Chy10G182430 (gene) Cucumber (hystrix) v1

Overview
NameChy10G182430
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptiont-SNARE coiled-coil homology domain-containing protein
LocationchrH10: 15703284 .. 15704841 (-)
RNA-Seq ExpressionChy10G182430
SyntenyChy10G182430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAGTTTCATGAAATCTCCCGCGAAAGTTACTAAGCAGAATTCTGTGGATCCTGAACTATCAACCGGATCTGGCACTAATCCTTTTGATTCAGATACTGGGCCTGATGCCAAACAAACTCTTAATGCCGCAAGAAGAACTTCTTCCGAGCCTGTGCTCCCTATGCCCAAGGCTAACCCTTTTGATGATGATGATGATAATGGTTTTGTTGGAAGAAAAGGGACAGCAACTTCTTCAGGCTCTAAGGATAGATACAAAAATGATTTTCGCGACTCCGGAGGACTTGAGAACCAGAGTGTGCAGGAATTGGAAAACTACGCTGTGTATAAAGCTGAGGAGACCACGAAATCTGTTAATAACTGCTTGAAGATCGCTGAGGATATAAGGGAAGATGCTACCAAGACCCTTGACATGTTGCATAAGCAGGGTGAGCAAATTGAGAGGACCCATAGGATGGCTGCTGATATGGATAAAGATTTAAGTAAGGTAGGTTCCCTAGTTTCCATCAGACGTGTTTTCAACATATTTTCTGTCACATGTTGTCCTGAAATTTCTAGTTGCCCATTTTGATCGGATATATTTGGCTTGCAAGTTTTTGTGATTATTAGATTGTGGATGTCATACTCTGTAGGATTAAAAATAGTTAAGAACGAGGAACAGTTTAGCTTGCTAGGAATTCTATAAGTAAGCCAGAGAAGGAAAGAACATATTTTTGTCTTGCTTTTTCATTGTTAATGTGTCCTTTTCTCATGATACCTTGATTGATCCTCCATGTGTTTCTTAATAGTTCATTTTTTCATTATAATTAATCCAGGGTGAGAAGCTTCTGAATAATCTTGGTGGCATGTTCTCTAAGCCTTGGAAGCCAAAGAAGACCAAAGAAATTACAGGCCCTCTTATAACAGCAGGTTGTTACATCTTCGTTGATTCTTTTGCTCACTACACGCTGACATTTTTGTATCTATCTTACTGCCACTCCCATTTTTATCTGCTATTGACTAATGTCAATTTTGGCTGGCCCCAGATCATTCCTCTGGGAAAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTTTATCTACGGGCAAAAAACAGTCAGCTACTAGAACACCACCCTCTGAACCATCAGGCGCAATACAAAAAGTTGAGGTGAGAGCATCAGTTGTTATCATTTCACTATCTTAAAGAGAGCATTCTCATGGTTCCAAATCTAATCAATATGTAAGAATGTTTAAAAACACAGGTAGAGAAGGAAAAGCAAGATGATGCGCTCTCAGATTTAAGTAATATCTTGGGCGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTGGACAGGTCACACATGCCATCCTCTTTTCTCAATTTAAACAATGTTGTTGTATAAACATCTCATCAATCAGTTAGCTTAGTACATAGTTGTGAATTGATACTTTCTTAAAAATGCAGGCAAAACAAAGCTCTGGATCATCTAAGCGGTGACGTTGACGAGCTGAACTCTAGAGTGAAAGGTGCCAATCAACGAGCTCGCCATTTGATCGGGAAGTAA

mRNA sequence

ATGTTTAGTTTCATGAAATCTCCCGCGAAAGTTACTAAGCAGAATTCTGTGGATCCTGAACTATCAACCGGATCTGGCACTAATCCTTTTGATTCAGATACTGGGCCTGATGCCAAACAAACTCTTAATGCCGCAAGAAGAACTTCTTCCGAGCCTGTGCTCCCTATGCCCAAGGCTAACCCTTTTGATGATGATGATGATAATGGTTTTGTTGGAAGAAAAGGGACAGCAACTTCTTCAGGCTCTAAGGATAGATACAAAAATGATTTTCGCGACTCCGGAGGACTTGAGAACCAGAGTGTGCAGGAATTGGAAAACTACGCTGTGTATAAAGCTGAGGAGACCACGAAATCTGTTAATAACTGCTTGAAGATCGCTGAGGATATAAGGGAAGATGCTACCAAGACCCTTGACATGTTGCATAAGCAGGGTGAGCAAATTGAGAGGACCCATAGGATGGCTGCTGATATGGATAAAGATTTAAGTAAGGGTGAGAAGCTTCTGAATAATCTTGGTGGCATGTTCTCTAAGCCTTGGAAGCCAAAGAAGACCAAAGAAATTACAGGCCCTCTTATAACAGCAGATCATTCCTCTGGGAAAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTTTATCTACGGGCAAAAAACAGTCAGCTACTAGAACACCACCCTCTGAACCATCAGGCGCAATACAAAAAGTTGAGGTAGAGAAGGAAAAGCAAGATGATGCGCTCTCAGATTTAAGTAATATCTTGGGCGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTGGACAGGCAAAACAAAGCTCTGGATCATCTAAGCGGTGACGTTGACGAGCTGAACTCTAGAGTGAAAGGTGCCAATCAACGAGCTCGCCATTTGATCGGGAAGTAA

Coding sequence (CDS)

ATGTTTAGTTTCATGAAATCTCCCGCGAAAGTTACTAAGCAGAATTCTGTGGATCCTGAACTATCAACCGGATCTGGCACTAATCCTTTTGATTCAGATACTGGGCCTGATGCCAAACAAACTCTTAATGCCGCAAGAAGAACTTCTTCCGAGCCTGTGCTCCCTATGCCCAAGGCTAACCCTTTTGATGATGATGATGATAATGGTTTTGTTGGAAGAAAAGGGACAGCAACTTCTTCAGGCTCTAAGGATAGATACAAAAATGATTTTCGCGACTCCGGAGGACTTGAGAACCAGAGTGTGCAGGAATTGGAAAACTACGCTGTGTATAAAGCTGAGGAGACCACGAAATCTGTTAATAACTGCTTGAAGATCGCTGAGGATATAAGGGAAGATGCTACCAAGACCCTTGACATGTTGCATAAGCAGGGTGAGCAAATTGAGAGGACCCATAGGATGGCTGCTGATATGGATAAAGATTTAAGTAAGGGTGAGAAGCTTCTGAATAATCTTGGTGGCATGTTCTCTAAGCCTTGGAAGCCAAAGAAGACCAAAGAAATTACAGGCCCTCTTATAACAGCAGATCATTCCTCTGGGAAAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTTTATCTACGGGCAAAAAACAGTCAGCTACTAGAACACCACCCTCTGAACCATCAGGCGCAATACAAAAAGTTGAGGTAGAGAAGGAAAAGCAAGATGATGCGCTCTCAGATTTAAGTAATATCTTGGGCGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTGGACAGGCAAAACAAAGCTCTGGATCATCTAAGCGGTGACGTTGACGAGCTGAACTCTAGAGTGAAAGGTGCCAATCAACGAGCTCGCCATTTGATCGGGAAGTAA

Protein sequence

MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKANPFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHLIGK*
Homology
BLAST of Chy10G182430 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 8.2e-91
Identity = 186/302 (61.59%), Postives = 217/302 (71.85%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MF F KSP                 G N   +++  +   T+ A RRTSSEP+L  P   
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITP--- 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
            FDDD                  D+YKN F DSGGL++Q+ +ELE YAVYKAEETTK VN
Sbjct: 61  DFDDD------------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180
           NCLKIAEDIR D  +TL+MLH+QGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKPWK
Sbjct: 121 NCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWK 180

Query: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEK 240
           PKKTK ITGP+IT D  S K+EN+KE+REKLGL   K +S+++    +P+ A+QKVE EK
Sbjct: 181 PKKTKNITGPMITPDKPSKKSENHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEK 240

Query: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHLI 300
            KQDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL  DVDELNSRV+GANQRARHL+
Sbjct: 241 AKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLL 263

Query: 301 GK 303
            K
Sbjct: 301 SK 263

BLAST of Chy10G182430 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.4e-87
Identity = 184/304 (60.53%), Postives = 221/304 (72.70%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MF   KSPA + K NSVD +    S  NPFDSD   D K TLN ++RT+SEP L     N
Sbjct: 1   MFGLRKSPANLPKHNSVDLK---SSKPNPFDSDDESDNKHTLNPSKRTTSEPSL-ADMTN 60

Query: 61  PFDDDD-DNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSV 120
           PF  +    G       +  S SK +YKN+FRDSGG+ENQSVQELE YAVYKAEETTKSV
Sbjct: 61  PFGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSV 120

Query: 121 NNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPW 180
             CLK+AEDIR DAT+TL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMFSK W
Sbjct: 121 QGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTW 180

Query: 181 KPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKK-QSATRTPPSEPSGAIQKVEV 240
           KPKKT+ I GP++T D S  +  N+ E+REKLGL++  + QS TR P  E + A Q+VE+
Sbjct: 181 KPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEM 240

Query: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARH 300
           EK KQDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL  DVDELN RV+ +NQR R 
Sbjct: 241 EKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRR 300

Query: 301 LIGK 303
           L+GK
Sbjct: 301 LLGK 300

BLAST of Chy10G182430 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.1e-58
Identity = 127/243 (52.26%), Postives = 170/243 (69.96%), Query Frame = 0

Query: 60  NPFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSV 119
           NPFDDDDD+  V           + R+ +  + SGG ENQ+VQELE+YAVY +EETTK+V
Sbjct: 24  NPFDDDDDDKEV-----------EKRFTSSLKPSGGKENQTVQELESYAVYNSEETTKTV 83

Query: 120 NNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPW 179
             CLK+AE+IR DA+KTL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+FS+ W
Sbjct: 84  QGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVFSRTW 143

Query: 180 KPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVE 239
           KPKK++ ITGP+IT   S  +   + + REKLGL+   K  +   P  E   A QK ++ 
Sbjct: 144 KPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP--EAVDAYQKTQI- 203

Query: 240 KEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHL 299
             KQD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL  + DELN RVK +NQRAR+L
Sbjct: 204 -AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRARYL 251

Query: 300 IGK 303
           + K
Sbjct: 264 LRK 251

BLAST of Chy10G182430 vs. ExPASy Swiss-Prot
Match: P36977 (Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 58/208 (27.88%), Postives = 106/208 (50.96%), Query Frame = 0

Query: 101 VQELENYAVYKAEETTKSVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKD 160
           + +++  A   A+E+ +S    L++ E+ ++   +TL ML +QGEQ+ER       ++KD
Sbjct: 11  LSDMQQRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 161 LSKGEKLLNNLG---GMFSKPWKPKKTKEITGPLITADHSSGKTENNK----EQREKLGL 220
           +   EK LN+LG   G+ S P    K+    G     ++  G   +      ++RE++ +
Sbjct: 71  MKDAEKNLNDLGKFCGLCSCPCNKMKS---GGSKAWGNNQDGVVASQPARVVDEREQMAI 130

Query: 221 STGKKQSATRTPPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNK 280
           S G  +  T              +  + + D+ L  +  I+G+L+ MA+DMG+E+D QN+
Sbjct: 131 SGGFIRRVTD-------------DARENEMDENLEQVGGIIGNLRHMALDMGNEIDTQNR 190

Query: 281 ALDHLSGDVDELNSRVKGANQRARHLIG 302
            +D +    D   +R+  ANQRA  ++G
Sbjct: 191 QIDRIMEKADSNKTRIDEANQRATKMLG 202

BLAST of Chy10G182430 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.6e-14
Identity = 57/208 (27.40%), Postives = 107/208 (51.44%), Query Frame = 0

Query: 101 VQELENYAVYKAEETTKSVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKD 160
           ++E++  A   A+E+ +S    L++ E+ ++   +TL ML +QGEQ+ER       ++KD
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 161 LSKGEKLLNNLG---GMFSKPWKPKKTKEITGPLITADHSSGKTENNK----EQREKLGL 220
           + + EK L +LG   G+   P    K+ +        ++  G   +      ++RE++ +
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSD-AYKKAWGNNQDGVVASQPARVVDEREQMAI 130

Query: 221 STGKKQSATRTPPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNK 280
           S G  +  T              +  + + D+ L  +S I+G+L+ MA+DMG+E+D QN+
Sbjct: 131 SGGFIRRVTN-------------DARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNR 190

Query: 281 ALDHLSGDVDELNSRVKGANQRARHLIG 302
            +D +    D   +R+  ANQRA  ++G
Sbjct: 191 QIDRIMEKADSNKTRIDEANQRATKMLG 204

BLAST of Chy10G182430 vs. ExPASy TrEMBL
Match: A0A0A0KQT5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606590 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.6e-161
Identity = 299/302 (99.01%), Postives = 300/302 (99.34%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
           PFDDDDD GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN
Sbjct: 61  PFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180
           NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK
Sbjct: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180

Query: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEK 240
           PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSAT+TPPSEPSGAIQKVEVEK
Sbjct: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQKVEVEK 240

Query: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHLI 300
           EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRARHLI
Sbjct: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHLI 300

Query: 301 GK 303
           GK
Sbjct: 301 GK 302

BLAST of Chy10G182430 vs. ExPASy TrEMBL
Match: A0A1S3BF13 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103489173 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.4e-157
Identity = 294/302 (97.35%), Postives = 298/302 (98.68%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLP+P AN
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPNAN 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
           PFDDDD  GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN
Sbjct: 61  PFDDDD--GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180
           NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK
Sbjct: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180

Query: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEK 240
           PKKTKEITGPLITADHSSGKTENNK+QREKLGLSTGKKQSAT+TPPSEPSGA+QKVEVEK
Sbjct: 181 PKKTKEITGPLITADHSSGKTENNKKQREKLGLSTGKKQSATKTPPSEPSGAMQKVEVEK 240

Query: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHLI 300
           EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRARHLI
Sbjct: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHLI 300

Query: 301 GK 303
           GK
Sbjct: 301 GK 300

BLAST of Chy10G182430 vs. ExPASy TrEMBL
Match: A0A5D3CF89 (Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00020 PE=4 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.3e-151
Identity = 292/337 (86.65%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLP+P AN
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPNAN 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
           PFDDDD  GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN
Sbjct: 61  PFDDDD--GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLS------------------ 180
           NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDL+                  
Sbjct: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLNIFGLQVFVMIRLWMSYSV 180

Query: 181 -----------------KGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTENNK 240
                             GEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTENNK
Sbjct: 181 GLKLVKNEQPFSLLGILYGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTENNK 240

Query: 241 EQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMG 300
           +QREKLGLSTGKKQSAT+TPPSEPSGA+QKVEVEKEKQDDALSDLSNILGDLKSMAVDMG
Sbjct: 241 KQREKLGLSTGKKQSATKTPPSEPSGAMQKVEVEKEKQDDALSDLSNILGDLKSMAVDMG 300

Query: 301 SELDRQNKALDHLSGDVDELNSRVKGANQRARHLIGK 303
           SELDRQNKALDHLS DVDELNSRVKGANQRARHLIGK
Sbjct: 301 SELDRQNKALDHLSDDVDELNSRVKGANQRARHLIGK 335

BLAST of Chy10G182430 vs. ExPASy TrEMBL
Match: A0A6J1J9F6 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111482502 PE=4 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 7.6e-148
Identity = 278/305 (91.15%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMP--- 60
           MFSFMKSPAKVTKQNSVD ELS GSGTNPFDSD+GP+AKQTLNAARRTSSEPVL +P   
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  KANPFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
            AN FDDDDD+GFVG++GTATS+ SKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDATKTLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVE 240
           PWKPKKTKEITGPLITADHSSG+TENNKEQREKLG+STGKKQSAT+TPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 HLIGK 303
           HL+GK
Sbjct: 301 HLVGK 304

BLAST of Chy10G182430 vs. ExPASy TrEMBL
Match: A0A6J1H000 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111458786 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.6e-145
Identity = 275/305 (90.16%), Postives = 291/305 (95.41%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMP--- 60
           MFSFMKSPAKVTKQNSVD ELS GSGTNPFDSD+GP+AKQTLNAARRTSSEPVL +P   
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLIIPNSI 60

Query: 61  KANPFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
            AN FDDDDD+GFVG++ TATS+ SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK
Sbjct: 61  SANDFDDDDDDGFVGKRRTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV+NCLKIAEDIREDATKTLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVE 240
           PWKPKKTKEITGPLITADHSSG+TENNKEQREKLG+STGKKQS T+TPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRAR 300
            +KE QDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 HLIGK 303
           HL+GK
Sbjct: 301 HLVGK 304

BLAST of Chy10G182430 vs. NCBI nr
Match: XP_004135151.1 (putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_031740914.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >KGN51968.1 hypothetical protein Csa_009347 [Cucumis sativus])

HSP 1 Score: 581 bits (1498), Expect = 7.77e-209
Identity = 299/302 (99.01%), Postives = 300/302 (99.34%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
           PFDDDDD GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN
Sbjct: 61  PFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180
           NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK
Sbjct: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180

Query: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEK 240
           PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSAT+TPPSEPSGAIQKVEVEK
Sbjct: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQKVEVEK 240

Query: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHLI 300
           EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRARHLI
Sbjct: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHLI 300

Query: 301 GK 302
           GK
Sbjct: 301 GK 302

BLAST of Chy10G182430 vs. NCBI nr
Match: XP_008446422.1 (PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo])

HSP 1 Score: 568 bits (1465), Expect = 7.73e-204
Identity = 294/302 (97.35%), Postives = 298/302 (98.68%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLP+P AN
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPNAN 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
           PFDDDD  GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN
Sbjct: 61  PFDDDD--GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180
           NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK
Sbjct: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180

Query: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEK 240
           PKKTKEITGPLITADHSSGKTENNK+QREKLGLSTGKKQSAT+TPPSEPSGA+QKVEVEK
Sbjct: 181 PKKTKEITGPLITADHSSGKTENNKKQREKLGLSTGKKQSATKTPPSEPSGAMQKVEVEK 240

Query: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHLI 300
           EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRARHLI
Sbjct: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHLI 300

Query: 301 GK 302
           GK
Sbjct: 301 GK 300

BLAST of Chy10G182430 vs. NCBI nr
Match: KAA0034463.1 (putative SNAP25-like proteinous protein SNAP30 [Cucumis melo var. makuwa] >TYK09016.1 putative SNAP25-like proteinous protein SNAP30 [Cucumis melo var. makuwa])

HSP 1 Score: 547 bits (1409), Expect = 9.57e-195
Identity = 292/337 (86.65%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLP+P AN
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPNAN 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
           PFDDDD  GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN
Sbjct: 61  PFDDDD--GFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLS------------------ 180
           NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDL+                  
Sbjct: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLNIFGLQVFVMIRLWMSYSV 180

Query: 181 -----------------KGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTENNK 240
                             GEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTENNK
Sbjct: 181 GLKLVKNEQPFSLLGILYGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTENNK 240

Query: 241 EQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMG 300
           +QREKLGLSTGKKQSAT+TPPSEPSGA+QKVEVEKEKQDDALSDLSNILGDLKSMAVDMG
Sbjct: 241 KQREKLGLSTGKKQSATKTPPSEPSGAMQKVEVEKEKQDDALSDLSNILGDLKSMAVDMG 300

Query: 301 SELDRQNKALDHLSGDVDELNSRVKGANQRARHLIGK 302
           SELDRQNKALDHLS DVDELNSRVKGANQRARHLIGK
Sbjct: 301 SELDRQNKALDHLSDDVDELNSRVKGANQRARHLIGK 335

BLAST of Chy10G182430 vs. NCBI nr
Match: XP_038891847.1 (putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 putative SNAP25 homologous protein SNAP30 [Benincasa hispida])

HSP 1 Score: 542 bits (1397), Expect = 2.07e-193
Identity = 284/305 (93.11%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKA- 60
           MF FMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVL MP + 
Sbjct: 1   MFGFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLIMPNSM 60

Query: 61  --NPFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
             NPFDDDD+ GFVGR+GTATSS SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK
Sbjct: 61  SSNPFDDDDE-GFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVE 240
           PWKPKKT+EITGPLITAD+SSG+TENNKEQREKLG+STGKKQSAT+TPPSE SGA+QKVE
Sbjct: 181 PWKPKKTREITGPLITADNSSGRTENNKEQREKLGVSTGKKQSATQTPPSEQSGAMQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRAR 300
            EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRAR
Sbjct: 241 AEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 HLIGK 302
            LIGK
Sbjct: 301 KLIGK 304

BLAST of Chy10G182430 vs. NCBI nr
Match: XP_022984084.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita maxima])

HSP 1 Score: 536 bits (1381), Expect = 5.67e-191
Identity = 278/305 (91.15%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPK-- 60
           MFSFMKSPAKVTKQNSVD ELS GSGTNPFDSD+GP+AKQTLNAARRTSSEPVL +P   
Sbjct: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  -ANPFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
            AN FDDDDD+GFVG++GTATS+ SKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDATKTLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVE 240
           PWKPKKTKEITGPLITADHSSG+TENNKEQREKLG+STGKKQSAT+TPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLS DVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 HLIGK 302
           HL+GK
Sbjct: 301 HLVGK 304

BLAST of Chy10G182430 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 335.1 bits (858), Expect = 5.8e-92
Identity = 186/302 (61.59%), Postives = 217/302 (71.85%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MF F KSP                 G N   +++  +   T+ A RRTSSEP+L  P   
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITP--- 60

Query: 61  PFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVN 120
            FDDD                  D+YKN F DSGGL++Q+ +ELE YAVYKAEETTK VN
Sbjct: 61  DFDDD------------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVN 120

Query: 121 NCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWK 180
           NCLKIAEDIR D  +TL+MLH+QGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKPWK
Sbjct: 121 NCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWK 180

Query: 181 PKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVEK 240
           PKKTK ITGP+IT D  S K+EN+KE+REKLGL   K +S+++    +P+ A+QKVE EK
Sbjct: 181 PKKTKNITGPMITPDKPSKKSENHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEK 240

Query: 241 EKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHLI 300
            KQDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL  DVDELNSRV+GANQRARHL+
Sbjct: 241 AKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLL 263

Query: 301 GK 303
            K
Sbjct: 301 SK 263

BLAST of Chy10G182430 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 324.3 bits (830), Expect = 1.0e-88
Identity = 184/304 (60.53%), Postives = 221/304 (72.70%), Query Frame = 0

Query: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKAN 60
           MF   KSPA + K NSVD +    S  NPFDSD   D K TLN ++RT+SEP L     N
Sbjct: 1   MFGLRKSPANLPKHNSVDLK---SSKPNPFDSDDESDNKHTLNPSKRTTSEPSL-ADMTN 60

Query: 61  PFDDDD-DNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSV 120
           PF  +    G       +  S SK +YKN+FRDSGG+ENQSVQELE YAVYKAEETTKSV
Sbjct: 61  PFGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSV 120

Query: 121 NNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPW 180
             CLK+AEDIR DAT+TL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMFSK W
Sbjct: 121 QGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTW 180

Query: 181 KPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKK-QSATRTPPSEPSGAIQKVEV 240
           KPKKT+ I GP++T D S  +  N+ E+REKLGL++  + QS TR P  E + A Q+VE+
Sbjct: 181 KPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEM 240

Query: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARH 300
           EK KQDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL  DVDELN RV+ +NQR R 
Sbjct: 241 EKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRR 300

Query: 301 LIGK 303
           L+GK
Sbjct: 301 LLGK 300

BLAST of Chy10G182430 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 226.5 bits (576), Expect = 2.9e-59
Identity = 127/243 (52.26%), Postives = 170/243 (69.96%), Query Frame = 0

Query: 60  NPFDDDDDNGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSV 119
           NPFDDDDD+  V           + R+ +  + SGG ENQ+VQELE+YAVY +EETTK+V
Sbjct: 24  NPFDDDDDDKEV-----------EKRFTSSLKPSGGKENQTVQELESYAVYNSEETTKTV 83

Query: 120 NNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPW 179
             CLK+AE+IR DA+KTL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+FS+ W
Sbjct: 84  QGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVFSRTW 143

Query: 180 KPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTPPSEPSGAIQKVEVE 239
           KPKK++ ITGP+IT   S  +   + + REKLGL+   K  +   P  E   A QK ++ 
Sbjct: 144 KPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP--EAVDAYQKTQI- 203

Query: 240 KEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNSRVKGANQRARHL 299
             KQD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL  + DELN RVK +NQRAR+L
Sbjct: 204 -AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRARYL 251

Query: 300 IGK 303
           + K
Sbjct: 264 LRK 251

BLAST of Chy10G182430 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 45.4 bits (106), Expect = 9.2e-05
Identity = 53/197 (26.90%), Postives = 85/197 (43.15%), Query Frame = 0

Query: 108 AVYKAEETTKSVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKL 167
           A+ KAE  TK  N    +A +     TK      +  E++ +  R+A    K L+  E  
Sbjct: 50  ALEKAELVTKEKNRAAAVAMNAEIRRTKA-----RLSEEVPKLQRLAVKRVKGLTTEELA 109

Query: 168 LNNLGGMFSKPWKPKKTKEIT--GPLITADHSSGKTENNKEQREKLGLSTGKKQSATRTP 227
             N   + + P + +   + T  GP  T+  +   T +  + +     S G+        
Sbjct: 110 ARN-DLVLALPARIEAIPDGTAGGPKSTSAWTPSSTTSRPDIKFD---SDGRFDDDYFQE 169

Query: 228 PSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDEL 287
            +E S   Q+ E+ K KQ+  L  +S  L  LK+MA DM  ELDRQ   +D +   VD  
Sbjct: 170 SNESSQFRQEYEMRKIKQEQGLDMISEGLDALKNMASDMNEELDRQVPLMDEIDTKVDRA 229

Query: 288 NSRVKGANQRARHLIGK 303
            S +K  N R +  + +
Sbjct: 230 TSDLKNTNVRLKDTVNQ 237

BLAST of Chy10G182430 vs. TAIR 10
Match: AT3G45280.1 (syntaxin of plants 72 )

HSP 1 Score: 42.4 bits (98), Expect = 7.8e-04
Identity = 25/70 (35.71%), Postives = 39/70 (55.71%), Query Frame = 0

Query: 228 EPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSGDVDELNS 287
           E S   Q+ E+ ++KQD+ L  +S  L  LK++A DM  ELD+Q   ++ +   VD   S
Sbjct: 164 ESSQFRQEYEMRRKKQDEGLDIISEGLDALKNLARDMNEELDKQVPLMEEMETKVDGATS 223

Query: 288 RVKGANQRAR 298
            +K  N R +
Sbjct: 224 DLKNTNVRLK 233

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMG88.2e-9161.59Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA... [more]
Q9S7P91.4e-8760.53SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ... [more]
Q9SD964.1e-5852.26SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ... [more]
P369771.2e-1427.88Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=... [more]
Q17QQ33.6e-1427.40Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQT51.6e-16199.01t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365... [more]
A0A1S3BF131.4e-15797.35putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10348917... [more]
A0A5D3CF894.3e-15186.65Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1J9F67.6e-14891.15putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1H0001.6e-14590.16putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_004135151.17.77e-20999.01putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 puta... [more]
XP_008446422.17.73e-20497.35PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo][more]
KAA0034463.19.57e-19586.65putative SNAP25-like proteinous protein SNAP30 [Cucumis melo var. makuwa] >TYK09... [more]
XP_038891847.12.07e-19393.11putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 pu... [more]
XP_022984084.15.67e-19191.15putative SNAP25 homologous protein SNAP30 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G13890.15.8e-9261.59soluble N-ethylmaleimide-sensitive factor adaptor protein 30 [more]
AT5G61210.11.0e-8860.53soluble N-ethylmaleimide-sensitive factor adaptor protein 33 [more]
AT5G07880.12.9e-5952.26synaptosomal-associated protein SNAP25-like 29 [more]
AT3G09740.19.2e-0526.90syntaxin of plants 71 [more]
AT3G45280.17.8e-0435.71syntaxin of plants 72 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 268..295
NoneNo IPR availableGENE3D1.20.5.110coord: 234..301
e-value: 1.8E-22
score: 81.3
NoneNo IPR availableGENE3D1.20.5.110coord: 95..173
e-value: 4.0E-22
score: 80.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..213
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..244
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 214..229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..48
NoneNo IPR availablePANTHERPTHR19305SYNAPTOSOMAL ASSOCIATED PROTEINcoord: 1..302
NoneNo IPR availablePANTHERPTHR19305:SF25SNAP25 HOMOLOGOUS PROTEIN SNAP30-RELATEDcoord: 1..302
NoneNo IPR availableCDDcd15841SNARE_Qccoord: 240..298
e-value: 3.00615E-15
score: 66.8132
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 233..299
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 99..170
IPR000727Target SNARE coiled-coil homology domainSMARTSM00397tSNARE_6coord: 104..171
e-value: 0.26
score: 20.3
coord: 232..299
e-value: 1.9E-10
score: 50.7
IPR000727Target SNARE coiled-coil homology domainPROSITEPS50192T_SNAREcoord: 237..299
score: 16.615763
IPR044766NPSN/SNAP25-like, N-terminal SNARE domainCDDcd15861SNARE_SNAP25N_23N_29N_SEC9Ncoord: 106..170
e-value: 1.19258E-21
score: 84.5471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G182430.1Chy10G182430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061025 membrane fusion
biological_process GO:0015031 protein transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005484 SNAP receptor activity