Chy11G189520 (gene) Cucumber (hystrix) v1

Overview


Name	Chy11G189520
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	AdoMet-dependent rRNA methyltransferase spb1
Location	chrH11: 3434718 .. 3435191 (+)
RNA-Seq Expression	Chy11G189520
Synteny	Chy11G189520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGTCAAGGGAAAGCATCGTTTGGACAAGTACTATCGCCTTGCCAAAGAGCATGGCTATCGCTCTCGTGCCTCGTGGAAACTCGCCCAGCTCGATTCCAAATACAACTTCCTCCGTTCTTCCCATGCCGTCCTCGATCTTTGCGCCGCCCCTGGTGGTTGGATGCAAGTCGCCGTCGAGCGGGTTCCTGTCGGTAGTCTCGTGGTTGGTGTTGATTTGGTTCCCATTGCTCCCGTCCGCGGTGCTGTCGCTTTCGAGCAAGATATCACCAAGCCGGAGTGTAAAGCCAGGCTCAAGAAGATTATGAGCGAAAAGGGGTGCGCTGCTTTTGATTTGATCTTGCACGATGGTTCGCCCAATGTTGGTGGTGCTTGGGCGCAGGAGGCTATGGCTCAGAATTCGTTGGTTATTGACTCTGTCAGATTAGCTACTCAGTTGTTGGCTCCGAAGGGTACATTTGTTACCAAG

mRNA sequence

ATGGGCAAAGTCAAGGGAAAGCATCGTTTGGACAAGTACTATCGCCTTGCCAAAGAGCATGGCTATCGCTCTCGTGCCTCGTGGAAACTCGCCCAGCTCGATTCCAAATACAACTTCCTCCGTTCTTCCCATGCCGTCCTCGATCTTTGCGCCGCCCCTGGTGGTTGGATGCAAGTCGCCGTCGAGCGGGTTCCTGTCGGTAGTCTCGTGGTTGGTGTTGATTTGGTTCCCATTGCTCCCGTCCGCGGTGCTGTCGCTTTCGAGCAAGATATCACCAAGCCGGAGTGTAAAGCCAGGCTCAAGAAGATTATGAGCGAAAAGGGGTGCGCTGCTTTTGATTTGATCTTGCACGATGGTTCGCCCAATGTTGGTGGTGCTTGGGCGCAGGAGGCTATGGCTCAGAATTCGTTGGTTATTGACTCTGTCAGATTAGCTACTCAGTTGTTGGCTCCGAAGGGTACATTTGTTACCAAG

Coding sequence (CDS)

ATGGGCAAAGTCAAGGGAAAGCATCGTTTGGACAAGTACTATCGCCTTGCCAAAGAGCATGGCTATCGCTCTCGTGCCTCGTGGAAACTCGCCCAGCTCGATTCCAAATACAACTTCCTCCGTTCTTCCCATGCCGTCCTCGATCTTTGCGCCGCCCCTGGTGGTTGGATGCAAGTCGCCGTCGAGCGGGTTCCTGTCGGTAGTCTCGTGGTTGGTGTTGATTTGGTTCCCATTGCTCCCGTCCGCGGTGCTGTCGCTTTCGAGCAAGATATCACCAAGCCGGAGTGTAAAGCCAGGCTCAAGAAGATTATGAGCGAAAAGGGGTGCGCTGCTTTTGATTTGATCTTGCACGATGGTTCGCCCAATGTTGGTGGTGCTTGGGCGCAGGAGGCTATGGCTCAGAATTCGTTGGTTATTGACTCTGTCAGATTAGCTACTCAGTTGTTGGCTCCGAAGGGTACATTTGTTACCAAG

Protein sequence

MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGSPNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK

Homology

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BLAST of Chy11G189520 vs. ExPASy Swiss-Prot
Match: Q5BH88 (AdoMet-dependent rRNA methyltransferase spb1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=spb1 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 8.2e-42
Identity = 85/156 (54.49%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+RA++KL QL+ KY FL  S  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYRLAKEKGYRARAAFKLVQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGSPN 122
            +P  SL+VGVDL PI P+   + F+QDIT  +C+A ++  +        D +LHDG+PN
Sbjct: 65  CMPAQSLIVGVDLAPIKPIPRVITFQQDITTEKCRATIRSHLKHWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           VG AW Q+A +Q  LV++S++LAT+ L   GTFVTK
Sbjct: 125 VGTAWVQDAFSQAELVLESLKLATEFLVEGGTFVTK 157

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BLAST of Chy11G189520 vs. ExPASy Swiss-Prot
Match: Q4WVH3 (AdoMet-dependent rRNA methyltransferase spb1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=spb1 PE=3 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-40
Identity = 82/156 (52.56%), Postives = 109/156 (69.87%), Query Frame = 0


Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+RA++KL QL+ KY FL  S  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYRLAKEKGYRARAAFKLIQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGSPN 122
            +P  S+++GVDL PI P+   + F+ DIT  +C+A +++ +        D +LHDG+PN
Sbjct: 65  CMPTQSIIIGVDLAPIKPIPRVITFQSDITTEKCRATIRQHLKHWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           VG AW Q+A +Q  LV+ S++LAT+ L   GTFVTK
Sbjct: 125 VGTAWVQDAFSQAELVLQSMKLATEFLVEGGTFVTK 157

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BLAST of Chy11G189520 vs. ExPASy Swiss-Prot
Match: Q4P6G5 (AdoMet-dependent rRNA methyltransferase SPB1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=SPB1 PE=3 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-40
Identity = 81/156 (51.92%), Postives = 111/156 (71.15%), Query Frame = 0


Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K   K RLDK+Y LAKE GYRSRA++KL QL+ K+NFL  +   +DLCAAPGGW+QVA +
Sbjct: 7   KKTAKGRLDKFYWLAKEQGYRSRAAFKLVQLNKKFNFLEKARCCIDLCAAPGGWLQVASK 66

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGSPN 122
            +P  SL+VGVDLVPI P+   + F +DI   +C+ +L++I+ +      D+++HDG+PN
Sbjct: 67  FMPANSLIVGVDLVPIKPIPRTITFAEDINSYKCRDQLRQILKDWKA---DIVIHDGAPN 126

Query: 123 VGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           VG AW Q+A AQ+ L + S+RLA + L   GTFVTK
Sbjct: 127 VGTAWVQDAYAQSELTLQSLRLAVEFLTAGGTFVTK 159

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BLAST of Chy11G189520 vs. ExPASy Swiss-Prot
Match: P0CS79 (AdoMet-dependent rRNA methyltransferase SPB1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=SPB1 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 4.5e-40
Identity = 82/156 (52.56%), Postives = 109/156 (69.87%), Query Frame = 0


Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+R+++KL  L+ KY+ L  +   +DLCAAPGGW+QVA +
Sbjct: 6   KKTGKGRLDKFYRLAKEQGYRARSAFKLVHLNRKYDLLSKARCCIDLCAAPGGWLQVAEK 65

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGSPN 122
            +P GSL++GVDL  I P+     F  DIT P C+  L++ M +      DL+LHDG+PN
Sbjct: 66  YMPKGSLIIGVDLNAIKPLPHVTTFVADITTPHCRQTLRQHMHDWKA---DLVLHDGAPN 125

Query: 123 VGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           VG AW Q+A  QN LV+ S++LAT+ LA  G+FVTK
Sbjct: 126 VGSAWVQDAFTQNELVLQSLKLATEFLAKGGSFVTK 158

[Close]

BLAST of Chy11G189520 vs. ExPASy Swiss-Prot
Match: P0CS78 (AdoMet-dependent rRNA methyltransferase SPB1 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=SPB1 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 4.5e-40
Identity = 82/156 (52.56%), Postives = 109/156 (69.87%), Query Frame = 0


Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+R+++KL  L+ KY+ L  +   +DLCAAPGGW+QVA +
Sbjct: 6   KKTGKGRLDKFYRLAKEQGYRARSAFKLVHLNRKYDLLSKARCCIDLCAAPGGWLQVAEK 65

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGSPN 122
            +P GSL++GVDL  I P+     F  DIT P C+  L++ M +      DL+LHDG+PN
Sbjct: 66  YMPKGSLIIGVDLNAIKPLPHVTTFVADITTPHCRQTLRQHMHDWKA---DLVLHDGAPN 125

Query: 123 VGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           VG AW Q+A  QN LV+ S++LAT+ LA  G+FVTK
Sbjct: 126 VGSAWVQDAFTQNELVLQSLKLATEFLAKGGSFVTK 158

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BLAST of Chy11G189520 vs. ExPASy TrEMBL
Match: A0A0A0LIR7 (Putative rRNA methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G248140 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 7.6e-83
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 158

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BLAST of Chy11G189520 vs. ExPASy TrEMBL
Match: A0A5A7TQD1 (Putative rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00370 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 8.4e-82
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVA EQDITKPECKARLKKIMSEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           PNVGGAWAQEAM+QNSLVIDSVRLATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVRLATQLLAPKGTFVTK 158

[Close]

BLAST of Chy11G189520 vs. ExPASy TrEMBL
Match: A0A1S3CD44 (Putative rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499682 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 8.4e-82
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVA EQDITKPECKARLKKIMSEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           PNVGGAWAQEAM+QNSLVIDSVRLATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVRLATQLLAPKGTFVTK 158

[Close]

BLAST of Chy11G189520 vs. ExPASy TrEMBL
Match: A0A1S4E314 (Putative rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499682 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 8.4e-82
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVA EQDITKPECKARLKKIMSEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           PNVGGAWAQEAM+QNSLVIDSVRLATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVRLATQLLAPKGTFVTK 158

[Close]

BLAST of Chy11G189520 vs. ExPASy TrEMBL
Match: A0A6J1FTB8 (Putative rRNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111448289 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.1e-80
Identity = 152/158 (96.20%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+A EQDITKPECKARLKKIM+EKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           PNVGGAWAQEAM+QNSLVIDSV+LATQLLAPKG FVTK
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTK 158

[Close]

BLAST of Chy11G189520 vs. NCBI nr
Match: XP_011649233.1 (adoMet-dependent rRNA methyltransferase spb1 [Cucumis sativus] >KGN61805.1 hypothetical protein Csa_006737 [Cucumis sativus])

HSP 1 Score: 315 bits (806), Expect = 1.53e-99
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 158
           PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 158

[Close]

BLAST of Chy11G189520 vs. NCBI nr
Match: XP_016902621.1 (PREDICTED: adoMet-dependent rRNA methyltransferase spb1 isoform X2 [Cucumis melo])

HSP 1 Score: 311 bits (797), Expect = 2.93e-98
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVA EQDITKPECKARLKKIMSEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 158
           PNVGGAWAQEAM+QNSLVIDSVRLATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVRLATQLLAPKGTFVTK 158

[Close]

BLAST of Chy11G189520 vs. NCBI nr
Match: XP_008460947.1 (PREDICTED: adoMet-dependent rRNA methyltransferase spb1 isoform X1 [Cucumis melo] >KAA0045562.1 adoMet-dependent rRNA methyltransferase spb1 isoform X1 [Cucumis melo var. makuwa] >TYK28831.1 adoMet-dependent rRNA methyltransferase spb1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 311 bits (797), Expect = 3.05e-98
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVA EQDITKPECKARLKKIMSEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 158
           PNVGGAWAQEAM+QNSLVIDSVRLATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVRLATQLLAPKGTFVTK 158

[Close]

BLAST of Chy11G189520 vs. NCBI nr
Match: XP_038901448.1 (adoMet-dependent rRNA methyltransferase spb1 [Benincasa hispida])

HSP 1 Score: 310 bits (793), Expect = 1.05e-97
Identity = 154/158 (97.47%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQ+A
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQIA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVA EQDITKPECKARLKKIMS+KGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSDKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 158
           PNVGGAWAQEAMAQNSLVIDSV+LATQLLAPKGTFVTK
Sbjct: 121 PNVGGAWAQEAMAQNSLVIDSVKLATQLLAPKGTFVTK 158

[Close]

BLAST of Chy11G189520 vs. NCBI nr
Match: XP_023512222.1 (adoMet-dependent rRNA methyltransferase spb1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 306 bits (785), Expect = 1.31e-96
Identity = 152/158 (96.20%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+A EQDITKPECKARLKKIM+EKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 158
           PNVGGAWAQEAM+QNSLVIDSV+LATQLLAPKG FVTK
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTK 158

[Close]

BLAST of Chy11G189520 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 249.2 bits (635), Expect = 2.2e-66
Identity = 119/158 (75.32%), Postives = 140/158 (88.61%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKE G+RSRAS+KL QLD+KY+ L S+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120
           VE+VPVGSLV+G+DLVPI PVRG V   QDIT+ ECK+++K++M + G +AF+L+LHDGS
Sbjct: 61  VEKVPVGSLVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQHGVSAFNLVLHDGS 120

Query: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
           PNVGGAWAQEAM+QN+LVIDSVRLAT+ LA  G  VTK
Sbjct: 121 PNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTK 158

[Close]

BLAST of Chy11G189520 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 9.6e-22
Identity = 61/172 (35.47%), Postives = 95/172 (55.23%), Query Frame = 0


Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGK   + + D YYR AKE G+R+R+++KL Q+D ++N       V+DLCAAPG W QV 
Sbjct: 1   MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVL 60

Query: 61  VER--VPVGS----------LVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSE-- 120
             +  +P  S          L+V +DL P+AP+ G +  + DIT     AR  +++    
Sbjct: 61  SRQLYLPAKSSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDIT----NARTAEVVIRHF 120

Query: 121 KGCAAFDLILHDGSPNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTK 159
            GC A DL++ DG+P+V G    +   Q+ L++  + + T +L   G F+ K
Sbjct: 121 DGCKA-DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAK 166

[Close]

BLAST of Chy11G189520 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.4e-09
Identity = 28/58 (48.28%), Postives = 39/58 (67.24%), Query Frame = 0


Query: 1  MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQ 59
          MGK   + + D YYR AKE G+R+R+++KL Q+D ++N       V+DLCAAPG W Q
Sbjct: 1  MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

[Close]

BLAST of Chy11G189520 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 57.8 bits (138), Expect = 9.3e-09
Identity = 42/151 (27.81%), Postives = 74/151 (49.01%), Query Frame = 0


Query: 11  DKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVERV-PV--G 70
           D +YR A+  GY +R+++KL Q+  +Y  ++   +VLDL  APG W+QVA + + P+  G
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSLGPLRSG 67

Query: 71  SLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSE-------------KGCAAFDL 130
            +VVG+D+  +               P+C +R++ I ++                  F +
Sbjct: 68  GIVVGMDIKKVK------------VPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSV 127

Query: 131 ILHDGSPNVGGAWAQEAMAQNSLVIDSVRLA 146
           IL D   +V G   ++A     L + ++ LA
Sbjct: 128 ILSDMCHSVSGITTRDAALSAELGMRALDLA 146

The following BLAST results are available for this feature:

BLAST of Chy11G189520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5BH88	8.2e-42	54.49	AdoMet-dependent rRNA methyltransferase spb1 OS=Emericella nidulans (strain FGSC...	[more]
Q4WVH3	1.2e-40	52.56	AdoMet-dependent rRNA methyltransferase spb1 OS=Neosartorya fumigata (strain ATC...	[more]
Q4P6G5	1.2e-40	51.92	AdoMet-dependent rRNA methyltransferase SPB1 OS=Ustilago maydis (strain 521 / FG...	[more]
P0CS79	4.5e-40	52.56	AdoMet-dependent rRNA methyltransferase SPB1 OS=Cryptococcus neoformans var. neo...	[more]
P0CS78	4.5e-40	52.56	AdoMet-dependent rRNA methyltransferase SPB1 OS=Cryptococcus neoformans var. neo...	[more]

BLAST of Chy11G189520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIR7	7.6e-83	100.00	Putative rRNA methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G248140 PE=3 ...	[more]
A0A5A7TQD1	8.4e-82	98.73	Putative rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3CD44	8.4e-82	98.73	Putative rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499682 PE=3 SV=...	[more]
A0A1S4E314	8.4e-82	98.73	Putative rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499682 PE=3 SV=...	[more]
A0A6J1FTB8	2.1e-80	96.20	Putative rRNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111448289 PE...	[more]

BLAST of Chy11G189520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011649233.1	1.53e-99	100.00	adoMet-dependent rRNA methyltransferase spb1 [Cucumis sativus] >KGN61805.1 hypot...	[more]
XP_016902621.1	2.93e-98	98.73	PREDICTED: adoMet-dependent rRNA methyltransferase spb1 isoform X2 [Cucumis melo...	[more]
XP_008460947.1	3.05e-98	98.73	PREDICTED: adoMet-dependent rRNA methyltransferase spb1 isoform X1 [Cucumis melo...	[more]
XP_038901448.1	1.05e-97	97.47	adoMet-dependent rRNA methyltransferase spb1 [Benincasa hispida]	[more]
XP_023512222.1	1.31e-96	96.20	adoMet-dependent rRNA methyltransferase spb1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy11G189520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25730.1	2.2e-66	75.32	FtsJ-like methyltransferase family protein	[more]
AT5G01230.1	9.6e-22	35.47	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G01230.2	1.4e-09	48.28	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G13830.1	9.3e-09	27.81	FtsJ-like methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015507	Ribosomal RNA large subunit methyltransferase E	PIRSF	PIRSF005461	23S_rRNA_mtase	coord: 2..158 e-value: 6.8E-43 score: 144.6
IPR002877	Ribosomal RNA methyltransferase FtsJ domain	PFAM	PF01728	FtsJ	coord: 22..158 e-value: 3.6E-36 score: 125.0
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 19..158 e-value: 3.0E-41 score: 143.2
None	No IPR available	PANTHER	PTHR10920:SF23	BNAA08G30800D PROTEIN	coord: 1..158
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 1..158
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 21..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy11G189520.1	Chy11G189520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000453	enzyme-directed rRNA 2'-O-methylation
biological_process	GO:0000466	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
biological_process	GO:0000463	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
biological_process	GO:0032259	methylation
biological_process	GO:0001510	RNA methylation
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0030687	preribosome, large subunit precursor
molecular_function	GO:0016435	rRNA (guanine) methyltransferase activity
molecular_function	GO:0008650	rRNA (uridine-2'-O-)-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity