Chy11G191610 (gene) Cucumber (hystrix) v1

Overview


Name	Chy11G191610
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	cytochrome P450 81D1-like
Location	chrH11: 6640027 .. 6642396 (+)
RNA-Seq Expression	Chy11G191610
Synteny	Chy11G191610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTTGATCACTTGCTTTACGCTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCACCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTTCATCTTCTCAAACATCCTGTCCACAAAACATTACAAACCCTCTCCCAAAAGTATGGCCACGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCCGCCGTCCAAGAATGTTTCACAAAGAACGATATCATTCTTGCAAATCGACCATCTTTAAACTTCGGAAAGTACTTATCATACAACAATACCACCATGGTGGTGTCCCCTTATGGTGAACATTGGCGAAACCTCCGCAGAATTTGTACCCTCGAAATTTTCTCGACAACTCGTCTTAATTCGTTCTCGAAAATTCGAGAGGAGGAAGTCAGACGTTTACTATGTAAATTATGTGGTAATTATAAGTTGGAAGATGAGTTTAGGGTTGTGGAATTGGAGTCAATGTTGTTGGATCTTACATTCAATATTGTCATGAGAATGGTGGGTGGAAAGAAGTTTTGCAAAGAGAATAATAAAAATAATAATGTGTTGGAATATGAGGGATATTGCAAAAGATTTAAAGAGTTGGTGACACAAATAATGGCACATGCTGGATCAACAAATCCAGGGGATTTCATACCTTTATGGAATTGGATTGATCCAAGTGGTTATAATAAAAGGATAATGAAGATTGGAAGAAGAATGGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATTCGAAATATTATAGTAACCTAATTATCAAAGGTCTCATACAAGTATGTTTTTCTTTCTAAATTAGAACATCTAAGTTTTATTTTCCAATATATAAACATTTTTTTGAGTAACAATATGATCTTTTTATTTTTTATTTTACACTTTAGAGATTCAAGCTTCTAATTACTTGGTTTTTTGTTTTAAAAATTTTGCTTGTTTCTCACTATTTACCGCTTAGAGTTTTCAAATTTTCAATTTAAGCATTTGAATTCTTAGTCAAATATTTAAAAACAAAAACTACTTTTTTTACTGTCTCTTTTCGTAACTATTTGGTTTATATATAACTTTTAAAACAAAAATTAAATTTATAGACATGGCTTCCATCTCCCAAATTTCTTGATTTGTTACAAACTTTTTACGAGTGAATTAAATAACCAAGCCAATTTTCAAGAGAAAGTAGTTTTTAAGAATTTCTTATTGTTTTTGGAATTTAACTAGAAATTCAACCATTGTACTTAAAAAAACGCAAATTATTGTAAGAAATTGAGAGGAAATTAATATACTTAATTAATTTTCAAAGACCAAAAACTAAAATTAAAATAGTTACCAAACGAGACCTTAATTTTGAAAACTTGATTTTGATTCTGGAAACACCCAACAACGAAACATATAAATTAATAGAGATAATGAGTGTTTATAAACAATTTTGAGAAACTAGAAGTAAAAACCAAGCAAGTGCATTAAATTCTGAATTTTCAAATTTTGGTAACAATTTAGCTTCTATATATTTTATTGATTGATAAATTAATAAGAAATCAAAACCTTTTTTAAACTATATTTGAAGAGTAAGTTTGTACGTGGTGAAAGTTGACAGTTAATTTTGATGATCAAATTTTTCATAGAGACTAAAATGGTTAGAAATATATAGCAAATGACTTCTCTTTTTTCCAACCAAAAATACACCATAACATTAAAGCTAACTTAGATGCATTCCCTTTCAAGCTCATGATTCGCCCCCTTGTGTAACCAAATGTAAGATCACTTTGATCTAATTACAAAAAACAAACTAAAGTTGGTGTGCGTGGATCGTTTTTCTCAAGAATATATGCATGGATTCACCTAATGCTCAATTATTCTTGCAGGATATATTAATAGCAGGGATTGACACAAGCGCTGTGACATTACAATGGGCGCTATCTCATTTACTTAACAATCCAATCGTCTTAGATAAGGCCAAAGCCGAAATAGATTCCTATATAGGACAAGAACGTATGGTGAATGAAGTCGATTTATCGAGTTTAAGTTACCTACAAGGAATAATCTCCGAGACTCTTCGGCTAAGTCCAGCTGGTCCTTTACTCGTACCACATTGTGCATCTGAAGACTGCAAAATAGGAGGTTATGACGTTCCACGTAATACAATCGTACTGATTAATGCTTGGGCTATACATCGAGATCTAAATCTATGGGAAGATGCTAGTAGTTTTAAAGCCAGAAAGACATGTAAATGCGGTTGGATTTGA

mRNA sequence

ATGGATTTTGATCACTTGCTTTACGCTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCACCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTTCATCTTCTCAAACATCCTGTCCACAAAACATTACAAACCCTCTCCCAAAAGTATGGCCACGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCCGCCGTCCAAGAATGTTTCACAAAGAACGATATCATTCTTGCAAATCGACCATCTTTAAACTTCGGAAAGTACTTATCATACAACAATACCACCATGGTGGTGTCCCCTTATGGTGAACATTGGCGAAACCTCCGCAGAATTTGTACCCTCGAAATTTTCTCGACAACTCGTCTTAATTCGTTCTCGAAAATTCGAGAGGAGGAAGTCAGACGTTTACTATGTAAATTATGTGGTAATTATAAGTTGGAAGATGAGTTTAGGGTTGTGGAATTGGAGTCAATGTTGTTGGATCTTACATTCAATATTGTCATGAGAATGGTGGGTGGAAAGAAGTTTTGCAAAGAGAATAATAAAAATAATAATGTGTTGGAATATGAGGGATATTGCAAAAGATTTAAAGAGTTGGTGACACAAATAATGGCACATGCTGGATCAACAAATCCAGGGGATTTCATACCTTTATGGAATTGGATTGATCCAAGTGGTTATAATAAAAGGATAATGAAGATTGGAAGAAGAATGGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATTCGAAATATTATAGTAACCTAATTATCAAAGGTCTCATACAAGATATATTAATAGCAGGGATTGACACAAGCGCTGTGACATTACAATGGGCGCTATCTCATTTACTTAACAATCCAATCGTCTTAGATAAGGCCAAAGCCGAAATAGATTCCTATATAGGACAAGAACGTATGGTGAATGAAGTCGATTTATCGAGTTTAAGTTACCTACAAGGAATAATCTCCGAGACTCTTCGGCTAAGTCCAGCTGGTCCTTTACTCGTACCACATTGTGCATCTGAAGACTGCAAAATAGGAGGTTATGACGTTCCACGTAATACAATCGTACTGATTAATGCTTGGGCTATACATCGAGATCTAAATCTATGGGAAGATGCTAGTAGTTTTAAAGCCAGAAAGACATGTAAATGCGGTTGGATTTGA

Coding sequence (CDS)

ATGGATTTTGATCACTTGCTTTACGCTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCACCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTTCATCTTCTCAAACATCCTGTCCACAAAACATTACAAACCCTCTCCCAAAAGTATGGCCACGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCCGCCGTCCAAGAATGTTTCACAAAGAACGATATCATTCTTGCAAATCGACCATCTTTAAACTTCGGAAAGTACTTATCATACAACAATACCACCATGGTGGTGTCCCCTTATGGTGAACATTGGCGAAACCTCCGCAGAATTTGTACCCTCGAAATTTTCTCGACAACTCGTCTTAATTCGTTCTCGAAAATTCGAGAGGAGGAAGTCAGACGTTTACTATGTAAATTATGTGGTAATTATAAGTTGGAAGATGAGTTTAGGGTTGTGGAATTGGAGTCAATGTTGTTGGATCTTACATTCAATATTGTCATGAGAATGGTGGGTGGAAAGAAGTTTTGCAAAGAGAATAATAAAAATAATAATGTGTTGGAATATGAGGGATATTGCAAAAGATTTAAAGAGTTGGTGACACAAATAATGGCACATGCTGGATCAACAAATCCAGGGGATTTCATACCTTTATGGAATTGGATTGATCCAAGTGGTTATAATAAAAGGATAATGAAGATTGGAAGAAGAATGGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATTCGAAATATTATAGTAACCTAATTATCAAAGGTCTCATACAAGATATATTAATAGCAGGGATTGACACAAGCGCTGTGACATTACAATGGGCGCTATCTCATTTACTTAACAATCCAATCGTCTTAGATAAGGCCAAAGCCGAAATAGATTCCTATATAGGACAAGAACGTATGGTGAATGAAGTCGATTTATCGAGTTTAAGTTACCTACAAGGAATAATCTCCGAGACTCTTCGGCTAAGTCCAGCTGGTCCTTTACTCGTACCACATTGTGCATCTGAAGACTGCAAAATAGGAGGTTATGACGTTCCACGTAATACAATCGTACTGATTAATGCTTGGGCTATACATCGAGATCTAAATCTATGGGAAGATGCTAGTAGTTTTAAAGCCAGAAAGACATGTAAATGCGGTTGGATTTGA

Protein sequence

MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPYGEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLDLTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNWIDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKARKTCKCGWI*

Homology

[Close]

BLAST of Chy11G191610 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 7.7e-119
Identity = 222/426 (52.11%), Postives = 298/426 (69.95%), Query Frame = 0


Query: 6   LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
           +LY++ SLI L++ + FL KP + NLPP+P   LP IGHL LLK P+H+TL++ S+   H
Sbjct: 9   VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68

Query: 66  -----VFSLKFGSRLVVLVSSPS-AVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSP 125
                V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT M+ +P
Sbjct: 69  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128

Query: 126 YGEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRL---LCKLCGNYKLEDEFRVVELES 185
           YG+HWRNLRR+CT+EIFST RLN F  +R +EVRRL   L +L G  K      VVEL+ 
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK-----TVVELKP 188

Query: 186 MLLDLTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIP 245
           ML+DLTFN +MRM+ GK++  E   +      E   KR ++LV  + A+  S N  D++P
Sbjct: 189 MLMDLTFNNIMRMMTGKRYYGEETTD------EEEAKRVRKLVADVGANTSSGNAVDYVP 248

Query: 246 LWNWIDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNL 305
           +      S Y  R+ K+G   D+ LQGL+D+ R +++ G TMI HLL LQK+D +YY++ 
Sbjct: 249 ILRLF--SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQ 308

Query: 306 IIKGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSL 365
           IIKG+I  ++IAG +TSAVTL+WALS+LLN+P V+ KA+ EID+ +G +R++ E DLS L
Sbjct: 309 IIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSEL 368

Query: 366 SYLQGIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDAS 423
            YL+ I+ ETLRL PA PLLVPH ASEDCKIG YD+PR T +L+NAWAIHRD N W+D  
Sbjct: 369 PYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPD 420

[Close]

BLAST of Chy11G191610 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 6.1e-116
Identity = 210/423 (49.65%), Postives = 290/423 (68.56%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYS-FLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTL 60
           M+   LLY  L+++ L +    F     R NLPP+P   LP IGHLHL+   +H++L  L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  SQKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSP 120
           S+KYG VFSL+ G+RLV++VSSP+A +ECFTKNDI+ ANRP    GKY+ YN TTMV SP
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLL 180
           YGEHWRNLRR+  +EIFS   LN F  IRE+EV++LL  L        +F  VE++S L 
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSL--YQSSGQDFGKVEMKSKLS 180

Query: 181 DLTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWN 240
           +L+FN+ MRMV GK++  ++  ++         K F+ L+ ++  HAG++NPGDF+P   
Sbjct: 181 ELSFNVTMRMVAGKRYFGQDVDSDE-------AKLFRALIGEVFEHAGASNPGDFVPFLR 240

Query: 241 WIDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIK 300
           WID   Y K++ KI + MD  LQ L+ E R  ++   TMI HLL LQ++  +YY++ IIK
Sbjct: 241 WIDFKNYEKKVSKISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIK 300

Query: 301 GLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYL 360
           G+I  +L+AG DTSAVT++WA+S LLN+P  L+KA+ EI++ +G  R++ E DL  L+YL
Sbjct: 301 GIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYL 360

Query: 361 QGIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFK 420
             IISET RL PA P+LVPH +S+DCK+ GYDVP+ TI+L+NAWAIHRD   W++ + FK
Sbjct: 361 HNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFK 413

Query: 421 ARK 423
             +
Sbjct: 421 PER 413

[Close]

BLAST of Chy11G191610 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 8.8e-115
Identity = 214/423 (50.59%), Postives = 290/423 (68.56%), Query Frame = 0


Query: 7   LYALLSLISLLLGYSFLFKPHR--LNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYG 66
           L  ++SL  L++     F   R   NLPP P   LP IG+LH LK P+H T  TLSQKYG
Sbjct: 6   LSLIISLFFLIITLKVFFNTSRKFKNLPPGPQC-LPIIGNLHQLKQPLHHTFHTLSQKYG 65

Query: 67  HVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPYGEHW 126
            +FSL FGSRLVV+VSS +  QECFTKNDI+LANRP    GKY+ YNNTT+  SPYG+HW
Sbjct: 66  QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 125

Query: 127 RNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLDLTFN 186
           RNLRRI ++EI S+ RLNSF +IR +E+ RL+ KL    K  + F  VEL  M  ++TFN
Sbjct: 126 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLA--QKSYNGFTEVELRPMFSEMTFN 185

Query: 187 IVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNWIDPS 246
            +MRMV GK++   +   ++V E     + F+ ++ ++++  G+ N GDF+    W D  
Sbjct: 186 TIMRMVSGKRYYGNDCDVSDVEE----ARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD 245

Query: 247 GYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKGLIQD 306
           G  KR+ KI +R D  LQGL+DE R  +   NTMI HLL  Q++  +YY++ IIKGL+  
Sbjct: 246 GLEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVV 305

Query: 307 ILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGIIS 366
           +L+AG DTS+VT++WA+S+LLN+P ++ KAK E+D++IG +R V+E D+S L YLQ I+ 
Sbjct: 306 MLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVY 365

Query: 367 ETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKARKTC 426
           ETLRL  A PLLVPH +SED  +GGY++P+NTI+++NAW IHRD NLW D + FK  +  
Sbjct: 366 ETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFE 421

Query: 427 KCG 428
           K G
Sbjct: 426 KEG 421

[Close]

BLAST of Chy11G191610 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 6.3e-113
Identity = 216/421 (51.31%), Postives = 290/421 (68.88%), Query Frame = 0


Query: 8   YALLSLISLLLGYSFLFK--PHRL-NLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYG 67
           Y+LLSL S ++    L K    RL NLPP P   +P IG+LH LKHP+H+T  TLSQ YG
Sbjct: 5   YSLLSL-SFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYG 64

Query: 68  HVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPYGEHW 127
            +FSL FGSRLVV+VSSPS   ECFTKNDIILANRP    GKY+ YN TT+  + YG+HW
Sbjct: 65  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 124

Query: 128 RNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLED-EFRVVELESMLLDLTF 187
           RNLRRI T+++ S  RLNSF  +R +E  RL+ KL  +   E   F  VEL   L ++TF
Sbjct: 125 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 184

Query: 188 NIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNWIDP 247
           N +MRM+ GK++  ++   ++V E     K+F+E+++++M+  G+ N GDF+PL   +D 
Sbjct: 185 NAMMRMISGKRYYGDDGDVSDVEE----AKQFREIISEMMSLLGANNKGDFLPLLRVVDL 244

Query: 248 SGYNKRIMKIGRRMDEVLQGLVDEIR--NEEDEGNTMIQHLLRLQKTDSKYYSNLIIKGL 307
               KR  +I +R +  L+GL++E R  N   +G TMI HLL+L ++  +YYS+ +IKGL
Sbjct: 245 DNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGL 304

Query: 308 IQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQG 367
           IQ +L+AG DTSAVT++W +S LLN+P VL KAK E+D+ IG+ ++V+E DLS L YLQ 
Sbjct: 305 IQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQN 364

Query: 368 IISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKAR 423
           IISETLRL P  PLL+PH +SEDC IG ++VP++TI+L N W IHRD   W DA SFK  
Sbjct: 365 IISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPE 419

[Close]

BLAST of Chy11G191610 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 5.4e-112
Identity = 210/427 (49.18%), Postives = 290/427 (67.92%), Query Frame = 0


Query: 7   LYALLSL--ISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQK 66
           +Y +LSL    + L    LF  +P +LNLPP+P    P IGHLHLLK P+H+   +LS+ 
Sbjct: 11  IYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSES 70

Query: 67  YGH--VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 126
             +  +FSL  GSRLV +VSS +  +ECFTKND++LANRP    GK++ YN+TTMV + Y
Sbjct: 71  LNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAY 130

Query: 127 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 186
           G+ WRNLRRI T+EIFS+ RLNSF  IR++E+RRL+  L  N   +  F  VE++ + + 
Sbjct: 131 GDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKN--SQHGFVKVEMKPLFMC 190

Query: 187 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 246
           LT N ++RMV GK+F  +  +N+N        K  ++L+ +++   G+ N  D+ P+  +
Sbjct: 191 LTINNIIRMVAGKRFYGDGTENDN------EAKHVRQLIAEVVVSGGAGNAADYFPILRY 250

Query: 247 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 306
           +  + Y K + K+  R+DE LQ LV+E R E+ +GNTMI HLL LQ+T   YY+++IIKG
Sbjct: 251 V--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKG 310

Query: 307 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 366
           +I  +++AG DTSA TL+WA+S+LLN+P VL KAK EID  IG +R+V E D+  L YLQ
Sbjct: 311 IILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQ 370

Query: 367 GIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKA 426
            I+SETLRL P  P+L+PH ASEDC + GYDVPR TI+L+NAWAIHRD  LWE+   FK 
Sbjct: 371 HIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKP 427

Query: 427 RKTCKCG 428
            +  K G
Sbjct: 431 ERFEKKG 427

[Close]

BLAST of Chy11G191610 vs. ExPASy TrEMBL
Match: A0A0A0LQI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.1e-232
Identity = 402/422 (95.26%), Postives = 408/422 (96.68%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRLV+LVSSPSAVQECFTKNDIILANRPSLN GKYLSYNNTTM VSPY
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           GEHWRNLRRICTLEIFSTTRLNSFS+IREEEV+RLLCKLCGNYK EDEFRVVELESMLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LT NIVMRMVGGKKFCKENNKN NVLE EGYCKR KELVTQIMAHAGSTNPGDFIPLWNW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
           IDPSGYNKRIMKIGRRMDEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTD KYYS+LIIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKA 420
           GIISETLRLSP GPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRD N+WEDA SFK 
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420

Query: 421 RK 423
            +
Sbjct: 421 ER 422

[Close]

BLAST of Chy11G191610 vs. ExPASy TrEMBL
Match: A0A5A7SPU0 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00500 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 1.9e-221
Identity = 387/422 (91.71%), Postives = 400/422 (94.79%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLN GKYL+YNNTTMVVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           GEHWRNLRRI TLEIFSTTRLNSFS+ REEEV+RLL KL GNYKLEDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LTFNIVMRMVGGKKFC+E  KNNNVLE EGYCKRFKELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
           IDPSGY KRIMKIGR+ DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTD +YYS+ IIKG
Sbjct: 241 IDPSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           L+QDIL+AGIDTSAVTLQWALSHLLNNPIVL+KAKAEIDSYIGQERMVNE DLSSLSYLQ
Sbjct: 301 LVQDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKA 420
           GIISETLRLSPAGPLLVPHC+SEDCKIGGYDVPRNTIVLINAWAIHRD NLWEDASSFK 
Sbjct: 361 GIISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKP 420

Query: 421 RK 423
            +
Sbjct: 421 ER 420

[Close]

BLAST of Chy11G191610 vs. ExPASy TrEMBL
Match: A0A1S3C1S2 (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.2e-204
Identity = 359/423 (84.87%), Postives = 383/423 (90.54%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLN GKYL+YNNTTMVVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           G+HWRNLRRI TLEIFSTTRLNSFS+ REEEV+RLL KL GNYKLEDEFRVVELE MLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LTFNIVMRMVGGKKFC+E  KNNNVLE EGYCKRFKELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
           IDPSGY K IMKIGR+ DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTD +YYS+ IIKG
Sbjct: 241 IDPSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID  IG +R+V+EVDL SLSYLQ
Sbjct: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFK 420
           GIISETLRL P  PLLVPHCA  E+CKI  Y++PR+TIVLINAWAIHRD  LWEDA+ FK
Sbjct: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420

Query: 421 ARK 423
             +
Sbjct: 421 PER 421

[Close]

BLAST of Chy11G191610 vs. ExPASy TrEMBL
Match: A0A5D3BGH3 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00710 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.4e-181
Identity = 332/423 (78.49%), Postives = 356/423 (84.16%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLN GKYL+YNNTTMVVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           G+HWRNLRRI TLEIFSTTRLNSFS+ REEEV+RLL KL GNYKLEDEFRVVELE MLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LTFNIVMRMVGGKKFC+E  KNNNVLE EGYCKRFKEL                      
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKEL---------------------- 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
                    IMKIGR+ DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTD +YYS+ IIKG
Sbjct: 241 --------DIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID  IG +R+V+EVDL SLSYLQ
Sbjct: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFK 420
           GIISETLRL P  PLLVPHCA  E+CKI  Y++PR+TIVLINAWAIHRD  LWEDA+ FK
Sbjct: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 391

Query: 421 ARK 423
             +
Sbjct: 421 PER 391

[Close]

BLAST of Chy11G191610 vs. ExPASy TrEMBL
Match: A0A1S3C323 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.8e-169
Identity = 303/419 (72.32%), Postives = 346/419 (82.58%), Query Frame = 0


Query: 6   LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
           LL     L+S++  + FL +P  LNLPPTPLF LP IGHLHLLKHPVH+TLQ LSQKYGH
Sbjct: 5   LLSISFFLLSVVFAFKFLLRPRWLNLPPTPLFWLPLIGHLHLLKHPVHQTLQNLSQKYGH 64

Query: 66  VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPYGEHWR 125
           VFSL+FGSRLVV+VSSPSAVQECFTKNDIILANRP L  GKYL YN++++ +SPYGE WR
Sbjct: 65  VFSLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYGEQWR 124

Query: 126 NLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNY-KLE-DEFRVVELESMLLDLTF 185
           NLRRI +LE+FST RLN F  IRE+EV+RLL KLCGN+ KLE DEFRVVE+  +L DLT 
Sbjct: 125 NLRRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTL 184

Query: 186 NIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNWIDP 245
           NIVMRMV GK F            YE    +F+E+VTQIM HAG++NPGDFIPLWNWIDP
Sbjct: 185 NIVMRMVSGKMF------------YEEDSNKFREIVTQIMEHAGASNPGDFIPLWNWIDP 244

Query: 246 SGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKGLIQ 305
           +G+ K++ K+G+  DE LQ L+D IRN+ D GNTMI HLL LQ  + ++Y++   K +IQ
Sbjct: 245 TGFMKKVKKLGKTSDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQ 304

Query: 306 DILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGII 365
           DIL+AGIDTSAVTLQWALSHLLNNPIVL+KAKAEIDSYIGQERMVNE DLSSLSYLQGII
Sbjct: 305 DILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGII 364

Query: 366 SETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKARK 423
           SETLRLSPAGPLLVPHC+SEDCKIGGYDVPRNTIVLINAWAIHRD NLWEDASSFK  +
Sbjct: 365 SETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPER 411

[Close]

BLAST of Chy11G191610 vs. NCBI nr
Match: XP_004151924.2 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022055 [Cucumis sativus])

HSP 1 Score: 810 bits (2093), Expect = 7.36e-294
Identity = 402/422 (95.26%), Postives = 408/422 (96.68%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRLV+LVSSPSAVQECFTKNDIILANRPSLN GKYLSYNNTTM VSPY
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           GEHWRNLRRICTLEIFSTTRLNSFS+IREEEV+RLLCKLCGNYK EDEFRVVELESMLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LT NIVMRMVGGKKFCKENNKN NVLE EGYCKR KELVTQIMAHAGSTNPGDFIPLWNW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
           IDPSGYNKRIMKIGRRMDEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTD KYYS+LIIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKA 420
           GIISETLRLSP GPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRD N+WEDA SFK 
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420

Query: 421 RK 422
            +
Sbjct: 421 ER 422

[Close]

BLAST of Chy11G191610 vs. NCBI nr
Match: KAA0032538.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 774 bits (1999), Expect = 1.52e-279
Identity = 387/422 (91.71%), Postives = 400/422 (94.79%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLN GKYL+YNNTTMVVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           GEHWRNLRRI TLEIFSTTRLNSFS+ REEEV+RLL KL GNYKLEDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LTFNIVMRMVGGKKFC+E  KNNNVLE EGYCKRFKELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
           IDPSGY KRIMKIGR+ DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTD +YYS+ IIKG
Sbjct: 241 IDPSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           L+QDIL+AGIDTSAVTLQWALSHLLNNPIVL+KAKAEIDSYIGQERMVNE DLSSLSYLQ
Sbjct: 301 LVQDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKA 420
           GIISETLRLSPAGPLLVPHC+SEDCKIGGYDVPRNTIVLINAWAIHRD NLWEDASSFK 
Sbjct: 361 GIISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKP 420

Query: 421 RK 422
            +
Sbjct: 421 ER 420

[Close]

BLAST of Chy11G191610 vs. NCBI nr
Match: XP_008455822.2 (PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 716 bits (1848), Expect = 6.81e-257
Identity = 359/423 (84.87%), Postives = 383/423 (90.54%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLN GKYL+YNNTTMVVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           G+HWRNLRRI TLEIFSTTRLNSFS+ REEEV+RLL KL GNYKLEDEFRVVELE MLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LTFNIVMRMVGGKKFC+E  KNNNVLE EGYCKRFKELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
           IDPSGY K IMKIGR+ DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTD +YYS+ IIKG
Sbjct: 241 IDPSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID  IG +R+V+EVDL SLSYLQ
Sbjct: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCAS-EDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFK 420
           GIISETLRL P  PLLVPHCA  E+CKI  Y++PR+TIVLINAWAIHRD  LWEDA+ FK
Sbjct: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420

Query: 421 ARK 422
             +
Sbjct: 421 PER 421

[Close]

BLAST of Chy11G191610 vs. NCBI nr
Match: XP_038902403.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 705 bits (1820), Expect = 2.78e-252
Identity = 348/423 (82.27%), Postives = 380/423 (89.83%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFD L   LLSL+ LLLGY+ L KP+RLNLPPTPLF LPFIGHLHLLKHPVH+T Q LS
Sbjct: 1   MDFDILFCILLSLVCLLLGYTSLLKPNRLNLPPTPLFCLPFIGHLHLLKHPVHQTFQNLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGSRL+VLVSSPS VQECFTKNDIILANRPSLN GKYL+YNN TM VS Y
Sbjct: 61  QKYGHVFSLKFGSRLIVLVSSPSVVQECFTKNDIILANRPSLNTGKYLAYNNITMAVSSY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGN-YKLEDEFRVVELESMLL 180
           GEHWRNLRRI TLEIFS TR+NSF + RE+EV+RLLCKLCGN Y LED+FR V+L+ MLL
Sbjct: 121 GEHWRNLRRISTLEIFSMTRVNSFLRTREDEVKRLLCKLCGNCYTLEDKFRAVDLKPMLL 180

Query: 181 DLTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWN 240
           DLTFNIVMRMVGGKKFC+ENN NNNVLE EGY KRF+E+VTQIMAH+GSTNP DFIPLWN
Sbjct: 181 DLTFNIVMRMVGGKKFCEENN-NNNVLEDEGYSKRFREVVTQIMAHSGSTNPEDFIPLWN 240

Query: 241 WIDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIK 300
           WIDP+G+NKRIMK+GRR DE+LQGLVDEIRN+EDEGN MI HLLRLQ TD +YYS+ +IK
Sbjct: 241 WIDPTGFNKRIMKLGRRADEILQGLVDEIRNQEDEGNNMIHHLLRLQNTDLEYYSDQVIK 300

Query: 301 GLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYL 360
           GLI DILIAGIDT+AVTL+WALSHLLNNPIVL++AKAEID  IGQERMVNE DLSSLSYL
Sbjct: 301 GLIHDILIAGIDTTAVTLEWALSHLLNNPIVLERAKAEIDCSIGQERMVNEADLSSLSYL 360

Query: 361 QGIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFK 420
           QGIISETLRLSP GPLLVPHCASEDCKIGGYD+PR TIVLIN WAIHRD NLWEDA+SFK
Sbjct: 361 QGIISETLRLSPPGPLLVPHCASEDCKIGGYDIPRKTIVLINVWAIHRDPNLWEDATSFK 420

Query: 421 ARK 422
             +
Sbjct: 421 PER 422

[Close]

BLAST of Chy11G191610 vs. NCBI nr
Match: XP_038902241.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 698 bits (1802), Expect = 1.37e-249
Identity = 349/422 (82.70%), Postives = 375/422 (88.86%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
           MDFD LL  LLSL+ LLLGYSFL KPH LNLPPTPLF LPFIGHLHLLKHPVHK  Q LS
Sbjct: 1   MDFDLLLCILLSLVYLLLGYSFLLKPHGLNLPPTPLFCLPFIGHLHLLKHPVHKIFQNLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 120
           QKYGHVFSLKFGS LVVLVSSPSAVQECFTKNDIILANRPSLN GKYL+YNNT+M +SPY
Sbjct: 61  QKYGHVFSLKFGSHLVVLVSSPSAVQECFTKNDIILANRPSLNPGKYLAYNNTSMALSPY 120

Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 180
           GEHWRNLRRI TLEIFS TR N F K RE+EV+RLL KLCGN  LEDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRISTLEIFSATRFNLFLKSREDEVKRLLYKLCGNDTLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 240
           LTFNIVMRMVGGKKFC+ENNKN  V E EGY KRF+ELVTQIMAH GSTNPGDFIPLWNW
Sbjct: 181 LTFNIVMRMVGGKKFCEENNKN--VSEDEGYSKRFRELVTQIMAHGGSTNPGDFIPLWNW 240

Query: 241 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 300
           IDP+GYNKR+MK+GRR DEVLQGL+DEIRN+EDEG  MIQHLLRLQ TD +YYS+ +IKG
Sbjct: 241 IDPTGYNKRLMKLGRRSDEVLQGLIDEIRNQEDEGINMIQHLLRLQNTDPEYYSDQVIKG 300

Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
           L+QDIL+ GIDTSAVTL+WALSHLLNNPIVL++AKAEID +IGQERMVNE DLSSLSYLQ
Sbjct: 301 LVQDILLGGIDTSAVTLEWALSHLLNNPIVLERAKAEIDCFIGQERMVNEADLSSLSYLQ 360

Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKA 420
           GIISETLRLSP GPLL PH AS+DC IGGYDVPRNT+VL N WAIHRD NLWEDA+SFK 
Sbjct: 361 GIISETLRLSPPGPLL-PHYASKDCTIGGYDVPRNTMVLFNIWAIHRDPNLWEDATSFKP 419

Query: 421 RK 422
            +
Sbjct: 421 ER 419

[Close]

BLAST of Chy11G191610 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 428.7 bits (1101), Expect = 5.5e-120
Identity = 222/426 (52.11%), Postives = 298/426 (69.95%), Query Frame = 0


Query: 6   LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
           +LY++ SLI L++ + FL KP + NLPP+P   LP IGHL LLK P+H+TL++ S+   H
Sbjct: 9   VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68

Query: 66  -----VFSLKFGSRLVVLVSSPS-AVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSP 125
                V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT M+ +P
Sbjct: 69  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128

Query: 126 YGEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRL---LCKLCGNYKLEDEFRVVELES 185
           YG+HWRNLRR+CT+EIFST RLN F  +R +EVRRL   L +L G  K      VVEL+ 
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK-----TVVELKP 188

Query: 186 MLLDLTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIP 245
           ML+DLTFN +MRM+ GK++  E   +      E   KR ++LV  + A+  S N  D++P
Sbjct: 189 MLMDLTFNNIMRMMTGKRYYGEETTD------EEEAKRVRKLVADVGANTSSGNAVDYVP 248

Query: 246 LWNWIDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNL 305
           +      S Y  R+ K+G   D+ LQGL+D+ R +++ G TMI HLL LQK+D +YY++ 
Sbjct: 249 ILRLF--SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQ 308

Query: 306 IIKGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSL 365
           IIKG+I  ++IAG +TSAVTL+WALS+LLN+P V+ KA+ EID+ +G +R++ E DLS L
Sbjct: 309 IIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSEL 368

Query: 366 SYLQGIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDAS 423
            YL+ I+ ETLRL PA PLLVPH ASEDCKIG YD+PR T +L+NAWAIHRD N W+D  
Sbjct: 369 PYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPD 420

[Close]

BLAST of Chy11G191610 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 412.1 bits (1058), Expect = 5.3e-115
Identity = 213/424 (50.24%), Postives = 301/424 (70.99%), Query Frame = 0


Query: 6   LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
           +L+ +LSLI L+       KP   NLPP+P + LP IGHL LLK P+H+T  +LSQ   +
Sbjct: 10  ILFVVLSLIYLI--GKLKRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 69

Query: 66  --VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPYGEH 125
             +FSL+ G+RLV + SS S  +ECFTKND++LANRP+    K+++Y+ TTM+ + YG+H
Sbjct: 70  APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 129

Query: 126 WRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLDLTF 185
           WRNLRRI ++EIFS  RLNSF  IR++E+RRL+ +L  N+    EF  V+++SML DLTF
Sbjct: 130 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNF--SQEFVKVDMKSMLSDLTF 189

Query: 186 NIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNWIDP 245
           N ++RMV GK++  +       +E +   KR ++L+  ++A AG+ N  D++P+   +  
Sbjct: 190 NNILRMVAGKRYYGDG------VEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV-- 249

Query: 246 SGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKGLIQ 305
           S Y  R+ K+  R+DE LQGLVDE R  +++GNTMI HLL LQ++   Y+++ IIKG + 
Sbjct: 250 SDYETRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNML 309

Query: 306 DILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGII 365
            +++AG DTSAVTL+WALS++LN+P VL+KA+ EID  IG +R+++E D+S+L YLQ I+
Sbjct: 310 ALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIV 369

Query: 366 SETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKARKT 425
           SETLRL PA P+L+PH ASEDCK+ GYD+PR TI+L N WAIHRD  LW+D  SFK  + 
Sbjct: 370 SETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF 418

Query: 426 CKCG 428
            K G
Sbjct: 430 EKEG 418

[Close]

BLAST of Chy11G191610 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 406.0 bits (1042), Expect = 3.8e-113
Identity = 210/427 (49.18%), Postives = 290/427 (67.92%), Query Frame = 0


Query: 7   LYALLSL--ISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQK 66
           +Y +LSL    + L    LF  +P +LNLPP+P    P IGHLHLLK P+H+   +LS+ 
Sbjct: 11  IYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSES 70

Query: 67  YGH--VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMVVSPY 126
             +  +FSL  GSRLV +VSS +  +ECFTKND++LANRP    GK++ YN+TTMV + Y
Sbjct: 71  LNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAY 130

Query: 127 GEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELESMLLD 186
           G+ WRNLRRI T+EIFS+ RLNSF  IR++E+RRL+  L  N   +  F  VE++ + + 
Sbjct: 131 GDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKN--SQHGFVKVEMKPLFMC 190

Query: 187 LTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIPLWNW 246
           LT N ++RMV GK+F  +  +N+N        K  ++L+ +++   G+ N  D+ P+  +
Sbjct: 191 LTINNIIRMVAGKRFYGDGTENDN------EAKHVRQLIAEVVVSGGAGNAADYFPILRY 250

Query: 247 IDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNLIIKG 306
           +  + Y K + K+  R+DE LQ LV+E R E+ +GNTMI HLL LQ+T   YY+++IIKG
Sbjct: 251 V--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKG 310

Query: 307 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 366
           +I  +++AG DTSA TL+WA+S+LLN+P VL KAK EID  IG +R+V E D+  L YLQ
Sbjct: 311 IILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQ 370

Query: 367 GIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDASSFKA 426
            I+SETLRL P  P+L+PH ASEDC + GYDVPR TI+L+NAWAIHRD  LWE+   FK 
Sbjct: 371 HIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKP 427

Query: 427 RKTCKCG 428
            +  K G
Sbjct: 431 ERFEKKG 427

[Close]

BLAST of Chy11G191610 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 402.9 bits (1034), Expect = 3.2e-112
Identity = 210/431 (48.72%), Postives = 291/431 (67.52%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQT 60
           MD + +L  +LS +SL     FL      +LNLPP+P   LP IGHLHLLK P+H+T  +
Sbjct: 1   MDLNQIL--ILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLS 60

Query: 61  LSQKYGH--VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMV 120
           LS+  G+  VF L+ G+RLV ++SS S  +ECFTKND++LANRP     K+L YN T ++
Sbjct: 61  LSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLL 120

Query: 121 VSPYGEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELES 180
            + YG+HWRNLRRI  +EIFST RLNSF  IR++E+RRL+  L  +      F  VE+++
Sbjct: 121 SASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSL--HGFVEVEMKT 180

Query: 181 MLLDLTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIP 240
           +L +L  N  +RM+ GK++  E+N +          K  K LV++ +  AG+ NP D++ 
Sbjct: 181 LLTNLASNTTIRMLAGKRYFGEDNDD---------AKLVKNLVSEAVTSAGAGNPIDYLS 240

Query: 241 LWNWIDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNL 300
           +  W+  S Y KRI  +G R D  LQ LVDE R E+++G TMI HLL LQ     YY+++
Sbjct: 241 ILRWV--SSYEKRIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDV 300

Query: 301 IIKGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSL 360
           IIKG+I  ++IAG DTS+VTL+WA+S+LLN+P +L KA+ EID  +G +R+V+E D+ +L
Sbjct: 301 IIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNL 360

Query: 361 SYLQGIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDAS 420
           SYLQ I+ ETLR+ PA PLL+PH +SEDCK+GGYD+P  T+VL NAWA+HRD  +WED  
Sbjct: 361 SYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPE 416

Query: 421 SFKARKTCKCG 428
            FK  +  K G
Sbjct: 421 IFKPERFEKEG 416

[Close]

BLAST of Chy11G191610 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 402.5 bits (1033), Expect = 4.2e-112
Identity = 203/431 (47.10%), Postives = 297/431 (68.91%), Query Frame = 0


Query: 1   MDFDHLLYALLSLISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQT 60
           MD   +L  LLS + L +    L      + NLPP+P + LP IGHLHLLK PVH+T  +
Sbjct: 1   MDLTQIL--LLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHS 60

Query: 61  LSQKYGH--VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNFGKYLSYNNTTMV 120
           +S+  G+  +F L+ G+RLV ++SS S  +ECFTKND++LANRP +   K++ YN T M+
Sbjct: 61  ISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMI 120

Query: 121 VSPYGEHWRNLRRICTLEIFSTTRLNSFSKIREEEVRRLLCKLCGNYKLEDEFRVVELES 180
            + YG+HWRNLRRI  +EIFS+ R+++FS IR++E+RRL+  L  +      F  VEL+S
Sbjct: 121 AASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSL--HGFVEVELKS 180

Query: 181 MLLDLTFNIVMRMVGGKKFCKENNKNNNVLEYEGYCKRFKELVTQIMAHAGSTNPGDFIP 240
           +L +L FN ++ MV GK++     ++N+        K  +EL+ +IMA AGS N  D++P
Sbjct: 181 LLTNLAFNNIIMMVAGKRYYGTGTEDND------EAKLVRELIAEIMAGAGSGNLADYLP 240

Query: 241 LWNWIDPSGYNKRIMKIGRRMDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDSKYYSNL 300
             NW+  + +  +   +G R+D VLQ LVDE R E+++G T+I HLL  Q+T+ +YY+++
Sbjct: 241 SINWV--TNFENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDV 300

Query: 301 IIKGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSL 360
           IIKG+I  +++AG DTS+VTL+WA+S+LLN+P +L+KA+AEID  IG +R+V E D+ +L
Sbjct: 301 IIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNL 360

Query: 361 SYLQGIISETLRLSPAGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDLNLWEDAS 420
            YLQ I+SETLRL PA PLL+PH +S++CK+ GYD+PR T++L N WA+HRD  LWE+  
Sbjct: 361 HYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPE 419

Query: 421 SFKARKTCKCG 428
            FK  +  K G
Sbjct: 421 RFKPERFEKEG 419

The following BLAST results are available for this feature:

BLAST of Chy11G191610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FG65	7.7e-119	52.11	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
W8JMU7	6.1e-116	49.65	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ8	8.8e-115	50.59	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	6.3e-113	51.31	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q9LHA1	5.4e-112	49.18	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]

BLAST of Chy11G191610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQI5	3.1e-232	95.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1	[more]
A0A5A7SPU0	1.9e-221	91.71	Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A1S3C1S2	6.2e-204	84.87	LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L...	[more]
A0A5D3BGH3	7.4e-181	78.49	Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3C323	3.8e-169	72.32	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1	[more]

BLAST of Chy11G191610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151924.2	7.36e-294	95.26	cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022...	[more]
KAA0032538.1	1.52e-279	91.71	isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa]	[more]
XP_008455822.2	6.81e-257	84.87	PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo]	[more]
XP_038902403.1	2.78e-252	82.27	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_038902241.1	1.37e-249	82.70	cytochrome P450 81Q32-like [Benincasa hispida]	[more]

BLAST of Chy11G191610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36220.1	5.5e-120	52.11	cytochrome p450 81d1	[more]
AT4G37370.1	5.3e-115	50.24	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT3G28740.1	3.8e-113	49.18	Cytochrome P450 superfamily protein	[more]
AT4G37330.1	3.2e-112	48.72	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37320.1	4.2e-112	47.10	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 308..325 score: 39.44 coord: 361..372 score: 39.82
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 32..419 e-value: 2.6E-72 score: 244.0
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 401..425 score: 20.98 coord: 360..378 score: 35.73 coord: 317..343 score: 37.3 coord: 84..105 score: 20.89 coord: 60..79 score: 39.83 coord: 297..314 score: 29.02 coord: 181..199 score: 20.92
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 24..422 e-value: 2.8E-96 score: 324.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..419
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 7..420
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 7..420

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy11G191610.1	Chy11G191610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen