Chy10G181720 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G181720
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pectate lyase
Location	chrH10: 15262669 .. 15264208 (+)
RNA-Seq Expression	Chy10G181720
Synteny	Chy10G181720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGCACTTAGCCTGCTGATAACATTGTCCTTGCTGGCTACTACTGCATTTGCTGACATTGGAGACTTCGATGACTACTGGAAAGCACGTGCTGCCGCCGCCGAAAAAGCTGCTGCTGACGCCTATGAACCTGATCCTGAGACCGTCGCCAACACCGTCAATGCTGAAATTGCCCAGTAAGTTCGAAATTTTTAGCAACATAATTCTAGTATAAAGAATCATTACTAAACTCAGAACTGAGTTTGACGCAGCGCATTGAGTGGAAGGAATAGTACAAGGAGGAATTTGAAGAAATATGCAGGGCCATGTCTAGCCACTAATCCGATCGATCGGTGCTGGAGATGTGACCCCAATTGGGCCCAGAACAGGAAGAAGCTAGCCGATTGTGTTCTGGGTTTTGGCCGCAAGACCACTGGTGGAAAGTTGGGTCCATACTATGTCGTTAACGACTCGTCGGATTCGGACCTCATGAACCCAAAACCTGGGACTCTTCGTCATGCTGTGATCCAAAAGGGACCTCTGTGGATCATCTTCTCTACCAACATGGCCATTAGGCTCTCTCAGGAGCTCATCATGACTAGCGACAAGACCATTGATGCAAGAGGTGCTAATGTTCAGATTGCTTATGGTGCTGGCATTACTCTTCAGTACATAAGAAATGTCATCATCCATGGTCTCCGTATCCATCACATTGTTGTTGGTAGCGGTGGAATGATCAGAGACGCTGTCGACCATGTGGGTTTGAGGACTATGAGTGACGGCGATGGCATTTCCATCTTCGGCTCCTCCAATGTTTGGATTGACCATGTGTCCATGTCCAATTGTCATGATGGTCTCATTGATGCTATTATGGGATCTACTGCCATCACTATATCCAATTGTCATTTCACTCACCACAATGAGGTAATAATGTTTCTTGCCCTGAAACTATTTGCCTGACGGTTTTATATAATTGAAGTTAAAAGCTAAGTTACATACAGGTGATGTTGTTTGGAGCGAGTGACGGATACTCACAAGATCAGATAATGCAAGTGACAGTAGCATTCAACCACTTTGGTCAAGGATTGGTGCAGCGAATGCCAAGATGTCGATGGGGCTTCTTCCATGTTGTCAACAATGATTACACTCATTGGCTTATGTATGCCATCGGTGGAAGCCAGCATCCCACCATTGTCAGCCAGGGCAATCGTTTCATTGCTCCTCCCAATGTATATGCTAAAGAGGTAATCAAATATTTAACAAACAGCTCAAGCAATTCATTTGTTATTAGTTTTGTCAAGAAACTAATAGTGGGTGTGCACTTAAAACATAGGTGACAAAGAGAGAGTACTCACCAGAGCAAGTTTGGAAAAGCTGGACATGGAGATCAGAGGGAGACTTGATGATGAATGGAGCATTCTTTGTTACCTCAGGTGATCAAAGCAAAAGAAGACCCTTCTCAAGGATGGATATGATCTCCTATAAACCAGGGACATACGTTAGAAGAATGACACGTTTTGCAGGCTCGCTCGGCTGCTTCGTTGGTCGCCCTTGCTAA

mRNA sequence

ATGCCTGCACTTAGCCTGCTGATAACATTGTCCTTGCTGGCTACTACTGCATTTGCTGACATTGGAGACTTCGATGACTACTGGAAAGCACGTGCTGCCGCCGCCGAAAAAGCTGCTGCTGACGCCTATGAACCTGATCCTGAGACCGTCGCCAACACCGTCAATGCTGAAATTGCCCACGCATTGAGTGGAAGGAATAGTACAAGGAGGAATTTGAAGAAATATGCAGGGCCATGTCTAGCCACTAATCCGATCGATCGGTGCTGGAGATGTGACCCCAATTGGGCCCAGAACAGGAAGAAGCTAGCCGATTGTGTTCTGGGTTTTGGCCGCAAGACCACTGGTGGAAAGTTGGGTCCATACTATGTCGTTAACGACTCGTCGGATTCGGACCTCATGAACCCAAAACCTGGGACTCTTCGTCATGCTGTGATCCAAAAGGGACCTCTGTGGATCATCTTCTCTACCAACATGGCCATTAGGCTCTCTCAGGAGCTCATCATGACTAGCGACAAGACCATTGATGCAAGAGGTGCTAATGTTCAGATTGCTTATGGTGCTGGCATTACTCTTCAGTACATAAGAAATGTCATCATCCATGGTCTCCGTATCCATCACATTGTTGTTGGTAGCGGTGGAATGATCAGAGACGCTGTCGACCATGTGGGTTTGAGGACTATGAGTGACGGCGATGGCATTTCCATCTTCGGCTCCTCCAATGTTTGGATTGACCATGTGTCCATGTCCAATTGTCATGATGGTCTCATTGATGCTATTATGGGATCTACTGCCATCACTATATCCAATTGTCATTTCACTCACCACAATGAGGTGATGTTGTTTGGAGCGAGTGACGGATACTCACAAGATCAGATAATGCAAGTGACAGTAGCATTCAACCACTTTGGTCAAGGATTGGTGCAGCGAATGCCAAGATGTCGATGGGGCTTCTTCCATGTTGTCAACAATGATTACACTCATTGGCTTATGTATGCCATCGGTGGAAGCCAGCATCCCACCATTGTCAGCCAGGGCAATCGTTTCATTGCTCCTCCCAATGTATATGCTAAAGAGGTGACAAAGAGAGAGTACTCACCAGAGCAAGTTTGGAAAAGCTGGACATGGAGATCAGAGGGAGACTTGATGATGAATGGAGCATTCTTTGTTACCTCAGGTGATCAAAGCAAAAGAAGACCCTTCTCAAGGATGGATATGATCTCCTATAAACCAGGGACATACGTTAGAAGAATGACACGTTTTGCAGGCTCGCTCGGCTGCTTCGTTGGTCGCCCTTGCTAA

Coding sequence (CDS)

ATGCCTGCACTTAGCCTGCTGATAACATTGTCCTTGCTGGCTACTACTGCATTTGCTGACATTGGAGACTTCGATGACTACTGGAAAGCACGTGCTGCCGCCGCCGAAAAAGCTGCTGCTGACGCCTATGAACCTGATCCTGAGACCGTCGCCAACACCGTCAATGCTGAAATTGCCCACGCATTGAGTGGAAGGAATAGTACAAGGAGGAATTTGAAGAAATATGCAGGGCCATGTCTAGCCACTAATCCGATCGATCGGTGCTGGAGATGTGACCCCAATTGGGCCCAGAACAGGAAGAAGCTAGCCGATTGTGTTCTGGGTTTTGGCCGCAAGACCACTGGTGGAAAGTTGGGTCCATACTATGTCGTTAACGACTCGTCGGATTCGGACCTCATGAACCCAAAACCTGGGACTCTTCGTCATGCTGTGATCCAAAAGGGACCTCTGTGGATCATCTTCTCTACCAACATGGCCATTAGGCTCTCTCAGGAGCTCATCATGACTAGCGACAAGACCATTGATGCAAGAGGTGCTAATGTTCAGATTGCTTATGGTGCTGGCATTACTCTTCAGTACATAAGAAATGTCATCATCCATGGTCTCCGTATCCATCACATTGTTGTTGGTAGCGGTGGAATGATCAGAGACGCTGTCGACCATGTGGGTTTGAGGACTATGAGTGACGGCGATGGCATTTCCATCTTCGGCTCCTCCAATGTTTGGATTGACCATGTGTCCATGTCCAATTGTCATGATGGTCTCATTGATGCTATTATGGGATCTACTGCCATCACTATATCCAATTGTCATTTCACTCACCACAATGAGGTGATGTTGTTTGGAGCGAGTGACGGATACTCACAAGATCAGATAATGCAAGTGACAGTAGCATTCAACCACTTTGGTCAAGGATTGGTGCAGCGAATGCCAAGATGTCGATGGGGCTTCTTCCATGTTGTCAACAATGATTACACTCATTGGCTTATGTATGCCATCGGTGGAAGCCAGCATCCCACCATTGTCAGCCAGGGCAATCGTTTCATTGCTCCTCCCAATGTATATGCTAAAGAGGTGACAAAGAGAGAGTACTCACCAGAGCAAGTTTGGAAAAGCTGGACATGGAGATCAGAGGGAGACTTGATGATGAATGGAGCATTCTTTGTTACCTCAGGTGATCAAAGCAAAAGAAGACCCTTCTCAAGGATGGATATGATCTCCTATAAACCAGGGACATACGTTAGAAGAATGACACGTTTTGCAGGCTCGCTCGGCTGCTTCGTTGGTCGCCCTTGCTAA

Protein sequence

MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAHALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGSLGCFVGRPC*

Homology

[Close]

BLAST of Chy10G181720 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 2.9e-166
Identity = 286/442 (64.71%), Postives = 331/442 (74.89%), Query Frame = 0


Query: 4   LSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAHAL- 63
           L L IT + +  +  A IG +D+ W+ RA  A++ A + YEP PE V    N ++   + 
Sbjct: 11  LFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMK 70

Query: 64  -------SGRNSTRRNL--KKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKT 123
                  +  NSTRR L  KKY GPC+ TNPID+CWRCDPNWA NRKKLADC +GFG K 
Sbjct: 71  ELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKA 130

Query: 124 TGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKT 183
            GGK G +YVV D+SD D  +PKPGTLRHAVIQK PLWIIF   M IRL QE+IM SDKT
Sbjct: 131 IGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKT 190

Query: 184 IDARGANVQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGI 243
           IDARG NV I  GAGITLQYI+NVIIHGL IH IV G+GGM+RDAVDH+G+RT SDGDGI
Sbjct: 191 IDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGI 250

Query: 244 SIFGSSNVWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIM 303
           SIFG+S +WIDHVSM  C+DGLIDA+ GST ITISN HFT HNEVMLFGASD  S DQ+M
Sbjct: 251 SIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVM 310

Query: 304 QVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPN 363
           Q+T+AFNHFG+ L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HPTI+ QGNRFIAPP+
Sbjct: 311 QITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPD 370

Query: 364 VYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGD---QSKRRPFSRMDMISYKP 423
           ++ K+VTKREY+PE VW  WTWRSEG+L MNGA+F  SGD    SK +     D IS  P
Sbjct: 371 IFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDL--YDGISAAP 430

Query: 424 GTYVRRMTRFAGSLGCFVGRPC 433
              V  MTRFAG LGC  G+PC
Sbjct: 431 AEDVTWMTRFAGVLGCKPGKPC 449

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BLAST of Chy10G181720 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.4e-155
Identity = 263/420 (62.62%), Postives = 317/420 (75.48%), Query Frame = 0


Query: 14  ATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAHALSGRNSTRRNLK 73
           A  + A+I +FD+YW+ ++  A+  A  AY P PE V N  N  +  +  G NSTRRNL+
Sbjct: 17  AALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NSTRRNLR 76

Query: 74  -KYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYVVNDSSDSDL 133
               G CLATNPIDRCWRC  NW+ NRK L  CV GFGRKTTGG  G  YVV D SD  L
Sbjct: 77  TNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDSL 136

Query: 134 MNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQIAYGAGITLQ 193
            +PK GTLR  VIQ  PLWIIF  +M IRL QELI+ +DKTID RGANVQIA GA +T+Q
Sbjct: 137 TDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQ 196

Query: 194 YIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCH 253
           ++ NVIIHG+ IH I  G GG+IRD+  H G+RT SDGDGISI GSSN+WIDHVS++ C 
Sbjct: 197 FVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCS 256

Query: 254 DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFGQGLVQRMPR 313
           DGLID I+GSTAITISNCH T H++VML GASD Y+QD+IMQVTVAFNHFG+GLVQRMPR
Sbjct: 257 DGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPR 316

Query: 314 CRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKREYSPEQVWKS 373
           CR+GF HVVNNDYTHW+MYA+GGSQHPTI+SQGNR+IAP    AKEVTKR+Y+    W  
Sbjct: 317 CRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWSK 376

Query: 374 WTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGSLGCFVGRPC 433
           WTW+S+GDL ++GAFFV SG   + + +S+ D+I  KPGT+V+R+TRF+G+L C     C
Sbjct: 377 WTWKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNCKENMEC 434

[Close]

BLAST of Chy10G181720 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.4e-152
Identity = 263/440 (59.77%), Postives = 317/440 (72.05%), Query Frame = 0


Query: 10  LSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEI---AHALSGRN 69
           L+++A     ++  FD YW  R + A K    +Y+P P  V N  N  +     A   RN
Sbjct: 20  LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 70  STRRNL---------KKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGK 129
            TRR L          K +G CLA NPID CWRCD NWA NRKKLADCVLGFGR+TTGGK
Sbjct: 80  DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 130 LGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDAR 189
            GP YVV D+SD+DL+NPKPGTLRHAV + GPLWIIF+ +M I+L QEL++TSDKTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 190 GANVQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFG 249
           GA V I  GAG+TLQ++ NVIIH + + HIV G+GG+IRD+  H+GLRT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 250 SSNVWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTV 309
           ++N+WIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D +  D+ MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 310 AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAK 369
           AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTI+SQGNRFIAPPN  AK
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 370 EVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSG-----DQSKRRPFSRMDMISYKPGT 429
           ++TKREY+P   WKSW W+SEGD  +NGA+FV SG         + P      I  KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 430 YVRRMTRFAGSLGCFVGRPC 433
            VR++T  AG LGC +G  C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of Chy10G181720 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 4.2e-149
Identity = 264/467 (56.53%), Postives = 324/467 (69.38%), Query Frame = 0


Query: 4   LSLLITLSLLATTAFADIGD-FDDYWKARAAAAEKAAADAYEPDP--------------- 63
           L  +I ++ L  T  A++ D  D+YW  +A  A K    AY PDP               
Sbjct: 9   LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68

Query: 64  ---------ETVANTVNAEIAHALSGRNSTRRNL------------KKYAGPCLATNPID 123
                    + VA+     I    S  NSTRR+L             K  GPC A+NPID
Sbjct: 69  TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128

Query: 124 RCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQ 183
           +CWRC P+WA+ RKKL  CV GFG +TTGGK G  YVV    D D++NP+PGTLRHAVIQ
Sbjct: 129 KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188

Query: 184 KGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQIAYGAGITLQYIRNVIIHGLRIHH 243
           K PLWI+F  +M+IRLSQEL++TSDKTIDARGANV IAYGAGIT+QY+ N+IIHGL +HH
Sbjct: 189 KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248

Query: 244 IVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCHDGLIDAIMGSTAIT 303
           IV  SGG+IRD+++H G R  +DGDGISIFG++N+W+DH+SMS C DGLIDAIMGSTAIT
Sbjct: 249 IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308

Query: 304 ISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYT 363
           ISN HFTHHN+VML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYT
Sbjct: 309 ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368

Query: 364 HWLMYAIGGSQHPTIVSQGNRFIAPPN-VYAKEVTKREYSPEQVWKSWTWRSEGDLMMNG 423
           HW +YAIGGSQ PTI+S GNRFIAPP+  + +EVTKR+Y+ E  WK+W WRSE D+ MN 
Sbjct: 369 HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428

Query: 424 AFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGSLGCFVGRPC 433
           A+F  SG+   +   SR  MI  K G  V ++T++AG+L C VG+ C
Sbjct: 429 AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Chy10G181720 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 8.0e-148
Identity = 259/464 (55.82%), Postives = 321/464 (69.18%), Query Frame = 0


Query: 4   LSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVAN----------- 63
           L     +++L  T   +I + D+YW  RA  A +    AY  DP  + +           
Sbjct: 9   LMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNST 68

Query: 64  --TVNAEIAHAL-------------SGRNSTRRNLK--------KYAGPCLATNPIDRCW 123
             T   E   A              S  NSTRR+L+        K  GPC A+NPID+CW
Sbjct: 69  DVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCW 128

Query: 124 RCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGP 183
           RC  +WA+ RKKL  CV GFG +TTGGK G  YVV  + D D++NPKPGTLRHAVIQK P
Sbjct: 129 RCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEP 188

Query: 184 LWIIFSTNMAIRLSQELIMTSDKTIDARGANVQIAYGAGITLQYIRNVIIHGLRIHHIVV 243
           LWIIF  +M+IRL+QEL++ S KTIDARGANV +A+GAGIT+Q+++NVIIHGL IHHI  
Sbjct: 189 LWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHISE 248

Query: 244 GSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCHDGLIDAIMGSTAITISN 303
            SGGMIRD+VDH G+RT +DGDG+SI+GSSN+W+DH+SMS C DGLIDAI+GST ITISN
Sbjct: 249 SSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISN 308

Query: 304 CHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 363
            HFTHHN+VML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 309 SHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWE 368

Query: 364 MYAIGGSQHPTIVSQGNRFIAPPN-VYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFF 423
           +YAIGGSQ PTI+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F
Sbjct: 369 LYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYF 428

Query: 424 VTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGSLGCFVGRPC 433
             SG+   +   +R  MI  K G  V ++T++AG+L C VGR C
Sbjct: 429 RQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Chy10G181720 vs. ExPASy TrEMBL
Match: A0A0A0KSL4 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G604340 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 9.5e-253
Identity = 423/432 (97.92%), Postives = 428/432 (99.07%), Query Frame = 0


Query: 1   MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
           M  LSLL+TLSLLATTAFADIGDFDDYWKARAAAAEKAAADAY+PDPETVAN+VNAEIAH
Sbjct: 1   MHTLSLLLTLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYDPDPETVANSVNAEIAH 60

Query: 61  ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
           A SGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP
Sbjct: 61  ASSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120

Query: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
           YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN
Sbjct: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180

Query: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
           VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240

Query: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
           VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQ+TVAFN
Sbjct: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQITVAFN 300

Query: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
           HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT
Sbjct: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360

Query: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
           KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYV+RMTRF
Sbjct: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVKRMTRF 420

Query: 421 AGSLGCFVGRPC 433
           AGSL CFVGRPC
Sbjct: 421 AGSLACFVGRPC 432

[Close]

BLAST of Chy10G181720 vs. ExPASy TrEMBL
Match: A0A5A7STF1 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003980 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.7e-241
Identity = 401/432 (92.82%), Postives = 416/432 (96.30%), Query Frame = 0


Query: 1   MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
           M  LSLL+TLSLLATTAFADIGDFDDYWKARAA AE+AAADAYEP+PET+A+++NAE+  
Sbjct: 1   MARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQ 60

Query: 61  ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
           ALSG NSTRRNLKKY GPC ATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKT GGKLGP
Sbjct: 61  ALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGP 120

Query: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
           YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI+TSDKTIDARGAN
Sbjct: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGAN 180

Query: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
           VQIAYGAGITLQY+RNVIIHGLRIHHIVVG+GGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 181 VQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240

Query: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
           VWIDHVSMSNC DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYS DQIMQVTVAFN
Sbjct: 241 VWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFN 300

Query: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
           HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT
Sbjct: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360

Query: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
           KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQ KRRPFS  DMISYKPGTYV+RMTRF
Sbjct: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRF 420

Query: 421 AGSLGCFVGRPC 433
           +G+L CFVGRPC
Sbjct: 421 SGALACFVGRPC 432

[Close]

BLAST of Chy10G181720 vs. ExPASy TrEMBL
Match: A0A5D3CZB5 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.7e-241
Identity = 401/432 (92.82%), Postives = 416/432 (96.30%), Query Frame = 0


Query: 1    MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
            M  LSLL+TLSLLATTAFADIGDFDDYWKARAA AE+AAADAYEP+PET+A+++NAE+  
Sbjct: 928  MARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQ 987

Query: 61   ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
            ALSG NSTRRNLKKY GPC ATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKT GGKLGP
Sbjct: 988  ALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGP 1047

Query: 121  YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
            YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI+TSDKTIDARGAN
Sbjct: 1048 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGAN 1107

Query: 181  VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
            VQIAYGAGITLQY+RNVIIHGLRIHHIVVG+GGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 1108 VQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSN 1167

Query: 241  VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
            VWIDHVSMSNC DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYS DQIMQVTVAFN
Sbjct: 1168 VWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFN 1227

Query: 301  HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
            HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT
Sbjct: 1228 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 1287

Query: 361  KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
            KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQ KRRPFS  DMISYKPGTYV+RMTRF
Sbjct: 1288 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRF 1347

Query: 421  AGSLGCFVGRPC 433
            +G+L CFVGRPC
Sbjct: 1348 SGALACFVGRPC 1359

[Close]

BLAST of Chy10G181720 vs. ExPASy TrEMBL
Match: A0A1S3BFK5 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489090 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.7e-241
Identity = 401/432 (92.82%), Postives = 416/432 (96.30%), Query Frame = 0


Query: 1   MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
           M  LSLL+TLSLLATTAFADIGDFDDYWKARAA AE+AAADAYEP+PET+A+++NAE+  
Sbjct: 1   MARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQ 60

Query: 61  ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
           ALSG NSTRRNLKKY GPC ATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKT GGKLGP
Sbjct: 61  ALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGP 120

Query: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
           YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI+TSDKTIDARGAN
Sbjct: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGAN 180

Query: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
           VQIAYGAGITLQY+RNVIIHGLRIHHIVVG+GGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 181 VQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240

Query: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
           VWIDHVSMSNC DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYS DQIMQVTVAFN
Sbjct: 241 VWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFN 300

Query: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
           HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT
Sbjct: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360

Query: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
           KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQ KRRPFS  DMISYKPGTYV+RMTRF
Sbjct: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRF 420

Query: 421 AGSLGCFVGRPC 433
           +G+L CFVGRPC
Sbjct: 421 SGALACFVGRPC 432

[Close]

BLAST of Chy10G181720 vs. ExPASy TrEMBL
Match: A0A6J1KK28 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493864 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.2e-223
Identity = 370/429 (86.25%), Postives = 401/429 (93.47%), Query Frame = 0


Query: 4   LSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAHALS 63
           L +L+TLSL+A TA+ADIG+FDDYWK+RAA AEKAAA A++P+PE V + +NAE+   LS
Sbjct: 10  LGVLLTLSLVA-TAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELS 69

Query: 64  GRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYV 123
           G N TRRNLK+Y GPCLATNPIDRCWRCDPNWA+NRKKLADCVLGFG KT GGKLGP+YV
Sbjct: 70  GSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYV 129

Query: 124 VNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQI 183
           VNDSSDSD+M PKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI++SDKTIDARGANVQI
Sbjct: 130 VNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQI 189

Query: 184 AYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWI 243
           AYGAGITLQY++NVIIHGLRIHHIV+GSGGMIRD+VDHVGLRTMSDGDGISIFGSSN+WI
Sbjct: 190 AYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWI 249

Query: 244 DHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFG 303
           DHVSMSNC DGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYS DQIMQVTVAFNHFG
Sbjct: 250 DHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFG 309

Query: 304 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKRE 363
           QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTKRE
Sbjct: 310 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKRE 369

Query: 364 YSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGS 423
           YSPE VWK+WTWRSEGDLMMNGAFFVTSGDQ K+RPFSRMDMISYK GTYV RMTR +G+
Sbjct: 370 YSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSRMDMISYKEGTYVTRMTRMSGA 429

Query: 424 LGCFVGRPC 433
           L CF+GRPC
Sbjct: 430 LPCFIGRPC 437

[Close]

BLAST of Chy10G181720 vs. NCBI nr
Match: XP_004135462.1 (probable pectate lyase P59 [Cucumis sativus] >KGN51889.1 hypothetical protein Csa_009201 [Cucumis sativus])

HSP 1 Score: 877 bits (2265), Expect = 0.0
Identity = 423/432 (97.92%), Postives = 428/432 (99.07%), Query Frame = 0


Query: 1   MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
           M  LSLL+TLSLLATTAFADIGDFDDYWKARAAAAEKAAADAY+PDPETVAN+VNAEIAH
Sbjct: 1   MHTLSLLLTLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYDPDPETVANSVNAEIAH 60

Query: 61  ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
           A SGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP
Sbjct: 61  ASSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120

Query: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
           YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN
Sbjct: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180

Query: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
           VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240

Query: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
           VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQ+TVAFN
Sbjct: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQITVAFN 300

Query: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
           HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT
Sbjct: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360

Query: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
           KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYV+RMTRF
Sbjct: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVKRMTRF 420

Query: 421 AGSLGCFVGRPC 432
           AGSL CFVGRPC
Sbjct: 421 AGSLACFVGRPC 432

[Close]

BLAST of Chy10G181720 vs. NCBI nr
Match: XP_008446316.1 (PREDICTED: probable pectate lyase P59 [Cucumis melo] >KAA0034392.1 putative pectate lyase P59 [Cucumis melo var. makuwa])

HSP 1 Score: 838 bits (2165), Expect = 4.21e-306
Identity = 401/432 (92.82%), Postives = 416/432 (96.30%), Query Frame = 0


Query: 1   MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
           M  LSLL+TLSLLATTAFADIGDFDDYWKARAA AE+AAADAYEP+PET+A+++NAE+  
Sbjct: 1   MARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQ 60

Query: 61  ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
           ALSG NSTRRNLKKY GPC ATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKT GGKLGP
Sbjct: 61  ALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGP 120

Query: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
           YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI+TSDKTIDARGAN
Sbjct: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGAN 180

Query: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
           VQIAYGAGITLQY+RNVIIHGLRIHHIVVG+GGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 181 VQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240

Query: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
           VWIDHVSMSNC DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYS DQIMQVTVAFN
Sbjct: 241 VWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFN 300

Query: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
           HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT
Sbjct: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360

Query: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
           KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQ KRRPFS  DMISYKPGTYV+RMTRF
Sbjct: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRF 420

Query: 421 AGSLGCFVGRPC 432
           +G+L CFVGRPC
Sbjct: 421 SGALACFVGRPC 432

[Close]

BLAST of Chy10G181720 vs. NCBI nr
Match: XP_038892739.1 (probable pectate lyase P59 [Benincasa hispida])

HSP 1 Score: 829 bits (2141), Expect = 1.92e-302
Identity = 397/432 (91.90%), Postives = 411/432 (95.14%), Query Frame = 0


Query: 1   MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
           M  L LL+TLS LA+TAFA+IG+ DDYWKARAA AE+AAADA+EP+PE V N  NA++  
Sbjct: 1   MAILRLLLTLSFLASTAFANIGNVDDYWKARAAEAEQAAADAFEPNPENVTNYFNAKVGQ 60

Query: 61  ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
            L G NSTRRNLKKY GPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFG KT GGKLGP
Sbjct: 61  VLIGTNSTRRNLKKYTGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGHKTIGGKLGP 120

Query: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
           YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRL+QELIMTSDKTIDARGAN
Sbjct: 121 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLTQELIMTSDKTIDARGAN 180

Query: 181 VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
           VQIAYGAGITLQYI+NVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 181 VQIAYGAGITLQYIKNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240

Query: 241 VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
           VWIDHVSMS CHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYS DQIMQVTVAFN
Sbjct: 241 VWIDHVSMSRCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFN 300

Query: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
           HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTI+SQGNRFIAPPNVYAKEVT
Sbjct: 301 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAKEVT 360

Query: 361 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
           KREYSPE+VWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF
Sbjct: 361 KREYSPEEVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420

Query: 421 AGSLGCFVGRPC 432
           AG+L CFVGRPC
Sbjct: 421 AGALACFVGRPC 432

[Close]

BLAST of Chy10G181720 vs. NCBI nr
Match: TYK15529.1 (alpha-amylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 838 bits (2165), Expect = 2.09e-292
Identity = 401/432 (92.82%), Postives = 416/432 (96.30%), Query Frame = 0


Query: 1    MPALSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAH 60
            M  LSLL+TLSLLATTAFADIGDFDDYWKARAA AE+AAADAYEP+PET+A+++NAE+  
Sbjct: 928  MARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQ 987

Query: 61   ALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGP 120
            ALSG NSTRRNLKKY GPC ATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKT GGKLGP
Sbjct: 988  ALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGP 1047

Query: 121  YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGAN 180
            YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI+TSDKTIDARGAN
Sbjct: 1048 YYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGAN 1107

Query: 181  VQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSN 240
            VQIAYGAGITLQY+RNVIIHGLRIHHIVVG+GGMIRDAVDHVGLRTMSDGDGISIFGSSN
Sbjct: 1108 VQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSN 1167

Query: 241  VWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFN 300
            VWIDHVSMSNC DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYS DQIMQVTVAFN
Sbjct: 1168 VWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFN 1227

Query: 301  HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 360
            HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT
Sbjct: 1228 HFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVT 1287

Query: 361  KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRF 420
            KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQ KRRPFS  DMISYKPGTYV+RMTRF
Sbjct: 1288 KREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRF 1347

Query: 421  AGSLGCFVGRPC 432
            +G+L CFVGRPC
Sbjct: 1348 SGALACFVGRPC 1359

[Close]

BLAST of Chy10G181720 vs. NCBI nr
Match: XP_022999568.1 (probable pectate lyase P59 [Cucurbita maxima])

HSP 1 Score: 780 bits (2013), Expect = 7.40e-283
Identity = 370/429 (86.25%), Postives = 401/429 (93.47%), Query Frame = 0


Query: 4   LSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAHALS 63
           L +L+TLSL+AT A+ADIG+FDDYWK+RAA AEKAAA A++P+PE V + +NAE+   LS
Sbjct: 10  LGVLLTLSLVAT-AYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELS 69

Query: 64  GRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYV 123
           G N TRRNLK+Y GPCLATNPIDRCWRCDPNWA+NRKKLADCVLGFG KT GGKLGP+YV
Sbjct: 70  GSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYV 129

Query: 124 VNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQI 183
           VNDSSDSD+M PKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI++SDKTIDARGANVQI
Sbjct: 130 VNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQI 189

Query: 184 AYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWI 243
           AYGAGITLQY++NVIIHGLRIHHIV+GSGGMIRD+VDHVGLRTMSDGDGISIFGSSN+WI
Sbjct: 190 AYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWI 249

Query: 244 DHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFG 303
           DHVSMSNC DGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYS DQIMQVTVAFNHFG
Sbjct: 250 DHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFG 309

Query: 304 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKRE 363
           QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTKRE
Sbjct: 310 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKRE 369

Query: 364 YSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGS 423
           YSPE VWK+WTWRSEGDLMMNGAFFVTSGDQ K+RPFSRMDMISYK GTYV RMTR +G+
Sbjct: 370 YSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSRMDMISYKEGTYVTRMTRMSGA 429

Query: 424 LGCFVGRPC 432
           L CF+GRPC
Sbjct: 430 LPCFIGRPC 437

[Close]

BLAST of Chy10G181720 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 540.8 bits (1392), Expect = 1.0e-153
Identity = 263/440 (59.77%), Postives = 317/440 (72.05%), Query Frame = 0


Query: 10  LSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEI---AHALSGRN 69
           L+++A     ++  FD YW  R + A K    +Y+P P  V N  N  +     A   RN
Sbjct: 20  LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 70  STRRNL---------KKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGK 129
            TRR L          K +G CLA NPID CWRCD NWA NRKKLADCVLGFGR+TTGGK
Sbjct: 80  DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 130 LGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDAR 189
            GP YVV D+SD+DL+NPKPGTLRHAV + GPLWIIF+ +M I+L QEL++TSDKTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 190 GANVQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFG 249
           GA V I  GAG+TLQ++ NVIIH + + HIV G+GG+IRD+  H+GLRT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 250 SSNVWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQVTV 309
           ++N+WIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D +  D+ MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 310 AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAK 369
           AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTI+SQGNRFIAPPN  AK
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 370 EVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSG-----DQSKRRPFSRMDMISYKPGT 429
           ++TKREY+P   WKSW W+SEGD  +NGA+FV SG         + P      I  KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 430 YVRRMTRFAGSLGCFVGRPC 433
            VR++T  AG LGC +G  C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of Chy10G181720 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 529.3 bits (1362), Expect = 3.0e-150
Identity = 264/467 (56.53%), Postives = 324/467 (69.38%), Query Frame = 0


Query: 4   LSLLITLSLLATTAFADIGD-FDDYWKARAAAAEKAAADAYEPDP--------------- 63
           L  +I ++ L  T  A++ D  D+YW  +A  A K    AY PDP               
Sbjct: 9   LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68

Query: 64  ---------ETVANTVNAEIAHALSGRNSTRRNL------------KKYAGPCLATNPID 123
                    + VA+     I    S  NSTRR+L             K  GPC A+NPID
Sbjct: 69  TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128

Query: 124 RCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQ 183
           +CWRC P+WA+ RKKL  CV GFG +TTGGK G  YVV    D D++NP+PGTLRHAVIQ
Sbjct: 129 KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188

Query: 184 KGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQIAYGAGITLQYIRNVIIHGLRIHH 243
           K PLWI+F  +M+IRLSQEL++TSDKTIDARGANV IAYGAGIT+QY+ N+IIHGL +HH
Sbjct: 189 KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248

Query: 244 IVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCHDGLIDAIMGSTAIT 303
           IV  SGG+IRD+++H G R  +DGDGISIFG++N+W+DH+SMS C DGLIDAIMGSTAIT
Sbjct: 249 IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308

Query: 304 ISNCHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYT 363
           ISN HFTHHN+VML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYT
Sbjct: 309 ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368

Query: 364 HWLMYAIGGSQHPTIVSQGNRFIAPPN-VYAKEVTKREYSPEQVWKSWTWRSEGDLMMNG 423
           HW +YAIGGSQ PTI+S GNRFIAPP+  + +EVTKR+Y+ E  WK+W WRSE D+ MN 
Sbjct: 369 HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428

Query: 424 AFFVTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGSLGCFVGRPC 433
           A+F  SG+   +   SR  MI  K G  V ++T++AG+L C VG+ C
Sbjct: 429 AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Chy10G181720 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 524.6 bits (1350), Expect = 7.4e-149
Identity = 258/464 (55.60%), Postives = 321/464 (69.18%), Query Frame = 0


Query: 4   LSLLITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVAN----------- 63
           L     +++L  T   +I + D+YW  RA  A +    AY  DP  + +           
Sbjct: 9   LMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNST 68

Query: 64  --TVNAEIAHAL-------------SGRNSTRRNLK--------KYAGPCLATNPIDRCW 123
             T   E   A              S  NSTRR+L+        K  GPC A+NPID+CW
Sbjct: 69  DVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCW 128

Query: 124 RCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGP 183
           RC  +WA+ RKKL  CV GFG +TTGGK G  YVV  + D D++NPKPGTLRHAVIQK P
Sbjct: 129 RCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEP 188

Query: 184 LWIIFSTNMAIRLSQELIMTSDKTIDARGANVQIAYGAGITLQYIRNVIIHGLRIHHIVV 243
           LWIIF  +M+IRL+QEL++ S KTIDARGANV +A+GAGIT+Q+++NV+IHGL IHHI  
Sbjct: 189 LWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISE 248

Query: 244 GSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCHDGLIDAIMGSTAITISN 303
            SGGMIRD+VDH G+RT +DGDG+SI+GSSN+W+DH+SMS C DGLIDAI+GST ITISN
Sbjct: 249 SSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISN 308

Query: 304 CHFTHHNEVMLFGASDGYSQDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 363
            HFTHHN+VML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 309 SHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWE 368

Query: 364 MYAIGGSQHPTIVSQGNRFIAPPN-VYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFF 423
           +YAIGGSQ PTI+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F
Sbjct: 369 LYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYF 428

Query: 424 VTSGDQSKRRPFSRMDMISYKPGTYVRRMTRFAGSLGCFVGRPC 433
             SG+   +   +R  MI  K G  V ++T++AG+L C VGR C
Sbjct: 429 RQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Chy10G181720 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.6e-143
Identity = 250/442 (56.56%), Postives = 319/442 (72.17%), Query Frame = 0


Query: 8   ITLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYEPDPETVANTVNAEIAHALSGR-- 67
           ++L+++  +  A I ++D+YW  R   A +   ++Y+P+PE V +  N   A A+     
Sbjct: 14  LSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAMETTGI 73

Query: 68  -NSTRRNLK---------KYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTG 127
            N TRR+L+         +  G   + N ID+CWR D NW +NRKKLADCVLGFGRKTTG
Sbjct: 74  VNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGRKTTG 133

Query: 128 GKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTID 187
           GK GP YVV D SD+DL+ PKPGT+RHAV +  PLWIIF+ +M I+L QELI+T+DKTID
Sbjct: 134 GKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITNDKTID 193

Query: 188 ARGANVQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISI 247
            RGA + I  GAG+TLQ++RNVIIH + I  I  G+GG+I D+  H GLRT+SDGDGI+I
Sbjct: 194 GRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGDGINI 253

Query: 248 FGSSNVWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQV 307
           FG++NVWIDHVSM++C DG+IDAIMGSTAITISN HFT H+EVMLFG ++    D+ MQ+
Sbjct: 254 FGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDKKMQI 313

Query: 308 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVY 367
           TVAFNHFG+ L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTI+SQGNRFIAPP   
Sbjct: 314 TVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPIED 373

Query: 368 AKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSG-----DQSKRRPFSRMDMISYKP 427
           +K+VTKREY+P   WKSW W+SE D  +NGA+FV SG       + + P  R   I  +P
Sbjct: 374 SKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAIRPQP 433

Query: 428 GTYVRRMTRFAGSLGCFVGRPC 433
           GT VRR+T+ AG+LGC  G+ C
Sbjct: 434 GTKVRRLTKDAGTLGCKPGKSC 455

[Close]

BLAST of Chy10G181720 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.1e-136
Identity = 232/391 (59.34%), Postives = 285/391 (72.89%), Query Frame = 0


Query: 42  AYEPDPETVANTVNAEIAHALSGRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKK 101
           A   DP+ VA+ V A++        + RR L  ++  C   NPID CWRCD NW +NRK+
Sbjct: 84  AVADDPDMVADEV-AKLVQMSEQNRTARRKLGFFS--CGTGNPIDDCWRCDRNWHKNRKR 143

Query: 102 LADCVLGFGRKTTGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIR 161
           LADC +GFGR   GG+ G +Y+V D +D D++NPKPGTLRHAVIQ+ PLWI+F  +M I 
Sbjct: 144 LADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIE 203

Query: 162 LSQELIMTSDKTIDARGANVQIAYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDH 221
           L QELIM S KTIDARG+NV IA GA IT+Q+I NVIIHGL IH        M+R +  H
Sbjct: 204 LKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSH 263

Query: 222 VGLRTMSDGDGISIFGSSNVWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLF 281
            G RTM+DGD +SIFGSS++WIDH S+S+C DGL+DA+MGSTAIT+SN HFTHHNEVML 
Sbjct: 264 FGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLL 323

Query: 282 GASDGYSQDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTI 341
           G SD Y++D++MQVT+A+NHFG+GLVQRMPRCR G+FHVVNNDYTHW MYAIGGS  PTI
Sbjct: 324 GHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTI 383

Query: 342 VSQGNRFIAPPNVYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFS 401
            SQGNR+ AP + +AKEVTKR  +    WK W WRSEGDL++NGAFF  SG       + 
Sbjct: 384 NSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG-AGASASYG 443

Query: 402 RMDMISYKPGTYVRRMTRFAGSLGCFVGRPC 433
           R   ++ KP + V  +T  AG+LGC  GRPC
Sbjct: 444 RASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:

BLAST of Chy10G181720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P15722	2.9e-166	64.71	Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1	[more]
P40973	1.4e-155	62.62	Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1	[more]
Q9M9S2	1.4e-152	59.77	Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1	[more]
Q9SRH4	4.2e-149	56.53	Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1	[more]
Q9LFP5	8.0e-148	55.82	Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1	[more]

BLAST of Chy10G181720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KSL4	9.5e-253	97.92	Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G604340 PE=3 SV=1	[more]
A0A5A7STF1	3.7e-241	92.82	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003980 ...	[more]
A0A5D3CZB5	3.7e-241	92.82	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 P...	[more]
A0A1S3BFK5	3.7e-241	92.82	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489090 PE=3 SV=1	[more]
A0A6J1KK28	1.2e-223	86.25	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493864 PE=3 SV=1	[more]

BLAST of Chy10G181720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135462.1	0.0	97.92	probable pectate lyase P59 [Cucumis sativus] >KGN51889.1 hypothetical protein Cs...	[more]
XP_008446316.1	4.21e-306	92.82	PREDICTED: probable pectate lyase P59 [Cucumis melo] >KAA0034392.1 putative pect...	[more]
XP_038892739.1	1.92e-302	91.90	probable pectate lyase P59 [Benincasa hispida]	[more]
TYK15529.1	2.09e-292	92.82	alpha-amylase 3 [Cucumis melo var. makuwa]	[more]
XP_022999568.1	7.40e-283	86.25	probable pectate lyase P59 [Cucurbita maxima]	[more]

BLAST of Chy10G181720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14420.1	1.0e-153	59.77	Pectate lyase family protein	[more]
AT3G01270.1	3.0e-150	56.53	Pectate lyase family protein	[more]
AT5G15110.1	7.4e-149	55.60	Pectate lyase family protein	[more]
AT2G02720.1	1.6e-143	56.56	Pectate lyase family protein	[more]
AT4G13710.1	1.1e-136	59.34	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 229..250 score: 66.45 coord: 309..328 score: 78.1 coord: 403..426 score: 39.68 coord: 331..350 score: 77.86 coord: 373..397 score: 52.38 coord: 165..181 score: 68.35 coord: 105..122 score: 57.94 coord: 129..154 score: 65.75
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 157..354 e-value: 5.4E-101 score: 351.5
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 169..348 e-value: 8.3E-21 score: 74.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 82..425 e-value: 2.9E-114 score: 383.6
IPR007524	Pectate lyase, N-terminal	PFAM	PF04431	Pec_lyase_N	coord: 21..72 e-value: 3.6E-17 score: 62.3
None	No IPR available	PANTHER	PTHR31683:SF132	PECTATE LYASE	coord: 6..432
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 6..432
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 83..422

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G181720.1	Chy10G181720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030570	pectate lyase activity