Chy12G210100 (gene) Cucumber (hystrix) v1

BLAST of Chy12G210100 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 3.1e-152
Identity = 270/527 (51.23%), Postives = 371/527 (70.40%), Query Frame = 0

Query: 13  FCVSLIWLSHS----SSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNE--SASFSSLLRL 72
           F V+L+ LS S    S  ++Q +F+ C      S  S P+    FT++  ++SF   L  
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCL--VDNSDASFPMDSSFFTHDLNASSFKLALET 71

Query: 73  SIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYISS-Q 132
           S +NLR+L  + PKP  +  P +E+HVQAA++CA++  L +R+RSGGHDYEGLSY+S  +
Sbjct: 72  SAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEME 131

Query: 133 APFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGH 192
             F+I+DL  LR I++DI++ +A V  GA++GE+YYRI +KS IHGFPAG C ++G+GGH
Sbjct: 132 TAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGH 191

Query: 193 ISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSW 252
           I GG +G++ RK+GL ADNV+DA+I+D +G+I++R++MGED+FWAIRGGGG SFGVIL+W
Sbjct: 192 IIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAW 251

Query: 253 KLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKK-- 312
           K+KLV VP IVTVF V RTLEQ  T L  KWQ ++ KLD+D+F+ V  +  +  P  K  
Sbjct: 252 KIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKER 311

Query: 313 TIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLEVLM 372
           TI  S+   FLG   +L+ +M+  F +LGLT+ DC+E SWI+SV++ A F    P E L+
Sbjct: 312 TISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALL 371

Query: 373 ERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQT 432
           +  S  +   +F AKSDYV  PI   GLEGLW KL+EE+    I+ PYGG M++I E++T
Sbjct: 372 DGKS--LFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETET 431

Query: 433 PFPHREGRVFGIQYLATWDNANETE-KHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG 492
           PFPHR G +F IQ+L  W +   +E KH+ W+RE+Y+YME YVSKSPR+AY+NYRDLDLG
Sbjct: 432 PFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLG 491

Query: 493 TNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 530
            N G+ +   +A+ WG +YF  NFERLV +K K DP NFF +EQSIP
Sbjct: 492 MN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Chy12G210100 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.2e-151
Identity = 263/527 (49.91%), Postives = 367/527 (69.64%), Query Frame = 0

Query: 9   LFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLLRLSI 68
           + +LF V  IW S SSS +  + F +C  S S  KH  P+S  +F + + S+SS+L+ +I
Sbjct: 6   ILVLFFVVFIWQS-SSSSANSETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANI 65

Query: 69  RNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYIS-SQAP 128
           RNLRF  T+ PKP L++   HESHVQAAI C +   LQ+++RSGGHDY+GLSY++ S  P
Sbjct: 66  RNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKP 125

Query: 129 FIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHIS 188
           F ++D+ NLRS+ +D+ ++TA V+TGA LGE+YY I +KS    +PAG CPTVGVGGHIS
Sbjct: 126 FFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHIS 185

Query: 189 GGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKL 248
           GGG+G + RKYGL  DN IDA+++D NG+I+DR  MGEDL+WAI GGGG S+GV+L++K+
Sbjct: 186 GGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKI 245

Query: 249 KLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVT-NFPSKKTIR 308
            LV VP  VTVF + RTLEQ AT +  +WQ ++ KL  ++F+     VV     S+KT+R
Sbjct: 246 NLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVR 305

Query: 309 LSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLEVLMERS 368
            +F ++FLG    L+ I+  RF ELGL R+DC E SWIQSVLF+ +  + +   +L++R+
Sbjct: 306 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN 365

Query: 369 SPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQTPFP 428
            P     +   KSDYV  PIS  GLE +W K++E E   + F PYGG+M +IS + TPFP
Sbjct: 366 QPV---NYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFP 425

Query: 429 HREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTN-- 488
           +R G ++ IQY A W +   T++++   R++Y +M  +VSK+PR ++ NYRD+DLG N  
Sbjct: 426 YRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSH 485

Query: 489 YGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLL 532
            G+ +SY E K +G KYF+ NFERLV++KT+VD  NFF NEQSIP+L
Sbjct: 486 NGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Chy12G210100 vs. ExPASy Swiss-Prot
Match: Q9FKV2 (Berberine bridge enzyme-like 23 OS=Arabidopsis thaliana OX=3702 GN=At5g44360 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.9e-150
Identity = 276/537 (51.40%), Postives = 371/537 (69.09%), Query Frame = 0

Query: 7   IHLFLLFCVSLIWL-SHSSSDSLQDNFLECFNSTSYSKHSIPVSEV------VFTNESAS 66
           + LFL+F V   W+ S SSS   +D FL C ++ S S    P S +      V+T+ S S
Sbjct: 9   LSLFLVFLVK--WVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQS 68

Query: 67  FSSLLRLSIRNLRFLAT--TLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYE 126
             S      +N RFL    T  KP+L+VTP  ++ +Q +++C+R+ G++VR +SGGHDYE
Sbjct: 69  LIS------QNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYE 128

Query: 127 GLSYISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSC 186
           GLSY+S  +PFII+DL+N+RSI I++  ETA V  GA +GELYY+IAK S IHGFPAG+C
Sbjct: 129 GLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTC 188

Query: 187 PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMD-RSSMGEDLFWAIRGGGG 246
           P+VGVGGH SGGGFG + RK+GLAADNV+DA+ +D NGRI + R  MGEDLFWAIRGGG 
Sbjct: 189 PSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGA 248

Query: 247 ASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVV 306
           ASFGV+LSWK+KLV VP  VT F     L Q  T +  +WQ I+ +LD ++F+ V   + 
Sbjct: 249 ASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSI- 308

Query: 307 TNFPSKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSID 366
               S  +++ +F + +LG I+KLIP+M  +F ELGLT  DC EM+WI S+++F ++   
Sbjct: 309 ----SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF-NWKKG 368

Query: 367 TPLEVLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMS 426
            PLE L++R   + +D +F AKSD+V +PI E GLEG+W++  E E   +I  P GGKM 
Sbjct: 369 QPLETLLDRGQ-RYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLGGKMY 428

Query: 427 QISESQTPFPHREGRVFGIQYLATW--DNANETEKHLSWIREVYAYMESYVSKSPRAAYL 486
           +I E++TPFPHR G ++ IQY+  W   +    EKH++W+R +Y YM  YVS SPR AYL
Sbjct: 429 EIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYL 488

Query: 487 NYRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLL 532
           NYRDLDLG N G NTS+E+AK+WG +YF  NF+RL  VK K+DP+NFF NEQS+P L
Sbjct: 489 NYRDLDLGMNRGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPL 530

BLAST of Chy12G210100 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.4e-149
Identity = 268/531 (50.47%), Postives = 367/531 (69.11%), Query Frame = 0

Query: 7   IHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLLRL 66
           I +F L+  S   ++ +SS SLQD+F++C    +  + ++  +       ++ F+ +L  
Sbjct: 13  ISVFALY-FSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLES 72

Query: 67  SIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYISS-Q 126
           + +N R+L  T+PKP  +  P HESHVQA+++C+++  +  RVRSGGHDYEG+SY+S  +
Sbjct: 73  TAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIE 132

Query: 127 APFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGH 186
            PF++IDL  LR I +DIK  +A VE GA +GELYYRIA+KS  HGFPAG  P++G+GGH
Sbjct: 133 KPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGH 192

Query: 187 ISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSW 246
           I+GG +G+L RKYGLAADNV+DAKI+D NG+++DR+SMGEDLFWAIRGG G SFG+ILSW
Sbjct: 193 ITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSW 252

Query: 247 KLKLVSVPSIVTVFNVQRTLEQGAT-HLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKKT 306
           K+KLV VP  +TVF V +T EQ  +  +  KWQ I+  L  ++FL V   V  N  + KT
Sbjct: 253 KIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN-KANKT 312

Query: 307 IRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSID--TPLEVL 366
           + +++   FLG    L+ +MK  F ELGLT+ DCIEMSWI S+++ + F  +   P+E+L
Sbjct: 313 VTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEIL 372

Query: 367 MERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQ 426
           ++  SP I   +F  KSD+   PI   GLEG++ KL+EE+ + +I+TPYGGKM +I ES+
Sbjct: 373 LQAKSP-IGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESE 432

Query: 427 TPFPHREGRVFGIQYLATW-DNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDL 486
            PFPHR G  F IQY  +W D+     +   WIRE+Y YM  YVS +PR AY+NYRDLDL
Sbjct: 433 IPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDL 492

Query: 487 GTNYGRNTS-YEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLL 532
           G N   + S + EAK+WG  YF DNF RLVR+K+KVDP NFF +EQSIP L
Sbjct: 493 GQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of Chy12G210100 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 4.2e-149
Identity = 261/523 (49.90%), Postives = 359/523 (68.64%), Query Frame = 0

Query: 12  LFCVSLIWLS-HSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLLRLSIRN 71
           LF V+L+ +S   SS +LQ +F++C    S     I  S       +  F   L  + +N
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 72  LRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYIS-SQAPFI 131
           LR+L  + PKP+ +  P +E+HVQAA+VCA++  L +R+RSGGHDYEGLS+++  + PF+
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 131

Query: 132 IIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGG 191
           I+DL  LR + +D+ + +A    GA +GE+YYRI +KS  HGFPAG C ++G+GGH+ GG
Sbjct: 132 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 191

Query: 192 GFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKL 251
            +G++ RK+GL ADNV+DA+I+D NG+I+DR++MGED+FWAIRGGGG SFGVIL+WK+KL
Sbjct: 192 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 251

Query: 252 VSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVV--TNFPSKKTIRL 311
           V VP+ VTVF V +TLEQ  T +  KW+ I+ KLD D+F+ V       T  P  +TI +
Sbjct: 252 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISM 311

Query: 312 SFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLEVLMERSS 371
           S+ + FLG   +L+ +M+  F ELGLT+ DC EMSWI+SV++ A F      E L+   S
Sbjct: 312 SYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKS 371

Query: 372 PQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQTPFPH 431
             +    F AKSD+V  PI   GLEGLW + +EE+    I+ PYGG MS+ISES+ PFPH
Sbjct: 372 --LFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPH 431

Query: 432 REGRVFGIQYLATW-DNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYG 491
           R G +F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLGTN G
Sbjct: 432 RNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEG 491

Query: 492 RNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 530
                 +A+ WG KY+  NFERLV++K + DP NFF +EQS+P
Sbjct: 492 ET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Chy12G210100 vs. ExPASy TrEMBL
Match: A0A5A7TYX1 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00060 PE=3 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 8.0e-305
Identity = 526/555 (94.77%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 60
           MICLRR HLFLLFCVSLIWL  SSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF
Sbjct: 3   MICLRRTHLFLLFCVSLIWLC-SSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 62

Query: 61  SSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 120
           SSL RLSIRNLRFL TTLPKPL LVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS
Sbjct: 63  SSLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 122

Query: 121 YISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTV 180
           Y+SSQAPFI+IDLINLRSITIDI+ ETASV TGAALGELYYRIAKKSSIHGFPAGSCPTV
Sbjct: 123 YVSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTV 182

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG
Sbjct: 183 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 242

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVT+FP
Sbjct: 243 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFP 302

Query: 301 SKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLE 360
           SKKTIRLSFTSLFLGPIE+LIPIMKTRFSELGL R+DCIEMSWIQSVLFFADFSIDTPLE
Sbjct: 303 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLE 362

Query: 361 VLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISE 420
           VLMERSSPQISDAFFTAKSDYV SPIS+ GLEGLW KL+EEEKSELI TPYGGKMSQISE
Sbjct: 363 VLMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISE 422

Query: 421 SQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQTPFPHREGR+FGIQYLATWDNANETEKHLSWIREVYAYM+ YVSKSPRAAYLNYRDLD
Sbjct: 423 SQTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLD 482

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDIKI 540
           LG NYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD +I
Sbjct: 483 LGRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRI 542

Query: 541 TKVHSGLDFKIIQER 556
           TKVHSGLDF+I+QER
Sbjct: 543 TKVHSGLDFEIVQER 556

BLAST of Chy12G210100 vs. ExPASy TrEMBL
Match: A0A1S3BE11 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103488588 PE=3 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 8.0e-305
Identity = 526/555 (94.77%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 60
           MICLRR HLFLLFCVSLIWL  SSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF
Sbjct: 3   MICLRRTHLFLLFCVSLIWLC-SSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 62

Query: 61  SSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 120
           SSL RLSIRNLRFL TTLPKPL LVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS
Sbjct: 63  SSLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 122

Query: 121 YISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTV 180
           Y+SSQAPFI+IDLINLRSITIDI+ ETASV TGAALGELYYRIAKKSSIHGFPAGSCPTV
Sbjct: 123 YVSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTV 182

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG
Sbjct: 183 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 242

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVT+FP
Sbjct: 243 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFP 302

Query: 301 SKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLE 360
           SKKTIRLSFTSLFLGPIE+LIPIMKTRFSELGL R+DCIEMSWIQSVLFFADFSIDTPLE
Sbjct: 303 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLE 362

Query: 361 VLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISE 420
           VLMERSSPQISDAFFTAKSDYV SPIS+ GLEGLW KL+EEEKSELI TPYGGKMSQISE
Sbjct: 363 VLMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISE 422

Query: 421 SQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQTPFPHREGR+FGIQYLATWDNANETEKHLSWIREVYAYM+ YVSKSPRAAYLNYRDLD
Sbjct: 423 SQTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLD 482

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDIKI 540
           LG NYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD +I
Sbjct: 483 LGRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRI 542

Query: 541 TKVHSGLDFKIIQER 556
           TKVHSGLDF+I+QER
Sbjct: 543 TKVHSGLDFEIVQER 556

BLAST of Chy12G210100 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.5e-253
Identity = 441/561 (78.61%), Postives = 489/561 (87.17%), Query Frame = 0

Query: 6   RIHLFLLF--CVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSL 65
           RIHLFL+F  CV       SSSD LQ++F+ECFNST+YSKHS+P+SEVVFTNESASFSSL
Sbjct: 8   RIHLFLIFFHCV-------SSSDCLQESFMECFNSTTYSKHSVPISEVVFTNESASFSSL 67

Query: 66  LRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYIS 125
           LRLSIRNLRFL  + PKP  LVTPF  SHVQAA+VCA++K LQVRVRSGGHDYEGLSY+S
Sbjct: 68  LRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYEGLSYVS 127

Query: 126 SQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVG 185
           SQ PFI+IDLINL SI IDI+TETASVETGA LGE+YYRIA+KS IHGFPAGSCPTVGVG
Sbjct: 128 SQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSCPTVGVG 187

Query: 186 GHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVIL 245
           GH+SGGGFGTLFRKYGLAADNVIDAKI+DFNGRI DR+SMGEDLFWAIRGGGGASFGVIL
Sbjct: 188 GHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGASFGVIL 247

Query: 246 SWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVV------- 305
           SWKLKLVSVP  VTVFNV RTL+QGATHLFQKWQ I+HK+DQD+FLHVTTKVV       
Sbjct: 248 SWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLS 307

Query: 306 -TNFPSKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSI 365
            +   S KTI+LSFTSLFLGPIE+LIP+M T F ELGL RNDC EMSWIQSVLFFAD S 
Sbjct: 308 SSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLFFADLST 367

Query: 366 DTPLEVLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKM 425
           +TP E+LMER SP ISD FFTAKSDYVTSPIS+ GLE LWSKL+EEEKSELI TPYGGKM
Sbjct: 368 ETPPEILMER-SPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTPYGGKM 427

Query: 426 SQISESQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLN 485
            QISES+TPFPHR G ++GIQYLA W+NANETEKHLSW+REVY+YM+ +VSK PR AYLN
Sbjct: 428 DQISESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPRGAYLN 487

Query: 486 YRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYE 545
           YRDLDLG NY RNTSY+EAKVWGLKYF  NFERLVRVKTKVDP NFFWNEQSIPL +H+E
Sbjct: 488 YRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCHHHE 547

Query: 546 DD-IKITKVHSGLDFKIIQER 556
           ++  +I +V+S LD KI+QER
Sbjct: 548 ENGTRILQVNSYLDSKILQER 560

BLAST of Chy12G210100 vs. ExPASy TrEMBL
Match: A0A6J1JUS4 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111489931 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 9.0e-248
Identity = 434/560 (77.50%), Postives = 486/560 (86.79%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWL-SHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESAS 60
           M+  RRI L L F V LIW+ S SSSD  Q+NFLECFNST+YSKHS+PVSEVVF+NESAS
Sbjct: 1   MVHWRRIQLLLFFWVPLIWICSSSSSDFDQENFLECFNSTTYSKHSLPVSEVVFSNESAS 60

Query: 61  FSSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGL 120
           FSSLL+ SIRNLRFL+T+ PKPL LVTPFHESHVQAAIVCA+E  LQVRV SGGHDYEGL
Sbjct: 61  FSSLLKHSIRNLRFLSTSSPKPLFLVTPFHESHVQAAIVCAKENDLQVRVLSGGHDYEGL 120

Query: 121 SYI-SSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCP 180
           SY+ SSQAPFI+IDLINLRSI I+IKTETA VETGA +GELYYRIAKKS IHGFPAGSCP
Sbjct: 121 SYLSSSQAPFIVIDLINLRSIDINIKTETALVETGATIGELYYRIAKKSPIHGFPAGSCP 180

Query: 181 TVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGAS 240
           TVGVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR++MGEDLFWAIRGGGGAS
Sbjct: 181 TVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNTMGEDLFWAIRGGGGAS 240

Query: 241 FGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTN 300
           FGVILSWKLKLVS+PSIVT FNVQRTL QGATHLFQKWQ I+HKLDQD+FLHVTTKV+  
Sbjct: 241 FGVILSWKLKLVSLPSIVTTFNVQRTLNQGATHLFQKWQQIAHKLDQDLFLHVTTKVIDQ 300

Query: 301 FPSK---KTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSI 360
             +K   KT+ LSFTSLFLG I+ LIP+M T F ELGL R  C EM+WIQS+LFFADFS 
Sbjct: 301 KMTKTTSKTLSLSFTSLFLGSIDSLIPLMDTHFPELGLKRYQCTEMNWIQSILFFADFST 360

Query: 361 DTPLEVLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKM 420
            +PLE+LM+R     S   F AKSDYVTSPIS++GLEGLW+KL+E E SELIFTPYGGKM
Sbjct: 361 KSPLEILMQRLPSTRSS--FIAKSDYVTSPISQSGLEGLWAKLLENENSELIFTPYGGKM 420

Query: 421 SQISESQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLN 480
           SQISE +TPFPHR+G +FG+QYLATWD++NE E+HL WIREVY+YME YVS +PR  YLN
Sbjct: 421 SQISELETPFPHRKGSIFGVQYLATWDDSNENERHLRWIREVYSYMEPYVSMAPRGTYLN 480

Query: 481 YRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYE 540
           YRDLDLG NYGRNTSYEEAKVWGLKYF  NFERLV+VKTK DP NFFWNEQSIPLLY+ E
Sbjct: 481 YRDLDLGRNYGRNTSYEEAKVWGLKYFKGNFERLVKVKTKFDPLNFFWNEQSIPLLYNCE 540

Query: 541 DDIKITKVHSGLDFKIIQER 556
           D+ +I +V+S L+ + +QER
Sbjct: 541 DEARICEVYSDLNSENVQER 558

BLAST of Chy12G210100 vs. ExPASy TrEMBL
Match: A0A6J1GQY4 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111456716 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 7.4e-242
Identity = 427/559 (76.39%), Postives = 481/559 (86.05%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 60
           M+  RRI L L F +     S SSS  +Q+NF+ECFNST+YSKHS+PVSEVVF+NESASF
Sbjct: 1   MVHWRRIQLLLFFWICSSSCS-SSSAFVQENFIECFNSTTYSKHSLPVSEVVFSNESASF 60

Query: 61  SSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 120
           SSLL+ SIRNLRFL+T+ PKPL LVTPFHESHVQAAIVCA+E  LQVRV SGGHDYEGLS
Sbjct: 61  SSLLKHSIRNLRFLSTSSPKPLFLVTPFHESHVQAAIVCAKENDLQVRVLSGGHDYEGLS 120

Query: 121 YI-SSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPT 180
           Y+ SSQAPFI+IDLINLRSI I+I+TETASVETGA LGELYYRIAKKS IHGFPAGSCPT
Sbjct: 121 YLSSSQAPFIVIDLINLRSIDINIETETASVETGATLGELYYRIAKKSPIHGFPAGSCPT 180

Query: 181 VGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASF 240
           VGVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR++M EDLFWAIRGGGGASF
Sbjct: 181 VGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNTMEEDLFWAIRGGGGASF 240

Query: 241 GVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNF 300
           GVILSWKLKLVS+PSIVT FNVQRTL QG THLF KWQ I+HKL+QD+FLHVTTKV+   
Sbjct: 241 GVILSWKLKLVSLPSIVTTFNVQRTLNQGGTHLFLKWQEIAHKLNQDLFLHVTTKVIDQK 300

Query: 301 PSK---KTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSID 360
            +K   KT+ LSFTSLFLG I+ LIP+M T F ELGL R  C EM+WIQSVLFFADFS  
Sbjct: 301 MTKTTSKTLSLSFTSLFLGSIDTLIPLMDTHFPELGLKRYQCTEMNWIQSVLFFADFSTK 360

Query: 361 TPLEVLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMS 420
           +PLE+LM+R  P I  +F  AKSDYVTSPIS++GLEGLW+KL+E + SELIFTPYGGKMS
Sbjct: 361 SPLEILMQR-LPSIRSSFI-AKSDYVTSPISQSGLEGLWAKLLENDNSELIFTPYGGKMS 420

Query: 421 QISESQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNY 480
           QISE +TPFPHR+G +FG+QYLATWD++NE E+HL WIREVY+YME YVS +PR  YLNY
Sbjct: 421 QISELETPFPHRKGSIFGVQYLATWDDSNENERHLRWIREVYSYMEPYVSMAPRGTYLNY 480

Query: 481 RDLDLGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYED 540
           RDLDLG NYGRNTSYEEAKVWGLKYF  NFERLV+VKTK DP NFFWNEQSIPLLY+ ED
Sbjct: 481 RDLDLGRNYGRNTSYEEAKVWGLKYFKGNFERLVKVKTKFDPLNFFWNEQSIPLLYNCED 540

Query: 541 DIKITKVHSGLDFKIIQER 556
           + +I +V+S LD   +QER
Sbjct: 541 EARICEVYSDLDSANVQER 556

BLAST of Chy12G210100 vs. NCBI nr
Match: KAE8653305.1 (hypothetical protein Csa_023319 [Cucumis sativus])

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 531/555 (95.68%), Postives = 544/555 (98.02%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 60
           MIC RRIHLFLL CVSLIWL  SSSDSLQDNFLECFNSTSYSKHSIP+SEVVFTNESASF
Sbjct: 3   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 62

Query: 61  SSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 120
           SSLLRLSIRNLRFL TTLPKPLLLVTPFHESHVQAAI+CAREKGLQVRVRSGGHDYEGLS
Sbjct: 63  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 122

Query: 121 YISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTV 180
           YISSQAPFI+IDLINLRSI IDIKTETASVETGA+LGELYYRIAKKSSIHGFPAGSCPTV
Sbjct: 123 YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV 182

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG
Sbjct: 183 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 242

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLE+GATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP
Sbjct: 243 VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 302

Query: 301 SKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLE 360
           SKKTIRLSFTSLFLGPIE+LIPIMKTRFSELGL RNDCIEMSWIQSVLFFADFSID PLE
Sbjct: 303 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE 362

Query: 361 VLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISE 420
           VLM+RSSPQISDAFFTAKSDYVTSPISENGLEGLWSKL+EE+KSELIFTPYGGKMSQISE
Sbjct: 363 VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE 422

Query: 421 SQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQ PFPHREGR+FGIQYLATWDNANE EKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD
Sbjct: 423 SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 482

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDIKI 540
           LGTNYGRNTSYEEAKVWGLKYFSDNF+RLVRVKTKVDPSNFFWNEQSIPLLYHYEDD K+
Sbjct: 483 LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV 542

Query: 541 TKVHSGLDFKIIQER 555
           TKVHSGLDF+IIQER
Sbjct: 543 TKVHSGLDFEIIQER 557

BLAST of Chy12G210100 vs. NCBI nr
Match: XP_004135724.1 (berberine bridge enzyme-like 13 [Cucumis sativus])

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 531/555 (95.68%), Postives = 544/555 (98.02%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 60
           MIC RRIHLFLL CVSLIWL  SSSDSLQDNFLECFNSTSYSKHSIP+SEVVFTNESASF
Sbjct: 1   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 60

Query: 61  SSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 120
           SSLLRLSIRNLRFL TTLPKPLLLVTPFHESHVQAAI+CAREKGLQVRVRSGGHDYEGLS
Sbjct: 61  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 120

Query: 121 YISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTV 180
           YISSQAPFI+IDLINLRSI IDIKTETASVETGA+LGELYYRIAKKSSIHGFPAGSCPTV
Sbjct: 121 YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV 180

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLE+GATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP
Sbjct: 241 VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300

Query: 301 SKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLE 360
           SKKTIRLSFTSLFLGPIE+LIPIMKTRFSELGL RNDCIEMSWIQSVLFFADFSID PLE
Sbjct: 301 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE 360

Query: 361 VLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISE 420
           VLM+RSSPQISDAFFTAKSDYVTSPISENGLEGLWSKL+EE+KSELIFTPYGGKMSQISE
Sbjct: 361 VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE 420

Query: 421 SQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQ PFPHREGR+FGIQYLATWDNANE EKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD
Sbjct: 421 SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDIKI 540
           LGTNYGRNTSYEEAKVWGLKYFSDNF+RLVRVKTKVDPSNFFWNEQSIPLLYHYEDD K+
Sbjct: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV 540

Query: 541 TKVHSGLDFKIIQER 555
           TKVHSGLDF+IIQER
Sbjct: 541 TKVHSGLDFEIIQER 555

BLAST of Chy12G210100 vs. NCBI nr
Match: XP_008445632.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0047127.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa] >TYK14944.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1048 bits (2711), Expect = 0.0
Identity = 526/555 (94.77%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 60
           MICLRR HLFLLFCVSLIWL  SSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF
Sbjct: 3   MICLRRTHLFLLFCVSLIWLC-SSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 62

Query: 61  SSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 120
           SSL RLSIRNLRFL TTLPKPL LVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS
Sbjct: 63  SSLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 122

Query: 121 YISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTV 180
           Y+SSQAPFI+IDLINLRSITIDI+ ETASV TGAALGELYYRIAKKSSIHGFPAGSCPTV
Sbjct: 123 YVSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTV 182

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG
Sbjct: 183 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 242

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVT+FP
Sbjct: 243 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFP 302

Query: 301 SKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLE 360
           SKKTIRLSFTSLFLGPIE+LIPIMKTRFSELGL R+DCIEMSWIQSVLFFADFSIDTPLE
Sbjct: 303 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLE 362

Query: 361 VLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISE 420
           VLMERSSPQISDAFFTAKSDYV SPIS+ GLEGLW KL+EEEKSELI TPYGGKMSQISE
Sbjct: 363 VLMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISE 422

Query: 421 SQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQTPFPHREGR+FGIQYLATWDNANETEKHLSWIREVYAYM+ YVSKSPRAAYLNYRDLD
Sbjct: 423 SQTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLD 482

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDIKI 540
           LG NYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD +I
Sbjct: 483 LGRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRI 542

Query: 541 TKVHSGLDFKIIQER 555
           TKVHSGLDF+I+QER
Sbjct: 543 TKVHSGLDFEIVQER 556

BLAST of Chy12G210100 vs. NCBI nr
Match: XP_038895849.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 1014 bits (2621), Expect = 0.0
Identity = 502/555 (90.45%), Postives = 530/555 (95.50%), Query Frame = 0

Query: 1   MICLRRIHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 60
           M+CLRR+HLFLLF VSLIWL  SSSDSLQ+ FLECFNSTSYSKHSIP+SEVVFTNESASF
Sbjct: 1   MLCLRRVHLFLLFFVSLIWLC-SSSDSLQERFLECFNSTSYSKHSIPISEVVFTNESASF 60

Query: 61  SSLLRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 120
           SSLL+LSIRNLRFL TT+PKPL LVTPFHESHVQAAIVCAR+KGLQVRVRSGGHDYEGLS
Sbjct: 61  SSLLQLSIRNLRFLTTTMPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLS 120

Query: 121 YISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTV 180
           Y+SSQAPFI+IDLINL SITIDI+TET SVETGA LGELYY+IA+KS IHGFPAGSCPTV
Sbjct: 121 YVSSQAPFIVIDLINLHSITIDIETETTSVETGATLGELYYKIAEKSPIHGFPAGSCPTV 180

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGH+SGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFG 240

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQ I+HKLDQDIFLHVTTKV+TNF 
Sbjct: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQTIAHKLDQDIFLHVTTKVITNFS 300

Query: 301 SKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLE 360
           SKKTIRLSFTSLFLGPIE+LIPIMKT FSELGL RNDC EMSWIQSVLFFADFSI+TPLE
Sbjct: 301 SKKTIRLSFTSLFLGPIERLIPIMKTHFSELGLKRNDCTEMSWIQSVLFFADFSIETPLE 360

Query: 361 VLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISE 420
           VLMERSSP ISDAFFTAKSDYVTSPIS+NGLEGLW+KL+EEEKSELI TPYGGKMSQISE
Sbjct: 361 VLMERSSPPISDAFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISE 420

Query: 421 SQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQTPFPHR G +FGIQYLATWDNANETEKHLSWIREVY YME YVSKSPRAAYLNYRDLD
Sbjct: 421 SQTPFPHRRGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLD 480

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDIKI 540
           LG NY +NTSYEEAKVWGLKYFSDNFERLVRVKTKVDP NFFWNEQSIPLLYHYEDD +I
Sbjct: 481 LGRNYRKNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPLNFFWNEQSIPLLYHYEDDTRI 540

Query: 541 TKVHSGLDFKIIQER 555
           +KVHSGLDF+I+QER
Sbjct: 541 SKVHSGLDFEIVQER 554

BLAST of Chy12G210100 vs. NCBI nr
Match: XP_022140178.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 441/561 (78.61%), Postives = 489/561 (87.17%), Query Frame = 0

Query: 6   RIHLFLLF--CVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSL 65
           RIHLFL+F  CVS       SSD LQ++F+ECFNST+YSKHS+P+SEVVFTNESASFSSL
Sbjct: 8   RIHLFLIFFHCVS-------SSDCLQESFMECFNSTTYSKHSVPISEVVFTNESASFSSL 67

Query: 66  LRLSIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYIS 125
           LRLSIRNLRFL  + PKP  LVTPF  SHVQAA+VCA++K LQVRVRSGGHDYEGLSY+S
Sbjct: 68  LRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYEGLSYVS 127

Query: 126 SQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVG 185
           SQ PFI+IDLINL SI IDI+TETASVETGA LGE+YYRIA+KS IHGFPAGSCPTVGVG
Sbjct: 128 SQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSCPTVGVG 187

Query: 186 GHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVIL 245
           GH+SGGGFGTLFRKYGLAADNVIDAKI+DFNGRI DR+SMGEDLFWAIRGGGGASFGVIL
Sbjct: 188 GHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGASFGVIL 247

Query: 246 SWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVV------- 305
           SWKLKLVSVP  VTVFNV RTL+QGATHLFQKWQ I+HK+DQD+FLHVTTKVV       
Sbjct: 248 SWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLS 307

Query: 306 -TNFPSKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSI 365
            +   S KTI+LSFTSLFLGPIE+LIP+M T F ELGL RNDC EMSWIQSVLFFAD S 
Sbjct: 308 SSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLFFADLST 367

Query: 366 DTPLEVLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKM 425
           +TP E+LMERS P ISD FFTAKSDYVTSPIS+ GLE LWSKL+EEEKSELI TPYGGKM
Sbjct: 368 ETPPEILMERSPP-ISDTFFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTPYGGKM 427

Query: 426 SQISESQTPFPHREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLN 485
            QISES+TPFPHR G ++GIQYLA W+NANETEKHLSW+REVY+YM+ +VSK PR AYLN
Sbjct: 428 DQISESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPRGAYLN 487

Query: 486 YRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYE 545
           YRDLDLG NY RNTSY+EAKVWGLKYF  NFERLVRVKTKVDP NFFWNEQSIPL +H+E
Sbjct: 488 YRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCHHHE 547

Query: 546 DD-IKITKVHSGLDFKIIQER 555
           ++  +I +V+S LD KI+QER
Sbjct: 548 ENGTRILQVNSYLDSKILQER 560

BLAST of Chy12G210100 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 540.0 bits (1390), Expect = 2.2e-153
Identity = 270/527 (51.23%), Postives = 371/527 (70.40%), Query Frame = 0

Query: 13  FCVSLIWLSHS----SSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNE--SASFSSLLRL 72
           F V+L+ LS S    S  ++Q +F+ C      S  S P+    FT++  ++SF   L  
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCL--VDNSDASFPMDSSFFTHDLNASSFKLALET 71

Query: 73  SIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYISS-Q 132
           S +NLR+L  + PKP  +  P +E+HVQAA++CA++  L +R+RSGGHDYEGLSY+S  +
Sbjct: 72  SAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEME 131

Query: 133 APFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGH 192
             F+I+DL  LR I++DI++ +A V  GA++GE+YYRI +KS IHGFPAG C ++G+GGH
Sbjct: 132 TAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGH 191

Query: 193 ISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSW 252
           I GG +G++ RK+GL ADNV+DA+I+D +G+I++R++MGED+FWAIRGGGG SFGVIL+W
Sbjct: 192 IIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAW 251

Query: 253 KLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKK-- 312
           K+KLV VP IVTVF V RTLEQ  T L  KWQ ++ KLD+D+F+ V  +  +  P  K  
Sbjct: 252 KIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKER 311

Query: 313 TIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLEVLM 372
           TI  S+   FLG   +L+ +M+  F +LGLT+ DC+E SWI+SV++ A F    P E L+
Sbjct: 312 TISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALL 371

Query: 373 ERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQT 432
           +  S  +   +F AKSDYV  PI   GLEGLW KL+EE+    I+ PYGG M++I E++T
Sbjct: 372 DGKS--LFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETET 431

Query: 433 PFPHREGRVFGIQYLATWDNANETE-KHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG 492
           PFPHR G +F IQ+L  W +   +E KH+ W+RE+Y+YME YVSKSPR+AY+NYRDLDLG
Sbjct: 432 PFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLG 491

Query: 493 TNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 530
            N G+ +   +A+ WG +YF  NFERLV +K K DP NFF +EQSIP
Sbjct: 492 MN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Chy12G210100 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 538.1 bits (1385), Expect = 8.3e-153
Identity = 263/527 (49.91%), Postives = 367/527 (69.64%), Query Frame = 0

Query: 9   LFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLLRLSI 68
           + +LF V  IW S SSS +  + F +C  S S  KH  P+S  +F + + S+SS+L+ +I
Sbjct: 6   ILVLFFVVFIWQS-SSSSANSETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANI 65

Query: 69  RNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYIS-SQAP 128
           RNLRF  T+ PKP L++   HESHVQAAI C +   LQ+++RSGGHDY+GLSY++ S  P
Sbjct: 66  RNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKP 125

Query: 129 FIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHIS 188
           F ++D+ NLRS+ +D+ ++TA V+TGA LGE+YY I +KS    +PAG CPTVGVGGHIS
Sbjct: 126 FFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHIS 185

Query: 189 GGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKL 248
           GGG+G + RKYGL  DN IDA+++D NG+I+DR  MGEDL+WAI GGGG S+GV+L++K+
Sbjct: 186 GGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKI 245

Query: 249 KLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVT-NFPSKKTIR 308
            LV VP  VTVF + RTLEQ AT +  +WQ ++ KL  ++F+     VV     S+KT+R
Sbjct: 246 NLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVR 305

Query: 309 LSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLEVLMERS 368
            +F ++FLG    L+ I+  RF ELGL R+DC E SWIQSVLF+ +  + +   +L++R+
Sbjct: 306 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN 365

Query: 369 SPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQTPFP 428
            P     +   KSDYV  PIS  GLE +W K++E E   + F PYGG+M +IS + TPFP
Sbjct: 366 QPV---NYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFP 425

Query: 429 HREGRVFGIQYLATWDNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTN-- 488
           +R G ++ IQY A W +   T++++   R++Y +M  +VSK+PR ++ NYRD+DLG N  
Sbjct: 426 YRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSH 485

Query: 489 YGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLL 532
            G+ +SY E K +G KYF+ NFERLV++KT+VD  NFF NEQSIP+L
Sbjct: 486 NGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Chy12G210100 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 532.7 bits (1371), Expect = 3.5e-151
Identity = 276/537 (51.40%), Postives = 371/537 (69.09%), Query Frame = 0

Query: 7   IHLFLLFCVSLIWL-SHSSSDSLQDNFLECFNSTSYSKHSIPVSEV------VFTNESAS 66
           + LFL+F V   W+ S SSS   +D FL C ++ S S    P S +      V+T+ S S
Sbjct: 9   LSLFLVFLVK--WVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQS 68

Query: 67  FSSLLRLSIRNLRFLAT--TLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYE 126
             S      +N RFL    T  KP+L+VTP  ++ +Q +++C+R+ G++VR +SGGHDYE
Sbjct: 69  LIS------QNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYE 128

Query: 127 GLSYISSQAPFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSC 186
           GLSY+S  +PFII+DL+N+RSI I++  ETA V  GA +GELYY+IAK S IHGFPAG+C
Sbjct: 129 GLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTC 188

Query: 187 PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMD-RSSMGEDLFWAIRGGGG 246
           P+VGVGGH SGGGFG + RK+GLAADNV+DA+ +D NGRI + R  MGEDLFWAIRGGG 
Sbjct: 189 PSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGA 248

Query: 247 ASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVV 306
           ASFGV+LSWK+KLV VP  VT F     L Q  T +  +WQ I+ +LD ++F+ V   + 
Sbjct: 249 ASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSI- 308

Query: 307 TNFPSKKTIRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSID 366
               S  +++ +F + +LG I+KLIP+M  +F ELGLT  DC EM+WI S+++F ++   
Sbjct: 309 ----SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF-NWKKG 368

Query: 367 TPLEVLMERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMS 426
            PLE L++R   + +D +F AKSD+V +PI E GLEG+W++  E E   +I  P GGKM 
Sbjct: 369 QPLETLLDRGQ-RYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLGGKMY 428

Query: 427 QISESQTPFPHREGRVFGIQYLATW--DNANETEKHLSWIREVYAYMESYVSKSPRAAYL 486
           +I E++TPFPHR G ++ IQY+  W   +    EKH++W+R +Y YM  YVS SPR AYL
Sbjct: 429 EIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYL 488

Query: 487 NYRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLL 532
           NYRDLDLG N G NTS+E+AK+WG +YF  NF+RL  VK K+DP+NFF NEQS+P L
Sbjct: 489 NYRDLDLGMNRGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPL 530

BLAST of Chy12G210100 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 530.4 bits (1365), Expect = 1.7e-150
Identity = 268/531 (50.47%), Postives = 367/531 (69.11%), Query Frame = 0

Query: 7   IHLFLLFCVSLIWLSHSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLLRL 66
           I +F L+  S   ++ +SS SLQD+F++C    +  + ++  +       ++ F+ +L  
Sbjct: 13  ISVFALY-FSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLES 72

Query: 67  SIRNLRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYISS-Q 126
           + +N R+L  T+PKP  +  P HESHVQA+++C+++  +  RVRSGGHDYEG+SY+S  +
Sbjct: 73  TAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIE 132

Query: 127 APFIIIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGH 186
            PF++IDL  LR I +DIK  +A VE GA +GELYYRIA+KS  HGFPAG  P++G+GGH
Sbjct: 133 KPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGH 192

Query: 187 ISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSW 246
           I+GG +G+L RKYGLAADNV+DAKI+D NG+++DR+SMGEDLFWAIRGG G SFG+ILSW
Sbjct: 193 ITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSW 252

Query: 247 KLKLVSVPSIVTVFNVQRTLEQGAT-HLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKKT 306
           K+KLV VP  +TVF V +T EQ  +  +  KWQ I+  L  ++FL V   V  N  + KT
Sbjct: 253 KIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN-KANKT 312

Query: 307 IRLSFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSID--TPLEVL 366
           + +++   FLG    L+ +MK  F ELGLT+ DCIEMSWI S+++ + F  +   P+E+L
Sbjct: 313 VTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEIL 372

Query: 367 MERSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQ 426
           ++  SP I   +F  KSD+   PI   GLEG++ KL+EE+ + +I+TPYGGKM +I ES+
Sbjct: 373 LQAKSP-IGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESE 432

Query: 427 TPFPHREGRVFGIQYLATW-DNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDL 486
            PFPHR G  F IQY  +W D+     +   WIRE+Y YM  YVS +PR AY+NYRDLDL
Sbjct: 433 IPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDL 492

Query: 487 GTNYGRNTS-YEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLL 532
           G N   + S + EAK+WG  YF DNF RLVR+K+KVDP NFF +EQSIP L
Sbjct: 493 GQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of Chy12G210100 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 529.6 bits (1363), Expect = 3.0e-150
Identity = 261/523 (49.90%), Postives = 359/523 (68.64%), Query Frame = 0

Query: 12  LFCVSLIWLS-HSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLLRLSIRN 71
           LF V+L+ +S   SS +LQ +F++C    S     I  S       +  F   L  + +N
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 72  LRFLATTLPKPLLLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYIS-SQAPFI 131
           LR+L  + PKP+ +  P +E+HVQAA+VCA++  L +R+RSGGHDYEGLS+++  + PF+
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 131

Query: 132 IIDLINLRSITIDIKTETASVETGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGG 191
           I+DL  LR + +D+ + +A    GA +GE+YYRI +KS  HGFPAG C ++G+GGH+ GG
Sbjct: 132 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 191

Query: 192 GFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKL 251
            +G++ RK+GL ADNV+DA+I+D NG+I+DR++MGED+FWAIRGGGG SFGVIL+WK+KL
Sbjct: 192 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 251

Query: 252 VSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVV--TNFPSKKTIRL 311
           V VP+ VTVF V +TLEQ  T +  KW+ I+ KLD D+F+ V       T  P  +TI +
Sbjct: 252 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISM 311

Query: 312 SFTSLFLGPIEKLIPIMKTRFSELGLTRNDCIEMSWIQSVLFFADFSIDTPLEVLMERSS 371
           S+ + FLG   +L+ +M+  F ELGLT+ DC EMSWI+SV++ A F      E L+   S
Sbjct: 312 SYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKS 371

Query: 372 PQISDAFFTAKSDYVTSPISENGLEGLWSKLVEEEKSELIFTPYGGKMSQISESQTPFPH 431
             +    F AKSD+V  PI   GLEGLW + +EE+    I+ PYGG MS+ISES+ PFPH
Sbjct: 372 --LFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPH 431

Query: 432 REGRVFGIQYLATW-DNANETEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYG 491
           R G +F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLGTN G
Sbjct: 432 RNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEG 491

Query: 492 RNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 530
                 +A+ WG KY+  NFERLV++K + DP NFF +EQS+P
Sbjct: 492 ET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528