Chy11G188480 (gene) Cucumber (hystrix) v1

Overview
NameChy11G188480
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionRhodanese-like domain-containing protein 19
LocationchrH11: 2493014 .. 2494137 (+)
RNA-Seq ExpressionChy11G188480
SyntenyChy11G188480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGGTTAATCTTATCAACCATGTTGCCATTTCGTTATCTCGTAGGATGTAACCTCACATTTACTATGCAAAAAAAGAGTTTGAAATTAACTCATTTATTCATTCCCAATCTGCAGGACAGTCGATGTTCAAGTTGCCAAAGATCTCTTAGAAAAGGGTCGCCTCTGTTTGGATGTCAGGTCGGCTTATATATAAATATATATAATCAAAGGTAATAAAGCTAAATAACTGAAGAGATGGTTTTGGTTGCAGAACAGTTGAAGAGTATAACAAAGGCCATGTTGAGAATGCACTAAATGTTCCTTACGTGTTCTTCACCCCAGAAGGTAATAACCAGCTGTCCACTACCTGTATGATTTTGTTCTAAAAGTAAGCTAAACTCTACAGTTTTAGGATGTTTTCACTGCTAAGCATTGACCTAGATGCATTTTCTTAGATCAACTCTCGTATGTTTGATCCTATAGAATCTGACAGTTTGCTAACAAACAGGTCAAGTAAAGAATCCCGACTTTCTAGCACAAGTTACATCCATATTAAAGAAGGAAGATCATATAGTAGTGGTAAGAAGAAAAACTATTCCTCGGTGTTGCTACTTTTATATCCCAATGCAGATTCAAGAGTAAAATTGAAACAAATTGCAAATTATTTACCTATTTGTGCAGAATTGCAATAGAGGTGGTAGAGGCCTTCGTGCTTGCGTCGATCTTCTTAACGCGGTAAGATTTGAGATGCTAGAACTAGTTTGCAAGGTTGACTAGACCTGCAAAAGAAAAGGATAAGTAATAATAAGATTTAGTGAGAAATTTCAGGGCTTCGAGCATGTGAATAACATGGGAGGAGGCTACTCTGCTTGGGTTGACAGTGGATTTGCTGGTGAGAAACCACCTGAAGAACTCAAAACAGCTTGCAAGTTCCGAGGTAGTTTATCATAAATTCCATATTTCAGGAAGTTAGAGTACTTCCAAGGAGTTTGTTGTAAGCTTGAACTAAGTGAACAGTGTTTGTAGTCTGTGTTGTGCTACAGAATAAATATTTAGTTCCAGCATTTCTGCAGGTTTCACTGTCTGTTGTACAAAAGAAAAAGTCTCGAAGTTCCAATTTCTTCTTCTTTCAGCTCTGA

mRNA sequence

ATGGAGAGGACAGTCGATGTTCAAGTTGCCAAAGATCTCTTAGAAAAGGGTCGCCTCTGTTTGGATGTCAGAACAGTTGAAGAGTATAACAAAGGCCATGTTGAGAATGCACTAAATGTTCCTTACGTGTTCTTCACCCCAGAAGTGGATTTGCTGGTGAGAAACCACCTGAAGAACTCAAAACAGCTTGCAAGTTCCGAGAATAAATATTTAGTTCCAGCATTTCTGCAGGTTTCACTGTCTGTTGTACAAAAGAAAAAGTCTCGAAGTTCCAATTTCTTCTTCTTTCAGCTCTGA

Coding sequence (CDS)

ATGGAGAGGACAGTCGATGTTCAAGTTGCCAAAGATCTCTTAGAAAAGGGTCGCCTCTGTTTGGATGTCAGAACAGTTGAAGAGTATAACAAAGGCCATGTTGAGAATGCACTAAATGTTCCTTACGTGTTCTTCACCCCAGAAGTGGATTTGCTGGTGAGAAACCACCTGAAGAACTCAAAACAGCTTGCAAGTTCCGAGAATAAATATTTAGTTCCAGCATTTCTGCAGGTTTCACTGTCTGTTGTACAAAAGAAAAAGTCTCGAAGTTCCAATTTCTTCTTCTTTCAGCTCTGA

Protein sequence

MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPEVDLLVRNHLKNSKQLASSENKYLVPAFLQVSLSVVQKKKSRSSNFFFFQL*
Homology
BLAST of Chy11G188480 vs. ExPASy Swiss-Prot
Match: F4IPI4 (Rhodanese-like domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=STR17 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.7e-08
Identity = 31/71 (43.66%), Postives = 43/71 (60.56%), Query Frame = 0

Query: 4   TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHV--ENALNVPYVFFTPEVDLLVRNHLKNSK 63
           T+DV  A+ LL+ G   LDVRTVEE+ KGHV  EN  NVPY  +TP+   +  N LK+  
Sbjct: 34  TIDVNQAQKLLDSGYTFLDVRTVEEFKKGHVDSENVFNVPYWLYTPQGQEINPNFLKHVS 93

Query: 64  QLASSENKYLV 73
            L +  +  ++
Sbjct: 94  SLCNQTDHLIL 104

BLAST of Chy11G188480 vs. ExPASy Swiss-Prot
Match: Q8RUD6 (Protein HIGH ARSENIC CONTENT 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HAC1 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 7.0e-07
Identity = 29/69 (42.03%), Postives = 38/69 (55.07%), Query Frame = 0

Query: 4   TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPEVDLLVRNHLKNSKQL 63
           TVDV  AK  L  G   LDVRT EE+ K HVE ALN+PY+F T E  ++  + L     +
Sbjct: 43  TVDVYTAKGFLSTGHRYLDVRTNEEFAKSHVEEALNIPYMFKTDEGRVINPDFLSQVASV 102

Query: 64  ASSENKYLV 73
              +   +V
Sbjct: 103 CKKDEHLIV 111

BLAST of Chy11G188480 vs. ExPASy Swiss-Prot
Match: Q9FKW8 (Thiosulfate sulfurtransferase 18 OS=Arabidopsis thaliana OX=3702 GN=STR18 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 6.5e-05
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 4  TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENA--LNVPYVFFTPEVDLLVRNHLKNSK 63
          +VDV  AK LL+ G   LDVRT +E+ +GH E A  +N+PY+  TP+  +  +  L+   
Sbjct: 16 SVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLEQVS 75

Query: 64 QLASSENKYLV 73
           L +  +  LV
Sbjct: 76 SLLNPADDILV 86

BLAST of Chy11G188480 vs. ExPASy Swiss-Prot
Match: Q39129 (Thiosulfate sulfurtransferase 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STR16 PE=1 SV=2)

HSP 1 Score: 45.1 bits (105), Expect = 5.5e-04
Identity = 22/40 (55.00%), Postives = 28/40 (70.00%), Query Frame = 0

Query: 4  TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYV 44
          +V V VA DLL  G   LDVRT EE+++GH   A+NVPY+
Sbjct: 10 SVSVTVAHDLLLAGHRYLDVRTPEEFSQGHACGAINVPYM 49

BLAST of Chy11G188480 vs. ExPASy TrEMBL
Match: A0A0A0LMB7 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270150 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 2.4e-18
Identity = 51/72 (70.83%), Postives = 56/72 (77.78%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPEVDLLVRNHLKNS 60
          MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE  +   + L   
Sbjct: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPEGQVKNPDFLAQV 60

Query: 61 KQLASSENKYLV 73
            +   E+  +V
Sbjct: 61 TSILKKEDHIVV 72

BLAST of Chy11G188480 vs. ExPASy TrEMBL
Match: A0A5D3BKL1 (Rhodanese-like domain-containing protein 19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001240 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.9e-15
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 49
          MERTVDVQVAKDLLEKG L LDVRTVEEYNKGHVENALNVPY+F TPE
Sbjct: 1  MERTVDVQVAKDLLEKGHLYLDVRTVEEYNKGHVENALNVPYMFLTPE 48

BLAST of Chy11G188480 vs. ExPASy TrEMBL
Match: A0A5A7TUB4 (Rhodanese-like domain-containing protein 19 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G002460 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.9e-15
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 49
          MERTVDVQVAKDLLEKG L LDVRTVEEYNKGHVENALNVPY+F TPE
Sbjct: 1  MERTVDVQVAKDLLEKGHLYLDVRTVEEYNKGHVENALNVPYMFLTPE 48

BLAST of Chy11G188480 vs. ExPASy TrEMBL
Match: A0A1S3C5M3 (rhodanese-like domain-containing protein 19, mitochondrial isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497320 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.9e-15
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 49
          MERTVDVQVAKDLLEKG L LDVRTVEEYNKGHVENALNVPY+F TPE
Sbjct: 1  MERTVDVQVAKDLLEKGHLYLDVRTVEEYNKGHVENALNVPYMFLTPE 48

BLAST of Chy11G188480 vs. ExPASy TrEMBL
Match: A0A1S3C7C4 (rhodanese-like domain-containing protein 19, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497320 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.9e-15
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 49
          MERTVDVQVAKDLLEKG L LDVRTVEEYNKGHVENALNVPY+F TPE
Sbjct: 1  MERTVDVQVAKDLLEKGHLYLDVRTVEEYNKGHVENALNVPYMFLTPE 48

BLAST of Chy11G188480 vs. NCBI nr
Match: XP_004147071.1 (protein HIGH ARSENIC CONTENT 1, mitochondrial [Cucumis sativus] >KGN61922.1 hypothetical protein Csa_006618 [Cucumis sativus])

HSP 1 Score: 101 bits (252), Expect = 3.36e-25
Identity = 48/48 (100.00%), Postives = 48/48 (100.00%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 48
          MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE
Sbjct: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 48

BLAST of Chy11G188480 vs. NCBI nr
Match: XP_008457677.1 (PREDICTED: rhodanese-like domain-containing protein 19, mitochondrial isoform X2 [Cucumis melo] >TYJ99554.1 rhodanese-like domain-containing protein 19 [Cucumis melo var. makuwa])

HSP 1 Score: 92.0 bits (227), Expect = 1.91e-21
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 48
          MERTVDVQVAKDLLEKG L LDVRTVEEYNKGHVENALNVPY+F TPE
Sbjct: 1  MERTVDVQVAKDLLEKGHLYLDVRTVEEYNKGHVENALNVPYMFLTPE 48

BLAST of Chy11G188480 vs. NCBI nr
Match: KAA0045726.1 (rhodanese-like domain-containing protein 19 [Cucumis melo var. makuwa])

HSP 1 Score: 92.0 bits (227), Expect = 1.96e-21
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 48
          MERTVDVQVAKDLLEKG L LDVRTVEEYNKGHVENALNVPY+F TPE
Sbjct: 1  MERTVDVQVAKDLLEKGHLYLDVRTVEEYNKGHVENALNVPYMFLTPE 48

BLAST of Chy11G188480 vs. NCBI nr
Match: XP_008457676.1 (PREDICTED: rhodanese-like domain-containing protein 19, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 92.0 bits (227), Expect = 2.36e-21
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 0

Query: 1  MERTVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 48
          MERTVDVQVAKDLLEKG L LDVRTVEEYNKGHVENALNVPY+F TPE
Sbjct: 1  MERTVDVQVAKDLLEKGHLYLDVRTVEEYNKGHVENALNVPYMFLTPE 48

BLAST of Chy11G188480 vs. NCBI nr
Match: XP_038901855.1 (protein HIGH ARSENIC CONTENT 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 85.9 bits (211), Expect = 6.20e-19
Identity = 40/45 (88.89%), Postives = 43/45 (95.56%), Query Frame = 0

Query: 4  TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPE 48
          TVDV+VAKDLL+KG L LDVRTVEEYNKGHVENALNVPY+FFTPE
Sbjct: 12 TVDVRVAKDLLQKGHLYLDVRTVEEYNKGHVENALNVPYMFFTPE 56

BLAST of Chy11G188480 vs. TAIR 10
Match: AT2G17850.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 2.6e-09
Identity = 31/71 (43.66%), Postives = 43/71 (60.56%), Query Frame = 0

Query: 4   TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHV--ENALNVPYVFFTPEVDLLVRNHLKNSK 63
           T+DV  A+ LL+ G   LDVRTVEE+ KGHV  EN  NVPY  +TP+   +  N LK+  
Sbjct: 34  TIDVNQAQKLLDSGYTFLDVRTVEEFKKGHVDSENVFNVPYWLYTPQGQEINPNFLKHVS 93

Query: 64  QLASSENKYLV 73
            L +  +  ++
Sbjct: 94  SLCNQTDHLIL 104

BLAST of Chy11G188480 vs. TAIR 10
Match: AT2G21045.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 5.0e-08
Identity = 29/69 (42.03%), Postives = 38/69 (55.07%), Query Frame = 0

Query: 4   TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENALNVPYVFFTPEVDLLVRNHLKNSKQL 63
           TVDV  AK  L  G   LDVRT EE+ K HVE ALN+PY+F T E  ++  + L     +
Sbjct: 43  TVDVYTAKGFLSTGHRYLDVRTNEEFAKSHVEEALNIPYMFKTDEGRVINPDFLSQVASV 102

Query: 64  ASSENKYLV 73
              +   +V
Sbjct: 103 CKKDEHLIV 111

BLAST of Chy11G188480 vs. TAIR 10
Match: AT5G66170.1 (sulfurtransferase 18 )

HSP 1 Score: 48.1 bits (113), Expect = 4.6e-06
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 4  TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENA--LNVPYVFFTPEVDLLVRNHLKNSK 63
          +VDV  AK LL+ G   LDVRT +E+ +GH E A  +N+PY+  TP+  +  +  L+   
Sbjct: 16 SVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLEQVS 75

Query: 64 QLASSENKYLV 73
           L +  +  LV
Sbjct: 76 SLLNPADDILV 86

BLAST of Chy11G188480 vs. TAIR 10
Match: AT5G66170.2 (sulfurtransferase 18 )

HSP 1 Score: 48.1 bits (113), Expect = 4.6e-06
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 4  TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENA--LNVPYVFFTPEVDLLVRNHLKNSK 63
          +VDV  AK LL+ G   LDVRT +E+ +GH E A  +N+PY+  TP+  +  +  L+   
Sbjct: 16 SVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLEQVS 75

Query: 64 QLASSENKYLV 73
           L +  +  LV
Sbjct: 76 SLLNPADDILV 86

BLAST of Chy11G188480 vs. TAIR 10
Match: AT5G66170.3 (sulfurtransferase 18 )

HSP 1 Score: 48.1 bits (113), Expect = 4.6e-06
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 4  TVDVQVAKDLLEKGRLCLDVRTVEEYNKGHVENA--LNVPYVFFTPEVDLLVRNHLKNSK 63
          +VDV  AK LL+ G   LDVRT +E+ +GH E A  +N+PY+  TP+  +  +  L+   
Sbjct: 16 SVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLEQVS 75

Query: 64 QLASSENKYLV 73
           L +  +  LV
Sbjct: 76 SLLNPADDILV 86

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IPI43.7e-0843.66Rhodanese-like domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=S... [more]
Q8RUD67.0e-0742.03Protein HIGH ARSENIC CONTENT 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FKW86.5e-0538.03Thiosulfate sulfurtransferase 18 OS=Arabidopsis thaliana OX=3702 GN=STR18 PE=1 S... [more]
Q391295.5e-0455.00Thiosulfate sulfurtransferase 16, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LMB72.4e-1870.83Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270150 P... [more]
A0A5D3BKL11.9e-1591.67Rhodanese-like domain-containing protein 19 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A5A7TUB41.9e-1591.67Rhodanese-like domain-containing protein 19 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C5M31.9e-1591.67rhodanese-like domain-containing protein 19, mitochondrial isoform X2 OS=Cucumis... [more]
A0A1S3C7C41.9e-1591.67rhodanese-like domain-containing protein 19, mitochondrial isoform X1 OS=Cucumis... [more]
Match NameE-valueIdentityDescription
XP_004147071.13.36e-25100.00protein HIGH ARSENIC CONTENT 1, mitochondrial [Cucumis sativus] >KGN61922.1 hypo... [more]
XP_008457677.11.91e-2191.67PREDICTED: rhodanese-like domain-containing protein 19, mitochondrial isoform X2... [more]
KAA0045726.11.96e-2191.67rhodanese-like domain-containing protein 19 [Cucumis melo var. makuwa][more]
XP_008457676.12.36e-2191.67PREDICTED: rhodanese-like domain-containing protein 19, mitochondrial isoform X1... [more]
XP_038901855.16.20e-1988.89protein HIGH ARSENIC CONTENT 1, mitochondrial [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G17850.12.6e-0943.66Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT2G21045.15.0e-0842.03Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT5G66170.14.6e-0638.03sulfurtransferase 18 [more]
AT5G66170.24.6e-0638.03sulfurtransferase 18 [more]
AT5G66170.34.6e-0638.03sulfurtransferase 18 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001763Rhodanese-like domainPFAMPF00581Rhodanesecoord: 9..48
e-value: 7.9E-7
score: 29.6
IPR001763Rhodanese-like domainPROSITEPS50206RHODANESE_3coord: 14..42
score: 9.639885
IPR036873Rhodanese-like domain superfamilyGENE3D3.40.250.10coord: 1..91
e-value: 3.6E-11
score: 45.0
IPR036873Rhodanese-like domain superfamilySUPERFAMILY52821Rhodanese/Cell cycle control phosphatasecoord: 3..63
NoneNo IPR availablePANTHERPTHR44542:SF14PROTEIN HIGH ARSENIC CONTENT 1, MITOCHONDRIAL-RELATEDcoord: 4..72
NoneNo IPR availableCDDcd00158RHODcoord: 10..42
e-value: 3.95425E-8
score: 44.5978
IPR044684Rhodanese-like domain-containing protein STR17/STR18/HARC1-likePANTHERPTHR44542THIOSULFATE SULFURTRANSFERASE 18coord: 4..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G188480.1Chy11G188480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity