Chy10G182170 (gene) Cucumber (hystrix) v1

Overview
NameChy10G182170
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMitochondrial uncoupling protein
LocationchrH10: 15557977 .. 15559455 (+)
RNA-Seq ExpressionChy10G182170
SyntenyChy10G182170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCAACGCACAATCCCTACACCAAATTGGTACTCACCGGCCTCTCCGCCATGGTGGCGGAGTCCGCCACCTTCCCCATAGACCTCACTAAGACCAGGCTTCAGCTCCACGGCGAGTCTTCCTCCTCCTCTCGCTCCACAAATGCCTTCAGACTCGCTTCAGCCATTGTCAAGGATCAAGGTCCTTTTGCCCTCTACAAGGGCTTGTCTCCCGCGATTCTCAGGCACCTTTTCTATACGCCCATACGAATCGTTGGATACGAACACCTCCGATCTCTCTTCCTTGCGTCCGATGGTGGCTCTGTTTCCTTCCATTCCAAGGCTCTTGTCGGTGGAATCTCCGGCTCCATTGCTCAGGTTCCAATTTTTCCACATTAGCATTTTATTGAGGCTTTGTTTAACGATATGGTACATTTAAGATATTTGTATTTGCCAATGAACTGTATGATCTCTAGTATTTGTGCCAATCTTCCCAATTGTGACATGATGGAATTGATTGATGTTATAGAATTTTTTCATTGCTTTGAATTTTGTGAATCTAGGATGAGTCTGAAAATTACAGCCATTATCTGAACGGTCAATTGTGAGCTTGAAAATTGGCACAACATATACATTTTGCTTTTAGTACACACCTCAACTATTTTTATTAATATCACGGGACAACTCATCTAGCTCTATCTCAAGAACACTTGTTGGATATTAAATCCATGATAGGCAGCTACCATGGATTGAATCATTCTCTTATTAACCCTTTATCATATCCAAGCTTTTCATACGCGACTAGAGCAACCAAGGATAGTTACAAAATATATGTTTTTCTATAAACCCATATGTTAAACTATCACTAAATTCAAAGTTATTGATATTCTTAACATGCTGATACTACTTTGCTAAATATCAGTATCTTTTTTGTGTTTACCATTAGCATTTCAGGTAGTGGCAAGTCCTGCTGATCTTGTTAAAGTGAGGATGCAAGCTGATGGGCGTCTAATAAGTCAAGGTCTGCAACCTAGATACTCAGGGCCATTCGATGCCTTAACCAAGATAGTGCGTGGAGAAGGGGTTGTTGGACTTTGGAAAGGGGTTGTTCCAAATGTTCAAAGAGCATTTTTGGTTAACATGGGAGAATTAGCCTGCTACGATCATGCTAAACGTTTTGTTATTCAGAATCAATTAGCTGGAGATAATATTTTTGGCCACACTTGTGCTTCTGTAATTTCAGGTCTGTGTGCAACTGCTTTGAGTTGTCCAGCTGATGTTGTAAAGACAAGAATGATGAATCAAGCTGCTAGCAAGGAAGGAATTACCAAGTACAACAGCTCTTATGATTGTCTTGTGAAGACTGTTAAAGTTGAAGGATTGAGAGCTTTGTGGAAGGGGTTTTTTCCTACTTGGGCCAGGCTTGGTCCTTGGCAATTTGTCTTCTGGGTGTCTTATGAGAAGTTTCGTAAACTTGCTGGGTTATCTTCCTTCTAG

mRNA sequence

ATGGAAGCAACGCACAATCCCTACACCAAATTGGTACTCACCGGCCTCTCCGCCATGGTGGCGGAGTCCGCCACCTTCCCCATAGACCTCACTAAGACCAGGCTTCAGCTCCACGGCGAGTCTTCCTCCTCCTCTCGCTCCACAAATGCCTTCAGACTCGCTTCAGCCATTGTCAAGGATCAAGGTCCTTTTGCCCTCTACAAGGGCTTGTCTCCCGCGATTCTCAGGCACCTTTTCTATACGCCCATACGAATCGTTGGATACGAACACCTCCGATCTCTCTTCCTTGCGTCCGATGGTGGCTCTGTTTCCTTCCATTCCAAGGCTCTTGTCGGTGGAATCTCCGGCTCCATTGCTCAGGTAGTGGCAAGTCCTGCTGATCTTGTTAAAGTGAGGATGCAAGCTGATGGGCGTCTAATAAGTCAAGGTCTGCAACCTAGATACTCAGGGCCATTCGATGCCTTAACCAAGATAGTGCGTGGAGAAGGGGTTGTTGGACTTTGGAAAGGGGTTGTTCCAAATGTTCAAAGAGCATTTTTGGTTAACATGGGAGAATTAGCCTGCTACGATCATGCTAAACGTTTTGTTATTCAGAATCAATTAGCTGGAGATAATATTTTTGGCCACACTTGTGCTTCTGTAATTTCAGGTCTGTGTGCAACTGCTTTGAGTTGTCCAGCTGATGTTGTAAAGACAAGAATGATGAATCAAGCTGCTAGCAAGGAAGGAATTACCAAGTACAACAGCTCTTATGATTGTCTTGTGAAGACTGTTAAAGTTGAAGGATTGAGAGCTTTGTGGAAGGGGTTTTTTCCTACTTGGGCCAGGCTTGGTCCTTGGCAATTTGTCTTCTGGGTGTCTTATGAGAAGTTTCGTAAACTTGCTGGGTTATCTTCCTTCTAG

Coding sequence (CDS)

ATGGAAGCAACGCACAATCCCTACACCAAATTGGTACTCACCGGCCTCTCCGCCATGGTGGCGGAGTCCGCCACCTTCCCCATAGACCTCACTAAGACCAGGCTTCAGCTCCACGGCGAGTCTTCCTCCTCCTCTCGCTCCACAAATGCCTTCAGACTCGCTTCAGCCATTGTCAAGGATCAAGGTCCTTTTGCCCTCTACAAGGGCTTGTCTCCCGCGATTCTCAGGCACCTTTTCTATACGCCCATACGAATCGTTGGATACGAACACCTCCGATCTCTCTTCCTTGCGTCCGATGGTGGCTCTGTTTCCTTCCATTCCAAGGCTCTTGTCGGTGGAATCTCCGGCTCCATTGCTCAGGTAGTGGCAAGTCCTGCTGATCTTGTTAAAGTGAGGATGCAAGCTGATGGGCGTCTAATAAGTCAAGGTCTGCAACCTAGATACTCAGGGCCATTCGATGCCTTAACCAAGATAGTGCGTGGAGAAGGGGTTGTTGGACTTTGGAAAGGGGTTGTTCCAAATGTTCAAAGAGCATTTTTGGTTAACATGGGAGAATTAGCCTGCTACGATCATGCTAAACGTTTTGTTATTCAGAATCAATTAGCTGGAGATAATATTTTTGGCCACACTTGTGCTTCTGTAATTTCAGGTCTGTGTGCAACTGCTTTGAGTTGTCCAGCTGATGTTGTAAAGACAAGAATGATGAATCAAGCTGCTAGCAAGGAAGGAATTACCAAGTACAACAGCTCTTATGATTGTCTTGTGAAGACTGTTAAAGTTGAAGGATTGAGAGCTTTGTGGAAGGGGTTTTTTCCTACTTGGGCCAGGCTTGGTCCTTGGCAATTTGTCTTCTGGGTGTCTTATGAGAAGTTTCGTAAACTTGCTGGGTTATCTTCCTTCTAG

Protein sequence

MEATHNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF*
Homology
BLAST of Chy10G182170 vs. ExPASy Swiss-Prot
Match: Q9XI74 (Mitochondrial uncoupling protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUMP3 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.3e-116
Identity = 209/296 (70.61%), Postives = 243/296 (82.09%), Query Frame = 0

Query: 9   TKLVLTGLSAMVAESATFPIDLTKTRLQLHGE-SSSSSRSTNAFRLASAIVKDQGPFALY 68
           T+++L  LSAMVAES TFPIDLTKTR+QLHG  S+S +    AF + S I + +G   LY
Sbjct: 14  TRILLASLSAMVAESVTFPIDLTKTRMQLHGSGSASGAHRIGAFGVVSEIARKEGVIGLY 73

Query: 69  KGLSPAILRHLFYTPIRIVGYEHLRSLFLASD---GGSVSFHSKALVGGISGSIAQVVAS 128
           KGLSPAI+RHLFYTPIRI+GYE+L+ L + S+     S+   +KALVGG SG IAQVVAS
Sbjct: 74  KGLSPAIIRHLFYTPIRIIGYENLKGLIVRSETNNSESLPLATKALVGGFSGVIAQVVAS 133

Query: 129 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 188
           PADLVKVRMQADGRL+SQGL+PRYSGP +A TKI++ EGV GLWKGV+PN+QRAFLVNMG
Sbjct: 134 PADLVKVRMQADGRLVSQGLKPRYSGPIEAFTKILQSEGVKGLWKGVLPNIQRAFLVNMG 193

Query: 189 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 248
           ELACYDHAK FVI  ++A DNIF HT AS++SGL +T+LSCPADVVKTRMMNQ  +    
Sbjct: 194 ELACYDHAKHFVIDKKIAEDNIFAHTLASIMSGLASTSLSCPADVVKTRMMNQGEN---- 253

Query: 249 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 301
             Y +SYDCLVKTVK EG+RALWKGFFPTWARLGPWQFVFWVSYEKFR LAG+SSF
Sbjct: 254 AVYRNSYDCLVKTVKFEGIRALWKGFFPTWARLGPWQFVFWVSYEKFRLLAGISSF 305

BLAST of Chy10G182170 vs. ExPASy Swiss-Prot
Match: O95847 (Mitochondrial uncoupling protein 4 OS=Homo sapiens OX=9606 GN=SLC25A27 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.5e-65
Identity = 133/303 (43.89%), Postives = 193/303 (63.70%), Query Frame = 0

Query: 9   TKLVLTGLSAMVAESATFPIDLTKTRLQLHGESS---------SSSRSTNAFRLASAIVK 68
           +K +L+G +A VAE ATFP+DLTKTRLQ+ GE++          S+      R A  I++
Sbjct: 21  SKFLLSGCAATVAELATFPLDLTKTRLQMQGEAALARLGDGARESAPYRGMVRTALGIIE 80

Query: 69  DQGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGG-ISGSI 128
           ++G   L++G++PAI RH+ Y+  R+V YEHLR +             K+++GG ++G I
Sbjct: 81  EEGFLKLWQGVTPAIYRHVVYSGGRMVTYEHLREVVFGKSEDEHYPLWKSVIGGMMAGVI 140

Query: 129 AQVVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRA 188
            Q +A+P DLVKV+MQ +G+   +G   R+ G   A  KI+   G+ GLW G VPN+QRA
Sbjct: 141 GQFLANPTDLVKVQMQMEGKRKLEGKPLRFRGVHHAFAKILAEGGIRGLWAGWVPNIQRA 200

Query: 189 FLVNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQA 248
            LVNMG+L  YD  K +++ N    DNI  H  +S+ SGL A+ L  PADV+K+R+MNQ 
Sbjct: 201 ALVNMGDLTTYDTVKHYLVLNTPLEDNIMTHGLSSLCSGLVASILGTPADVIKSRIMNQP 260

Query: 249 ASKEGI-TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGL 301
             K+G    Y SS DCL++ V+ EG  +L+KGF P+W R+ PW  VFW++YEK R+++G+
Sbjct: 261 RDKQGRGLLYKSSTDCLIQAVQGEGFMSLYKGFLPSWLRMTPWSMVFWLTYEKIREMSGV 320

BLAST of Chy10G182170 vs. ExPASy Swiss-Prot
Match: B0G143 (Mitochondrial substrate carrier family protein ucpB OS=Dictyostelium discoideum OX=44689 GN=ucpB PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.8e-53
Identity = 113/290 (38.97%), Postives = 172/290 (59.31%), Query Frame = 0

Query: 10  KLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPFALYKG 69
           K +  GLS M A   + P+D+ KTR Q+HGE    S+S         I+K++G  A+YKG
Sbjct: 10  KFLFGGLSCMGAAVVSNPVDVLKTRFQIHGE-GIDSKSLGLVNGTIKIIKNEGISAMYKG 69

Query: 70  LSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVASPADLV 129
           L+P++LR   Y+ +R+ GY+ +++ F+ S+ G  +  SK   G +SG++   + SP DL+
Sbjct: 70  LTPSLLREATYSTLRMGGYDVIKNYFIDSN-GKTNLLSKVTSGALSGALGACITSPTDLI 129

Query: 130 KVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMGELACY 189
           KVRMQA     S+G+  +Y     A  +I+  EG+ GLWKGV P  QRA L+   ++  Y
Sbjct: 130 KVRMQAS----SKGV--KYDSISSAFKEIIAKEGIKGLWKGVGPTTQRAALLTASQIPSY 189

Query: 190 DHAKRFVIQNQLAG-DNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI-TKY 249
           DH K  ++ + +   D +  H  +S+ +GL A+  + P D+VKTR+MNQ     G+   Y
Sbjct: 190 DHIKHMILDHGIIQVDGLQVHIVSSIFAGLIASITTSPVDLVKTRIMNQPFDSNGVGLIY 249

Query: 250 NSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGL 298
            SSYDC  KT + EG+  L+KGF P W R+GP   V ++ YE  RK++G+
Sbjct: 250 KSSYDCFKKTFQSEGISGLYKGFLPNWFRIGPHTIVTFILYEYLRKVSGI 291

BLAST of Chy10G182170 vs. ExPASy Swiss-Prot
Match: Q5XGI1 (Kidney mitochondrial carrier protein 1 OS=Xenopus tropicalis OX=8364 GN=slc25a30 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.2e-51
Identity = 116/285 (40.70%), Postives = 168/285 (58.95%), Query Frame = 0

Query: 15  GLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFR-LASAIV---KDQGPFALYKGL 74
           GL+++ AE  TFPIDLTKTRLQ+ G+++ +      +R +  AIV   K++G  ALY G+
Sbjct: 13  GLASITAECGTFPIDLTKTRLQVQGQANDAKYKEIRYRGMLHAIVRIWKEEGVKALYSGI 72

Query: 75  SPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVASPADLVK 134
           +PA+LR   Y  I+I  Y+ L+ LF+       +       G +SG ++  +A+P D++K
Sbjct: 73  APAMLRQASYGTIKIGTYQSLKRLFVDCPEDE-TLVINVFCGVLSGVVSSCIANPTDVLK 132

Query: 135 VRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMGELACYD 194
           +RMQA G LI  G+   +         I + EG  GLWKGV    QRA +V   EL  YD
Sbjct: 133 IRMQAQGSLIQGGMIGNF-------INIYQQEGTRGLWKGVSLTAQRAAIVVGVELPVYD 192

Query: 195 HAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKE-GITKYNS 254
             K+ +I + L GD ++ H  AS   GL     S P DVV+TRMMNQ + +    + Y  
Sbjct: 193 ITKKHLILSGLMGDTVYTHFLASFTCGLAGALASNPVDVVRTRMMNQRSIRNVSNSSYKG 252

Query: 255 SYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 295
           + DCL++T K EG  AL+KGF+P W RLGPW  +F+++YE+ +KL
Sbjct: 253 TLDCLLQTWKNEGFFALYKGFWPNWLRLGPWNIIFFITYEQLKKL 289

BLAST of Chy10G182170 vs. ExPASy Swiss-Prot
Match: Q5SVS4 (Kidney mitochondrial carrier protein 1 OS=Homo sapiens OX=9606 GN=SLC25A30 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.1e-50
Identity = 115/288 (39.93%), Postives = 169/288 (58.68%), Query Frame = 0

Query: 12  VLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFR-LASAIV---KDQGPFALY 71
           V  GL+++ AE  TFPIDLTKTRLQ+ G+++ +      +R +  A+V   +++G  ALY
Sbjct: 10  VYGGLASITAECGTFPIDLTKTRLQIQGQTNDAKFKEIRYRGMLHALVRIGREEGLKALY 69

Query: 72  KGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVASPAD 131
            G++PA+LR   Y  I+I  Y+ L+ LF+       +     + G +SG I+  +A+P D
Sbjct: 70  SGIAPAMLRQASYGTIKIGTYQSLKRLFIERPEDE-TLPINVICGILSGVISSTIANPTD 129

Query: 132 LVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMGELA 191
           ++K+RMQA    I  G+   +         I + EG  GLWKGV    QRA +V   EL 
Sbjct: 130 VLKIRMQAQSNTIQGGMIGNF-------MNIYQQEGTRGLWKGVSLTAQRAAIVVGVELP 189

Query: 192 CYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEG-ITK 251
            YD  K+ +I + L GD ++ H  +S   GL     S P DVV+TRMMNQ   ++G  + 
Sbjct: 190 VYDITKKHLILSGLMGDTVYTHFLSSFTCGLAGALASNPVDVVRTRMMNQRVLRDGRCSG 249

Query: 252 YNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 295
           Y  + DCL++T K EG  AL+KGF+P W RLGPW  +F+V+YE+ +KL
Sbjct: 250 YTGTLDCLLQTWKNEGFFALYKGFWPNWLRLGPWNIIFFVTYEQLKKL 289

BLAST of Chy10G182170 vs. ExPASy TrEMBL
Match: A0A5A7SUW0 (Mitochondrial uncoupling protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00220 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.6e-166
Identity = 297/300 (99.00%), Postives = 299/300 (99.67%), Query Frame = 0

Query: 1   MEATHNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60
           MEATHNPYTKL+LTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD
Sbjct: 1   MEATHNPYTKLLLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60

Query: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQ 120
           QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASD GSVSFHSKALVGGISGSIAQ
Sbjct: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDAGSVSFHSKALVGGISGSIAQ 120

Query: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180
           VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL
Sbjct: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180

Query: 181 VNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240
           VNMGELACYDHAKRFVIQNQ+AGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS
Sbjct: 181 VNMGELACYDHAKRFVIQNQIAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240

Query: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300
           KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF
Sbjct: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300

BLAST of Chy10G182170 vs. ExPASy TrEMBL
Match: A0A1S3BFM4 (mitochondrial uncoupling protein 3 OS=Cucumis melo OX=3656 GN=LOC103489145 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.6e-166
Identity = 297/300 (99.00%), Postives = 299/300 (99.67%), Query Frame = 0

Query: 1   MEATHNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60
           MEATHNPYTKL+LTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD
Sbjct: 1   MEATHNPYTKLLLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60

Query: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQ 120
           QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASD GSVSFHSKALVGGISGSIAQ
Sbjct: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDAGSVSFHSKALVGGISGSIAQ 120

Query: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180
           VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL
Sbjct: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180

Query: 181 VNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240
           VNMGELACYDHAKRFVIQNQ+AGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS
Sbjct: 181 VNMGELACYDHAKRFVIQNQIAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240

Query: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300
           KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF
Sbjct: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300

BLAST of Chy10G182170 vs. ExPASy TrEMBL
Match: A0A6J1GZH9 (mitochondrial uncoupling protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111458911 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 3.8e-152
Identity = 270/296 (91.22%), Postives = 283/296 (95.61%), Query Frame = 0

Query: 5   HNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64
           HNP TK++LT LSAMVAES TFPIDLTKTRLQLHGESSSSSRSTNAFR+ASAIVKDQGPF
Sbjct: 10  HNPNTKMLLTALSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRVASAIVKDQGPF 69

Query: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVAS 124
            LYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVS  +KALVGGISGSIAQVVAS
Sbjct: 70  GLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLPAKALVGGISGSIAQVVAS 129

Query: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184
           PADLVKVRMQADGRL+SQGLQPRYSGP DA TKIVRGEG++GLWKGVVPNVQRAFLVNMG
Sbjct: 130 PADLVKVRMQADGRLMSQGLQPRYSGPLDAFTKIVRGEGIMGLWKGVVPNVQRAFLVNMG 189

Query: 185 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244
           ELACYDHAKRFVI+NQ+AGDNIFGHT ASV+SGL ATALSCPADVVKTRMMNQAASKEG 
Sbjct: 190 ELACYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMMNQAASKEGT 249

Query: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 301
           T YN +YDCLVKTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAG+SSF
Sbjct: 250 TMYNGAYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGISSF 305

BLAST of Chy10G182170 vs. ExPASy TrEMBL
Match: A0A6J1KAM5 (mitochondrial uncoupling protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491606 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.2e-149
Identity = 265/296 (89.53%), Postives = 280/296 (94.59%), Query Frame = 0

Query: 5   HNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64
           HNP TK++LT LSAMVAES TFPIDLTKTRLQL GESSS S STNAFR+ASAIVKDQGPF
Sbjct: 10  HNPNTKMLLTALSAMVAESTTFPIDLTKTRLQLQGESSSFSSSTNAFRVASAIVKDQGPF 69

Query: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVAS 124
            LYKGLSPAILRHLFYTPIRIVGYEHL+SLFL SDGGSVS  +KALVGGISGSIAQVVAS
Sbjct: 70  GLYKGLSPAILRHLFYTPIRIVGYEHLQSLFLVSDGGSVSLPAKALVGGISGSIAQVVAS 129

Query: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184
           PADLVKVRMQADGRL+S+GLQPRYSGPFDALTKIVRGEG++GLWKGVVPNVQRAFLVNMG
Sbjct: 130 PADLVKVRMQADGRLMSRGLQPRYSGPFDALTKIVRGEGIMGLWKGVVPNVQRAFLVNMG 189

Query: 185 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244
           EL CYDHAKRFVI+NQ+AGDNIFGHT ASV+SGL ATALSCPADVVKTRMMNQAASKEG 
Sbjct: 190 ELPCYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMMNQAASKEGT 249

Query: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 301
           T YN +YDCLVKTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAG+SSF
Sbjct: 250 TMYNGTYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGISSF 305

BLAST of Chy10G182170 vs. ExPASy TrEMBL
Match: A0A6J1DAE7 (mitochondrial uncoupling protein 3 OS=Momordica charantia OX=3673 GN=LOC111019146 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.8e-148
Identity = 266/297 (89.56%), Postives = 279/297 (93.94%), Query Frame = 0

Query: 5   HNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64
           HN YTKL LT LSAMVAE+ TFP+DL KTRLQLHGE  SSSRSTNAFR+AS+IVK+QGPF
Sbjct: 10  HNSYTKLFLTSLSAMVAETTTFPMDLIKTRLQLHGE--SSSRSTNAFRVASSIVKEQGPF 69

Query: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVAS 124
            LY+GLSPAILRHLFYTPIRIVGYEHLRSLFLA D GS+S H+KALVGGISGSIAQVVAS
Sbjct: 70  GLYRGLSPAILRHLFYTPIRIVGYEHLRSLFLAPDSGSLSLHAKALVGGISGSIAQVVAS 129

Query: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184
           PADLVKVRMQADGRL SQGLQPRYSGPFDALTKIVR EG++GLWKGVVPNVQRAFLVNMG
Sbjct: 130 PADLVKVRMQADGRLRSQGLQPRYSGPFDALTKIVRAEGIIGLWKGVVPNVQRAFLVNMG 189

Query: 185 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQ-AASKEG 244
           ELACYDHAKRF+I+NQ+AGDNIFGHT ASVISGLCAT LSCPADVVKTRMMNQ AASKEG
Sbjct: 190 ELACYDHAKRFIIENQIAGDNIFGHTFASVISGLCATTLSCPADVVKTRMMNQAAASKEG 249

Query: 245 ITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 301
             KYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF
Sbjct: 250 TIKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 304

BLAST of Chy10G182170 vs. NCBI nr
Match: XP_004135167.1 (mitochondrial uncoupling protein 3 [Cucumis sativus] >KAE8648752.1 hypothetical protein Csa_009240 [Cucumis sativus])

HSP 1 Score: 598 bits (1543), Expect = 9.18e-216
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MEATHNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60
           MEATHNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD
Sbjct: 1   MEATHNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60

Query: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQ 120
           QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQ
Sbjct: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQ 120

Query: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180
           VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL
Sbjct: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180

Query: 181 VNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240
           VNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS
Sbjct: 181 VNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240

Query: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300
           KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF
Sbjct: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300

BLAST of Chy10G182170 vs. NCBI nr
Match: XP_008446389.1 (PREDICTED: mitochondrial uncoupling protein 3 [Cucumis melo] >KAA0034438.1 mitochondrial uncoupling protein 3 [Cucumis melo var. makuwa] >TYK17696.1 mitochondrial uncoupling protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 594 bits (1532), Expect = 4.36e-214
Identity = 297/300 (99.00%), Postives = 299/300 (99.67%), Query Frame = 0

Query: 1   MEATHNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60
           MEATHNPYTKL+LTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD
Sbjct: 1   MEATHNPYTKLLLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKD 60

Query: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQ 120
           QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASD GSVSFHSKALVGGISGSIAQ
Sbjct: 61  QGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDAGSVSFHSKALVGGISGSIAQ 120

Query: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180
           VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL
Sbjct: 121 VVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFL 180

Query: 181 VNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240
           VNMGELACYDHAKRFVIQNQ+AGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS
Sbjct: 181 VNMGELACYDHAKRFVIQNQIAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAAS 240

Query: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300
           KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF
Sbjct: 241 KEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300

BLAST of Chy10G182170 vs. NCBI nr
Match: XP_038892574.1 (mitochondrial uncoupling protein 3 [Benincasa hispida])

HSP 1 Score: 559 bits (1441), Expect = 3.90e-200
Identity = 277/296 (93.58%), Postives = 284/296 (95.95%), Query Frame = 0

Query: 5   HNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64
           +NPY KL LT LSAMVAES TFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF
Sbjct: 10  YNPYAKLFLTALSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 69

Query: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVAS 124
           ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSV  H KALVGG+SGSIAQVVAS
Sbjct: 70  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVPLHVKALVGGLSGSIAQVVAS 129

Query: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184
           PADLVKVRMQADGRL+SQGLQPRYSGP DALTKIV GEG++GLWKGVVPNVQRAFLVNMG
Sbjct: 130 PADLVKVRMQADGRLVSQGLQPRYSGPLDALTKIVHGEGIIGLWKGVVPNVQRAFLVNMG 189

Query: 185 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244
           ELACYDHAKRFV+QNQ+AGDNIFGHT ASVISGLCATALSCPADVVKTRMMNQA SKEGI
Sbjct: 190 ELACYDHAKRFVLQNQIAGDNIFGHTFASVISGLCATALSCPADVVKTRMMNQAVSKEGI 249

Query: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300
            KYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF
Sbjct: 250 AKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 305

BLAST of Chy10G182170 vs. NCBI nr
Match: XP_022957541.1 (mitochondrial uncoupling protein 3 [Cucurbita moschata])

HSP 1 Score: 546 bits (1407), Expect = 5.92e-195
Identity = 270/296 (91.22%), Postives = 283/296 (95.61%), Query Frame = 0

Query: 5   HNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64
           HNP TK++LT LSAMVAES TFPIDLTKTRLQLHGESSSSSRSTNAFR+ASAIVKDQGPF
Sbjct: 10  HNPNTKMLLTALSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRVASAIVKDQGPF 69

Query: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVAS 124
            LYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVS  +KALVGGISGSIAQVVAS
Sbjct: 70  GLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLPAKALVGGISGSIAQVVAS 129

Query: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184
           PADLVKVRMQADGRL+SQGLQPRYSGP DA TKIVRGEG++GLWKGVVPNVQRAFLVNMG
Sbjct: 130 PADLVKVRMQADGRLMSQGLQPRYSGPLDAFTKIVRGEGIMGLWKGVVPNVQRAFLVNMG 189

Query: 185 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244
           ELACYDHAKRFVI+NQ+AGDNIFGHT ASV+SGL ATALSCPADVVKTRMMNQAASKEG 
Sbjct: 190 ELACYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMMNQAASKEGT 249

Query: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300
           T YN +YDCLVKTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAG+SSF
Sbjct: 250 TMYNGAYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGISSF 305

BLAST of Chy10G182170 vs. NCBI nr
Match: KAG7032229.1 (Mitochondrial uncoupling protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 545 bits (1404), Expect = 1.70e-194
Identity = 269/296 (90.88%), Postives = 283/296 (95.61%), Query Frame = 0

Query: 5   HNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64
           HNP TK++LT L+AMVAES TFPIDLTKTRLQLHGESSSSSRSTNAFR+ASAIVKDQGPF
Sbjct: 10  HNPNTKMLLTALAAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRVASAIVKDQGPF 69

Query: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVAS 124
            LYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVS  +KALVGGISGSIAQVVAS
Sbjct: 70  GLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLPAKALVGGISGSIAQVVAS 129

Query: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184
           PADLVKVRMQADGRL+SQGLQPRYSGP DA TKIVRGEG++GLWKGVVPNVQRAFLVNMG
Sbjct: 130 PADLVKVRMQADGRLMSQGLQPRYSGPLDAFTKIVRGEGIMGLWKGVVPNVQRAFLVNMG 189

Query: 185 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244
           ELACYDHAKRFVI+NQ+AGDNIFGHT ASV+SGL ATALSCPADVVKTRMMNQAASKEG 
Sbjct: 190 ELACYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMMNQAASKEGT 249

Query: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300
           T YN +YDCLVKTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAG+SSF
Sbjct: 250 TMYNGTYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGISSF 305

BLAST of Chy10G182170 vs. TAIR 10
Match: AT1G14140.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 419.1 bits (1076), Expect = 3.0e-117
Identity = 209/296 (70.61%), Postives = 243/296 (82.09%), Query Frame = 0

Query: 9   TKLVLTGLSAMVAESATFPIDLTKTRLQLHGE-SSSSSRSTNAFRLASAIVKDQGPFALY 68
           T+++L  LSAMVAES TFPIDLTKTR+QLHG  S+S +    AF + S I + +G   LY
Sbjct: 14  TRILLASLSAMVAESVTFPIDLTKTRMQLHGSGSASGAHRIGAFGVVSEIARKEGVIGLY 73

Query: 69  KGLSPAILRHLFYTPIRIVGYEHLRSLFLASD---GGSVSFHSKALVGGISGSIAQVVAS 128
           KGLSPAI+RHLFYTPIRI+GYE+L+ L + S+     S+   +KALVGG SG IAQVVAS
Sbjct: 74  KGLSPAIIRHLFYTPIRIIGYENLKGLIVRSETNNSESLPLATKALVGGFSGVIAQVVAS 133

Query: 129 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 188
           PADLVKVRMQADGRL+SQGL+PRYSGP +A TKI++ EGV GLWKGV+PN+QRAFLVNMG
Sbjct: 134 PADLVKVRMQADGRLVSQGLKPRYSGPIEAFTKILQSEGVKGLWKGVLPNIQRAFLVNMG 193

Query: 189 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 248
           ELACYDHAK FVI  ++A DNIF HT AS++SGL +T+LSCPADVVKTRMMNQ  +    
Sbjct: 194 ELACYDHAKHFVIDKKIAEDNIFAHTLASIMSGLASTSLSCPADVVKTRMMNQGEN---- 253

Query: 249 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 301
             Y +SYDCLVKTVK EG+RALWKGFFPTWARLGPWQFVFWVSYEKFR LAG+SSF
Sbjct: 254 AVYRNSYDCLVKTVKFEGIRALWKGFFPTWARLGPWQFVFWVSYEKFRLLAGISSF 305

BLAST of Chy10G182170 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 194.1 bits (492), Expect = 1.6e-49
Identity = 112/306 (36.60%), Postives = 165/306 (53.92%), Query Frame = 0

Query: 15  GLSAMVAESATFPIDLTKTRLQLHGESSSSSRST-------------------------- 74
           G+++++A  +T P+DL K RLQLHGE+ S++  T                          
Sbjct: 10  GIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNSSPAAFLETTSSVPKV 69

Query: 75  NAFRLASAIVKDQGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSK 134
               L   IVK +G  AL+ G+S  +LR   Y+  R+  YE L++ +   + G ++   K
Sbjct: 70  GPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKWTDPESGKLNLSRK 129

Query: 135 ALVGGISGSIAQVVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLW 194
              G ++G I   V +PAD+  VRMQADGRL     +  Y+G  DA+  +V+GEGV  LW
Sbjct: 130 IGAGLVAGGIGAAVGNPADVAMVRMQADGRL-PLAQRRNYAGVGDAIRSMVKGEGVTSLW 189

Query: 195 KGVVPNVQRAFLVNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPAD 254
           +G    + RA +V   +LA YD  K  +++N +  D +  H  AS  +G  A+  S P D
Sbjct: 190 RGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAAGFVASVASNPVD 249

Query: 255 VVKTRMMNQAASKEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSY 295
           V+KTR+MN       +  Y+ ++DC VKTVK EG  AL+KGF PT  R GP+  V +V+ 
Sbjct: 250 VIKTRVMNMK-----VGAYDGAWDCAVKTVKAEGAMALYKGFVPTVCRQGPFTVVLFVTL 309

BLAST of Chy10G182170 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 185.3 bits (469), Expect = 7.4e-47
Identity = 105/299 (35.12%), Postives = 164/299 (54.85%), Query Frame = 0

Query: 15  GLSAMVAESATFPIDLTKTRLQLHGESS------------SSSRSTNA-------FRLAS 74
           G++++VA  +T P+DL K R+QL GES+             +S + NA         + S
Sbjct: 10  GIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAPPLRVGVIGVGS 69

Query: 75  AIVKDQGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGIS 134
            +++++G  AL+ G+S  +LR   Y+  R+  Y+ ++  +   +  ++    K   G I+
Sbjct: 70  RLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPETKTMPLMKKIGAGAIA 129

Query: 135 GSIAQVVASPADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNV 194
           G+I   V +PAD+  VRMQADGRL     +  Y    DA+T+++RGEGV  LW+G    +
Sbjct: 130 GAIGAAVGNPADVAMVRMQADGRLPLTD-RRNYKSVLDAITQMIRGEGVTSLWRGSSLTI 189

Query: 195 QRAFLVNMGELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMM 254
            RA LV   +LA YD  K  +++  L  D +  H  AS  +G  A+  S P DV+KTR+M
Sbjct: 190 NRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGFVASVASNPVDVIKTRVM 249

Query: 255 NQAASKEGITKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 295
           N          Y  + DC +KTVK EG+ +L+KGF PT +R  P+  V +V+ E+ +KL
Sbjct: 250 NMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVTLEQVKKL 307

BLAST of Chy10G182170 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 185.3 bits (469), Expect = 7.4e-47
Identity = 102/289 (35.29%), Postives = 163/289 (56.40%), Query Frame = 0

Query: 12  VLTGLSAMVAESATFPIDLTKTRLQLH-----GESSSSSRSTNAFRLASAIVKDQGPFAL 71
           + +  +A  AE  T P+D  K RLQL      G+  +  +   +    + I +++G   L
Sbjct: 17  ICSAFAACFAELCTIPLDTAKVRLQLQRKIPTGDGENLPKYRGSIGTLATIAREEGISGL 76

Query: 72  YKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDG-GSVSFHSKALVGGISGSIAQVVASP 131
           +KG+   + R   Y  +RI  YE +++L + SD  G +  + K L   ++G+IA +VA+P
Sbjct: 77  WKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDFIGDIPLYQKILAALLTGAIAIIVANP 136

Query: 132 ADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMGE 191
            DLVKVR+Q++G+L   G+  RY+G  DA   IV+ EGV  LW G+ PN+ R  +VN  E
Sbjct: 137 TDLVKVRLQSEGKL-PAGVPRRYAGAVDAYFTIVKLEGVSALWTGLGPNIARNAIVNAAE 196

Query: 192 LACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGIT 251
           LA YD  K  +++     D++  H  A + +G  A  +  P DVVK+RMM       G +
Sbjct: 197 LASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFAVCIGSPIDVVKSRMM-------GDS 256

Query: 252 KYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 295
            Y ++ DC +KT+K EG+ A +KGF P + RLG W  + +++ E+ +K+
Sbjct: 257 TYRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGTWNAIMFLTLEQVKKV 297

BLAST of Chy10G182170 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 180.6 bits (457), Expect = 1.8e-45
Identity = 102/285 (35.79%), Postives = 159/285 (55.79%), Query Frame = 0

Query: 14  TGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFR----LASAIVKDQGPFALYKG 73
           +  +A V E  T P+D  K RLQL   + +   +   +R        I +++G  +L+KG
Sbjct: 18  SAFAACVGEVCTIPLDTAKVRLQLQKSALAGDVTLPKYRGLLGTVGTIAREEGLRSLWKG 77

Query: 74  LSPAILRHLFYTPIRIVGYEHLRSLFLASD-GGSVSFHSKALVGGISGSIAQVVASPADL 133
           + P + R   +  +RI  YE +++L++  D  G V    K L G  +G++  +VA+P DL
Sbjct: 78  VVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPLSKKILAGLTTGALGIMVANPTDL 137

Query: 134 VKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMGELAC 193
           VKVR+QA+G+L + G   RYSG  +A + IVR EGV  LW G+ PNV R  ++N  ELA 
Sbjct: 138 VKVRLQAEGKL-AAGAPRRYSGALNAYSTIVRQEGVRALWTGLGPNVARNAIINAAELAS 197

Query: 194 YDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGITKYN 253
           YD  K  +++     DN+  H  + + +G  A  +  P DVVK+RMM  + +      Y 
Sbjct: 198 YDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSPVDVVKSRMMGDSGA------YK 257

Query: 254 SSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRK 294
            + DC VKT+K +G  A +KGF P + RLG W  + +++ E+ +K
Sbjct: 258 GTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQAKK 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XI744.3e-11670.61Mitochondrial uncoupling protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUMP3 PE=2... [more]
O958471.5e-6543.89Mitochondrial uncoupling protein 4 OS=Homo sapiens OX=9606 GN=SLC25A27 PE=2 SV=1[more]
B0G1438.8e-5338.97Mitochondrial substrate carrier family protein ucpB OS=Dictyostelium discoideum ... [more]
Q5XGI18.2e-5140.70Kidney mitochondrial carrier protein 1 OS=Xenopus tropicalis OX=8364 GN=slc25a30... [more]
Q5SVS44.1e-5039.93Kidney mitochondrial carrier protein 1 OS=Homo sapiens OX=9606 GN=SLC25A30 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A5A7SUW01.6e-16699.00Mitochondrial uncoupling protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BFM41.6e-16699.00mitochondrial uncoupling protein 3 OS=Cucumis melo OX=3656 GN=LOC103489145 PE=3 ... [more]
A0A6J1GZH93.8e-15291.22mitochondrial uncoupling protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111458911... [more]
A0A6J1KAM55.2e-14989.53mitochondrial uncoupling protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491606 P... [more]
A0A6J1DAE79.8e-14889.56mitochondrial uncoupling protein 3 OS=Momordica charantia OX=3673 GN=LOC11101914... [more]
Match NameE-valueIdentityDescription
XP_004135167.19.18e-216100.00mitochondrial uncoupling protein 3 [Cucumis sativus] >KAE8648752.1 hypothetical ... [more]
XP_008446389.14.36e-21499.00PREDICTED: mitochondrial uncoupling protein 3 [Cucumis melo] >KAA0034438.1 mitoc... [more]
XP_038892574.13.90e-20093.58mitochondrial uncoupling protein 3 [Benincasa hispida][more]
XP_022957541.15.92e-19591.22mitochondrial uncoupling protein 3 [Cucurbita moschata][more]
KAG7032229.11.70e-19490.88Mitochondrial uncoupling protein 3, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
AT1G14140.13.0e-11770.61Mitochondrial substrate carrier family protein [more]
AT4G24570.11.6e-4936.60dicarboxylate carrier 2 [more]
AT2G22500.17.4e-4735.12uncoupling protein 5 [more]
AT5G58970.17.4e-4735.29uncoupling protein 2 [more]
AT3G54110.11.8e-4535.79plant uncoupling mitochondrial protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002067Mitochondrial carrier proteinPRINTSPR00926MITOCARRIERcoord: 23..37
score: 35.3
coord: 213..235
score: 33.14
coord: 10..23
score: 23.07
coord: 118..136
score: 23.12
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 205..295
e-value: 4.0E-21
score: 74.7
coord: 6..96
e-value: 1.6E-19
score: 69.6
coord: 103..199
e-value: 6.7E-21
score: 74.0
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 204..294
score: 26.031319
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 103..195
score: 27.232693
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 9..94
score: 22.448652
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 9..296
e-value: 4.3E-84
score: 283.8
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 5..290
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 4..292
NoneNo IPR availablePANTHERPTHR45618:SF8MITOCHONDRIAL UNCOUPLING PROTEIN 4coord: 4..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G182170.1Chy10G182170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006839 mitochondrial transport
biological_process GO:0009409 response to cold
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031966 mitochondrial membrane