Homology
BLAST of Chy10G184120 vs. ExPASy Swiss-Prot
Match:
Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)
HSP 1 Score: 93.2 bits (230), Expect = 4.8e-18
Identity = 43/107 (40.19%), Postives = 65/107 (60.75%), Query Frame = 0
Query: 160 IGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFI 219
IG G +L G++ N + ++L RK GDT YKIP+GGLF +V +Y EI+ + G+A
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216
Query: 220 SQELYPLCFSIGTAVYLAGRSYATRKWYISKFETFPNNVKALLPFVF 267
S + F+ T +L+GR+ +WY+ KFE +P K L+PF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263
BLAST of Chy10G184120 vs. ExPASy Swiss-Prot
Match:
P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)
HSP 1 Score: 91.7 bits (226), Expect = 1.4e-17
Identity = 42/107 (39.25%), Postives = 65/107 (60.75%), Query Frame = 0
Query: 160 IGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFI 219
IG G +L G++ N + ++L RK GDT YKIP+GGLF +V +Y EI+ + G+A
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212
Query: 220 SQELYPLCFSIGTAVYLAGRSYATRKWYISKFETFPNNVKALLPFVF 267
S + F+ T +L+GR+ +WY+ KFE +P K ++PF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259
BLAST of Chy10G184120 vs. ExPASy Swiss-Prot
Match:
Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)
HSP 1 Score: 90.1 bits (222), Expect = 4.0e-17
Identity = 55/185 (29.73%), Postives = 96/185 (51.89%), Query Frame = 0
Query: 85 LFPSGDERVFLLKSAL---TLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMI 144
++ + + F K AL +LH+ KRI+E +F+H++S + + + SY+ M+
Sbjct: 115 IYGTDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMV 174
Query: 145 YTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFV 204
E PI+ +G+G ++IG + N+ H L R G T KIP+G LF FV
Sbjct: 175 SYFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAGSTERKIPRGLLFEFV 234
Query: 205 ICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRSYATRKWYISKF-ETFPNNVKA 264
CP+Y EI+ + GF+ ++Q L F++ A + + + Y +F + +P + K
Sbjct: 235 SCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDKYPKSRKI 294
Query: 265 LLPFV 266
L+PF+
Sbjct: 295 LIPFL 299
BLAST of Chy10G184120 vs. ExPASy Swiss-Prot
Match:
Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)
HSP 1 Score: 89.4 bits (220), Expect = 6.9e-17
Identity = 63/200 (31.50%), Postives = 104/200 (52.00%), Query Frame = 0
Query: 82 SFWLFPSG----DERVFLLKSALTLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMST 141
+F+LFPSG + + FLL S H+F R VL+ + + +S++ FM+
Sbjct: 62 TFFLFPSGQHFYNPKSFLLISPFLFHYFNR--TVLYPLRLARNTTQTRGFPVSVA-FMAF 121
Query: 142 AIMIYTQHL-SQGLPEPPIDLKN---------IGVGSFLIGIIGNFYHHYLLSQTRKQGD 201
+ +L ++ + D +N G+ F++G+ N +L +KQGD
Sbjct: 122 GFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGD 181
Query: 202 TAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRSYATRKWY 261
YKIP+GGLF V CP+Y EI+ +FG+A ++ F + T L R+ ATR WY
Sbjct: 182 GGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWY 241
Query: 262 ISKF-ETFPNNVKALLPFVF 267
+ KF + +P + KA++PF++
Sbjct: 242 LEKFKDDYPKDRKAVIPFIY 258
BLAST of Chy10G184120 vs. ExPASy Swiss-Prot
Match:
O18765 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=2)
HSP 1 Score: 88.2 bits (217), Expect = 1.5e-16
Identity = 41/108 (37.96%), Postives = 69/108 (63.89%), Query Frame = 0
Query: 159 NIGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAF 218
++GV F++G+ N + Y+L Q RK G+ YKIP+GGLF++V ++L EII + G+A
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206
Query: 219 ISQELYPLCFSIGTAVYLAGRSYATRKWYISKFETFPNNVKALLPFVF 267
+ L L F+ + +L R++ ++Y+ FE +P + KAL+PF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254
BLAST of Chy10G184120 vs. ExPASy TrEMBL
Match:
A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)
HSP 1 Score: 500.4 bits (1287), Expect = 4.8e-138
Identity = 248/266 (93.23%), Postives = 256/266 (96.24%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
MD+LSAFLFPPPPSTFVNVMTVVS+T+LAMAGLSEI GKHLQYSKFWNAN GSKLSA+E
Sbjct: 11 MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
KLSSKYGMLL YTPAFLAGAASFWLFPSGDER FLLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 71 KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MVLKTAIQIS SYF+STAIMIYTQHLSQGLPEPPIDLKNIGVG FL GIIGNFYHHYLLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
QTRKQ +TAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTA+YLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 267
YATRKWYISKFE FPN+VKALLPFVF
Sbjct: 251 YATRKWYISKFENFPNHVKALLPFVF 276
BLAST of Chy10G184120 vs. ExPASy TrEMBL
Match:
A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)
HSP 1 Score: 478.8 bits (1231), Expect = 1.5e-131
Identity = 243/266 (91.35%), Postives = 245/266 (92.11%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANG SKLSAEE
Sbjct: 21 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
K+SSKYGMLLAYTPAFLAGAASFWLFPSGDER VLFIHKYSSK
Sbjct: 81 KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------------VLFIHKYSSK 140
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
QTRKQGDTAYKIPKGGLFSFV+CPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 267
YATRKWYISKFE FPNNVKALLPFVF
Sbjct: 261 YATRKWYISKFEAFPNNVKALLPFVF 268
BLAST of Chy10G184120 vs. ExPASy TrEMBL
Match:
A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)
HSP 1 Score: 456.1 bits (1172), Expect = 1.0e-124
Identity = 222/266 (83.46%), Postives = 242/266 (90.98%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN K +EE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
KLSSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MV TAI+IS SY +ST IMIYTQHLSQGLPEPPIDLKNIG+ FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
+TRK G+T YKIPKGGLFS VICPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 267
YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of Chy10G184120 vs. ExPASy TrEMBL
Match:
A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)
HSP 1 Score: 455.3 bits (1170), Expect = 1.8e-124
Identity = 221/266 (83.08%), Postives = 242/266 (90.98%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN K +EE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
KLSSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MV TAI+IS SY +ST IMIYTQHLSQG+PEPPIDLKNIG+ FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
+TRK G+T YKIPKGGLFS VICPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 267
YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of Chy10G184120 vs. ExPASy TrEMBL
Match:
A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)
HSP 1 Score: 446.8 bits (1148), Expect = 6.3e-122
Identity = 216/266 (81.20%), Postives = 240/266 (90.23%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
MD+ SAFLFPPPPSTFVNVMTVVS+TA+AM+G SE+NGKHLQYSKFWNANG SK S+EE
Sbjct: 1 MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
K+SSKYGMLLAYTPAFLAGAASFW FPS D R+ LLKSALT HFFKRIFEVLF+HKYSSK
Sbjct: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MVL TA +ISLSYF+STAIMIYTQHLSQG P P IDLKNIG+G F++GIIGNFYHHYLLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
+TR +G+ YKIP GGLF VICPHYLFEII YFGFAFISQ ++PLCF+ GTA+YLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 267
YATRKWY+SKFE FPN+VKALLPF+F
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266
BLAST of Chy10G184120 vs. NCBI nr
Match:
XP_004135052.2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothetical protein Csa_008329 [Cucumis sativus])
HSP 1 Score: 521 bits (1342), Expect = 6.03e-186
Identity = 261/266 (98.12%), Postives = 263/266 (98.87%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANG SKLSAEE
Sbjct: 21 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
K+SSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 81 KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
QTRKQGDTAYKIPKGGLFSFV+CPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 266
YATRKWYISKFE FPNNVKALLPFVF
Sbjct: 261 YATRKWYISKFEAFPNNVKALLPFVF 286
BLAST of Chy10G184120 vs. NCBI nr
Match:
XP_008446722.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo])
HSP 1 Score: 499 bits (1284), Expect = 2.89e-177
Identity = 248/266 (93.23%), Postives = 256/266 (96.24%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
MD+LSAFLFPPPPSTFVNVMTVVS+T+LAMAGLSEI GKHLQYSKFWNAN GSKLSA+E
Sbjct: 11 MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
KLSSKYGMLL YTPAFLAGAASFWLFPSGDER FLLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 71 KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MVLKTAIQIS SYF+STAIMIYTQHLSQGLPEPPIDLKNIGVG FL GIIGNFYHHYLLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
QTRKQ +TAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTA+YLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 266
YATRKWYISKFE FPN+VKALLPFVF
Sbjct: 251 YATRKWYISKFENFPNHVKALLPFVF 276
BLAST of Chy10G184120 vs. NCBI nr
Match:
XP_038891781.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])
HSP 1 Score: 474 bits (1221), Expect = 2.65e-167
Identity = 234/266 (87.97%), Postives = 251/266 (94.36%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
MDVLSAFLFPPPPS FVN+MTVVS+TALAMAG+SEINGKHLQYSKFWNAN KLS+E
Sbjct: 34 MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
LSSKYGMLLAYTPAFLAGAASFWLFPS DER+ LLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 94 MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MVL TAIQIS SYF+ST IMIYTQHLSQGLPEPPI+LKNIG+G FLIGIIGNFYHHYLLS
Sbjct: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
+TRK+G+T+Y+IPKGGLFSFVICPHYLFEII +FGFAFISQELYPLCFSIGT++YLAGRS
Sbjct: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 266
YATRKWYISKFE FPN+VKALLPFVF
Sbjct: 274 YATRKWYISKFENFPNHVKALLPFVF 299
BLAST of Chy10G184120 vs. NCBI nr
Match:
XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])
HSP 1 Score: 454 bits (1169), Expect = 6.72e-160
Identity = 222/266 (83.46%), Postives = 242/266 (90.98%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN K +EE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
KLSSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MV TAI+IS SY +ST IMIYTQHLSQGLPEPPIDLKNIG+ FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
+TRK G+T YKIPKGGLFS VICPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 266
YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of Chy10G184120 vs. NCBI nr
Match:
XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])
HSP 1 Score: 454 bits (1167), Expect = 1.36e-159
Identity = 221/266 (83.08%), Postives = 242/266 (90.98%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN K +EE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 120
KLSSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
Query: 121 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 180
MV TAI+IS SY +ST IMIYTQHLSQG+PEPPIDLKNIG+ FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
Query: 181 QTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 240
+TRK G+T YKIPKGGLFS VICPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
Query: 241 YATRKWYISKFETFPNNVKALLPFVF 266
YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of Chy10G184120 vs. TAIR 10
Match:
AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 307.8 bits (787), Expect = 8.8e-84
Identity = 152/268 (56.72%), Postives = 200/268 (74.63%), Query Frame = 0
Query: 1 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGGSKLSAEEY 60
M+++++F++PPPPS +N M+VV ALA G SEI G HL+YSKF ++ + E +
Sbjct: 1 MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60
Query: 61 -KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSS 120
+SS+ GMLL YTPAFLA A+SF++ PS D R LLKSAL LHFFKR+FEVLFIHKYS
Sbjct: 61 GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120
Query: 121 KMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLL 180
M + +A+ IS SYF STA+M+Y+Q+L+ GL EP D+K GV F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180
Query: 181 SQTRKQ-GDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAG 240
++ RK+ G YKIPKGGLF +ICPHYLFEI+ ++ F ISQ +Y F++GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240
Query: 241 RSYATRKWYISKFETFPNNVKALLPFVF 267
RSYATR WY+SKF+ FP ++KAL+PFVF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPFVF 268
BLAST of Chy10G184120 vs. TAIR 10
Match:
AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 71.6 bits (174), Expect = 1.1e-12
Identity = 39/108 (36.11%), Postives = 60/108 (55.56%), Query Frame = 0
Query: 160 IGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFI 219
IG+ F+ G+ N L + +K+ Y IP+GG F V CP+Y E I + G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214
Query: 220 SQELYPLCFSIGTAVYLAGRSYATRKWYISKF-ETFPNNVKALLPFVF 267
+ + F + T L R+ A+ KWYI+KF E +P KA++PFV+
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262
BLAST of Chy10G184120 vs. TAIR 10
Match:
AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 67.0 bits (162), Expect = 2.6e-11
Identity = 48/150 (32.00%), Postives = 72/150 (48.00%), Query Frame = 0
Query: 103 HFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLK-NIG 162
H+FKRI E F+H++S + + +Y+ S I +++ L P DL+ IG
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMKIG 200
Query: 163 VGSFLIGIIGNFYHHYLLSQTR-KQGDTAYKIPKGGLFSFVICPHYLFEIIGYFGFAFIS 222
G L+ + NFY H LL R G Y+IP+G LF+ V C +Y EI + GF +
Sbjct: 201 FGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIAT 260
Query: 223 QELYPLCFSIGTAVYLAGRSYATRKWYISK 251
Q +I V+LA + W + K
Sbjct: 261 Q-------TIAGYVFLAVAALIMTNWALGK 282
BLAST of Chy10G184120 vs. TAIR 10
Match:
AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 57.8 bits (138), Expect = 1.6e-08
Identity = 41/117 (35.04%), Postives = 54/117 (46.15%), Query Frame = 0
Query: 157 LKNIGVGSFLIGIIGNFYHHYLLSQTRKQGDTA--YKIPKGGLFSFVICPHYLFEIIGYF 216
L+ IG FL G I H +L R+ A Y IP G F V CPH+L EI+ Y
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266
Query: 217 GFAFISQ----ELYPLC-FSIGTAVYLAGRSYATRKWYISKFETFPNNVKALLPFVF 267
G S ++ L F Y AG T +WY+ KFE +P + A+ P V+
Sbjct: 267 GLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320
BLAST of Chy10G184120 vs. TAIR 10
Match:
AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 56.6 bits (135), Expect = 3.5e-08
Identity = 41/117 (35.04%), Postives = 54/117 (46.15%), Query Frame = 0
Query: 157 LKNIGVGSFLIGIIGNFYHHYLLSQTRKQGDTA--YKIPKGGLFSFVICPHYLFEIIGYF 216
L+ IG FL G I H +L R+ A Y IP G F V PH+L EI+ Y
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289
Query: 217 GFAFISQ----ELYPLC-FSIGTAVYLAGRSYATRKWYISKFETFPNNVKALLPFVF 267
G S ++ L F Y AG T +WY+ KFE +P N A+ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q28891 | 4.8e-18 | 40.19 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1... | [more] |
P18405 | 1.4e-17 | 39.25 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S... | [more] |
Q55C17 | 4.0e-17 | 29.73 | Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn... | [more] |
Q2QDF6 | 6.9e-17 | 31.50 | Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1 | [more] |
O18765 | 1.5e-16 | 37.96 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BFS0 | 4.8e-138 | 93.23 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348... | [more] |
A0A0A0KTC7 | 1.5e-131 | 91.35 | S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128... | [more] |
A0A6J1GZA3 | 1.0e-124 | 83.46 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A6J1J6R3 | 1.8e-124 | 83.08 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A6J1DAI6 | 6.3e-122 | 81.20 | very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
Match Name | E-value | Identity | Description | |
XP_004135052.2 | 6.03e-186 | 98.12 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothet... | [more] |
XP_008446722.1 | 2.89e-177 | 93.23 | PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo] | [more] |
XP_038891781.1 | 2.65e-167 | 87.97 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida] | [more] |
XP_022957108.1 | 6.72e-160 | 83.46 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata] | [more] |
XP_022984951.1 | 1.36e-159 | 83.08 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
AT5G16010.1 | 8.8e-84 | 56.72 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G38050.1 | 1.1e-12 | 36.11 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT3G55360.1 | 2.6e-11 | 32.00 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT1G72590.1 | 1.6e-08 | 35.04 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G16530.1 | 3.5e-08 | 35.04 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |