Chy11G186800 (gene) Cucumber (hystrix) v1

Overview
NameChy11G186800
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionShikimate kinase
LocationchrH11: 1134557 .. 1139012 (+)
RNA-Seq ExpressionChy11G186800
SyntenyChy11G186800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGTAAGTATGGAGACTACGGTAACGCAGCAGTTGCAGTTTTCGCCATGGATTCACTCGAAGAGTATTTCTGCAAAATCGAAGGGTTCGCTGCATTTCCCTAGGAGATTAGCGGAGCAGGATGTATTGCAGGCCCCATTTTCTTTCGATTTACGAGTGTCCAGACATTCAAATCATCGGAGAACCGTCGCTTTGAAAATCTCCTGTTCTTATAAGAACTCTTCAGGTTTGGTATTAGCGATCTATTACTGATATATTCAATTTTCTCTACTTGAAGCAAATGAAATGAGATTACCTGTTAATTCGATGGTATTTTGTGGAATTATGTTTGTGTTTGGTAAGAGTCATTTATAGCTTAGCTCCTAGAATTTTTATTAGCATTTACGTGTGAAAATTTCTTTAATTCAGCTTTATCTACGTCATTGGAACCCCAATAATTCATGAAGTTCCTCATCTTTACCCATTCTAAAAATTAATGTTTCTTCACTTGTTCGAATTTGCTTGATATACTTAAACATCTTAGCACCACTTGTTAAATGTGTTTTAAAATGACAATTTTCATTTCAATCCGTTACCTTTTCTCCCAGTTGCTTTCACCGTGAAGACACATATAGTTGGTTTAAGATCTTTTTAGATGGGGGAGTCTAGAATTCGTGGAATAAGGAATTTTTTGAAAATATAGATCAGGAGTCTGAGTCTGATCAGTTTGATGCCTGAGGTAAGGGCCATAAAAACATTTTTCTTTCCTTTGAAACTATCCTATTGATTGGTTCCTGCCCAGAATGAGAGTTTCGTGAAATTTCCCTAATCATCAGCTTGAGTTTGATCCATTCTCCACATTTCTTCCTGCATAGAGACTATATTTGCAAAACTAATATTTCTAAAGTAGATAAAAAAGATAAAGAATAGAGAACAGGGTAATCGTCTTTTATGAACTTAAGATGTTAGGCCGACTGGGATATCTTCTATATTGTTGGGGATTTTGAGTGAGAGAACTAACATAATCTTCAGAGGACATAAGAATTTCTTTTCGCAATCCTGTGGTCCCTTGCTAGATTCTTTGTTTCGCTTTGGGCCTCTGTTTCTAAGCTCTTTTGTTACTATTAGCTTGGTCTTATTTCTCTTGATTGAAACTCCTTGGAGGTTGGCTCTTTTTTATGACATTTTTCTTTTTGTATGTTTGTGTACTCTTTCATTTTTTTTCCATGAAATTCGAGTTTTTTCAGGGAAAAAAGAACATTTTTGATAAAATTTTCAATTTTTGTGTTTCTCAGTGTTGGAATCTGGAAACCATTGTGCTTCTGTTGATGAGACTTTGGCTATACAGGTAAGGGTTTCCCAAACCCACCTTTTCATTATCATTTTACTAGGCTTTCCATCGCTTTCCTGCCTTCTCTGATTGTGGAGCCTCTTACTTTTCCTTTTCCCTGCTTTTTGCTAAAAGGGGGACGAAAGTTGTCCTATTAGTTGTTTAGCATATTTTTCATCTCTTTGCTCACTGGTCTTAAAATTCCAGAGGAAGTCCCGGGAGATTGAGTCATATTTAAATGGGCGCTGTATATTTCTTGTTGGTGAGTTTCTTTCTTGAAGTTCATCATTACTCTTGAGCTTATGCAGTTAGATATTTAAAATCTTATATATGAATGCAGGAATGATGGGCTCCGGAAAAACAACTGTGGGAAAAGTCTTGTCAAATGCACTTGGTTACTCCTTTTCGGATAGGTTTGTTTCATAGCTGTTAATGCATTTACTAACTTTCGACAAAATTCTTCTACTCTTAGTTACATCTTGATTTCATAGCAAGGGAAATGTCTACTTTCTTTAAGTTTTATAATTAAATTTAACTTCCAAGTTCTATTGAAAAGCAAAAGCTGCGTAAAATGCAGCTGACGTACATTATCACTGAAGCTGCAACAGGAGGAGAAAAATAACTAGATCCGGTTCCTAATTTTATTCCCATCTATAAATGAGAGAAAATAACTGTTGACCTAGGTTTTTATGCTAATGCTATGATTTCTGGCATCTGTATTGAATTTTGCTAGTCTATGCATAAGCACGTACTATCATTTGATGAGACATTTCCATGCTGATCTCTTGTGCTATTTTATGTTTTGGATGCCACAGTGATTCATTGGTGGAACAAGACATGGAAATTTCTGTAGCCGAAATTTTCAAGGTCTATGGGGAGGATTTCTTTAGAGAAAGGGAGGTCAGTCTTGATGCATTACTGCCAAATCATTTTTCACATCTTGGCATTATTTCTGTTGCATGTTCACTGTCATGTTCAAAGTTTATGTTGCATGTTCATTATTATGCTTACCTCTCTCTCTTTTTCTCAAGTGCACACACATTCATTATCTTTTCAAGCGTTTTAGCTGTTAGGTTTTATATCAGAACATGGATCTTGGCCGGGCTGCTCCTCTCTTCAATGATGAGGATATTTCAACTGTTTGTCTCCAATATACAATTGAATTTGTACTTCAGAGTACTCTTTAAAGTGTTATATGTAGTATCTAGAATTGACCAAAGTGATCTTATTGTAGATTTTCCTTTTTGTAGTTGAAGCAATTAGAGATTTTCATCTTGTATTGTTTAAGGGATTTGGTTCTTGCTTGAACCAAAACCACATATTAGCCTCGAAGGTCAAAGTTAATATCATTCCATTAATGATAATACCGTGAAAAGGTCGTTACTTTGTTTTTTTCCCCCTTCTTAAAGATAATACCGTGAAAAGGTCGTTATCACTTGCTAATAATAATTTCAACCTGTTCACTGGGCCATGAAATTTTTCCGTAGACTCAGAACGTGTAATGTTTCAAATTTCTCACTCTTGAAATATTGTTAGAACAAGACAAGCTCTATTTTATGCATATATGAAGTCCAAAACCGAACTTTAGCTGATTAACATACCAGTGAACCTAAACTTATCTTGATTCCTGCAGACGGAAGCATTGAGGAAGTTATCTTTGATGCGCCAGTTTGTTATTTCCACAGGCGGAGGTGCCGTAACGAGATCGATAAACTGGTAAGAAGACAGACTAGTTGAATACAAGTGATTCATTTCTAATTCGTTTTCAGTATCATTTGATAGAGTGAACTGTTTCTAACTTTTCAGGAAATATATGCATAAAGGCATCAGTGTTTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCAATTCTCGTCCCCTTTTGCATCATGATTCAACTGATGCATACTCAAAGGTATTTTTTTCCAGTGTTTGTTTCCCTTTATTCATAAATATGATCTTCCAGGTTTCTGCGAAATAATGTCCAATAACAAACGTTTTAAAATTAATTATGTTGAAGTTATTTTACACAACATAACATTAAATGTATGATTTTACAGAGTTTTTGTTTTCTCCTGACAACATAATTAGTAATAACCCTTAAAGATTAGTTTTAAAATTGACTCTCGAATAAATATTTTTCTATTTCCTGTTATCTACAGACCCTCGTCCGTTTATCTACTCTTCTGGACGAGAGGGGCGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAGTAAGACATCTCTCCTTGATATATTATTAATGTATGCTACCAACTTAATGTCGAAAACTCAATGGTTTTGGTTTTAATCTCATTCGACTACCGACCACTAAAGAGATGCCATTTAGGAAGAGTTTGAAATGAATTTTAGGATGTTAATTTTTCGCTTTAAACAGCTTTAGCATAGACACTTCATTAGTTTTTTTTTTTTTGTTATAGAAAATTGCTTTCATTTAAAGCTCTTTTTTCAAATGCCATTCCGAACAATTAAGTATTATTGGGTGGTTCCTCTATTCCTTGAATCTAAAATCTCAATACCAAATGACTTAAGCTAATGTTATCCTATGCAGAAATTGCAGCCAAACTGGGTACTAAAGACGTATCCAATGTCACGCCTATGGCCATAGCAATCGAGGTCTATAACTATATCTCTCATTCTCTGAATAAACATGATAACCTACGCACTACACCACAATTAACTAAAGCCTTTTAGACAACAAGTCTTATTTTATTTCTATAACAATTTTTTCCTCACACTTGTTTATACCATGGAATCTCAGGCACTCGAACAAATCGAGACCTTTTTGAAGAGAGAAGATGGTTATTGTGCATTTTGATGACCACAAAAAAGAAAAATGATTCATTTAGGTAATAACAGAAGCTTTCTTACTTCTCATAGCAGCAATGAAATGAAGTAGGTAGATGATCTTTTCATTTCATTTTGCTTATAGAGTTAATAATGTATGTTTATTTTTCTTATGATTTCAGGTACATATGAATAACGGCAATGAGTGGAAACATAATCGATTCTTTCATTATTTCTGGATAATCTTCTTTATCAAGTATCAAGGCATGTTTCAGTTTCACTCAAGGCTGTCCCATTTGTTCTCTTGA

mRNA sequence

ATGCCAGTAAGTATGGAGACTACGGTAACGCAGCAGTTGCAGTTTTCGCCATGGATTCACTCGAAGAGTATTTCTGCAAAATCGAAGGGTTCGCTGCATTTCCCTAGGAGATTAGCGGAGCAGGATGTATTGCAGGCCCCATTTTCTTTCGATTTACGAGTGTCCAGACATTCAAATCATCGGAGAACCGTCGCTTTGAAAATCTCCTGTTCTTATAAGAACTCTTCAGTGTTGGAATCTGGAAACCATTGTGCTTCTGTTGATGAGACTTTGGCTATACAGAGGAAGTCCCGGGAGATTGAGTCATATTTAAATGGGCGCTGTATATTTCTTGTTGGAATGATGGGCTCCGGAAAAACAACTGTGGGAAAAGTCTTGTCAAATGCACTTGGTTACTCCTTTTCGGATAGTGATTCATTGGTGGAACAAGACATGGAAATTTCTGTAGCCGAAATTTTCAAGGTCTATGGGGAGGATTTCTTTAGAGAAAGGGAGACGGAAGCATTGAGGAAGTTATCTTTGATGCGCCAGTTTGTTATTTCCACAGGCGGAGGTGCCGTAACGAGATCGATAAACTGGAAATATATGCATAAAGGCATCAGTGTTTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCAATTCTCGTCCCCTTTTGCATCATGATTCAACTGATGCATACTCAAAGACCCTCGTCCGTTTATCTACTCTTCTGGACGAGAGGGGCGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAAAATTGCAGCCAAACTGGGTACTAAAGACGTATCCAATGTCACGCCTATGGCCATAGCAATCGAGGTACATATGAATAACGGCAATGAGTGGAAACATAATCGATTCTTTCATTATTTCTGGATAATCTTCTTTATCAAGTATCAAGGCATGTTTCAGTTTCACTCAAGGCTGTCCCATTTGTTCTCTTGA

Coding sequence (CDS)

ATGCCAGTAAGTATGGAGACTACGGTAACGCAGCAGTTGCAGTTTTCGCCATGGATTCACTCGAAGAGTATTTCTGCAAAATCGAAGGGTTCGCTGCATTTCCCTAGGAGATTAGCGGAGCAGGATGTATTGCAGGCCCCATTTTCTTTCGATTTACGAGTGTCCAGACATTCAAATCATCGGAGAACCGTCGCTTTGAAAATCTCCTGTTCTTATAAGAACTCTTCAGTGTTGGAATCTGGAAACCATTGTGCTTCTGTTGATGAGACTTTGGCTATACAGAGGAAGTCCCGGGAGATTGAGTCATATTTAAATGGGCGCTGTATATTTCTTGTTGGAATGATGGGCTCCGGAAAAACAACTGTGGGAAAAGTCTTGTCAAATGCACTTGGTTACTCCTTTTCGGATAGTGATTCATTGGTGGAACAAGACATGGAAATTTCTGTAGCCGAAATTTTCAAGGTCTATGGGGAGGATTTCTTTAGAGAAAGGGAGACGGAAGCATTGAGGAAGTTATCTTTGATGCGCCAGTTTGTTATTTCCACAGGCGGAGGTGCCGTAACGAGATCGATAAACTGGAAATATATGCATAAAGGCATCAGTGTTTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCAATTCTCGTCCCCTTTTGCATCATGATTCAACTGATGCATACTCAAAGACCCTCGTCCGTTTATCTACTCTTCTGGACGAGAGGGGCGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAAAATTGCAGCCAAACTGGGTACTAAAGACGTATCCAATGTCACGCCTATGGCCATAGCAATCGAGGTACATATGAATAACGGCAATGAGTGGAAACATAATCGATTCTTTCATTATTTCTGGATAATCTTCTTTATCAAGTATCAAGGCATGTTTCAGTTTCACTCAAGGCTGTCCCATTTGTTCTCTTGA

Protein sequence

MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTVALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGKVLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEVHMNNGNEWKHNRFFHYFWIIFFIKYQGMFQFHSRLSHLFS*
Homology
BLAST of Chy11G186800 vs. ExPASy Swiss-Prot
Match: Q00497 (Shikimate kinase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=SK PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.9e-77
Identity = 155/284 (54.58%), Postives = 198/284 (69.72%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           ME  V+Q LQ S WI+S  +  K  G L F  +  E+   +   S  L+  + ++  R V
Sbjct: 1   MEARVSQSLQLSSWINSDKVVRKPSGLLRFSEKWNEKPRHRVVVSCHLQPRKAAHSDRRV 60

Query: 65  ALKISCSYKN--SSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTV 124
            LK+SCS +N  +SVLESG   AS+DE   ++ K+ E+E YL+GRC++LVGMMG GKTTV
Sbjct: 61  QLKVSCSPQNVQASVLESGCFSASIDEIETLKNKAEEVEEYLDGRCVYLVGMMGCGKTTV 120

Query: 125 GKVLSNALGYSFSDSDSLVEQDM-EISVAEIFKVYGEDFFRERETEALRKLSLMRQFVIS 184
           G++L+  LGYSF D D L+EQ +  I+VAEIF++ GE FFR+ ETE L KLSLM + V+S
Sbjct: 121 GRILAETLGYSFFDCDRLIEQAVGGITVAEIFELRGESFFRDNETEVLHKLSLMHRLVVS 180

Query: 185 TGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRL 244
           TGGGAV R INW++MHKGISVWLDVPLEAL KRI+  GT SRPLLH +S D Y  TL RL
Sbjct: 181 TGGGAVVRPINWRHMHKGISVWLDVPLEALAKRITTEGTKSRPLLHEESGDVYDTTLKRL 240

Query: 245 STLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEV 286
           +TL++ RGE YANA  +VS E IA K   KDV ++TP  I +EV
Sbjct: 241 TTLMETRGENYANASARVSLENIALK-REKDVCHITPAEITLEV 283

BLAST of Chy11G186800 vs. ExPASy Swiss-Prot
Match: Q9SJ05 (Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK1 PE=1 SV=2)

HSP 1 Score: 284.3 bits (726), Expect = 1.8e-75
Identity = 151/282 (53.55%), Postives = 197/282 (69.86%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAK-SKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRT 64
           ME  +TQ++Q+  W+  + +  K  +GSL + +++      +      L+  R ++ RR 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  VALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVG 124
           V+  +SCS  NSS L         DE + ++RK+ E++ YLNGR ++LVGMMGSGKTTVG
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPFDEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTVG 120

Query: 125 KVLSNALGYSFSDSDSLVEQDME-ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVIST 184
           K++S  LGY+F D D+L+EQ M   SVAEIF  +GE+FFR +ET+AL+KLS   Q V+ST
Sbjct: 121 KLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVST 180

