Chy12G206110 (gene) Cucumber (hystrix) v1

BLAST of Chy12G206110 vs. ExPASy Swiss-Prot
Match: F4I1G5 (Cold-regulated 413 inner membrane protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=COR413IM2 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 3.6e-59
Identity = 130/231 (56.28%), Postives = 162/231 (70.13%), Query Frame = 0

Query: 2   VCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRF------ 61
           +C+S S  VSL     +  K  + L LR  P N           S +C+NPLR       
Sbjct: 4   LCLSSSRIVSL-----HHQKPFLALKLRPRPSNIS---GLGHSTSVVCFNPLRLSADRQR 63

Query: 62  -AVGSEGINKKKSRGLSAVCYAM-LVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFA 121
            A  S    K++ RG S VCYA  +++   LQWISTIS V LM A+GTGI KSF+VPLFA
Sbjct: 64  TATVSARAEKRRKRGSSVVCYATPMLSVHNLQWISTISCVALMFARGTGIHKSFVVPLFA 123

Query: 122 LQAPASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQ 181
           LQAP  ++SW+KGEYGIW+AFLALL RLFF  P ELE+PFI+LLL+IVAPYQV ++RG Q
Sbjct: 124 LQAPMGIVSWMKGEYGIWAAFLALLTRLFFSFPVELELPFIALLLVIVAPYQVMSIRGKQ 183

Query: 182 EGCIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           EG I+SL I+ +LAFQHFSRAG+ Q+AFDQNS++ATVA+I +T VSFLF+I
Sbjct: 184 EGAILSLAISCFLAFQHFSRAGTLQKAFDQNSVLATVAIIGVTVVSFLFLI 226

BLAST of Chy12G206110 vs. ExPASy Swiss-Prot
Match: Q94AL8 (Cold-regulated 413 inner membrane protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=COR413IM1 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 6.8e-58
Identity = 122/231 (52.81%), Postives = 167/231 (72.29%), Query Frame = 0

Query: 2   VCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSS-ICYNPLRF----- 61
           +C+S S  VSL     +  K  + L LRS P +    L+   + +S +C+NPLR      
Sbjct: 4   LCLSSSRIVSL-----HHQKPFLSLKLRSRPSD----LSGLGRHTSPVCFNPLRLSGDRQ 63

Query: 62  --AVGSEGINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFA 121
             A  S  + K++ RG S VCYA  ++  +LQWISTIS + LMLA+GTGI KS +VPLFA
Sbjct: 64  RTATVSTRVEKRRKRGSSVVCYAAPISANSLQWISTISCLALMLARGTGIHKSVVVPLFA 123

Query: 122 LQAPASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQ 181
           L AP+S+++WIKGEYG+W+AFLAL+ RLFF  PGELE+PFI+LLL+IVAPYQV N+RG Q
Sbjct: 124 LHAPSSIVAWIKGEYGVWAAFLALIARLFFTFPGELELPFIALLLVIVAPYQVMNIRGKQ 183

Query: 182 EGCIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           EG II++ I+ +LAFQHFSRAGS ++A+++ S++ATVA+I +T VS L ++
Sbjct: 184 EGAIIAIAISGFLAFQHFSRAGSLEKAYEKGSVLATVAIIGVTVVSLLLLL 225

BLAST of Chy12G206110 vs. ExPASy Swiss-Prot
Match: O23164 (Cold-regulated 413 plasma membrane protein 4 OS=Arabidopsis thaliana OX=3702 GN=At4g37220 PE=2 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 2.5e-12
Identity = 45/143 (31.47%), Postives = 79/143 (55.24%), Query Frame = 0

Query: 81  TRTLQWISTISSVVLMLAKGTG----IQKSFIVPLFALQAPASVISWIKGEYGIWSAFLA 140
           T  LQW ++I ++ L++   T     +  + +VP      P+ +  +  G++G W A +A
Sbjct: 47  TSVLQWAASIFAIYLLILDRTNWKTKMLTTLLVPYIFFTLPSVIFQFFSGDFGKWIALIA 106

Query: 141 LLVRLFF--FIPGELEIPFISLLLMIVAPYQVQ-NLRGTQEGCIISLLIAAYLAFQHFSR 200
           ++VRLFF    P  LEIP   +L+++V+P  +   LR +  G +I L+IA YL  +H   
Sbjct: 107 IIVRLFFPKEFPEWLEIPVALILIVVVSPSLIAWTLRESWVGAVICLVIACYLFHEHIKA 166

Query: 201 AGSFQRAFDQ-NSIVATVAVICI 216
           +G F+ +F Q N I  T+ ++ +
Sbjct: 167 SGGFKNSFTQKNGISNTIGIVAL 189

BLAST of Chy12G206110 vs. ExPASy Swiss-Prot
Match: Q9SVL6 (Cold-regulated 413 plasma membrane protein 2 OS=Arabidopsis thaliana OX=3702 GN=COR413PM2 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.1e-11
Identity = 42/140 (30.00%), Postives = 82/140 (58.57%), Query Frame = 0

Query: 84  LQWISTISSVVLMLAKGTG----IQKSFIVPLFALQAPASVISWIKGEYGIWSAFLALLV 143
           L+++++ +++ L++   T     +  S ++P   L  P+ + +++ G+ G W AF+A+++
Sbjct: 51  LKFLASFAAIYLLILDRTNWKTKMLTSLLIPYIFLSLPSVIFNFLSGDVGKWIAFVAVVL 110

Query: 144 RLFF--FIPGELEIPFISLLLMIVAP-YQVQNLRGTQEGCIISLLIAAYLAFQHFSRAGS 203
           RLFF    P  LE+P   +LL++V+P +   ++RGT  G +ISL I  YL  +H   +G 
Sbjct: 111 RLFFPKHFPDWLEMPGSLILLLVVSPHFLAHHIRGTWIGTVISLFIGCYLLQEHIRASGG 170

Query: 204 FQRAFDQ-NSIVATVAVICI 216
           F+ +F Q   +  T+ +I +
Sbjct: 171 FRNSFTQPRGVSNTLGIILL 190

BLAST of Chy12G206110 vs. ExPASy Swiss-Prot
Match: Q9XIM7 (Cold-regulated 413 plasma membrane protein 1 OS=Arabidopsis thaliana OX=3702 GN=COR413PM1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 46/143 (32.17%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 81  TRTLQWISTISSVVLMLAK----GTGIQKSFIVPLFALQAPASVISWIKGEYGIWSAFLA 140
           T  L+W+++I+++ L++       T +  S ++P      P+ +    +GE G W AF+A
Sbjct: 45  TSVLEWVASIAAIYLLVLDRTNWKTNMLTSLLIPYIFFSLPSLIFGIFRGEIGKWIAFVA 104

Query: 141 LLVRLFF--FIPGELEIPFISLLLMIVAPYQVQ-NLRGTQEGCIISLLIAAYLAFQHFSR 200
           ++V+LFF       LE+P   +LL +VAP  +    R +  G  I L I  YL  +H   
Sbjct: 105 VVVQLFFPKHAREYLELPVALVLLAVVAPNLIAGTFRDSWIGLAICLGIGCYLLQEHIRA 164

Query: 201 AGSFQRAFDQ-NSIVATVAVICI 216
           +G F+ AF + N I  TV +IC+
Sbjct: 165 SGGFRNAFTKANGISNTVGIICL 187

BLAST of Chy12G206110 vs. ExPASy TrEMBL
Match: A0A0A0LXM7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G459500 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.5e-111
Identity = 219/224 (97.77%), Postives = 221/224 (98.66%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCVSLSSAVSLY P FYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE
Sbjct: 1   MVCVSLSSAVSLYIPSFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60

Query: 61  GINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQAPASV 120
           GINKKKSRGLSAVCYAM VNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQAPASV
Sbjct: 61  GINKKKSRGLSAVCYAMPVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQAPASV 120

Query: 121 ISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEGCIISL 180
           ISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEGCIISL
Sbjct: 121 ISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEGCIISL 180

Query: 181 LIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           LIAAYLAFQHFSRAGSFQRAFDQNSIVATVAV+CITAVSFLFVI
Sbjct: 181 LIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVVCITAVSFLFVI 224

BLAST of Chy12G206110 vs. ExPASy TrEMBL
Match: A0A5D3DB21 (Cold-regulated 413 inner membrane protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00120 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.5e-108
Identity = 217/229 (94.76%), Postives = 220/229 (96.07%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCVSLSSAVSLY P FYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE
Sbjct: 1   MVCVSLSSAVSLYPPSFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60

