Chy11G201960 (gene) Cucumber (hystrix) v1

Overview


Name	Chy11G201960
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	tRNA pseudouridine synthase
Location	chrH11: 22708097 .. 22709616 (+)
RNA-Seq Expression	Chy11G201960
Synteny	Chy11G201960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGCTGTTGCTTACGACAAGTTGAGGTTTCAGGTGGCTCACTTTGTTACGCCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTACCCGATTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAGTGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACCATGCAGCCATGAAAGAAGCTGCAAATTATTTTGTCGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAATGATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGTAAGTTTTAAGGTTCTTTAATTAGTGATTTGTTTGGTTGGAATTACCAATTTAGTCTATAGACTTTGAGATTTATGTCTATTTGGTCATCTTTCAAAAGTGTTCAATAGGTCATTGCACTTCAATCTTGTGCCATATAGGACTTTGAACCTCAAAATGTATTGAAAAGTTCTTAAACTTTCAATTTCATGTCTTAGATCGCTTATTTTCGATTTTGTACTTATCCTTGACCTATTCGATAATTTCTAAAATTCACAGATTTATTGACATTAAATCGAAAGTTTAGGGTTACATTGACATAATATTCGAATTTATACATTGTAGGTCAATTAGTTTTTTATAAAAGTCGAATTTGCCAAGGACCTATTAGGCACACAATTGATAGTTTAAGGATTTATTAGACATTTTTAACTGTACGAGATCCATTTGATACATTTGAAAGTTCAAGATCAATAGATTATTAAAGTTTATGGACTAATTTAACAGTCTAACCAGCTTGTTTGTAATTTTTTTTAATCCCTCTGATGGATGTGTTTGCTTCTCAGGTCTAAAGTTGCTAATTCTGATACCCTACTTTTGATCATCTGTGTGAATCATTTCGGTTTCAGCTTTCTACAAAACCTATGAAACTAAATGCTTTCTTTTGTTCAGGGAGCTCTTGTGCAGTTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTAATTTTTGTTATATCTACCTTTACAGTCAAAAGTCCGTTCTCTGATTGTTTTATTTTTACTTAAGCTGGTTGCTAAAATGGCTTTATAGTCCTGGAAGCATAAATTTAAGTTTGTAAAATTGGTTTCATGCAACTCAGGAACTGTAAGCATTTCTCGTGTGTTTTCAGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAGAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCCCAGGGACTGTGTCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGTAGGACTCGTAGTGTGAGCAAATGTAAAGTCCCTTTTTATTGA

mRNA sequence

ATGCTTGCTGTTGCTTACGACAAGTTGAGGTTTCAGGTGGCTCACTTTGTTACGCCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTACCCGATTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAGTGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACCATGCAGCCATGAAAGAAGCTGCAAATTATTTTGTCGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAATGATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGTTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAGAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCCCAGGGACTGTGTCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGTAGGACTCGTAGTGTGAGCAAATGTAAAGTCCCTTTTTATTGA

Coding sequence (CDS)

ATGCTTGCTGTTGCTTACGACAAGTTGAGGTTTCAGGTGGCTCACTTTGTTACGCCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTACCCGATTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAGTGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACCATGCAGCCATGAAAGAAGCTGCAAATTATTTTGTCGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAATGATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGTTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAGAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCCCAGGGACTGTGTCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGTAGGACTCGTAGTGTGAGCAAATGTAAAGTCCCTTTTTATTGA

Protein sequence

MLAVAYDKLRFQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY*

Homology

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BLAST of Chy11G201960 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.8e-37
Identity = 78/196 (39.80%), Postives = 122/196 (62.24%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHF    +Y  L  +H +LNGLLP DIR++ + P  P FH+++S + K YHY ++   
Sbjct: 61  QVAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNK 120

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVV-NPVKVIFRFDV 131
           +MDPFQR Y++H   K++   +K+AA  F G HDF++F N AH      NPV+ ++R D+
Sbjct: 121 VMDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDI 180

Query: 132 IEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGV 191
                 ++LE EG GFLY+ VRN+VG L+ +       E + +I A+++RR+ +  A   
Sbjct: 181 KPNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA--- 240

Query: 192 PPQGLCLVSINYKEDH 207
           PP+GL L+ + Y+ ++
Sbjct: 241 PPEGLFLIQVFYENEN 252

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BLAST of Chy11G201960 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.0e-32
Identity = 80/193 (41.45%), Postives = 108/193 (55.96%), Query Frame = 0


Query: 10  RFQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYS 69
           R QV +FVT       E + AALNG+LP DI   +      DFHAR+S ++K Y Y +Y+
Sbjct: 60  RGQVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYN 119

Query: 70  GTIMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFD 129
             +  PFQR Y+ H    L+  AM+ AA + +G+HDFS F  A     V + V+ + R D
Sbjct: 120 HPVRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTFQAAGR--PVQSAVRTLTRAD 179

Query: 130 VIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASG 189
           +     LV+L     GFLY  VR M G L+++G E L PE +P I+AS DR   A+    
Sbjct: 180 IEAQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPT 239

Query: 190 VPPQGLCLVSINY 203
           VPP GLCL  + Y
Sbjct: 240 VPPHGLCLEEVEY 247

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BLAST of Chy11G201960 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.6e-30
Identity = 73/177 (41.24%), Postives = 102/177 (57.63%), Query Frame = 0


Query: 26  ESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQRCYTYHCV 85
           E    ALN LLP DI+V +      DFHARFS ++K Y Y IY   +M PF R Y YH  
Sbjct: 76  EKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHRVMSPFHRYYCYHEP 135

Query: 86  YKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGSG 145
            +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+IE   ++ L + G+G
Sbjct: 136 RRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADIIEDAPVINLYLRGNG 195

Query: 146 FLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCLVSINY 203
           FLY  VR + G L+ IG   + PE + KI+ S++R      A   PPQGLCL+ + Y
Sbjct: 196 FLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---PPQGLCLMEVEY 247

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BLAST of Chy11G201960 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-29
Identity = 82/192 (42.71%), Postives = 106/192 (55.21%), Query Frame = 0


Query: 11  FQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSG 70
           +QVA+F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I + 
Sbjct: 55  YQVANFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNR 114

Query: 71  TIMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDV 130
               P  R Y +H  Y L+  AMK+AA +F G HDFSAF   A  ++ VNPVK IF   V
Sbjct: 115 QFPMPTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTV 174

Query: 131 IEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGV 190
            + G L+++EVE  GFLY  VR +VG LV +G   +    +P I+ S+DR    K     
Sbjct: 175 EKEGDLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITA 234

Query: 191 PPQGLCLVSINY 203
           PPQGL LV I Y
Sbjct: 235 PPQGLYLVRIVY 240

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BLAST of Chy11G201960 vs. ExPASy Swiss-Prot
Match: Q3A3B5 (tRNA pseudouridine synthase A OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=truA PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.0e-28
Identity = 78/206 (37.86%), Postives = 111/206 (53.88%), Query Frame = 0


Query: 10  RFQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYS 69
           R  VAHFVTP     + +    +N  LP  I VR+ +    DFHAR+S + K Y Y IY 
Sbjct: 58  RGMVAHFVTPQPL-PMAAYREGVNRFLPAQIAVRDAALMPDDFHARYSAVGKWYRYSIYR 117

Query: 70  GTIMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFD 129
             +  P    +++H    L+  AM++AA   VG+HDF+AF  +    R    V+ +F  D
Sbjct: 118 SPVRSPLACRFSWHVRTVLDLQAMRQAARLLVGRHDFAAFRASGCAARTT--VREVFSVD 177

Query: 130 VIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASG 189
           +++    V ++V GSGFL   VR MVG LV++G+ A PPE V  +LA   R++     SG
Sbjct: 178 IVDAEEWVYVDVRGSGFLRNMVRIMVGTLVEVGRGARPPEEVGAMLAGGCRKQ-----SG 237

Query: 190 V--PPQGLCLVSINYKEDHLKLPSDC 214
           +  P QGLCL+ + Y       P DC
Sbjct: 238 ITAPAQGLCLMEVAY------APQDC 249

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BLAST of Chy11G201960 vs. ExPASy TrEMBL
Match: A0A1S3C7H4 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 4.6e-121
Identity = 213/221 (96.38%), Postives = 218/221 (98.64%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GT
Sbjct: 84  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 143

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           IMDPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 144 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 203

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVP
Sbjct: 204 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 263

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 233
           PQGLCLVSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 264 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

[Close]

BLAST of Chy11G201960 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 4.6e-121
Identity = 213/221 (96.38%), Postives = 218/221 (98.64%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GT
Sbjct: 114 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 173

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           IMDPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 174 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 233

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVP
Sbjct: 234 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 293

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 233
           PQGLCLVSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of Chy11G201960 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 5.9e-116
Identity = 207/208 (99.52%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT
Sbjct: 115 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 174

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           IMDPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI
Sbjct: 175 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 234

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP
Sbjct: 235 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 294

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFG 220
           PQGLCLVSINYKEDHLKLPSDCPGTSFG
Sbjct: 295 PQGLCLVSINYKEDHLKLPSDCPGTSFG 322

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BLAST of Chy11G201960 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.6e-113
Identity = 202/208 (97.12%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GT
Sbjct: 114 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 173

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           IMDPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 174 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 233

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVP
Sbjct: 234 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 293

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFG 220
           PQGLCLVSINYKEDHLKLPSDCPGTS G
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSLG 321

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BLAST of Chy11G201960 vs. ExPASy TrEMBL
Match: A0A6J1H621 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 3.6e-113
Identity = 193/221 (87.33%), Postives = 210/221 (95.02%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHF+TPFNYDSL+ +HAALNGLLP DIRVREMSPAVPDFHARFSTLSKVYHYKIY+  
Sbjct: 114 QVAHFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDA 173

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           I+DPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVI
Sbjct: 174 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVI 233

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGAL+QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDR+ELAKY + +P
Sbjct: 234 EMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIP 293

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 233
           PQGLCLV INYKEDHLKLPSDCPGTSFGR+ SVSKCK+PFY
Sbjct: 294 PQGLCLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 334

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BLAST of Chy11G201960 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 449 bits (1155), Expect = 5.59e-157
Identity = 220/221 (99.55%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT
Sbjct: 135 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 194

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           IMDPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI
Sbjct: 195 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 254

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP
Sbjct: 255 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 314

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 232
           PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY
Sbjct: 315 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

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BLAST of Chy11G201960 vs. NCBI nr
Match: XP_008458280.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo])

HSP 1 Score: 438 bits (1126), Expect = 2.39e-153
Identity = 213/221 (96.38%), Postives = 218/221 (98.64%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GT
Sbjct: 84  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 143

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           IMDPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 144 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 203

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVP
Sbjct: 204 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 263

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 232
           PQGLCLVSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 264 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

[Close]

BLAST of Chy11G201960 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 438 bits (1126), Expect = 6.92e-153
Identity = 213/221 (96.38%), Postives = 218/221 (98.64%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GT
Sbjct: 114 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 173

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           IMDPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 174 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 233

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVP
Sbjct: 234 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 293

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 232
           PQGLCLVSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of Chy11G201960 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 429 bits (1103), Expect = 2.20e-149
Identity = 206/221 (93.21%), Postives = 216/221 (97.74%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHF+TPFNYDSLESVHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G 
Sbjct: 114 QVAHFITPFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGA 173

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           I+DPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI
Sbjct: 174 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 233

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           +MG LVQLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DRRELAKYASGVP
Sbjct: 234 DMGPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVP 293

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 232
           PQGLCLVSINYKEDHLKLPSDCPGTS GR RSVSKCK+PFY
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

[Close]

BLAST of Chy11G201960 vs. NCBI nr
Match: XP_022959478.1 (uncharacterized protein LOC111460439 isoform X2 [Cucurbita moschata])

HSP 1 Score: 412 bits (1058), Expect = 1.55e-142
Identity = 193/221 (87.33%), Postives = 210/221 (95.02%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHF+TPFNYDSL+ +HAALNGLLP DIRVREMSPAVPDFHARFSTLSKVYHYKIY+  
Sbjct: 114 QVAHFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDA 173

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
           I+DPFQRCYTYHCVYKLN+AAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVI
Sbjct: 174 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVI 233

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 191
           EMGAL+QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDR+ELAKY + +P
Sbjct: 234 EMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIP 293

Query: 192 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 232
           PQGLCLV INYKEDHLKLPSDCPGTSFGR+ SVSKCK+PFY
Sbjct: 294 PQGLCLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 334

[Close]

BLAST of Chy11G201960 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 327.4 bits (838), Expect = 9.3e-90
Identity = 158/222 (71.17%), Postives = 181/222 (81.53%), Query Frame = 0


Query: 12  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 71
           QVAHFVTPFNY SL+S HAALNGLLP DIRVRE+S AVP+FHARFS  SKVY Y+IY+ T
Sbjct: 102 QVAHFVTPFNYTSLDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDT 161

Query: 72  IMDPFQRCYTYHCVYKLNHAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 131
            MDPFQR + YHC YKLN + M+EAAN FVGKHDFSAF NA   D V +P+K I RFDVI
Sbjct: 162 FMDPFQRHWAYHCAYKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVI 221

Query: 132 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYAS-GV 191
           +MG+L+QLEVEGSGFLYRQVRNMV LL+QIGKEAL  ++VP IL ++DRR LAKY S   
Sbjct: 222 QMGSLLQLEVEGSGFLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPA 281

Query: 192 PPQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 233
            P GLCLVS+ YKEDHLKLP DCP TS GR  +++KCK+PFY
Sbjct: 282 SPHGLCLVSVKYKEDHLKLPLDCPVTSSGRHHTITKCKLPFY 323

[Close]

BLAST of Chy11G201960 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.9e-10
Identity = 54/167 (32.34%), Postives = 74/167 (44.31%), Query Frame = 0


Query: 32  LNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQRCYTYHCVYKLNHA 91
           LN  LP DIRV   SPA  DFHARFS  ++ Y Y  +                   LN +
Sbjct: 191 LNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWR----------------QNLNLS 250

Query: 92  AMKEAANYFVGKHDFSAF-----TNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGSGF 151
           AM  A   F+G+HDF  F      N     R V   +V    +  E   L    + GS F
Sbjct: 251 AMDFAGKKFIGEHDFRNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAF 310

Query: 152 LYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKY--ASGVP 192
           L+ Q+R MV +L  IG+     +V+  +L ++      +Y  AS +P
Sbjct: 311 LWHQIRCMVAVLFMIGQGVESVDVIDTLLDTKKTPRKPQYLLASEIP 341

The following BLAST results are available for this feature:

BLAST of Chy11G201960 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6MEE7	1.8e-37	39.80	tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=...	[more]
B1I1B5	1.0e-32	41.45	tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477...	[more]
A4J151	3.6e-30	41.24	tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491...	[more]
Q8R7Y7	2.4e-29	42.71	tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongen...	[more]
Q3A3B5	2.0e-28	37.86	tRNA pseudouridine synthase A OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC...	[more]

BLAST of Chy11G201960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7H4	4.6e-121	96.38	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A1S3C7L6	4.6e-121	96.38	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A0A0KB67	5.9e-116	99.52	tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1	[more]
A0A5D3BY40	1.6e-113	97.12	Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1H621	3.6e-113	87.33	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]

BLAST of Chy11G201960 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138518.2	5.59e-157	99.55	uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical ...	[more]
XP_008458280.1	2.39e-153	96.38	PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo]	[more]
XP_008458279.1	6.92e-153	96.38	PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo]	[more]
XP_038875516.1	2.20e-149	93.21	tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida]	[more]
XP_022959478.1	1.55e-142	87.33	uncharacterized protein LOC111460439 isoform X2 [Cucurbita moschata]	[more]

BLAST of Chy11G201960 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06950.1	9.3e-90	71.17	Pseudouridine synthase family protein	[more]
AT1G34150.1	1.9e-10	32.34	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 59..201 e-value: 1.4E-39 score: 137.1
IPR001406	Pseudouridine synthase I, TruA	PIRSF	PIRSF001430	PSU-A	coord: 10..217 e-value: 6.4E-53 score: 177.6
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 12..215
IPR001406	Pseudouridine synthase I, TruA	CDD	cd02570	PseudoU_synth_EcTruA	coord: 12..202 e-value: 2.4804E-76 score: 228.512
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 96..202 e-value: 2.1E-25 score: 89.3
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 9..58 e-value: 2.2E-5 score: 26.7
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 12..215
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 11..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy11G201960.1	Chy11G201960.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034196	acylglycerol transport
biological_process	GO:1990052	ER to chloroplast lipid transport
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0008033	tRNA processing
biological_process	GO:0009451	RNA modification
molecular_function	GO:0070300	phosphatidic acid binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity