Chy10G179680 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G179680
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	4-coumarate--CoA ligase-like 1
Location	chrH10: 13818401 .. 13820821 (+)
RNA-Seq Expression	Chy10G179680
Synteny	Chy10G179680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAAATCTTCTTTTAAGTAGAGGGAAGTTGACGCCTGCAGAAAACTACTATTCTGCCATGGCTACTCGCAACCAAGATTCGGTACAAGACGAGGAACACATTTTCTGCAGCCAACTTCCTGAGGTCCAAGTGCCTTATGATATCACACTGCCTGAGTTTGTACTCCAGAATGCTGAATCATATGCCGAAAATGTGGCATTCGTGGAAGCCATGAGCGGGAAGGAATACACTTACCGTGAAGTTTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCGCTCTCTGAGGTTGAAGAAAGGACATGTGGTTATTGTTGTTCTACCTAATGTTGCTGAATACGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCAGGTGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCCAAACTTGTCGTCACAAATGCTGCAAACTTTGAAAAGGTAGGATAGAATTAAAAACTACGTTTGGTAACTTGATATCGAATGTATTTCTTTCGAGTTCATCGCTAACTATGTATGTTGTTGAGAACAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGGAACAATTTTGTTAAAGAGGAGATTAAGCAAACTGATTTATGTGCACTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGAGGGCAAGGTCACGACGCTAGGCCTCATTCCATTCTTCCATATTTATGGGATCACTGGAATATGTTGTTCTACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATACTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCACTCGCACCCGAACTTCAAACTGCCTTCGAGAGAAAATTCCCCGGTGTGGACGTTCAAGAGGTAATTTAGTTGTTACTCGAGTGTAGTAGATTGACTGTGACAACTCTCCATTCTCTGTTCATTTCTTCAAAAAATTTAGTTATGCCATATAGTCCAATTTCCTGTTTTCGACATGTGAGAAGCGAATCAAGCTCTACCATCACATTGCAGTGTTAATCTTTGGGGTTCTAACTTGTATAATATGCTAACTAATTGTCGTTGATCAACGACAGGCATATGGACTTACTGAGCACTGTTGCATCACTCTTAACTATGGAAATATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACACTGGAAGGTCTCTGCCCAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGTACAGAACATAGTTCGATGCAGAAAATTTTTACGGGATCTCTAATTAAATGCCAGCTGAATCTCTCATTGTTTTAGGCTACTACAAAAACGAAGAGGAGACTTCGAGGACGATCGATACCAAAGGCTGGATGCACACTGGGGATATTGGATACATCGATGACGATGGCAACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGATTTCAAGTAAAGACATAACATAACTTATTTCAAAGTAAAGACATAACATAACTTATTTCAAAGTGATCCATTGATTGATAAAAAGGAAGCTGAAGATGTGAAAAATGGAAGTTTGCATCAAACTTTTTTTGTATATGTGAACATGAAATAAGGTGATGGATGCATTGATTTCTCTGCAGGTTGCTCCGGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTAAGAATTCTCCCCTCTCATTCATTTCACTTCCTGTTGTTTCAATCTAAAGATGACAATGCTGTGCTGCATTGGCGATTCAGGTTGCCAGATGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAAAGAAACCGAAGAAGATATAATCAAACATGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGACTCGTTCATTTTGTAGACACTATCCCAAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGATTTATCTTCTAA

mRNA sequence

ATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAAATCTTCTTTTAAGTAGAGGGAAGTTGACGCCTGCAGAAAACTACTATTCTGCCATGGCTACTCGCAACCAAGATTCGGTACAAGACGAGGAACACATTTTCTGCAGCCAACTTCCTGAGGTCCAAGTGCCTTATGATATCACACTGCCTGAGTTTGTACTCCAGAATGCTGAATCATATGCCGAAAATGTGGCATTCGTGGAAGCCATGAGCGGGAAGGAATACACTTACCGTGAAGTTTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCGCTCTCTGAGGTTGAAGAAAGGACATGTGGTTATTGTTGTTCTACCTAATGTTGCTGAATACGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCAGGTGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCCAAACTTGTCGTCACAAATGCTGCAAACTTTGAAAAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGGAACAATTTTGTTAAAGAGGAGATTAAGCAAACTGATTTATGTGCACTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGAGGGCAAGGTCACGACGCTAGGCCTCATTCCATTCTTCCATATTTATGGGATCACTGGAATATGTTGTTCTACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATACTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCACTCGCACCCGAACTTCAAACTGCCTTCGAGAGAAAATTCCCCGGTGTGGACGTTCAAGAGGCATATGGACTTACTGAGCACTGTTGCATCACTCTTAACTATGGAAATATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACACTGGAAGGTCTCTGCCCAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGCTACTACAAAAACGAAGAGGAGACTTCGAGGACGATCGATACCAAAGGCTGGATGCACACTGGGGATATTGGATACATCGATGACGATGGCAACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGATTTCAAGTTGCTCCGGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTTGCCAGATGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAAAGAAACCGAAGAAGATATAATCAAACATGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGACTCGTTCATTTTGTAGACACTATCCCAAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGATTTATCTTCTAA

Coding sequence (CDS)

ATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAAATCTTCTTTTAAGTAGAGGGAAGTTGACGCCTGCAGAAAACTACTATTCTGCCATGGCTACTCGCAACCAAGATTCGGTACAAGACGAGGAACACATTTTCTGCAGCCAACTTCCTGAGGTCCAAGTGCCTTATGATATCACACTGCCTGAGTTTGTACTCCAGAATGCTGAATCATATGCCGAAAATGTGGCATTCGTGGAAGCCATGAGCGGGAAGGAATACACTTACCGTGAAGTTTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCGCTCTCTGAGGTTGAAGAAAGGACATGTGGTTATTGTTGTTCTACCTAATGTTGCTGAATACGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCAGGTGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCCAAACTTGTCGTCACAAATGCTGCAAACTTTGAAAAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGGAACAATTTTGTTAAAGAGGAGATTAAGCAAACTGATTTATGTGCACTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGAGGGCAAGGTCACGACGCTAGGCCTCATTCCATTCTTCCATATTTATGGGATCACTGGAATATGTTGTTCTACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATACTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCACTCGCACCCGAACTTCAAACTGCCTTCGAGAGAAAATTCCCCGGTGTGGACGTTCAAGAGGCATATGGACTTACTGAGCACTGTTGCATCACTCTTAACTATGGAAATATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACACTGGAAGGTCTCTGCCCAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGCTACTACAAAAACGAAGAGGAGACTTCGAGGACGATCGATACCAAAGGCTGGATGCACACTGGGGATATTGGATACATCGATGACGATGGCAACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGATTTCAAGTTGCTCCGGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTTGCCAGATGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAAAGAAACCGAAGAAGATATAATCAAACATGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGACTCGTTCATTTTGTAGACACTATCCCAAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGATTTATCTTCTAA

Protein sequence

MPISPSRTLQVSHSQSMIQPFSNLLLSRGKLTPAENYYSAMATRNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF*

Homology

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BLAST of Chy10G179680 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 1.0e-222
Identity = 385/532 (72.37%), Postives = 451/532 (84.77%), Query Frame = 0


Query: 50  QDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRF 109
           +D E+IF S  P V +P  +TLPEFVLQ  E Y ENVAFVEA++GK  TY +V+RDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 110 SKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEA 169
           +KAL SL L+KG V++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 170 KLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCA 229
           + ++T+A N+EKV+ L LPVI+L EE +EGA+NW  LLEA D+ G+    EEI QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 230 LPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCS 289
           LPFSSGTTG+ KGVMLTHRNL+AN+CSTL GV  EM G++ TLGLIPFFHIYGI GICC+
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 290 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 349
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 350 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 409
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEH CITL +G+  K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 410 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYID 469
           NLEVKFIDPDTGRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 470 DDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 529
           DDG++FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 530 APNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 582
            P + E EEDI+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

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BLAST of Chy10G179680 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 694.5 bits (1791), Expect = 1.0e-198
Identity = 344/539 (63.82%), Postives = 437/539 (81.08%), Query Frame = 0


Query: 50  QDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMS-GKEYTYREVLRDTNR 109
           +++EH+F S+ P V VP  +T+PEFVL  AE+YA+ VA VEA + G+ YTY EV RDT R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 110 FSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAE 169
           F++ALRS+ ++KGHVV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE +E
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 170 AKLVVTNAANFEKVRELKLPVI-LLEEELMEGAMNWHKLLEAADRAGNNFVK-EEIKQTD 229
           AKLVV N   F+KV++  +PVI + + E M GA++W  LL AADR G   V  +  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 230 LCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGI 289
           LCALP+SSGTTGVSKGVML+HRNLV+N+CS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 290 CCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKL 349
           CC+TLR+KG VVVM RFDLRTF+ AL+ H + FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 350 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGR 409
           +++MTAAAPLAP+L  AF+RKFPGV V+EAYGLTEH CITL +         AKK++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 410 ILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIG 469
           ILPNLEVKF+DPDTGRSLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 470 YIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 529
           YID DG+VFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 530 VVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIR 586
           VV    ++E EE+I+ +VA  VA YK+VR++H VD IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

[Close]

BLAST of Chy10G179680 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 3.3e-112
Identity = 203/539 (37.66%), Postives = 331/539 (61.41%), Query Frame = 0


Query: 47  DSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDT 106
           ++ Q  + IF S+LP++ +P  + L  +  +N   +      ++  + + YTY EV   +
Sbjct: 6   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTS 65

Query: 107 NRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEV 166
            + +  L  L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + 
Sbjct: 66  RKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 125

Query: 167 AEAKLVVTNAANFEKVRELKLP---VILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIK 226
           + AK+V+T A    KV++  +     ++  + + EG +++ +L+++ +   +     +I+
Sbjct: 126 SSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDE---HEIPDVKIQ 185

Query: 227 QTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIY 286
             D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY
Sbjct: 186 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 245

Query: 287 GITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDL 346
            +  +    LR    +++M +FD+  F+  +  H++T  P VPPI+LA+ K+P+V+ +DL
Sbjct: 246 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 305

Query: 347 SSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKK 406
           SS++   +M+ AAPL  EL+ A   KFP   + + YG+TE   +        KE    K 
Sbjct: 306 SSVR--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 365

Query: 407 NTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMH 466
              G ++ N E+K +DPDTG SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+H
Sbjct: 366 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLH 425

Query: 467 TGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGE 526
           TGDIG+IDDD  +FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE
Sbjct: 426 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGE 485

Query: 527 IPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           +P A VV +  S  TE+++   ++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 486 VPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

[Close]

BLAST of Chy10G179680 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 5.7e-112
Identity = 209/534 (39.14%), Postives = 329/534 (61.61%), Query Frame = 0


Query: 52  EEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSK 111
           E+ IF S+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + + 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 112 ALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKL 171
            L  L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ ++AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 172 VVTNAANFEKVREL---KLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLC 231
           ++T A   +KV++    K   I+  ++  +  +++ KL+EA +   +   +  I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADE---SEMPEVVINSDDVV 188

Query: 232 ALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGI 291
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 292 CCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKL 351
            C  LR    +++M +FD+  F+  +  +++T  P VPPI+LA+ K+P+V+++DLSS++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 352 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGR 411
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 412 ILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIG 471
           ++ N E+K +DP+T  SLP+N  GEIC+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 472 YIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 531
           +IDDD  +FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 532 VVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           VV       TEE+I + V+  V  YK++  V FVD IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

[Close]

BLAST of Chy10G179680 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 9.7e-112
Identity = 203/539 (37.66%), Postives = 329/539 (61.04%), Query Frame = 0


Query: 47  DSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDT 106
           ++ Q  + IF S+LP++ +P  + L  +  +N   +      ++  + + YTY EV   +
Sbjct: 6   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTS 65

Query: 107 NRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEV 166
            + +  L  L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + 
Sbjct: 66  RKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 125

Query: 167 AEAKLVVTNAANFEKVRELKLP---VILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIK 226
           + AK+V+T A    KV++  +     ++  +   EG +++ +L+++ +   +     +I+
Sbjct: 126 SSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDE---HEIPDVKIQ 185

Query: 227 QTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIY 286
             D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY
Sbjct: 186 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 245

Query: 287 GITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDL 346
            +  +    LR    +++M +FD+  F+  +  H++T  P VPPI+LA+ K+P+V  +DL
Sbjct: 246 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 305

Query: 347 SSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKK 406
           SS++   +M+ AAPL  EL+ A   KFP   + + YG+TE   +        KE    K 
Sbjct: 306 SSVR--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 365

Query: 407 NTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMH 466
              G ++ N E+K +DPDTG SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+H
Sbjct: 366 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLH 425

Query: 467 TGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGE 526
           TGDIG+IDDD  +FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE
Sbjct: 426 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGE 485

Query: 527 IPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           +P A VV +  S  TE+++   ++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 486 VPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

[Close]

BLAST of Chy10G179680 vs. ExPASy TrEMBL
Match: A0A1S3BER3 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 577/596 (96.81%), Postives = 583/596 (97.82%), Query Frame = 0


Query: 1   MPISPSRTLQVSHSQSMIQPFSNLLLSRGKL--TPAENYYSAMATRNQDSVQDEEHIFCS 60
           MPISPSRTLQVSHSQSMIQPFS LLLSRGKL    AENYYSAM TRNQDSVQDE+HIF S
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYAENVAFVEAMSGK YTYREVLRDTNRFSKAL SLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKGHVV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 595
           +IIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596

[Close]

BLAST of Chy10G179680 vs. ExPASy TrEMBL
Match: A0A5D3CV41 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002330 PE=4 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 567/580 (97.76%), Query Frame = 0


Query: 17  MIQPFSNLLLSRGKL--TPAENYYSAMATRNQDSVQDEEHIFCSQLPEVQVPYDITLPEF 76
           MIQPFS LLLSRGKL    AENYYSAM TRNQDSVQDE+HIF SQLPEVQVP DITLPEF
Sbjct: 1   MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 60

Query: 77  VLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEY 136
           VLQNAESYAENVAFVEAMSGK YTYREVLRDTNRFSKAL SLRLKKGHVV+VVLPNVAEY
Sbjct: 61  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 120

Query: 137 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 196
           AIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE
Sbjct: 121 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 180

Query: 197 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 256
           ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM
Sbjct: 181 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 240

Query: 257 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 316
           CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 300

Query: 317 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 376
           HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ
Sbjct: 301 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 360

Query: 377 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 436
           EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 420

Query: 437 RSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPA 496
           RSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 497 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKV 556
           ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE+IIKHVASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 540

Query: 557 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 595
           RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 580

[Close]

BLAST of Chy10G179680 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 551/554 (99.46%), Postives = 552/554 (99.64%), Query Frame = 0


Query: 41  MATRNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYR 100
           MAT NQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGK YTYR
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 101 EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 160
           EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 161 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 220
           KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 221 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 280
           EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 281 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 340
           YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 341 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAK 400
           LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYG+IGKENLSAK
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 401 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 460
           KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 461 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 520
           HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 521 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 580
           EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 581 IEKIRADESARFIF 595
           IEKIRADESARFIF
Sbjct: 541 IEKIRADESARFIF 554

[Close]

BLAST of Chy10G179680 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.6e-293
Identity = 518/592 (87.50%), Postives = 560/592 (94.59%), Query Frame = 0


Query: 1   MPISPSRTLQVSHSQSMIQPFSNLLLSRGKLT--PAENYYSAMATRNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+ +   A N  S MAT  +DS +DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATCIRDSAEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAE YA+NVAFVEA+SGK YTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG +VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSG+PQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLSSLKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYGNIGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 591
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

[Close]

BLAST of Chy10G179680 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.4e-293
Identity = 520/592 (87.84%), Postives = 560/592 (94.59%), Query Frame = 0


Query: 1   MPISPSRTLQVSHSQSMIQPFSNLLLSRGK--LTPAENYYSAMATRNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+     A N  SAMAT  +D V+DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SGK YTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG VVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYG+IGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 591
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 606

[Close]

BLAST of Chy10G179680 vs. NCBI nr
Match: XP_004135516.2 (4-coumarate--CoA ligase-like 1 [Cucumis sativus] >KAE8648676.1 hypothetical protein Csa_008120 [Cucumis sativus])

HSP 1 Score: 1157 bits (2993), Expect = 0.0
Identity = 590/594 (99.33%), Postives = 591/594 (99.49%), Query Frame = 0


Query: 1   MPISPSRTLQVSHSQSMIQPFSNLLLSRGKLTPAENYYSAMATRNQDSVQDEEHIFCSQL 60
           MPISPSRTLQVSHSQSMIQPFS LLLSRGKLTPAENYYSAMAT NQDSVQDEEHIFCSQL
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLTPAENYYSAMATCNQDSVQDEEHIFCSQL 60

Query: 61  PEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRLKK 120
           PEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGK YTYREVLRDTNRFSKALRSLRLKK
Sbjct: 61  PEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRLKK 120

Query: 121 GHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFE 180
           GHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFE
Sbjct: 121 GHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFE 180

Query: 181 KVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVS 240
           KVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVS
Sbjct: 181 KVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVS 240

Query: 241 KGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVM 300
           KGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVM
Sbjct: 241 KGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVM 300

Query: 301 GRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPEL 360
           GRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPEL
Sbjct: 301 GRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPEL 360

Query: 361 QTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDT 420
           QTAFERKFPGVDVQEAYGLTEHCCITLNYG+IGKENLSAKKNTVGRILPNLEVKFIDPDT
Sbjct: 361 QTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDT 420

Query: 421 GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRI 480
           GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRI
Sbjct: 421 GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRI 480

Query: 481 KELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDI 540
           KELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDI
Sbjct: 481 KELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDI 540

Query: 541 IKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 594
           IKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF
Sbjct: 541 IKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 594

[Close]

BLAST of Chy10G179680 vs. NCBI nr
Match: XP_008446022.1 (PREDICTED: 4-coumarate--CoA ligase-like 1 [Cucumis melo])

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 577/596 (96.81%), Postives = 583/596 (97.82%), Query Frame = 0


Query: 1   MPISPSRTLQVSHSQSMIQPFSNLLLSRGKL--TPAENYYSAMATRNQDSVQDEEHIFCS 60
           MPISPSRTLQVSHSQSMIQPFS LLLSRGKL    AENYYSAM TRNQDSVQDE+HIF S
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYAENVAFVEAMSGK YTYREVLRDTNRFSKAL SLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKGHVV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 594
           +IIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596

[Close]

BLAST of Chy10G179680 vs. NCBI nr
Match: KAA0034187.1 (4-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa] >TYK15733.1 4-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1097 bits (2837), Expect = 0.0
Identity = 561/580 (96.72%), Postives = 567/580 (97.76%), Query Frame = 0


Query: 17  MIQPFSNLLLSRGKL--TPAENYYSAMATRNQDSVQDEEHIFCSQLPEVQVPYDITLPEF 76
           MIQPFS LLLSRGKL    AENYYSAM TRNQDSVQDE+HIF SQLPEVQVP DITLPEF
Sbjct: 1   MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 60

Query: 77  VLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEY 136
           VLQNAESYAENVAFVEAMSGK YTYREVLRDTNRFSKAL SLRLKKGHVV+VVLPNVAEY
Sbjct: 61  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 120

Query: 137 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 196
           AIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE
Sbjct: 121 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 180

Query: 197 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 256
           ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM
Sbjct: 181 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 240

Query: 257 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 316
           CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 300

Query: 317 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 376
           HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ
Sbjct: 301 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 360

Query: 377 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 436
           EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 420

Query: 437 RSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPA 496
           RSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 497 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKV 556
           ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE+IIKHVASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 540

Query: 557 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 594
           RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 580

[Close]

BLAST of Chy10G179680 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1020 bits (2637), Expect = 0.0
Identity = 518/592 (87.50%), Postives = 560/592 (94.59%), Query Frame = 0


Query: 1   MPISPSRTLQVSHSQSMIQPFSNLLLSRGKLTP--AENYYSAMATRNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+ +   A N  S MAT  +DS +DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATCIRDSAEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAE YA+NVAFVEA+SGK YTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG +VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSG+PQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLSSLKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYGNIGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 590
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

[Close]

BLAST of Chy10G179680 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 520/592 (87.84%), Postives = 560/592 (94.59%), Query Frame = 0


Query: 1   MPISPSRTLQVSHSQSMIQPFSNLLLSRGK--LTPAENYYSAMATRNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+  L  A    SAMAT  +DSV+DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRSLMSAATNNSAMATCIRDSVEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SG  YTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGNAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG VVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYG+IGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 590
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

[Close]

BLAST of Chy10G179680 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 774.2 bits (1998), Expect = 7.4e-224
Identity = 385/532 (72.37%), Postives = 451/532 (84.77%), Query Frame = 0


Query: 50  QDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRF 109
           +D E+IF S  P V +P  +TLPEFVLQ  E Y ENVAFVEA++GK  TY +V+RDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 110 SKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEA 169
           +KAL SL L+KG V++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 170 KLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCA 229
           + ++T+A N+EKV+ L LPVI+L EE +EGA+NW  LLEA D+ G+    EEI QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 230 LPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCS 289
           LPFSSGTTG+ KGVMLTHRNL+AN+CSTL GV  EM G++ TLGLIPFFHIYGI GICC+
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 290 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 349
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 350 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 409
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEH CITL +G+  K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 410 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYID 469
           NLEVKFIDPDTGRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 470 DDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 529
           DDG++FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 530 APNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 582
            P + E EEDI+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

[Close]

BLAST of Chy10G179680 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 391.7 bits (1005), Expect = 1.0e-108
Identity = 201/543 (37.02%), Postives = 329/543 (60.59%), Query Frame = 0


Query: 44  RNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVL 103
           +N       + IF S+LP++ +P  + L +++ +N   +A     +   +G+ YTY +V 
Sbjct: 11  QNDQKQCSNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVH 70

Query: 104 RDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQ 163
             + + +  L +L +K+  VV+++LPN  E  +  L     G + +  NP    +EI KQ
Sbjct: 71  VTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQ 130

Query: 164 VEVAEAKLVVTNAANFEKVRELKLPVILL----EEELMEGAMNWHKLLEAADRAGNNFVK 223
            + + AKL+VT +   +K++ L+   +L+     + + E  + + +L ++ +   ++ + 
Sbjct: 131 AKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDS-IP 190

Query: 224 EEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEM--EGKVTTLGLIPF 283
           E+I   D+ ALPFSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P 
Sbjct: 191 EKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPM 250

Query: 284 FHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVE 343
           FHIY +  I   +LR    +++M +F++   +  +   ++T A +VPPI+LA+ K+P  E
Sbjct: 251 FHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETE 310

Query: 344 EFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENL 403
           ++DLSS+++  + + AAPL  EL+ A   KFP   + + YG+TE   +        KE  
Sbjct: 311 KYDLSSVRM--VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPF 370

Query: 404 SAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTK 463
             K    G ++ N E+K +DPDTG SLP+N PGEIC+R   +M+GY  +   T+ TID  
Sbjct: 371 PVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKD 430

Query: 464 GWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDE 523
           GW+HTGD+G+IDDD  +FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +E
Sbjct: 431 GWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEE 490

Query: 524 EAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIK 581
           +AGE+P A VV + +S  +E++I + V+  V  YK++  V F D+IPK+PSGK++R+ ++
Sbjct: 491 DAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLR 550

[Close]

BLAST of Chy10G179680 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 387.1 bits (993), Expect = 2.5e-107
Identity = 204/560 (36.43%), Postives = 328/560 (58.57%), Query Frame = 0


Query: 31  LTPAENYYSAMATRNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVE 90
           + P E   S +  + Q +  + + IF S+LP++ +P  ++L +++ QN   +A     + 
Sbjct: 1   MAPQEQAVSQVMEK-QSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLIN 60

Query: 91  AMSGKEYTYREVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSG 150
             +G  YTY +V   + + +     L + +  VV+++LPN  E+ +  L     G   + 
Sbjct: 61  GPTGHVYTYSDVHVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATA 120

Query: 151 VNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELK----LPVILLEEE----LMEGAMN 210
            NP    +EI KQ + +  KL++T A   +K++ L+    + ++ +++     + EG + 
Sbjct: 121 ANPFFTPAEIAKQAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLR 180

Query: 211 WHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVP 270
           + +L ++   A       EI   D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G  
Sbjct: 181 FTELTQSTTEASEVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN 240

Query: 271 QEM--EGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFA 330
             +        L ++P FHIY +  I    LR    +++M +F++   +  +   ++T A
Sbjct: 241 PNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVA 300

Query: 331 PIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLT 390
           P+VPPI+LA+ K+   E++DLSS+++  + + AAPL  EL+ A   KFP   + + YG+T
Sbjct: 301 PMVPPIVLAIAKSSETEKYDLSSIRV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMT 360

Query: 391 EHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVM 450
           E   +        KE    K    G ++ N E+K +DPDTG SL +N PGEIC+R   +M
Sbjct: 361 EAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIM 420

Query: 451 QGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAIL 510
           +GY  N   T+ TID  GW+HTGDIG IDDD  +FIVDR+KELIKYKGFQVAPAELEA+L
Sbjct: 421 KGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALL 480

Query: 511 LTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFV 570
           + HP I D AVV + +E AGE+P A VV + +S+ +E+D+ + V+  V  YK++  V F 
Sbjct: 481 IGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFT 540

Query: 571 DTIPKSPSGKVMRRLIKEKM 581
           ++IPK+PSGK++R+ ++ K+
Sbjct: 541 ESIPKAPSGKILRKDLRAKL 557

[Close]

BLAST of Chy10G179680 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 380.6 bits (976), Expect = 2.4e-105
Identity = 204/531 (38.42%), Postives = 321/531 (60.45%), Query Frame = 0


Query: 55  IFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKALR 114
           IF S+LP++ +P  + L  +  +   S ++    +   +GK YTY E      R +  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 115 SLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 174
            L ++KG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 175 NAANFEKVREL--KLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE-EIKQTDLCALP 234
           ++   +K++ L   L +I  +E   E  + +  L+   D   N F +  +I   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 235 FSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCS 294
           FSSGTTG+ KGV+LTH++L+ ++   + G      ++     L ++P FHIY +  +  +
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 295 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 354
           +LR+   V++M +F++   ++ +  H +T A +VPP+++AL KNP V  +DLSS++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 355 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 414
           ++ AAPL  ELQ +  R+ P   + + YG+TE   +        KE +  K  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 415 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYID 474
           N E+K +  +T  SL  N PGEIC+R Q +M+ Y  + E TS TID +GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 475 DDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 534
           +D  +FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  DE AGE+P A VV 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 535 APNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           +  +  TEED+ ++VA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of Chy10G179680 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 377.1 bits (967), Expect = 2.6e-104
Identity = 210/543 (38.67%), Postives = 327/543 (60.22%), Query Frame = 0


Query: 55  IFCSQLPEVQVPYD--ITLPEFVLQNAESYAENVAFVEAMSGKEYTYREVLRDTNRFSKA 114
           I+ S  P + +P D   +L  F+ +N+ SY   +A  ++ +G   T+ ++     R +  
Sbjct: 11  IYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG 70

Query: 115 LRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLV 174
              L ++K  VV++  PN  ++ +  L + A GGVF+  NP   ++E+ KQ++ +  K++
Sbjct: 71  FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII 130

Query: 175 VTNAANFEKVRELKLPVILLEE----ELMEGA----MNWHKLLEAADRAGNNFVKEEIKQ 234
           ++    F+K++   LPV+LL      E+  G+    +++  ++E ++   + +   EIKQ
Sbjct: 131 ISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPV-SEYPFVEIKQ 190

Query: 235 TDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKV--TTLGLIPFFHIYG 294
           +D  AL +SSGTTG SKGV LTH N +A   S +  + Q++ G+     L  +P FH++G
Sbjct: 191 SDTAALLYSSGTTGTSKGVELTHGNFIA--ASLMVTMDQDLMGEYHGVFLCFLPMFHVFG 250

Query: 295 ITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLS 354
           +  I  S L+    +V M RF+L   +  +    +T   +VPP+ LAL K  IV++FDLS
Sbjct: 251 LAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFDLS 310

Query: 355 SLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHC-CITLNYGNIGKENLSAKK 414
           SLK   I + AAPL  +L     R  P V + + YG+TE C  +++    +GK N     
Sbjct: 311 SLKY--IGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN----S 370

Query: 415 NTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMH 474
            + G + P +E + +  +TG+S P N  GEI VR   +M+GY  N + T  TID K W+H
Sbjct: 371 GSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVH 430

Query: 475 TGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGE 534
           TGD+GY ++DGN+++VDRIKELIKYKGFQVAPAELE +L++HP I DA V+P PDEEAGE
Sbjct: 431 TGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGE 490

Query: 535 IPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 585
           +P A VV +PNS  TE+DI K +A  VA YK++R V F+  +PKS +GK++RR + +++ 
Sbjct: 491 VPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVR 544

The following BLAST results are available for this feature:

BLAST of Chy10G179680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQ12	1.0e-222	72.37	4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=...	[more]
Q7XXL2	1.0e-198	63.82	4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9...	[more]
P31684	3.3e-112	37.66	4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1	[more]
P14912	5.7e-112	39.14	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1	[more]
P31685	9.7e-112	37.66	4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1	[more]

BLAST of Chy10G179680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BER3	0.0e+00	96.81	4-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1	[more]
A0A5D3CV41	0.0e+00	96.72	4-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A0A0KRZ5	0.0e+00	99.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1	[more]
A0A6J1HUV5	2.6e-293	87.50	4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ...	[more]
A0A6J1G0B4	4.4e-293	87.84	4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=...	[more]

BLAST of Chy10G179680 vs. NCBI nr


Match Name	Identity	Description
XP_004135516.2	99.33	4-coumarate--CoA ligase-like 1 [Cucumis sativus] >KAE8648676.1 hypothetical prot...	[more]
XP_008446022.1	96.81	PREDICTED: 4-coumarate--CoA ligase-like 1 [Cucumis melo]	[more]
KAA0034187.1	96.72	4-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa] >TYK15733.1 4-coumarat...	[more]
XP_022966799.1	87.50	4-coumarate--CoA ligase-like 1 [Cucurbita maxima]	[more]
XP_023541569.1	87.84	4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy10G179680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G62940.1	7.4e-224	72.37	acyl-CoA synthetase 5	[more]
AT3G21240.1	1.0e-108	37.02	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	2.5e-107	36.43	4-coumarate:CoA ligase 1	[more]
AT1G65060.1	2.4e-105	38.42	4-coumarate:CoA ligase 3	[more]
AT4G05160.1	2.6e-104	38.67	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 495..570 e-value: 9.5E-18 score: 65.0
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 53..481 e-value: 1.0E-132 score: 444.9
None	No IPR available	GENE3D	3.30.300.30		coord: 482..584 e-value: 1.9E-31 score: 110.7
None	No IPR available	PANTHER	PTHR24096:SF389	4-COUMARATE--COA LIGASE-LIKE 1	coord: 47..583
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 47..583
None	No IPR available	CDD	cd05904	4CL	coord: 65..574 e-value: 0.0 score: 716.321
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 52..583
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 77..486 e-value: 9.0E-99 score: 330.9
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 230..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G179680.1	Chy10G179680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046949	fatty-acyl-CoA biosynthetic process
biological_process	GO:0001676	long-chain fatty acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004467	long-chain fatty acid-CoA ligase activity