Chy11G201480 (gene) Cucumber (hystrix) v1

Overview


Name	Chy11G201480
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pectinesterase inhibitor-like
Location	chrH11: 22190905 .. 22192990 (+)
RNA-Seq Expression	Chy11G201480
Synteny	Chy11G201480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCAATTCCAATTTGAATTTTGGAGTCTATAGCTCTATTACTGTTTTTCTATTTGGTCTTCTTTTGTCCAATAATGTCATTCCTTTGAATGCTGATACGATACTATCTAACATCGACACTTTAGCATCGAATATATGTCCACAAACCAGTAATCCATCGTTTTGTGCAAGCGTATTGAAAAATGCAAACAACATAGATTTGAAAGCTTTGATCACTTACAACCTAAAACTTGCTCATACAAATGCAGGAAAATCTATGACTCTGGCCAAAGCACTTGCAGTATTGACAACCAATACTCATCTTAAGAAACGATATTTGTTTTGCTTTGAGAATTACGACGAGGCGATGTGTGACATGGAAAAAGCTAAAAATGATTTGGCATTTGGTGACTATAGCGGTGTTAATCTTGCTATTTCTGATGCAATGATGATAGCTGATGACTGTCATGATAACTTCGAACAACCGTTGAAAGATATGTCATTGCTTCCAAATAATACCAAGATGTTGAAAGACATTTATAGCATTATTTTAGTTATATCAAGTATTCTACCTAAGAATATATAATGAGAGAAACAACTCATGTAAGCTCTCATTAATGTTCGTTAATAAATCTGCTCTCATATTATTCATCAAATGCATGCAAGAGAAATAGTTATTATTATTATTATTTTTAAATCATGTAAAAAGGGAATAGAGATTTACTTTAATTTGGAGAACTTGATGTGGGAAAGAGTGATTATTAATGTTCTAATTTAAAGATCATTCGAGACTAGTTAGGCATGTTTCTGACTACTAAAGATGTACGTGGTGGTGGTGATACGTTATATTTAAACTTTTTAAAGTAGCTTCTTTTAAATATTTAAAAGTGATTTTGAATTATTACATGGTTGGTTGTAAAATTATTTTAATATTACTAAATTACTAGAAAGATATTTTATATTTGATCAAATTATTTTAAATTAGTTATTACCATAAATCTAATTTAATTAATATATTTTTAACATGGAATAATTTTACGGCTAAAATTTCTTTTCTAAATTTGTAATAATATTTTTGTTTCACTGTTTTACCGAATCACCAAAAGAAAAATCACTTTCTTAAAGTTTTTTTTCTTCTTTCAAAAACCCATTTTATTTTCTCACCAAACATATAGACGTTAGGGGAAAGTGATTTTGACAGTTATAGAAATGATTTTAGATTTTATATATAATTATATGGATGGCAATAAGTAGCATGACTTATTCTACAAATTGAATGTTTGGTTTTGACATCCAATGATTATTATATACTGAAAAAAGGGTTTCAAAGAGTAAAACAAACTGAATTTTAATTATAATTTTAAATGTTATCCTATAAAGTCTCCCATCCAAATGAAAAATTCTGTCTCCTCCATTGATTTCCTTTAAATTCGGAGAGAAAAAAGAAAGAAAAATTTTCTGTAAATTTCTTCTATATATATATACATATTGAAATGATCCATATCAACAATGAAAAAAGAAAAAGAGAAAGAAAGAAAAAAAGATATAAAAAAATGACCAATCTCAATCTCAATGTTCGAATCTTTGGCTCGGTTGCCTTAGTTTTCGTTTTTTCTTTTCTTTTCTTCAGCGTTGTTCCTTCTCGTGCTAACACAACGACACCATCAAATGACGTAGCATCGAGCATATGTTCGAAAACTAGAAATCCACCATTTTGCGTGGACGTGTTGAAATCTGCAGGCAGCACAGACCTGAAAGTGTTAGCCACTTACACCCTCAACCTTGCACATAAAAATGCAGTAAAATCTACAAATTTAGCCAAGTCGCTTGCAGCAATGACAACCAATCCTCCGCTTAAGAATCAATATTTGTCTTGCTACGAGAGCTACAAGGAGGCCATGAGCGACATCGAAAATGCTAAAAGCAATTTGGCCTCCGGTGATTTCAATGGTGTCAACATTGCAACTTCTGGTGTCATGACGTCGGCCAACGACTGTCAAGATAGCTTCAAACAATCACGGATAGATCCATCACTGCTCAAGGATGGAAAGACTTTAAACGATGTATGTAGCATTATTTTAGTTATATCTAATCTTCTTCCATAG

mRNA sequence

ATGATCAATTCCAATTTGAATTTTGGAGTCTATAGCTCTATTACTGTTTTTCTATTTGGTCTTCTTTTGTCCAATAATGTCATTCCTTTGAATGCTGATACGATACTATCTAACATCGACACTTTAGCATCGAATATATGTCCACAAACCAGTAATCCATCGTTTTGTGCAAGCGTATTGAAAAATGCAAACAACATAGATTTGAAAGCTTTGATCACTTACAACCTAAAACTTGCTCATACAAATGCAGGAAAATCTATGACTCTGGCCAAAGCACTTGCAGTATTGACAACCAATACTCATCTTAAGAAACGATATTTGTTTTGCTTTGAGAATTACGACGAGGCGATGTGTGACATGGAAAAAGCTAAAAATGATTTGGCATTTGGTGACTATAGCGGTGTTAATCTTGCTATTTCTGATGCAATGATGATAGCTGATGACTGTCATGATAACTTCGAACAACCGTTGAAAGATATGTCATTGCTTCCAAATAATACCAAGATGTTGAAAGACATTTATAGCATTATTTTAGTTATATCAAGCAGCACAGACCTGAAAGTGTTAGCCACTTACACCCTCAACCTTGCACATAAAAATGCAGTAAAATCTACAAATTTAGCCAAGTCGCTTGCAGCAATGACAACCAATCCTCCGCTTAAGAATCAATATTTGTCTTGCTACGAGAGCTACAAGGAGGCCATGAGCGACATCGAAAATGCTAAAAGCAATTTGGCCTCCGGTGATTTCAATGGTGTCAACATTGCAACTTCTGGTGTCATGACGTCGGCCAACGACTGTCAAGATAGCTTCAAACAATCACGGATAGATCCATCACTGCTCAAGGATGGAAAGACTTTAAACGATGTATGTAGCATTATTTTAGTTATATCTAATCTTCTTCCATAG

Coding sequence (CDS)

ATGATCAATTCCAATTTGAATTTTGGAGTCTATAGCTCTATTACTGTTTTTCTATTTGGTCTTCTTTTGTCCAATAATGTCATTCCTTTGAATGCTGATACGATACTATCTAACATCGACACTTTAGCATCGAATATATGTCCACAAACCAGTAATCCATCGTTTTGTGCAAGCGTATTGAAAAATGCAAACAACATAGATTTGAAAGCTTTGATCACTTACAACCTAAAACTTGCTCATACAAATGCAGGAAAATCTATGACTCTGGCCAAAGCACTTGCAGTATTGACAACCAATACTCATCTTAAGAAACGATATTTGTTTTGCTTTGAGAATTACGACGAGGCGATGTGTGACATGGAAAAAGCTAAAAATGATTTGGCATTTGGTGACTATAGCGGTGTTAATCTTGCTATTTCTGATGCAATGATGATAGCTGATGACTGTCATGATAACTTCGAACAACCGTTGAAAGATATGTCATTGCTTCCAAATAATACCAAGATGTTGAAAGACATTTATAGCATTATTTTAGTTATATCAAGCAGCACAGACCTGAAAGTGTTAGCCACTTACACCCTCAACCTTGCACATAAAAATGCAGTAAAATCTACAAATTTAGCCAAGTCGCTTGCAGCAATGACAACCAATCCTCCGCTTAAGAATCAATATTTGTCTTGCTACGAGAGCTACAAGGAGGCCATGAGCGACATCGAAAATGCTAAAAGCAATTTGGCCTCCGGTGATTTCAATGGTGTCAACATTGCAACTTCTGGTGTCATGACGTCGGCCAACGACTGTCAAGATAGCTTCAAACAATCACGGATAGATCCATCACTGCTCAAGGATGGAAAGACTTTAAACGATGTATGTAGCATTATTTTAGTTATATCTAATCTTCTTCCATAG

Protein sequence

MINSNLNFGVYSSITVFLFGLLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASVLKNANNIDLKALITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDMEKAKNDLAFGDYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVISSSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIENAKSNLASGDFNGVNIATSGVMTSANDCQDSFKQSRIDPSLLKDGKTLNDVCSIILVISNLLP*

Homology

[Close]

BLAST of Chy11G201480 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 45/121 (37.19%), Postives = 68/121 (56.20%), Query Frame = 0


Query: 181 SSSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIEN 240
           +S  DL+ LA  T   A  +A +     +SL    TNP +K  Y SC + YK A+S + +
Sbjct: 53  TSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLND 112

Query: 241 AKSNLASGDFNGVNIATSGVMTSANDCQDSFKQSRIDPSLLKDGKTLNDVCSIILVISNL 300
           AK +LASGD  G+NI  S  M   + C+      ++DPS +K+     ++C I+LVISN+
Sbjct: 113 AKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSAVKNSGDFQNICGIVLVISNM 172

Query: 301 L 302
           +
Sbjct: 173 M 173

[Close]

BLAST of Chy11G201480 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 2.1e-14
Identity = 58/175 (33.14%), Postives = 94/175 (53.71%), Query Frame = 0


Query: 11  YSSITVFLFGLLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASVLKN---ANNID 70
           Y S ++F+  LL+   + PL+    +   + L S ICP+T NPS C   L++   + + D
Sbjct: 5   YCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKD 64

Query: 71  LKALITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDMEKAKNDL 130
           LK L  +++ +A  +A ++  +  +L    T+  LK RY  C ENY +A+  + +AK  L
Sbjct: 65  LKGLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFL 124

Query: 131 AFGDYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVISS 183
             GDY+ +N+  S A   A  C D+FE P    + L      L+D+  I+LVIS+
Sbjct: 125 TSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISN 179

[Close]

BLAST of Chy11G201480 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.6e-12
Identity = 43/122 (35.25%), Postives = 71/122 (58.20%), Query Frame = 0


Query: 182 SSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIENA 241
           +S +L+ LA  TL+     A ++    +S+     +P  K  Y SC + Y+ A+ ++E A
Sbjct: 53  ASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEA 112

Query: 242 KSNLASGDFNGVNIATSGVMTSANDCQDSFKQSR-IDPSLLKDGKTLNDVCSIILVISNL 301
             +LASGD  G+N+  S  +  A+ C D  K+ R +D S++ + KT+ ++C I LVISN+
Sbjct: 113 FEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNM 172

Query: 302 LP 303
           LP
Sbjct: 173 LP 174

[Close]

BLAST of Chy11G201480 vs. ExPASy TrEMBL
Match: A0A0A0KCC4 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307360 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 5.5e-82
Identity = 164/182 (90.11%), Postives = 172/182 (94.51%), Query Frame = 0


Query: 1   MINSNLNFGVYSSITVFLFGLLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASVL 60
           MI+SNLNFG      VFLFG LLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCAS+L
Sbjct: 1   MISSNLNFG------VFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASIL 60

Query: 61  KNANNIDLKALITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDM 120
           +NANNIDLKALI Y+LKLAHTNAGKSMTLAKALAVLTTNT LKK+YLFCFENYDEAMCD+
Sbjct: 61  ENANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDI 120

Query: 121 EKAKNDLAFGDYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVI 180
           EKAKNDL FGDYSGVNLAISDAMMIADDCHD+F+QPLKDMSLLPNNTKMLKDIYSIILVI
Sbjct: 121 EKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVI 176

Query: 181 SS 183
           SS
Sbjct: 181 SS 176

[Close]

BLAST of Chy11G201480 vs. ExPASy TrEMBL
Match: A0A5D3BVW2 (Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00340 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 9.1e-77
Identity = 157/183 (85.79%), Postives = 166/183 (90.71%), Query Frame = 0


Query: 1   MINSNLNFGVYSSITVFLFGLLLSNN-VIPLNADTILSNIDTLASNICPQTSNPSFCASV 60
           MINSNLN GVYSSI VFLFGLLLSN   IPLNA+TI SN D L SNICPQTSNPSFCAS+
Sbjct: 1   MINSNLNLGVYSSIMVFLFGLLLSNTYAIPLNANTIPSNGDILVSNICPQTSNPSFCASI 60

Query: 61  LKNANNIDLKALITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCD 120
           L++ANNIDLKALI Y L LAHTNAGKSMTLAKALAVLTTN  LKK+YL CFENYDEAMCD
Sbjct: 61  LESANNIDLKALIAYGLNLAHTNAGKSMTLAKALAVLTTNPRLKKQYLSCFENYDEAMCD 120

Query: 121 MEKAKNDLAFGDYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILV 180
           +EKAK+DLAFGDY+GVNLAISDAMMIADDCHDNFEQ LKDMSLLPNNT+ LKDIYSIILV
Sbjct: 121 IEKAKSDLAFGDYNGVNLAISDAMMIADDCHDNFEQSLKDMSLLPNNTRTLKDIYSIILV 180

Query: 181 ISS 183
           ISS
Sbjct: 181 ISS 183

[Close]

BLAST of Chy11G201480 vs. ExPASy TrEMBL
Match: A0A1S3C9N7 (pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103498014 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 8.3e-70
Identity = 144/168 (85.71%), Postives = 153/168 (91.07%), Query Frame = 0


Query: 16  VFLFGLLLSNN-VIPLNADTILSNIDTLASNICPQTSNPSFCASVLKNANNIDLKALITY 75
           VFLFGLLLSN   IPLNA+TI SN D L SNICPQTSNPSFCAS+L++ANNIDLKALI Y
Sbjct: 2   VFLFGLLLSNTYAIPLNANTIPSNGDILVSNICPQTSNPSFCASILESANNIDLKALIAY 61

Query: 76  NLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDMEKAKNDLAFGDYSG 135
            L LAHTNAGKSMTLAKALAVLTTN  LKK+YL CFENYDEAMCD+EKAK+DLAFGDY+G
Sbjct: 62  GLNLAHTNAGKSMTLAKALAVLTTNPRLKKQYLSCFENYDEAMCDIEKAKSDLAFGDYNG 121

Query: 136 VNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVISS 183
           VNLAISDAMMIADDCHDNFEQ LKDMSLLPNNT+ LKDIYSIILVISS
Sbjct: 122 VNLAISDAMMIADDCHDNFEQSLKDMSLLPNNTRTLKDIYSIILVISS 169

[Close]

BLAST of Chy11G201480 vs. ExPASy TrEMBL
Match: A0A0A0KEW6 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307350 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 7.8e-52
Identity = 112/126 (88.89%), Postives = 119/126 (94.44%), Query Frame = 0


Query: 177 ILVISSSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMS 236
           +L  + STDLKVLATYTLNLA++NA+KSTNLAKSLAAMTTNPPLKNQYLSCYESY+EA S
Sbjct: 57  VLKSAGSTDLKVLATYTLNLANENALKSTNLAKSLAAMTTNPPLKNQYLSCYESYEEATS 116

Query: 237 DIENAKSNLASGDFNGVNIATSGVMTSANDCQDSFKQSRIDPSLLKDGKTLNDVCSIILV 296
           DIENAKSNLASGDFNGVNIATSGVMTS +DC DSFKQ RIDPSLLKDGKTLNDVCSIILV
Sbjct: 117 DIENAKSNLASGDFNGVNIATSGVMTSVSDCLDSFKQLRIDPSLLKDGKTLNDVCSIILV 176

Query: 297 ISNLLP 303
           ISNLLP
Sbjct: 177 ISNLLP 182

[Close]

BLAST of Chy11G201480 vs. ExPASy TrEMBL
Match: A0A1S3C2V0 (pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103496391 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.7e-46
Identity = 123/261 (47.13%), Postives = 148/261 (56.70%), Query Frame = 0


Query: 14  ITVFLFGLLLSNNVIPLNADTILSNI---DTLASNICPQTSNPSFCASVLKNANNIDLKA 73
           I V L G+LL          TI+SN    + + S ICP+TSN  FC+SVLK+A   DLK 
Sbjct: 8   IIVSLIGVLLF---------TIISNATSSNNVVSTICPKTSNLPFCSSVLKSAGTTDLKG 67

Query: 74  LITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDMEKAKNDLAFG 133
           L  Y L LAHTNA KS+TLA +LA  TTN  LK+RY  C ENYDEA+ D+E A+ +LA G
Sbjct: 68  LAIYTLNLAHTNARKSLTLANSLAKSTTNPQLKQRYFSCAENYDEAVGDIENAQKNLALG 127

Query: 134 DYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVISSSTDLKVLA 193
           D++ VN+  S AM   +DC D   QP K+  LL  N K + DI +IIL            
Sbjct: 128 DFNAVNIVTSGAMTEINDCQDKLAQPSKNTLLLLKNGKTINDICNIIL------------ 187

Query: 194 TYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIENAKSNLASGDF 253
                        S  L  SL    TNP LK  Y SC ESY EA+ D ENA+ +LA GDF
Sbjct: 188 -------------SLTLLNSLVKTITNPQLKQCYSSCAESYDEAVGDTENAQKDLALGDF 234

Query: 254 NGVNIATSGVMTSANDCQDSF 272
           N VNI TSG MT  +DC D F
Sbjct: 248 NAVNIVTSGAMTEIDDCHDKF 234

[Close]

BLAST of Chy11G201480 vs. NCBI nr
Match: KAE8647075.1 (hypothetical protein Csa_022944 [Cucumis sativus])

HSP 1 Score: 499 bits (1285), Expect = 7.84e-174
Identity = 262/289 (90.66%), Postives = 275/289 (95.16%), Query Frame = 0


Query: 1   MINSNLNFGVYSSITVFLFGLLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASVL 60
           MI+SNLNFGV      FLFG LLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCAS+L
Sbjct: 1   MISSNLNFGV------FLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASIL 60

Query: 61  KNANNIDLKALITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDM 120
           +NANNIDLKALI Y+LKLAHTNAGKSMTLAKALAVLTTNT LKK+YLFCFENYDEAMCD+
Sbjct: 61  ENANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDI 120

Query: 121 EKAKNDLAFGDYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVI 180
           EKAKNDL FGDYSGVNLAISDAMMIADDCHD+F+QPLKDMSLLPNNTKMLKDIYSIILVI
Sbjct: 121 EKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVI 180

Query: 181 SSSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIEN 240
           SSSTDLKVLATYTLNLA++NA+KSTNLAKSLAAMTTNPPLKNQYLSCYESY+EA SDIEN
Sbjct: 181 SSSTDLKVLATYTLNLANENALKSTNLAKSLAAMTTNPPLKNQYLSCYESYEEATSDIEN 240

Query: 241 AKSNLASGDFNGVNIATSGVMTSANDCQDSFKQSRIDPSLLKDGKTLND 289
           AKSNLASGDFNGVNIATSGVMTS +DC DSFKQ RIDPSLLKDGKTLND
Sbjct: 241 AKSNLASGDFNGVNIATSGVMTSVSDCLDSFKQLRIDPSLLKDGKTLND 283

[Close]

BLAST of Chy11G201480 vs. NCBI nr
Match: XP_031742367.1 (pectinesterase inhibitor-like [Cucumis sativus])

HSP 1 Score: 313 bits (802), Expect = 9.92e-105
Identity = 164/182 (90.11%), Postives = 172/182 (94.51%), Query Frame = 0


Query: 1   MINSNLNFGVYSSITVFLFGLLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASVL 60
           MI+SNLNFGV      FLFG LLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCAS+L
Sbjct: 1   MISSNLNFGV------FLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASIL 60

Query: 61  KNANNIDLKALITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDM 120
           +NANNIDLKALI Y+LKLAHTNAGKSMTLAKALAVLTTNT LKK+YLFCFENYDEAMCD+
Sbjct: 61  ENANNIDLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDI 120

Query: 121 EKAKNDLAFGDYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVI 180
           EKAKNDL FGDYSGVNLAISDAMMIADDCHD+F+QPLKDMSLLPNNTKMLKDIYSIILVI
Sbjct: 121 EKAKNDLVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVI 176

Query: 181 SS 182
           SS
Sbjct: 181 SS 176

[Close]

BLAST of Chy11G201480 vs. NCBI nr
Match: TYK02952.1 (pectinesterase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 295 bits (756), Expect = 1.24e-97
Identity = 157/183 (85.79%), Postives = 166/183 (90.71%), Query Frame = 0


Query: 1   MINSNLNFGVYSSITVFLFGLLLSNN-VIPLNADTILSNIDTLASNICPQTSNPSFCASV 60
           MINSNLN GVYSSI VFLFGLLLSN   IPLNA+TI SN D L SNICPQTSNPSFCAS+
Sbjct: 1   MINSNLNLGVYSSIMVFLFGLLLSNTYAIPLNANTIPSNGDILVSNICPQTSNPSFCASI 60

Query: 61  LKNANNIDLKALITYNLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCD 120
           L++ANNIDLKALI Y L LAHTNAGKSMTLAKALAVLTTN  LKK+YL CFENYDEAMCD
Sbjct: 61  LESANNIDLKALIAYGLNLAHTNAGKSMTLAKALAVLTTNPRLKKQYLSCFENYDEAMCD 120

Query: 121 MEKAKNDLAFGDYSGVNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILV 180
           +EKAK+DLAFGDY+GVNLAISDAMMIADDCHDNFEQ LKDMSLLPNNT+ LKDIYSIILV
Sbjct: 121 IEKAKSDLAFGDYNGVNLAISDAMMIADDCHDNFEQSLKDMSLLPNNTRTLKDIYSIILV 180

Query: 181 ISS 182
           ISS
Sbjct: 181 ISS 183

[Close]

BLAST of Chy11G201480 vs. NCBI nr
Match: XP_008458685.1 (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 273 bits (697), Expect = 6.95e-89
Identity = 144/168 (85.71%), Postives = 153/168 (91.07%), Query Frame = 0


Query: 16  VFLFGLLLSNN-VIPLNADTILSNIDTLASNICPQTSNPSFCASVLKNANNIDLKALITY 75
           VFLFGLLLSN   IPLNA+TI SN D L SNICPQTSNPSFCAS+L++ANNIDLKALI Y
Sbjct: 2   VFLFGLLLSNTYAIPLNANTIPSNGDILVSNICPQTSNPSFCASILESANNIDLKALIAY 61

Query: 76  NLKLAHTNAGKSMTLAKALAVLTTNTHLKKRYLFCFENYDEAMCDMEKAKNDLAFGDYSG 135
            L LAHTNAGKSMTLAKALAVLTTN  LKK+YL CFENYDEAMCD+EKAK+DLAFGDY+G
Sbjct: 62  GLNLAHTNAGKSMTLAKALAVLTTNPRLKKQYLSCFENYDEAMCDIEKAKSDLAFGDYNG 121

Query: 136 VNLAISDAMMIADDCHDNFEQPLKDMSLLPNNTKMLKDIYSIILVISS 182
           VNLAISDAMMIADDCHDNFEQ LKDMSLLPNNT+ LKDIYSIILVISS
Sbjct: 122 VNLAISDAMMIADDCHDNFEQSLKDMSLLPNNTRTLKDIYSIILVISS 169

[Close]

BLAST of Chy11G201480 vs. NCBI nr
Match: XP_031742871.1 (pectinesterase inhibitor [Cucumis sativus])

HSP 1 Score: 213 bits (542), Expect = 2.66e-65
Identity = 111/122 (90.98%), Postives = 117/122 (95.90%), Query Frame = 0


Query: 181 SSSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIEN 240
           + STDLKVLATYTLNLA++NA+KSTNLAKSLAAMTTNPPLKNQYLSCYESY+EA SDIEN
Sbjct: 61  AGSTDLKVLATYTLNLANENALKSTNLAKSLAAMTTNPPLKNQYLSCYESYEEATSDIEN 120

Query: 241 AKSNLASGDFNGVNIATSGVMTSANDCQDSFKQSRIDPSLLKDGKTLNDVCSIILVISNL 300
           AKSNLASGDFNGVNIATSGVMTS +DC DSFKQ RIDPSLLKDGKTLNDVCSIILVISNL
Sbjct: 121 AKSNLASGDFNGVNIATSGVMTSVSDCLDSFKQLRIDPSLLKDGKTLNDVCSIILVISNL 180

Query: 301 LP 302
           LP
Sbjct: 181 LP 182

[Close]

BLAST of Chy11G201480 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 88.6 bits (218), Expect = 9.5e-18
Identity = 45/121 (37.19%), Postives = 68/121 (56.20%), Query Frame = 0


Query: 181 SSSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIEN 240
           +S  DL+ LA  T   A  +A +     +SL    TNP +K  Y SC + YK A+S + +
Sbjct: 53  TSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLND 112

Query: 241 AKSNLASGDFNGVNIATSGVMTSANDCQDSFKQSRIDPSLLKDGKTLNDVCSIILVISNL 300
           AK +LASGD  G+NI  S  M   + C+      ++DPS +K+     ++C I+LVISN+
Sbjct: 113 AKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSAVKNSGDFQNICGIVLVISNM 172

Query: 301 L 302
           +
Sbjct: 173 M 173

[Close]

BLAST of Chy11G201480 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-13
Identity = 43/122 (35.25%), Postives = 71/122 (58.20%), Query Frame = 0


Query: 182 SSTDLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIENA 241
           +S +L+ LA  TL+     A ++    +S+     +P  K  Y SC + Y+ A+ ++E A
Sbjct: 53  ASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEA 112

Query: 242 KSNLASGDFNGVNIATSGVMTSANDCQDSFKQSR-IDPSLLKDGKTLNDVCSIILVISNL 301
             +LASGD  G+N+  S  +  A+ C D  K+ R +D S++ + KT+ ++C I LVISN+
Sbjct: 113 FEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNM 172

Query: 302 LP 303
           LP
Sbjct: 173 LP 174

[Close]

BLAST of Chy11G201480 vs. TAIR 10
Match: AT1G48010.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 5.2e-08
Identity = 40/123 (32.52%), Postives = 65/123 (52.85%), Query Frame = 0


Query: 185 DLKVLATYTLNLAHKNAVKSTNLAKSLAAMTTNPPLKNQYLSCYESYKEAMSDIENAKSN 244
           DL  +A   ++ A +NA+ + N  + LA  TT+   K  Y+SC + Y +A+   + A  +
Sbjct: 57  DLPGVAKCLIHYAQQNALDTHNQLQLLANSTTDNRTKESYISCSKYYDKALYSFDEALKD 116

Query: 245 LASGDFNGVNIATSGVMTSANDC-----QDSFKQSRIDPSLLKDGKTLNDVCSIILVISN 303
           L   + + +NI  +    +ANDC     +D FK    DP L+     L +VC I+L IS+
Sbjct: 117 LKVNNPDYLNIEITAARQNANDCKTLDLKDVFKP---DPKLIMKIDFLENVCGILLSISD 176

The following BLAST results are available for this feature:

BLAST of Chy11G201480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LUV1	1.3e-16	37.19	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
P83326	2.1e-14	33.14	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	2.6e-12	35.25	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]

BLAST of Chy11G201480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCC4	5.5e-82	90.11	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307360 PE=4 S...	[more]
A0A5D3BVW2	9.1e-77	85.79	Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3C9N7	8.3e-70	85.71	pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103498014 PE=4 SV=1	[more]
A0A0A0KEW6	7.8e-52	88.89	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307350 PE=4 S...	[more]
A0A1S3C2V0	1.7e-46	47.13	pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103496391 PE=4 SV=1	[more]

BLAST of Chy11G201480 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647075.1	7.84e-174	90.66	hypothetical protein Csa_022944 [Cucumis sativus]	[more]
XP_031742367.1	9.92e-105	90.11	pectinesterase inhibitor-like [Cucumis sativus]	[more]
TYK02952.1	1.24e-97	85.79	pectinesterase inhibitor-like [Cucumis melo var. makuwa]	[more]
XP_008458685.1	6.95e-89	85.71	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]
XP_031742871.1	2.66e-65	90.98	pectinesterase inhibitor [Cucumis sativus]	[more]

BLAST of Chy11G201480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17220.1	9.5e-18	37.19	pectin methylesterase inhibitor 2	[more]
AT1G48020.1	1.8e-13	35.25	pectin methylesterase inhibitor 1	[more]
AT1G48010.1	5.2e-08	32.52	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 38..180 e-value: 2.3E-15 score: 67.1 coord: 181..297 e-value: 3.6E-9 score: 46.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 43..179 e-value: 9.7E-12 score: 45.4 coord: 183..297 e-value: 5.2E-10 score: 39.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 182..300 e-value: 3.7E-17 score: 60.2 coord: 11..181 e-value: 3.0E-22 score: 76.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 39..181 e-value: 2.5E-22 score: 81.5 coord: 182..301 e-value: 1.2E-22 score: 82.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 178..301
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 44..182
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 14..182 coord: 182..301
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 182..301 coord: 14..182
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 44..182 e-value: 6.37169E-27 score: 100.96
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 181..301 e-value: 3.35478E-27 score: 101.73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy11G201480.1	Chy11G201480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity