Chy11G188650 (gene) Cucumber (hystrix) v1

Overview
NameChy11G188650
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAmidase domain-containing protein
LocationchrH11: 2608523 .. 2610929 (-)
RNA-Seq ExpressionChy11G188650
SyntenyChy11G188650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCTGCGCTCCCATGGCTGGCCAAAGCTTTCTCCCTTACATATCCATGCTTCTTGGTTTGATGGCGATTTTATCATCATGTGGGAGTTGTAGCTTTCACACTGAACTTTCCCTTGAAGAAGCAACTTTGAAAGATCTCCAACGTGCTTTCTACCAAAACAAACTCACCTCCAGGCGACTTGTCGAGTTTTATCTCGAACAAGTGCGTAGATTTAACCCCATCCTAAAAGGGATTATAGAAGTGAATCCAGATGCTTTAGACCAAGCCTCTCGAGCTGACCTCGAGCGCAAGAAAAGCTCACCAAGATCTTTGTCTCCACTTCATGGCATTCCTGTACTTGTAAAAGATAATATTGCAACCAAGGATAAGCTGAACACAACCGCTGGCTCTTTTGCTCTACTTGGCTCTGTTGTTCCTCGTGATGCAGGTGTGGTTACTAAGTTGAGGATGGCAGGTGCAATAATATTTGGAAAAGCGAGCCTGAGCGAGTGGTCTTACTTCAGGTCCAATTCACTTCCCAGCGGTTGGAGCGCTAGAGGTGGCCAAGGCAAGGTGTGCAACTTTTCCAACTTCAATAATTGTCTGTTTCTGAAAGAGCAAGATTATCACTTATTTGTTTGATAACACGGAGTTTCATCTCAAAATCAATTGATGATAATGAACGACGTAGTTTATCTATCTTAAAAAAAATTCGAGGTTCTTTCATTTTTCCAAGGTGGAATACACAATAAATCCATTAAGATGGAGCCACTTTTGAGTGTTGGAGATGATGAGAGTCCTATGTTAAAACAACTAAGGAGACTTACACTCCATATAATATAGATGAGCTACTCTTCTTACATTGAGTTTAACATTCTTGGACCGGATCCAAAGTTTTATTTTTAGACCTTGTCAAGACCTAAAACCTTACCCTTGATAAATTCAATTATACCAATGCTTATATATCTCTCGATTTTTAGCTGTTAGACATACTCAGATTCAGCAAATAGATAGTGATTTAAATTCAAAATATCTAATCAACATTAGGCTCTAAGTAAGTTAGATAGTCCAATTAAAAGAAAAGAGAATTTTGTTTTAAATCAACAACAAACTAAGATCACCCGTATGCGCCATATGTTTGCAGAATCCTTACACAATGGGAGAACCGTGTGGCTCGAGCAGCGGCTCTGCCATATCTGTTGCAGCAAATATGGTTACAGTTTCACTGGGGACCGAAACTGATGGATCAATATTATGCCCTTCTACTCTCAACTCAGTAGTTGGCATTAAACCCACAGTAGGTCTCACCAGTAGAGCAGGGGTCGTTCCGATCTCTTCGAGGCAGGACACTGTCGGGTAATCAATTTGTCATCATTGAAATTTTCCCATTTATATTTGTATCCAAAGGAAGTACTTTCTGCTGAATGAGTGATGATGAAACAGGCCTATTTGTAGAACAGTGTCAGATGCGGCTTATGTTCTAGAAGCCATTGTAGGAGCGGATAGATATGACAATTCAACAATTGAAGCATCAAAATACATTCCAGAAGGAGGATATGGCCAATTTCTAAGGGCTGGTGGGCTGAAAGGAAAGAGAATAGGAATTGTGAGGGAATTCTATGATTTTGGTCCTGATGACACTTTCTACACTCAAGCTTATGAGAAAGTCGTCAAAACCTTGAAGTAAAGCAATCTCACTTTTCATAAACCATCTTTTTTTATATATATAATTATGTTAGAGGTTTTTATCTGACCCTCTGTCATCAATAACATGAAAGGAAAGGAGGAGCAATATTGATGGACAATCTGACGATAGACAACCTAGAGCAAATCTTTGACGGTTCAAGTGGGGAACAGATCGCATTGCTTGCCGAATTCAAAATATCCTTAAATGCCTATCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAAAACTCAAAACTTGTGAGCGACTATCATCTATTCTGTTCTAATTTTACAAAATCCGCACAACACAAAAGGCAAACTATCATAACAATGAAATCTTATCACTATGTAGGAAAATCTAGAGTATGGTCAGGAGGAATTTCTAAAAGCAGAAGCCACCAATGGAATCGGAGATGTAGAAAAGGCAGCATTAGCCAGATTAGCAAAACTGTCAAAAGATGGATTTGAGAGACTGATGATTAAGAATAAGCTTGATTCAGTAGCAGCTCCTGGTTCGCTAATATCTCCTGTTTTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCAGCTGGATATGACCCTCAAGGGATTCCCTATGGTATCTGCTTTGGAGGATTAAAAGGATTCGAGCCAAGGCTGATAGAGATTGCATATGGATTTGAGCGTTTGACTAAGAGTAGAAAGCCCCCTTCAATTAAGAGACATTAA

mRNA sequence

ATGACCTGCGCTCCCATGGCTGGCCAAAGCTTTCTCCCTTACATATCCATGCTTCTTGGTTTGATGGCGATTTTATCATCATGTGGGAGTTGTAGCTTTCACACTGAACTTTCCCTTGAAGAAGCAACTTTGAAAGATCTCCAACGTGCTTTCTACCAAAACAAACTCACCTCCAGGCGACTTGTCGAGTTTTATCTCGAACAAGTGCGTAGATTTAACCCCATCCTAAAAGGGATTATAGAAGTGAATCCAGATGCTTTAGACCAAGCCTCTCGAGCTGACCTCGAGCGCAAGAAAAGCTCACCAAGATCTTTGTCTCCACTTCATGGCATTCCTGTACTTGTAAAAGATAATATTGCAACCAAGGATAAGCTGAACACAACCGCTGGCTCTTTTGCTCTACTTGGCTCTGTTGTTCCTCGTGATGCAGGTGTGGTTACTAAGTTGAGGATGGCAGGTGCAATAATATTTGGAAAAGCGAGCCTGAGCGAGTGGTCTTACTTCAGGTCCAATTCACTTCCCAGCGGTTGGAGCGCTAGAGGTGGCCAAGGCAAGAATCCTTACACAATGGGAGAACCGTGTGGCTCGAGCAGCGGCTCTGCCATATCTGTTGCAGCAAATATGGTTACAGTTTCACTGGGGACCGAAACTGATGGATCAATATTATGCCCTTCTACTCTCAACTCAGTAGTTGGCATTAAACCCACAGTAGGTCTCACCAGTAGAGCAGGGGTCGTTCCGATCTCTTCGAGGCAGGACACTGTCGGGCCTATTTGTAGAACAGTGTCAGATGCGGCTTATGTTCTAGAAGCCATTGTAGGAGCGGATAGATATGACAATTCAACAATTGAAGCATCAAAATACATTCCAGAAGGAGGATATGGCCAATTTCTAAGGGCTGGTGGGCTGAAAGGAAAGAGAATAGGAATTGTGAGGGAATTCTATGATTTTGGTCCTGATGACACTTTCTACACTCAAGCTTATGAGAAAGTCGTCAAAACCTTGAAGAAAGGAGGAGCAATATTGATGGACAATCTGACGATAGACAACCTAGAGCAAATCTTTGACGGTTCAAGTGGGGAACAGATCGCATTGCTTGCCGAATTCAAAATATCCTTAAATGCCTATCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAAAACTCAAAACTTGAAAATCTAGAGTATGGTCAGGAGGAATTTCTAAAAGCAGAAGCCACCAATGGAATCGGAGATGTAGAAAAGGCAGCATTAGCCAGATTAGCAAAACTGTCAAAAGATGGATTTGAGAGACTGATGATTAAGAATAAGCTTGATTCAGTAGCAGCTCCTGGTTCGCTAATATCTCCTGTTTTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCAGCTGGATATGACCCTCAAGGGATTCCCTATGGTATCTGCTTTGGAGGATTAAAAGGATTCGAGCCAAGGCTGATAGAGATTGCATATGGATTTGAGCGTTTGACTAAGAGTAGAAAGCCCCCTTCAATTAAGAGACATTAA

Coding sequence (CDS)

ATGACCTGCGCTCCCATGGCTGGCCAAAGCTTTCTCCCTTACATATCCATGCTTCTTGGTTTGATGGCGATTTTATCATCATGTGGGAGTTGTAGCTTTCACACTGAACTTTCCCTTGAAGAAGCAACTTTGAAAGATCTCCAACGTGCTTTCTACCAAAACAAACTCACCTCCAGGCGACTTGTCGAGTTTTATCTCGAACAAGTGCGTAGATTTAACCCCATCCTAAAAGGGATTATAGAAGTGAATCCAGATGCTTTAGACCAAGCCTCTCGAGCTGACCTCGAGCGCAAGAAAAGCTCACCAAGATCTTTGTCTCCACTTCATGGCATTCCTGTACTTGTAAAAGATAATATTGCAACCAAGGATAAGCTGAACACAACCGCTGGCTCTTTTGCTCTACTTGGCTCTGTTGTTCCTCGTGATGCAGGTGTGGTTACTAAGTTGAGGATGGCAGGTGCAATAATATTTGGAAAAGCGAGCCTGAGCGAGTGGTCTTACTTCAGGTCCAATTCACTTCCCAGCGGTTGGAGCGCTAGAGGTGGCCAAGGCAAGAATCCTTACACAATGGGAGAACCGTGTGGCTCGAGCAGCGGCTCTGCCATATCTGTTGCAGCAAATATGGTTACAGTTTCACTGGGGACCGAAACTGATGGATCAATATTATGCCCTTCTACTCTCAACTCAGTAGTTGGCATTAAACCCACAGTAGGTCTCACCAGTAGAGCAGGGGTCGTTCCGATCTCTTCGAGGCAGGACACTGTCGGGCCTATTTGTAGAACAGTGTCAGATGCGGCTTATGTTCTAGAAGCCATTGTAGGAGCGGATAGATATGACAATTCAACAATTGAAGCATCAAAATACATTCCAGAAGGAGGATATGGCCAATTTCTAAGGGCTGGTGGGCTGAAAGGAAAGAGAATAGGAATTGTGAGGGAATTCTATGATTTTGGTCCTGATGACACTTTCTACACTCAAGCTTATGAGAAAGTCGTCAAAACCTTGAAGAAAGGAGGAGCAATATTGATGGACAATCTGACGATAGACAACCTAGAGCAAATCTTTGACGGTTCAAGTGGGGAACAGATCGCATTGCTTGCCGAATTCAAAATATCCTTAAATGCCTATCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAAAACTCAAAACTTGAAAATCTAGAGTATGGTCAGGAGGAATTTCTAAAAGCAGAAGCCACCAATGGAATCGGAGATGTAGAAAAGGCAGCATTAGCCAGATTAGCAAAACTGTCAAAAGATGGATTTGAGAGACTGATGATTAAGAATAAGCTTGATTCAGTAGCAGCTCCTGGTTCGCTAATATCTCCTGTTTTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCAGCTGGATATGACCCTCAAGGGATTCCCTATGGTATCTGCTTTGGAGGATTAAAAGGATTCGAGCCAAGGCTGATAGAGATTGCATATGGATTTGAGCGTTTGACTAAGAGTAGAAAGCCCCCTTCAATTAAGAGACATTAA

Protein sequence

MTCAPMAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKGKRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDVEKAALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRH*
Homology
BLAST of Chy11G188650 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 5.4e-159
Identity = 302/510 (59.22%), Postives = 391/510 (76.67%), Query Frame = 0

Query: 11  FLPYISMLLGLMAILSSCGSCS---FHTELSLEEATLKDLQRAFYQNKLTSRRLVEFYLE 70
           F   + +L+   A++ S GS S     +  S++EAT++D++ AF + +LTS++LVE YLE
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 71  QVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNT 130
            + + NPIL  +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I+TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 131 TAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGKNP 190
           TAGSFALLGSVV RDAGVV +LR +GA+I GKASLSEW++FRS S+P GWSARG QGKNP
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 191 YTM-GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVV 250
           Y +   P GSSSGSAISV AN+V VSLGTETDGSIL P++ NSVVGIKP+VGLTSRAGVV
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 251 PISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKGK 310
           PIS RQD++GPICRTVSDA ++L+AIVG D  D +T  AS++IPEGGY QFL   GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 311 RIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGS-SGEQIAL 370
           R+GIV +          ++   +  +KTL++ GAI+++NLTI N+E I  G+ SGE+IAL
Sbjct: 309 RLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 371 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDVE 430
           LAEFK+SLNAYLKELV SP+RSL+D I +N++ ++ E + E+GQE FL AEAT+G+G+ E
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 431 KAALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYG 490
           K AL ++ +LS++G E+L+ +NKLD++   GS +S V AIGG+PG++VPAGYD  G+PYG
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 488

Query: 491 ICFGGLKGFEPRLIEIAYGFERLTKSRKPP 515
           I FGGL+  EP+LIEIA+ FE+ T  RKPP
Sbjct: 489 ISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of Chy11G188650 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.1e-79
Identity = 204/512 (39.84%), Postives = 287/512 (56.05%), Query Frame = 0

Query: 37  LSLEEATLKDLQRAFYQNKLTSRRLVEFYLEQVRRFNPILKGIIEVNPDALDQASRADLE 96
           ++LE+AT+  LQ       LTS  +V  YL++  + NP + GI+++NPD L  AS  D E
Sbjct: 64  ITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDE 123

Query: 97  RKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAII 156
           R     R   PLHGIP +VKDN ATKDK++TTAGS+ALLGS+VPRDA VV +LR AGA++
Sbjct: 124 RANGIIR--GPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVL 183

Query: 157 FGKASLSEWSYFRSNSLPSGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLGT 216
           FG A+LSEW+  RSN    G+SARGGQ + P+ +   P GSSSGSAISVA+NM+  +LGT
Sbjct: 184 FGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGT 243

Query: 217 ETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGA 276
           ETDGSI+ P+  N VVG+KPTVGLTSR GV+P S  QDT GPI RTV DA YV +++ G 
Sbjct: 244 ETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGI 303

Query: 277 DRYDNSTIEASKYIPE-GGYGQFL-RAGGLKGKRIGI--VREFYDFGPDDTFYTQAYEKV 336
           D  D  T+  +   PE G Y +FL     L+G R G+   R + +   D+        +V
Sbjct: 304 DENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADE---IDRLLEV 363

Query: 337 VKTLKKGGAILMDNLTIDNLEQIF-DG--------SSGEQIALLAEFKISLNAYLKELVA 396
           VK +++ GAI+ +N    NL+ I  DG        +  E   +  +F  ++ +YL E+  
Sbjct: 364 VKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKN 423

Query: 397 SPIRSLSDAIEFNKKNSKLEN--------LEYGQEEFLKAEATNGI-GDVEKAALARLAK 456
           + I SL D +E+N K    E            GQ+ FL +    G+  +    A+  + +
Sbjct: 424 TEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRR 483

Query: 457 LSKD-GFERLM---IKNKLDSVAAPGSLISPVFAI-------GGFPGVSVPAGYDPQGIP 515
            S+D G +  +        DS    G L+    +I        G+P +++P G    G P
Sbjct: 484 TSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRP 543

BLAST of Chy11G188650 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.0e-68
Identity = 180/495 (36.36%), Postives = 265/495 (53.54%), Query Frame = 0

Query: 47  LQRAFYQNKLTSRRLVEFYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLS 106
           LQ  + Q  +    +V+ Y+ ++   N  ++ + E+NPDAL  A + D ERK    R   
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLR--G 153

Query: 107 PLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWS 166
           PLHG+P+++K+NI T DK+++TAGS+A+ G+    DA V TKLR AG +I GK+  S+W+
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 167 YFRSNSLPSGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLGTETDGSILCPS 226
            FRS +  +GWSA GGQ    Y    +P GSSSGS ++    +   +LGTET GSI+ P+
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 227 TLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEA 286
             +++VG+KPTVGLTSR  VVPIS RQDTVGP+ R+V DAAY+L+ I G D  DN T  A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 287 SKYIPEGGYGQFLRAGGLKGKRIGIVREFYD-FGPDDTFYTQAYEKVVKTLKKGGAILMD 346
             +     Y +      LKGKRIG+ R     FG   T   Q + + +  +KK GAI+++
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQ-FNQALAVMKKAGAIIVE 393

Query: 347 NLTIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL 406
           N    +  +       + I L A+   +L A+ K+L  +P  +++D     +        
Sbjct: 394 NTDFTSFAEFAQSPIPDDI-LYADSLTNLPAFFKQLKVNP-HNITDLESLRRFTQHHRLE 453

Query: 407 EYGQEEFLKAEAT--NGIGDVEKAALARLAKLSKDGFERLMI----KNKLDSVAAPGSLI 466
           EY   +  + +     GI + +        K  K G E  ++    ++KLD+   P  L 
Sbjct: 454 EYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLS 513

Query: 467 SPVFAIGGFPGVSVPAGYDPQ---------------GIPYGICFGGLKGFEPRLIEIAYG 519
             + A+ G P ++VP G  P                GIP GI F G    E +LI +AY 
Sbjct: 514 PYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYA 573

BLAST of Chy11G188650 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.6e-41
Identity = 151/496 (30.44%), Postives = 240/496 (48.39%), Query Frame = 0

Query: 43  TLKDLQRAFYQNKLTSRRLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADLERKKSS 102
           +++ L +     + T+  +   +L +++   P +K  + + PD AL QA + D   K + 
Sbjct: 3   SIRQLHQQLVNKEKTAVEIATEFLARIQALEPQVKSFLHLTPDLALAQAQKVD--EKIAR 62

Query: 103 PRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKAS 162
             SL  L GIP+ +KDN+ TK  + TT  S  L   V P ++ V  KLR  GA+I GK +
Sbjct: 63  GESLHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGKTN 122

Query: 163 LSEWSYFRSNSLPSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGS 222
           L E++   S++  SG+        NP+ +   P GSS GSA +VAA    V+LG++T GS
Sbjct: 123 LDEFA-MGSSTENSGYHVTA----NPWDLSRVPGGSSGGSAAAVAAQECVVALGSDTGGS 182

Query: 223 ILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDN 282
           I  P++   VVG+KPT GL SR G+V  +S  D +GP  RTV DAA +L+AI G D  D+
Sbjct: 183 IRQPASFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDPQDS 242

Query: 283 STIEASKYIPEGGYGQFLRAGGLKGKRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGA 342
           +++     +P   Y QFL+   LKG +IG+++E +  G D     +A  + +  LK  GA
Sbjct: 243 TSLN----LPIPDYSQFLKT-SLKGLKIGVIKETFGEGLDQV-VAEAVNQALAQLKALGA 302

Query: 343 ILMDNLTIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAI--EFNKKN 402
                 TI  +             ++A  + S N    + V   IR  +D++   + K  
Sbjct: 303 ------TIKEISCPRFRYGLPTYYIIAPSEASANLARYDGVKYGIREDADSLIDMYTKTR 362

Query: 403 SKLENLEYGQEEFLKAEATN-GIGDVEKAALARLAKLSKDGFERLMIKNKLDSVAAPGS- 462
           +K    E  +   L     + G  D       ++  L K+ F+R      +D + +P S 
Sbjct: 363 AKGFGAEVKRRIMLGTYTLSAGYYDAYYLKAQKVRTLIKEDFDRAF--QSVDVLVSPTSP 422

Query: 463 --------------------LISPVFAIGGFPGVSVPAGYDPQGIPYGICFGGLKGFEPR 513
                               L++    + G PG+S+P G+D QG+P G+   G    E +
Sbjct: 423 TTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQLVGNVLREDQ 476

BLAST of Chy11G188650 vs. ExPASy Swiss-Prot
Match: Q8RC40 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=gatA PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 3.5e-41
Identity = 146/506 (28.85%), Postives = 252/506 (49.80%), Query Frame = 0

Query: 37  LSLEEATLKDLQRAFYQNKLTSRRLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADL 96
           + L   T+ +L+   ++ +++++ + + YLE+++   P +  ++ +  + AL++A  AD 
Sbjct: 1   MELYSLTVHELKELLHKREVSAQEVTKSYLERIKEVEPKIDALVTITEEFALERAKVADE 60

Query: 97  ERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAI 156
             K    + L+   GIPV++KDNI+T + + TT  S  L   + P +A VV +L   G +
Sbjct: 61  MIKNGEAKDLT---GIPVIIKDNIST-EGIRTTCSSKMLENYIPPYNATVVERLLNEGVV 120

Query: 157 IFGKASLSEWSYFRSNSLPSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLG 216
           I GK++L E++   S       ++     KNP+ +   P GSS GSA +VAA+    +LG
Sbjct: 121 ILGKSNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAVAADEAAFALG 180

Query: 217 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVG 276
           ++T GSI  P++L  VVG+KPT GL SR G+V  +S  D +GP  + V+D A VL AI G
Sbjct: 181 SDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNAIAG 240

Query: 277 ADRYDNSTIEASKYIPEGGYGQFLRAGGLKGKRIGIVREFYDFGPDDTFYTQAYEKVVKT 336
            D  D+++++  K  P+  Y  +L+   +KG RIG+ +EF+  G ++    +  EK +K 
Sbjct: 241 HDPMDSTSVKIEK--PD--YTSYLKE-DIKGLRIGVAKEFFGAGIEEG-VKETVEKAIKV 300

Query: 337 LKKGGAILMDNLTIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIE- 396
            +  GA ++D +++  +E          I   AE   +L  Y         ++  D ++ 
Sbjct: 301 FEDLGAEIID-ISVPYVEYAL---PAYYIIASAEASSNLARYDGIRYGHIAKNYEDLVDM 360

Query: 397 FNKKNSKLENLEYGQEEFLKAEA-TNGIGDVEKAALARLAKLSKDGFERLMIKNKLDSVA 456
           +    S+    E  +   L   A ++G  D       ++  L K+ FER     K D + 
Sbjct: 361 YMTSRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFERAF--EKCDVIV 420

Query: 457 APGS-----------------LISPVFA----IGGFPGVSVPAGYDPQGIPYGICFGGLK 516
            P S                  ++ ++     I G P +S+P G   +G+P G+   G  
Sbjct: 421 GPTSPTVAFKIGERTNDPLAMYLADIYTVSVNIAGLPAISIPCGLS-EGLPVGLQIIGKH 480

BLAST of Chy11G188650 vs. ExPASy TrEMBL
Match: A0A0A0LM97 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=4 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.4e-279
Identity = 502/513 (97.86%), Postives = 507/513 (98.83%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MAGQSFL  ISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSR+LVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           LEQVRR NPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN+LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIP+GGYGQFLRAGGLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
           KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAIL+DNL IDNLEQIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDVEK 425
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGD EK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 426 AALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGI 485
           AALARLAKLSKDGFERLMIKNKLD+VAAPGSLISPVFAIGGFPGVSVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

Query: 486 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR 519
           CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR
Sbjct: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR 513

BLAST of Chy11G188650 vs. ExPASy TrEMBL
Match: A0A5D3BKT1 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001390 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.1e-267
Identity = 477/511 (93.35%), Postives = 495/511 (96.87%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MAGQSFL Y+SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSR+LVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIP+GGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAIL+DN TIDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDVEK 425
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQE FLKAEATNGIGD EK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 426 AALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGI 485
           AALARLAKLSKDGFERLMIKNKLD++AAPGSLIS VFAIGGFPGVSVPAGYDPQG+PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 486 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 517
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of Chy11G188650 vs. ExPASy TrEMBL
Match: E5GC09 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.1e-267
Identity = 477/511 (93.35%), Postives = 495/511 (96.87%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MAGQSFL Y+SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSR+LVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIP+GGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAIL+DN TIDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDVEK 425
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQE FLKAEATNGIGD EK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 426 AALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGI 485
           AALARLAKLSKDGFERLMIKNKLD++AAPGSLIS VFAIGGFPGVSVPAGYDPQG+PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 486 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 517
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of Chy11G188650 vs. ExPASy TrEMBL
Match: A0A1S3C5K8 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.1e-267
Identity = 477/511 (93.35%), Postives = 495/511 (96.87%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MAGQSFL Y+SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSR+LVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIP+GGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAIL+DN TIDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDVEK 425
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQE FLKAEATNGIGD EK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 426 AALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGI 485
           AALARLAKLSKDGFERLMIKNKLD++AAPGSLIS VFAIGGFPGVSVPAGYDPQG+PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 486 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 517
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of Chy11G188650 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.1e-237
Identity = 438/517 (84.72%), Postives = 465/517 (89.94%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILS--SCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVE 65
           MA QSF  YISMLLGL+AILS    GSCSF T  S+EEATLKD Q AFYQNKLTSR+LVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 66  FYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKD 125
           FYLEQVRR NPILKGIIEVNPDAL+QAS+ADL+RK+SS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 126 KLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQ 185
           KLNTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRS   P+GWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 186 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 245
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 246 GVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGL 305
           GVVPIS RQDTVGPICRTV+DAAYVL+AI GADRYDNSTIEASKYIP GGYGQFLRA GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 306 KGKRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQI 365
           KGKRIGIVR+ YDFG DD FY  A+EKV KTLK+GGAIL+DNLTI+  + I   SSGE  
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 366 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGD 425
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLE L EYGQE FL+AEAT GIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 426 VEKAALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIP 485
            EKAALARLAKLSK+GFERLMIKNKLD++AAPG LISP  AIGGFPGVSVPAGY+PQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 486 YGICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRH 520
           +GI FGGLKGF+PRLIEIAYGFE LT  RK PS+ RH
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGRH 517

BLAST of Chy11G188650 vs. NCBI nr
Match: XP_004147023.1 (probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Csa_006678 [Cucumis sativus])

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 502/513 (97.86%), Postives = 507/513 (98.83%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MAGQSFL  ISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSR+LVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           LEQVRR NPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN+LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIP+GGYGQFLRAGGLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
           KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAIL+DNL IDNLEQIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDVEK 425
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGD EK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 426 AALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGI 485
           AALARLAKLSKDGFERLMIKNKLD+VAAPGSLISPVFAIGGFPGVSVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

Query: 486 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR 518
           CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR
Sbjct: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR 513

BLAST of Chy11G188650 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 929 bits (2401), Expect = 0.0
Identity = 477/511 (93.35%), Postives = 495/511 (96.87%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MAGQSFL Y+SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSR+LVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIP+GGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAIL+DN TIDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDVEK 425
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQE FLKAEATNGIGD EK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 426 AALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGI 485
           AALARLAKLSKDGFERLMIKNKLD++AAPGSLIS VFAIGGFPGVSVPAGYDPQG+PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 486 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 516
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of Chy11G188650 vs. NCBI nr
Match: XP_038900913.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 830 bits (2145), Expect = 3.29e-300
Identity = 432/515 (83.88%), Postives = 469/515 (91.07%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MA QSF  YISM+L L+AILSS G+CSF T   +EEAT+KDLQ AFYQNKLTSR+LV+FY
Sbjct: 1   MAAQSFWLYISMVLSLVAILSSYGNCSFGTHFPIEEATVKDLQLAFYQNKLTSRQLVKFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           L+QVRRFNPILKGIIEVNPDALDQA+RAD ERK+SS  SLSPLHGIPVL+KDNIATKDKL
Sbjct: 61  LKQVRRFNPILKGIIEVNPDALDQATRADHERKRSSLSSLSPLHGIPVLLKDNIATKDKL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRSN LP+GW ARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSQFRSN-LPNGWCARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPYTMGEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTYNSVVGIRPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           VPISSRQD VGPICRTVSDAAYVL+AIVGAD+YDNSTIEASKYIP+GGYGQFLR  GLKG
Sbjct: 241 VPISSRQDAVGPICRTVSDAAYVLDAIVGADKYDNSTIEASKYIPKGGYGQFLRPDGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
            RIGIVR+F+DFG D  FY++AYEKV K+LK+GGAIL+DNLTID+L+ I  G SGE  AL
Sbjct: 301 TRIGIVRKFFDFGDDGVFYSEAYEKVFKSLKQGGAILVDNLTIDSLDIIIGGLSGEWTAL 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDVE 425
            AEFKIS+NAYLKELVASPIRSLSDAIEFNKKNSKLE L EYGQ  FL+AEATNGIGDVE
Sbjct: 361 PAEFKISINAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQVLFLEAEATNGIGDVE 420

Query: 426 KAALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYG 485
           KAALA +A LSK+GFER+MIKNKLD++AAPG LISPV AIGGFPG+SVPAGY P G+PYG
Sbjct: 421 KAALASVASLSKNGFERVMIKNKLDAIAAPGGLISPVLAIGGFPGISVPAGYSPDGVPYG 480

Query: 486 ICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRH 519
           I FGGLKGFEPRLIEIAYGFE+LTKSRKPP IKRH
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEQLTKSRKPPLIKRH 514

BLAST of Chy11G188650 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 830 bits (2144), Expect = 5.22e-300
Identity = 438/517 (84.72%), Postives = 465/517 (89.94%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILS--SCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVE 65
           MA QSF  YISMLLGL+AILS    GSCSF T  S+EEATLKD Q AFYQNKLTSR+LVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 66  FYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKD 125
           FYLEQVRR NPILKGIIEVNPDAL+QAS+ADL+RK+SS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 126 KLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQ 185
           KLNTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRS   P+GWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 186 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 245
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 246 GVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGL 305
           GVVPIS RQDTVGPICRTV+DAAYVL+AI GADRYDNSTIEASKYIP GGYGQFLRA GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 306 KGKRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQI 365
           KGKRIGIVR+ YDFG DD FY  A+EKV KTLK+GGAIL+DNLTI+  + I   SSGE  
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 366 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGD 425
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLE L EYGQE FL+AEAT GIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 426 VEKAALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIP 485
            EKAALARLAKLSK+GFERLMIKNKLD++AAPG LISP  AIGGFPGVSVPAGY+PQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 486 YGICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRH 519
           +GI FGGLKGF+PRLIEIAYGFE LT  RK PS+ RH
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGRH 517

BLAST of Chy11G188650 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 829 bits (2141), Expect = 1.39e-299
Identity = 432/515 (83.88%), Postives = 464/515 (90.10%), Query Frame = 0

Query: 6   MAGQSFLPYISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRRLVEFY 65
           MA QSF  YISMLLGL+AILSS GSCSF T  S+EEATLKDLQ AFYQNKLTS +LVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 66  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 125
           LEQVRRFNPIL GIIEVNPDAL+QAS+ADLERK+SSPRSLSPLHGIPVLVKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 126 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRS   P+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 186 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 245
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKG 305
           VPIS RQDTVGPICRTV+DAAYVL+AI G DRYDNSTIEASKY+P+GGYGQFL+  GLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 306 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGSSGEQIAL 365
           KRIGIVR+ YDFG DD FY  A+EKV KTLK+GGAIL+DNLTI++   I   SSGE  A+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 366 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDVE 425
           LAEFKIS+N YLK+LVASPIRSLSDAIEFN+KNSKLE L EYGQE FL+AEAT GIG  E
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 426 KAALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYG 485
           KAALARLAKLSK+GFERLMIKNKLD++AAPG LIS   AIGGFPGVSVPAGY+PQG+P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 486 ICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRH 519
           I FGGLKGFEPRLIEIAYGFE LT  RK PS+ RH
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGRH 515

BLAST of Chy11G188650 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 3.0e-136
Identity = 269/509 (52.85%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 11  FLPYISMLLGLMAILSSCGSCS---FHTELSLEEATLKDLQRAFYQNKLTSRRLVEFYLE 70
           F   + +L+   A++ S GS S     +  S++EAT++D++ AF + +LTS++LVE YLE
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 71  QVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNT 130
            + + NPIL  +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I+TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 131 TAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSARGGQGKNP 190
           TAGSFALLGSVV RDAGVV +LR +GA+I GKASLSEW++FRS S+P GWSA        
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------- 188

Query: 191 YTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVP 250
                                               PS  NSVVGIKP+VGLTSRAGVVP
Sbjct: 189 ------------------------------------PSQ-NSVVGIKPSVGLTSRAGVVP 248

Query: 251 ISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFLRAGGLKGKR 310
           IS RQD++GPICRTVSDA ++L+AIVG D  D +T  AS++IPEGGY QFL   GLKGKR
Sbjct: 249 ISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKR 308

Query: 311 IGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQIFDGS-SGEQIALL 370
           +GIV +          ++   +  +KTL++ GAI+++NLTI N+E I  G+ SGE+IALL
Sbjct: 309 LGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALL 368

Query: 371 AEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDVEK 430
           AEFK+SLNAYLKELV SP+RSL+D I +N++ ++ E + E+GQE FL AEAT+G+G+ EK
Sbjct: 369 AEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEK 428

Query: 431 AALARLAKLSKDGFERLMIKNKLDSVAAPGSLISPVFAIGGFPGVSVPAGYDPQGIPYGI 490
            AL ++ +LS++G E+L+ +NKLD++   GS +S V AIGG+PG++VPAGYD  G+PYGI
Sbjct: 429 TALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGI 462

Query: 491 CFGGLKGFEPRLIEIAYGFERLTKSRKPP 515
            FGGL+  EP+LIEIA+ FE+ T  RKPP
Sbjct: 489 SFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of Chy11G188650 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 133.7 bits (335), Expect = 4.4e-31
Identity = 146/542 (26.94%), Postives = 246/542 (45.39%), Query Frame = 0

Query: 2   TCAPMAGQSFLPYISMLLGLMAILSSCGSCSFHTE-LSLEEATLKDLQRAFYQNKLTSRR 61
           T  P    S LP     +    I+S+  S +  T  +S  ++ +   +R+    + T+  
Sbjct: 4   TLQPPRSLSLLPLRRFQIS-KTIVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVE 63

Query: 62  LVEFYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIA 121
           + + YL ++R   P LK  + V+ + L  A   +++++ +    L PL G+ + VKDNI 
Sbjct: 64  IAKSYLSRIRLTEPQLKCFLHVSENVLKDAQ--EIDQRIAKGEELGPLAGVLIGVKDNIC 123

Query: 122 TKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSLPSGWSAR 181
           T+  + +TA S  L     P DA  V K++  G I+ GK ++ E+    S +  S +   
Sbjct: 124 TQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFG-MGSTTEASAFQVT 183

Query: 182 GGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 241
                NP+ +   P GSS GSA +VAA    VSLG++T GS+  P++   VVG+KPT G 
Sbjct: 184 A----NPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGR 243

Query: 242 TSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPEGGYGQFL- 301
            SR G++  +S  D +G    TV+DA  +L AI G DR+D+++  + + +PE    QFL 
Sbjct: 244 VSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTS--SKQDVPE-FQSQFLS 303

Query: 302 ----RAGGLKGKRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILMDNLTIDNLEQI 361
                +  L G ++GI+RE  + G  D+    A ++    L+  G I    LT  +L   
Sbjct: 304 VDHFESKPLNGVKVGIIRETLEDGV-DSGVRSATQEAASHLEALGCI----LTEVSLPSF 363

Query: 362 FDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKA 421
             G     +   +E   +L+ Y      + +     A E NK         +G E  ++ 
Sbjct: 364 SLGLPAYYVIASSESSSNLSRYDGVRYGNQVM----AEELNKLYECSRGEGFGGEVKMRI 423

Query: 422 -----EATNGIGDVEKAALARLAKLSKDGFERLMIKNK-LDSVAAP-------------- 481
                  + G  D       ++  L +  F+  + +N  L S AAP              
Sbjct: 424 LMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPL 483

Query: 482 ----GSLISPVFAIGGFPGVSVPAGY---DPQGIPYGICFGGLKGFEPRLIEIAYGFERL 510
               G +++    + G P + +P G     P G+P G+   G    E +L+++ + FE+ 
Sbjct: 484 AMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQT 524

BLAST of Chy11G188650 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 119.8 bits (299), Expect = 6.6e-27
Identity = 82/232 (35.34%), Postives = 125/232 (53.88%), Query Frame = 0

Query: 55  KLTSRRLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADLERKKSSPRSLSPLHGIPV 114
           ++TS+ LV  YL+Q++R+N +L+ ++    + A  QA  AD     S    L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 115 LVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSL 174
            +KD +A      TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 175 PSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 234
              W   GG+ +NP+ + E   GSS+G A S +A MV  ++G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITA 395

Query: 235 IKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIE 285
           ++PT G   R GV+ IS   D +GP CRT +D A +L+AI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 436

BLAST of Chy11G188650 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 109.4 bits (272), Expect = 8.9e-24
Identity = 80/232 (34.48%), Postives = 121/232 (52.16%), Query Frame = 0

Query: 55  KLTSRRLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADLERKKSSPRSLSPLHGIPV 114
           ++TS+ LV  YL+Q++R+N +L+ ++    + A  QA  AD     S    L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 115 LVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNSL 174
            +KD +A      TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 175 PSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 234
              W   GG+ +NP+ + E   GSS+G A S +A       G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITA 395

Query: 235 IKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIE 285
           ++PT G   R GV+ IS   D +GP CRT +D A +L+AI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 429

BLAST of Chy11G188650 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 103.6 bits (257), Expect = 4.9e-22
Identity = 115/487 (23.61%), Postives = 206/487 (42.30%), Query Frame = 0

Query: 52  YQNKLT-----SRRLVEFYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLS 111
           Y++KLT     ++R++   +E+     P    +I  + + + + + A   R +     +S
Sbjct: 138 YRSKLTTPLQVAKRIISI-IEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQG-NPIS 197

Query: 112 PLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWS 171
            L GI V +KD+I           ++      V +D+ VV+KLR  GAI+ GKA++ E  
Sbjct: 198 VLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 257

Query: 172 YFRSNSLPSGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPST 231
              + +  +  + R       YT     GSSSGSA  VAA + + +LGT+  GS+  PS 
Sbjct: 258 MGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSA 317

Query: 232 LNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEAS 291
           L  + G+K T G T   G +      + +GP+  ++ DA  V  AI+G+   D   ++ S
Sbjct: 318 LCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSADRYNLKPS 377

Query: 292 -----KYIPEGGYGQFLRAGGLKGKRIG-IVREFYDFGPDDTFYTQAYEKVVKTLKKGGA 351
                K +   G      +  +   R+G   + F D    D   +   E ++K L     
Sbjct: 378 PPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSD--ISDKCEDILKLLSNNHG 437

Query: 352 ILMDNLTIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSK 411
             +  + +  LE++    +   I++ +    SL  Y +          +  + ++ + S 
Sbjct: 438 CKVVEIVVPELEEM---RAAHVISIGSPTLSSLTPYCE-------AGKNSKLSYDTRTSF 497

Query: 412 LENLEYGQEEFLKAEA---------TNGIGDVEKAALARLAKLSKDGFERLMIKNKLDSV 471
                +   +++ A+           N   DV+   +     ++        +KN   ++
Sbjct: 498 AIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVD-VIVTPTTGMTAPVIPPDALKNGETNI 557

Query: 472 AAPGSLISPVFAIG--GFPGVSVPAGYDPQGIPYGICFGGLKGFEPRLIEIAYGFERLTK 517
                L+  V A    GFP +SVP GYD +G+P G+   G    E  ++ +A   E L  
Sbjct: 558 QVTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAAVEELAP 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7605.4e-15959.22Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY41.1e-7939.84Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C82.0e-6836.36Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0JSX31.6e-4130.44Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain ... [more]
Q8RC403.5e-4128.85Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus s... [more]
Match NameE-valueIdentityDescription
A0A0A0LM972.4e-27997.86Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=... [more]
A0A5D3BKT18.1e-26793.35Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001... [more]
E5GC098.1e-26793.35Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3C5K88.1e-26793.35putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1[more]
A0A5D3BN385.1e-23784.72Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
XP_004147023.10.097.86probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Cs... [more]
XP_008457661.10.093.35PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis ... [more]
XP_038900913.13.29e-30083.88probable amidase At4g34880 [Benincasa hispida][more]
XP_008457659.15.22e-30084.72PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 puta... [more]
XP_004147024.31.39e-29983.88probable amidase At4g34880 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G34880.13.0e-13652.85Amidase family protein [more]
AT3G25660.14.4e-3126.94Amidase family protein [more]
AT5G07360.16.6e-2735.34Amidase family protein [more]
AT5G07360.28.9e-2434.48Amidase family protein [more]
AT5G64440.14.9e-2223.61fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 30..516
e-value: 8.4E-143
score: 479.0
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 38..514
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 60..458
e-value: 4.4E-72
score: 243.5
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 23..517
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 23..517

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G188650.1Chy11G188650.1mRNA