Chy11G193690 (gene) Cucumber (hystrix) v1

Overview
NameChy11G193690
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionSPARK domain-containing protein
LocationchrH11: 11181959 .. 11182972 (-)
RNA-Seq ExpressionChy11G193690
SyntenyChy11G193690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCTCCATACTTGGAATTGCAACACTTGTTATCTCAATGACTCTGTTCCCGATTTCCGTTGCTGTGTTCGTACGAGGGCAGTTCTCGGTTCCGGCCTTCCCAGTGGAAACCGAAAATCAAGCCTGCCGGTTGGACTTATCGGACGAGCTATTCGGGGGAGTGAGTCAAGCCTGCAATGGGAATCTAGACCGGAGCCGGTGCTGTCCAGTGCTAGCCGCATGGCTCTTCGCGGCTCACGCTAGGTCGGCGTTGGAAGTGTCGGCTCCAGCTCCATCAGCCGAAATAGATCTGCCATTGATGCCAGACGATTCCCAGAAATGCGTAGAATCACTTCAGAGCTCATTGGTGAGGCGCAACATCAGAATTCCCCAGCCGAATTCCAGCTGTGACGCCGTTTTGTGCTTCTGCGGAATTCGACTCCATCAGATAAGTTCCTTGTCTTGTCCTGCCGCATTCAATCTGTCCGGCTTTCAAAATGCATCCCCGACAGCGGCGGTTCGTGATTTGGAGACGAATTGTCGTAATGCTTCTTACTCTGGTTGCACTAAATGCCTTGGCGCACTTCAAAAGGTACAAAAAGAAAAAAAAACAACTAATCCAAACTTTTGTACAGTGGGTTACTGAAAATTGGGTTATTGGTTAAGAATTATGAATTGTATTGGGAATTAGGTAAAGGGGGCTGGAGATAGGAGCACGACGGCAAGAGCAAGAAAAATGTTCAACAGAGACTGCCAATTAATGGGGCTGACATGGTTGCTTGCTCGTAACAAAACGGCGTACATTCCGACGGTTTCGGCGGTTTTGAGGGCAATTATGTACAGTACCCATCCGCCTCACAATTCCATGTGCAGCCCAGATCAAGAGAACATGCCACTCGCTGTTGATTCTCTTCAATTTGATGACGCACATTCAATTTCACACTCACAACCCTCCTCAATTTTTATTTTTTCATTTTTGCGCTTAGTGCTAACTCCTTTTGTTTGCCTGTTCTTAGTAAAAATATGGTCGTAG

mRNA sequence

ATGATCTCCATACTTGGAATTGCAACACTTGTTATCTCAATGACTCTGTTCCCGATTTCCGTTGCTGTGTTCGTACGAGGGCAGTTCTCGGTTCCGGCCTTCCCAGTGGAAACCGAAAATCAAGCCTGCCGGTTGGACTTATCGGACGAGCTATTCGGGGGAGTGAGTCAAGCCTGCAATGGGAATCTAGACCGGAGCCGGTGCTGTCCAGTGCTAGCCGCATGGCTCTTCGCGGCTCACGCTAGGTCGGCGTTGGAAGTGTCGGCTCCAGCTCCATCAGCCGAAATAGATCTGCCATTGATGCCAGACGATTCCCAGAAATGCGTAGAATCACTTCAGAGCTCATTGGTGAGGCGCAACATCAGAATTCCCCAGCCGAATTCCAGCTGTGACGCCGTTTTGTGCTTCTGCGGAATTCGACTCCATCAGATAAGTTCCTTGTCTTGTCCTGCCGCATTCAATCTGTCCGGCTTTCAAAATGCATCCCCGACAGCGGCGGTTCGTGATTTGGAGACGAATTGTCGTAATGCTTCTTACTCTGGTTGCACTAAATGCCTTGGCGCACTTCAAAAGGTAAAGGGGGCTGGAGATAGGAGCACGACGGCAAGAGCAAGAAAAATGTTCAACAGAGACTGCCAATTAATGGGGCTGACATGGTTGCTTGCTCGTAACAAAACGGCGTACATTCCGACGGTTTCGGCGGTTTTGAGGGCAATTATGTACAGTACCCATCCGCCTCACAATTCCATGTGCAGCCCAGATCAAGAGAACATGCCACTCGCTGTTGATTCTCTTCAATTTGATGACGCACATTCAATTTCACACTCACAACCCTCCTCAATTTTTATTTTTTCATTTTTGCGCTTAGTGCTAACTCCTTTTGTTTGCCTGTTCTTAGTAAAAATATGGTCGTAG

Coding sequence (CDS)

ATGATCTCCATACTTGGAATTGCAACACTTGTTATCTCAATGACTCTGTTCCCGATTTCCGTTGCTGTGTTCGTACGAGGGCAGTTCTCGGTTCCGGCCTTCCCAGTGGAAACCGAAAATCAAGCCTGCCGGTTGGACTTATCGGACGAGCTATTCGGGGGAGTGAGTCAAGCCTGCAATGGGAATCTAGACCGGAGCCGGTGCTGTCCAGTGCTAGCCGCATGGCTCTTCGCGGCTCACGCTAGGTCGGCGTTGGAAGTGTCGGCTCCAGCTCCATCAGCCGAAATAGATCTGCCATTGATGCCAGACGATTCCCAGAAATGCGTAGAATCACTTCAGAGCTCATTGGTGAGGCGCAACATCAGAATTCCCCAGCCGAATTCCAGCTGTGACGCCGTTTTGTGCTTCTGCGGAATTCGACTCCATCAGATAAGTTCCTTGTCTTGTCCTGCCGCATTCAATCTGTCCGGCTTTCAAAATGCATCCCCGACAGCGGCGGTTCGTGATTTGGAGACGAATTGTCGTAATGCTTCTTACTCTGGTTGCACTAAATGCCTTGGCGCACTTCAAAAGGTAAAGGGGGCTGGAGATAGGAGCACGACGGCAAGAGCAAGAAAAATGTTCAACAGAGACTGCCAATTAATGGGGCTGACATGGTTGCTTGCTCGTAACAAAACGGCGTACATTCCGACGGTTTCGGCGGTTTTGAGGGCAATTATGTACAGTACCCATCCGCCTCACAATTCCATGTGCAGCCCAGATCAAGAGAACATGCCACTCGCTGTTGATTCTCTTCAATTTGATGACGCACATTCAATTTCACACTCACAACCCTCCTCAATTTTTATTTTTTCATTTTTGCGCTTAGTGCTAACTCCTTTTGTTTGCCTGTTCTTAGTAAAAATATGGTCGTAG

Protein sequence

MISILGIATLVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYSGCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFVCLFLVKIWS*
Homology
BLAST of Chy11G193690 vs. ExPASy Swiss-Prot
Match: Q9SUC9 (Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 GN=At4g28100 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.0e-105
Identity = 198/277 (71.48%), Postives = 231/277 (83.39%), Query Frame = 0

Query: 30  SVPAFPVETENQACRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSA 89
           +VPAFPVET+ Q+CRLDLS+ELFGGV++AC  NLDRSRCCPVLAAWLFAAHARSAL++ A
Sbjct: 33  TVPAFPVETQAQSCRLDLSNELFGGVNEACGRNLDRSRCCPVLAAWLFAAHARSALQLPA 92

Query: 90  PAP---SAEIDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNSSCDAVLCFCGIRLHQISS 149
           PAP   S++ D P+ PDDSQKCV +LQS+L+ + I+IPQPNSSCDA+LCFCGIRLHQISS
Sbjct: 93  PAPTPESSDPDEPMKPDDSQKCVNTLQSALLTKQIKIPQPNSSCDAILCFCGIRLHQISS 152

Query: 150 LSCPAAFNL-SGFQNASPTAAVRDLETNCRNASYSGCTKCLGALQ--KVKGAGDRSTTAR 209
           LSCPAAFN+ SGF+NA+PTAAV++LE  CRN+SYSGCT+CLGALQ  KV+G   ++TT R
Sbjct: 153 LSCPAAFNVSSGFKNATPTAAVKNLEKECRNSSYSGCTRCLGALQKLKVRGGNKKTTTER 212

Query: 210 ARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSTHPPHNSMCSPDQENMPLAVD 269
             KM ++DCQLMGLTWLLARNKTAYIPTVSAVLRAIMYS HPPH + CSPDQENMPLAVD
Sbjct: 213 GTKMMSKDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVD 272

Query: 270 SLQFDDAHSISHSQPSSIF-IFSFLRLVLTPFVCLFL 300
           SLQF      S S  S +F +  FL LVL    C+FL
Sbjct: 273 SLQFQK----SFSSSSHLFGVLPFLPLVL----CIFL 301

BLAST of Chy11G193690 vs. ExPASy TrEMBL
Match: A0A0A0KAY3 (SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G121950 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 297/303 (98.02%), Postives = 299/303 (98.68%), Query Frame = 0

Query: 1   MISILGIATLVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60
           MISIL IATLVIS TLFPISVAV VRGQ+SVPAFPVETENQACRLDLSDELFGGVSQACN
Sbjct: 1   MISILAIATLVISTTLFPISVAVSVRGQYSVPAFPVETENQACRLDLSDELFGGVSQACN 60

Query: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120
           GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN
Sbjct: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120

Query: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS 180
           IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS
Sbjct: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS 180

Query: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240
           GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM
Sbjct: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240

Query: 241 YSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFVCLFLV 300
           YS HPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRL+LTPFVCLFLV
Sbjct: 241 YSAHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLLLTPFVCLFLV 300

Query: 301 KIW 304
           KIW
Sbjct: 301 KIW 303

BLAST of Chy11G193690 vs. ExPASy TrEMBL
Match: A0A5D3B9U0 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00450 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.7e-160
Identity = 292/303 (96.37%), Postives = 294/303 (97.03%), Query Frame = 0

Query: 1   MISILGIATLVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60
           MISIL IAT +IS TLFPISVAV VRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN
Sbjct: 1   MISILAIATFLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60

Query: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120
           GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN
Sbjct: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120

Query: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS 180
           IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPT AVRDLETNCRNASYS
Sbjct: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNASYS 180

Query: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240
           GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM
Sbjct: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240

Query: 241 YSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFVCLFLV 300
           YS H PHNSMCSPDQENMPLAVDSLQFDDAHSISHSQP SIF+FSF RLVLTPFVCLFLV
Sbjct: 241 YSAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQP-SIFMFSFFRLVLTPFVCLFLV 300

Query: 301 KIW 304
           KIW
Sbjct: 301 KIW 302

BLAST of Chy11G193690 vs. ExPASy TrEMBL
Match: A0A1S3BJT3 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=LOC103490480 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.7e-160
Identity = 292/303 (96.37%), Postives = 294/303 (97.03%), Query Frame = 0

Query: 1   MISILGIATLVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60
           MISIL IAT +IS TLFPISVAV VRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN
Sbjct: 1   MISILAIATFLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60

Query: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120
           GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN
Sbjct: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120

Query: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS 180
           IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPT AVRDLETNCRNASYS
Sbjct: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNASYS 180

Query: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240
           GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM
Sbjct: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240

Query: 241 YSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFVCLFLV 300
           YS H PHNSMCSPDQENMPLAVDSLQFDDAHSISHSQP SIF+FSF RLVLTPFVCLFLV
Sbjct: 241 YSAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQP-SIFMFSFFRLVLTPFVCLFLV 300

Query: 301 KIW 304
           KIW
Sbjct: 301 KIW 302

BLAST of Chy11G193690 vs. ExPASy TrEMBL
Match: A0A6J1G647 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=3662 GN=LOC111451182 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 7.9e-129
Identity = 243/297 (81.82%), Postives = 263/297 (88.55%), Query Frame = 0

Query: 1   MISILGIAT--LVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQA 60
           M+S++ + +  L+I  TLF +SVA  V G+ SVPA PVE E Q CRLDLSDELFGGVSQA
Sbjct: 106 MVSLMVVVSSILMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQA 165

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVR 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SLV+
Sbjct: 166 CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 225

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+PT+AVRDLE NCRNAS
Sbjct: 226 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNAS 285

Query: 181 YSGCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240
           YSGCTKCLGALQKVKGA D STT RA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA
Sbjct: 286 YSGCTKCLGALQKVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 345

Query: 241 IMYSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFV 296
           IMYS HPPH+S CSPDQENMPLAVDSLQF++  S S S PS+ F++ FL L   PF+
Sbjct: 346 IMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQS-STSSPST-FMYPFLPLTAVPFI 399

BLAST of Chy11G193690 vs. ExPASy TrEMBL
Match: A0A6J1HZY7 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 GN=LOC111469629 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.3e-127
Identity = 241/297 (81.14%), Postives = 261/297 (87.88%), Query Frame = 0

Query: 1   MISILGIAT--LVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQA 60
           M+S++ + +  L+I  TLF +SVA  V G+ SVPA PVE E Q CRLDLSDELFGGVSQA
Sbjct: 1   MVSLMVVVSSILMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQA 60

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVR 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SLV+
Sbjct: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 120

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+PT AVRDLE NCRNAS
Sbjct: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTPAVRDLEKNCRNAS 180

Query: 181 YSGCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240
           YSGCTKCLGALQKVKGA D +TT RA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA
Sbjct: 181 YSGCTKCLGALQKVKGARDTNTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240

Query: 241 IMYSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFV 296
           IMYS HPPH+S CSPDQENMPLAVDSLQF++  S S S PS+ F++ FL L    F+
Sbjct: 241 IMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQS-STSSPST-FMYPFLPLTAVLFI 294

BLAST of Chy11G193690 vs. NCBI nr
Match: XP_004139964.1 (uncharacterized GPI-anchored protein At4g28100 [Cucumis sativus] >KGN46678.1 hypothetical protein Csa_020848 [Cucumis sativus])

HSP 1 Score: 582 bits (1501), Expect = 3.16e-209
Identity = 297/303 (98.02%), Postives = 299/303 (98.68%), Query Frame = 0

Query: 1   MISILGIATLVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60
           MISIL IATLVIS TLFPISVAV VRGQ+SVPAFPVETENQACRLDLSDELFGGVSQACN
Sbjct: 1   MISILAIATLVISTTLFPISVAVSVRGQYSVPAFPVETENQACRLDLSDELFGGVSQACN 60

Query: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120
           GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN
Sbjct: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120

Query: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS 180
           IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS
Sbjct: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS 180

Query: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240
           GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM
Sbjct: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240

Query: 241 YSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFVCLFLV 300
           YS HPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRL+LTPFVCLFLV
Sbjct: 241 YSAHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLLLTPFVCLFLV 300

Query: 301 KIW 303
           KIW
Sbjct: 301 KIW 303

BLAST of Chy11G193690 vs. NCBI nr
Match: XP_008448226.1 (PREDICTED: uncharacterized GPI-anchored protein At4g28100-like [Cucumis melo] >TYJ96632.1 putative GPI-anchored protein [Cucumis melo var. makuwa])

HSP 1 Score: 568 bits (1464), Expect = 1.33e-203
Identity = 292/303 (96.37%), Postives = 294/303 (97.03%), Query Frame = 0

Query: 1   MISILGIATLVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60
           MISIL IAT +IS TLFPISVAV VRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN
Sbjct: 1   MISILAIATFLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACN 60

Query: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120
           GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN
Sbjct: 61  GNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRN 120

Query: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYS 180
           IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPT AVRDLETNCRNASYS
Sbjct: 121 IRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNASYS 180

Query: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240
           GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM
Sbjct: 181 GCTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIM 240

Query: 241 YSTHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFVCLFLV 300
           YS H PHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPS IF+FSF RLVLTPFVCLFLV
Sbjct: 241 YSAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPS-IFMFSFFRLVLTPFVCLFLV 300

Query: 301 KIW 303
           KIW
Sbjct: 301 KIW 302

BLAST of Chy11G193690 vs. NCBI nr
Match: XP_038901814.1 (uncharacterized GPI-anchored protein At4g28100-like [Benincasa hispida])

HSP 1 Score: 511 bits (1315), Expect = 6.48e-181
Identity = 263/294 (89.46%), Postives = 270/294 (91.84%), Query Frame = 0

Query: 10  LVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACNGNLDRSRCC 69
           L+IS TLFPISVAV VRGQFSVPAFPVETE Q CRLDLSDELFGGVSQACNGNLDRSRCC
Sbjct: 12  LLISTTLFPISVAVLVRGQFSVPAFPVETETQTCRLDLSDELFGGVSQACNGNLDRSRCC 71

Query: 70  PVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNSS 129
           PVLAAWLFAAHARSALEVSAP PSAEIDLPLMPDDSQKCVESLQSSL RRNI+IPQPNSS
Sbjct: 72  PVLAAWLFAAHARSALEVSAPTPSAEIDLPLMPDDSQKCVESLQSSLARRNIKIPQPNSS 131

Query: 130 CDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYSGCTKCLGAL 189
           CDAVLCFCGIRLHQISSLSCPAAFNLSGFQNA+PTAAVRDLE NC+NASYSGCTKCLGAL
Sbjct: 132 CDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTAAVRDLEKNCQNASYSGCTKCLGAL 191

Query: 190 QKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSTHPPHNS 249
           QKVKG  D STT RARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYS HPPHNS
Sbjct: 192 QKVKGVRDSSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHNS 251

Query: 250 MCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFVCLFLVKIW 303
            CSPDQENMPLAVD LQFDDAHS S S   SIF+  FLRL+LTP VCL LV +W
Sbjct: 252 KCSPDQENMPLAVDYLQFDDAHSQSQS---SIFMLPFLRLLLTPLVCL-LVIVW 301

BLAST of Chy11G193690 vs. NCBI nr
Match: KAG7034939.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466 bits (1200), Expect = 1.58e-163
Identity = 241/286 (84.27%), Postives = 256/286 (89.51%), Query Frame = 0

Query: 10  LVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACNGNLDRSRCC 69
           L+I  TLF +SVA  V G+ SVPA PVE E Q CRLDLSDELFGGVSQACNGNLDRSRCC
Sbjct: 12  LMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDRSRCC 71

Query: 70  PVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNSS 129
           PVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SLV+RNIRIPQPNSS
Sbjct: 72  PVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSS 131

Query: 130 CDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYSGCTKCLGAL 189
           CDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+PT+AVRDLE NCRNASYSGCTKCLGAL
Sbjct: 132 CDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASYSGCTKCLGAL 191

Query: 190 QKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSTHPPHNS 249
           QKVKGA D STT RA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYS HPPH+S
Sbjct: 192 QKVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHDS 251

Query: 250 MCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFV 295
            CSPDQENMPLAVDSLQF++  S S S PS+ F++ FL L   PF+
Sbjct: 252 KCSPDQENMPLAVDSLQFENTQS-STSSPST-FMYPFLPLTAVPFI 294

BLAST of Chy11G193690 vs. NCBI nr
Match: XP_023532424.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 465 bits (1196), Expect = 7.98e-163
Identity = 241/286 (84.27%), Postives = 254/286 (88.81%), Query Frame = 0

Query: 10  LVISMTLFPISVAVFVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACNGNLDRSRCC 69
           L+I  TLF +SVA  V G+ SVPA PVE E Q CRLDLSDELFGGVSQACNGNLDRSRCC
Sbjct: 12  LMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDRSRCC 71

Query: 70  PVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNSS 129
           PVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SLV+RNIRIPQPNSS
Sbjct: 72  PVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSS 131

Query: 130 CDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYSGCTKCLGAL 189
           CDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+PT AVRDLE NCRNASYSGCTKCLGAL
Sbjct: 132 CDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTPAVRDLEKNCRNASYSGCTKCLGAL 191

Query: 190 QKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSTHPPHNS 249
           QKVKGA D STT RA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYS HPPH+S
Sbjct: 192 QKVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHDS 251

Query: 250 MCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLVLTPFV 295
            CSPDQENMPLAVDSLQ D+  S S S PS+ F++ FL L   PF+
Sbjct: 252 KCSPDQENMPLAVDSLQLDNTQS-STSSPST-FMYPFLPLTAVPFI 294

BLAST of Chy11G193690 vs. TAIR 10
Match: AT4G28100.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G18050.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 383.6 bits (984), Expect = 1.4e-106
Identity = 198/277 (71.48%), Postives = 231/277 (83.39%), Query Frame = 0

Query: 30  SVPAFPVETENQACRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSA 89
           +VPAFPVET+ Q+CRLDLS+ELFGGV++AC  NLDRSRCCPVLAAWLFAAHARSAL++ A
Sbjct: 33  TVPAFPVETQAQSCRLDLSNELFGGVNEACGRNLDRSRCCPVLAAWLFAAHARSALQLPA 92

Query: 90  PAP---SAEIDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNSSCDAVLCFCGIRLHQISS 149
           PAP   S++ D P+ PDDSQKCV +LQS+L+ + I+IPQPNSSCDA+LCFCGIRLHQISS
Sbjct: 93  PAPTPESSDPDEPMKPDDSQKCVNTLQSALLTKQIKIPQPNSSCDAILCFCGIRLHQISS 152

Query: 150 LSCPAAFNL-SGFQNASPTAAVRDLETNCRNASYSGCTKCLGALQ--KVKGAGDRSTTAR 209
           LSCPAAFN+ SGF+NA+PTAAV++LE  CRN+SYSGCT+CLGALQ  KV+G   ++TT R
Sbjct: 153 LSCPAAFNVSSGFKNATPTAAVKNLEKECRNSSYSGCTRCLGALQKLKVRGGNKKTTTER 212

Query: 210 ARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSTHPPHNSMCSPDQENMPLAVD 269
             KM ++DCQLMGLTWLLARNKTAYIPTVSAVLRAIMYS HPPH + CSPDQENMPLAVD
Sbjct: 213 GTKMMSKDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVD 272

Query: 270 SLQFDDAHSISHSQPSSIF-IFSFLRLVLTPFVCLFL 300
           SLQF      S S  S +F +  FL LVL    C+FL
Sbjct: 273 SLQFQK----SFSSSSHLFGVLPFLPLVL----CIFL 301

BLAST of Chy11G193690 vs. TAIR 10
Match: AT3G18050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G28100.1); Has 67 Blast hits to 66 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 67; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 198.7 bits (504), Expect = 6.5e-51
Identity = 126/332 (37.95%), Postives = 188/332 (56.63%), Query Frame = 0

Query: 4   ILGIATLVISMTLFPISVAVF-VRGQ--FSVPAFPVETENQACRLDLSDELFGGVSQACN 63
           I+ + T++  ++  P++V  F V+     ++PAFP +++   C LDL ++LF G+  AC+
Sbjct: 13  IIFLFTILSPVSPEPVTVQPFRVKSSPPATIPAFPEQSDFSGCPLDLPEDLFHGIKSACS 72

Query: 64  G-NLDRSRCCPVLAAWLFAAH------------ARSALEVSAPAPSAEIDLPLMPDDSQK 123
           G  L + +CCPVL AWL++A+            AR++   SA   + E D+PL+PDDS+ 
Sbjct: 73  GKKLHKGKCCPVLGAWLYSAYSTTALSRSISAAARNSSSSSAAVTTPEEDMPLLPDDSET 132

Query: 124 CVESLQSSLVRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAV 183
           CV+ L  SL +R I + +PN +CD V C+CGIRLH    LSC  AF ++          V
Sbjct: 133 CVDGLGKSLRQRGIELTRPNETCDVVYCYCGIRLH---PLSCSEAFRVNDEGRLVGDERV 192

Query: 184 RDLETNCRNASYS---------GCTKCLGALQKV---KGAGDRSTTA----RARKMFNRD 243
             LET+C + S++         GC KCL +L K+   K +G R+ +     R  KM N+D
Sbjct: 193 DRLETDCLSGSHNNADGFSPLLGCNKCLNSLYKLNPKKTSGTRNPSKEDRNRTAKMHNKD 252

Query: 244 CQLMGLTWLLARNKTAYIPTVSAVLRAIMYSTHPPHNSMCSPDQENMPLAVDSLQFDDAH 303
           C LMGLTWLLA+N+TAY PTV++VLRA+M + H      C+   + MPLAVDS +F +  
Sbjct: 253 CVLMGLTWLLAKNRTAYFPTVTSVLRAVMLN-HDGVPRSCALGSDGMPLAVDSSEFSNGS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUC92.0e-10571.48Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A0A0KAY34.4e-16498.02SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G121950 PE=4 ... [more]
A0A5D3B9U08.7e-16096.37Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BJT38.7e-16096.37uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=L... [more]
A0A6J1G6477.9e-12981.82uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=366... [more]
A0A6J1HZY74.3e-12781.14uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
XP_004139964.13.16e-20998.02uncharacterized GPI-anchored protein At4g28100 [Cucumis sativus] >KGN46678.1 hyp... [more]
XP_008448226.11.33e-20396.37PREDICTED: uncharacterized GPI-anchored protein At4g28100-like [Cucumis melo] >T... [more]
XP_038901814.16.48e-18189.46uncharacterized GPI-anchored protein At4g28100-like [Benincasa hispida][more]
KAG7034939.11.58e-16384.27putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_023532424.17.98e-16384.27uncharacterized GPI-anchored protein At4g28100-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G28100.11.4e-10671.48unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G18050.16.5e-5137.95unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043891SPARK domainPFAMPF19160SPARKcoord: 39..194
e-value: 1.2E-17
score: 64.8
IPR040376At4g28100-likePANTHERPTHR34056GPI-ANCHORED PROTEINcoord: 28..295
NoneNo IPR availablePANTHERPTHR34056:SF3OS07G0557700 PROTEINcoord: 28..295

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G193690.1Chy11G193690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane