Chy10G178690 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G178690
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Alpha-galactosidase
Location	chrH10: 13201261 .. 13204852 (+)
RNA-Seq Expression	Chy10G178690
Synteny	Chy10G178690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTTCCCCCATGTTCACCTTCAGTTATGCTTCTACTCTACTTCCTCCTTTTCTGGACGTTCTTGCTAGGTGGCAATGGCAATGGCAATGGCAATGGCGTTGTGGTTGCCGGAGCTTCTCGATCGACAGCTCTACGGTTTGCCACTGAGTTTGATTCTGCTTCTTCTAGGAGAGTTCTTCTTAACAATGGTCTCGCTCTAACTCCTCCGATGGGGTAAGTGTTATGCTCCCATCATGATTTTTAAGCAGAATCGATTTGATTGAATCAAATGTTGTTGTTTTTTTTTTTTTTTGTTGTTGATCTTTTTGGCTTTGTTCAATAGTGTTTTTTTGATAATTTGATGGTGATGGGTTGCTGATTTTTGTAATCAACGCAGATGGAATAGTTGGAACCATTTTCAATGTAATTTAAACGAGAATTTAATCAAGGAAACAGGTGATGATTTTGATTTGAAGGCGTTTTTCTATTTCGATTTGTATACTGTGAGTTTGTGACTGAGATACACATACTATTTATTTTGCTAAGTGGATGCAATGGTATCCACCGGACTTGCTGCATTAGGATATGGGTATATCAATTTAGGTGATGATTTTATTTTTTGTACATTTCTTTGGTTTTTCGACTTCATGCTTTCTGTTTTTGACATTAATATGTTCTTGTTTCCATATGTATTAGATGATTGTTGGGCTGAACTTGACAGAGACTCTAAGGTAAATTTGAATGTTCTATTTCCTGTCTAGGATGTCTATGAAAATCTCTATAATTGCATTAAGTGATTTCCATCAAAATTTTCAGGGTAATTTGGTTGCTAAAGCTTCAACGTTCCCTTCTGGTATTAAGGCCCTAGCAGATTATGTTCATAGAAAAGGGCTCAAGCTTGGAATTTATTCGGATGCTGGGTAAGTTTATACACTCATCAGATCAGCTTAAAATACTTCCTGATCTTTATATTTCAATCTTCTTTACTTAAGTTGTATAATTTCTTCAGGATCCAAACTTGCAGTAAAAGGATGCCTGGTTCCCTTGGTCACGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGTAATTTTTGCTGTTCTAGTCTACACTCTTAAAGCTCAACTCATTTAATTCTTGTTTATAATGAATGATTTTTTTCTTAGAAACTGATATATACATAATTGTACAGGGGATTGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGGTAGGATTAGCTTTCTACGTTCAAGTATCAAAATTTCCAAATGACTACAACAATTCCAGGGGTACAGATATCCAAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCATTATGCGAATGGTTAGGAATCTTTTATACACAAAAAAAAAAAAATGCTATAGTTGTTCTAATACTTCTCCATCCTAAAACACAGACCGAGTTCCTCTCTTAAATTTTTCCTCATGTAGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACAACATCAGATATTCAAGATAACTGGATTAGGTAATGAGCATAAATTCGATCATCATTTCTGGAAACACTCAGTTTTTTGGAAAACTGTCTACACTATTTGATTAGTCCCTTGTGTCTATCTATGAATATGCCTGTACTGAAGTACGATCTTTCAAGTCCAAAAATACTTTCTTTAGCAAGAAACTAATGCTCCTAATTAATGATTACAGCATGATAACCATTGCTGATCAGAATGACAAATGGGCTTCTTACGCTAAACCAGGAGGATGGAATGGTAAATTCATAATTGTTGCTCAATATGATAATTAAACACTGGCTGGTTAGCTTTTCGGTACTTAGTAAAGATTCGAAGGATTAAATCAAATCAAATGCTGAACTGTTTAAACTCATCAGCCTTAAACAACATTGTTTGAGGAAAATTATTTGAAAGGAATAGTTAAAAATAACAGTCAGAATATTCACATATTTATTAACTTATTGTCAAACAATTTCTTTTTATGATTATGACTAAATAACCTTCTCTATTTCTTCAAGACCCTTTCCCTTTGGCTAACCTCTCTAGAAGAGTACAGCAATCTCACAAATGCTCAGGTAAATAATCTTAGTTATTTCTTTTGTTAAGATCCCGACATGCTTGAAGTGGGGAATGGAGGGATGACCACTGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTTGCAAAAGTAAGTACAACTATGTCCTTGAGTTCTAAAGTTTTTGTTTAAATAGGGAATTTCTTGTACTATATTAGGACATATTCGAAATTCAAGTTCAGATGGAGGTTGAACATCTTATGTTCCTCTACTCGTGGGTTGGGTGTATAATTCTTGTGTGCTTCGTAAAATATATAAACCATGTTACAATCGATTCTTTTCTTTTCTTCATATATGAAGGCTCCATTGCTGATTGGATGTGACATCAGATCAATGGACAACATCACCATGAAACTGTTAAGTAACAAGGAGGTTATTGCAGTTAATCAGGGTGAGTAAAAACCAAACTGTTTCTGCTAATTCATAGAGAACTATGAAACAGATAACCAAAAATAGTAATAATTTCTCTCAATCAAATGGAAAGGTTAAAATGTTGAAGAATATTTAGATGCATCTTAGGTTGCACCCATTTTTTACCACCAAAGATGCATTCAAGCATTATTCTTGATCGTTAACTAAATATAACATTATGTGCGGCATCTAAAAGTTTTTTATCCAAGTAGATAAGCTTGGAGTCCAAGGAAAGAAGGTCCATAAATATGGAGACCTCGAGGTAATTTCTCATCATCTGATGTATTCCTAAAGGAAACTAAAAGAGAGAAAAAAAGGATAAAATTTCTACTTTACTCTTAACTTACTACACAAAACCAATGCAGGTTTGGGCAGGGCCTCTAAGTGGTAAAAGAGTCGCTGTGGTTCTATGGAACAGAGGTTTATGGCGAGCCAATATCACGGCATCTTGGTCTGATATAGGTTTGAGTTCTTCAACTACAGTTACTGCTCGAGACTTATGGGAGGTAAGATATGAATTGTCTAGTAACTAAATCCATTTTTAAAGTTTTTATGGCATTAGGTTACATAGGTCAAAAACCACTTTTTAGTTTCTAAATTTTTATCATATTATAGGTAGTGATTTAGTTCGTATACTTTTAAATTTATAAAACTTATCACTATATCTTAGTATGTAACAACTTAGTCTCTATACTCCAGATTTTGTATTGATTTAGTCTATGTACAAATGTTGTATTGGTTTAGTCTTTACAATAAATAACGATATTTATATTATGCATGTAGATTGATAAATTTATTCAAAACCAAATGTGATTATAAACTACAAAAACTAAACCGTTGCCTGACGCTTGGTTTGATGAAAATTGTTTATGAAGTGTATATATTTGTTCATCATTCAAAAGACCTGTTTTTTTCACTTCTTCTTTTTGACTAATTATTCATTCCTAACAACAGCATTCCAGCCAGGTGGTTCAACATCACCTTACTGCTCAAGTAGACTCTCATGACTGTAAGATGTTTGTTCTCACACCTCACTGA

mRNA sequence

ATGGCTCTTCCCCCATGTTCACCTTCAGTTATGCTTCTACTCTACTTCCTCCTTTTCTGGACGTTCTTGCTAGGTGGCAATGGCAATGGCAATGGCAATGGCGTTGTGGTTGCCGGAGCTTCTCGATCGACAGCTCTACGGTTTGCCACTGAGTTTGATTCTGCTTCTTCTAGGAGAGTTCTTCTTAACAATGGTCTCGCTCTAACTCCTCCGATGGGATGGAATAGTTGGAACCATTTTCAATGTAATTTAAACGAGAATTTAATCAAGGAAACAGTGGATGCAATGGTATCCACCGGACTTGCTGCATTAGGATATGGGTATATCAATTTAGATGATTGTTGGGCTGAACTTGACAGAGACTCTAAGGGTAATTTGGTTGCTAAAGCTTCAACGTTCCCTTCTGGTATTAAGGCCCTAGCAGATTATGTTCATAGAAAAGGGCTCAAGCTTGGAATTTATTCGGATGCTGGGATCCAAACTTGCAGTAAAAGGATGCCTGGTTCCCTTGGTCACGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGGGATTGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGATATCCAAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCATTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACAACATCAGATATTCAAGATAACTGGATTAGCATGATAACCATTGCTGATCAGAATGACAAATGGGCTTCTTACGCTAAACCAGGAGGATGGAATGATCCCGACATGCTTGAAGTGGGGAATGGAGGGATGACCACTGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTTGCAAAAGCTCCATTGCTGATTGGATGTGACATCAGATCAATGGACAACATCACCATGAAACTGTTAAGTAACAAGGAGGTTATTGCAGTTAATCAGGATAAGCTTGGAGTCCAAGGAAAGAAGGTCCATAAATATGGAGACCTCGAGGTTTGGGCAGGGCCTCTAAGTGGTAAAAGAGTCGCTGTGGTTCTATGGAACAGAGGTTTATGGCGAGCCAATATCACGGCATCTTGGTCTGATATAGGTTTGAGTTCTTCAACTACAGTTACTGCTCGAGACTTATGGGAGCATTCCAGCCAGGTGGTTCAACATCACCTTACTGCTCAAGTAGACTCTCATGACTGTAAGATGTTTGTTCTCACACCTCACTGA

Coding sequence (CDS)

ATGGCTCTTCCCCCATGTTCACCTTCAGTTATGCTTCTACTCTACTTCCTCCTTTTCTGGACGTTCTTGCTAGGTGGCAATGGCAATGGCAATGGCAATGGCGTTGTGGTTGCCGGAGCTTCTCGATCGACAGCTCTACGGTTTGCCACTGAGTTTGATTCTGCTTCTTCTAGGAGAGTTCTTCTTAACAATGGTCTCGCTCTAACTCCTCCGATGGGATGGAATAGTTGGAACCATTTTCAATGTAATTTAAACGAGAATTTAATCAAGGAAACAGTGGATGCAATGGTATCCACCGGACTTGCTGCATTAGGATATGGGTATATCAATTTAGATGATTGTTGGGCTGAACTTGACAGAGACTCTAAGGGTAATTTGGTTGCTAAAGCTTCAACGTTCCCTTCTGGTATTAAGGCCCTAGCAGATTATGTTCATAGAAAAGGGCTCAAGCTTGGAATTTATTCGGATGCTGGGATCCAAACTTGCAGTAAAAGGATGCCTGGTTCCCTTGGTCACGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGGGATTGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGATATCCAAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCATTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACAACATCAGATATTCAAGATAACTGGATTAGCATGATAACCATTGCTGATCAGAATGACAAATGGGCTTCTTACGCTAAACCAGGAGGATGGAATGATCCCGACATGCTTGAAGTGGGGAATGGAGGGATGACCACTGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTTGCAAAAGCTCCATTGCTGATTGGATGTGACATCAGATCAATGGACAACATCACCATGAAACTGTTAAGTAACAAGGAGGTTATTGCAGTTAATCAGGATAAGCTTGGAGTCCAAGGAAAGAAGGTCCATAAATATGGAGACCTCGAGGTTTGGGCAGGGCCTCTAAGTGGTAAAAGAGTCGCTGTGGTTCTATGGAACAGAGGTTTATGGCGAGCCAATATCACGGCATCTTGGTCTGATATAGGTTTGAGTTCTTCAACTACAGTTACTGCTCGAGACTTATGGGAGCATTCCAGCCAGGTGGTTCAACATCACCTTACTGCTCAAGTAGACTCTCATGACTGTAAGATGTTTGTTCTCACACCTCACTGA

Protein sequence

MALPPCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVLTPH*

Homology

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BLAST of Chy10G178690 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 2.7e-177
Identity = 289/373 (77.48%), Postives = 322/373 (86.33%), Query Frame = 0


Query: 50  TEFDSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYI 109
           T+       R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+T DAMVS+GL+A+GY YI
Sbjct: 20  TQIADGFQSRMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYI 79

Query: 110 NLDDCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGS 169
           N+DDCW EL RDS+G+LVAKASTFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGS
Sbjct: 80  NIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGS 139

Query: 170 LGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQED 229
           LGHEEQDAKTFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQED
Sbjct: 140 LGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQED 199

Query: 230 PATWAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTA 289
           PATWA ++GNSWRTT DIQDNW SM  IADQND+WASYA+PG WNDPDMLEVGNGGMT  
Sbjct: 200 PATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKE 259

Query: 290 EYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLE 349
           EY SHFSIWALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLE
Sbjct: 260 EYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLE 319

Query: 350 VWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSS-QVVQHHLTA 409
           VWAGPLS KRVAV+LWNRG   ANITA W++IGL+SS  V ARDLWEHS+   V+  L+A
Sbjct: 320 VWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSA 379

Query: 410 QVDSHDCKMFVLT 422
            V+ H CKM+ LT
Sbjct: 380 LVEPHACKMYTLT 392

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BLAST of Chy10G178690 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.2e-175
Identity = 291/367 (79.29%), Postives = 312/367 (85.01%), Query Frame = 0


Query: 57  SRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWA 116
           +RR LL NGL LTPPMGWNSWNHF+CNL+E LI+ET DAMVS GLAALGY YINLDDCWA
Sbjct: 11  TRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWA 70

Query: 117 ELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQD 176
           EL+RDS+GNLV K STFPSGIKALADYVH KGLKLGIYSDAG QTCSK MPGSLGHEEQD
Sbjct: 71  ELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQD 130

Query: 177 AKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVN 236
           AKTFASWG+DYLKYDNC N   SPKERYP M+KAL  SGR I FSLCEWG+EDPATWA  
Sbjct: 131 AKTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKE 190

Query: 237 VGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFS 296
           VGNSWRTT DI D+W SM + AD NDKWASYA PGGWNDPDMLEVGNGGMTT EYRSHFS
Sbjct: 191 VGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFS 250

Query: 297 IWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLS 356
           IWALAKAPLLIGCDIRSMD  T +LLSN EVIAVNQDKLGVQG KV  YGDLEVWAGPLS
Sbjct: 251 IWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLS 310

Query: 357 GKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQ-VVQHHLTAQVDSHDC 416
           GKRVAV LWNRG   A ITA WSD+GL S+  V ARDLW HS++  V+  ++A VD+HD 
Sbjct: 311 GKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSVKGQISAAVDAHDS 370

Query: 417 KMFVLTP 423
           KM+VLTP
Sbjct: 371 KMYVLTP 377

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BLAST of Chy10G178690 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 4.4e-175
Identity = 284/377 (75.33%), Postives = 318/377 (84.35%), Query Frame = 0


Query: 47  RFATEFDSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGY 106
           R + E +  + RR L  NGL  TPPMGWNSWNHF C++NEN+++ET DAMVSTGLAALGY
Sbjct: 33  RTSAEAEHYNVRRYLAENGLGQTPPMGWNSWNHFGCDINENVVRETADAMVSTGLAALGY 92

Query: 107 GYINLDDCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRM 166
            YINLDDCWAEL+RDS+GN+V  A+ FPSGIKALADYVH KGLKLG+YSDAG QTCSKRM
Sbjct: 93  QYINLDDCWAELNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVYSDAGNQTCSKRM 152

Query: 167 PGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWG 226
           PGSLGHEEQDAKTFASWG+DYLKYDNCEN G S KERYP M KAL  SGRPI FS+CEWG
Sbjct: 153 PGSLGHEEQDAKTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIFFSMCEWG 212

Query: 227 QEDPATWAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGM 286
            EDP  WA ++GNSWRTT DI+DNW SM +IAD NDKWASYA PGGWNDPDMLEVGNGGM
Sbjct: 213 WEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDMLEVGNGGM 272

Query: 287 TTAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYG 346
           TT EYRSHFSIWALAKAPLL+GCDIR+MD+ T +L+SN EVIAVNQDKLGVQGKKV    
Sbjct: 273 TTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGKKVKSTN 332

Query: 347 DLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQ-VVQHH 406
           DLEVWAGPLS  +VAV+LWNR   RA +TASWSDIGL   TTV ARDLWEHS+Q +V   
Sbjct: 333 DLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDLWEHSTQSLVSGE 392

Query: 407 LTAQVDSHDCKMFVLTP 423
           ++A++DSH CKM+VLTP
Sbjct: 393 ISAEIDSHACKMYVLTP 409

[Close]

BLAST of Chy10G178690 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 7.5e-159
Identity = 269/419 (64.20%), Postives = 308/419 (73.51%), Query Frame = 0


Query: 5   PCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRVLLNN 64
           P SP ++LLL   +  T L      GN            TA      + S  +RR    N
Sbjct: 10  PPSPRLLLLLLVAVAATLLPEAAALGN-----------FTAESRGARWRSRRARRRAFEN 69

Query: 65  GLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDRDSKG 124
           GL  TP MGWNSWNHF C +NE +I+ET DA+V+TGLA LGY Y+N+DDCWAE  RDS+G
Sbjct: 70  GLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAEYSRDSQG 129

Query: 125 NLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTFASWG 184
           N V    TFPSGIKALADYVH KGLKLGIYSDAG QTCS +MPGSL HEEQD KTFASWG
Sbjct: 130 NFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDVKTFASWG 189

Query: 185 IDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTT 244
           +DYLKYDNC + G S  ERY +M+ A++  G+ I FSLCEWG+E+PATWA  +GNSWRTT
Sbjct: 190 VDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRMGNSWRTT 249

Query: 245 SDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAP 304
            DI DNW SM + AD+ND+WA+YA PGGWNDPDMLEVGNGGM+ AEYRSHFSIWALAKAP
Sbjct: 250 GDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAKAP 309

Query: 305 LLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRVAVVL 364
           LLIGCD+RSM   T  +LSN EVIAVNQD LGVQGKKV     LEVWAGPLS  R AVVL
Sbjct: 310 LLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSNNRKAVVL 369

Query: 365 WNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVLTPH 424
           WNR  ++A ITA WS+IGL+ S  VTARDLW HSS   Q  ++A V  HDCKM+VLTP+
Sbjct: 370 WNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFAAQGQISASVAPHDCKMYVLTPN 417

[Close]

BLAST of Chy10G178690 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 1.9e-154
Identity = 253/372 (68.01%), Postives = 298/372 (80.11%), Query Frame = 0


Query: 53  DSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLD 112
           DS   RR LL NGL +TPPMGWNSWNHF CN++E +IKET DA+V+TGL+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 113 DCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGH 172
           DCWAE+ RDSKG+LV K STFPSGIKA+ADYVH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 173 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 232
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 233 WAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYR 292
           W   VGNSWRTT+DI+D W+SMI+IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 293 SHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWA 352
            HFSIWA++KAPLL+GCDIR+M   TM++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 353 GPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSS--QVVQHHLTAQV 412
           GPLSG RVA++L NRG  R +ITA W DI + +++ V ARDLWEH +  Q    +LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 413 DSHDCKMFVLTP 423
           DSH CK++VL P
Sbjct: 397 DSHACKLYVLKP 408

[Close]

BLAST of Chy10G178690 vs. ExPASy TrEMBL
Match: Q2HYY3 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G580630 PE=2 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 1.9e-242
Identity = 408/423 (96.45%), Postives = 411/423 (97.16%), Query Frame = 0


Query: 1   MALPPCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRV 60
           MALPP SPSVMLLLYFLLFWTFLLG  GNGNGNGVVVA ASRSTALRFATEFDSASSRR+
Sbjct: 1   MALPPSSPSVMLLLYFLLFWTFLLG--GNGNGNGVVVAAASRSTALRFATEFDSASSRRI 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDR 120
           LLNNGLALTPPMGWNSWNHFQCNLNENLIKET DAMVSTGLAALGY YINLDDCWAELDR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSTGLAALGYQYINLDDCWAELDR 120

Query: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTF 180
           DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGI+TCSKRMPGSLGHEEQDAKTF
Sbjct: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIRTCSKRMPGSLGHEEQDAKTF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240

Query: 241 WRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300
           WRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMT AEYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTIAEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRV 360
           AKAPLLIGCDIRSMDN TMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAG LSGKRV
Sbjct: 301 AKAPLLIGCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGLLSGKRV 360

Query: 361 AVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVL 420
           AVVLWNR LWRANITA WSDIGLSSSTTVTARDLWEHSSQVV+HHLTAQVDSHDCKMFVL
Sbjct: 361 AVVLWNRSLWRANITAYWSDIGLSSSTTVTARDLWEHSSQVVRHHLTAQVDSHDCKMFVL 420

Query: 421 TPH 424
           TPH
Sbjct: 421 TPH 421

[Close]

BLAST of Chy10G178690 vs. ExPASy TrEMBL
Match: A0A1S3BEH7 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.0e-239
Identity = 402/423 (95.04%), Postives = 409/423 (96.69%), Query Frame = 0


Query: 1   MALPPCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRV 60
           MAL P SPSVM+LLYF+L WTFLLG    GNGNGV VAGASRSTALRFA EFDS SSRRV
Sbjct: 1   MALSPSSPSVMVLLYFVLSWTFLLG----GNGNGVAVAGASRSTALRFAAEFDSVSSRRV 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDR 120
           LLNNGLALTPPMGWNSWNHFQCNLNENLIKET DAMVS+GLAALGY YINLDDCWAELDR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDR 120

Query: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTF 180
           DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSLG+EEQDAKTF
Sbjct: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240

Query: 241 WRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300
           WRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRV 360
           AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKV+KYGDLEVWAGPLSGKRV
Sbjct: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRV 360

Query: 361 AVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVL 420
           AVVLWNRGLWRANITASWSDIGL SSTTVTARDLW+HSSQVVQHHLTAQVDSHDCKMFVL
Sbjct: 361 AVVLWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVL 419

Query: 421 TPH 424
           TPH
Sbjct: 421 TPH 419

[Close]

BLAST of Chy10G178690 vs. ExPASy TrEMBL
Match: A0A5A7SVZ2 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003280 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 4.9e-238
Identity = 402/424 (94.81%), Postives = 409/424 (96.46%), Query Frame = 0


Query: 1   MALPPCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRV 60
           MAL P SPSVM+LLYF+L WTFLLG    GNGNGV VAGASRSTALRFA EFDS SSRRV
Sbjct: 1   MALSPSSPSVMVLLYFVLSWTFLLG----GNGNGVAVAGASRSTALRFAAEFDSVSSRRV 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDR 120
           LLNNGLALTPPMGWNSWNHFQCNLNENLIKET DAMVS+GLAALGY YINLDDCWAELDR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDR 120

Query: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTF 180
           DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSLG+EEQDAKTF
Sbjct: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240

Query: 241 WRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300
           WRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQ-DKLGVQGKKVHKYGDLEVWAGPLSGKR 360
           AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQ DKLGVQGKKV+KYGDLEVWAGPLSGKR
Sbjct: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKR 360

Query: 361 VAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFV 420
           VAVVLWNRGLWRANITASWSDIGL SSTTVTARDLW+HSSQVVQHHLTAQVDSHDCKMFV
Sbjct: 361 VAVVLWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFV 420

Query: 421 LTPH 424
           LTPH
Sbjct: 421 LTPH 420

[Close]

BLAST of Chy10G178690 vs. ExPASy TrEMBL
Match: A0A6J1HTR9 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.2e-221
Identity = 371/415 (89.40%), Postives = 385/415 (92.77%), Query Frame = 0


Query: 9   SVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRVLLNNGLAL 68
           S+MLLLY +LFWTF    NGNG      +AGA+RS  LRFATEFD  S RRVLLNNGLAL
Sbjct: 7   SIMLLLYSVLFWTFFFSSNGNGG----AMAGATRSRPLRFATEFDYDSPRRVLLNNGLAL 66

Query: 69  TPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDRDSKGNLVA 128
           TPPMGWNSWNHFQCN+NENLIKET DAMVS+GLAALGY YINLDDCWAEL+RD+KGNLV 
Sbjct: 67  TPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGNLVP 126

Query: 129 KASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTFASWGIDYL 188
           K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSL HEEQDAKTFASWGIDYL
Sbjct: 127 KSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLDHEEQDAKTFASWGIDYL 186

Query: 189 KYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQ 248
           KYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQ
Sbjct: 187 KYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQ 246

Query: 249 DNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIG 308
           DNW SM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIG
Sbjct: 247 DNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIG 306

Query: 309 CDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRVAVVLWNRG 368
           CDIRSMDNIT+KLLSNKEVIAVNQDKLGVQGKKV+K GDLEVWAG LSGKRVAVVLWNRG
Sbjct: 307 CDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKLGDLEVWAGTLSGKRVAVVLWNRG 366

Query: 369 LWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVLTPH 424
            +RA ITASWS IGLS STTVTARDLWEHSSQVVQH LTAQVD HDCKM+VLTPH
Sbjct: 367 FYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQLTAQVDPHDCKMYVLTPH 417

[Close]

BLAST of Chy10G178690 vs. ExPASy TrEMBL
Match: A0A6J1FZL3 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.1e-220
Identity = 369/415 (88.92%), Postives = 384/415 (92.53%), Query Frame = 0


Query: 9   SVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRVLLNNGLAL 68
           S+ML LY +LFWTFL   NGNG      +AGA+RS  LRFA EFD  S RRVLLNNGLAL
Sbjct: 7   SIMLFLYSVLFWTFLFSSNGNGG----AMAGATRSRPLRFAAEFDYDSPRRVLLNNGLAL 66

Query: 69  TPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDRDSKGNLVA 128
           TPPMGWNSWNHFQCN+NENLIKET DAMVS+GLAALGY YINLDDCWAEL+RD+KGNLV 
Sbjct: 67  TPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGNLVP 126

Query: 129 KASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTFASWGIDYL 188
           K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSLGHEEQDAKTFASWGIDYL
Sbjct: 127 KSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYL 186

Query: 189 KYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQ 248
           KYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQ
Sbjct: 187 KYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQ 246

Query: 249 DNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIG 308
           DNW SM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIG
Sbjct: 247 DNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIG 306

Query: 309 CDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRVAVVLWNRG 368
           CDIRSMDNIT+KLLSNK VIAVNQDKLGVQGKKV+K GDLEVWAG LSGKRVAVVLWNRG
Sbjct: 307 CDIRSMDNITLKLLSNKLVIAVNQDKLGVQGKKVYKLGDLEVWAGALSGKRVAVVLWNRG 366

Query: 369 LWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVLTPH 424
            +RA ITASWS IGLS STTVTARDLWEHSSQVVQH +TAQVD HDCKM+VLTPH
Sbjct: 367 FYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYVLTPH 417

[Close]

BLAST of Chy10G178690 vs. NCBI nr
Match: NP_001267530.1 (alpha-galactosidase-like precursor [Cucumis sativus] >ABC88435.1 acid alpha galactosidase 2 [Cucumis sativus] >KGN51578.1 hypothetical protein Csa_008622 [Cucumis sativus])

HSP 1 Score: 841 bits (2172), Expect = 1.65e-307
Identity = 408/423 (96.45%), Postives = 411/423 (97.16%), Query Frame = 0


Query: 1   MALPPCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRV 60
           MALPP SPSVMLLLYFLLFWTFLLGGNGNGNG  VVVA ASRSTALRFATEFDSASSRR+
Sbjct: 1   MALPPSSPSVMLLLYFLLFWTFLLGGNGNGNG--VVVAAASRSTALRFATEFDSASSRRI 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDR 120
           LLNNGLALTPPMGWNSWNHFQCNLNENLIKET DAMVSTGLAALGY YINLDDCWAELDR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSTGLAALGYQYINLDDCWAELDR 120

Query: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTF 180
           DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGI+TCSKRMPGSLGHEEQDAKTF
Sbjct: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIRTCSKRMPGSLGHEEQDAKTF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240

Query: 241 WRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300
           WRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMT AEYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTIAEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRV 360
           AKAPLLIGCDIRSMDN TMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAG LSGKRV
Sbjct: 301 AKAPLLIGCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGLLSGKRV 360

Query: 361 AVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVL 420
           AVVLWNR LWRANITA WSDIGLSSSTTVTARDLWEHSSQVV+HHLTAQVDSHDCKMFVL
Sbjct: 361 AVVLWNRSLWRANITAYWSDIGLSSSTTVTARDLWEHSSQVVRHHLTAQVDSHDCKMFVL 420

Query: 421 TPH 423
           TPH
Sbjct: 421 TPH 421

[Close]

BLAST of Chy10G178690 vs. NCBI nr
Match: XP_008445911.1 (PREDICTED: alpha-galactosidase [Cucumis melo])

HSP 1 Score: 831 bits (2146), Expect = 1.41e-303
Identity = 402/423 (95.04%), Postives = 409/423 (96.69%), Query Frame = 0


Query: 1   MALPPCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRV 60
           MAL P SPSVM+LLYF+L WTFLLGGNGNG    V VAGASRSTALRFA EFDS SSRRV
Sbjct: 1   MALSPSSPSVMVLLYFVLSWTFLLGGNGNG----VAVAGASRSTALRFAAEFDSVSSRRV 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDR 120
           LLNNGLALTPPMGWNSWNHFQCNLNENLIKET DAMVS+GLAALGY YINLDDCWAELDR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDR 120

Query: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTF 180
           DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSLG+EEQDAKTF
Sbjct: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240

Query: 241 WRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300
           WRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGKRV 360
           AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKV+KYGDLEVWAGPLSGKRV
Sbjct: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRV 360

Query: 361 AVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFVL 420
           AVVLWNRGLWRANITASWSDIGL SSTTVTARDLW+HSSQVVQHHLTAQVDSHDCKMFVL
Sbjct: 361 AVVLWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVL 419

Query: 421 TPH 423
           TPH
Sbjct: 421 TPH 419

[Close]

BLAST of Chy10G178690 vs. NCBI nr
Match: KAA0034096.1 (alpha-galactosidase [Cucumis melo var. makuwa] >TYK15823.1 alpha-galactosidase [Cucumis melo var. makuwa])

HSP 1 Score: 826 bits (2134), Expect = 9.87e-302
Identity = 402/424 (94.81%), Postives = 409/424 (96.46%), Query Frame = 0


Query: 1   MALPPCSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSASSRRV 60
           MAL P SPSVM+LLYF+L WTFLLGGNGNG    V VAGASRSTALRFA EFDS SSRRV
Sbjct: 1   MALSPSSPSVMVLLYFVLSWTFLLGGNGNG----VAVAGASRSTALRFAAEFDSVSSRRV 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAELDR 120
           LLNNGLALTPPMGWNSWNHFQCNLNENLIKET DAMVS+GLAALGY YINLDDCWAELDR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDR 120

Query: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAKTF 180
           DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSLG+EEQDAKTF
Sbjct: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240

Query: 241 WRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300
           WRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQ-DKLGVQGKKVHKYGDLEVWAGPLSGKR 360
           AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQ DKLGVQGKKV+KYGDLEVWAGPLSGKR
Sbjct: 301 AKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKR 360

Query: 361 VAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMFV 420
           VAVVLWNRGLWRANITASWSDIGL SSTTVTARDLW+HSSQVVQHHLTAQVDSHDCKMFV
Sbjct: 361 VAVVLWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFV 420

Query: 421 LTPH 423
           LTPH
Sbjct: 421 LTPH 420

[Close]

BLAST of Chy10G178690 vs. NCBI nr
Match: XP_038892679.1 (alpha-galactosidase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 786 bits (2031), Expect = 5.11e-286
Identity = 379/427 (88.76%), Postives = 396/427 (92.74%), Query Frame = 0


Query: 1   MALPP----CSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSAS 60
           MA PP      PS + LL  +LFW FLLG NG        VAGA RS +LRFA  FDS S
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGSNGG------AVAGAIRSRSLRFAAGFDSVS 60

Query: 61  SRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWA 120
           +RRVLLNNGLALTPPMGWNSWNHFQCN+NENLIKET DAMVS+GLA+LGY YINLDDCWA
Sbjct: 61  TRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCWA 120

Query: 121 ELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQD 180
           EL+RDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSLGHE+QD
Sbjct: 121 ELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEQQD 180

Query: 181 AKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVN 240
           A+TFASWGIDYLKYDNCENTGTSPKERYPKM+KALQQSGRPILFSLCEWGQEDPATWA+N
Sbjct: 181 AETFASWGIDYLKYDNCENTGTSPKERYPKMSKALQQSGRPILFSLCEWGQEDPATWAIN 240

Query: 241 VGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFS 300
           VGNSWRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFS
Sbjct: 241 VGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFS 300

Query: 301 IWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLS 360
           IWALAKAPLLIGCDIRSMDNI +KLLSNKEVIAVNQDKLGVQGKKV+KYGDLEVWAGPLS
Sbjct: 301 IWALAKAPLLIGCDIRSMDNIALKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLS 360

Query: 361 GKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDCK 420
           GKRVAVVLWNRGLWRANITASWSDIGLS+STTVTARDLWEHSSQVVQHHLTAQVDSHDCK
Sbjct: 361 GKRVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDCK 420

Query: 421 MFVLTPH 423
           M+VLTPH
Sbjct: 421 MYVLTPH 421

[Close]

BLAST of Chy10G178690 vs. NCBI nr
Match: XP_038892678.1 (alpha-galactosidase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 782 bits (2019), Expect = 3.57e-284
Identity = 379/428 (88.55%), Postives = 396/428 (92.52%), Query Frame = 0


Query: 1   MALPP----CSPSVMLLLYFLLFWTFLLGGNGNGNGNGVVVAGASRSTALRFATEFDSAS 60
           MA PP      PS + LL  +LFW FLLG NG        VAGA RS +LRFA  FDS S
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGSNGG------AVAGAIRSRSLRFAAGFDSVS 60

Query: 61  SRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWA 120
           +RRVLLNNGLALTPPMGWNSWNHFQCN+NENLIKET DAMVS+GLA+LGY YINLDDCWA
Sbjct: 61  TRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCWA 120

Query: 121 ELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQD 180
           EL+RDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSK+MPGSLGHE+QD
Sbjct: 121 ELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEQQD 180

Query: 181 AKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVN 240
           A+TFASWGIDYLKYDNCENTGTSPKERYPKM+KALQQSGRPILFSLCEWGQEDPATWA+N
Sbjct: 181 AETFASWGIDYLKYDNCENTGTSPKERYPKMSKALQQSGRPILFSLCEWGQEDPATWAIN 240

Query: 241 VGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFS 300
           VGNSWRTTSDIQDNWISM TIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFS
Sbjct: 241 VGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFS 300

Query: 301 IWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQ-DKLGVQGKKVHKYGDLEVWAGPL 360
           IWALAKAPLLIGCDIRSMDNI +KLLSNKEVIAVNQ DKLGVQGKKV+KYGDLEVWAGPL
Sbjct: 301 IWALAKAPLLIGCDIRSMDNIALKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPL 360

Query: 361 SGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSSQVVQHHLTAQVDSHDC 420
           SGKRVAVVLWNRGLWRANITASWSDIGLS+STTVTARDLWEHSSQVVQHHLTAQVDSHDC
Sbjct: 361 SGKRVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDC 420

Query: 421 KMFVLTPH 423
           KM+VLTPH
Sbjct: 421 KMYVLTPH 422

[Close]

BLAST of Chy10G178690 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 622.9 bits (1605), Expect = 2.0e-178
Identity = 289/373 (77.48%), Postives = 322/373 (86.33%), Query Frame = 0


Query: 50  TEFDSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYI 109
           T+       R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+T DAMVS+GL+A+GY YI
Sbjct: 20  TQIADGFQSRMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYI 79

Query: 110 NLDDCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGS 169
           N+DDCW EL RDS+G+LVAKASTFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGS
Sbjct: 80  NIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGS 139

Query: 170 LGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQED 229
           LGHEEQDAKTFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQED
Sbjct: 140 LGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQED 199

Query: 230 PATWAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTA 289
           PATWA ++GNSWRTT DIQDNW SM  IADQND+WASYA+PG WNDPDMLEVGNGGMT  
Sbjct: 200 PATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKE 259

Query: 290 EYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLE 349
           EY SHFSIWALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLE
Sbjct: 260 EYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLE 319

Query: 350 VWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSS-QVVQHHLTA 409
           VWAGPLS KRVAV+LWNRG   ANITA W++IGL+SS  V ARDLWEHS+   V+  L+A
Sbjct: 320 VWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSA 379

Query: 410 QVDSHDCKMFVLT 422
            V+ H CKM+ LT
Sbjct: 380 LVEPHACKMYTLT 392

[Close]

BLAST of Chy10G178690 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 622.1 bits (1603), Expect = 3.3e-178
Identity = 288/364 (79.12%), Postives = 320/364 (87.91%), Query Frame = 0


Query: 59  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLDDCWAEL 118
           R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+T DAMVS+GL+A+GY YIN+DDCW EL
Sbjct: 3   RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 62

Query: 119 DRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGHEEQDAK 178
            RDS+G+LVAKASTFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 63  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 122

Query: 179 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 238
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 123 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 182

Query: 239 NSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 298
           NSWRTT DIQDNW SM  IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIW
Sbjct: 183 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 242

Query: 299 ALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGPLSGK 358
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 243 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 302

Query: 359 RVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSS-QVVQHHLTAQVDSHDCKM 418
           RVAV+LWNRG   ANITA W++IGL+SS  V ARDLWEHS+   V+  L+A V+ H CKM
Sbjct: 303 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 362

Query: 419 FVLT 422
           + LT
Sbjct: 363 YTLT 366

[Close]

BLAST of Chy10G178690 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 547.0 bits (1408), Expect = 1.4e-155
Identity = 253/372 (68.01%), Postives = 298/372 (80.11%), Query Frame = 0


Query: 53  DSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYGYINLD 112
           DS   RR LL NGL +TPPMGWNSWNHF CN++E +IKET DA+V+TGL+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 113 DCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMPGSLGH 172
           DCWAE+ RDSKG+LV K STFPSGIKA+ADYVH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 173 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 232
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 233 WAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYR 292
           W   VGNSWRTT+DI+D W+SMI+IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 293 SHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWA 352
            HFSIWA++KAPLL+GCDIR+M   TM++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 353 GPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHSS--QVVQHHLTAQV 412
           GPLSG RVA++L NRG  R +ITA W DI + +++ V ARDLWEH +  Q    +LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 413 DSHDCKMFVLTP 423
           DSH CK++VL P
Sbjct: 397 DSHACKLYVLKP 408

[Close]

BLAST of Chy10G178690 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 515.4 bits (1326), Expect = 4.4e-146
Identity = 243/380 (63.95%), Postives = 285/380 (75.00%), Query Frame = 0


Query: 48  FATEFDSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYG 107
           F + +D++   R+ LNNGLA TP MGWNSWN F CN+NE +IKET DA+VS+GLA LGY 
Sbjct: 51  FNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYI 110

Query: 108 YINLDDCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMP 167
           ++N+DDCW+ L RDS+G LV    TFPSGIK LADYVH KGLKLGIYSDAG+ TC    P
Sbjct: 111 HVNIDDCWSNLLRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTCEVH-P 170

Query: 168 GSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQ 227
           GSL HE  DA  FASWG+DYLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG 
Sbjct: 171 GSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGV 230

Query: 228 EDPATWAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMT 287
           +DPA WA  VGNSWRTT DI D W SM TIAD N+KWA+YA PGGWNDPDMLE+GNGGMT
Sbjct: 231 DDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMT 290

Query: 288 TAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGD 347
             EYR HFSIWAL KAPLLIGCD+R+M   T+++LSNKE+IAVNQD LGVQG+K+   G+
Sbjct: 291 YEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGE 350

Query: 348 ---LEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHS--SQVV 407
               +VW+GPLSG R+ V LWNR    A ITASW  IGL S+ +V+ RDLW+H   ++  
Sbjct: 351 NDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENT 410

Query: 408 QHHLTAQVDSHDCKMFVLTP 423
                AQVD+HDC M+VLTP
Sbjct: 411 SGSFEAQVDAHDCHMYVLTP 429

[Close]

BLAST of Chy10G178690 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 4.7e-132
Identity = 228/380 (60.00%), Postives = 268/380 (70.53%), Query Frame = 0


Query: 48  FATEFDSASSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETVDAMVSTGLAALGYG 107
           F + +D++   R+ LNNGLA TP MGWNSWN F CN+NE +IKET DA+VS+GLA LGY 
Sbjct: 51  FNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYI 110

Query: 108 YINLDDCWAELDRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKRMP 167
           ++N+                        GIK LADYVH KGLKLGIYSDAG+ TC    P
Sbjct: 111 HVNI------------------------GIKLLADYVHSKGLKLGIYSDAGVFTCEVH-P 170

Query: 168 GSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQ 227
           GSL HE  DA  FASWG+DYLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG 
Sbjct: 171 GSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGV 230

Query: 228 EDPATWAVNVGNSWRTTSDIQDNWISMITIADQNDKWASYAKPGGWNDPDMLEVGNGGMT 287
           +DPA WA  VGNSWRTT DI D W SM TIAD N+KWA+YA PGGWNDPDMLE+GNGGMT
Sbjct: 231 DDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMT 290

Query: 288 TAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVHKYGD 347
             EYR HFSIWAL KAPLLIGCD+R+M   T+++LSNKE+IAVNQD LGVQG+K+   G+
Sbjct: 291 YEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGE 350

Query: 348 ---LEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSSSTTVTARDLWEHS--SQVV 407
               +VW+GPLSG R+ V LWNR    A ITASW  IGL S+ +V+ RDLW+H   ++  
Sbjct: 351 NDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENT 405

Query: 408 QHHLTAQVDSHDCKMFVLTP 423
                AQVD+HDC M+VLTP
Sbjct: 411 SGSFEAQVDAHDCHMYVLTP 405

The following BLAST results are available for this feature:

BLAST of Chy10G178690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RX86	2.7e-177	77.48	Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1	[more]
Q42656	1.2e-175	79.29	Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1	[more]
P14749	4.4e-175	75.33	Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1	[more]
Q9FXT4	7.5e-159	64.20	Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=...	[more]
Q9FT97	1.9e-154	68.01	Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1	[more]

BLAST of Chy10G178690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q2HYY3	1.9e-242	96.45	Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G580630 PE=2 SV=1	[more]
A0A1S3BEH7	2.0e-239	95.04	Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1	[more]
A0A5A7SVZ2	4.9e-238	94.81	Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0...	[more]
A0A6J1HTR9	7.2e-221	89.40	Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1	[more]
A0A6J1FZL3	6.1e-220	88.92	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1	[more]

BLAST of Chy10G178690 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001267530.1	1.65e-307	96.45	alpha-galactosidase-like precursor [Cucumis sativus] >ABC88435.1 acid alpha gala...	[more]
XP_008445911.1	1.41e-303	95.04	PREDICTED: alpha-galactosidase [Cucumis melo]	[more]
KAA0034096.1	9.87e-302	94.81	alpha-galactosidase [Cucumis melo var. makuwa] >TYK15823.1 alpha-galactosidase [...	[more]
XP_038892679.1	5.11e-286	88.76	alpha-galactosidase-like isoform X2 [Benincasa hispida]	[more]
XP_038892678.1	3.57e-284	88.55	alpha-galactosidase-like isoform X1 [Benincasa hispida]	[more]

BLAST of Chy10G178690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08370.1	2.0e-178	77.48	alpha-galactosidase 2	[more]
AT5G08370.2	3.3e-178	79.12	alpha-galactosidase 2	[more]
AT5G08380.1	1.4e-155	68.01	alpha-galactosidase 1	[more]
AT3G56310.1	4.4e-146	63.95	Melibiase family protein	[more]
AT3G56310.2	4.7e-132	60.00	Melibiase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002241	Glycoside hydrolase, family 27	PRINTS	PR00740	GLHYDRLASE27	coord: 176..193 score: 76.39 coord: 141..162 score: 62.5 coord: 203..221 score: 52.37 coord: 64..83 score: 66.5 coord: 289..310 score: 55.91 coord: 268..287 score: 69.5 coord: 100..115 score: 68.75
IPR002241	Glycoside hydrolase, family 27	PFAM	PF16499	Melibiase_2	coord: 69..333 e-value: 1.7E-79 score: 266.9
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 51..422
IPR002241	Glycoside hydrolase, family 27	CDD	cd14792	GH27	coord: 70..333 e-value: 4.48742E-158 score: 445.076
IPR041233	Alpha galactosidase, C-terminal beta sandwich domain	PFAM	PF17801	Melibiase_C	coord: 346..420 e-value: 8.5E-20 score: 70.6
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 336..423 e-value: 1.3E-30 score: 107.1
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 62..335 e-value: 1.1E-137 score: 460.1
None	No IPR available	PANTHER	PTHR11452:SF33	ALPHA-GALACTOSIDASE 2	coord: 51..422
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 328..421
IPR000111	Glycoside hydrolase family 27/36, conserved site	PROSITE	PS00512	ALPHA_GALACTOSIDASE	coord: 105..121
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 60..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G178690.1	Chy10G178690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0009965	leaf morphogenesis
biological_process	GO:0009911	positive regulation of flower development
biological_process	GO:0009620	response to fungus
cellular_component	GO:0048046	apoplast
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009505	plant-type cell wall
molecular_function	GO:0052692	raffinose alpha-galactosidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds