Chy10G184220 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G184220
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Uricase
Location	chrH10: 16935443 .. 16941388 (+)
RNA-Seq Expression	Chy10G184220
Synteny	Chy10G184220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCCCGACGACGCTGTAGTCGTAGTGAACGGCTTGAACTTTGAGCAGAGGCACGGCAAGGAACGAGTGCGAGTGGCTCGGGTTTGGCGGACCAAAGGCGGCCGTCATTTTATTGTTGAATGGAGCGTTGGCATCAGTCTTCTCTCCGATTGCGTCGCTGCATACGTTAGCGATGATAATTCCGATATTGTTGCAACCGATACTATGAAGAACACTGTAATTGTCTTCTAATATACTGTTTGTTGCATGATGATTTTATTGTTGCGGCTCTTGTGAATTTGATTGTTTTGTGAGTGTTGGAAGAGGAAAAAGGAAAGTGAAAAGTAGTATTGTTTTTTTGTGTGATTTTTTTGACTTGGTGTGTTTGGTTGAGTTATTAGTTGCATTAGTATTATTTAGTGGTAGAACATGTTCATTGCTTATGTGGGTATTGAAATTTTTATTGCTTAACTTAGACCAATCGCGTAGGATCTGGAAGTTTTTATTCGTATGGAAAAGAATTCTGCTGCTCAGCTCTCATCTTTTTTTTTTCCCTTTACTATTTACTTTATTGGATCGTTCTATGGTGTGCTGCTTTGAGTGAAAATGATATCGAGCAACTTCTGATTTTGAAAATTTTACCAAATTAGTTAAGCAGGTCGTCAGTGGAAGATTTATGGAGCCACCGTTTGGATCTTGAATTTGGGAAACAGGGCAAATCGCAAAATTAGTTTACGTTAGGGATTACAAAAAGTGAGGGATTTGTTTGACTGGGGGGATAAAGATGGGAAAGTTGTTTTAGATGCCGTTCGTCAGGACTATCCTAAGACTATAATAACCACAACTTGCTATGCTAAATACTATTTTGTATTTTCCAATTTCTATTTGATACATTTCTTACTATTTTACCTTCATTATCTTTTTTTATTATTTGCTACAGTATATATACTATTTCATTCTCAAGCTAAAATAGTTTAGACCTCAAATATATACTATTATAACTCAAACTAAAATAATACATACAGTAAGTGCAAACTATTATAATAACCCAATACTATAATAACCAACTTCCTTCTTACCCCAAACGTTCCCTAGAAGTCAAAACTTTGTTATGTGGAAACAGAGTGGGAAGGAAATCTTGTGGACTATCTTACCTTTTTTCGTCTTAATCATAGCAGATCATATTTGCATTTTTTGGTTCTCCCACGTTTATTTCCTTTTGTTACAGTTTTAGATTTTAATACTGTTACTCCCTTTAATGTTAAAAGTTAATTGATTTTATTTCTTCAGGTATATGCAAAAGCCAAGGAGTGTTCTGACCAAACTTCAGTGGAAGATTTTGCAATTTTTCTTGCCAAGCACTTCACGTTATGTTACAGTCAGGTAAACCGCATGTTTAAACTTGTATTCTTAACGCTATGCAGGTCAGTATGTTGACACATATGCAGGTCGAGACTTGGTGAAGTTGTAGGTTGGATTTGTGGTAAATCAGAGGTTGTGATTTGGTGTGAGTTTTAATGGGTGTAAAGTGAACATAGTTTAGCTGTATTAATGTGCATGACTTTTATCTACTCAGTATACTCACATTGCATCAACCAAAATAACTTCTCGCCTGCTCCATATCCACCACTACATGGGGCTGTTATGAATTAAAAGTACAAGTTATCTCCATTTCAAAACACTTACATGATCATCTAACCACTTTTAGGACTTCATTTTAACTCAAATGTTTTTTCCGGAGGTTGCAAGATCCATACCCTTCAATATTGAAGTTATGTTACTTTTCTCATTGAAACTAACTTGGCTGCCTGCACCGAAGTATACATAATTTGAGGGTGTTTTTTTTTTGGTGGATTACCTACCTGCTTTCTGAATCAAAAACCTTGTTCTTCATGTGGTAAATGAAGAAAAAAAGTGCTCAGTCTGCTATTCTAGATCTCTTGCTTTAGACTATATATATATATATATTAATATTACAAACATGCACCAATATGACCATAGCATTTTAGTTTTCCTCCACATCTATATGCCCATTGAGATGTTTTCCAGATGCATGCACTACTGATTAATTATCAGTCCACTGAATTAATTGCATATAGCCAGGATAGAGTAAACACCTACATACCACGAACTTTCACAAAAGGATTTCCTGCTTCCTTCAAACTTATTTATTTTTCGTCTTATCTTCTGCTCAAATCCCTTTTCCTCCTCATTGTACACATGCACACTTTGGGTTCTTTTTTTATTAGTTGACGCTGAAAGTTAATAAATCCAAAGATGTTCTCCAATATGTATAATTAATTTGCAGGTCACTACTACCATAGTGAAGATTGCAGAAAAGTCATGGGAGCGTGTATCTGTCAATGGTCAACCACACAACCATGGTTAGCTGTTCATTTTTGGAAAGGCTGATTCTGAGAATTGACTTTTATCATTATATTCAACCTTTTCAATGTTGAAATATTTAGTTTAAATAACTTAAAAGGGGTTTAAATGATCGTGAATTTTGTTTAACTTTATTTCTTGAAACCCTTCTCAAGTAAGTTCTCGTGTGCCTCTCATGATGATAATAAGGATTTGGATCCTTGGCGAGAGTCTTCTGGTGTTAAAGTCAACAAGAATTTTTTTTAATAGGGTGAAAATTTTCTGTTCAGCATAACTAAAGATAAATTGATTAGAGGGATATCCACGAATTTTAACATACACAACCGTATCTTTTAGTTTTTACAATGTGTGCAAAATTCTTAACATTCGTCTCTATGGACTTCCAGTTATAAGTTTTTTACTACGGAATAAGAAATCACTTAGACTTGAAATTTTGTATCTTAGACTTTTGTTTGTGGAGCAGAAGGCTTATTCCAGTCAATTTTTCTGTACATCTCTTGCTTTAATGCACATATTTTGGTAAACTATGTATCTATTTTAACATAATAGAATTGGGCATGTGCGTATTTTTTCAGAATCTAAATAACAGCTACTTGTTTCCCGAGTTCTTATTTTTATCCTTATTTTCTTCTTTAATAACTTTCGTTTGAAGGCTTCAAACTTGGATCTGAAAAGCACACAACAGAGGTGATATTTAAGAAGTCAGGTGTTTTACAAGTGAGCTCTGGTATTGAAGGACTATCTTTGCTGAAGACAACTCAAGTAAGTCTACTCTTTTATATACATATTTTTTGAACTCAAGGACTTAATTGTACAATCTACTGTGCAACTAAGATCCTCTCTTTTTAATACACTTTCAAAATCCATTTCATGAACTATTTATGTTTTTGTCGATGCTAAATATAACAGATCTCCTGACTGTTTCTAAACTATGCATATTTACTGCTTTCTCACTTTGTCAATGCATTCATTATCTAGTGACCACAAATGTCTTTCTGGACTATTCTTTATAAGTACACGTTTTGCTCATATATTCTAACTCAATATACTTTAAGTATCTCCTAAAAATAATTCATGTAGAATATTTTATTACTTGATAGTTGATACTTCTAGAAATTGAGCTTTTTGTTTGGACTAGGCCCTGGTACTTCTAACCCAAAATCAAGTTTATATTTCTAACACTTTTCTTATTTTGGATCTGGCCTCATCTATTTTTGTAATATTGAAACTATTTTATTTTCTCCATCAGTCAATGAAGCCACTTCAGCATCGTCTACCTAATTTGAACAATTGTTTTGAATAATTTATCTTTATTCTTGATCAGCATTAAACAATTCGAAACCTGAACCCCACCCTTCATATTCTAACCTATTCAAGCTTAAGGAGAAGTACCCTGAAATTGTCCAAGGGCACTCAGTAGTGTCCATAGTTAGAAATTGTTCGTATGTTCAGGCAATATGAGCAAATAATTTGAGATATATCCGACAATTTGAAAACTACAGAAATAGTTTTCAAACACTCGTTTTTCGTTTCTAGAATTTGACATGAAAACCATGATTAAGAAATTGTGAGAAAACAATCCTAGTTTCCCAAATGATACCAAATTGATATAACTTAAGGAGGATGGTGTATCCTGGGGTAACATCTTCTAATGAAGAGTTGGCATTTGATCAACTGGTAGAATGCATAGCAGATGAAAATTTTAAAAAACTTAATACTTGTAGATGCGGTTTAGATATATTATGTTTCTCCTGATTCTTTCTCTAAACTACATCGTGCAAGCAGTCAGGTTTTGAGCGATTTCATAGGGATAAGTACACAGCTCTTCCTGAGACACGAGAGAGGATATTAGCCACTGAAGTATCTGCTTCATGGAGGTAAATTTTCTATCAGTCGGACTTTTGACTGAAAACTTATACATCTCACATAGCTTCTAGCTATTCCTGTTTCACAATCAAACTTTATATACTTTGATGCAGGTATTCGTTTGATTCTCTCTATGGTATCCCCAAAAACCAAGGTTACTTCACAGAAACATACCTTGACGTGAAAAAAGTATTAATTGATACCTTCTTTGGTCCTCCAAAAGAGGGAGTTTATAGCCCATCTGTGCAATACACTCTCTATGAAATGGCAAAGAATGTCCTCAGCAGGTCCTTCTCTCATTCCTCCCACTTGCACGTGAATGCACAATCACACTGAATTCCACAGATTTTTTAACTCTCTGTTTGTAGGTTTTAATTTCTCTGAAAATAACTTTTGGATTTTCAATATTTTAGGTTCCAAGTTATATCATCAGTGAAGCTGAAAATGCCAAATCTCCACTTCTTACCTGTCAACATATCAACAAAAGATAATCAGAGTATCGTTAAGGTAGGAATCTCCTTACAAAGGTTACCTTTTCCTTTTCGATGGTAATCTCTTTACAACTCTGCTTGTGGACTCTAATAAGTTTTGTTCGGTTCTTGCTATCTTAATTGTCAGTATTAGAGTGTTCTCAATTCAGTTCATGTGTTACTTTATTTCCTCAAAGAAGCATATTCTTGCATCATCTCTAGAGATAGTGAAAATAGTTCTATCAACTTTTGATTACATTCCATTCTTATTTTGCTCATGAATTTTCAAATTCTTCCATTTGCCTTGTTTTTGTTGTTTCACGGCCATCAGTTCCTCATTAGAATTAAGACTAGAACATAAAACTTCATCTTTAATCCACAACCCTTTTAATGTGTATAAATCTTGCTATTAGGTCGGTTGAAATTGCAGCCACCAGTTTCAATTATATATAGTGAACCTGTCATCTCAAACAATCTCATATGCTGACATTTTTTTAAAAAAGGAAACAAGTCTATTCATATAAATGTAAAATGAAAGAGATTAATGTTCCAAGGATAAAGCATATCTAGGATTAGCAGGCGCATCCGAACATCCGAACATCCGAACATCTCAACTAGGTTGACACCCCTTTATCGCCCTCATCATATCCTTATAACATAAAGTCAAAAATCAGCCAAGGCTTATAAATTCAGCCATTACATATAGGACAAACAGACTATTGATCTCATGTGTTAGACATATTGAAATAAAAAGGATGAAGAAGTACGAGATATACTAAGCTTAACCCAAGCCTTAGATCATCAACAATTTCCTCTTAACCCAAGCCTTAGATCATCAACAATTTCCTCTTCCCTCCCCTTTCTATTTATAACCGAGTTCGCTAACTTCTCAGCTAATTACCCTTGTGCCCTTACTAATGCCACAGATTTAGGATAAAGATATTGTTACTTTGAGTAGTCTTCTAACCTAAGAAGCACTACACGAACAGAGCCAACTCTGTACATCTCTAGTGTCTGGCAGATTTATTGTGCTAACAAGCATCAAATACTTAAACAACAAGTGAATTCCGTGCTTCTTAGCATTTCACGAGACATTGATATCGGTTTTTGTATTGTTAAAGAAAATGAAAATGGTGGTGGTGATGTTTTCGTTTTGCAGTTTGAGGATGATGTGTATCTGCCAACAGATGAACCGCATGGATCAATTGAAGCTTCCTTGAGCCGCTTCTCTTCAAAGCTATGA

mRNA sequence

ATGGCTCCCGACGACGCTGTAGTCGTAGTGAACGGCTTGAACTTTGAGCAGAGGCACGGCAAGGAACGAGTGCGAGTGGCTCGGGTTTGGCGGACCAAAGGCGGCCGTCATTTTATTGTTGAATGGAGCGTTGGCATCAGTCTTCTCTCCGATTGCGTCGCTGCATACGTTAGCGATGATAATTCCGATATTGTTGCAACCGATACTATGAAGAACACTGTATATGCAAAAGCCAAGGAGTGTTCTGACCAAACTTCAGTGGAAGATTTTGCAATTTTTCTTGCCAAGCACTTCACGTTATGTTACAGTCAGGTCACTACTACCATAGTGAAGATTGCAGAAAAGTCATGGGAGCGTGTATCTGTCAATGGTCAACCACACAACCATGGCTTCAAACTTGGATCTGAAAAGCACACAACAGAGGTGATATTTAAGAAGTCAGGTGTTTTACAAGTGAGCTCTGGTATTGAAGGACTATCTTTGCTGAAGACAACTCAATCAGGTTTTGAGCGATTTCATAGGGATAAGTACACAGCTCTTCCTGAGACACGAGAGAGGATATTAGCCACTGAAGTATCTGCTTCATGGAGGTATTCGTTTGATTCTCTCTATGGTATCCCCAAAAACCAAGGTTACTTCACAGAAACATACCTTGACGTGAAAAAAGTATTAATTGATACCTTCTTTGGTCCTCCAAAAGAGGGAGTTTATAGCCCATCTGTGCAATACACTCTCTATGAAATGGCAAAGAATGTCCTCAGCAGGTTCCAAGTTATATCATCAGTGAAGCTGAAAATGCCAAATCTCCACTTCTTACCTGTCAACATATCAACAAAAGATAATCAGAGTATCGTTAAGTTTGAGGATGATGTGTATCTGCCAACAGATGAACCGCATGGATCAATTGAAGCTTCCTTGAGCCGCTTCTCTTCAAAGCTATGA

Coding sequence (CDS)

ATGGCTCCCGACGACGCTGTAGTCGTAGTGAACGGCTTGAACTTTGAGCAGAGGCACGGCAAGGAACGAGTGCGAGTGGCTCGGGTTTGGCGGACCAAAGGCGGCCGTCATTTTATTGTTGAATGGAGCGTTGGCATCAGTCTTCTCTCCGATTGCGTCGCTGCATACGTTAGCGATGATAATTCCGATATTGTTGCAACCGATACTATGAAGAACACTGTATATGCAAAAGCCAAGGAGTGTTCTGACCAAACTTCAGTGGAAGATTTTGCAATTTTTCTTGCCAAGCACTTCACGTTATGTTACAGTCAGGTCACTACTACCATAGTGAAGATTGCAGAAAAGTCATGGGAGCGTGTATCTGTCAATGGTCAACCACACAACCATGGCTTCAAACTTGGATCTGAAAAGCACACAACAGAGGTGATATTTAAGAAGTCAGGTGTTTTACAAGTGAGCTCTGGTATTGAAGGACTATCTTTGCTGAAGACAACTCAATCAGGTTTTGAGCGATTTCATAGGGATAAGTACACAGCTCTTCCTGAGACACGAGAGAGGATATTAGCCACTGAAGTATCTGCTTCATGGAGGTATTCGTTTGATTCTCTCTATGGTATCCCCAAAAACCAAGGTTACTTCACAGAAACATACCTTGACGTGAAAAAAGTATTAATTGATACCTTCTTTGGTCCTCCAAAAGAGGGAGTTTATAGCCCATCTGTGCAATACACTCTCTATGAAATGGCAAAGAATGTCCTCAGCAGGTTCCAAGTTATATCATCAGTGAAGCTGAAAATGCCAAATCTCCACTTCTTACCTGTCAACATATCAACAAAAGATAATCAGAGTATCGTTAAGTTTGAGGATGATGTGTATCTGCCAACAGATGAACCGCATGGATCAATTGAAGCTTCCTTGAGCCGCTTCTCTTCAAAGCTATGA

Protein sequence

MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDDNSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERVSVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTALPETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPSVQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHGSIEASLSRFSSKL*

Homology

[Close]

BLAST of Chy10G184220 vs. ExPASy Swiss-Prot
Match: P53763 (Uricase-2 OS=Phaseolus vulgaris OX=3885 GN=URIII PE=1 SV=2)

HSP 1 Score: 459.9 bits (1182), Expect = 2.3e-128
Identity = 232/305 (76.07%), Postives = 254/305 (83.28%), Query Frame = 0


Query: 9   VVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDDNSDIVATD 68
           VV G  FEQRHGKERVRVARVWRT  GRHF+VEW VGI+L SDCV +Y+ DDNSDIVATD
Sbjct: 5   VVEGFKFEQRHGKERVRVARVWRTPQGRHFVVEWRVGITLFSDCVNSYLRDDNSDIVATD 64

Query: 69  TMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERVSVNGQPHN 128
           TMKNTVYAKAKECSD  SVEDFAI LAKHF   Y +VT  IV I EK WERV V+GQPH 
Sbjct: 65  TMKNTVYAKAKECSDILSVEDFAILLAKHFVSFYKKVTGAIVNIVEKPWERVIVDGQPHQ 124

Query: 129 HGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTALPETRERIL 188
           HGF LGSEKHTTE I +KSG LQ++SGIEGLS+LKTTQSGFE F R+KYTALP+TRERIL
Sbjct: 125 HGFTLGSEKHTTEAIVQKSGSLQLTSGIEGLSVLKTTQSGFENFIRNKYTALPDTRERIL 184

Query: 189 ATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPSVQYTLYEM 248
           ATEV+A WRYS++SLY +P+   YFT+ YL+VKKVL DTFFGPP  GVYSPSVQ TLY M
Sbjct: 185 ATEVTALWRYSYESLYNLPQKPLYFTDKYLEVKKVLADTFFGPPNRGVYSPSVQNTLYLM 244

Query: 249 AKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHGSIEASLSR 308
           AK  L+RF  I+ V LKMPNLHFLPVNIS+KD   IVKFEDDVYLPTDEPHGSIEASLSR
Sbjct: 245 AKATLNRFPDIAYVHLKMPNLHFLPVNISSKDG-PIVKFEDDVYLPTDEPHGSIEASLSR 304

Query: 309 FSSKL 314
             SKL
Sbjct: 305 VWSKL 308

[Close]

BLAST of Chy10G184220 vs. ExPASy Swiss-Prot
Match: O04104 (Uricase-2 isozyme 2 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.1e-126
Identity = 228/305 (74.75%), Postives = 251/305 (82.30%), Query Frame = 0


Query: 9   VVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDDNSDIVATD 68
           VV G  FEQRHGKERVRVARVW+T+ G+HF+VEW VGI+L SDCV +Y+ DDNSDIVATD
Sbjct: 6   VVEGFKFEQRHGKERVRVARVWKTRQGQHFVVEWRVGITLFSDCVNSYLRDDNSDIVATD 65

Query: 69  TMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERVSVNGQPHN 128
           TMKNTVYAKAKECSD  S EDFAI LAKHF   Y +VT  IV I EK WERV V+GQPH 
Sbjct: 66  TMKNTVYAKAKECSDILSAEDFAILLAKHFVSFYKKVTGAIVNIVEKPWERVIVDGQPHE 125

Query: 129 HGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTALPETRERIL 188
           HGFKLGSEKHTTE I +KSG LQ++SGIEGLS+LKTTQSGF  F RDKYTALP+TRERIL
Sbjct: 126 HGFKLGSEKHTTEAIVQKSGSLQLTSGIEGLSVLKTTQSGFVNFIRDKYTALPDTRERIL 185

Query: 189 ATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPSVQYTLYEM 248
           ATEV+A WRYS++S Y +P+   YFTE Y +VKKVL DTFFGPP  GVYSPSVQ TLY M
Sbjct: 186 ATEVTALWRYSYESQYSLPQKPLYFTEKYQEVKKVLADTFFGPPNGGVYSPSVQNTLYLM 245

Query: 249 AKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHGSIEASLSR 308
           AK  L+RF  I+ V LKMPNLHFLPVNIS KD   IVKFEDDVYLPTDEPHGSI+ASLSR
Sbjct: 246 AKATLNRFPDIAYVSLKMPNLHFLPVNISNKDG-PIVKFEDDVYLPTDEPHGSIQASLSR 305

Query: 309 FSSKL 314
             SKL
Sbjct: 306 LWSKL 309

[Close]

BLAST of Chy10G184220 vs. ExPASy Swiss-Prot
Match: P04670 (Uricase-2 isozyme 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 451.8 bits (1161), Expect = 6.2e-126
Identity = 225/305 (73.77%), Postives = 254/305 (83.28%), Query Frame = 0


Query: 9   VVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDDNSDIVATD 68
           VV G  FEQRHGKERVRVARVW+T+ G+HFIVEW VGI+L SDCV +Y+ DDNSDIVATD
Sbjct: 6   VVEGFKFEQRHGKERVRVARVWKTRQGQHFIVEWRVGITLFSDCVNSYLRDDNSDIVATD 65

Query: 69  TMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERVSVNGQPHN 128
           TMKNTVYAKAKECSD  S E+FAI LAKHF   Y +VT  IV I EK WERV+V+GQPH 
Sbjct: 66  TMKNTVYAKAKECSDILSAEEFAILLAKHFVSFYQKVTGAIVNIVEKPWERVTVDGQPHE 125

Query: 129 HGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTALPETRERIL 188
           HGFKLGSEKHTTE I +KSG LQ++SGIEGLS+LKTTQSGF  F RDKYTALP+TRER++
Sbjct: 126 HGFKLGSEKHTTEAIVQKSGSLQLTSGIEGLSVLKTTQSGFVNFIRDKYTALPDTRERMV 185

Query: 189 ATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPSVQYTLYEM 248
           ATEV+A WRYS++SLY +P+   YFTE Y +VKKVL DTFFGPPK GVYSPSVQ TLY M
Sbjct: 186 ATEVTALWRYSYESLYSLPQKPLYFTEKYQEVKKVLADTFFGPPKGGVYSPSVQNTLYLM 245

Query: 249 AKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHGSIEASLSR 308
           AK  L+RF  I+ V LK+PNLHF+PVNIS +D   IVKFEDDVYLPTDEPHGSI+ASLSR
Sbjct: 246 AKATLNRFPDIAYVSLKLPNLHFIPVNISNQDG-PIVKFEDDVYLPTDEPHGSIQASLSR 305

Query: 309 FSSKL 314
             SKL
Sbjct: 306 LWSKL 309

[Close]

BLAST of Chy10G184220 vs. ExPASy Swiss-Prot
Match: P34798 (Uricase-2 isozyme 1 OS=Canavalia lineata OX=28957 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.4e-125
Identity = 226/305 (74.10%), Postives = 252/305 (82.62%), Query Frame = 0


Query: 9   VVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDDNSDIVATD 68
           +V G  F+QRHGKERV+VARVW+TK G +FIVEW VG SLLSDCV +YV DDNSDIVATD
Sbjct: 5   IVGGFKFDQRHGKERVQVARVWKTKQGWYFIVEWRVGNSLLSDCVNSYVRDDNSDIVATD 64

Query: 69  TMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERVSVNGQPHN 128
           TMKNTVYAKAKECS+  SVEDFAI LAKHF   Y QVT  IV I EK WERVSV+GQPH 
Sbjct: 65  TMKNTVYAKAKECSEILSVEDFAILLAKHFISFYKQVTAAIVNIVEKPWERVSVDGQPHE 124

Query: 129 HGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTALPETRERIL 188
           HGFKLGSE+HT E I +KSG LQ++SGIEGLSLLKTT+SGFE F RDKYTALPET ER+L
Sbjct: 125 HGFKLGSERHTAEAIVQKSGALQLTSGIEGLSLLKTTKSGFEGFIRDKYTALPETHERML 184

Query: 189 ATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPSVQYTLYEM 248
           ATEV+A WRYS++SLY IP+   YFT+ YL+VKKVL D FFGPP  GVYSPSVQ TLY M
Sbjct: 185 ATEVTALWRYSYESLYSIPQKPLYFTDKYLEVKKVLADNFFGPPNVGVYSPSVQNTLYLM 244

Query: 249 AKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHGSIEASLSR 308
           AK  L+RF  I+S++LKMPN+HFLPVNIS KD   IVKFE DVYLPTDEPHGSI+ASL R
Sbjct: 245 AKAALNRFPEIASIQLKMPNIHFLPVNISNKDG-PIVKFEADVYLPTDEPHGSIQASLRR 304

Query: 309 FSSKL 314
             SKL
Sbjct: 305 LWSKL 308

[Close]

BLAST of Chy10G184220 vs. ExPASy Swiss-Prot
Match: P34799 (Uricase-2 isozyme 2 OS=Canavalia lineata OX=28957 PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.4e-124
Identity = 226/305 (74.10%), Postives = 250/305 (81.97%), Query Frame = 0


Query: 9   VVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDDNSDIVATD 68
           +V G  F+QRHGKERVRVARVW+TK G +FIVEW VGISLLSDCV +YV DDNSDIVATD
Sbjct: 5   IVGGFKFDQRHGKERVRVARVWKTKKGGYFIVEWRVGISLLSDCVNSYVRDDNSDIVATD 64

Query: 69  TMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERVSVNGQPHN 128
           TMKNTVYAKAKECS+  SVEDFAI LAKHF   Y QVT  IV I EK WERVSV+GQPH 
Sbjct: 65  TMKNTVYAKAKECSEILSVEDFAILLAKHFISFYKQVTAAIVNIVEKPWERVSVDGQPHE 124

Query: 129 HGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTALPETRERIL 188
           HGFKLGSE+HT E I +KSG LQ++SGIEGLSLLKTT+SGFE F RDKYTALPET ER+L
Sbjct: 125 HGFKLGSERHTAEAIVQKSGALQLTSGIEGLSLLKTTKSGFEGFIRDKYTALPETHERML 184

Query: 189 ATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPSVQYTLYEM 248
           ATEV+A WRYS++SLY IP+   YFT+ YL+VKKVL DTFFGPP  GVYSPSVQ TLY M
Sbjct: 185 ATEVTALWRYSYESLYSIPQKPLYFTDKYLEVKKVLADTFFGPPNVGVYSPSVQNTLYLM 244

Query: 249 AKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHGSIEASLSR 308
           AK         SS++LKMPN+HFLPVNIS KD   IVKF+DDVY PTDEPHGSI+ASLSR
Sbjct: 245 AK-------AHSSIQLKMPNIHFLPVNISNKDG-PIVKFDDDVYFPTDEPHGSIQASLSR 301

Query: 309 FSSKL 314
             SKL
Sbjct: 305 LWSKL 301

[Close]

BLAST of Chy10G184220 vs. ExPASy TrEMBL
Match: A0A0A0KRD9 (Uricase OS=Cucumis sativus OX=3659 GN=Csa_5G613510 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.0e-172
Identity = 306/313 (97.76%), Postives = 309/313 (98.72%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAP+DAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD
Sbjct: 39  MAPEDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 98

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTL Y+QVTTTIVKIAEKSWERV
Sbjct: 99  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLYYNQVTTTIVKIAEKSWERV 158

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIE LSLLKTTQSGFERFHRDKYTAL
Sbjct: 159 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEELSLLKTTQSGFERFHRDKYTAL 218

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATEVSA WRYSFDSLY IPKNQGYFTETYLDVKKVL+DTFFGPPKEGVYSPS
Sbjct: 219 PETRERILATEVSALWRYSFDSLYDIPKNQGYFTETYLDVKKVLVDTFFGPPKEGVYSPS 278

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG
Sbjct: 279 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 338

Query: 301 SIEASLSRFSSKL 314
           SIEASLSRFSSKL
Sbjct: 339 SIEASLSRFSSKL 351

[Close]

BLAST of Chy10G184220 vs. ExPASy TrEMBL
Match: A0A5D3CBH0 (Uricase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001860 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 301/313 (96.17%), Postives = 306/313 (97.76%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAPDDAVVVVNGLNFEQRHGKERVRVARVWR KGGRHFIVEWSVGISLLSDC+AAYVSDD
Sbjct: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRAKGGRHFIVEWSVGISLLSDCIAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQTSVEDFAI LAKHFT  Y+QVTT IVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAILLAKHFTSYYNQVTTAIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGS KHTTEVI KKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL
Sbjct: 121 SVNGQPHNHGFKLGSGKHTTEVISKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATEVSASWRYSF+SLYGIPKNQGYFTETYLDVKKVL+DTFFGPPKEGVYSPS
Sbjct: 181 PETRERILATEVSASWRYSFESLYGIPKNQGYFTETYLDVKKVLVDTFFGPPKEGVYSPS 240

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG
Sbjct: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300

Query: 301 SIEASLSRFSSKL 314
           SIEASLSRF+SKL
Sbjct: 301 SIEASLSRFTSKL 313

[Close]

BLAST of Chy10G184220 vs. ExPASy TrEMBL
Match: A0A1S3BGL8 (Uricase OS=Cucumis melo OX=3656 GN=LOC103489372 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 301/313 (96.17%), Postives = 306/313 (97.76%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAPDDAVVVVNGLNFEQRHGKERVRVARVWR KGGRHFIVEWSVGISLLSDC+AAYVSDD
Sbjct: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRAKGGRHFIVEWSVGISLLSDCIAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQTSVEDFAI LAKHFT  Y+QVTT IVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAILLAKHFTSYYNQVTTAIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGS KHTTEVI KKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL
Sbjct: 121 SVNGQPHNHGFKLGSGKHTTEVISKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATEVSASWRYSF+SLYGIPKNQGYFTETYLDVKKVL+DTFFGPPKEGVYSPS
Sbjct: 181 PETRERILATEVSASWRYSFESLYGIPKNQGYFTETYLDVKKVLVDTFFGPPKEGVYSPS 240

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG
Sbjct: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300

Query: 301 SIEASLSRFSSKL 314
           SIEASLSRF+SKL
Sbjct: 301 SIEASLSRFTSKL 313

[Close]

BLAST of Chy10G184220 vs. ExPASy TrEMBL
Match: A0A5A7SZ15 (Uricase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006600 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.1e-168
Identity = 301/314 (95.86%), Postives = 306/314 (97.45%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAPDDAVVVVNGLNFEQRHGKERVRVARVWR KGGRHFIVEWSVGISLLSDC+AAYVSDD
Sbjct: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRAKGGRHFIVEWSVGISLLSDCIAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQTSVEDFAI LAKHFT  Y+QVTT IVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAILLAKHFTSYYNQVTTAIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTT-QSGFERFHRDKYTA 180
           SVNGQPHNHGFKLGS KHTTEVI KKSGVLQVSSGIEGLSLLKTT QSGFERFHRDKYTA
Sbjct: 121 SVNGQPHNHGFKLGSGKHTTEVISKKSGVLQVSSGIEGLSLLKTTQQSGFERFHRDKYTA 180

Query: 181 LPETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSP 240
           LPETRERILATEVSASWRYSF+SLYGIPKNQGYFTETYLDVKKVL+DTFFGPPKEGVYSP
Sbjct: 181 LPETRERILATEVSASWRYSFESLYGIPKNQGYFTETYLDVKKVLVDTFFGPPKEGVYSP 240

Query: 241 SVQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPH 300
           SVQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPH
Sbjct: 241 SVQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPH 300

Query: 301 GSIEASLSRFSSKL 314
           GSIEASLSRF+SKL
Sbjct: 301 GSIEASLSRFTSKL 314

[Close]

BLAST of Chy10G184220 vs. ExPASy TrEMBL
Match: A0A6J1J8L0 (Uricase OS=Cucurbita maxima OX=3661 GN=LOC111482298 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 5.2e-160
Identity = 287/313 (91.69%), Postives = 294/313 (93.93%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MA DD VVVVNGLNFEQRHGKERVRVARVWR K GR F VEW+VGISLLSDCVAAYVSDD
Sbjct: 1   MALDDDVVVVNGLNFEQRHGKERVRVARVWRNKDGRQFFVEWNVGISLLSDCVAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQ SVEDFAI LAKHFTL Y QVTTTIVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQLSVEDFAILLAKHFTLSYKQVTTTIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL
Sbjct: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATE+SASWRYSFDSLY IPK Q YFTETYLDVKK+L DTFFGPPKEGVYSPS
Sbjct: 181 PETRERILATEMSASWRYSFDSLYSIPKKQFYFTETYLDVKKLLADTFFGPPKEGVYSPS 240

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTL+EMAKNVLSRF VISSV+LKMPNLHFLP NISTKDN+SIVKFE DVY+PTDEPHG
Sbjct: 241 VQYTLHEMAKNVLSRFHVISSVRLKMPNLHFLPANISTKDNRSIVKFEHDVYVPTDEPHG 300

Query: 301 SIEASLSRFSSKL 314
           SIEA LSR SSKL
Sbjct: 301 SIEACLSRLSSKL 313

[Close]

BLAST of Chy10G184220 vs. NCBI nr
Match: XP_004135043.2 (uricase-2 [Cucumis sativus] >KGN52173.1 hypothetical protein Csa_008321 [Cucumis sativus])

HSP 1 Score: 613 bits (1581), Expect = 1.67e-220
Identity = 306/313 (97.76%), Postives = 309/313 (98.72%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAP+DAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD
Sbjct: 39  MAPEDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 98

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTL Y+QVTTTIVKIAEKSWERV
Sbjct: 99  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLYYNQVTTTIVKIAEKSWERV 158

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIE LSLLKTTQSGFERFHRDKYTAL
Sbjct: 159 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEELSLLKTTQSGFERFHRDKYTAL 218

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATEVSA WRYSFDSLY IPKNQGYFTETYLDVKKVL+DTFFGPPKEGVYSPS
Sbjct: 219 PETRERILATEVSALWRYSFDSLYDIPKNQGYFTETYLDVKKVLVDTFFGPPKEGVYSPS 278

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG
Sbjct: 279 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 338

Query: 301 SIEASLSRFSSKL 313
           SIEASLSRFSSKL
Sbjct: 339 SIEASLSRFSSKL 351

[Close]

BLAST of Chy10G184220 vs. NCBI nr
Match: XP_008446742.1 (PREDICTED: uricase-2 [Cucumis melo] >TYK09193.1 uricase-2 [Cucumis melo var. makuwa])

HSP 1 Score: 605 bits (1561), Expect = 4.55e-218
Identity = 301/313 (96.17%), Postives = 306/313 (97.76%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAPDDAVVVVNGLNFEQRHGKERVRVARVWR KGGRHFIVEWSVGISLLSDC+AAYVSDD
Sbjct: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRAKGGRHFIVEWSVGISLLSDCIAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQTSVEDFAI LAKHFT  Y+QVTT IVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAILLAKHFTSYYNQVTTAIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGS KHTTEVI KKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL
Sbjct: 121 SVNGQPHNHGFKLGSGKHTTEVISKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATEVSASWRYSF+SLYGIPKNQGYFTETYLDVKKVL+DTFFGPPKEGVYSPS
Sbjct: 181 PETRERILATEVSASWRYSFESLYGIPKNQGYFTETYLDVKKVLVDTFFGPPKEGVYSPS 240

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG
Sbjct: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300

Query: 301 SIEASLSRFSSKL 313
           SIEASLSRF+SKL
Sbjct: 301 SIEASLSRFTSKL 313

[Close]

BLAST of Chy10G184220 vs. NCBI nr
Match: KAA0034641.1 (uricase-2 [Cucumis melo var. makuwa])

HSP 1 Score: 601 bits (1549), Expect = 3.18e-216
Identity = 301/314 (95.86%), Postives = 306/314 (97.45%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAPDDAVVVVNGLNFEQRHGKERVRVARVWR KGGRHFIVEWSVGISLLSDC+AAYVSDD
Sbjct: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRAKGGRHFIVEWSVGISLLSDCIAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQTSVEDFAI LAKHFT  Y+QVTT IVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAILLAKHFTSYYNQVTTAIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQ-SGFERFHRDKYTA 180
           SVNGQPHNHGFKLGS KHTTEVI KKSGVLQVSSGIEGLSLLKTTQ SGFERFHRDKYTA
Sbjct: 121 SVNGQPHNHGFKLGSGKHTTEVISKKSGVLQVSSGIEGLSLLKTTQQSGFERFHRDKYTA 180

Query: 181 LPETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSP 240
           LPETRERILATEVSASWRYSF+SLYGIPKNQGYFTETYLDVKKVL+DTFFGPPKEGVYSP
Sbjct: 181 LPETRERILATEVSASWRYSFESLYGIPKNQGYFTETYLDVKKVLVDTFFGPPKEGVYSP 240

Query: 241 SVQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPH 300
           SVQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPH
Sbjct: 241 SVQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPH 300

Query: 301 GSIEASLSRFSSKL 313
           GSIEASLSRF+SKL
Sbjct: 301 GSIEASLSRFTSKL 314

[Close]

BLAST of Chy10G184220 vs. NCBI nr
Match: XP_038892092.1 (uricase-2 isozyme 2 [Benincasa hispida])

HSP 1 Score: 599 bits (1545), Expect = 1.25e-215
Identity = 299/313 (95.53%), Postives = 301/313 (96.17%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTK GRHFIVEWSVGISLLSDCVAAYVSDD
Sbjct: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKDGRHFIVEWSVGISLLSDCVAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQ SVEDFAI LAKHFTL Y QVTT IVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQISVEDFAILLAKHFTLYYKQVTTAIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGSEKHT E IFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL
Sbjct: 121 SVNGQPHNHGFKLGSEKHTAEAIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATEVSASWRYSFDSLYGIPK Q YF ETYLDVKKVL+DTFFGPPKEGVYSPS
Sbjct: 181 PETRERILATEVSASWRYSFDSLYGIPKKQCYFAETYLDVKKVLVDTFFGPPKEGVYSPS 240

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTLYEM KNVLSRFQVISSV+LKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG
Sbjct: 241 VQYTLYEMGKNVLSRFQVISSVRLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300

Query: 301 SIEASLSRFSSKL 313
           SIEASLSRFSSKL
Sbjct: 301 SIEASLSRFSSKL 313

[Close]

BLAST of Chy10G184220 vs. NCBI nr
Match: XP_022983819.1 (uricase-2 isozyme 2-like [Cucurbita maxima])

HSP 1 Score: 573 bits (1478), Expect = 2.03e-205
Identity = 287/313 (91.69%), Postives = 294/313 (93.93%), Query Frame = 0


Query: 1   MAPDDAVVVVNGLNFEQRHGKERVRVARVWRTKGGRHFIVEWSVGISLLSDCVAAYVSDD 60
           MA DD VVVVNGLNFEQRHGKERVRVARVWR K GR F VEW+VGISLLSDCVAAYVSDD
Sbjct: 1   MALDDDVVVVNGLNFEQRHGKERVRVARVWRNKDGRQFFVEWNVGISLLSDCVAAYVSDD 60

Query: 61  NSDIVATDTMKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERV 120
           NSDIVATDTMKNTVYAKAKECSDQ SVEDFAI LAKHFTL Y QVTTTIVKIAEKSWERV
Sbjct: 61  NSDIVATDTMKNTVYAKAKECSDQLSVEDFAILLAKHFTLSYKQVTTTIVKIAEKSWERV 120

Query: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180
           SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL
Sbjct: 121 SVNGQPHNHGFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTAL 180

Query: 181 PETRERILATEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPS 240
           PETRERILATE+SASWRYSFDSLY IPK Q YFTETYLDVKK+L DTFFGPPKEGVYSPS
Sbjct: 181 PETRERILATEMSASWRYSFDSLYSIPKKQFYFTETYLDVKKLLADTFFGPPKEGVYSPS 240

Query: 241 VQYTLYEMAKNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHG 300
           VQYTL+EMAKNVLSRF VISSV+LKMPNLHFLP NISTKDN+SIVKFE DVY+PTDEPHG
Sbjct: 241 VQYTLHEMAKNVLSRFHVISSVRLKMPNLHFLPANISTKDNRSIVKFEHDVYVPTDEPHG 300

Query: 301 SIEASLSRFSSKL 313
           SIEA LSR SSKL
Sbjct: 301 SIEACLSRLSSKL 313

[Close]

BLAST of Chy10G184220 vs. TAIR 10
Match: AT2G26230.1 (uricase / urate oxidase / nodulin 35, putative )

HSP 1 Score: 425.6 bits (1093), Expect = 3.4e-119
Identity = 209/304 (68.75%), Postives = 246/304 (80.92%), Query Frame = 0


Query: 11  NGLNFEQRHGKERVRVARVWR-TKGGRHFIVEWSVGISLLSDCVAAYVSDDNSDIVATDT 70
           +G+  +QRHGK RVRV RVWR    G H  VEW+V ISLLS C+++Y  DDNSDIVATDT
Sbjct: 6   DGIRLDQRHGKARVRVGRVWRHAHDGSHHFVEWNVSISLLSHCLSSYRLDDNSDIVATDT 65

Query: 71  MKNTVYAKAKECSDQTSVEDFAIFLAKHFTLCYSQVTTTIVKIAEKSWERVSVNGQPHNH 130
           +KNTVY KAKEC D+ SVE+FAI + KHF   Y QV T IV I EK WERVS++G+PH H
Sbjct: 66  IKNTVYVKAKECGDRLSVEEFAILIGKHFCSFYPQVFTAIVNIIEKPWERVSIDGKPHLH 125

Query: 131 GFKLGSEKHTTEVIFKKSGVLQVSSGIEGLSLLKTTQSGFERFHRDKYTALPETRERILA 190
           GFKLGSE HTTE   +KSG L ++SGI GL+LLKTTQSGFERF RDKYT LPETRER+LA
Sbjct: 126 GFKLGSENHTTEARVEKSGALNLTSGIGGLALLKTTQSGFERFVRDKYTILPETRERMLA 185

Query: 191 TEVSASWRYSFDSLYGIPKNQGYFTETYLDVKKVLIDTFFGPPKEGVYSPSVQYTLYEMA 250
           TEV+ASWRYS++S+  IP    YF+E ++DVKKVL+DTFFGPP+ GVYSPSVQ TLY M 
Sbjct: 186 TEVNASWRYSYESVASIPTKGLYFSEKFMDVKKVLMDTFFGPPETGVYSPSVQRTLYLMG 245

Query: 251 KNVLSRFQVISSVKLKMPNLHFLPVNISTKDNQSIVKFEDDVYLPTDEPHGSIEASLSRF 310
             VL RF  +SS+ LKMPN+HFLPVN+STK+N S+VKF+DDVYLPTDEPHGSIEA+LSR 
Sbjct: 246 SAVLKRFADVSSIHLKMPNIHFLPVNLSTKENPSMVKFKDDVYLPTDEPHGSIEATLSRI 305

Query: 311 SSKL 314
           +SKL
Sbjct: 306 TSKL 309

The following BLAST results are available for this feature:

BLAST of Chy10G184220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P53763	2.3e-128	76.07	Uricase-2 OS=Phaseolus vulgaris OX=3885 GN=URIII PE=1 SV=2	[more]
O04104	2.1e-126	74.75	Uricase-2 isozyme 2 OS=Glycine max OX=3847 PE=1 SV=1	[more]
P04670	6.2e-126	73.77	Uricase-2 isozyme 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
P34798	2.4e-125	74.10	Uricase-2 isozyme 1 OS=Canavalia lineata OX=28957 PE=2 SV=1	[more]
P34799	3.4e-124	74.10	Uricase-2 isozyme 2 OS=Canavalia lineata OX=28957 PE=2 SV=1	[more]

BLAST of Chy10G184220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KRD9	6.0e-172	97.76	Uricase OS=Cucumis sativus OX=3659 GN=Csa_5G613510 PE=3 SV=1	[more]
A0A5D3CBH0	1.2e-169	96.17	Uricase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001860 PE=3 ...	[more]
A0A1S3BGL8	1.2e-169	96.17	Uricase OS=Cucumis melo OX=3656 GN=LOC103489372 PE=3 SV=1	[more]
A0A5A7SZ15	3.1e-168	95.86	Uricase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006600 PE=3 S...	[more]
A0A6J1J8L0	5.2e-160	91.69	Uricase OS=Cucurbita maxima OX=3661 GN=LOC111482298 PE=3 SV=1	[more]

BLAST of Chy10G184220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135043.2	1.67e-220	97.76	uricase-2 [Cucumis sativus] >KGN52173.1 hypothetical protein Csa_008321 [Cucumis...	[more]
XP_008446742.1	4.55e-218	96.17	PREDICTED: uricase-2 [Cucumis melo] >TYK09193.1 uricase-2 [Cucumis melo var. mak...	[more]
KAA0034641.1	3.18e-216	95.86	uricase-2 [Cucumis melo var. makuwa]	[more]
XP_038892092.1	1.25e-215	95.53	uricase-2 isozyme 2 [Benincasa hispida]	[more]
XP_022983819.1	2.03e-205	91.69	uricase-2 isozyme 2-like [Cucurbita maxima]	[more]

BLAST of Chy10G184220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26230.1	3.4e-119	68.75	uricase / urate oxidase / nodulin 35, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002042	Uricase	PRINTS	PR00093	URICASE	coord: 60..74 score: 87.3 coord: 97..110 score: 42.86 coord: 20..31 score: 65.48 coord: 159..175 score: 70.03 coord: 175..187 score: 64.47
IPR002042	Uricase	PIRSF	PIRSF000241	Urate_oxidase	coord: 5..313 e-value: 2.3E-99 score: 330.2
IPR002042	Uricase	TIGRFAM	TIGR03383	TIGR03383	coord: 17..308 e-value: 1.1E-99 score: 331.4
IPR002042	Uricase	PFAM	PF01014	Uricase	coord: 18..142 e-value: 7.3E-30 score: 104.0 coord: 153..303 e-value: 1.4E-25 score: 90.1
IPR002042	Uricase	PANTHER	PTHR42874	URICASE	coord: 11..313
None	No IPR available	GENE3D	3.10.270.10	Urate Oxidase;	coord: 13..313 e-value: 8.9E-104 score: 348.6
None	No IPR available	PIRSR	PIRSR000241-1	PIRSR000241-1	coord: 17..313 e-value: 3.1E-122 score: 405.1
None	No IPR available	PIRSR	PIRSR000241-2	PIRSR000241-2	coord: 17..313 e-value: 3.1E-122 score: 405.1
None	No IPR available	CDD	cd00445	Uricase	coord: 18..308 e-value: 1.65221E-145 score: 409.062
None	No IPR available	SUPERFAMILY	55620	Tetrahydrobiopterin biosynthesis enzymes-like	coord: 15..137
None	No IPR available	SUPERFAMILY	55620	Tetrahydrobiopterin biosynthesis enzymes-like	coord: 148..308
IPR019842	Uricase, conserved site	PROSITE	PS00366	URICASE	coord: 159..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G184220.1	Chy10G184220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0019628	urate catabolic process
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0004846	urate oxidase activity