 (93.80%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEI R SI LLSFS L+ F+S+++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSIFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KT+TAW QTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of Chy12G210110 vs. NCBI nr
Match: XP_023542409.1 (berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 471/530 (88.87%), Postives = 497/530 (93.77%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEI R S  LLSFS L+ F+S+++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSSFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KTKTAW QTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTKTAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSKSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 530
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531

BLAST of Chy12G210110 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 686.0 bits (1769), Expect = 2.4e-197
Identity = 340/526 (64.64%), Postives = 410/526 (77.95%), Query Frame = 0

Query: 8   ILLLSFSFLVSFSSSSSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 67
           IL+L F   +  SSSSS+  E F QCL  +S P +PI+PAIF   N SYSSVL+  IRNL
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNL 65

Query: 68  RFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFI 127
           RFNT++TPKPFLI+ A HESHV+AAI C + HNLQMKIRSGGHDY+G+SYV+    PFF+
Sbjct: 66  RFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFV 125

Query: 128 LDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDMFNLR + VD  +KTAW QTGA LGEVYY + EK+K+  +PAG+CPTVGVGGH  GGG
Sbjct: 126 LDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGG 185

Query: 188 YGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNMMRKYGL+VD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AYKI +V
Sbjct: 186 YGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLV 245

Query: 248 PVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRAT 307
            VPE VTVFR+ RTLEQN  T+I+  WQQVA  + ++LFIR   DVVNGT   +KT+R T
Sbjct: 246 EVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 305

Query: 308 FIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALLSRTPQ 367
           FIA+FLGD+  LL ++N  FP+LGL +SDC E SW+QSVLFWTN  +G+S E LL +  Q
Sbjct: 306 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQ 365

Query: 368 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 427
            + +LKRKSDYV+ PI + GL  IWKKMIELE P + FNPYGG M  I S  TPFP+RAG
Sbjct: 366 PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAG 425

Query: 428 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN-HNG 487
           NLWKIQY  NW +E   +   +++LTRKLY+FMTPFVSKNPR +F NYRD+D+G+N HNG
Sbjct: 426 NLWKIQYGANWRDETLTD--RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 488 K-NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           K +SY EG+ YG KYF GNF+RLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Chy12G210110 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 581.3 bits (1497), Expect = 8.2e-166
Identity = 297/523 (56.79%), Postives = 375/523 (71.70%), Query Frame = 0

Query: 15  FLVSFSSSSSSAP-------ENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRN 74
           FLVS    SS+AP       E+F QC   +   P   I   +F+  N S+SSVL  YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 75  LRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFI 134
            RFNTS+TPKP +I+T   + HV AA+ C++  N  +KIRSGGHDYEG+SY+SD  PFFI
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISD-KPFFI 135

Query: 135 LDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 194
           LDM NLR+++VD   ++AW   GATLGEVYYR+ EK+K +GFPAGVCPTVGVGGH  GGG
Sbjct: 136 LDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGG 195

Query: 195 YGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 254
           YGNM+RK+GLSVD +IDAKI+DVNG++LDRK+MGEDLFWAI GGGG+SFGVV+ YK+++V
Sbjct: 196 YGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLV 255

Query: 255 PVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRAT 314
           PVPETVTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RAT
Sbjct: 256 PVPETVTVFRVEKYMD-SGAVDMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRAT 315

Query: 315 FIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRT 374
            +ALFLG +E ++ ++   FP+L LKK +C EM+W QS L+W N    T ++    L R 
Sbjct: 316 VVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRN 375

Query: 375 PQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHR 434
                  KRKSDYV + IP++G+  ++KKM EL K  L FNPYGG+MAE+  NATPFPHR
Sbjct: 376 LDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHR 435

Query: 435 AGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHN 494
           +  L+KIQY+  W E   +  K F++    LY FMT FVSKNPR A+LNYRD+DIGVN +
Sbjct: 436 S-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDH 495

Query: 495 GKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           G NSY EG VYG KYF  NFDRLVK+KT  DP NFFRNEQSIP
Sbjct: 496 GTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Chy12G210110 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 572.0 bits (1473), Expect = 5.0e-163
Identity = 290/521 (55.66%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 10  LLSFSFLVSFSSSSSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLR 69
           LL  S  +S  S S+S   +F +C   +   P   I   +F+  N ++SSVL  YIRN R
Sbjct: 19  LLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNAR 78

Query: 70  FNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFILD 129
           FNTS+T KP +I+T   ESHV AA+ C++  N  +KIRSGGHDY+G+SY+SD  PFFILD
Sbjct: 79  FNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFILD 138

Query: 130 MFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGGYG 189
           M N+R+++VD  + +AW   GATLGEVYYR+ EK++ +GFPAGVCPTVGVGGH  GGGYG
Sbjct: 139 MSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYG 198

Query: 190 NMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVPV 249
           NM+RK+GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++VPV
Sbjct: 199 NMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPV 258

Query: 250 PETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRATFI 309
           P  VTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RA+ +
Sbjct: 259 PSVVTVFRVEQYMD-SGAVDMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASVV 318

Query: 310 ALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRTPQ 369
           ALFLG ++ ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R   
Sbjct: 319 ALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLD 378

Query: 370 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 429
             +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPHR  
Sbjct: 379 TSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-N 438

Query: 430 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGK 489
            L+KIQY+ NW E   +  K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN +G 
Sbjct: 439 KLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGA 498

Query: 490 NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 499 NSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Chy12G210110 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 572.0 bits (1473), Expect = 5.0e-163
Identity = 290/521 (55.66%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 10  LLSFSFLVSFSSSSSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLR 69
           LL  S  +S  S S+S   +F +C   +   P   I   +F+  N ++SSVL  YIRN R
Sbjct: 19  LLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNAR 78

Query: 70  FNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFILD 129
           FNTS+T KP +I+T   ESHV AA+ C++  N  +KIRSGGHDY+G+SY+SD  PFFILD
Sbjct: 79  FNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFILD 138

Query: 130 MFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGGYG 189
           M N+R+++VD  + +AW   GATLGEVYYR+ EK++ +GFPAGVCPTVGVGGH  GGGYG
Sbjct: 139 MSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYG 198

Query: 190 NMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVPV 249
           NM+RK+GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++VPV
Sbjct: 199 NMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPV 258

Query: 250 PETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRATFI 309
           P  VTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RA+ +
Sbjct: 259 PSVVTVFRVEQYMD-SGAVDMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASVV 318

Query: 310 ALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRTPQ 369
           ALFLG ++ ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R   
Sbjct: 319 ALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLD 378

Query: 370 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 429
             +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPHR  
Sbjct: 379 TSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-N 438

Query: 430 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGK 489
            L+KIQY+ NW E   +  K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN +G 
Sbjct: 439 KLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGA 498

Query: 490 NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 499 NSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Chy12G210110 vs. TAIR 10
Match: AT1G11770.1 (FAD-binding Berberine family protein )

HSP 1 Score: 569.7 bits (1467), Expect = 2.5e-162
Identity = 287/530 (54.15%), Postives = 372/530 (70.19%), Query Frame = 0

Query: 8   ILLLSFSFLVSFSSSSSSAPE----NFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 67
           ++L   S  +S S +    PE    NF QC    +  P   +A  +     A+++ VL  
Sbjct: 6   LILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 65

Query: 68  YIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+AA++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 66  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 125

Query: 128 PFFILDMFNLREITVDAKTKTAWAQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHF 187
           PFF+LDM NLR ITVD  T++AW   GATLGEVYYR+ EK KS+GFPAGVCPTVG GGH 
Sbjct: 126 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 185

Query: 188 GGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYK 247
            GGGYGNM+RKYGLSVD + DAKI+DVNG++LDRK MGED+FWAI GGGG+SFGV++A+K
Sbjct: 186 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 245

Query: 248 IRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKT 307
           I++VPVP TVTVFRV++ L +N  TE+V +WQ VA   D  LF+R+    V  T    +T
Sbjct: 246 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 305

Query: 308 LRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSV--EAL 367
           +RA+ +ALFLGD   ++ ++   FP+LGLKK +C EM+W+QSV++W N    T +  E L
Sbjct: 306 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 365

Query: 368 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  L FNPYGG M+ + +  TP
Sbjct: 366 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 425

Query: 428 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + G +    F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 426 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 485

Query: 488 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           VN  G NSY    V+G  YF  NFDRLVK+KT VDP NFFR+EQSIP  P
Sbjct: 486 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530