Chy11G198870 (gene) Cucumber (hystrix) v1

Overview


Name	Chy11G198870
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	NEDD8-specific protease 1
Location	chrH11: 19320997 .. 19321707 (+)
RNA-Seq Expression	Chy11G198870
Synteny	Chy11G198870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAGTCTGAAGGAGATGAGAAAATCTTAAGCTACAACGATGTTGTACTCAGAAAATCCGACTTGGAGATCCTTAGCGGTCCATATTTCCTGAACGATCGAATCATCGAGTTCTATTTTAGCTATCTAAGCACAACCCATCCTTCCAACGACGTTCTTCTATCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCCGATGTCGAAAGTCTCAACAGTTTTCTAGAACCTCTCAATCTACCCCACAAGAAGCTCGTAATCTTCCCTGTAAACGACAACATTGACGTTAGTAAACCTGAAGGTGGAAACCACTGGAGTCTTCTTGCATTTTACAGAGAAGCAAACATATTTGTTCATCATGATAGCAATAAAGGAATGAACAAGTATGCCGCCAAGCGACTCTACAAAGCCGTGGCTAGATTTATGAATGATGACTCGAATTCAACATCGAATCCAAGTTACGTGGAATGCATCGAGTCTCCACAACAAGTAAATGGATATGATTGTGGTGTGTATGTTACAGCTATAGCAAGAAGCATATGCAAATGGTATGAGGAAAGGAGAGAAGTTGAGACAGATGGGTTGTGGTTTTCAGCTGTGGTGGAGGAGATTAGTCCATTATTGGTTGCTAATATGCGTAGAGAAATTTTGGGGCTAATAAGAAGTTTAATGGCTGTGAAGGAATATCAACCGGCTACTAAATAA

mRNA sequence

ATGGGGAAGTCTGAAGGAGATGAGAAAATCTTAAGCTACAACGATGTTGTACTCAGAAAATCCGACTTGGAGATCCTTAGCGGTCCATATTTCCTGAACGATCGAATCATCGAGTTCTATTTTAGCTATCTAAGCACAACCCATCCTTCCAACGACGTTCTTCTATCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCCGATGTCGAAAGTCTCAACAGTTTTCTAGAACCTCTCAATCTACCCCACAAGAAGCTCGTAATCTTCCCTGTAAACGACAACATTGACGTTAGTAAACCTGAAGGTGGAAACCACTGGAGTCTTCTTGCATTTTACAGAGAAGCAAACATATTTGTTCATCATGATAGCAATAAAGGAATGAACAAGTATGCCGCCAAGCGACTCTACAAAGCCGTGGCTAGATTTATGAATGATGACTCGAATTCAACATCGAATCCAAGTTACGTGGAATGCATCGAGTCTCCACAACAAGTAAATGGATATGATTGTGGTGTGTATGTTACAGCTATAGCAAGAAGCATATGCAAATGGTATGAGGAAAGGAGAGAAGTTGAGACAGATGGGTTGTGGTTTTCAGCTGTGGTGGAGGAGATTAGTCCATTATTGGTTGCTAATATGCGTAGAGAAATTTTGGGGCTAATAAGAAGTTTAATGGCTGTGAAGGAATATCAACCGGCTACTAAATAA

Coding sequence (CDS)

ATGGGGAAGTCTGAAGGAGATGAGAAAATCTTAAGCTACAACGATGTTGTACTCAGAAAATCCGACTTGGAGATCCTTAGCGGTCCATATTTCCTGAACGATCGAATCATCGAGTTCTATTTTAGCTATCTAAGCACAACCCATCCTTCCAACGACGTTCTTCTATCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCCGATGTCGAAAGTCTCAACAGTTTTCTAGAACCTCTCAATCTACCCCACAAGAAGCTCGTAATCTTCCCTGTAAACGACAACATTGACGTTAGTAAACCTGAAGGTGGAAACCACTGGAGTCTTCTTGCATTTTACAGAGAAGCAAACATATTTGTTCATCATGATAGCAATAAAGGAATGAACAAGTATGCCGCCAAGCGACTCTACAAAGCCGTGGCTAGATTTATGAATGATGACTCGAATTCAACATCGAATCCAAGTTACGTGGAATGCATCGAGTCTCCACAACAAGTAAATGGATATGATTGTGGTGTGTATGTTACAGCTATAGCAAGAAGCATATGCAAATGGTATGAGGAAAGGAGAGAAGTTGAGACAGATGGGTTGTGGTTTTCAGCTGTGGTGGAGGAGATTAGTCCATTATTGGTTGCTAATATGCGTAGAGAAATTTTGGGGCTAATAAGAAGTTTAATGGCTGTGAAGGAATATCAACCGGCTACTAAATAA

Protein sequence

MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSIAFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFVHHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARSICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK*

Homology

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BLAST of Chy11G198870 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-70
Identity = 130/227 (57.27%), Postives = 164/227 (72.25%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MG +  D+KILSY DVVLR+SDL+IL+GP FLNDR+IEFY S+LST H S  + L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWI NCPD E L  F++PLNL  K L+I PVNDN +V   EGG HWSLL +Y+EAN FV
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS  G+N+++AK+L+KAV+ F+     S  + SY EC ++PQQ NGYDCGV++ A AR
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV-----SNGDASYKECTDTPQQKNGYDCGVFLLATAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMA 228
            IC+W+        D LWF A V+E  P LV ++R EIL LI+ LM+
Sbjct: 181 VICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

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BLAST of Chy11G198870 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 5.5e-26
Identity = 73/227 (32.16%), Postives = 116/227 (51.10%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTT--HPSND-VLLSPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y + +  H  +D V    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQF 61

Query: 67  IMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFVHHD 126
           I        +  FLEPL+LPHK++V   +NDN   ++  GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDN--SNQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARSIC 186
           S+   N   AK++ + +  F+    +      +VE  ++P Q N YDCG+YV     ++C
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLGSKGDKL---VFVE-EKAPAQENSYDCGMYVICNTEALC 181

Query: 187 KWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKE 231
           +    R+          + ++ ++P  +   R E   LI  L    E
Sbjct: 182 QSLFRRQP--------ESPLQLLTPTYITKKRGEWKDLIARLAKKNE 214

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BLAST of Chy11G198870 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.5e-26
Identity = 65/181 (35.91%), Postives = 102/181 (56.35%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTT--HPSND-VLLSPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y +++  H  +D V    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQF 61

Query: 67  IMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFVHHD 126
           I        +  FLEPL+LPHK++V   +NDN   ++  GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDN--SNQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARSIC 185
           S+   N   AK++ + +  F+    +      +VE  ++P Q N YDCG+YV     ++C
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLGSKGDKL---VFVE-EKAPAQQNSYDCGMYVICNTEALC 176

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BLAST of Chy11G198870 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 72/222 (32.43%), Postives = 122/222 (54.95%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTT--HPSND-VLLSPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y + +  H  +D V    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQF 61

Query: 67  IMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFVHHD 126
           I    +   +  FLEPL+LP+K++V   +NDN   ++  GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSNPAEIAMFLEPLDLPNKRVVFLAINDN--SNQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARSIC 186
           S+   N   AK++ + +  F+    +     ++VE  ++P Q N YDCG+YV     ++C
Sbjct: 122 SHSRSNSVHAKQVAEKLEAFLGRKGDKL---AFVE-EKAPAQQNSYDCGMYVICNTEALC 181

Query: 187 KWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSL 226
           + +  R++ E       ++++ ++P  +   R E   LI +L
Sbjct: 182 QNF-FRQQTE-------SLLQLLTPAYITKKRGEWKDLITTL 209

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BLAST of Chy11G198870 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.4e-24
Identity = 78/242 (32.23%), Postives = 116/242 (47.93%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYL------STTHPSNDVLLSPPSI 66
           D  I+SYND  + +SD+ IL    +LND II FY  +L      +     N VLL  PS+
Sbjct: 3   DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSV 62

Query: 67  AFWIMNCPDVESLNS----FLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREA 126
            F    C  VES       F +PL+L  K+++ FP+ +N D +   GG HWSLL F +  
Sbjct: 63  VFC---CSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSL 122

Query: 127 NIFVHHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVT 186
           N F+++DS    N   A  +       ++     T+   ++   ++PQQ NGYDCG+YV 
Sbjct: 123 NKFIYYDSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVL 182

Query: 187 AIARSICKWY--------EERREVETDGLWFS-----AVVEEISPLLVANMRREILGLIR 226
           +I   + K           E  ++    L  S      + +EI+P  + + R EIL  I 
Sbjct: 183 SIIEELLKLIIKENENNKGEENKISYKDLLLSEFTNELLFKEITPNYIKDKRVEILNTIL 241

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BLAST of Chy11G198870 vs. ExPASy TrEMBL
Match: A0A0A0KEM9 (ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G289720 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.8e-128
Identity = 226/236 (95.76%), Postives = 229/236 (97.03%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSN +LLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNGILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFW+MNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV
Sbjct: 61  AFWMMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSNKGMNKYAAKRLY AVARFMND S ST NPSYVEC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYAAKRLYNAVARFMNDGSISTLNPSYVECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 237
           SICKWYEERREVE DGLWFSAVVEEISP LVANMRREILGLIRSLMAVKEYQPATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPSLVANMRREILGLIRSLMAVKEYQPATK 236

[Close]

BLAST of Chy11G198870 vs. ExPASy TrEMBL
Match: A0A5D3CSG4 (NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00810 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.6e-127
Identity = 224/236 (94.92%), Postives = 228/236 (96.61%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGK EGDEKILS+NDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSND+LLSPPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWIMNCPDVESLN FLEPLNL HKKLVIFPVNDN DVSKPEGGNHWSLLAFYREANIFV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSNKGMNKY AKRLYKAVARFMND SNSTSNPSY+EC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 237
           SICKWYEERREVE DGLWFSAVVEEISPLLVANMRREIL LIRSLMAVKEYQPATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Chy11G198870 vs. ExPASy TrEMBL
Match: A0A1S3CK81 (NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.6e-127
Identity = 224/236 (94.92%), Postives = 228/236 (96.61%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGK EGDEKILS+NDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSND+LLSPPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWIMNCPDVESLN FLEPLNL HKKLVIFPVNDN DVSKPEGGNHWSLLAFYREANIFV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSNKGMNKY AKRLYKAVARFMND SNSTSNPSY+EC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 237
           SICKWYEERREVE DGLWFSAVVEEISPLLVANMRREIL LIRSLMAVKEYQPATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Chy11G198870 vs. ExPASy TrEMBL
Match: A0A6J1CWB2 (NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 4.3e-106
Identity = 186/236 (78.81%), Postives = 210/236 (88.98%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           M KSEGD+KILSYNDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLS+ HPS D+LL+PPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFW MNCPD  SL+ FL PL+L +KKLVIFPVNDN DVSK EGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSN+GMNK+ AKRLYKAVARF ++DSN TS P YVEC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 237
           SIC WY +R+EV+ DG WF AVVEE++P  VANMR EIL LIRSLMAVKEYQPA++
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

[Close]

BLAST of Chy11G198870 vs. ExPASy TrEMBL
Match: A0A6J1KYI9 (NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 2.7e-100
Identity = 181/239 (75.73%), Postives = 206/239 (86.19%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDL+IL+GP +LNDRIIEFYFSYL T HPS D+LLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWIMNCPD + LN FL+PLNL  + LVIFPVNDN DVSK EGGNHWS+L FYREAN+FV
Sbjct: 61  AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS KG+N  +AKRLY+AVARFM   +NSTS PSYVEC ESPQQVNG+DCG+YVTAI+R
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFMG-VTNSTSEPSYVECSESPQQVNGHDCGLYVTAISR 180

Query: 181 SICKWYEERREVETDG---LWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 237
           SIC+WYE+ R+    G   LWFSAVV+E++P  VA+MR EILGLIRS MA+KEYQPA+K
Sbjct: 181 SICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK 238

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BLAST of Chy11G198870 vs. NCBI nr
Match: XP_004149559.1 (NEDD8-specific protease 1 [Cucumis sativus] >XP_031742683.1 NEDD8-specific protease 1 [Cucumis sativus] >XP_031742684.1 NEDD8-specific protease 1 [Cucumis sativus] >KGN47289.1 hypothetical protein Csa_022955 [Cucumis sativus])

HSP 1 Score: 466 bits (1199), Expect = 1.81e-165
Identity = 226/236 (95.76%), Postives = 229/236 (97.03%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSN +LLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNGILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFW+MNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV
Sbjct: 61  AFWMMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSNKGMNKYAAKRLY AVARFMND S ST NPSYVEC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYAAKRLYNAVARFMNDGSISTLNPSYVECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 236
           SICKWYEERREVE DGLWFSAVVEEISP LVANMRREILGLIRSLMAVKEYQPATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPSLVANMRREILGLIRSLMAVKEYQPATK 236

[Close]

BLAST of Chy11G198870 vs. NCBI nr
Match: XP_008463889.1 (PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa] >TYK14342.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 462 bits (1189), Expect = 6.05e-164
Identity = 224/236 (94.92%), Postives = 228/236 (96.61%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGK EGDEKILS+NDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSND+LLSPPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWIMNCPDVESLN FLEPLNL HKKLVIFPVNDN DVSKPEGGNHWSLLAFYREANIFV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSNKGMNKY AKRLYKAVARFMND SNSTSNPSY+EC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 236
           SICKWYEERREVE DGLWFSAVVEEISPLLVANMRREIL LIRSLMAVKEYQPATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Chy11G198870 vs. NCBI nr
Match: XP_038902669.1 (NEDD8-specific protease 1 [Benincasa hispida])

HSP 1 Score: 423 bits (1087), Expect = 2.14e-148
Identity = 200/236 (84.75%), Postives = 219/236 (92.80%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDL+IL GPY+LNDRI EFYFSYLSTTHPS D+LLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILGGPYYLNDRIPEFYFSYLSTTHPSKDILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWIMNCPD ESL+ FL+PLNL  KKLVIFPVNDN DVSK EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWIMNCPDAESLDDFLKPLNLCDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS+KGMNKY AKRLY+AVARFM+ DSNSTSNPSY+EC+ESPQQVNGYDCGVYV AIAR
Sbjct: 121 HHDSHKGMNKYHAKRLYRAVARFMSADSNSTSNPSYLECVESPQQVNGYDCGVYVIAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 236
           +IC+WYE+R+EV+ DGLWFSAVV+E+SP  V NMR EILGLIRSLMAVKEYQPA+K
Sbjct: 181 TICRWYEQRKEVDRDGLWFSAVVKEVSPSFVTNMRSEILGLIRSLMAVKEYQPASK 236

[Close]

BLAST of Chy11G198870 vs. NCBI nr
Match: XP_022146090.1 (NEDD8-specific protease 1 [Momordica charantia])

HSP 1 Score: 392 bits (1006), Expect = 4.70e-136
Identity = 186/236 (78.81%), Postives = 210/236 (88.98%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           M KSEGD+KILSYNDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLS+ HPS D+LL+PPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFW MNCPD  SL+ FL PL+L +KKLVIFPVNDN DVSK EGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSN+GMNK+ AKRLYKAVARF ++DSN TS P YVEC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 236
           SIC WY +R+EV+ DG WF AVVEE++P  VANMR EIL LIRSLMAVKEYQPA++
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

[Close]

BLAST of Chy11G198870 vs. NCBI nr
Match: XP_023007377.1 (NEDD8-specific protease 1 [Cucurbita maxima])

HSP 1 Score: 372 bits (956), Expect = 2.09e-128
Identity = 181/239 (75.73%), Postives = 206/239 (86.19%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDL+IL+GP +LNDRIIEFYFSYL T HPS D+LLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWIMNCPD + LN FL+PLNL  + LVIFPVNDN DVSK EGGNHWS+L FYREAN+FV
Sbjct: 61  AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS KG+N  +AKRLY+AVARFM   +NSTS PSYVEC ESPQQVNG+DCG+YVTAI+R
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFMGV-TNSTSEPSYVECSESPQQVNGHDCGLYVTAISR 180

Query: 181 SICKWYEERREVETDG---LWFSAVVEEISPLLVANMRREILGLIRSLMAVKEYQPATK 236
           SIC+WYE+ R+    G   LWFSAVV+E++P  VA+MR EILGLIRS MA+KEYQPA+K
Sbjct: 181 SICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK 238

[Close]

BLAST of Chy11G198870 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 8.9e-72
Identity = 130/227 (57.27%), Postives = 164/227 (72.25%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSI 60
           MG +  D+KILSY DVVLR+SDL+IL+GP FLNDR+IEFY S+LST H S  + L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWIMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120
           AFWI NCPD E L  F++PLNL  K L+I PVNDN +V   EGG HWSLL +Y+EAN FV
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNKGMNKYAAKRLYKAVARFMNDDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS  G+N+++AK+L+KAV+ F+     S  + SY EC ++PQQ NGYDCGV++ A AR
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV-----SNGDASYKECTDTPQQKNGYDCGVFLLATAR 180

Query: 181 SICKWYEERREVETDGLWFSAVVEEISPLLVANMRREILGLIRSLMA 228
            IC+W+        D LWF A V+E  P LV ++R EIL LI+ LM+
Sbjct: 181 VICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

[Close]

BLAST of Chy11G198870 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 3.8e-06
Identity = 48/210 (22.86%), Postives = 79/210 (37.62%), Query Frame = 0


Query: 16  VVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSIA-FWIMNC------- 75
           VV+RK D+E+L    F+ND II+FY  YL          +SP     F   NC       
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKNR-------ISPKERGRFHFFNCFFFRKLA 364

Query: 76  -------------PDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYR 135
                           + +  + + ++L  K  +  P+N +          HWSL+    
Sbjct: 365 NLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSF---------HWSLVIICH 424

Query: 136 EANI----------------------FVHHDSNKGMNKYAAKRLYKAV------ARFMND 176
              +                       +H DS KG +K     ++ +       AR  N 
Sbjct: 425 PGELVPSHVNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENT 484

[Close]

BLAST of Chy11G198870 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 3.2e-05
Identity = 43/198 (21.72%), Postives = 76/198 (38.38%), Query Frame = 0


Query: 16  VVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSIAFWIMNCPDVES--- 75
           V + K D+E+L    F+ND II+FY +YL     + +        +F+     D++    
Sbjct: 426 VSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPS 485

Query: 76  -----------LNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANI--- 135
                      +  +   +++  K  +  PVN N+         HWSL+       +   
Sbjct: 486 SIADGKAAFLRVRKWTRKVDMFGKDYIFVPVNYNL---------HWSLIVICHPGEVANR 545

Query: 136 -------------FVHHDSNKG--------MNKYAAKRLYKAVARFMNDDSNSTSNPSYV 176
                         +H DS KG        +  Y  +   +      +D S+   N  +V
Sbjct: 546 TDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFV 605

[Close]

BLAST of Chy11G198870 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 3.2e-05
Identity = 43/198 (21.72%), Postives = 76/198 (38.38%), Query Frame = 0


Query: 16  VVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDVLLSPPSIAFWIMNCPDVES--- 75
           V + K D+E+L    F+ND II+FY +YL     + +        +F+     D++    
Sbjct: 394 VSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPS 453

Query: 76  -----------LNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANI--- 135
                      +  +   +++  K  +  PVN N+         HWSL+       +   
Sbjct: 454 SIADGKAAFLRVRKWTRKVDMFGKDYIFVPVNYNL---------HWSLIVICHPGEVANR 513

Query: 136 -------------FVHHDSNKG--------MNKYAAKRLYKAVARFMNDDSNSTSNPSYV 176
                         +H DS KG        +  Y  +   +      +D S+   N  +V
Sbjct: 514 TDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFV 573

The following BLAST results are available for this feature:

BLAST of Chy11G198870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSS7	1.3e-70	57.27	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
Q9D2Z4	5.5e-26	32.16	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
Q5FVJ8	5.5e-26	35.91	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
Q96LD8	1.2e-25	32.43	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
Q54XR2	1.4e-24	32.23	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Chy11G198870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KEM9	1.8e-128	95.76	ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G28972...	[more]
A0A5D3CSG4	2.6e-127	94.92	NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CK81	2.6e-127	94.92	NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1	[more]
A0A6J1CWB2	4.3e-106	78.81	NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV...	[more]
A0A6J1KYI9	2.7e-100	75.73	NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1	[more]

BLAST of Chy11G198870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149559.1	1.81e-165	95.76	NEDD8-specific protease 1 [Cucumis sativus] >XP_031742683.1 NEDD8-specific prote...	[more]
XP_008463889.1	6.05e-164	94.92	PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific...	[more]
XP_038902669.1	2.14e-148	84.75	NEDD8-specific protease 1 [Benincasa hispida]	[more]
XP_022146090.1	4.70e-136	78.81	NEDD8-specific protease 1 [Momordica charantia]	[more]
XP_023007377.1	2.09e-128	75.73	NEDD8-specific protease 1 [Cucurbita maxima]	[more]

BLAST of Chy11G198870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60190.1	8.9e-72	57.27	Cysteine proteinases superfamily protein	[more]
AT4G33620.1	3.8e-06	22.86	Cysteine proteinases superfamily protein	[more]
AT1G09730.1	3.2e-05	21.72	Cysteine proteinases superfamily protein	[more]
AT1G09730.2	3.2e-05	21.72	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 7..228 e-value: 6.9E-62 score: 211.0
None	No IPR available	PANTHER	PTHR46468:SF2	PROTEASE, SENP8, PUTATIVE-RELATED	coord: 7..156
None	No IPR available	PANTHER	PTHR46468:SF2	PROTEASE, SENP8, PUTATIVE-RELATED	coord: 161..225
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 31..180 e-value: 5.1E-13 score: 49.4
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 16..182 score: 13.904947
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 161..225
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 7..156
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 7..226

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy11G198870.1	Chy11G198870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0008234	cysteine-type peptidase activity