Query: 185 GGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLS 244
           GGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AVGT+SRPLLH +S DAYS    RLS
Sbjct: 181 GGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLS 240

Query: 245 TLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
            + DERGEAY NA  +VS E IAAK G K+VS++TP  IAIE
Sbjct: 241 AIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIE 281

BLAST of Chy11G186800 vs. ExPASy Swiss-Prot
Match: Q8GY88 (Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK2 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.9e-69
Identity = 150/286 (52.45%), Postives = 196/286 (68.53%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           ME    Q+ Q+S W   ++   K +GSL +  +  ++D      +  L + +  +HR   
Sbjct: 1   MEAATVQRFQYSSWNDLRNFEGKPRGSLRYNTQRIKEDKRFRVVA--LTLDKRRDHRLR- 60

Query: 65  ALKISCSYKNSS-VLESGN--HCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTT 124
               S S KNSS +LE+G+  H    +E   +++K+ E++ YLNGR ++LVGMMGSGKTT
Sbjct: 61  ----SVSDKNSSALLETGSLLHSPFDEEQQILKKKAEEVKPYLNGRSMYLVGMMGSGKTT 120

Query: 125 VGKVLSNALGYSFSDSDSLVEQDME-ISVAEIFKVYGEDFFRERETEALRKLSLM-RQFV 184
           VGK+++ +LGY+F D D+L+EQ M+  SVAEIF+ +GE  FRE+ETEAL+KLSLM  Q V
Sbjct: 121 VGKIMARSLGYTFFDCDTLIEQAMKGTSVAEIFEHFGESVFREKETEALKKLSLMYHQVV 180

Query: 185 ISTGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHD-STDAYSKTL 244
           +STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AVGT SRPLLH D S D Y+  L
Sbjct: 181 VSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRPLLHDDESGDTYTAAL 240

Query: 245 VRLSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
            RLST+ D RGEAY  A  +VS E I  KLG + VS++TP  IAIE
Sbjct: 241 NRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAIE 279

BLAST of Chy11G186800 vs. ExPASy Swiss-Prot
Match: Q5NTH4 (Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK1 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.4e-64
Identity = 124/200 (62.00%), Postives = 156/200 (78.00%), Query Frame = 0

Query: 86  SVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGKVLSNALGYSFSDSDSLVEQDM 145
           SVDE L ++RKS E+  YLNGRCI+LVGMMGSGK+TVGK++S  LGYSF DSD LVEQ +
Sbjct: 76  SVDEALLLKRKSEEVLFYLNGRCIYLVGMMGSGKSTVGKIMSEVLGYSFFDSDKLVEQAV 135

Query: 146 EI-SVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 205
            + SVA+IFKV+ E FFR+ E+  LR LS M++ V++TGGGAV R +NWKYM KG+SVWL
Sbjct: 136 GMPSVAQIFKVHSEAFFRDNESSVLRDLSSMKRLVVATGGGAVIRPVNWKYMKKGLSVWL 195

Query: 206 DVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTLLDERGEAYANAEVKVSCEKI 265
           DVPL+AL +RI+ VGT SRPLL   S D Y+    +LS L ++RG+AYANA+V+VS E+I
Sbjct: 196 DVPLDALARRIAKVGTASRPLLDQPSGDPYTMAFSKLSMLAEQRGDAYANADVRVSLEEI 255

Query: 266 AAKLGTKDVSNVTPMAIAIE 285
           A+K G  DVS +TP  IAIE
Sbjct: 256 ASKQGHDDVSKLTPTDIAIE 275

BLAST of Chy11G186800 vs. ExPASy Swiss-Prot
Match: Q5NTH3 (Shikimate kinase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK2 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.6e-63
Identity = 123/200 (61.50%), Postives = 154/200 (77.00%), Query Frame = 0

Query: 86  SVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGKVLSNALGYSFSDSDSLVEQDM 145
           SVDE L ++RKS E+  YLNGRCI+LVGMMGSGK+TV K+L+  LGYSF DSD LVEQ +
Sbjct: 74  SVDEALLLKRKSEEVLFYLNGRCIYLVGMMGSGKSTVAKILAEVLGYSFFDSDKLVEQAV 133

Query: 146 EI-SVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 205
            + SVA+IFK + E FFR+ E+  LR LS MR+ V++TGGGAV R +NWKYM KG+SVWL
Sbjct: 134 GMPSVAQIFKEHSEAFFRDNESSVLRDLSSMRRLVVATGGGAVIRPVNWKYMKKGLSVWL 193

Query: 206 DVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTLLDERGEAYANAEVKVSCEKI 265
           DVPL+AL +RI+ VGT SRPLL   S+D Y+    +LS L ++RG+AYANA+ +VS E+I
Sbjct: 194 DVPLDALARRIAQVGTASRPLLDQPSSDPYTAAFSKLSMLAEQRGDAYANADARVSLEEI 253

Query: 266 AAKLGTKDVSNVTPMAIAIE 285
           AAK G  DVS +TP  IAIE
Sbjct: 254 AAKQGHDDVSKLTPTDIAIE 273

BLAST of Chy11G186800 vs. ExPASy TrEMBL
Match: A0A0A0LJX8 (Shikimate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G296130 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.2e-151
Identity = 278/284 (97.89%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60
           MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH
Sbjct: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60

Query: 61  RRTVALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKT 120
           RRTVALKISCSY NSSVLESGNHCASVDETLAIQRKSREIESYLNGRCI+LVGMMGSGKT
Sbjct: 61  RRTVALKISCSYNNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIYLVGMMGSGKT 120

Query: 121 TVGKVLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVI 180
           TVGKVLSNALGYSFSDSDSLVEQDM ISVAE+FKVYGEDFFRERETEALRKLSLMRQFVI
Sbjct: 121 TVGKVLSNALGYSFSDSDSLVEQDMGISVAEVFKVYGEDFFRERETEALRKLSLMRQFVI 180

Query: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVR 240
           STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDS DAYSKTLVR
Sbjct: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVR 240

Query: 241 LSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
           LSTLL+ERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284

BLAST of Chy11G186800 vs. ExPASy TrEMBL
Match: E7BBT4 (Shikimate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.9e-151
Identity = 277/284 (97.54%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60
           MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH
Sbjct: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60

Query: 61  RRTVALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKT 120
           RRTVALKISCSY NSSVLESGNHCASVDETLAIQRKSREIESYLNGRCI+LVGMMGSGKT
Sbjct: 61  RRTVALKISCSYNNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIYLVGMMGSGKT 120

Query: 121 TVGKVLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVI 180
           TVGKVLSNALGYSFSDSDSLVEQDM ISVAE+FKVYGEDFFRERETEAL KLSLMRQFVI
Sbjct: 121 TVGKVLSNALGYSFSDSDSLVEQDMGISVAEVFKVYGEDFFRERETEALGKLSLMRQFVI 180

Query: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVR 240
           STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDS DAYSKTLVR
Sbjct: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVR 240

Query: 241 LSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
           LSTLL+ERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284

BLAST of Chy11G186800 vs. ExPASy TrEMBL
Match: A0A1S3C781 (Shikimate kinase OS=Cucumis melo OX=3656 GN=LOC103497472 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 9.9e-146
Identity = 268/280 (95.71%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           METTV QQLQFSPWIHSKS+SAK KGSLHFPRRLAEQD+LQAPFSFDLRVSRHSNHRRTV
Sbjct: 1   METTVMQQLQFSPWIHSKSVSAKPKGSLHFPRRLAEQDILQAPFSFDLRVSRHSNHRRTV 60

Query: 65  ALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGK 124
           ALKISCSYKNSSVLESGNHCASVDE+LAIQRKSREIESYLNGR I+LVGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHCASVDESLAIQRKSREIESYLNGRSIYLVGMMGSGKTTVGK 120

Query: 125 VLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 184
           VLSNALGYSFSDSDSLVE+D  ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG
Sbjct: 121 VLSNALGYSFSDSDSLVEEDTGISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 180

Query: 185 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 244
           GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL
Sbjct: 181 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 240

Query: 245 LDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
           L+ERGEAYANAEV VSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LEERGEAYANAEVTVSCEKIAAKLGTKDVSNVTPMAIAIE 280

BLAST of Chy11G186800 vs. ExPASy TrEMBL
Match: A0A5A7TS61 (Shikimate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002170 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 9.9e-146
Identity = 268/280 (95.71%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           METTV QQLQFSPWIHSKS+SAK KGSLHFPRRLAEQD+LQAPFSFDLRVSRHSNHRRTV
Sbjct: 1   METTVMQQLQFSPWIHSKSVSAKPKGSLHFPRRLAEQDILQAPFSFDLRVSRHSNHRRTV 60

Query: 65  ALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGK 124
           ALKISCSYKNSSVLESGNHCASVDE+LAIQRKSREIESYLNGR I+LVGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHCASVDESLAIQRKSREIESYLNGRSIYLVGMMGSGKTTVGK 120

Query: 125 VLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 184
           VLSNALGYSFSDSDSLVE+D  ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG
Sbjct: 121 VLSNALGYSFSDSDSLVEEDTGISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 180

Query: 185 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 244
           GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL
Sbjct: 181 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 240

Query: 245 LDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
           L+ERGEAYANAEV VSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LEERGEAYANAEVTVSCEKIAAKLGTKDVSNVTPMAIAIE 280

BLAST of Chy11G186800 vs. ExPASy TrEMBL
Match: A0A6J1I3Z2 (Shikimate kinase OS=Cucurbita maxima OX=3661 GN=LOC111470366 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.9e-135
Identity = 250/280 (89.29%), Postives = 264/280 (94.29%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           ME T+TQQLQFSPWIHSKSI+AK KGSLHF RRL EQ +LQAPFSF+L++SR S+H RTV
Sbjct: 1   MEATLTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRTV 60

Query: 65  ALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGK 124
           ALKISCSYKNSSVLESGNH   VDET  IQRKSREIESYLNGRCI+ VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHRVPVDETFTIQRKSREIESYLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 184
           VLSNALGYSFSDSDSLVEQD+ ISVAEIFKVYGEDFFRERETEALRKLSLM QFVISTGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 244
           GAVTR+INWKYM +GISVWLDVPLEALVKRISAVGTNSRPLLH+DSTDAYSKTLVRLSTL
Sbjct: 181 GAVTRTINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLVRLSTL 240

Query: 245 LDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
           L+ERGEAYANAEV+VSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIE 280

BLAST of Chy11G186800 vs. NCBI nr
Match: XP_011649381.1 (shikimate kinase, chloroplastic isoform X1 [Cucumis sativus] >XP_011649382.1 shikimate kinase, chloroplastic isoform X1 [Cucumis sativus] >KGN62088.1 hypothetical protein Csa_006505 [Cucumis sativus])

HSP 1 Score: 546 bits (1406), Expect = 2.05e-194
Identity = 278/284 (97.89%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60
           MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH
Sbjct: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60

Query: 61  RRTVALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKT 120
           RRTVALKISCSY NSSVLESGNHCASVDETLAIQRKSREIESYLNGRCI+LVGMMGSGKT
Sbjct: 61  RRTVALKISCSYNNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIYLVGMMGSGKT 120

Query: 121 TVGKVLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVI 180
           TVGKVLSNALGYSFSDSDSLVEQDM ISVAE+FKVYGEDFFRERETEALRKLSLMRQFVI
Sbjct: 121 TVGKVLSNALGYSFSDSDSLVEQDMGISVAEVFKVYGEDFFRERETEALRKLSLMRQFVI 180

Query: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVR 240
           STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDS DAYSKTLVR
Sbjct: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVR 240

Query: 241 LSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284
           LSTLL+ERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284

BLAST of Chy11G186800 vs. NCBI nr
Match: NP_001292691.1 (shikimate kinase, chloroplastic [Cucumis sativus] >CBY85385.1 shikimate kinase [Cucumis sativus])

HSP 1 Score: 543 bits (1399), Expect = 2.38e-193
Identity = 277/284 (97.54%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60
           MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH
Sbjct: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60

Query: 61  RRTVALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKT 120
           RRTVALKISCSY NSSVLESGNHCASVDETLAIQRKSREIESYLNGRCI+LVGMMGSGKT
Sbjct: 61  RRTVALKISCSYNNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIYLVGMMGSGKT 120

Query: 121 TVGKVLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVI 180
           TVGKVLSNALGYSFSDSDSLVEQDM ISVAE+FKVYGEDFFRERETEAL KLSLMRQFVI
Sbjct: 121 TVGKVLSNALGYSFSDSDSLVEQDMGISVAEVFKVYGEDFFRERETEALGKLSLMRQFVI 180

Query: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVR 240
           STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDS DAYSKTLVR
Sbjct: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVR 240

Query: 241 LSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284
           LSTLL+ERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284

BLAST of Chy11G186800 vs. NCBI nr
Match: XP_008457898.1 (PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo] >XP_008457899.1 PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo] >KAA0045894.1 shikimate kinase [Cucumis melo var. makuwa] >TYK13694.1 shikimate kinase [Cucumis melo var. makuwa])

HSP 1 Score: 526 bits (1355), Expect = 1.04e-186
Identity = 268/280 (95.71%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           METTV QQLQFSPWIHSKS+SAK KGSLHFPRRLAEQD+LQAPFSFDLRVSRHSNHRRTV
Sbjct: 1   METTVMQQLQFSPWIHSKSVSAKPKGSLHFPRRLAEQDILQAPFSFDLRVSRHSNHRRTV 60

Query: 65  ALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGK 124
           ALKISCSYKNSSVLESGNHCASVDE+LAIQRKSREIESYLNGR I+LVGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHCASVDESLAIQRKSREIESYLNGRSIYLVGMMGSGKTTVGK 120

Query: 125 VLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 184
           VLSNALGYSFSDSDSLVE+D  ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG
Sbjct: 121 VLSNALGYSFSDSDSLVEEDTGISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 180

Query: 185 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 244
           GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL
Sbjct: 181 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 240

Query: 245 LDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284
           L+ERGEAYANAEV VSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LEERGEAYANAEVTVSCEKIAAKLGTKDVSNVTPMAIAIE 280

BLAST of Chy11G186800 vs. NCBI nr
Match: XP_038902715.1 (shikimate kinase, chloroplastic-like [Benincasa hispida] >XP_038902716.1 shikimate kinase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 504 bits (1298), Expect = 4.99e-178
Identity = 259/284 (91.20%), Postives = 271/284 (95.42%), Query Frame = 0

Query: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60
           MP SMETTVTQQLQFSPWIHSKSI+AK KG LHF RRLAEQ    +PFSFDL+VSRHS+ 
Sbjct: 1   MPASMETTVTQQLQFSPWIHSKSIAAKPKGLLHFSRRLAEQ----SPFSFDLQVSRHSSQ 60

Query: 61  RRTVALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKT 120
           RRTVALKISCSYKNSSVLESGNHCASVDE+LAIQRKSREIESYLNGRCI+LVGMMGSGKT
Sbjct: 61  RRTVALKISCSYKNSSVLESGNHCASVDESLAIQRKSREIESYLNGRCIYLVGMMGSGKT 120

Query: 121 TVGKVLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVI 180
           TVGKVLSNALGYSFSDSDSLVEQD+ ISVAEIFKVYGEDFFRERETEALRKLSLM QFVI
Sbjct: 121 TVGKVLSNALGYSFSDSDSLVEQDIGISVAEIFKVYGEDFFRERETEALRKLSLMHQFVI 180

Query: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVR 240
           STGGGAVTR+INWKYMHKGISVWLDVPLEALVKRIS VGT+SRPLLHHDSTDAY+KTLVR
Sbjct: 181 STGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISVVGTSSRPLLHHDSTDAYTKTLVR 240

Query: 241 LSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284
           LSTLL+ERGEAYANA+V VSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LSTLLEERGEAYANAKVTVSCEKIAAKLGTKDVSNVTPMAIAIE 280

BLAST of Chy11G186800 vs. NCBI nr
Match: XP_023512754.1 (shikimate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023512755.1 shikimate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 494 bits (1271), Expect = 6.45e-174
Identity = 252/280 (90.00%), Postives = 266/280 (95.00%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           ME TVTQQLQFSPWIHSKSI+AK KGSLHF RRL EQ +LQAPFSF+L++SR S+H RTV
Sbjct: 1   MEATVTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRTV 60

Query: 65  ALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVGK 124
           ALKISCSYKNSSVLESGNH  SVDETL IQRKSREIESYLNGRCI+ VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHRVSVDETLIIQRKSREIESYLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSNALGYSFSDSDSLVEQDMEISVAEIFKVYGEDFFRERETEALRKLSLMRQFVISTGG 184
           VLSNALGYSFSDSDSLVEQD+ ISVAEIFKVYGEDFFRERETEALRKLSLM QFVISTGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTL 244
           GAVTR+INWKYM +GISVWLDVPLEALVKRISAVGTNSRPLLH+DSTDAYSKTL+RLSTL
Sbjct: 181 GAVTRTINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLIRLSTL 240

Query: 245 LDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 284
           L+ERGEAYANAEV+VSCEKIAAKLGTKDVSNVTPMAIAIE
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIE 280

BLAST of Chy11G186800 vs. TAIR 10
Match: AT2G21940.1 (shikimate kinase 1 )

HSP 1 Score: 284.3 bits (726), Expect = 1.3e-76
Identity = 151/282 (53.55%), Postives = 197/282 (69.86%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAK-SKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRT 64
           ME  +TQ++Q+  W+  + +  K  +GSL + +++      +      L+  R ++ RR 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  VALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVG 124
           V+  +SCS  NSS L         DE + ++RK+ E++ YLNGR ++LVGMMGSGKTTVG
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPFDEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTVG 120

Query: 125 KVLSNALGYSFSDSDSLVEQDME-ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVIST 184
           K++S  LGY+F D D+L+EQ M   SVAEIF  +GE+FFR +ET+AL+KLS   Q V+ST
Sbjct: 121 KLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVST 180

Query: 185 GGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLS 244
           GGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AVGT+SRPLLH +S DAYS    RLS
Sbjct: 181 GGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLS 240

Query: 245 TLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
            + DERGEAY NA  +VS E IAAK G K+VS++TP  IAIE
Sbjct: 241 AIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIE 281

BLAST of Chy11G186800 vs. TAIR 10
Match: AT2G21940.2 (shikimate kinase 1 )

HSP 1 Score: 284.3 bits (726), Expect = 1.3e-76
Identity = 151/282 (53.55%), Postives = 197/282 (69.86%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAK-SKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRT 64
           ME  +TQ++Q+  W+  + +  K  +GSL + +++      +      L+  R ++ RR 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  VALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVG 124
           V+  +SCS  NSS L         DE + ++RK+ E++ YLNGR ++LVGMMGSGKTTVG
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPFDEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTVG 120

Query: 125 KVLSNALGYSFSDSDSLVEQDME-ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVIST 184
           K++S  LGY+F D D+L+EQ M   SVAEIF  +GE+FFR +ET+AL+KLS   Q V+ST
Sbjct: 121 KLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVST 180

Query: 185 GGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLS 244
           GGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AVGT+SRPLLH +S DAYS    RLS
Sbjct: 181 GGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLS 240

Query: 245 TLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
            + DERGEAY NA  +VS E IAAK G K+VS++TP  IAIE
Sbjct: 241 AIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIE 281

BLAST of Chy11G186800 vs. TAIR 10
Match: AT2G21940.5 (shikimate kinase 1 )

HSP 1 Score: 284.3 bits (726), Expect = 1.3e-76
Identity = 151/282 (53.55%), Postives = 197/282 (69.86%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAK-SKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRT 64
           ME  +TQ++Q+  W+  + +  K  +GSL + +++      +      L+  R ++ RR 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  VALKISCSYKNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTTVG 124
           V+  +SCS  NSS L         DE + ++RK+ E++ YLNGR ++LVGMMGSGKTTVG
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPFDEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTVG 120

Query: 125 KVLSNALGYSFSDSDSLVEQDME-ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVIST 184
           K++S  LGY+F D D+L+EQ M   SVAEIF  +GE+FFR +ET+AL+KLS   Q V+ST
Sbjct: 121 KLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVST 180

Query: 185 GGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLS 244
           GGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AVGT+SRPLLH +S DAYS    RLS
Sbjct: 181 GGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLS 240

Query: 245 TLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
            + DERGEAY NA  +VS E IAAK G K+VS++TP  IAIE
Sbjct: 241 AIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIE 281

BLAST of Chy11G186800 vs. TAIR 10
Match: AT2G21940.4 (shikimate kinase 1 )

HSP 1 Score: 282.3 bits (721), Expect = 4.8e-76
Identity = 153/284 (53.87%), Postives = 202/284 (71.13%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAK-SKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRT 64
           ME  +TQ++Q+  W+  + +  K  +GSL + +++      +      L+  R ++ RR 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  VALKISCSYKNSS--VLESGNHCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTT 124
           V+  +SCS  NSS  +LE+G+     DE + ++RK+ E++ YLNGR ++LVGMMGSGKTT
Sbjct: 61  VSPAVSCSDNNSSGALLETGS-VYPFDEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTT 120

Query: 125 VGKVLSNALGYSFSDSDSLVEQDME-ISVAEIFKVYGEDFFRERETEALRKLSLMRQFVI 184
           VGK++S  LGY+F D D+L+EQ M   SVAEIF  +GE+FFR +ET+AL+KLS   Q V+
Sbjct: 121 VGKLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVV 180

Query: 185 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSTDAYSKTLVR 244
           STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AVGT+SRPLLH +S DAYS    R
Sbjct: 181 STGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKR 240

Query: 245 LSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
           LS + DERGEAY NA  +VS E IAAK G K+VS++TP  IAIE
Sbjct: 241 LSAIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIE 282

BLAST of Chy11G186800 vs. TAIR 10
Match: AT4G39540.1 (shikimate kinase 2 )

HSP 1 Score: 264.2 bits (674), Expect = 1.4e-70
Identity = 150/286 (52.45%), Postives = 196/286 (68.53%), Query Frame = 0

Query: 5   METTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNHRRTV 64
           ME    Q+ Q+S W   ++   K +GSL +  +  ++D      +  L + +  +HR   
Sbjct: 1   MEAATVQRFQYSSWNDLRNFEGKPRGSLRYNTQRIKEDKRFRVVA--LTLDKRRDHRLR- 60

Query: 65  ALKISCSYKNSS-VLESGN--HCASVDETLAIQRKSREIESYLNGRCIFLVGMMGSGKTT 124
               S S KNSS +LE+G+  H    +E   +++K+ E++ YLNGR ++LVGMMGSGKTT
Sbjct: 61  ----SVSDKNSSALLETGSLLHSPFDEEQQILKKKAEEVKPYLNGRSMYLVGMMGSGKTT 120

Query: 125 VGKVLSNALGYSFSDSDSLVEQDME-ISVAEIFKVYGEDFFRERETEALRKLSLM-RQFV 184
           VGK+++ +LGY+F D D+L+EQ M+  SVAEIF+ +GE  FRE+ETEAL+KLSLM  Q V
Sbjct: 121 VGKIMARSLGYTFFDCDTLIEQAMKGTSVAEIFEHFGESVFREKETEALKKLSLMYHQVV 180

Query: 185 ISTGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHD-STDAYSKTL 244
           +STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AVGT SRPLLH D S D Y+  L
Sbjct: 181 VSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRPLLHDDESGDTYTAAL 240

Query: 245 VRLSTLLDERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 285
            RLST+ D RGEAY  A  +VS E I  KLG + VS++TP  IAIE
Sbjct: 241 NRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAIE 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q004971.9e-7754.58Shikimate kinase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=SK PE=1 SV=1[more]
Q9SJ051.8e-7553.55Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK1 PE=1 SV... [more]
Q8GY881.9e-6952.45Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK2 PE=1 SV... [more]
Q5NTH45.4e-6462.00Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK... [more]
Q5NTH34.6e-6361.50Shikimate kinase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK... [more]
Match NameE-valueIdentityDescription
A0A0A0LJX81.2e-15197.89Shikimate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G296130 PE=3 SV=1[more]
E7BBT47.9e-15197.54Shikimate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A1S3C7819.9e-14695.71Shikimate kinase OS=Cucumis melo OX=3656 GN=LOC103497472 PE=3 SV=1[more]
A0A5A7TS619.9e-14695.71Shikimate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002... [more]
A0A6J1I3Z27.9e-13589.29Shikimate kinase OS=Cucurbita maxima OX=3661 GN=LOC111470366 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011649381.12.05e-19497.89shikimate kinase, chloroplastic isoform X1 [Cucumis sativus] >XP_011649382.1 shi... [more]
NP_001292691.12.38e-19397.54shikimate kinase, chloroplastic [Cucumis sativus] >CBY85385.1 shikimate kinase [... [more]
XP_008457898.11.04e-18695.71PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo] >XP_008457899.1 P... [more]
XP_038902715.14.99e-17891.20shikimate kinase, chloroplastic-like [Benincasa hispida] >XP_038902716.1 shikima... [more]
XP_023512754.16.45e-17490.00shikimate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023512755.... [more]
Match NameE-valueIdentityDescription
AT2G21940.11.3e-7653.55shikimate kinase 1 [more]
AT2G21940.21.3e-7653.55shikimate kinase 1 [more]
AT2G21940.51.3e-7653.55shikimate kinase 1 [more]
AT2G21940.44.8e-7653.87shikimate kinase 1 [more]
AT4G39540.11.4e-7052.45shikimate kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01100SHIKIMTKNASEcoord: 109..124
score: 62.02
coord: 133..146
score: 32.42
coord: 199..216
score: 34.62
coord: 161..169
score: 53.85
coord: 179..188
score: 66.15
NoneNo IPR availablePANTHERPTHR21087SHIKIMATE KINASEcoord: 5..289
NoneNo IPR availablePANTHERPTHR21087:SF20SHIKIMATE KINASE 1, CHLOROPLASTIC-LIKE ISOFORM X1coord: 5..289
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 91..296
e-value: 4.2E-64
score: 218.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 107..232
IPR031322Shikimate kinase/gluconokinasePFAMPF01202SKIcoord: 115..267
e-value: 1.5E-43
score: 148.7
IPR023000Shikimate kinase, conserved sitePROSITEPS01128SHIKIMATE_KINASEcoord: 162..187
IPR000623Shikimate kinase/Threonine synthase-like 1HAMAPMF_00109Shikimate_kinasecoord: 106..278
score: 24.053249
IPR000623Shikimate kinase/Threonine synthase-like 1CDDcd00464SKcoord: 109..268
e-value: 1.59019E-54
score: 173.511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G186800.1Chy11G186800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008652 cellular amino acid biosynthetic process
biological_process GO:0009423 chorismate biosynthetic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0009507 chloroplast
molecular_function GO:0005524 ATP binding
molecular_function GO:0004765 shikimate kinase activity