Query: 61  -----GINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
                GINKKK+RGLSA CYAM VNTRTLQW+STISSVVLMLAKGTGIQKSFIVPLFALQ
Sbjct: 61  GIATAGINKKKNRGLSAACYAMPVNTRTLQWVSTISSVVLMLAKGTGIQKSFIVPLFALQ 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEG
Sbjct: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI
Sbjct: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 229

BLAST of Chy12G206110 vs. ExPASy TrEMBL
Match: A0A1S3B961 (cold-regulated 413 inner membrane protein 2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103487404 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.5e-108
Identity = 217/229 (94.76%), Postives = 220/229 (96.07%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCVSLSSAVSLY P FYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE
Sbjct: 1   MVCVSLSSAVSLYPPSFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60

Query: 61  -----GINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
                GINKKK+RGLSA CYAM VNTRTLQW+STISSVVLMLAKGTGIQKSFIVPLFALQ
Sbjct: 61  GIATAGINKKKNRGLSAACYAMPVNTRTLQWVSTISSVVLMLAKGTGIQKSFIVPLFALQ 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEG
Sbjct: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI
Sbjct: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 229

BLAST of Chy12G206110 vs. ExPASy TrEMBL
Match: A0A6J1K0I6 (cold-regulated 413 inner membrane protein 1, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490472 PE=3 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.9e-93
Identity = 191/229 (83.41%), Postives = 207/229 (90.39%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCV LSSA+SL++P  +KSKLS PLSLRSS PNAKL+ N AAKQSSIC NP+RF  GS+
Sbjct: 1   MVCV-LSSAISLHSPFLHKSKLSAPLSLRSSLPNAKLTFNPAAKQSSICSNPIRFGFGSD 60

Query: 61  -----GINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
                 INKK+ RGLSAVCYAM ++ R LQWISTISSV+LMLA+GTGIQKSFIVPLFALQ
Sbjct: 61  VSSTLAINKKRRRGLSAVCYAMPLSARNLQWISTISSVILMLARGTGIQKSFIVPLFALQ 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEG
Sbjct: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
            IISLLIAAYLAFQHFSRAGSF+RAFDQNSIVATVA+IC+TAVSFL VI
Sbjct: 181 TIISLLIAAYLAFQHFSRAGSFRRAFDQNSIVATVAIICVTAVSFLLVI 228

BLAST of Chy12G206110 vs. ExPASy TrEMBL
Match: A0A6J1CH44 (cold-regulated 413 inner membrane protein 1, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010803 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.0e-92
Identity = 187/229 (81.66%), Postives = 205/229 (89.52%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVC+SLSSA SL+T   YK+KLS PLSLRS P +AKLSLN A K+SSICYNPLRFAVG+E
Sbjct: 1   MVCLSLSSATSLHTLSLYKTKLSAPLSLRSPPLHAKLSLNPAVKRSSICYNPLRFAVGNE 60

Query: 61  G-----INKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
           G     INK++SRGL+ VCYAM +  R +QWISTIS VVLMLA+GTGIQKSFIVPLFALQ
Sbjct: 61  GIATAAINKRRSRGLTTVCYAMPLTARNIQWISTISYVVLMLARGTGIQKSFIVPLFALQ 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASVISWIKGEYGIW+AFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEG
Sbjct: 121 APASVISWIKGEYGIWTAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
            IISLLIAA+LAFQHFSRAGSF+RAFDQNSIVAT AV+C+TA SFL VI
Sbjct: 181 SIISLLIAAFLAFQHFSRAGSFRRAFDQNSIVATAAVVCVTAASFLLVI 229

BLAST of Chy12G206110 vs. NCBI nr
Match: XP_004147401.1 (cold-regulated 413 inner membrane protein 2, chloroplastic [Cucumis sativus] >KGN65572.1 hypothetical protein Csa_019760 [Cucumis sativus])

HSP 1 Score: 407 bits (1047), Expect = 1.07e-142
Identity = 219/224 (97.77%), Postives = 221/224 (98.66%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCVSLSSAVSLY P FYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE
Sbjct: 1   MVCVSLSSAVSLYIPSFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60

Query: 61  GINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQAPASV 120
           GINKKKSRGLSAVCYAM VNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQAPASV
Sbjct: 61  GINKKKSRGLSAVCYAMPVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQAPASV 120

Query: 121 ISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEGCIISL 180
           ISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEGCIISL
Sbjct: 121 ISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEGCIISL 180

Query: 181 LIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 224
           LIAAYLAFQHFSRAGSFQRAFDQNSIVATVAV+CITAVSFLFVI
Sbjct: 181 LIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVVCITAVSFLFVI 224

BLAST of Chy12G206110 vs. NCBI nr
Match: XP_008443932.1 (PREDICTED: cold-regulated 413 inner membrane protein 2, chloroplastic-like [Cucumis melo] >KAA0057095.1 cold-regulated 413 inner membrane protein 2 [Cucumis melo var. makuwa] >TYK20630.1 cold-regulated 413 inner membrane protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 399 bits (1024), Expect = 4.10e-139
Identity = 217/229 (94.76%), Postives = 220/229 (96.07%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCVSLSSAVSLY P FYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE
Sbjct: 1   MVCVSLSSAVSLYPPSFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60

Query: 61  GI-----NKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
           GI     NKKK+RGLSA CYAM VNTRTLQW+STISSVVLMLAKGTGIQKSFIVPLFALQ
Sbjct: 61  GIATAGINKKKNRGLSAACYAMPVNTRTLQWVSTISSVVLMLAKGTGIQKSFIVPLFALQ 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEG
Sbjct: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 224
           CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI
Sbjct: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 229

BLAST of Chy12G206110 vs. NCBI nr
Match: XP_038896035.1 (cold-regulated 413 inner membrane protein 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 370 bits (950), Expect = 7.72e-128
Identity = 202/229 (88.21%), Postives = 211/229 (92.14%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCVSLSSAVSLY P  Y+SKLSVPLSLRSSPPNAKLS + AAKQSSICYNPLRFAVGSE
Sbjct: 1   MVCVSLSSAVSLYNPSLYRSKLSVPLSLRSSPPNAKLSFSPAAKQSSICYNPLRFAVGSE 60

Query: 61  GI-----NKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
           GI      KK+SRGLSA+CYAM VN RTLQWISTISS VLMLAKGTGIQKSFIVPLFAL 
Sbjct: 61  GIATAAITKKRSRGLSAICYAMPVNARTLQWISTISSAVLMLAKGTGIQKSFIVPLFALL 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASV+SWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQ+LRGTQEG
Sbjct: 121 APASVVSWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQSLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 224
            IISL+IAAYLAFQHFSRAGSF+RAFDQNSIVATVAVIC TAVSFL VI
Sbjct: 181 NIISLMIAAYLAFQHFSRAGSFRRAFDQNSIVATVAVICFTAVSFLLVI 229

BLAST of Chy12G206110 vs. NCBI nr
Match: XP_022994881.1 (cold-regulated 413 inner membrane protein 1, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 348 bits (893), Expect = 3.58e-119
Identity = 191/229 (83.41%), Postives = 207/229 (90.39%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCV LSSA+SL++P  +KSKLS PLSLRSS PNAKL+ N AAKQSSIC NP+RF  GS+
Sbjct: 1   MVCV-LSSAISLHSPFLHKSKLSAPLSLRSSLPNAKLTFNPAAKQSSICSNPIRFGFGSD 60

Query: 61  -----GINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
                 INKK+ RGLSAVCYAM ++ R LQWISTISSV+LMLA+GTGIQKSFIVPLFALQ
Sbjct: 61  VSSTLAINKKRRRGLSAVCYAMPLSARNLQWISTISSVILMLARGTGIQKSFIVPLFALQ 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEG
Sbjct: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 224
            IISLLIAAYLAFQHFSRAGSF+RAFDQNSIVATVA+IC+TAVSFL VI
Sbjct: 181 TIISLLIAAYLAFQHFSRAGSFRRAFDQNSIVATVAIICVTAVSFLLVI 228

BLAST of Chy12G206110 vs. NCBI nr
Match: XP_022955155.1 (cold-regulated 413 inner membrane protein 1, chloroplastic-like isoform X2 [Cucurbita moschata] >KAG6573346.1 Cold-regulated 413 inner membrane protein 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012511.1 Cold-regulated inner membrane protein 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 346 bits (887), Expect = 2.94e-118
Identity = 190/229 (82.97%), Postives = 205/229 (89.52%), Query Frame = 0

Query: 1   MVCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRFAVGSE 60
           MVCV LSSA+SL++P  YKSKLS PLSLRSS PNAKL+ N AAKQS IC NP+RF  GS+
Sbjct: 1   MVCV-LSSAISLHSPVLYKSKLSAPLSLRSSLPNAKLTFNPAAKQSLICSNPIRFGFGSD 60

Query: 61  -----GINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFALQ 120
                 INKK+ RGLSAVCYAM ++ R LQWISTISSV+LMLA+GTGIQKSFIVPLFALQ
Sbjct: 61  VSSTLAINKKRRRGLSAVCYAMPLSARNLQWISTISSVILMLARGTGIQKSFIVPLFALQ 120

Query: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQEG 180
           APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLL+IVAPYQVQNLRGTQEG
Sbjct: 121 APASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLVIVAPYQVQNLRGTQEG 180

Query: 181 CIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 224
            IISLLIAAYLAFQHFSRAGSF+RAFDQNSIVATVA+IC+TAVS L VI
Sbjct: 181 TIISLLIAAYLAFQHFSRAGSFRRAFDQNSIVATVAIICVTAVSILLVI 228

BLAST of Chy12G206110 vs. TAIR 10
Match: AT1G29390.1 (cold regulated 314 thylakoid membrane 2 )

HSP 1 Score: 229.6 bits (584), Expect = 2.6e-60
Identity = 130/231 (56.28%), Postives = 162/231 (70.13%), Query Frame = 0

Query: 2   VCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSSICYNPLRF------ 61
           +C+S S  VSL     +  K  + L LR  P N           S +C+NPLR       
Sbjct: 4   LCLSSSRIVSL-----HHQKPFLALKLRPRPSNIS---GLGHSTSVVCFNPLRLSADRQR 63

Query: 62  -AVGSEGINKKKSRGLSAVCYAM-LVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFA 121
            A  S    K++ RG S VCYA  +++   LQWISTIS V LM A+GTGI KSF+VPLFA
Sbjct: 64  TATVSARAEKRRKRGSSVVCYATPMLSVHNLQWISTISCVALMFARGTGIHKSFVVPLFA 123

Query: 122 LQAPASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQ 181
           LQAP  ++SW+KGEYGIW+AFLALL RLFF  P ELE+PFI+LLL+IVAPYQV ++RG Q
Sbjct: 124 LQAPMGIVSWMKGEYGIWAAFLALLTRLFFSFPVELELPFIALLLVIVAPYQVMSIRGKQ 183

Query: 182 EGCIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           EG I+SL I+ +LAFQHFSRAG+ Q+AFDQNS++ATVA+I +T VSFLF+I
Sbjct: 184 EGAILSLAISCFLAFQHFSRAGTLQKAFDQNSVLATVAIIGVTVVSFLFLI 226

BLAST of Chy12G206110 vs. TAIR 10
Match: AT1G29395.1 (COLD REGULATED 314 INNER MEMBRANE 1 )

HSP 1 Score: 225.3 bits (573), Expect = 4.8e-59
Identity = 122/231 (52.81%), Postives = 167/231 (72.29%), Query Frame = 0

Query: 2   VCVSLSSAVSLYTPCFYKSKLSVPLSLRSSPPNAKLSLNSAAKQSS-ICYNPLRF----- 61
           +C+S S  VSL     +  K  + L LRS P +    L+   + +S +C+NPLR      
Sbjct: 4   LCLSSSRIVSL-----HHQKPFLSLKLRSRPSD----LSGLGRHTSPVCFNPLRLSGDRQ 63

Query: 62  --AVGSEGINKKKSRGLSAVCYAMLVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLFA 121
             A  S  + K++ RG S VCYA  ++  +LQWISTIS + LMLA+GTGI KS +VPLFA
Sbjct: 64  RTATVSTRVEKRRKRGSSVVCYAAPISANSLQWISTISCLALMLARGTGIHKSVVVPLFA 123

Query: 122 LQAPASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGTQ 181
           L AP+S+++WIKGEYG+W+AFLAL+ RLFF  PGELE+PFI+LLL+IVAPYQV N+RG Q
Sbjct: 124 LHAPSSIVAWIKGEYGVWAAFLALIARLFFTFPGELELPFIALLLVIVAPYQVMNIRGKQ 183

Query: 182 EGCIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           EG II++ I+ +LAFQHFSRAGS ++A+++ S++ATVA+I +T VS L ++
Sbjct: 184 EGAIIAIAISGFLAFQHFSRAGSLEKAYEKGSVLATVAIIGVTVVSLLLLL 225

BLAST of Chy12G206110 vs. TAIR 10
Match: AT1G29390.2 (cold regulated 314 thylakoid membrane 2 )

HSP 1 Score: 221.1 bits (562), Expect = 9.1e-58
Identity = 113/172 (65.70%), Postives = 139/172 (80.81%), Query Frame = 0

Query: 54  RFAVGSEGINKKKSRGLSAVCYAM-LVNTRTLQWISTISSVVLMLAKGTGIQKSFIVPLF 113
           R A  S    K++ RG S VCYA  +++   LQWISTIS V LM A+GTGI KSF+VPLF
Sbjct: 12  RTATVSARAEKRRKRGSSVVCYATPMLSVHNLQWISTISCVALMFARGTGIHKSFVVPLF 71

Query: 114 ALQAPASVISWIKGEYGIWSAFLALLVRLFFFIPGELEIPFISLLLMIVAPYQVQNLRGT 173
           ALQAP  ++SW+KGEYGIW+AFLALL RLFF  P ELE+PFI+LLL+IVAPYQV ++RG 
Sbjct: 72  ALQAPMGIVSWMKGEYGIWAAFLALLTRLFFSFPVELELPFIALLLVIVAPYQVMSIRGK 131

Query: 174 QEGCIISLLIAAYLAFQHFSRAGSFQRAFDQNSIVATVAVICITAVSFLFVI 225
           QEG I+SL I+ +LAFQHFSRAG+ Q+AFDQNS++ATVA+I +T VSFLF+I
Sbjct: 132 QEGAILSLAISCFLAFQHFSRAGTLQKAFDQNSVLATVAIIGVTVVSFLFLI 183

BLAST of Chy12G206110 vs. TAIR 10
Match: AT4G37220.1 (Cold acclimation protein WCOR413 family )

HSP 1 Score: 73.9 bits (180), Expect = 1.8e-13
Identity = 45/143 (31.47%), Postives = 79/143 (55.24%), Query Frame = 0

Query: 81  TRTLQWISTISSVVLMLAKGTG----IQKSFIVPLFALQAPASVISWIKGEYGIWSAFLA 140
           T  LQW ++I ++ L++   T     +  + +VP      P+ +  +  G++G W A +A
Sbjct: 47  TSVLQWAASIFAIYLLILDRTNWKTKMLTTLLVPYIFFTLPSVIFQFFSGDFGKWIALIA 106

Query: 141 LLVRLFF--FIPGELEIPFISLLLMIVAPYQVQ-NLRGTQEGCIISLLIAAYLAFQHFSR 200
           ++VRLFF    P  LEIP   +L+++V+P  +   LR +  G +I L+IA YL  +H   
Sbjct: 107 IIVRLFFPKEFPEWLEIPVALILIVVVSPSLIAWTLRESWVGAVICLVIACYLFHEHIKA 166

Query: 201 AGSFQRAFDQ-NSIVATVAVICI 216
           +G F+ +F Q N I  T+ ++ +
Sbjct: 167 SGGFKNSFTQKNGISNTIGIVAL 189

BLAST of Chy12G206110 vs. TAIR 10
Match: AT3G50830.1 (cold-regulated 413-plasma membrane 2 )

HSP 1 Score: 70.9 bits (172), Expect = 1.5e-12
Identity = 42/140 (30.00%), Postives = 82/140 (58.57%), Query Frame = 0

Query: 84  LQWISTISSVVLMLAKGTG----IQKSFIVPLFALQAPASVISWIKGEYGIWSAFLALLV 143
           L+++++ +++ L++   T     +  S ++P   L  P+ + +++ G+ G W AF+A+++
Sbjct: 51  LKFLASFAAIYLLILDRTNWKTKMLTSLLIPYIFLSLPSVIFNFLSGDVGKWIAFVAVVL 110

Query: 144 RLFF--FIPGELEIPFISLLLMIVAP-YQVQNLRGTQEGCIISLLIAAYLAFQHFSRAGS 203
           RLFF    P  LE+P   +LL++V+P +   ++RGT  G +ISL I  YL  +H   +G 
Sbjct: 111 RLFFPKHFPDWLEMPGSLILLLVVSPHFLAHHIRGTWIGTVISLFIGCYLLQEHIRASGG 170

Query: 204 FQRAFDQ-NSIVATVAVICI 216
           F+ +F Q   +  T+ +I +
Sbjct: 171 FRNSFTQPRGVSNTLGIILL 190