Chy12G209680 (gene) Cucumber (hystrix) v1

Overview
NameChy12G209680
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein kinase PINOID
LocationchrH12: 4176145 .. 4178113 (-)
RNA-Seq ExpressionChy12G209680
SyntenyChy12G209680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTACAACACCTTCCCCCAGAATCAGACGCTCTTAATTCCAATTCCAGCCAAAATTCTATGAGCAGCGAGAGTTGTAGTAGCTTCAGTCGTCTTTCATTTGATGCCGCCATTGATTTACCCACTTCCCGATCTTCACCCGCCAGTCTCCATTTAAAGCCACATCGTTCATCTGATTTCGCTTACTCCGCTATTCGTCGTCGCAAATCTAAACTCACGTTTCGTGATTTTCGACTTCTCCGTCGGATTGGTGCTGGCGACATTGGTACTGTTTATTTGTGTCAGTTGAGGAACTTGAGTAAAGGGTGTTGTTACGAAGACGAAGATGATGAAGATGACGACGTCGACGAGGAGGATGATGATGATGATGATGTTTCGTGTTTGTATGCGATGAAAGTTGTGGATAAAGACGCGTTGGAATTGAAGAAGAAAGTTCAGAGAGCGGAAATGGAAAGGAAAATATTGAAGATGCTTGATCATCCGTTTTTGCCTACACTTTACGCTGAGTTTGAAGCTTCTCATTTCTCTTGTATTGTTATGGAGTTTTGTTCTGGTGGGGATTTGCATTCTCTCCGCCATAAACAACCACGGAAACGCTTCTCTCTCACCTCCGCTAGGTACTGTCATACAGATCATCATCATCATCCTTCGCCCTTTTCTTTTCTTCACTGAGATATTATGTAGATTCTTGGAAAGCTCTTTTGGAACGATTTTAAAAATATTAACGTTTAAAAATATAAAATTGAATTCAGAGTTTTCAGTATGGTTTTTGTTTGGTTGTGGTGTGAAAACCTGAGATGTTACAATCTTGTCTGCTTTAGCTTTCTGTGATGTCTCCTTCAATGGAAATTATTCATTTTCTTTCTCTGGGAAAAATTCATTTTTAATATTAATTAATGCATCTCTGCTCTGCTGCCGAGATTGGGTTCGATGAATATCTTCTGACTTTTATTTTAAAAAATTTCCATTTTAATGAAGTGGGGTTCGTTTATCCTCTGCTTTTCTCTTCCTCTCTGCATTTTCTTCGTCAGGAATTTATCACCGTTCTTGTGCAACAACTTACTTCACATAATTATTACTTTTTTTCTCTTGTGAGTATGATATTACATTTTTTTCCTGTAGAATTAATTAACCTGAATTGATTTTGTGTTTGATTTTCAGGTTTTATGCAGCGGAAGTTCTTGTTGCTTTAGAGTACCTTCATATGCTTGGAATCATTTACAGAGACCTTAAACCGGAGAATGTTCTCGTCCGATCAGACGGTCACATTATGTTGTCGGATTTTGATTTATCTCTCTGTTCCGACGCCACCCCTGCCGTTGAATCACCCAACTCTTCCCCGAACACGGAATTTCCCGAATCAACGGCGTACCCCAAAACCAACGCTCCCGTTCCCGCCGCCTCCGCAGCTCCTTTTTCCTGCCTGTACAACCGGCTTTTCCGGTCGAGGAAGGTCCAAACTCTGTCTCCCAACTGGCTCTTCGTCGCTGAGCCCGTTTCCGCTAGGTCCTGCTCCTTCGTTGGTACTCACGAGTACGTCTCGCCGGAAGTTGCCGCCGGCGGGTCTCACGGTAACGCCGTGGACTGGTGGGCCTTTGGAATTTTTCTCTACGAGTTGATCTACGGCCGGACGCCGTTTGCGGCTTCGTCGAACGAGACGACGTTGAGAAGCATTATAAAGAAACCGTTGACGTTTCCCACAGCCGTGCCGTCTGGCGCGCTTGAACACCACGCGCGGGACCTCATATCCGGTTTACTGAATAAGGACCCGACCCGGAGACTGGGGTCGAAACGCGGGTCCGCCGACATCAAGAAGCATCCATTCTTCAAAGGCCTCAACTTTGCGTTAATTCGATCCCTCACTCCTCCGGAAATCCCCGGGGTGCGACGGCGTAGGGCGCCGCCAGCTCCGGTGGCGAAAAATAAGGATCACAAGTCCGGTCAGTCGACCGGGTTCGATTATTTCTGA

mRNA sequence

ATGTTACAACACCTTCCCCCAGAATCAGACGCTCTTAATTCCAATTCCAGCCAAAATTCTATGAGCAGCGAGAGTTGTAGTAGCTTCAGTCGTCTTTCATTTGATGCCGCCATTGATTTACCCACTTCCCGATCTTCACCCGCCAGTCTCCATTTAAAGCCACATCGTTCATCTGATTTCGCTTACTCCGCTATTCGTCGTCGCAAATCTAAACTCACGTTTCGTGATTTTCGACTTCTCCGTCGGATTGGTGCTGGCGACATTGGTACTGTTTATTTGTGTCAGTTGAGGAACTTGAGTAAAGGGTGTTGTTACGAAGACGAAGATGATGAAGATGACGACGTCGACGAGGAGGATGATGATGATGATGATGTTTCGTGTTTGTATGCGATGAAAGTTGTGGATAAAGACGCGTTGGAATTGAAGAAGAAAGTTCAGAGAGCGGAAATGGAAAGGAAAATATTGAAGATGCTTGATCATCCGTTTTTGCCTACACTTTACGCTGAGTTTGAAGCTTCTCATTTCTCTTGTATTGTTATGGAGTTTTGTTCTGGTGGGGATTTGCATTCTCTCCGCCATAAACAACCACGGAAACGCTTCTCTCTCACCTCCGCTAGGTTTTATGCAGCGGAAGTTCTTGTTGCTTTAGAGTACCTTCATATGCTTGGAATCATTTACAGAGACCTTAAACCGGAGAATGTTCTCGTCCGATCAGACGGTCACATTATGTTGTCGGATTTTGATTTATCTCTCTGTTCCGACGCCACCCCTGCCGTTGAATCACCCAACTCTTCCCCGAACACGGAATTTCCCGAATCAACGGCGTACCCCAAAACCAACGCTCCCGTTCCCGCCGCCTCCGCAGCTCCTTTTTCCTGCCTGTACAACCGGCTTTTCCGGTCGAGGAAGGTCCAAACTCTGTCTCCCAACTGGCTCTTCGTCGCTGAGCCCGTTTCCGCTAGGTCCTGCTCCTTCGTTGGTACTCACGAGTACGTCTCGCCGGAAGTTGCCGCCGGCGGGTCTCACGGTAACGCCGTGGACTGGTGGGCCTTTGGAATTTTTCTCTACGAGTTGATCTACGGCCGGACGCCGTTTGCGGCTTCGTCGAACGAGACGACGTTGAGAAGCATTATAAAGAAACCGTTGACGTTTCCCACAGCCGTGCCGTCTGGCGCGCTTGAACACCACGCGCGGGACCTCATATCCGGTTTACTGAATAAGGACCCGACCCGGAGACTGGGGTCGAAACGCGGGTCCGCCGACATCAAGAAGCATCCATTCTTCAAAGGCCTCAACTTTGCGTTAATTCGATCCCTCACTCCTCCGGAAATCCCCGGGGTGCGACGGCGTAGGGCGCCGCCAGCTCCGGTGGCGAAAAATAAGGATCACAAGTCCGGTCAGTCGACCGGGTTCGATTATTTCTGA

Coding sequence (CDS)

ATGTTACAACACCTTCCCCCAGAATCAGACGCTCTTAATTCCAATTCCAGCCAAAATTCTATGAGCAGCGAGAGTTGTAGTAGCTTCAGTCGTCTTTCATTTGATGCCGCCATTGATTTACCCACTTCCCGATCTTCACCCGCCAGTCTCCATTTAAAGCCACATCGTTCATCTGATTTCGCTTACTCCGCTATTCGTCGTCGCAAATCTAAACTCACGTTTCGTGATTTTCGACTTCTCCGTCGGATTGGTGCTGGCGACATTGGTACTGTTTATTTGTGTCAGTTGAGGAACTTGAGTAAAGGGTGTTGTTACGAAGACGAAGATGATGAAGATGACGACGTCGACGAGGAGGATGATGATGATGATGATGTTTCGTGTTTGTATGCGATGAAAGTTGTGGATAAAGACGCGTTGGAATTGAAGAAGAAAGTTCAGAGAGCGGAAATGGAAAGGAAAATATTGAAGATGCTTGATCATCCGTTTTTGCCTACACTTTACGCTGAGTTTGAAGCTTCTCATTTCTCTTGTATTGTTATGGAGTTTTGTTCTGGTGGGGATTTGCATTCTCTCCGCCATAAACAACCACGGAAACGCTTCTCTCTCACCTCCGCTAGGTTTTATGCAGCGGAAGTTCTTGTTGCTTTAGAGTACCTTCATATGCTTGGAATCATTTACAGAGACCTTAAACCGGAGAATGTTCTCGTCCGATCAGACGGTCACATTATGTTGTCGGATTTTGATTTATCTCTCTGTTCCGACGCCACCCCTGCCGTTGAATCACCCAACTCTTCCCCGAACACGGAATTTCCCGAATCAACGGCGTACCCCAAAACCAACGCTCCCGTTCCCGCCGCCTCCGCAGCTCCTTTTTCCTGCCTGTACAACCGGCTTTTCCGGTCGAGGAAGGTCCAAACTCTGTCTCCCAACTGGCTCTTCGTCGCTGAGCCCGTTTCCGCTAGGTCCTGCTCCTTCGTTGGTACTCACGAGTACGTCTCGCCGGAAGTTGCCGCCGGCGGGTCTCACGGTAACGCCGTGGACTGGTGGGCCTTTGGAATTTTTCTCTACGAGTTGATCTACGGCCGGACGCCGTTTGCGGCTTCGTCGAACGAGACGACGTTGAGAAGCATTATAAAGAAACCGTTGACGTTTCCCACAGCCGTGCCGTCTGGCGCGCTTGAACACCACGCGCGGGACCTCATATCCGGTTTACTGAATAAGGACCCGACCCGGAGACTGGGGTCGAAACGCGGGTCCGCCGACATCAAGAAGCATCCATTCTTCAAAGGCCTCAACTTTGCGTTAATTCGATCCCTCACTCCTCCGGAAATCCCCGGGGTGCGACGGCGTAGGGCGCCGCCAGCTCCGGTGGCGAAAAATAAGGATCACAAGTCCGGTCAGTCGACCGGGTTCGATTATTTCTGA

Protein sequence

MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDFAYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFRSRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF*
Homology
BLAST of Chy12G209680 vs. ExPASy Swiss-Prot
Match: O64682 (Protein kinase PINOID OS=Arabidopsis thaliana OX=3702 GN=PID PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.3e-156
Identity = 302/471 (64.12%), Postives = 354/471 (75.16%), Query Frame = 0

Query: 8   ESD---ALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDFAYSA 67
           ESD   +L + +S  S  +ESCSSFSRLSFDA     T     + L LKPHRSSDFAY+ 
Sbjct: 4   ESDGEMSLGTTNSPISSGTESCSSFSRLSFDAPPS--TIPEEESFLSLKPHRSSDFAYAE 63

Query: 68  IRRRKSK-LTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDDD 127
           IRRRK + LTFRDFRL+RRIGAGDIGTVYLC+L                       D+++
Sbjct: 64  IRRRKKQGLTFRDFRLMRRIGAGDIGTVYLCRLAG---------------------DEEE 123

Query: 128 DVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFC 187
             S  +AMKVVDK+AL LKKK+ RAEME+ ILKMLDHPFLPTLYAEFEASHFSCIVME+C
Sbjct: 124 SRSSYFAMKVVDKEALALKKKMHRAEMEKTILKMLDHPFLPTLYAEFEASHFSCIVMEYC 183

Query: 188 SGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIM 247
           SGGDLHSLRH+QP +RFSL+SARFYAAEVLVALEYLHMLGIIYRDLKPEN+LVRSDGHIM
Sbjct: 184 SGGDLHSLRHRQPHRRFSLSSARFYAAEVLVALEYLHMLGIIYRDLKPENILVRSDGHIM 243

Query: 248 LSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFRSRK 307
           LSDFDLSLCSD+  AVES +SSP  +        +  +P      A    L+ R+ RS+K
Sbjct: 244 LSDFDLSLCSDSIAAVESSSSSPENQ--------QLRSPRRFTRLAR---LFQRVLRSKK 303

Query: 308 VQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIYGRT 367
           VQTL P  LFVAEPV+ARS SFVGTHEYV+PEVA+GGSHGNAVDWWAFG+FLYE+IYG+T
Sbjct: 304 VQTLEPTRLFVAEPVTARSGSFVGTHEYVAPEVASGGSHGNAVDWWAFGVFLYEMIYGKT 363

Query: 368 PFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSADIK 427
           PF A +N+  LR+I+K+ L+FPT  P+   E HAR+LISGLLNKDPT+RLGS+RG+A++K
Sbjct: 364 PFVAPTNDVILRNIVKRQLSFPTDSPATMFELHARNLISGLLNKDPTKRLGSRRGAAEVK 423

Query: 428 KHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 475
            HPFFKGLNFALIR+LTPPEIP    ++  P   A      S +   FDYF
Sbjct: 424 VHPFFKGLNFALIRTLTPPEIPSSVVKK--PMKSATFSGRSSNKPAAFDYF 438

BLAST of Chy12G209680 vs. ExPASy Swiss-Prot
Match: Q2QM77 (Protein kinase PINOID OS=Oryza sativa subsp. japonica OX=39947 GN=PID PE=2 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.7e-122
Identity = 246/485 (50.72%), Postives = 325/485 (67.01%), Query Frame = 0

Query: 2   LQHLPPESDALNSNSSQNSMSSES-----CSSFSRLSFD----AAIDLPTSRSSP--ASL 61
           ++    ++D    NSS +S +S +     CSS SRLSFD    AA+    +  SP  AS+
Sbjct: 21  MERYSSDADTTAPNSSLSSAASSTGSLARCSSLSRLSFDCSPSAAVAAAATSCSPPRASV 80

Query: 62  HLKPHRSSDFAYSAIRRRK----SKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYE 121
            L+PHRS D A++AIR       + L  RDF+L+RRIG GDIGTVYLC+LR+        
Sbjct: 81  LLRPHRSGDVAWAAIRAASTTSAAPLGPRDFKLVRRIGGGDIGTVYLCRLRS-------- 140

Query: 122 DEDDEDDDVDEEDDDDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTL 181
                          + +  C+YAMKVVD+ A+  K+K+ RA  E++IL+ LDHPFLPTL
Sbjct: 141 -------------SPERESPCMYAMKVVDRRAVARKQKLGRAAAEKRILRQLDHPFLPTL 200

Query: 182 YAEFEAS-HFSCIVMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGII 241
           +A+F+A+ HFSC VMEFC GGDLHSLRH+ P +RF L SARFYAAEVL+A+EYLHM+GI+
Sbjct: 201 FADFDATPHFSCAVMEFCPGGDLHSLRHRMPSRRFPLPSARFYAAEVLLAIEYLHMMGIV 260

Query: 242 YRDLKPENVLVRSDGHIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPA 301
           YRDLKPENVL+R+DGHIML+DFDLSL S  +P+++    + +     ++ +P        
Sbjct: 261 YRDLKPENVLIRADGHIMLTDFDLSLQSTTSPSLDGDTDTDDEASGGASCFPD------- 320

Query: 302 ASAAPFSCLYNRLFRSRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNA 361
                    +   F+ R+    +P   FVAEPV ARSCSFVGTHEYV+PEVA+GG+HG A
Sbjct: 321 ---------HLLRFKRRRNAVAAPRPRFVAEPVDARSCSFVGTHEYVAPEVASGGAHGAA 380

Query: 362 VDWWAFGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPS-GALEHHARDLISGL 421
           VDWWA+G+FLYELIYGRTPFA ++NE TLR+I+++PL FP+   S G  +  ARDLI+ L
Sbjct: 381 VDWWAYGVFLYELIYGRTPFAGATNEATLRNIVRRPLAFPSGSGSCGPADADARDLIARL 440

Query: 422 LNKDPTRRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHK 470
           L KDP  RLGS+RG+AD+K HPFFK LN AL+RS  PP +PG     A  AP+ +++  K
Sbjct: 441 LAKDPAARLGSRRGAADVKSHPFFKSLNLALLRSSRPPVVPGA---GAGAAPLHRSQSCK 465

BLAST of Chy12G209680 vs. ExPASy Swiss-Prot
Match: Q94E49 (Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.3e-106
Identity = 232/498 (46.59%), Postives = 303/498 (60.84%), Query Frame = 0

Query: 8   ESDALNSNSSQNSMSSE--------SCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSD 67
           ESD  +S SS  +  S         S SS S  SF    D P S     S   KPH+++ 
Sbjct: 7   ESDYDSSRSSLTAPDSRRSWISDIGSSSSVSARSFGG--DTPAS-----SCRYKPHKANQ 66

Query: 68  FAYSAIRRRKS---KLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVD 127
             + AIRR ++   ++    FRL+RR+G+GD+G VYLC+LR               +   
Sbjct: 67  AEWEAIRRLRAGAGRVGLEHFRLVRRLGSGDLGNVYLCRLR---------------EPWS 126

Query: 128 EEDDDDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFS 187
                     CLYAMKVVDKDAL  +KK++RAE+ER IL+ LDHPFLPTLYA+FEASH++
Sbjct: 127 SSSMTTTAGGCLYAMKVVDKDALAFRKKLRRAEVERDILRTLDHPFLPTLYADFEASHYA 186

Query: 188 CIVMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLV 247
           C+VMEFC GGDLH  R +QP +RF+++S RFY AE ++ALEYLHM+G++YRDLKPENVLV
Sbjct: 187 CLVMEFCPGGDLHVARQRQPGRRFTVSSTRFYVAETVLALEYLHMMGVVYRDLKPENVLV 246

Query: 248 RSDGHIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAP-----F 307
           R DGHIMLSDFDLSL  D  P +  P  S       + A  K   P P++   P      
Sbjct: 247 RGDGHIMLSDFDLSLKCDVVPKLLRPARS-------AAAGGKPPLPPPSSCVPPTIQPVL 306

Query: 308 SCLYNRLFR-------------------------SRKVQTLSPNWLFVAEPVSARSCSFV 367
           SC++  + +                           + +T  P  + V EPV+ARS SFV
Sbjct: 307 SCIFRGVHKCHHAKECAGGGAAAGNNGDGDGNDEEAETETAEPE-VVVVEPVAARSKSFV 366

Query: 368 GTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPT 427
           GTHEY++PEV +G  HG+AVDWW  G+F+YE++YGRTPF   SNE TL +IIK+P+TFP 
Sbjct: 367 GTHEYLAPEVISGQGHGSAVDWWTLGVFMYEMLYGRTPFKGESNEKTLINIIKQPVTFPR 426

Query: 428 ---AVPSGALEH--HARDLISGLLNKDPTRRLGSKRGSADIKKHPFFKGLNFALIRSLTP 460
              A  +G  E    A+DL+  LL K+P +RLGS  GSA++K+HPFFKG+N+AL+RS+ P
Sbjct: 427 LAGAAAAGEWEEMKTAQDLMLQLLAKNPKKRLGSTMGSAEVKRHPFFKGVNWALVRSVRP 468

BLAST of Chy12G209680 vs. ExPASy Swiss-Prot
Match: Q9FG74 (Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.6e-104
Identity = 228/497 (45.88%), Postives = 292/497 (58.75%), Query Frame = 0

Query: 2   LQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDFA 61
           L    P SD    NS  N       SS  R S  + I   ++ SS +S   KPH+++D  
Sbjct: 31  LSFADPSSDGKTGNSKINEQGESGKSSTCRPSTSSDISDESTCSSFSSSINKPHKANDVR 90

Query: 62  YSAIRRRKSK---LTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEE 121
           + AI+  ++K   L    FRLL+R+G GDIGTV+L +L                      
Sbjct: 91  WEAIQAVRTKHGGLGLNHFRLLKRLGCGDIGTVHLAEL---------------------- 150

Query: 122 DDDDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCI 181
               +   C +AMKV+DK AL  +KK+ RA+ ER+IL+ LDHPFLPTLY+ FE   FSC+
Sbjct: 151 ----NGTRCYFAMKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCL 210

Query: 182 VMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRS 241
           VMEFC GGDLH+LR +QP KRF+  +A+FY AEVL+A+EYLHMLGIIYRDLKPENVLVR 
Sbjct: 211 VMEFCPGGDLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRD 270

Query: 242 DGHIMLSDFDLSL-CSDATPAVESPNSSPNTEFPESTAYPKTNAPV---PAASAAPFSCL 301
           DGH+MLSDFDLSL C+ +   V S N         S +  +  A +      S AP SC 
Sbjct: 271 DGHVMLSDFDLSLRCTVSLSIVRSANVGSEGLSKNSVSCSQQPACIQQPSCISMAPTSCF 330

Query: 302 YNRLFRSRKVQTLSPNW-----------LFVAEPVSARSCSFVGTHEYVSPEVAAGGSHG 361
             R F S+  +   P               VAEP  ARS SFVGTHEY++PE+  G  HG
Sbjct: 331 GPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEGHG 390

Query: 362 NAVDWWAFGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISG 421
           +AVDWW FGIFLYEL++G+TPF  S N  TL +++ +PL FP    S  +   ARDLI  
Sbjct: 391 SAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFP---ESPVVSFAARDLIRS 450

Query: 422 LLNKDPTRRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEIPGVRRRRA----PPAPVAK 475
           LL K+P  RL  KRG+ +IK+HPFF+G+N+AL+R  +PPEIP      A    P  P A 
Sbjct: 451 LLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEIPKPVDLEALNPTPTVPAAA 498

BLAST of Chy12G209680 vs. ExPASy Swiss-Prot
Match: Q9SUA3 (Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1 PE=1 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 6.6e-103
Identity = 223/488 (45.70%), Postives = 295/488 (60.45%), Query Frame = 0

Query: 7   PESDALNSNSSQNSMSSESCSSFSRLSFDAAIDL-PTSRSSPASLHLKPHRSSDFAYSAI 66
           P SD+      + +  S+     +      + D+   S  S  S + KPH+++D  + AI
Sbjct: 49  PSSDSKTGGEVKFNEKSDQSGKSNTCRPSTSSDISDESTCSSFSGNNKPHKANDVRWEAI 108

Query: 67  RRRKSK---LTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDD 126
           +  ++K   L    FRLL+R+G GDIGTV+L +L                          
Sbjct: 109 QAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLAELHG------------------------ 168

Query: 127 DDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEF 186
               C +AMKV+DK AL  +KK+ RA+ ER+IL+ LDHPFLPTLY+ FE   FSC+VMEF
Sbjct: 169 --TRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEF 228

Query: 187 CSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHI 246
           C GGDLH+LR +QP KRFS  +A+FY AEVL+A+EYLHMLGIIYRDLKPENVLVR DGH+
Sbjct: 229 CPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHV 288

Query: 247 MLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPV--PAASAAPFSCLYNRLFR 306
           MLSDFDLSL    +P V   ++   +E  +++ Y    A +  P+  +AP +C   R F 
Sbjct: 289 MLSDFDLSLRCTVSPTVVR-STVLASEGQKNSGYCAQPACIQQPSCISAPTTCFSPRYFS 348

Query: 307 SRKVQ----------TLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWA 366
           S+  +           +SP    VAEP SARS SFVGTHEY++PE+  G  HG+AVDWW 
Sbjct: 349 SKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVDWWT 408

Query: 367 FGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPT 426
           FGIFLYEL++G+TPF  S N  TL +++ +PL FP    S  +   ARDLI  LL K+P 
Sbjct: 409 FGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFP---ESPVVSFAARDLIRSLLVKEPQ 468

Query: 427 RRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEIP-GVRRRRAPPAPVAKNKDH-KSGQS 475
            RL  KRG+ ++K+HPFF+G+N+AL+R  +PPEIP  V    AP  P A      KS QS
Sbjct: 469 HRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIPKPVDYESAPATPAAATSTSVKSDQS 506

BLAST of Chy12G209680 vs. ExPASy TrEMBL
Match: A0A0A0LVF0 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537400 PE=4 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.5e-262
Identity = 464/474 (97.89%), Postives = 466/474 (98.31%), Query Frame = 0

Query: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60
           MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF
Sbjct: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60

Query: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDD 120
           AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVD+EDD
Sbjct: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDDEDD 120

Query: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180
           DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM
Sbjct: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180

Query: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240
           EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG
Sbjct: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240

Query: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFR 300
           HIMLSDFDLSLCSDATPAVESPNSSPNT F ESTAYPKTN  V AASAAPFSCLYNRLFR
Sbjct: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTAFHESTAYPKTNVAVSAASAAPFSCLYNRLFR 300

Query: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360
           SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY
Sbjct: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360

Query: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420
           GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA
Sbjct: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420

Query: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 475
           DIKKHPFFKGLNFALIRSLTPPEIPGVRRR+A PAPVAK KD KSGQSTGFDYF
Sbjct: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRKATPAPVAKTKDQKSGQSTGFDYF 474

BLAST of Chy12G209680 vs. ExPASy TrEMBL
Match: A0A5A7UE24 (Protein kinase PINOID OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G001070 PE=4 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 2.8e-258
Identity = 455/474 (95.99%), Postives = 463/474 (97.68%), Query Frame = 0

Query: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60
           MLQHLP ESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF
Sbjct: 1   MLQHLPSESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60

Query: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDD 120
           AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDD  +++D
Sbjct: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDDIDDED 120

Query: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180
           DDDDVSCLYAMKVVDK+ALELKKKV RAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM
Sbjct: 121 DDDDVSCLYAMKVVDKEALELKKKVHRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180

Query: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240
           EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG
Sbjct: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240

Query: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFR 300
           HIMLSDFDLSLCSDATPAVESPNSSPNT FPE+TAYPKTN  V A SAAPFSCLYNRLFR
Sbjct: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTAFPETTAYPKTNPAVSATSAAPFSCLYNRLFR 300

Query: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360
           SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY
Sbjct: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360

Query: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420
           GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA
Sbjct: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420

Query: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 475
           DIKKHPFFKGLNFALIRSLTPPE+PGVRRR+A PAPVAK KDHKSGQ+TGFDYF
Sbjct: 421 DIKKHPFFKGLNFALIRSLTPPEVPGVRRRKATPAPVAKTKDHKSGQATGFDYF 474

BLAST of Chy12G209680 vs. ExPASy TrEMBL
Match: A0A1S3BC27 (protein kinase PINOID OS=Cucumis melo OX=3656 GN=LOC103488038 PE=4 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 2.8e-258
Identity = 455/474 (95.99%), Postives = 463/474 (97.68%), Query Frame = 0

Query: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60
           MLQHLP ESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF
Sbjct: 1   MLQHLPSESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60

Query: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDD 120
           AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDD  +++D
Sbjct: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDDIDDED 120

Query: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180
           DDDDVSCLYAMKVVDK+ALELKKKV RAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM
Sbjct: 121 DDDDVSCLYAMKVVDKEALELKKKVHRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180

Query: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240
           EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG
Sbjct: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240

Query: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFR 300
           HIMLSDFDLSLCSDATPAVESPNSSPNT FPE+TAYPKTN  V A SAAPFSCLYNRLFR
Sbjct: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTAFPETTAYPKTNPAVSATSAAPFSCLYNRLFR 300

Query: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360
           SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY
Sbjct: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360

Query: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420
           GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA
Sbjct: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420

Query: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 475
           DIKKHPFFKGLNFALIRSLTPPE+PGVRRR+A PAPVAK KDHKSGQ+TGFDYF
Sbjct: 421 DIKKHPFFKGLNFALIRSLTPPEVPGVRRRKATPAPVAKTKDHKSGQATGFDYF 474

BLAST of Chy12G209680 vs. ExPASy TrEMBL
Match: A0A5D3BDZ9 (Protein kinase PINOID OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00440 PE=4 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 6.1e-229
Identity = 416/474 (87.76%), Postives = 421/474 (88.82%), Query Frame = 0

Query: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60
           MLQHLP ESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF
Sbjct: 1   MLQHLPSESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60

Query: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDD 120
           AYSAIRRRKSKLTFRDFRLLRRIGAGDI                                
Sbjct: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDI-------------------------------- 120

Query: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180
                       VVDK+ALELKKKV RAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM
Sbjct: 121 ------------VVDKEALELKKKVHRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180

Query: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240
           EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG
Sbjct: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240

Query: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFR 300
           HIMLSDFDLSLCSDATPAVESPNSSPNT FPE+TAYPKTN  V A SAAPFSCLYNRLFR
Sbjct: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTAFPETTAYPKTNPAVSATSAAPFSCLYNRLFR 300

Query: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360
           SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY
Sbjct: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360

Query: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420
           GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA
Sbjct: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420

Query: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 475
           DIKKHPFFKGLNFALIRSLTPPE+PGVRRR+A PAPVAK KDHKSGQ+TGFDYF
Sbjct: 421 DIKKHPFFKGLNFALIRSLTPPEVPGVRRRKATPAPVAKTKDHKSGQATGFDYF 430

BLAST of Chy12G209680 vs. ExPASy TrEMBL
Match: A0A6J1GR98 (protein kinase PINOID-like OS=Cucurbita moschata OX=3662 GN=LOC111456752 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.5e-222
Identity = 406/467 (86.94%), Postives = 424/467 (90.79%), Query Frame = 0

Query: 11  ALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDFAYSAIRRRKS 70
           A+NSNSSQNSMSSESCSSFSRLSFDAA+D PTSR SP SL LKPHRSSDFAYSAIRRRKS
Sbjct: 18  AVNSNSSQNSMSSESCSSFSRLSFDAAVDFPTSRFSPGSLDLKPHRSSDFAYSAIRRRKS 77

Query: 71  KLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDDDDVSCLYA 130
           KLTFRDFRLLRRIGAGDIGTVYLCQLR L+ G      DDEDDD           SCLYA
Sbjct: 78  KLTFRDFRLLRRIGAGDIGTVYLCQLRKLTDG----GYDDEDDD-----------SCLYA 137

Query: 131 MKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFCSGGDLHS 190
           MKVVDK+ALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFCSGGDLHS
Sbjct: 138 MKVVDKEALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFCSGGDLHS 197

Query: 191 LRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIMLSDFDLS 250
           LRHKQPRKRFSL+SARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIMLSDFDLS
Sbjct: 198 LRHKQPRKRFSLSSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIMLSDFDLS 257

Query: 251 LCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAA---PFSCLYNRLFRSRKVQTL 310
           LCSDATPAVESP+SSPN+ FP++ AYPK+N   P ASAA   PFSC YNRLFRSRKVQTL
Sbjct: 258 LCSDATPAVESPDSSPNSAFPDAMAYPKSNR-TPTASAATTSPFSCFYNRLFRSRKVQTL 317

Query: 311 SPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIYGRTPFAA 370
           +PNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFG+FLYELI+GRTPFAA
Sbjct: 318 TPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGVFLYELIFGRTPFAA 377

Query: 371 SSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSADIKKHPF 430
           SSNETTLR+IIKKPLTFPTA  SG LEHHARDLISGLLNKDPTRRLGSKRGSADIKKHPF
Sbjct: 378 SSNETTLRNIIKKPLTFPTAETSGVLEHHARDLISGLLNKDPTRRLGSKRGSADIKKHPF 437

Query: 431 FKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 475
           FKGLNFALIRSLTPPE+PGVRRR+  PAPVAK+ D K+  S GFDYF
Sbjct: 438 FKGLNFALIRSLTPPEVPGVRRRKPVPAPVAKSMD-KAADSGGFDYF 467

BLAST of Chy12G209680 vs. NCBI nr
Match: XP_004135537.1 (protein kinase PINOID [Cucumis sativus] >KGN65890.1 hypothetical protein Csa_023243 [Cucumis sativus])

HSP 1 Score: 909 bits (2349), Expect = 0.0
Identity = 464/474 (97.89%), Postives = 466/474 (98.31%), Query Frame = 0

Query: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60
           MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF
Sbjct: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60

Query: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDD 120
           AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVD+EDD
Sbjct: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDDEDD 120

Query: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180
           DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM
Sbjct: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180

Query: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240
           EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG
Sbjct: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240

Query: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFR 300
           HIMLSDFDLSLCSDATPAVESPNSSPNT F ESTAYPKTN  V AASAAPFSCLYNRLFR
Sbjct: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTAFHESTAYPKTNVAVSAASAAPFSCLYNRLFR 300

Query: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360
           SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY
Sbjct: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360

Query: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420
           GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA
Sbjct: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420

Query: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 474
           DIKKHPFFKGLNFALIRSLTPPEIPGVRRR+A PAPVAK KD KSGQSTGFDYF
Sbjct: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRKATPAPVAKTKDQKSGQSTGFDYF 474

BLAST of Chy12G209680 vs. NCBI nr
Match: XP_008444803.1 (PREDICTED: protein kinase PINOID [Cucumis melo] >KAA0054042.1 protein kinase PINOID [Cucumis melo var. makuwa])

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 455/474 (95.99%), Postives = 463/474 (97.68%), Query Frame = 0

Query: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60
           MLQHLP ESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF
Sbjct: 1   MLQHLPSESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60

Query: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDD 120
           AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDD  +++D
Sbjct: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDDIDDED 120

Query: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180
           DDDDVSCLYAMKVVDK+ALELKKKV RAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM
Sbjct: 121 DDDDVSCLYAMKVVDKEALELKKKVHRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180

Query: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240
           EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG
Sbjct: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240

Query: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFR 300
           HIMLSDFDLSLCSDATPAVESPNSSPNT FPE+TAYPKTN  V A SAAPFSCLYNRLFR
Sbjct: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTAFPETTAYPKTNPAVSATSAAPFSCLYNRLFR 300

Query: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360
           SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY
Sbjct: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360

Query: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420
           GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA
Sbjct: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420

Query: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 474
           DIKKHPFFKGLNFALIRSLTPPE+PGVRRR+A PAPVAK KDHKSGQ+TGFDYF
Sbjct: 421 DIKKHPFFKGLNFALIRSLTPPEVPGVRRRKATPAPVAKTKDHKSGQATGFDYF 474

BLAST of Chy12G209680 vs. NCBI nr
Match: XP_038893789.1 (protein kinase PINOID [Benincasa hispida])

HSP 1 Score: 828 bits (2140), Expect = 5.49e-301
Identity = 430/481 (89.40%), Postives = 444/481 (92.31%), Query Frame = 0

Query: 1   MLQHLPPESD-------ALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLK 60
           ML+HL  ESD       A+NS SSQNSMSSESCSSFSRLSFDAAIDLPTSRSSP  L LK
Sbjct: 1   MLEHLARESDGDPGYEPAVNSTSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPECLDLK 60

Query: 61  PHRSSDFAYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDD 120
           PHRSSDFAYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLR LS+GC YEDEDD+DD
Sbjct: 61  PHRSSDFAYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRKLSEGC-YEDEDDDDD 120

Query: 121 DVDEEDDDDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEAS 180
                        CLYAMKVVDK+ALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEAS
Sbjct: 121 ------------PCLYAMKVVDKEALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEAS 180

Query: 181 HFSCIVMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPEN 240
           HFSCIVMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPEN
Sbjct: 181 HFSCIVMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPEN 240

Query: 241 VLVRSDGHIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSC 300
           VLVRSDGHIMLSDFDLSLCSDATPAVESPNSSPNT FP++TAYP+TNA V AAS  PFSC
Sbjct: 241 VLVRSDGHIMLSDFDLSLCSDATPAVESPNSSPNTAFPDATAYPRTNAAVSAASTTPFSC 300

Query: 301 LYNRLFRSRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGI 360
           LYNRLFRSRKVQT++PNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFG+
Sbjct: 301 LYNRLFRSRKVQTMTPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGV 360

Query: 361 FLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRL 420
           FLYELIYGRTPFAASSNETTLR+IIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRL
Sbjct: 361 FLYELIYGRTPFAASSNETTLRNIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRL 420

Query: 421 GSKRGSADIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDY 474
           GSKRGSADIKKHPFFKGLNFALIRSLTPPE+PGVRRR+  PAPVAK KD KSGQSTGFDY
Sbjct: 421 GSKRGSADIKKHPFFKGLNFALIRSLTPPEVPGVRRRKPTPAPVAKTKD-KSGQSTGFDY 467

BLAST of Chy12G209680 vs. NCBI nr
Match: TYJ96881.1 (protein kinase PINOID [Cucumis melo var. makuwa])

HSP 1 Score: 798 bits (2062), Expect = 1.03e-289
Identity = 416/474 (87.76%), Postives = 421/474 (88.82%), Query Frame = 0

Query: 1   MLQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60
           MLQHLP ESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF
Sbjct: 1   MLQHLPSESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF 60

Query: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDD 120
           AYSAIRRRKSKLTFRDFRLLRRIGAGDI                                
Sbjct: 61  AYSAIRRRKSKLTFRDFRLLRRIGAGDI-------------------------------- 120

Query: 121 DDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180
                       VVDK+ALELKKKV RAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM
Sbjct: 121 ------------VVDKEALELKKKVHRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVM 180

Query: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240
           EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG
Sbjct: 181 EFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDG 240

Query: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFR 300
           HIMLSDFDLSLCSDATPAVESPNSSPNT FPE+TAYPKTN  V A SAAPFSCLYNRLFR
Sbjct: 241 HIMLSDFDLSLCSDATPAVESPNSSPNTAFPETTAYPKTNPAVSATSAAPFSCLYNRLFR 300

Query: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360
           SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY
Sbjct: 301 SRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIY 360

Query: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420
           GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA
Sbjct: 361 GRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSA 420

Query: 421 DIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 474
           DIKKHPFFKGLNFALIRSLTPPE+PGVRRR+A PAPVAK KDHKSGQ+TGFDYF
Sbjct: 421 DIKKHPFFKGLNFALIRSLTPPEVPGVRRRKATPAPVAKTKDHKSGQATGFDYF 430

BLAST of Chy12G209680 vs. NCBI nr
Match: KAG7012552.1 (Protein kinase PINOID [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 780 bits (2014), Expect = 8.51e-282
Identity = 411/484 (84.92%), Postives = 432/484 (89.26%), Query Frame = 0

Query: 1   MLQHLPPESDA-------LNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLK 60
           ML+ L   SDA       +NSNSSQNSMSSESCSSFSRLSFDAA+D PTSR SP SL LK
Sbjct: 1   MLEELARGSDAELGYEAAVNSNSSQNSMSSESCSSFSRLSFDAAVDFPTSRFSPGSLDLK 60

Query: 61  PHRSSDFAYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDD 120
           PHRSSDFAYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLR L+ G  Y+DEDD+  
Sbjct: 61  PHRSSDFAYSAIRRRKSKLTFRDFRLLRRIGAGDIGTVYLCQLRKLTDGG-YDDEDDD-- 120

Query: 121 DVDEEDDDDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEAS 180
                       SCLYAMKVVDK+ALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEAS
Sbjct: 121 ------------SCLYAMKVVDKEALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEAS 180

Query: 181 HFSCIVMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPEN 240
           HFSCIVMEFCSGGDLHSLRHKQPRKRFSL+SARFYAAEVLVALEYLHMLGIIYRDLKPEN
Sbjct: 181 HFSCIVMEFCSGGDLHSLRHKQPRKRFSLSSARFYAAEVLVALEYLHMLGIIYRDLKPEN 240

Query: 241 VLVRSDGHIMLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAA---P 300
           VLVRSDGHIMLSDFDLSLCSDATPAVESP+SSPN+ FP++ AYPK+N   P ASAA   P
Sbjct: 241 VLVRSDGHIMLSDFDLSLCSDATPAVESPDSSPNSAFPDAMAYPKSNT-TPTASAATTSP 300

Query: 301 FSCLYNRLFRSRKVQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWA 360
           FSC YNRLFRSRKVQTL+PNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWA
Sbjct: 301 FSCFYNRLFRSRKVQTLTPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWA 360

Query: 361 FGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPT 420
           FG+FLYELI+GRTPFAASSNETTLR+IIKKPLTFPTA  SGALEHHARDLISGLLNKDPT
Sbjct: 361 FGVFLYELIFGRTPFAASSNETTLRNIIKKPLTFPTAETSGALEHHARDLISGLLNKDPT 420

Query: 421 RRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTG 474
           RRLGSKRGSADIKKHPFFKGLNFALIRSLTPPE+PGVRRR+  PAPVAK+ D K+  S G
Sbjct: 421 RRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEVPGVRRRKPVPAPVAKSMD-KAADSGG 467

BLAST of Chy12G209680 vs. TAIR 10
Match: AT2G34650.1 (Protein kinase superfamily protein )

HSP 1 Score: 554.3 bits (1427), Expect = 9.6e-158
Identity = 302/471 (64.12%), Postives = 354/471 (75.16%), Query Frame = 0

Query: 8   ESD---ALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDFAYSA 67
           ESD   +L + +S  S  +ESCSSFSRLSFDA     T     + L LKPHRSSDFAY+ 
Sbjct: 4   ESDGEMSLGTTNSPISSGTESCSSFSRLSFDAPPS--TIPEEESFLSLKPHRSSDFAYAE 63

Query: 68  IRRRKSK-LTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDDD 127
           IRRRK + LTFRDFRL+RRIGAGDIGTVYLC+L                       D+++
Sbjct: 64  IRRRKKQGLTFRDFRLMRRIGAGDIGTVYLCRLAG---------------------DEEE 123

Query: 128 DVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFC 187
             S  +AMKVVDK+AL LKKK+ RAEME+ ILKMLDHPFLPTLYAEFEASHFSCIVME+C
Sbjct: 124 SRSSYFAMKVVDKEALALKKKMHRAEMEKTILKMLDHPFLPTLYAEFEASHFSCIVMEYC 183

Query: 188 SGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIM 247
           SGGDLHSLRH+QP +RFSL+SARFYAAEVLVALEYLHMLGIIYRDLKPEN+LVRSDGHIM
Sbjct: 184 SGGDLHSLRHRQPHRRFSLSSARFYAAEVLVALEYLHMLGIIYRDLKPENILVRSDGHIM 243

Query: 248 LSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFRSRK 307
           LSDFDLSLCSD+  AVES +SSP  +        +  +P      A    L+ R+ RS+K
Sbjct: 244 LSDFDLSLCSDSIAAVESSSSSPENQ--------QLRSPRRFTRLAR---LFQRVLRSKK 303

Query: 308 VQTLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIYGRT 367
           VQTL P  LFVAEPV+ARS SFVGTHEYV+PEVA+GGSHGNAVDWWAFG+FLYE+IYG+T
Sbjct: 304 VQTLEPTRLFVAEPVTARSGSFVGTHEYVAPEVASGGSHGNAVDWWAFGVFLYEMIYGKT 363

Query: 368 PFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSADIK 427
           PF A +N+  LR+I+K+ L+FPT  P+   E HAR+LISGLLNKDPT+RLGS+RG+A++K
Sbjct: 364 PFVAPTNDVILRNIVKRQLSFPTDSPATMFELHARNLISGLLNKDPTKRLGSRRGAAEVK 423

Query: 428 KHPFFKGLNFALIRSLTPPEIPGVRRRRAPPAPVAKNKDHKSGQSTGFDYF 475
            HPFFKGLNFALIR+LTPPEIP    ++  P   A      S +   FDYF
Sbjct: 424 VHPFFKGLNFALIRTLTPPEIPSSVVKK--PMKSATFSGRSSNKPAAFDYF 438

BLAST of Chy12G209680 vs. TAIR 10
Match: AT5G55910.1 (D6 protein kinase )

HSP 1 Score: 381.3 bits (978), Expect = 1.1e-105
Identity = 228/497 (45.88%), Postives = 292/497 (58.75%), Query Frame = 0

Query: 2   LQHLPPESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDFA 61
           L    P SD    NS  N       SS  R S  + I   ++ SS +S   KPH+++D  
Sbjct: 31  LSFADPSSDGKTGNSKINEQGESGKSSTCRPSTSSDISDESTCSSFSSSINKPHKANDVR 90

Query: 62  YSAIRRRKSK---LTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEE 121
           + AI+  ++K   L    FRLL+R+G GDIGTV+L +L                      
Sbjct: 91  WEAIQAVRTKHGGLGLNHFRLLKRLGCGDIGTVHLAEL---------------------- 150

Query: 122 DDDDDDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCI 181
               +   C +AMKV+DK AL  +KK+ RA+ ER+IL+ LDHPFLPTLY+ FE   FSC+
Sbjct: 151 ----NGTRCYFAMKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCL 210

Query: 182 VMEFCSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRS 241
           VMEFC GGDLH+LR +QP KRF+  +A+FY AEVL+A+EYLHMLGIIYRDLKPENVLVR 
Sbjct: 211 VMEFCPGGDLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRD 270

Query: 242 DGHIMLSDFDLSL-CSDATPAVESPNSSPNTEFPESTAYPKTNAPV---PAASAAPFSCL 301
           DGH+MLSDFDLSL C+ +   V S N         S +  +  A +      S AP SC 
Sbjct: 271 DGHVMLSDFDLSLRCTVSLSIVRSANVGSEGLSKNSVSCSQQPACIQQPSCISMAPTSCF 330

Query: 302 YNRLFRSRKVQTLSPNW-----------LFVAEPVSARSCSFVGTHEYVSPEVAAGGSHG 361
             R F S+  +   P               VAEP  ARS SFVGTHEY++PE+  G  HG
Sbjct: 331 GPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEGHG 390

Query: 362 NAVDWWAFGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISG 421
           +AVDWW FGIFLYEL++G+TPF  S N  TL +++ +PL FP    S  +   ARDLI  
Sbjct: 391 SAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFP---ESPVVSFAARDLIRS 450

Query: 422 LLNKDPTRRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEIPGVRRRRA----PPAPVAK 475
           LL K+P  RL  KRG+ +IK+HPFF+G+N+AL+R  +PPEIP      A    P  P A 
Sbjct: 451 LLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEIPKPVDLEALNPTPTVPAAA 498

BLAST of Chy12G209680 vs. TAIR 10
Match: AT5G40030.1 (Protein kinase superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 5.5e-105
Identity = 218/490 (44.49%), Postives = 289/490 (58.98%), Query Frame = 0

Query: 8   ESDALNSNSSQNSMSSESCSSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDFAYSAIRR 67
           E   LN++ S +  SS S SS + L    + D   +         KPH+ +D  + AI+ 
Sbjct: 41  EDVRLNNSISLSFCSSNSVSSEANLEKTQSFDANEANFKRVFAPSKPHKGNDLRWDAIQN 100

Query: 68  ----RKSKLTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDDD 127
               +   L    FRLL+++G GDIG+VYL +LR                          
Sbjct: 101 VKCSKNEDLGLGHFRLLKKLGCGDIGSVYLAELR-------------------------- 160

Query: 128 DVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFC 187
           ++ C +AMKV+DK  L  +KK+ RA+ ER+IL +LDHPFLPTLY+ FE   FSC++MEFC
Sbjct: 161 EMGCFFAMKVMDKGMLIGRKKLVRAQTEREILGLLDHPFLPTLYSHFETEKFSCLLMEFC 220

Query: 188 SGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIM 247
           SGGDLH LR KQP K FS  +ARFYA+EVL+ALEYLHM+G++YRDLKPENV+VR DGHIM
Sbjct: 221 SGGDLHILRQKQPGKHFSELAARFYASEVLLALEYLHMMGVVYRDLKPENVMVREDGHIM 280

Query: 248 LSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAP---VPAASAAPFSCLYNRLF- 307
           LSDFDLSL S  +P +    S P+         P    P   +P A   P SC   R   
Sbjct: 281 LSDFDLSLQSFVSPTLIQSTSQPSCHIASYCIQPPCIDPSCKLPVACIQP-SCFKPRFLN 340

Query: 308 -RSRKVQTLSPNW----LFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIF 367
            + RK +T         + +AEP +ARS SFVGTHEY++PE+  G  HG++VDWW FGIF
Sbjct: 341 NKPRKAKTEKAGSDSLPMLIAEPTAARSMSFVGTHEYLAPEIIRGDGHGSSVDWWTFGIF 400

Query: 368 LYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPTRRLG 427
           LYEL+ G+TPF  + N  TL +++ +PL F    P G++   A+DLI GLL KDP +RLG
Sbjct: 401 LYELLTGKTPFKGNGNRETLFNVVGQPLKF----PEGSISFAAKDLIRGLLTKDPKKRLG 460

Query: 428 SKRGSADIKKHPFFKGLNFALIRSLTPPEIP----------GVRRRRAPPAPVAKNKDHK 475
            K+G+ +IK+HPFF  +N+ALIRS TPPEIP           ++         +K  D  
Sbjct: 461 FKKGATEIKQHPFFNNVNWALIRSTTPPEIPKPIDLSILNETLKSSVQQQGKHSKQSDSS 499

BLAST of Chy12G209680 vs. TAIR 10
Match: AT4G26610.1 (D6 protein kinase like 1 )

HSP 1 Score: 375.9 bits (964), Expect = 4.7e-104
Identity = 223/488 (45.70%), Postives = 295/488 (60.45%), Query Frame = 0

Query: 7   PESDALNSNSSQNSMSSESCSSFSRLSFDAAIDL-PTSRSSPASLHLKPHRSSDFAYSAI 66
           P SD+      + +  S+     +      + D+   S  S  S + KPH+++D  + AI
Sbjct: 49  PSSDSKTGGEVKFNEKSDQSGKSNTCRPSTSSDISDESTCSSFSGNNKPHKANDVRWEAI 108

Query: 67  RRRKSK---LTFRDFRLLRRIGAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDD 126
           +  ++K   L    FRLL+R+G GDIGTV+L +L                          
Sbjct: 109 QAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLAELHG------------------------ 168

Query: 127 DDVSCLYAMKVVDKDALELKKKVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEF 186
               C +AMKV+DK AL  +KK+ RA+ ER+IL+ LDHPFLPTLY+ FE   FSC+VMEF
Sbjct: 169 --TRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEF 228

Query: 187 CSGGDLHSLRHKQPRKRFSLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHI 246
           C GGDLH+LR +QP KRFS  +A+FY AEVL+A+EYLHMLGIIYRDLKPENVLVR DGH+
Sbjct: 229 CPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHV 288

Query: 247 MLSDFDLSLCSDATPAVESPNSSPNTEFPESTAYPKTNAPV--PAASAAPFSCLYNRLFR 306
           MLSDFDLSL    +P V   ++   +E  +++ Y    A +  P+  +AP +C   R F 
Sbjct: 289 MLSDFDLSLRCTVSPTVVR-STVLASEGQKNSGYCAQPACIQQPSCISAPTTCFSPRYFS 348

Query: 307 SRKVQ----------TLSPNWLFVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWA 366
           S+  +           +SP    VAEP SARS SFVGTHEY++PE+  G  HG+AVDWW 
Sbjct: 349 SKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVDWWT 408

Query: 367 FGIFLYELIYGRTPFAASSNETTLRSIIKKPLTFPTAVPSGALEHHARDLISGLLNKDPT 426
           FGIFLYEL++G+TPF  S N  TL +++ +PL FP    S  +   ARDLI  LL K+P 
Sbjct: 409 FGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFP---ESPVVSFAARDLIRSLLVKEPQ 468

Query: 427 RRLGSKRGSADIKKHPFFKGLNFALIRSLTPPEIP-GVRRRRAPPAPVAKNKDH-KSGQS 475
            RL  KRG+ ++K+HPFF+G+N+AL+R  +PPEIP  V    AP  P A      KS QS
Sbjct: 469 HRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIPKPVDYESAPATPAAATSTSVKSDQS 506

BLAST of Chy12G209680 vs. TAIR 10
Match: AT5G47750.1 (D6 protein kinase like 2 )

HSP 1 Score: 369.8 bits (948), Expect = 3.3e-102
Identity = 216/439 (49.20%), Postives = 272/439 (61.96%), Query Frame = 0

Query: 27  SSFSRLSFDAAIDLPTSRSSPASLHLKPHRSSDF---AYSAIRRRKSKLTFRDFRLLRRI 86
           SS  R S  + +   +S SS +S   KPH+++D    A  A+R R   L    FRLL+R+
Sbjct: 138 SSICRGSTSSDVSDESSCSSFSSTVNKPHKANDLRWEAIQAVRVRDGLLGLSHFRLLKRL 197

Query: 87  GAGDIGTVYLCQLRNLSKGCCYEDEDDEDDDVDEEDDDDDDVSCLYAMKVVDKDALELKK 146
           G GDIG+VYL +L                              C +AMKV+DK +L  +K
Sbjct: 198 GCGDIGSVYLSEL--------------------------SGTKCYFAMKVMDKTSLASRK 257

Query: 147 KVQRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFCSGGDLHSLRHKQPRKRFSLT 206
           K+ RA+ ER+IL+ LDHPFLPTLY  FE   FSC+VMEFC GGDLH+LR +QP K FS  
Sbjct: 258 KLLRAQTEREILQSLDHPFLPTLYTHFETEKFSCLVMEFCPGGDLHTLRQRQPGKHFSEQ 317

Query: 207 SARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIMLSDFDLSLCSDATP------ 266
           + +FY AE L+ALEYLHMLGI+YRDLKPENVLVR DGHIMLSDFDLSL    +P      
Sbjct: 318 AVKFYIAESLLALEYLHMLGIVYRDLKPENVLVREDGHIMLSDFDLSLRCLVSPTLVKSA 377

Query: 267 AVESPNSSPNTEFPESTAYPKTNAPVPAASAAPFSCLYNRLFRS-----RKVQTLSPNWL 326
           A+ES     N    +        + +  +   P +C   RLF S     RK +  + N +
Sbjct: 378 AIESDPLRKNVYCVQPACIEP--SCIQPSCTVPTTCFSPRLFSSKSKKDRKPKNDTANQV 437

Query: 327 -----FVAEPVSARSCSFVGTHEYVSPEVAAGGSHGNAVDWWAFGIFLYELIYGRTPFAA 386
                 VAEP  ARS SFVGTHEY++PE+  G  HG+AVDWW FGIFLYEL++GRTPF  
Sbjct: 438 RPLPELVAEPTDARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKG 497

Query: 387 SSNETTLRSIIKKPLTFP-TAVPSGALEHHARDLISGLLNKDPTRRLGSKRGSADIKKHP 446
           S N  TL +++ +PL FP T V S A    ARDLI GLL K+P +RLG KRG+ ++K+HP
Sbjct: 498 SGNRQTLFNVVGQPLRFPETPVVSFA----ARDLIRGLLMKEPQQRLGFKRGATEVKQHP 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O646821.3e-15664.12Protein kinase PINOID OS=Arabidopsis thaliana OX=3702 GN=PID PE=1 SV=1[more]
Q2QM771.7e-12250.72Protein kinase PINOID OS=Oryza sativa subsp. japonica OX=39947 GN=PID PE=2 SV=1[more]
Q94E491.3e-10646.59Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV... [more]
Q9FG741.6e-10445.88Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=... [more]
Q9SUA36.6e-10345.70Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1... [more]
Match NameE-valueIdentityDescription
A0A0A0LVF02.5e-26297.89Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537... [more]
A0A5A7UE242.8e-25895.99Protein kinase PINOID OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold31... [more]
A0A1S3BC272.8e-25895.99protein kinase PINOID OS=Cucumis melo OX=3656 GN=LOC103488038 PE=4 SV=1[more]
A0A5D3BDZ96.1e-22987.76Protein kinase PINOID OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10... [more]
A0A6J1GR981.5e-22286.94protein kinase PINOID-like OS=Cucurbita moschata OX=3662 GN=LOC111456752 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_004135537.10.097.89protein kinase PINOID [Cucumis sativus] >KGN65890.1 hypothetical protein Csa_023... [more]
XP_008444803.10.095.99PREDICTED: protein kinase PINOID [Cucumis melo] >KAA0054042.1 protein kinase PIN... [more]
XP_038893789.15.49e-30189.40protein kinase PINOID [Benincasa hispida][more]
TYJ96881.11.03e-28987.76protein kinase PINOID [Cucumis melo var. makuwa][more]
KAG7012552.18.51e-28284.92Protein kinase PINOID [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT2G34650.19.6e-15864.12Protein kinase superfamily protein [more]
AT5G55910.11.1e-10545.88D6 protein kinase [more]
AT5G40030.15.5e-10544.49Protein kinase superfamily protein [more]
AT4G26610.14.7e-10445.70D6 protein kinase like 1 [more]
AT5G47750.13.3e-10249.20D6 protein kinase like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 131..158
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 75..182
e-value: 3.5E-45
score: 156.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 183..250
e-value: 3.5E-45
score: 156.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 322..446
e-value: 7.7E-36
score: 125.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 444..474
NoneNo IPR availablePANTHERPTHR45637FLIPPASE KINASE 1-RELATEDcoord: 14..472
NoneNo IPR availablePANTHERPTHR45637:SF75SERINE/THREONINE KINASE FAMILY PROTEINcoord: 14..472
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 77..428
e-value: 8.4E-84
score: 294.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 126..257
e-value: 1.1E-29
score: 103.6
coord: 322..428
e-value: 6.4E-18
score: 65.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 77..428
score: 42.067745
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 224..236
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 56..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G209680.1Chy12G209680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0009926 auxin polar transport
biological_process GO:0048825 cotyledon development
biological_process GO:0009958 positive gravitropism
biological_process GO:0006468 protein phosphorylation
biological_process GO:0048767 root hair elongation
biological_process GO:0048766 root hair initiation
cellular_component GO:0009986 cell surface
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity