Chy11G200120 (gene) Cucumber (hystrix) v1

Overview
NameChy11G200120
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionadenylate isopentenyltransferase 5, chloroplastic-like
LocationchrH11: 21097821 .. 21098801 (+)
RNA-Seq ExpressionChy11G200120
SyntenyChy11G200120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATTTCGTTATCGACAAGCTGCAAGCAAGTTGCGGTGGAGCCGCCGCTGGTAAACTTTCAAGGCACAAACAACACACTGAATTTGGAACCATTTTTCCGGCGAAAAGAGAAAGTGGTATTTGTAATGGGGGCTACCGGCACTGGGAAATCACGGTTGGCAATTGATTTAGCCACTCGTTTCCCAGCTGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCAAAGTCCTCACCAATAAAGTCACTGAAAAAGAATGTCATGGCGTCCCACATCACCTCCTTGGAATCGCTGATCCCAATTCAAACTTCACTGTTGCCGACTTCCGCCGCCATGCCTCATCGGCGATCAACTCCATTATTATAGGAAAACGTCGTCTCCCTATTCTGGCCGGCGGCTCCAACTCCTTCATCGAATCACTTGTAGAGGAGGAGCCTGAGTTTCGTTACCGTTTTGATTGTTGCTTCTTATGGGTGGATGTTTCGTTACCCATCCTCAACTCGTTTGTGTCGGAACGTGTTGATCGGATGTTGGAAAATGGATTAGTTAATGAGGTACGGAATTTATTTGATCCTAATGGATTAGGAAATGATTATGGACGTGGAATTCGACGCGCAATTGGAGTTCCGGAACTTGACGCATTTCTACATGCTGAGATGGATTCTGCCACTGATGATAAAGCTCGATCTCGAATTCTTAAGGCTGCCATTTTGAAAATAAAAGAAAATACAATCAAATTGGCATGTCGCCAACGACAAAAGATTCACCGCCTCCAAAGCAAATGGCGGGGATGGAATAATCTCCATCGTATCGACGCTACTGAAGTGTTCCTACAACTCGATAGCGAAGACTATTCGTCTGATAATGCTTGGGAGGCGCTTGTGGTAAAGCCAAGCTTCAAGATTGTTGACCAATTTCTCTCTGAAAATAGTTTCAAACTTACAGAAAAGGTGTTCAACGCAGTTTTTTGA

mRNA sequence

ATGAAAATTTCGTTATCGACAAGCTGCAAGCAAGTTGCGGTGGAGCCGCCGCTGGTAAACTTTCAAGGCACAAACAACACACTGAATTTGGAACCATTTTTCCGGCGAAAAGAGAAAGTGGTATTTGTAATGGGGGCTACCGGCACTGGGAAATCACGGTTGGCAATTGATTTAGCCACTCGTTTCCCAGCTGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCAAAGTCCTCACCAATAAAGTCACTGAAAAAGAATGTCATGGCGTCCCACATCACCTCCTTGGAATCGCTGATCCCAATTCAAACTTCACTGTTGCCGACTTCCGCCGCCATGCCTCATCGGCGATCAACTCCATTATTATAGGAAAACGTCGTCTCCCTATTCTGGCCGGCGGCTCCAACTCCTTCATCGAATCACTTGTAGAGGAGGAGCCTGAGTTTCGTTACCGTTTTGATTGTTGCTTCTTATGGGTGGATGTTTCGTTACCCATCCTCAACTCGTTTGTGTCGGAACGTGTTGATCGGATGTTGGAAAATGGATTAGTTAATGAGGTACGGAATTTATTTGATCCTAATGGATTAGGAAATGATTATGGACGTGGAATTCGACGCGCAATTGGAGTTCCGGAACTTGACGCATTTCTACATGCTGAGATGGATTCTGCCACTGATGATAAAGCTCGATCTCGAATTCTTAAGGCTGCCATTTTGAAAATAAAAGAAAATACAATCAAATTGGCATGTCGCCAACGACAAAAGATTCACCGCCTCCAAAGCAAATGGCGGGGATGGAATAATCTCCATCGTATCGACGCTACTGAAGTGTTCCTACAACTCGATAGCGAAGACTATTCGTCTGATAATGCTTGGGAGGCGCTTGTGGTAAAGCCAAGCTTCAAGATTGTTGACCAATTTCTCTCTGAAAATAGTTTCAAACTTACAGAAAAGGTGTTCAACGCAGTTTTTTGA

Coding sequence (CDS)

ATGAAAATTTCGTTATCGACAAGCTGCAAGCAAGTTGCGGTGGAGCCGCCGCTGGTAAACTTTCAAGGCACAAACAACACACTGAATTTGGAACCATTTTTCCGGCGAAAAGAGAAAGTGGTATTTGTAATGGGGGCTACCGGCACTGGGAAATCACGGTTGGCAATTGATTTAGCCACTCGTTTCCCAGCTGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCAAAGTCCTCACCAATAAAGTCACTGAAAAAGAATGTCATGGCGTCCCACATCACCTCCTTGGAATCGCTGATCCCAATTCAAACTTCACTGTTGCCGACTTCCGCCGCCATGCCTCATCGGCGATCAACTCCATTATTATAGGAAAACGTCGTCTCCCTATTCTGGCCGGCGGCTCCAACTCCTTCATCGAATCACTTGTAGAGGAGGAGCCTGAGTTTCGTTACCGTTTTGATTGTTGCTTCTTATGGGTGGATGTTTCGTTACCCATCCTCAACTCGTTTGTGTCGGAACGTGTTGATCGGATGTTGGAAAATGGATTAGTTAATGAGGTACGGAATTTATTTGATCCTAATGGATTAGGAAATGATTATGGACGTGGAATTCGACGCGCAATTGGAGTTCCGGAACTTGACGCATTTCTACATGCTGAGATGGATTCTGCCACTGATGATAAAGCTCGATCTCGAATTCTTAAGGCTGCCATTTTGAAAATAAAAGAAAATACAATCAAATTGGCATGTCGCCAACGACAAAAGATTCACCGCCTCCAAAGCAAATGGCGGGGATGGAATAATCTCCATCGTATCGACGCTACTGAAGTGTTCCTACAACTCGATAGCGAAGACTATTCGTCTGATAATGCTTGGGAGGCGCTTGTGGTAAAGCCAAGCTTCAAGATTGTTGACCAATTTCTCTCTGAAAATAGTTTCAAACTTACAGAAAAGGTGTTCAACGCAGTTTTTTGA

Protein sequence

MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAINSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVKPSFKIVDQFLSENSFKLTEKVFNAVF*
Homology
BLAST of Chy11G200120 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 339.3 bits (869), Expect = 4.7e-92
Identity = 184/306 (60.13%), Postives = 234/306 (76.47%), Query Frame = 0

Query: 7   TSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLATRFPAEI 66
           T+ +QV ++P  +NFQG  N +++ PFFRRK+KVVFVMGATGTGKSRLAIDLATRFPAEI
Sbjct: 6   TALRQV-IQPLSLNFQG--NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI 65

Query: 67  INSDKIQVYKGLKVLTNKVTEKECHGVPHHLLG-IADPNSNFTVADFRRHASSAINSIII 126
           +NSDKIQVYKGL ++TNKVT +E  GVPHHLLG + D   +FT  DF+R A  A+ S I+
Sbjct: 66  VNSDKIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVES-IV 125

Query: 127 GKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRMLENGL 186
            + R+PI+AGGSNS+IE+LV +  +FR R++CCFLWVDVS P+L+SFVSERVD+M++ GL
Sbjct: 126 QRDRVPIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGL 185

Query: 187 VNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAILKIKE 246
           V+EVR +FDP+   +DY  GIRRAIGVPELD FL +EM +   +    R+L+ AI KIKE
Sbjct: 186 VDEVRRIFDPS--SSDYSAGIRRAIGVPELDEFLRSEMRNYPAE-TTERLLETAIEKIKE 245

Query: 247 NTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVKPSFKI 306
           NT  LACRQ QKI RL  +W+ W N+HR+DATEVFL+   E   +D AW+  V  PS   
Sbjct: 246 NTCLLACRQLQKIQRLYKQWK-W-NMHRVDATEVFLRRGEE---ADEAWDNSVAHPSALA 298

Query: 307 VDQFLS 312
           V++FLS
Sbjct: 306 VEKFLS 298

BLAST of Chy11G200120 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 319.7 bits (818), Expect = 3.8e-86
Identity = 177/316 (56.01%), Postives = 223/316 (70.57%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MK S+S S KQV    P++ F+   + +N+  F   KEKV+FVMGATG+GKSRLAIDLAT
Sbjct: 1   MKFSIS-SLKQV---QPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNS-NFTVADFRRHASSA 120
           RF  EIINSDKIQ+YKGL VLTNKVT KEC GVPHHLLG+ D  + N T   + R AS A
Sbjct: 61  RFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQA 120

Query: 121 INSIIIGKRRLPILAGGSNSFIESLVEEEPEFRY-RFDCCFLWVDVSLPILNSFVSERVD 180
           I S +    +LPI+AGGSNS+IE+LV     F    +DCCF+WVDVSLP+LNSFVS+RVD
Sbjct: 121 I-SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVD 180

Query: 181 RMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKA 240
           RM+E GL+ EVR +F+P     +Y  GIRRAIGVPEL  +L  E  S  D   +S++L  
Sbjct: 181 RMMEAGLLEEVREVFNPKA---NYSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDV 240

Query: 241 AILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALV 300
           A+  IK+NT  LACRQ +KI RL  KW+   ++HR+DATEVFL+ + E+   D AWE LV
Sbjct: 241 AVKNIKKNTEILACRQLKKIQRLHKKWK--MSMHRVDATEVFLKRNVEE--QDEAWENLV 300

Query: 301 VKPSFKIVDQFLSENS 315
            +PS +IVD+F + N+
Sbjct: 301 ARPSERIVDKFYNNNN 302

BLAST of Chy11G200120 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.2e-76
Identity = 157/314 (50.00%), Postives = 219/314 (69.75%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTN---NTLNLEPFFRRKEKVVFVMGATGTGKSRLAID 60
           MKIS++  CKQ     P ++F       N L L P +  K+KVV +MGATGTGKSRL++D
Sbjct: 3   MKISMA-MCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHAS 120
           +ATRF AEIINSDKIQV++GL ++TNK+T +E  GVPHHLLG+  P ++ T A++   A+
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 SAINSIIIGKRRLPILAGGSNSFIESLVEE-EPEFRYRFDCCFLWVDVSLPILNSFVSER 180
            +I S ++ + +LPI+ GGSNS++E+LV++ E +FR R+DCCFLWVDV+LP+L+ FVSER
Sbjct: 123 LSIES-VLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSER 182

Query: 181 VDRMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRIL 240
           VD+M+E+G+V EVR  FD +   +DY RGI++AIG PE D F   E     +D  R  +L
Sbjct: 183 VDKMVESGMVEEVREFFDFS--NSDYSRGIKKAIGFPEFDRFFRNEQFLNVED--REELL 242

Query: 241 KAAILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEA 300
              + +IK NT +LACRQR+KI RL+ K + W ++ R+DAT VF +  S+   ++ AWE 
Sbjct: 243 SKVLEEIKRNTFELACRQREKIERLR-KVKKW-SIQRVDATPVFTKRRSK-MDANVAWER 302

Query: 301 LVVKPSFKIVDQFL 311
           LV  PS   V +FL
Sbjct: 303 LVAGPSTDTVSRFL 306

BLAST of Chy11G200120 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.8e-60
Identity = 140/318 (44.03%), Postives = 184/318 (57.86%), Query Frame = 0

Query: 24  TNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKVLTN 83
           T   ++L     RKEK++ +MGATGTGKSRL+IDLA  FP E+INSDK+QVYKGL + TN
Sbjct: 16  TTTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTN 75

Query: 84  KVTEKECHGVPHHLLGIADP-NSNFTVADFRRHASSAINSIIIGKRRLPILAGGSNSFIE 143
           K++  +  GVPHHLLG  DP     T ADFR  A  A+ S I G+R+LP+L GGSNSFI 
Sbjct: 76  KISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAV-SEITGRRKLPVLVGGSNSFIH 135

Query: 144 SLVEEE----------------PEFRYRFDCCFLWVDVSLPILNSFVSERVDRMLENGLV 203
           +L+ +                      R+DCCFLWVDVS+ +L  ++++RVD MLE G+ 
Sbjct: 136 ALLVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMF 195

Query: 204 NEVRNLFDPNGLGNDYG----RGIRRAIGVPELDAFLH------AEMDSATDDKARSRIL 263
           +E+   + P    +D       G+R+AIGVPE D +         E +    D+ R    
Sbjct: 196 DELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAF 255

Query: 264 KAAILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSS--DNAW 313
           + A+  IKENT  LA RQ  KI RL  K  GW +L R+DATE F    + D        W
Sbjct: 256 EEAVRAIKENTCHLAKRQIGKILRL--KGAGW-DLRRLDATESFRAAMTSDSGEKCTEIW 315

BLAST of Chy11G200120 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 223.4 bits (568), Expect = 3.7e-57
Identity = 129/300 (43.00%), Postives = 185/300 (61.67%), Query Frame = 0

Query: 36  RKEKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPH 95
           RK+KVV ++GATG GKSRL++DLATRFP+EIINSDKIQVY+GL++ TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 96  HLLGIADP-NSNFTVADFRRHASSAINSIIIGKRRLPILAGGSNSFIESLVEE--EPEF- 155
           HLLG+ +P +   T  +FR  AS+ +   I  ++++PI+AGGSNSF+ +L+ +  +P+F 
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKE-ITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 182

Query: 156 -----------RYRFDCCFLWVDVSLPILNSFVSERVDRMLENGLVNEVRNLFDPNGLGN 215
                        R++CCF+WVDVS  +L  ++  RVD M+++G+  E+   +DP   G 
Sbjct: 183 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 242

Query: 216 DYGRGIRRAIGVPELDAFLH---AEMDSATDDKARSRILKAAILKIKENTIKLACRQRQK 275
           +   GIR+AIGVPE D +      E      D  R      A+  IK NT  LA RQ +K
Sbjct: 243 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 302

Query: 276 IHRLQSKWRGWNNLHRIDAT----EVFLQLDSEDYSSDNAWEALVVKPSFKIVDQFLSEN 314
           I  L  K  GW  + R+DAT     V ++  SE    +N WE  V++PS KIV + L +N
Sbjct: 303 IEML--KDAGW-EIERVDATASFKAVMMKSSSEKKWREN-WEEQVLEPSVKIVKRHLVQN 357

BLAST of Chy11G200120 vs. ExPASy TrEMBL
Match: A0A0A0KHK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.3e-177
Identity = 312/326 (95.71%), Postives = 319/326 (97.85%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MKISLSTSCKQ+AVEPPLVNFQGTNNTLNLEPFFRRK+KVVFVMGATGTGKSRLAIDLAT
Sbjct: 1   MKISLSTSCKQIAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQVYKGLKVLTNKVTEKEC GVPHHLLGIADPNSNFTVADFRRHASS I
Sbjct: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECCGVPHHLLGIADPNSNFTVADFRRHASSTI 120

Query: 121 NSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRM 180
           NSI++GKR LPILAGGSNSFIESLVEEEPEFRYR+DCCFLWVDVSLPILNSFVSERVDRM
Sbjct: 121 NSIVVGKRCLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAI 240
           LENGLVNEVR+LFDPNGLGNDYG GIRRAIGVPELDAFL AEMDSATDDK RSRILKAAI
Sbjct: 181 LENGLVNEVRSLFDPNGLGNDYGHGIRRAIGVPELDAFLRAEMDSATDDKTRSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVK 300
           LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWE LVVK
Sbjct: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWETLVVK 300

Query: 301 PSFKIVDQFLSENSFKLTEKVFNAVF 327
           PSFKIVDQFLSENSFKLTEKVF+AVF
Sbjct: 301 PSFKIVDQFLSENSFKLTEKVFSAVF 326

BLAST of Chy11G200120 vs. ExPASy TrEMBL
Match: A0A5A7STV9 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00780 PE=4 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 306/328 (93.29%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGT-NNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLA 60
           MKI LSTSCKQVAVEPPLVNFQGT NNTLNLEPFFRRK+KVVFVMGATGTGKSRLAIDLA
Sbjct: 1   MKIWLSTSCKQVAVEPPLVNFQGTNNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60

Query: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSA 120
           TRFPAEIINSDKIQVYKGLKVLTNKVTEKEC GVPHHLLGIADPNSNFTVADFRRHASSA
Sbjct: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120

Query: 121 INSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDR 180
           INSII+GKRRLPILAGGSNSFIESLVEEEPEFRYR+DCCFLWVDVSLPILNSFVSERVDR
Sbjct: 121 INSIIVGKRRLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDR 180

Query: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAA 240
           MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFL AEMDS TDDKARSRILKAA
Sbjct: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRAEMDSTTDDKARSRILKAA 240

Query: 241 ILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQL-DSEDYSSDNAWEALV 300
           ILKIKENTIKLACRQ+QKIHRLQ+KWRGWNN HRIDATE FLQL + E+YSSDNAWE LV
Sbjct: 241 ILKIKENTIKLACRQQQKIHRLQNKWRGWNNFHRIDATEAFLQLGNGENYSSDNAWETLV 300

Query: 301 VKPSFKIVDQFLSENSFKLTEKVFNAVF 327
           V+PS  I+DQFLS+NSFKLTEKVFNAVF
Sbjct: 301 VEPSSMIIDQFLSKNSFKLTEKVFNAVF 328

BLAST of Chy11G200120 vs. ExPASy TrEMBL
Match: A0A1S3C845 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103497923 PE=4 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 306/328 (93.29%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGT-NNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLA 60
           MKI LSTSCKQVAVEPPLVNFQGT NNTLNLEPFFRRK+KVVFVMGATGTGKSRLAIDLA
Sbjct: 1   MKIWLSTSCKQVAVEPPLVNFQGTNNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60

Query: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSA 120
           TRFPAEIINSDKIQVYKGLKVLTNKVTEKEC GVPHHLLGIADPNSNFTVADFRRHASSA
Sbjct: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120

Query: 121 INSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDR 180
           INSII+GKRRLPILAGGSNSFIESLVEEEPEFRYR+DCCFLWVDVSLPILNSFVSERVDR
Sbjct: 121 INSIIVGKRRLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDR 180

Query: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAA 240
           MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFL AEMDS TDDKARSRILKAA
Sbjct: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRAEMDSTTDDKARSRILKAA 240

Query: 241 ILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQL-DSEDYSSDNAWEALV 300
           ILKIKENTIKLACRQ+QKIHRLQ+KWRGWNN HRIDATE FLQL + E+YSSDNAWE LV
Sbjct: 241 ILKIKENTIKLACRQQQKIHRLQNKWRGWNNFHRIDATEAFLQLGNGENYSSDNAWETLV 300

Query: 301 VKPSFKIVDQFLSENSFKLTEKVFNAVF 327
           V+PS  I+DQFLS+NSFKLTEKVFNAVF
Sbjct: 301 VEPSSMIIDQFLSKNSFKLTEKVFNAVF 328

BLAST of Chy11G200120 vs. ExPASy TrEMBL
Match: A0A6J1H3C2 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460117 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 6.7e-142
Identity = 266/325 (81.85%), Postives = 288/325 (88.62%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQVA EPPLVN QGT N LNL+PFFR+K+K+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSASCKQVAAEPPLVNLQGT-NILNLKPFFRQKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL++LTNKVTEKEC GVPHHLLGIADPNSNFT  DFRRHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAI 120

Query: 121 NSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRM 180
           NS I+GK RLPI+AGGSNSFIESLV++E EFRYR+DCCFLWVDVSLP+L+SFVS+RVDRM
Sbjct: 121 NS-IVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAI 240
           L+NGLVNEVRNLFDP    NDY RGIRRAIGVPELDAFLHAEMDSATDDKARSRIL AAI
Sbjct: 181 LDNGLVNEVRNLFDPK--ENDYTRGIRRAIGVPELDAFLHAEMDSATDDKARSRILNAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVK 300
           LKIKEN+IKLA RQRQKIHRLQSKW GW NLHRIDATEVFLQ D  +Y SD+AWEALV +
Sbjct: 241 LKIKENSIKLALRQRQKIHRLQSKW-GW-NLHRIDATEVFLQHDGGEY-SDHAWEALVAE 300

Query: 301 PSFKIVDQFLSENSFKLTEKVFNAV 326
           PS  IVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAGKIPMAV 318

BLAST of Chy11G200120 vs. ExPASy TrEMBL
Match: A0A6J1KZJ7 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499016 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.9e-137
Identity = 259/325 (79.69%), Postives = 283/325 (87.08%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQ A E PLVNFQGT N LNL+PFFRRK+K+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSASCKQAATESPLVNFQGT-NILNLKPFFRRKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL++LTNKVTEKEC GVPHHLLGI DPNSNFT  DF RHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIVDPNSNFTATDFCRHASLAI 120

Query: 121 NSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRM 180
           NS I+GK RLPI+AGGSNSFIESLV+ E EFRYR+DCCFLWVDVSLP+L+SFVS+RVDRM
Sbjct: 121 NS-IVGKHRLPIIAGGSNSFIESLVDGEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAI 240
           L+NGLVNEVRNLFDP   GNDY RGIRRAIGVPELDAFL AEMDSATD+KARS+IL+AAI
Sbjct: 181 LDNGLVNEVRNLFDPK--GNDYTRGIRRAIGVPELDAFLRAEMDSATDNKARSQILQAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVK 300
           LKIKEN+IKLA RQRQKIH LQSKW GW NLHRIDATEVFLQ    +Y S++AWEALV +
Sbjct: 241 LKIKENSIKLALRQRQKIHHLQSKW-GW-NLHRIDATEVFLQHGGGEY-SEHAWEALVAE 300

Query: 301 PSFKIVDQFLSENSFKLTEKVFNAV 326
           PS  IVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAAKIPMAV 318

BLAST of Chy11G200120 vs. NCBI nr
Match: XP_004150320.1 (adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN47261.1 hypothetical protein Csa_017001 [Cucumis sativus])

HSP 1 Score: 628 bits (1620), Expect = 1.27e-226
Identity = 312/326 (95.71%), Postives = 319/326 (97.85%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MKISLSTSCKQ+AVEPPLVNFQGTNNTLNLEPFFRRK+KVVFVMGATGTGKSRLAIDLAT
Sbjct: 1   MKISLSTSCKQIAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQVYKGLKVLTNKVTEKEC GVPHHLLGIADPNSNFTVADFRRHASS I
Sbjct: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECCGVPHHLLGIADPNSNFTVADFRRHASSTI 120

Query: 121 NSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRM 180
           NSI++GKR LPILAGGSNSFIESLVEEEPEFRYR+DCCFLWVDVSLPILNSFVSERVDRM
Sbjct: 121 NSIVVGKRCLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAI 240
           LENGLVNEVR+LFDPNGLGNDYG GIRRAIGVPELDAFL AEMDSATDDK RSRILKAAI
Sbjct: 181 LENGLVNEVRSLFDPNGLGNDYGHGIRRAIGVPELDAFLRAEMDSATDDKTRSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVK 300
           LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWE LVVK
Sbjct: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWETLVVK 300

Query: 301 PSFKIVDQFLSENSFKLTEKVFNAVF 326
           PSFKIVDQFLSENSFKLTEKVF+AVF
Sbjct: 301 PSFKIVDQFLSENSFKLTEKVFSAVF 326

BLAST of Chy11G200120 vs. NCBI nr
Match: XP_008458554.1 (PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo] >KAA0033426.1 adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 608 bits (1569), Expect = 8.09e-219
Identity = 306/328 (93.29%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNN-TLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLA 60
           MKI LSTSCKQVAVEPPLVNFQGTNN TLNLEPFFRRK+KVVFVMGATGTGKSRLAIDLA
Sbjct: 1   MKIWLSTSCKQVAVEPPLVNFQGTNNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60

Query: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSA 120
           TRFPAEIINSDKIQVYKGLKVLTNKVTEKEC GVPHHLLGIADPNSNFTVADFRRHASSA
Sbjct: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120

Query: 121 INSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDR 180
           INSII+GKRRLPILAGGSNSFIESLVEEEPEFRYR+DCCFLWVDVSLPILNSFVSERVDR
Sbjct: 121 INSIIVGKRRLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDR 180

Query: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAA 240
           MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFL AEMDS TDDKARSRILKAA
Sbjct: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRAEMDSTTDDKARSRILKAA 240

Query: 241 ILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQL-DSEDYSSDNAWEALV 300
           ILKIKENTIKLACRQ+QKIHRLQ+KWRGWNN HRIDATE FLQL + E+YSSDNAWE LV
Sbjct: 241 ILKIKENTIKLACRQQQKIHRLQNKWRGWNNFHRIDATEAFLQLGNGENYSSDNAWETLV 300

Query: 301 VKPSFKIVDQFLSENSFKLTEKVFNAVF 326
           V+PS  I+DQFLS+NSFKLTEKVFNAVF
Sbjct: 301 VEPSSMIIDQFLSKNSFKLTEKVFNAVF 328

BLAST of Chy11G200120 vs. NCBI nr
Match: XP_038875913.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 533 bits (1373), Expect = 5.46e-189
Identity = 277/333 (83.18%), Postives = 299/333 (89.79%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MKISLS SCKQ A EPPLVNFQG+N TLNLEPFFRRK+KVVFVMGATG+GKSRLAIDLAT
Sbjct: 1   MKISLSASCKQAAAEPPLVNFQGSN-TLNLEPFFRRKDKVVFVMGATGSGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQVYKGL++LTNKVTEKEC GVPHHLLGIADPNSNFT ADFRRHASSAI
Sbjct: 61  RFPAEIINSDKIQVYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAI 120

Query: 121 NSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRM 180
           NSI+ GKRRLPI+AGGSNSFIESLV++EPEFRYR+DCCFLWVDVSLP+L+SFVSERVDRM
Sbjct: 121 NSIV-GKRRLPIVAGGSNSFIESLVDDEPEFRYRYDCCFLWVDVSLPVLHSFVSERVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAI 240
           L++GLVNEVRNLFDP G  NDY RGIRRAIGVPELDAFLHAEMDSA+DDK RSRILKAAI
Sbjct: 181 LDDGLVNEVRNLFDPKG--NDYARGIRRAIGVPELDAFLHAEMDSASDDKTRSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVK 300
           LKIKENT KLACRQRQ IHRLQSKW GWN LHRIDATEVFLQL + +YS+ + WE LV +
Sbjct: 241 LKIKENTRKLACRQRQNIHRLQSKW-GWN-LHRIDATEVFLQLGNGEYSN-HVWETLVAE 300

Query: 301 PSFKIVDQFLSENSFKL-------TEKVFNAVF 326
           PS  I+DQFL ENSFK        T+KVFN VF
Sbjct: 301 PSSIIIDQFLCENSFKRHSVKIEHTDKVFNVVF 326

BLAST of Chy11G200120 vs. NCBI nr
Match: XP_022958992.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 510 bits (1313), Expect = 6.95e-180
Identity = 266/325 (81.85%), Postives = 288/325 (88.62%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQVA EPPLVN QGTN  LNL+PFFR+K+K+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSASCKQVAAEPPLVNLQGTN-ILNLKPFFRQKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL++LTNKVTEKEC GVPHHLLGIADPNSNFT  DFRRHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAI 120

Query: 121 NSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRM 180
           NSI+ GK RLPI+AGGSNSFIESLV++E EFRYR+DCCFLWVDVSLP+L+SFVS+RVDRM
Sbjct: 121 NSIV-GKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAI 240
           L+NGLVNEVRNLFDP    NDY RGIRRAIGVPELDAFLHAEMDSATDDKARSRIL AAI
Sbjct: 181 LDNGLVNEVRNLFDPKE--NDYTRGIRRAIGVPELDAFLHAEMDSATDDKARSRILNAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVK 300
           LKIKEN+IKLA RQRQKIHRLQSKW GWN LHRIDATEVFLQ D  +YS D+AWEALV +
Sbjct: 241 LKIKENSIKLALRQRQKIHRLQSKW-GWN-LHRIDATEVFLQHDGGEYS-DHAWEALVAE 300

Query: 301 PSFKIVDQFLSENSFKLTEKVFNAV 325
           PS  IVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAGKIPMAV 318

BLAST of Chy11G200120 vs. NCBI nr
Match: KAG6575015.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 509 bits (1310), Expect = 1.99e-179
Identity = 266/325 (81.85%), Postives = 288/325 (88.62%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQVA EPPLVNFQGTN  LNL+PFFR+K+K+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSVSCKQVAAEPPLVNFQGTN-ILNLKPFFRQKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL++LTNKVTEKEC GVPHHLLGIADPNSNFT  DFRRHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAI 120

Query: 121 NSIIIGKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRM 180
           NSI+ GK RLPI+AGGSNSFIESLV++E EFRYR+DCCFLWVDVSLP+L+SFVS+RVDRM
Sbjct: 121 NSIV-GKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAI 240
           L+NGLVNEVRNLFDP    NDY RGIRRAIGVPELDAFL AEMDSATDDKARSRILKAAI
Sbjct: 181 LDNGLVNEVRNLFDPKE--NDYTRGIRRAIGVPELDAFLRAEMDSATDDKARSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVK 300
           LKIKEN+IKLA RQRQKIHRLQSKW GWN LHRIDATEVFLQ    +YS D+AWEALV +
Sbjct: 241 LKIKENSIKLALRQRQKIHRLQSKW-GWN-LHRIDATEVFLQHGGGEYS-DHAWEALVAE 300

Query: 301 PSFKIVDQFLSENSFKLTEKVFNAV 325
           PS  IVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAAKIPMAV 318

BLAST of Chy11G200120 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 339.3 bits (869), Expect = 3.3e-93
Identity = 184/306 (60.13%), Postives = 234/306 (76.47%), Query Frame = 0

Query: 7   TSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLATRFPAEI 66
           T+ +QV ++P  +NFQG  N +++ PFFRRK+KVVFVMGATGTGKSRLAIDLATRFPAEI
Sbjct: 6   TALRQV-IQPLSLNFQG--NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI 65

Query: 67  INSDKIQVYKGLKVLTNKVTEKECHGVPHHLLG-IADPNSNFTVADFRRHASSAINSIII 126
           +NSDKIQVYKGL ++TNKVT +E  GVPHHLLG + D   +FT  DF+R A  A+ S I+
Sbjct: 66  VNSDKIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVES-IV 125

Query: 127 GKRRLPILAGGSNSFIESLVEEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRMLENGL 186
            + R+PI+AGGSNS+IE+LV +  +FR R++CCFLWVDVS P+L+SFVSERVD+M++ GL
Sbjct: 126 QRDRVPIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGL 185

Query: 187 VNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKAAILKIKE 246
           V+EVR +FDP+   +DY  GIRRAIGVPELD FL +EM +   +    R+L+ AI KIKE
Sbjct: 186 VDEVRRIFDPS--SSDYSAGIRRAIGVPELDEFLRSEMRNYPAE-TTERLLETAIEKIKE 245

Query: 247 NTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVKPSFKI 306
           NT  LACRQ QKI RL  +W+ W N+HR+DATEVFL+   E   +D AW+  V  PS   
Sbjct: 246 NTCLLACRQLQKIQRLYKQWK-W-NMHRVDATEVFLRRGEE---ADEAWDNSVAHPSALA 298

Query: 307 VDQFLS 312
           V++FLS
Sbjct: 306 VEKFLS 298

BLAST of Chy11G200120 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 319.7 bits (818), Expect = 2.7e-87
Identity = 177/316 (56.01%), Postives = 223/316 (70.57%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKEKVVFVMGATGTGKSRLAIDLAT 60
           MK S+S S KQV    P++ F+   + +N+  F   KEKV+FVMGATG+GKSRLAIDLAT
Sbjct: 1   MKFSIS-SLKQV---QPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNS-NFTVADFRRHASSA 120
           RF  EIINSDKIQ+YKGL VLTNKVT KEC GVPHHLLG+ D  + N T   + R AS A
Sbjct: 61  RFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQA 120

Query: 121 INSIIIGKRRLPILAGGSNSFIESLVEEEPEFRY-RFDCCFLWVDVSLPILNSFVSERVD 180
           I S +    +LPI+AGGSNS+IE+LV     F    +DCCF+WVDVSLP+LNSFVS+RVD
Sbjct: 121 I-SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVD 180

Query: 181 RMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRILKA 240
           RM+E GL+ EVR +F+P     +Y  GIRRAIGVPEL  +L  E  S  D   +S++L  
Sbjct: 181 RMMEAGLLEEVREVFNPKA---NYSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDV 240

Query: 241 AILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALV 300
           A+  IK+NT  LACRQ +KI RL  KW+   ++HR+DATEVFL+ + E+   D AWE LV
Sbjct: 241 AVKNIKKNTEILACRQLKKIQRLHKKWK--MSMHRVDATEVFLKRNVEE--QDEAWENLV 300

Query: 301 VKPSFKIVDQFLSENS 315
            +PS +IVD+F + N+
Sbjct: 301 ARPSERIVDKFYNNNN 302

BLAST of Chy11G200120 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 288.1 bits (736), Expect = 8.8e-78
Identity = 157/314 (50.00%), Postives = 219/314 (69.75%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTN---NTLNLEPFFRRKEKVVFVMGATGTGKSRLAID 60
           MKIS++  CKQ     P ++F       N L L P +  K+KVV +MGATGTGKSRL++D
Sbjct: 3   MKISMA-MCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHHLLGIADPNSNFTVADFRRHAS 120
           +ATRF AEIINSDKIQV++GL ++TNK+T +E  GVPHHLLG+  P ++ T A++   A+
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 SAINSIIIGKRRLPILAGGSNSFIESLVEE-EPEFRYRFDCCFLWVDVSLPILNSFVSER 180
            +I S ++ + +LPI+ GGSNS++E+LV++ E +FR R+DCCFLWVDV+LP+L+ FVSER
Sbjct: 123 LSIES-VLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSER 182

Query: 181 VDRMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLHAEMDSATDDKARSRIL 240
           VD+M+E+G+V EVR  FD +   +DY RGI++AIG PE D F   E     +D  R  +L
Sbjct: 183 VDKMVESGMVEEVREFFDFS--NSDYSRGIKKAIGFPEFDRFFRNEQFLNVED--REELL 242

Query: 241 KAAILKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEA 300
              + +IK NT +LACRQR+KI RL+ K + W ++ R+DAT VF +  S+   ++ AWE 
Sbjct: 243 SKVLEEIKRNTFELACRQREKIERLR-KVKKW-SIQRVDATPVFTKRRSK-MDANVAWER 302

Query: 301 LVVKPSFKIVDQFL 311
           LV  PS   V +FL
Sbjct: 303 LVAGPSTDTVSRFL 306

BLAST of Chy11G200120 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 223.4 bits (568), Expect = 2.7e-58
Identity = 129/300 (43.00%), Postives = 185/300 (61.67%), Query Frame = 0

Query: 36  RKEKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPH 95
           RK+KVV ++GATG GKSRL++DLATRFP+EIINSDKIQVY+GL++ TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 96  HLLGIADP-NSNFTVADFRRHASSAINSIIIGKRRLPILAGGSNSFIESLVEE--EPEF- 155
           HLLG+ +P +   T  +FR  AS+ +   I  ++++PI+AGGSNSF+ +L+ +  +P+F 
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKE-ITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 182

Query: 156 -----------RYRFDCCFLWVDVSLPILNSFVSERVDRMLENGLVNEVRNLFDPNGLGN 215
                        R++CCF+WVDVS  +L  ++  RVD M+++G+  E+   +DP   G 
Sbjct: 183 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 242

Query: 216 DYGRGIRRAIGVPELDAFLH---AEMDSATDDKARSRILKAAILKIKENTIKLACRQRQK 275
           +   GIR+AIGVPE D +      E      D  R      A+  IK NT  LA RQ +K
Sbjct: 243 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 302

Query: 276 IHRLQSKWRGWNNLHRIDAT----EVFLQLDSEDYSSDNAWEALVVKPSFKIVDQFLSEN 314
           I  L  K  GW  + R+DAT     V ++  SE    +N WE  V++PS KIV + L +N
Sbjct: 303 IEML--KDAGW-EIERVDATASFKAVMMKSSSEKKWREN-WEEQVLEPSVKIVKRHLVQN 357

BLAST of Chy11G200120 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 211.1 bits (536), Expect = 1.4e-54
Identity = 127/294 (43.20%), Postives = 178/294 (60.54%), Query Frame = 0

Query: 37  KEKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKVLTNKVTEKECHGVPHH 96
           K+KVV +MGATG+GKS L+IDLATRF  EI+NSDKIQ Y GLKV TN+++  E  GVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 97  LLGIADP-NSNFTVADFRRHASSAINSIIIGKRRLPILAGGSNSFIESLV---------- 156
           LLG   P +S  T ++FR  AS +I S I  +  LPI+AGGSNSFI +L+          
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSI-SEITARGNLPIIAGGSNSFIHALLVDRFDPKTYP 161

Query: 157 ---EEEPEFRYRFDCCFLWVDVSLPILNSFVSERVDRMLENGLVNEVRNLFDPNGLGNDY 216
              E       R++CCFLWVDVS+ +L  ++S+RVD+M+E+G+  E+   +DP   G+  
Sbjct: 162 FSSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAI 221

Query: 217 -GRGIRRAIGVPELDAFL-----HAEMDSATDDKARSRILKAAILKIKENTIKLACRQRQ 276
              GI + IG+PE D +        +   +  D+AR      A+ +IKENT +LA +Q +
Sbjct: 222 RAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIE 281

Query: 277 KIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWEALVVKPSFKIVDQFL 311
           +I +L+S   GW ++ R+DAT  F        SS   W+  V+  S K+V +FL
Sbjct: 282 RIMKLKSS--GW-DIQRLDATPSF------GRSSREIWDNTVLDESIKVVKRFL 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94ID24.7e-9260.13Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID13.8e-8656.01Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q93WC91.2e-7650.00Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q5GHF72.8e-6044.03Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID33.7e-5743.00Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A0A0KHK31.3e-17795.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1[more]
A0A5A7STV91.1e-17193.29Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3C8451.1e-17193.29adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 G... [more]
A0A6J1H3C26.7e-14281.85adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1KZJ72.9e-13779.69adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_004150320.11.27e-22695.71adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN47261.1 ... [more]
XP_008458554.18.09e-21993.29PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo]... [more]
XP_038875913.15.46e-18983.18adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida][more]
XP_022958992.16.95e-18081.85adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata][more]
KAG6575015.11.99e-17981.85Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT5G19040.13.3e-9360.13isopentenyltransferase 5 [more]
AT3G23630.12.7e-8756.01isopentenyltransferase 7 [more]
AT3G63110.18.8e-7850.00isopentenyltransferase 3 [more]
AT1G68460.12.7e-5843.00isopentenyltransferase 1 [more]
AT3G19160.11.4e-5443.20ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 166..271
e-value: 3.9E-105
score: 352.9
NoneNo IPR availablePFAMPF01715IPPTcoord: 153..258
e-value: 8.4E-11
score: 42.0
coord: 73..148
e-value: 9.2E-21
score: 74.7
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 28..316
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 37..310
e-value: 3.9E-105
score: 352.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 40..148
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 28..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G200120.1Chy11G200120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity