Chy11G203370 (gene) Cucumber (hystrix) v1

Overview
NameChy11G203370
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAutophagy-related protein 3
LocationchrH11: 23959911 .. 23965220 (-)
RNA-Seq ExpressionChy11G203370
SyntenyChy11G203370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTTATCCCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTTGAGAGGATCACCGGACCTCGAACCGTCTCCGCTTTCAAAGAGAAAGGCGTTCTCAGTGTTTCTGAGTTCGTCATCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGTATTTTCTTCTCCATTTCCATCGTCTTATTTTCTTTATTCGTTATAATTCTTGGATATTTTAATTTCGTATTATTCAACTGCTGAATTGAACGATTGTCTTTATTATTAGATTCGGTATATCAATTTTCTTTTGTGAATCTTGTCATTTTTGTTAATTCTGTTTTCAAGATAGTGGATGGAGGTCGTTTATGGTTGTTCAAAGGACATCGTGGTCAGTTGGTCTGTGTCGAACTGAAAGCCTTCCAAATTTTCAATGTAGACGATGTTGAAGGAATATAGTTAACTCCCTTTTAGTGGATGGTAGAGCTTTGGAGTCGTTGAGAAAAATCCTTAATGCCATTTCGGTCAGGAGTTCTTCAGAAACTTAAATGGAGAGTGGTTAAATAGTGATATCCCATTGGTTTTTCTTATTCTTCTGAAACTATGTTTATAGCCTTCTTTAAGAAGCAAGTTGGTTATTCGTTTTCGAAGTATAAAATTTAGTGGAACGAAATTAGAGTAGAAATCATAGTTTGTGTTTTCCAACTTACTTTTAGGCTCTTCGTGGTTGGACGGACAGTCGTAAGTCATAACGCATATGTTGGTTATCCAATATATTACTGCTTCCCATCCTATTTCTTTTTCAAGCAAAGGACAATGTGGGTTTTTCCACAATCCCATGAAAACTGACGTTATTGGTGGCTCACTTCTTCAGTTATACTTTTTATGTTCTCACCTCCTCTATTCATTTAACTCCTTAAGTTCTGGAAGTGTTTTAAAATTTGTCTATTATGTGTTAAAATATCTTTTCCTTGTTCATGATTTTAGGGAGTCGGGTGATCTGAACAAGAGGAAATCATATCTGCCCCCAGATAAGCAATTCTTGATTACCAGAAATGGTATAACAGCACAACCCTTAACTTCATTGTTTTGTATCTCTAAGCATTATTCTCGTTTCATTGCCTTAATGAAAAGTCTCGTATCCTTTTCAGAATTTTTTTTAATAAGAATTAAATGAATTTTTGGCGTAATAATCTTCAAACTTCAACTTTGTTGAGCCATTATAAAAATTAAGGTAATAGTTCACTTTCTTTCCAGTTCCTTGCCTGCGACGGGCTGCCTCTGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTCTTCTTGATAATGAAGATGGTGATGGCTGGCTGGCGACCCATGGGAAACCAAAGGGTACCTTTATCTATGAGTTTGAAGGTAGGGATTTACATTTGGTAAATAACATGTGCTCTGTCATGCAGAAGAAAAAAGTGACGAAGAGGAAAATTTGCCTTCCTTGGAAGCTCTTGAAATCAGCAGGACCAAGGTTGTTAAGTCGATTCCAAACTATTTTGGTGGTGAAGATGAAGATGATGTTCCGGACCTGGATGATATCGAAGAAACTGACAATGTTGTTGGAACTGATCCAGTATGTAAATTAAATTTTATGAGCAAAATGTGGCTTAAGAGTATTCAGTTTTACTAAATTGCCTGCATAATCACTGCTCACCCTCCTCCGCCCCAAAATTAACAAGAGTTTCAGCTATATCTATGTTTTGCAAGTTTGAGCTTTATACCATACTCTCTTAATTTGCTAGGCATAGAATGTGGAGAATAATCTGGATTTTTTCTGCATCTGATCTTGAAAAAAATTTCTTGGTTCCCATAGAGAAAGCTCTTGGTTCTTTTTTTCTTTTATCATATACACTAACAACCATTTGGAATTAGAAAAATCTTTATCCTCTCATCCAGCGGCAGTTATATTTTGATGAGAGATTCATGTTCGCATTTACTACTTCATTGTAGGCCACCCTGCCATTGACCTACCAAATTGCCCACGAACCTGATGACGATAATATCCTACGAACACGAACTTATGATGTCAGCATCACGTAAGGAGATTTCTTTTGTTGTTCTTTTTGCTGTTTTTTTACTTATGAAGAATCGTATGTAATATGTTGAATAAAAATGGATCGAAATTCATGAGTTAGGAGGAAAGAATCCTTCCATTTAGCAAACAAAAACTAGATGCAATTGAATAAATATCACTTGTAATGCAATTCGAGATATCCCACAAAAATTTTCCCATCTATGAAATCCTCCACTTTTTTTACTCCAAATCTCCAAGTACTGCAAAGAATGGATTTAGGGGTAGAGTCATAAAATATTTGCATCAGATACCCCAAATGCAGGTTACTTTCTAGTTGTTTTTCATAGTTTTTCAGGAGAAAAGATAGTTATCATTAGGAACTTTTGGTAATTATTATATGGTTCATTATTTTGATCATTGGTGCATTACTTGGAGAATCAAGCCCTTGCCATTTTTCTTGAGTAGAAAATTATCATCACTCTATCTAAAGTCTTAAGGATGAGGAAGTGTGGATTTATGGTTTAGGATTGAACTGCAGCCTATTCCATTGTTTTGTGTTCTTCCTTATCAATTTTGTGGTTATCTTATAACTGGTCCTTAGTTACAACTTTTAAGAGTTTAGGGATATAGGTAAATGTTTCTGGTTTTAAACTTTGTGGTCGTGAATTAAATCTTCAAAATTAGGCACACTGGTAGTGGTAGTGATCTTGGCTACAACCAGTGTTCCTGACTCTTAAGTCCTTTTGGTGAATCTCATTCAAAACCTACTTGATATCAAGGACTCTGGGAAATATTGTTATTAATGAAGTTTTAGCTTTCCGTGAGACATTTTCCTGTTGATTATTGTAGGTCATTAAGTTTTATCAATGGTATATGTACATGAAATAACTATTAAACAAGGGAATAGCCAGCGAAAAATAATATGCCAAAAATATCTTTGCAATAGTTAATCCTATGATACAATCTTCAAAAACTGTATCATGTCATGTTTTACCTAAAATAAGGAAGTTTGTTTATGTTCTATAATTATGCTTTTCCAGATTTCAAAAGTAGATAGTTGACTATAAGTTTGACAATGATGCTATTCTTTTCAATATCAGGTATGATAAATATTATCAGACACCACGTGTTTGGCTTACAGGATATGATGAGGTTTGTATGTTTATGTGTATATGTATTTAAATGTGATTAACTGATAACAAAATGTTAATTATAATATTTTAATTTTCACCTACACATCTTGTTAGTGGGTTGTATAGGTAGAATATTGTGTGATATTATAACTTTGAAAAAAATCTAGACAATGAATGCAATGGAGTGAAATTGATTGTTTAGTTCTTCATTGGTGAATGGTAATTGGTGTGAATATAAAGAGGATTCAACGAGTGACAGTTGTTTATCATCATTTTTCTATGTACTTTTCTGTAATGTATTTATGTCTTTCTAGTTCAAATGCATATTGCCCATTGAAATTTCTTCTTATTTCTTGATTAGTTATCCCTCCATAGTTCATGGTTTATGTTTGATTACTGCTTAATAATGTTGTTTCATAAATTATCTTTTCTTTTCAAAGCTACAGAAAATACGGCACTAACTCTACATGCTCACACTTACACCAACACTGCTTTCTATTGTGATAAAAGTTTTTTTTTCCACAATAGAATACAAATTTTTTACATGGACAACAATTTGTTATCTGATGCTTTAATTTGCAAGTTCTTTTAATGCTTTCTTACCCAATTTCTTTTAGCACATACATATTTAAATCATTGTGAACTATGACAGTCAAGGATGCTCTTACAGCCGGAACTTGTGCTTGAAGATGTTAGTCAAGATCACGCTCGAAAAACAGTAAGTTGTGAATATGTAATAATGTAGTTCTGCCATATGAGGCCCAATTATTTGTTCTATGTTATTTTCAGTTGATCTGTATGCTTAGGATTGACTTCTTCTTGTACCTTTTGCTATGCCACACTACTCAAAGAATATTAATAATCGAGAATTAGCTCTTACACCTAAATTCGAGACTTTATAAAATCTGAGAACTTGAACACAACAAATACACAAAGAAGAAAGAAAGAAAGAAAGAAAGAAGTAAAAACTTCTTAGTTAGCTAGTAACCTTTGCCTTTGCCTCCTTTATTCTTCAGCACCTAAAAATCCAGATTTACAATGACAGTAATATCTTATCCAGAAATGAAGGCTTGGCAATGAAAACATAATATTTTGATTAAACATCTCAGGTGGAGCGAGACTTTGAAAGAATATTGACACCCAATGCACAACCTGGAACCAAACATATGGTTGGGTTCGGTTATGTCCATCAACTGAATTGTTGTTTTAAGTTCAAGTTCTCAGATAGTATGGATTATGGACTTCGATCCTTGTAGATGCGACAATACATCATTTTACACCTAGATAAGAGGGCCTATTTGGATTGACTTAAGAAAAAAATTGTTTAAAATACACTTCAAAACTATTTTGAGTGGAAGGTGACTTATTTTTTAAATTAAAGACCTGAATGTATTCCAAACAAAACCAAGATTACCAACAAGAAACTGTTTTTGCTTTTGTATGCTGAACTAGTATGGGGGAGTCTGGAGAACCCATACATTTGTTTCATTGTCTACGAATTTGTCCTTACCAGTGTTACCAGTGTCATGTGGAACATGAATTTTCAAATACATTCTAAATTCAATCTTTGGGATACAGGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCTTCTGTACATCCCTGCAGACACGGGGCTGTGATGAAAAAGATCATTGACGTGTTAATGTCCCGAGGCGTAGAACCTGAAGTTCACAAGTAATTATTTCAGTCTCTATTTAAAACAACAGATCTCCCTACGATTTTGCACTTCTCGACAAATATAATCTGTTTTGTTATTCTAAGTGATTATTCTATTCTTAAGTCTTGGGTTCAAGAGTTCCTATATCACTCCAAGCGACACAATAAAAACTTTTTCTATTCTTTTATTAACTTATTATGTATAGGATTCCACTCACTCGATGGTTGGAAACTAATGATTGATTCTGTCTACAAAGATTCTGGATTTACTCATAACGATCAAATTATATCTATGCTTGTTTCTAAGTATCTCAATCTCCTTTACTTGTTAATATTTTTCCTTGTATTTTTTGTAGGTATCTTTTCTTGTTCTTAAAATTTGTTGCATCGGTTATTCCGACTATTGAATATGATTATACTATGGACTTCGATCTCGGCGGCCCTAGCAGTCAATAA

mRNA sequence

ATGGTTTTATCCCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTTGAGAGGATCACCGGACCTCGAACCGTCTCCGCTTTCAAAGAGAAAGGCGTTCTCAGTGTTTCTGAGTTCGTCATCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGGAGTCGGGTGATCTGAACAAGAGGAAATCATATCTGCCCCCAGATAAGCAATTCTTGATTACCAGAAATGTTCCTTGCCTGCGACGGGCTGCCTCTGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTCTTCTTGATAATGAAGATGGTGATGGCTGGCTGGCGACCCATGGGAAACCAAAGGAAGAAAAAAGTGACGAAGAGGAAAATTTGCCTTCCTTGGAAGCTCTTGAAATCAGCAGGACCAAGGTTGTTAAGTCGATTCCAAACTATTTTGGTGGTGAAGATGAAGATGATGTTCCGGACCTGGATGATATCGAAGAAACTGACAATGTTGTTGGAACTGATCCAGCCACCCTGCCATTGACCTACCAAATTGCCCACGAACCTGATGACGATAATATCCTACGAACACGAACTTATGATGTCAGCATCACGTATGATAAATATTATCAGACACCACGTGTTTGGCTTACAGGATATGATGAGTCAAGGATGCTCTTACAGCCGGAACTTGTGCTTGAAGATGTTAGTCAAGATCACGCTCGAAAAACAGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCTTCTGTACATCCCTGCAGACACGGGGCTGTGATGAAAAAGATCATTGACGTGTTAATGTCCCGAGGCGTAGAACCTGAAGTTCACAAGTATCTTTTCTTGTTCTTAAAATTTGTTGCATCGGTTATTCCGACTATTGAATATGATTATACTATGGACTTCGATCTCGGCGGCCCTAGCAGTCAATAA

Coding sequence (CDS)

ATGGTTTTATCCCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTTGAGAGGATCACCGGACCTCGAACCGTCTCCGCTTTCAAAGAGAAAGGCGTTCTCAGTGTTTCTGAGTTCGTCATCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGGAGTCGGGTGATCTGAACAAGAGGAAATCATATCTGCCCCCAGATAAGCAATTCTTGATTACCAGAAATGTTCCTTGCCTGCGACGGGCTGCCTCTGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTCTTCTTGATAATGAAGATGGTGATGGCTGGCTGGCGACCCATGGGAAACCAAAGGAAGAAAAAAGTGACGAAGAGGAAAATTTGCCTTCCTTGGAAGCTCTTGAAATCAGCAGGACCAAGGTTGTTAAGTCGATTCCAAACTATTTTGGTGGTGAAGATGAAGATGATGTTCCGGACCTGGATGATATCGAAGAAACTGACAATGTTGTTGGAACTGATCCAGCCACCCTGCCATTGACCTACCAAATTGCCCACGAACCTGATGACGATAATATCCTACGAACACGAACTTATGATGTCAGCATCACGTATGATAAATATTATCAGACACCACGTGTTTGGCTTACAGGATATGATGAGTCAAGGATGCTCTTACAGCCGGAACTTGTGCTTGAAGATGTTAGTCAAGATCACGCTCGAAAAACAGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCTTCTGTACATCCCTGCAGACACGGGGCTGTGATGAAAAAGATCATTGACGTGTTAATGTCCCGAGGCGTAGAACCTGAAGTTCACAAGTATCTTTTCTTGTTCTTAAAATTTGTTGCATCGGTTATTCCGACTATTGAATATGATTATACTATGGACTTCGATCTCGGCGGCCCTAGCAGTCAATAA

Protein sequence

MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKSDEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYDYTMDFDLGGPSSQ*
Homology
BLAST of Chy11G203370 vs. ExPASy Swiss-Prot
Match: Q0WWQ1 (Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2)

HSP 1 Score: 555.1 bits (1429), Expect = 5.2e-157
Identity = 265/314 (84.39%), Postives = 291/314 (92.68%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEFV+AGDNLVSKCPTWSWESGD +
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFVLAGDNLVSKCPTWSWESGDAS 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRK YLP DKQFLITRNVPCLRRAASV E+YEAAGGEVL+D+ED DGWLATHGKPK +K 
Sbjct: 61  KRKPYLPSDKQFLITRNVPCLRRAASVAEDYEAAGGEVLVDDEDNDGWLATHGKPK-DKG 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
            EE+NLPS++AL+I+    ++SIP YFGGE++DD+PD+++ +E DNVV  DPATL  TY 
Sbjct: 121 KEEDNLPSMDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDEADNVVENDPATLQSTYL 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           +AHEPDDDNILRTRTYD+SITYDKYYQTPRVWLTGYDESRMLLQPELV+EDVSQDHARKT
Sbjct: 181 VAHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLLQPELVMEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEV KYLFLFLKF+ASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIE 300

Query: 301 YDYTMDFDLGGPSS 315
           YDYTMDFDLG  S+
Sbjct: 301 YDYTMDFDLGSSST 313

BLAST of Chy11G203370 vs. ExPASy Swiss-Prot
Match: Q550A8 (Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV=3)

HSP 1 Score: 295.4 bits (755), Expect = 7.5e-79
Identity = 158/333 (47.45%), Postives = 212/333 (63.66%), Query Frame = 0

Query: 5   QKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLNKRKS 64
           Q +H+A+  TVE++T   + S F E+GVL+  EFV AGD L  KC TW+WESGD ++  S
Sbjct: 7   QAVHKAYVKTVEKVTPTLSTSKFLEEGVLTPEEFVQAGDLLTDKCQTWTWESGDPSRNVS 66

Query: 65  YLPPDKQFLITRNVPCLRRAASVEEEYEAA-GGEVLLDNEDGDGWLATHGKPKEEKSDEE 124
           YLP +KQFL+TRNVPC  R  ++E E +A+   E+ ++++  D W+A   +P   + D E
Sbjct: 67  YLPKEKQFLLTRNVPCYNRVRTLENESKASKADEIQIEDDGEDSWVAP--QPVGNQDDIE 126

Query: 125 ENLPSLEALEI----------SRTKVVKSIPNYFGGEDED---DVPDLDDIEETDNVVGT 184
           +    + +L+I          +  K   +  N    EDED   D+PDLDD ++ + +   
Sbjct: 127 DEKVDISSLKIDDKKPNTTTTTTAKPTNNNNNNNDDEDEDEDGDIPDLDDFQDDNIIEEE 186

Query: 185 DPATL-----PLTYQIAHE----------PDDDNILRTRTYDVSITYDKYYQTPRVWLTG 244
           DPA L       T   A+            D+DNILRTRTYD+SITYDKYYQTPRVWL G
Sbjct: 187 DPAVLSKNNKTTTTTTANNNNNNNSENKVEDNDNILRTRTYDISITYDKYYQTPRVWLFG 246

Query: 245 YDESRMLLQPELVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSR 304
           YDE+R  L+PE + ED+S+DHA KTVTI+ HPHL    A +HPCRH AVMKK++D     
Sbjct: 247 YDENRKPLKPEEIFEDISEDHAHKTVTIDSHPHLGISFAYIHPCRHAAVMKKLVDRQSEN 306

Query: 305 GVEPEVHKYLFLFLKFVASVIPTIEYDYTMDFD 309
           G EP V +YLFLFLKF++ VIPTIEYD+T++FD
Sbjct: 307 GKEPRVDQYLFLFLKFISVVIPTIEYDFTLEFD 337

BLAST of Chy11G203370 vs. ExPASy Swiss-Prot
Match: Q9CPX6 (Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 6.6e-67
Identity = 142/302 (47.02%), Postives = 188/302 (62.25%), Query Frame = 0

Query: 16  ERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQFLIT 75
           E +T     S FKE GV++  EFV AGD+LV  CPTW W +G+  K K+YLP DKQFL+T
Sbjct: 17  EYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTDKQFLVT 76

Query: 76  RNVPCLRRAASVEEEYEAAGGEVLLDNEDGD-GWLATHGKPKEEKSDEEENLPSLEALEI 135
           +NVPC +R   +E   E    E +++ +DGD GW+ T+         E     +LE+ + 
Sbjct: 77  KNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLESKDS 136

Query: 136 SRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQI------AHEPDDD 195
            + +   ++ +    EDE +  D+++ EE+  ++ TD ATL     +      A    +D
Sbjct: 137 IKLQDCSALCDEEDEEDEGEAADMEEYEES-GLLETDEATLDTRKIVEACKAKADAGGED 196

Query: 196 NILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKTVTIEDHPH 255
            IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  E + ED+SQDH +KTVTIE+HPH
Sbjct: 197 AILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPH 256

Query: 256 L-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYDYTMDF 310
           L P    SVHPCRH  VMKKII+ +   G E  VH YL +FLKFV +VIPTIEYDYT  F
Sbjct: 257 LPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHF 314

BLAST of Chy11G203370 vs. ExPASy Swiss-Prot
Match: Q6PFS7 (Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.3e-66
Identity = 145/307 (47.23%), Postives = 184/307 (59.93%), Query Frame = 0

Query: 13  GTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQF 72
           G  E +T     S FKE GV++  EFV AGD+LV  CPTW W SG+  K K YLP DKQF
Sbjct: 14  GVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEEAKVKPYLPNDKQF 73

Query: 73  LITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGD-GWLATHGKPKEEKSDEEENLPSLEA 132
           L+TRNVPC +R   +E   E    E +++ +DGD GW+ T          E     SL+ 
Sbjct: 74  LLTRNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTFHNSGVTGVTEAVREISLDN 133

Query: 133 LEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETD--NVVGTDPATL------PLTYQIAH 192
            +     V        G +D+D+  +  D+EE +   ++ TD ATL       L+   A 
Sbjct: 134 KDNMNMNVKTGACGNSGDDDDDEEGEAADMEEYEESGLLETDDATLDTSKMADLSKTKAE 193

Query: 193 EPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKTVTI 252
              +D IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  + + ED+SQDH +KTVTI
Sbjct: 194 AGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVDQMYEDISQDHVKKTVTI 253

Query: 253 EDHPHL-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYD 310
           E+HP+L P    SVHPCRH  VMKKII+ +   G E  VH YL +FLKFV +VIPTIEYD
Sbjct: 254 ENHPNLPPPAMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYD 313

BLAST of Chy11G203370 vs. ExPASy Swiss-Prot
Match: Q0VCL3 (Ubiquitin-like-conjugating enzyme ATG3 OS=Bos taurus OX=9913 GN=ATG3 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.1e-65
Identity = 140/302 (46.36%), Postives = 185/302 (61.26%), Query Frame = 0

Query: 16  ERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQFLIT 75
           E +T     S FKE GV++  EFV AGD+LV  CPTW W +G+  K K+YLP  KQFL+T
Sbjct: 17  EYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPSGKQFLVT 76

Query: 76  RNVPCLRRAASVEEEYEAAGGEVLLDNEDGD-GWLATHGKPKEEKSDEEENLPSLEALEI 135
           +NVPC +R   +E   E    E +++ +DGD GW+ T+         E     +LE+ + 
Sbjct: 77  KNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTYHNTGIAGITEAVKEITLESKDS 136

Query: 136 SRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQI------AHEPDDD 195
            + +   ++      EDE +  D+++ EE+  ++ TD ATL     +           +D
Sbjct: 137 IKLQDCSALCEEEEEEDEGEAADMEEYEES-GLLETDEATLDTRKIVEACKAKTDAGGED 196

Query: 196 NILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKTVTIEDHPH 255
            IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  E + ED+SQDH +KTVTIE+HPH
Sbjct: 197 AILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPH 256

Query: 256 L-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYDYTMDF 310
           L P    SVHPCRH  VMKKII+ +   G E  VH YL +FLKFV +VIPTIEYDYT  F
Sbjct: 257 LPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHF 314

BLAST of Chy11G203370 vs. ExPASy TrEMBL
Match: A0A0A0KDT1 (Autophagy_act_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G104635 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.0e-180
Identity = 313/315 (99.37%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEK 
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKR 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLE LEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 316
           YDYTMDFDLGGPSSQ
Sbjct: 301 YDYTMDFDLGGPSSQ 315

BLAST of Chy11G203370 vs. ExPASy TrEMBL
Match: A0A1S3CAI3 (autophagy-related protein 3 OS=Cucumis melo OX=3656 GN=LOC103498815 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 7.8e-180
Identity = 312/315 (99.05%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAS+EEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASIEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTD ATLPLTYQ
Sbjct: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 316
           YDYTMDFDLGGPSSQ
Sbjct: 301 YDYTMDFDLGGPSSQ 315

BLAST of Chy11G203370 vs. ExPASy TrEMBL
Match: A0A5A7T8K5 (Autophagy-related protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G00500 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 7.8e-180
Identity = 312/315 (99.05%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAS+EEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASIEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTD ATLPLTYQ
Sbjct: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 316
           YDYTMDFDLGGPSSQ
Sbjct: 301 YDYTMDFDLGGPSSQ 315

BLAST of Chy11G203370 vs. ExPASy TrEMBL
Match: A0A6J1H4I9 (autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460417 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 303/315 (96.19%), Postives = 310/315 (98.41%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGD+N
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDIN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLE LEISR KVVKSIPNYFGGEDE+D+PDL DIE+TDNV+GTD ATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIEDTDNVIGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 316
           YDYTMDFDLGGPSS+
Sbjct: 301 YDYTMDFDLGGPSSR 315

BLAST of Chy11G203370 vs. ExPASy TrEMBL
Match: A0A6J1IFS6 (autophagy-related protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111472923 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 2.6e-175
Identity = 302/315 (95.87%), Postives = 310/315 (98.41%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGD+N
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDIN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLE LEISR KVVKSIPNYFGGEDE+D+PDL DIE+TDNV+GTD ATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIEDTDNVIGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 316
           YDYTMDFDLGGP+S+
Sbjct: 301 YDYTMDFDLGGPTSR 315

BLAST of Chy11G203370 vs. NCBI nr
Match: XP_004140511.1 (autophagy-related protein 3 [Cucumis sativus] >XP_011656844.1 autophagy-related protein 3 [Cucumis sativus] >KGN46507.1 hypothetical protein Csa_005198 [Cucumis sativus])

HSP 1 Score: 634 bits (1634), Expect = 3.95e-229
Identity = 313/315 (99.37%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEK 
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKR 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLE LEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 315
           YDYTMDFDLGGPSSQ
Sbjct: 301 YDYTMDFDLGGPSSQ 315

BLAST of Chy11G203370 vs. NCBI nr
Match: XP_008459789.1 (PREDICTED: autophagy-related protein 3 [Cucumis melo] >XP_008459790.1 PREDICTED: autophagy-related protein 3 [Cucumis melo] >KAA0039782.1 autophagy-related protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 633 bits (1633), Expect = 5.61e-229
Identity = 312/315 (99.05%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAS+EEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASIEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTD ATLPLTYQ
Sbjct: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 315
           YDYTMDFDLGGPSSQ
Sbjct: 301 YDYTMDFDLGGPSSQ 315

BLAST of Chy11G203370 vs. NCBI nr
Match: XP_038876160.1 (autophagy-related protein 3-like [Benincasa hispida] >XP_038876161.1 autophagy-related protein 3-like [Benincasa hispida])

HSP 1 Score: 624 bits (1608), Expect = 3.50e-225
Identity = 309/315 (98.10%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTD ATLP TYQ
Sbjct: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDSATLP-TYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDV+ITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVTITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 315
           YDYTMDFDLGGPS++
Sbjct: 301 YDYTMDFDLGGPSTR 314

BLAST of Chy11G203370 vs. NCBI nr
Match: XP_022959442.1 (autophagy-related protein 3-like [Cucurbita moschata] >XP_022959443.1 autophagy-related protein 3-like [Cucurbita moschata] >XP_022959444.1 autophagy-related protein 3-like [Cucurbita moschata])

HSP 1 Score: 619 bits (1597), Expect = 1.73e-223
Identity = 303/315 (96.19%), Postives = 310/315 (98.41%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGD+N
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDIN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLE LEISR KVVKSIPNYFGGEDE+D+PDL DIE+TDNV+GTD ATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIEDTDNVIGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 315
           YDYTMDFDLGGPSS+
Sbjct: 301 YDYTMDFDLGGPSSR 315

BLAST of Chy11G203370 vs. NCBI nr
Match: XP_022974286.1 (autophagy-related protein 3-like [Cucurbita maxima] >XP_022974287.1 autophagy-related protein 3-like [Cucurbita maxima] >XP_022974288.1 autophagy-related protein 3-like [Cucurbita maxima])

HSP 1 Score: 618 bits (1594), Expect = 4.95e-223
Identity = 302/315 (95.87%), Postives = 310/315 (98.41%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEF+IAGDNLVSKCPTWSWESGD+N
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDIN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
           DEEENLPSLE LEISR KVVKSIPNYFGGEDE+D+PDL DIE+TDNV+GTD ATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIEDTDNVIGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDFDLGGPSSQ 315
           YDYTMDFDLGGP+S+
Sbjct: 301 YDYTMDFDLGGPTSR 315

BLAST of Chy11G203370 vs. TAIR 10
Match: AT5G61500.1 (autophagy 3 (APG3) )

HSP 1 Score: 555.1 bits (1429), Expect = 3.7e-158
Identity = 265/314 (84.39%), Postives = 291/314 (92.68%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFVIAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEFV+AGDNLVSKCPTWSWESGD +
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFVLAGDNLVSKCPTWSWESGDAS 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120
           KRK YLP DKQFLITRNVPCLRRAASV E+YEAAGGEVL+D+ED DGWLATHGKPK +K 
Sbjct: 61  KRKPYLPSDKQFLITRNVPCLRRAASVAEDYEAAGGEVLVDDEDNDGWLATHGKPK-DKG 120

Query: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDPATLPLTYQ 180
            EE+NLPS++AL+I+    ++SIP YFGGE++DD+PD+++ +E DNVV  DPATL  TY 
Sbjct: 121 KEEDNLPSMDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDEADNVVENDPATLQSTYL 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240
           +AHEPDDDNILRTRTYD+SITYDKYYQTPRVWLTGYDESRMLLQPELV+EDVSQDHARKT
Sbjct: 181 VAHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLLQPELVMEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEV KYLFLFLKF+ASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIE 300

Query: 301 YDYTMDFDLGGPSS 315
           YDYTMDFDLG  S+
Sbjct: 301 YDYTMDFDLGSSST 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WWQ15.2e-15784.39Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2[more]
Q550A87.5e-7947.45Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV... [more]
Q9CPX66.6e-6747.02Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=... [more]
Q6PFS73.3e-6647.23Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1[more]
Q0VCL32.1e-6546.36Ubiquitin-like-conjugating enzyme ATG3 OS=Bos taurus OX=9913 GN=ATG3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KDT16.0e-18099.37Autophagy_act_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G10... [more]
A0A1S3CAI37.8e-18099.05autophagy-related protein 3 OS=Cucumis melo OX=3656 GN=LOC103498815 PE=4 SV=1[more]
A0A5A7T8K57.8e-18099.05Autophagy-related protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1H4I91.2e-17596.19autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460417 P... [more]
A0A6J1IFS62.6e-17595.87autophagy-related protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111472923 PE=... [more]
Match NameE-valueIdentityDescription
XP_004140511.13.95e-22999.37autophagy-related protein 3 [Cucumis sativus] >XP_011656844.1 autophagy-related ... [more]
XP_008459789.15.61e-22999.05PREDICTED: autophagy-related protein 3 [Cucumis melo] >XP_008459790.1 PREDICTED:... [more]
XP_038876160.13.50e-22598.10autophagy-related protein 3-like [Benincasa hispida] >XP_038876161.1 autophagy-r... [more]
XP_022959442.11.73e-22396.19autophagy-related protein 3-like [Cucurbita moschata] >XP_022959443.1 autophagy-... [more]
XP_022974286.14.95e-22395.87autophagy-related protein 3-like [Cucurbita maxima] >XP_022974287.1 autophagy-re... [more]
Match NameE-valueIdentityDescription
AT5G61500.13.7e-15884.39autophagy 3 (APG3) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PFAMPF03987Autophagy_act_Ccoord: 37..303
e-value: 2.8E-66
score: 223.4
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PANTHERPTHR12866UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 1..309
NoneNo IPR availableGENE3D3.30.1460.50coord: 158..298
e-value: 3.6E-5
score: 25.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..125
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 107..125
NoneNo IPR availablePANTHERPTHR12866:SF2UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 1..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G203370.1Chy11G203370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0044804 autophagy of nucleus
cellular_component GO:0000153 cytoplasmic ubiquitin ligase complex
cellular_component GO:0005829 cytosol
molecular_function GO:0019776 Atg8 ligase activity
molecular_function GO:0019787 ubiquitin-like protein transferase activity