Chy10G178550 (gene) Cucumber (hystrix) v1

Overview
NameChy10G178550
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionTMV resistance protein N-like
LocationchrH10: 13091942 .. 13093115 (+)
RNA-Seq ExpressionChy10G178550
SyntenyChy10G178550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGACGGCATGTGCTCTCAATCCAGATTATGGAATTACAGCTCTCACTGATCTTTCTCTTGTTTCTATTGAAAATGGTAAGGTGCAAATGCATGATTTAATACAACAAATGGGTCATACTATTGTTCGTGGTGAATCATTTGACCTTGGAAGAAGGAGTAGGTTGTGGGTAGCAAACGAAGTTATTAGTATTTTGAAAAAGAATTCAGTGAGTAGCCTGACACAGTGTTTTCATTTTAGTTAAGTTAATGAGAAATGTTAATCACTTGATTTACAACTGTGCAGGAAACAGATGCAGTTAAAGCCATAAAGCTGGACTTATCCAACGACTTATCACCGACATCGATTGATGCACAAGCATTTAGAAATATTAAAAATCTTAAATTGCTTATCCTTCGAAATATAACATTTTCTTCAGATATGTTTGAGTATAGTCTTAATAAGTTCAAGTGGGTTTTATCTAGATTTGGAATTAAATTTTCATCTTTGCCTGCAAATTTTTTGGTGAAAGATGGGCTTGTTGGACTAGACATGCAGCATTCTTGCATCAAACATTTGGGGAATAGATTTGAGGTAAATTAAAGTTGGTAGTTTGTGAGCCAGATTTTTCCAGAAATATTATTTATTATGACTTTCTTTTTCCATACTGTGTTTAGAATTGTGAAGGGTTAAAGTATGTTGATCTCAGTTACTGTGAGCTCTTGGAGGACATTCCCGACTTATCTGCTGCAGTAAACCTTGAGTATTTGTATCTAAGAGGGTGCGTAAGTTTAAAAATGATTCACGAGTCTGTTGCTTCTCTTAACAAGTTGGTAACCTTAGACCTTGAGGGTTGTGTTAATCTTGAAAAGCTTCCTAGCTACATCAGGTTAAAATATCTTGATTCGTTCTGTCTTTCGGGCTGCCGTAAACTTCAACGAGTTCCAGAATTTGATGAAAACATGAAATCTTTAACAAGGATGATTTTAGACTATACTGCCATAGAGGAGTTACCTTCATCGATTGAACATCTTGCTAAGCTTGAGTTTTTAAGCCTTATAGGTTGTGCAAACCTCTTAGCTCTTCCAAGTGAATTTTTTTTGTTAGAGTCTTGTGCAACTCGACATTCCTTGTCCAATAACTACCCAACAAATATTCCTTCTTCTCTCAAAGCTGACTGTATTGGACCTTAA

mRNA sequence

ATGTTGACGGCATGTGCTCTCAATCCAGATTATGGAATTACAGCTCTCACTGATCTTTCTCTTGTTTCTATTGAAAATGGTAAGGTGCAAATGCATGATTTAATACAACAAATGGGTCATACTATTGTTCGTGGTGAATCATTTGACCTTGGAAGAAGGAGTAGGTTGTGGGTAGCAAACGAAGTTATTAGTATTTTGAAAAAGAATTCAGAAACAGATGCAGTTAAAGCCATAAAGCTGGACTTATCCAACGACTTATCACCGACATCGATTGATGCACAAGCATTTAGAAATATTAAAAATCTTAAATTGCTTATCCTTCGAAATATAACATTTTCTTCAGATATGTTTGAGTATAGTCTTAATAAGTTCAAGTGGGTTTTATCTAGATTTGGAATTAAATTTTCATCTTTGCCTGCAAATTTTTTGGTGAAAGATGGGCTTGTTGGACTAGACATGCAGCATTCTTGCATCAAACATTTGGGGAATAGATTTGAGAATTGTGAAGGGTTAAAGTATGTTGATCTCAGTTACTGTGAGCTCTTGGAGGACATTCCCGACTTATCTGCTGCAGTAAACCTTGAGTATTTGTATCTAAGAGGGTGCGTAAGTTTAAAAATGATTCACGAGTCTGTTGCTTCTCTTAACAAGTTGGTAACCTTAGACCTTGAGGGTTGTGTTAATCTTGAAAAGCTTCCTAGCTACATCAGGTTAAAATATCTTGATTCGTTCTGTCTTTCGGGCTGCCGTAAACTTCAACGAGTTCCAGAATTTGATGAAAACATGAAATCTTTAACAAGGATGATTTTAGACTATACTGCCATAGAGGAGTTACCTTCATCGATTGAACATCTTGCTAAGCTTGAGTTTTTAAGCCTTATAGGTTGTGCAAACCTCTTAGCTCTTCCAAGTGAATTTTTTTTGTTAGAGTCTTGTGCAACTCGACATTCCTTGTCCAATAACTACCCAACAAATATTCCTTCTTCTCTCAAAGCTGACTGTATTGGACCTTAA

Coding sequence (CDS)

ATGTTGACGGCATGTGCTCTCAATCCAGATTATGGAATTACAGCTCTCACTGATCTTTCTCTTGTTTCTATTGAAAATGGTAAGGTGCAAATGCATGATTTAATACAACAAATGGGTCATACTATTGTTCGTGGTGAATCATTTGACCTTGGAAGAAGGAGTAGGTTGTGGGTAGCAAACGAAGTTATTAGTATTTTGAAAAAGAATTCAGAAACAGATGCAGTTAAAGCCATAAAGCTGGACTTATCCAACGACTTATCACCGACATCGATTGATGCACAAGCATTTAGAAATATTAAAAATCTTAAATTGCTTATCCTTCGAAATATAACATTTTCTTCAGATATGTTTGAGTATAGTCTTAATAAGTTCAAGTGGGTTTTATCTAGATTTGGAATTAAATTTTCATCTTTGCCTGCAAATTTTTTGGTGAAAGATGGGCTTGTTGGACTAGACATGCAGCATTCTTGCATCAAACATTTGGGGAATAGATTTGAGAATTGTGAAGGGTTAAAGTATGTTGATCTCAGTTACTGTGAGCTCTTGGAGGACATTCCCGACTTATCTGCTGCAGTAAACCTTGAGTATTTGTATCTAAGAGGGTGCGTAAGTTTAAAAATGATTCACGAGTCTGTTGCTTCTCTTAACAAGTTGGTAACCTTAGACCTTGAGGGTTGTGTTAATCTTGAAAAGCTTCCTAGCTACATCAGGTTAAAATATCTTGATTCGTTCTGTCTTTCGGGCTGCCGTAAACTTCAACGAGTTCCAGAATTTGATGAAAACATGAAATCTTTAACAAGGATGATTTTAGACTATACTGCCATAGAGGAGTTACCTTCATCGATTGAACATCTTGCTAAGCTTGAGTTTTTAAGCCTTATAGGTTGTGCAAACCTCTTAGCTCTTCCAAGTGAATTTTTTTTGTTAGAGTCTTGTGCAACTCGACATTCCTTGTCCAATAACTACCCAACAAATATTCCTTCTTCTCTCAAAGCTGACTGTATTGGACCTTAA

Protein sequence

MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVANEVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYSLNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNLEKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLIGCANLLALPSEFFLLESCATRHSLSNNYPTNIPSSLKADCIGP*
Homology
BLAST of Chy10G178550 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.5e-37
Identity = 126/361 (34.90%), Postives = 182/361 (50.42%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESF--DLGRRSRLWV 60
           +L AC  + + GI  L D  L++++  +++MHDLIQQMG  IVR E F  +  + SRLW 
Sbjct: 468 ILDACDFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVR-EKFPDEPNKWSRLWD 527

Query: 61  ANEVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILR---NITFSSD 120
             +    L        V+ I LDLS  L     ++ AF  +  L+LL ++   +I F  +
Sbjct: 528 TCDFERALTAYKGIKRVETISLDLSK-LKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPE 587

Query: 121 MFE-------YSL---NKFKWVLSRFGIKFSSLPANFLVKDG--------------LVGL 180
             +       Y +   N  K  L R G KF S    +L  DG              LV L
Sbjct: 588 YIDADDKVELYDVVMKNASKMRLGR-GFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVEL 647

Query: 181 DMQHSCIKHLGNRFENCEGLKYVDLSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHES 240
            ++ S IK L    ++ E LK +DLSY   L  + + S+  NLE L+LRGCVSL  IH S
Sbjct: 648 HLKCSNIKQLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPS 707

Query: 241 VASLNKLVTLDLEGCVNLEKLPSYI-RLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMIL 300
           V ++ KL TL L+ C  L+ LP  I  L+ L+   L+ C K ++ PE   NMKSLT + L
Sbjct: 708 VGNMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDL 767

Query: 301 DYTAIEELPSSIEHLAKLEFLSLIGCANLLALPSEFFLLESCATRHSLSNNYPTNIPSSL 332
             TAI++LP SI  L  L++L L  C+     P +   ++S      L N    ++P S+
Sbjct: 768 QNTAIKDLPDSIGDLESLKYLDLSDCSKFEKFPEKGGNMKS-LRELDLRNTAIKDLPDSI 824

BLAST of Chy10G178550 vs. ExPASy Swiss-Prot
Match: Q9SZ67 (Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 9.9e-37
Identity = 121/335 (36.12%), Postives = 175/335 (52.24%), Query Frame = 0

Query: 1    MLTACALNPDYGITALTDLSLVSI-ENGKVQMHDLIQQMGHTIVRGESFDL-GRRSRLWV 60
            +L  C  +   G   L D SL++I ++  V M   IQ  G  IVR ES D  G RSRLW 
Sbjct: 1077 LLDGCGFSAHVGFRGLVDKSLLTISQHNLVDMLSFIQATGREIVRQESADRPGDRSRLWN 1136

Query: 61   ANEVISILKKNSETDAVKAIKLDLSN---DLSPTSIDAQAFRNIKNLKLLIL-------- 120
            A+ +  +   ++ T A++ I LD+ N   D +P       F  + NL+LL L        
Sbjct: 1137 ADYIRHVFINDTGTSAIEGIFLDMLNLKFDANP-----NVFEKMCNLRLLKLYCSKAEEK 1196

Query: 121  RNITFSSDMFEYSLNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHL--GNRF 180
              ++F   + EY  +K + +   +    SSLP +F   + LV L++  SC K L  G + 
Sbjct: 1197 HGVSFPQGL-EYLPSKLRLLHWEY-YPLSSLPKSF-NPENLVELNLPSSCAKKLWKGKKA 1256

Query: 181  ENC------EGLKYVDLSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLV 240
              C      E LK + LSY + L  IP LS+A NLE++ L GC SL  + +S++ L KLV
Sbjct: 1257 RFCTTNSSLEKLKKMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSISYLKKLV 1316

Query: 241  TLDLEGCVNLEKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELP 300
             L+L+GC  LE +PS + L+ L+   LSGC KL   PE   N+K L    +  T I+E+P
Sbjct: 1317 FLNLKGCSKLENIPSMVDLESLEVLNLSGCSKLGNFPEISPNVKEL---YMGGTMIQEIP 1376

Query: 301  SSIEHLAKLEFLSLIGCANLLALPSEFFLLESCAT 315
            SSI++L  LE L L    +L  LP+  + L+   T
Sbjct: 1377 SSIKNLVLLEKLDLENSRHLKNLPTSIYKLKHLET 1400

BLAST of Chy10G178550 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.2e-36
Identity = 127/368 (34.51%), Postives = 175/368 (47.55%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDL--GRRSRLWV 60
           +L  C    + GI+ L DL L+++   ++ MHDLIQQMG  IVR E+F +   + SRLW 
Sbjct: 463 ILDGCDFPAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVR-ENFPVEPNKWSRLWD 522

Query: 61  ANEVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMF- 120
             +    L  +    +V+ + LDLS  L     ++  F  +  L+LL    +  SSD+  
Sbjct: 523 PCDFERALTADEGIKSVETMSLDLSK-LKRVCSNSNVFAKMTKLRLL---KVYSSSDIDS 582

Query: 121 --------------------------------EYSLNKFKWVLSRFGIKFSSLPANFLVK 180
                                            Y L   +W     G    SLP NF   
Sbjct: 583 AHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYELRYLRW----DGYPLDSLPLNF--- 642

Query: 181 DG--LVGLDMQHSCIKHLGNRFENCEGLKYVDLSYCELLEDIPDLSAAVNLEYLYLRGCV 240
           DG  LV L ++ S IK L    ++ E LK +DLSY   L  + + S+  NLE L L GCV
Sbjct: 643 DGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCV 702

Query: 241 SLKMIHESVASLNKLVTLDLEGCVNLEKLPSYI-RLKYLDSFCLSGCRKLQRVPEFDENM 300
           SL  IH SV ++ KL TL L  C  L+ LP  I  L+ L+S  LS C K ++ PE   NM
Sbjct: 703 SLIDIHPSVGNMKKLTTLSLRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNM 762

Query: 301 KSLTRMILDYTAIEELPSSIEHLAKLEFLSLIGCANLLALPSEFFLLESCATRHSLSNNY 331
           KSLT + L  TAI++LP SI  L  LE L L  C+     P +   ++S  T   L N  
Sbjct: 763 KSLTELDLKNTAIKDLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKS-LTELDLKNTA 817

BLAST of Chy10G178550 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 7.9e-34
Identity = 100/309 (32.36%), Postives = 153/309 (49.51%), Query Frame = 0

Query: 4   ACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVANEVI 63
           A   +P  G+  L + SL+ I   K+QMHDL+Q+MG  I   ES       R++   +V 
Sbjct: 459 ASGFHPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQES----PMRRIYRPEDVK 518

Query: 64  SILKKNSETDAVKAIKLDLSNDLSPTSID----AQAFRNIKNLKLLILRNITFSSD-MFE 123
                +   +A++ + L          ++    A+A +  + L++L+        D    
Sbjct: 519 DACIGDMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVA 578

Query: 124 YSLNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSY 183
           Y  N   W+  R     +S P+NF     LV L M+ S I  L N  +    L  +DLSY
Sbjct: 579 YLPNSLLWLEWR-NYSSNSFPSNF-EPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSY 638

Query: 184 CELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNLEKLPSYIRL 243
           C  L   PD     NLE L L  C +L  +H SV  L  L+ L+++ C++LE+LP+ I+ 
Sbjct: 639 CHKLIQTPDFRMITNLERLILSSCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQS 698

Query: 244 KYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLIGCAN 303
           + L+   L+ C  L+  PE + NM  L ++ L  T I ELP+SIEHL+ LE L +  C  
Sbjct: 699 ECLEVLDLNYCFNLKMFPEVERNMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQ 758

Query: 304 LLALPSEFF 308
           L++LPS  +
Sbjct: 759 LVSLPSSIW 761

BLAST of Chy10G178550 vs. ExPASy Swiss-Prot
Match: F4JT78 (Disease resistance protein RPP2A OS=Arabidopsis thaliana OX=3702 GN=RPP2A PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.0e-33
Identity = 108/314 (34.39%), Postives = 162/314 (51.59%), Query Frame = 0

Query: 1    MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
            +L AC      GI  L D SL+S+ + K++M    Q MG  IV  E  D   RSRLW + 
Sbjct: 997  LLDACGFFTYMGICELIDESLISLVDNKIEMPIPFQDMGRIIVHEEDEDPCERSRLWDSK 1056

Query: 61   EVISILKKNSETDAVKAIKLDLSN---DLSPTSIDAQAFRNIKNLKLLILRNITFSSD-- 120
            +++ +L  NS T+A++ I LD S+   +LSPT      F  + NL+LL     T  +   
Sbjct: 1057 DIVDVLTNNSGTEAIEGIFLDASDLTCELSPT-----VFGKMYNLRLLKFYCSTSGNQCK 1116

Query: 121  -MFEYSLNKFKWVLSRFGIKFSSLPANFLVKD----GLVGLDMQHSCIKHLGNRFENCEG 180
                + L+     LS   + + + P  +L +      LV L+M +S ++ L    +N E 
Sbjct: 1117 LTLPHGLDTLPDELSL--LHWENYPLVYLPQKFNPVNLVELNMPYSNMEKLWEGKKNLEK 1176

Query: 181  LKYVDLSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNLE 240
            LK + LS+   L DI  LS A+NLE++ L GC SL  +  S+    KLV+L+++ C  L 
Sbjct: 1177 LKNIKLSHSRELTDILMLSEALNLEHIDLEGCTSLIDVSMSIPCCGKLVSLNMKDCSRLR 1236

Query: 241  KLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEF 300
             LPS + L  L    LSGC + + + +F  N++ +    L  T+I ELP SI +L +L  
Sbjct: 1237 SLPSMVDLTTLKLLNLSGCSEFEDIQDFAPNLEEI---YLAGTSIRELPLSIRNLTELVT 1296

Query: 301  LSLIGCANLLALPS 305
            L L  C  L  +PS
Sbjct: 1297 LDLENCERLQEMPS 1300

BLAST of Chy10G178550 vs. ExPASy TrEMBL
Match: A0A0A0KM44 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G207920 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.9e-115
Identity = 212/240 (88.33%), Postives = 226/240 (94.17%), Query Frame = 0

Query: 72  TDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYSLNKFKWVLSRF 131
           TDAVK IKLDLSNDLSPTSIDAQAF+N+KNLKLLILRNITFSS MFEY LNKFKWVLS F
Sbjct: 4   TDAVKVIKLDLSNDLSPTSIDAQAFKNMKNLKLLILRNITFSSHMFEYGLNKFKWVLSSF 63

Query: 132 GIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSYCELLEDIPDLSAA 191
           GI+FSSLPA+FLVK+GLVGLDMQH CIK+LGNRFENCE LK+VDLSYCELLE+IPDLS A
Sbjct: 64  GIRFSSLPASFLVKEGLVGLDMQHCCIKYLGNRFENCERLKHVDLSYCELLEEIPDLSVA 123

Query: 192 VNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNLEKLPSYIRLKYLDSFCLSGCRK 251
           VNLEYLYLRGC+SLK IHESV+SLNKL+TLDLEGCVNLEKLPSY+ LK LDSFCLS CRK
Sbjct: 124 VNLEYLYLRGCISLKTIHESVSSLNKLITLDLEGCVNLEKLPSYLMLKSLDSFCLSDCRK 183

Query: 252 LQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLIGCANLLALPSEFFLLES 311
           LQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSL GCANL+ALPSE +LL S
Sbjct: 184 LQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLKGCANLVALPSEIYLLRS 243

BLAST of Chy10G178550 vs. ExPASy TrEMBL
Match: A0A1S3C088 (TMV resistance protein N-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495388 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 2.8e-74
Identity = 172/382 (45.03%), Postives = 220/382 (57.59%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC LN D+GI  L DLSLV+IE  +VQMH+LIQQMG +IV  ES + G+RSRLW+  
Sbjct: 497 MLSACHLNIDFGIMILVDLSLVTIETDRVQMHELIQQMGRSIVHNESSEPGKRSRLWLVQ 556

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           ++  +   NS TDAVKAIKLDL N  +  ++D QAFR++KNL+LLI+RN  F   + +Y 
Sbjct: 557 DIWEVFVNNSGTDAVKAIKLDLPNP-TKLNVDPQAFRSMKNLRLLIIRNAQFCRKI-KYL 616

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLS--- 180
            N  KW+  R G    SLP+ F+ K+ LVGLD++HS IK  G R E CE LK+VDLS   
Sbjct: 617 PNSLKWIEWR-GFAHRSLPSCFITKN-LVGLDLRHSSIKRFGKRLEGCERLKHVDLSYST 676

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 677 LLEQINDFSPASNLEELHLINCTNLGMIDKSVFSLYKLSVLNLDGCCNLQKLPRGYFMLS 736

Query: 241 --------YCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                   YC+ LE IPDLSAA NL+ LYL+ C +L++IHESV SL+KL  LDL  C  L
Sbjct: 737 SLKELNLCYCKKLEKIPDLSAASNLKRLYLQECTNLRVIHESVGSLDKLNHLDLRQCTKL 796

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 312
            KLPSY+RLK L +  LSGC KL+  P   ENMKSL  + +D+TAI+ELPSSI +L  L 
Sbjct: 797 VKLPSYLRLKSLSNLLLSGCCKLESFPTIAENMKSLRELDMDFTAIKELPSSIGYLTNLS 856

BLAST of Chy10G178550 vs. ExPASy TrEMBL
Match: A0A1S3C093 (TMV resistance protein N-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495388 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 2.8e-74
Identity = 172/382 (45.03%), Postives = 220/382 (57.59%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC LN D+GI  L DLSLV+IE  +VQMH+LIQQMG +IV  ES + G+RSRLW+  
Sbjct: 310 MLSACHLNIDFGIMILVDLSLVTIETDRVQMHELIQQMGRSIVHNESSEPGKRSRLWLVQ 369

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           ++  +   NS TDAVKAIKLDL N  +  ++D QAFR++KNL+LLI+RN  F   + +Y 
Sbjct: 370 DIWEVFVNNSGTDAVKAIKLDLPNP-TKLNVDPQAFRSMKNLRLLIIRNAQFCRKI-KYL 429

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLS--- 180
            N  KW+  R G    SLP+ F+ K+ LVGLD++HS IK  G R E CE LK+VDLS   
Sbjct: 430 PNSLKWIEWR-GFAHRSLPSCFITKN-LVGLDLRHSSIKRFGKRLEGCERLKHVDLSYST 489

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 490 LLEQINDFSPASNLEELHLINCTNLGMIDKSVFSLYKLSVLNLDGCCNLQKLPRGYFMLS 549

Query: 241 --------YCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                   YC+ LE IPDLSAA NL+ LYL+ C +L++IHESV SL+KL  LDL  C  L
Sbjct: 550 SLKELNLCYCKKLEKIPDLSAASNLKRLYLQECTNLRVIHESVGSLDKLNHLDLRQCTKL 609

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 312
            KLPSY+RLK L +  LSGC KL+  P   ENMKSL  + +D+TAI+ELPSSI +L  L 
Sbjct: 610 VKLPSYLRLKSLSNLLLSGCCKLESFPTIAENMKSLRELDMDFTAIKELPSSIGYLTNLS 669

BLAST of Chy10G178550 vs. ExPASy TrEMBL
Match: A0A5A7TWU2 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00320 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 6.2e-74
Identity = 173/382 (45.29%), Postives = 223/382 (58.38%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC +N D+GI  L DLSL++IEN KVQMHDLI+QMGH IV GES +LG+RSRLW+  
Sbjct: 474 MLSACHVNLDFGIIVLMDLSLMTIENDKVQMHDLIKQMGHKIVCGESLELGKRSRLWLVQ 533

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           +V  +L  NS TDAVKAIK D  N  +   +D QAFR +KNL+LLI++N  F + + EY 
Sbjct: 534 DVWDVLVNNSGTDAVKAIKFDFPNP-TKLDVDLQAFRKMKNLRLLIVQNARFCTKI-EYL 593

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVD----- 180
            +  KW+    G   S+LP+ F+ K+ LVGLD+QHS IK    R ++CE LK+VD     
Sbjct: 594 PDSLKWI-KWHGFPQSTLPSCFITKN-LVGLDLQHSFIKTFEKRLKDCERLKHVDLSYST 653

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 654 LLEQIPDFSAASNLGELYLINCTNLGMIDKSLFSLNKLIVLNLDGCSNLKKFPRGYFMLS 713

Query: 241 ------LSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                 LSYC+ LE IPDLSAA NLE LYL+ C +L++IHESV SL+KL  LDL  C NL
Sbjct: 714 SLKELRLSYCKKLEKIPDLSAASNLERLYLQECTNLRLIHESVGSLDKLDHLDLRQCTNL 773

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 312
            KLPS++RLK L +  LS C KL+  P  DENMKSL  + LD+TAI+ELPSSI +L +L 
Sbjct: 774 SKLPSHLRLKSLQNLELSRCCKLESFPTIDENMKSLRHLDLDFTAIKELPSSIGYLTELC 833

BLAST of Chy10G178550 vs. ExPASy TrEMBL
Match: A0A5D3D437 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00140 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 8.1e-74
Identity = 173/382 (45.29%), Postives = 224/382 (58.64%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC +N D+GI  L DLSL++IEN KVQMHDLI+QMGH IV GES +LG+RSRLW+  
Sbjct: 474 MLSACHVNLDFGIIVLMDLSLMTIENDKVQMHDLIKQMGHKIVCGESLELGKRSRLWLVQ 533

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           +V  +L  NS TDAVKAIKLD  N  +   +D QAFR +KNL+LLI++N  F + + EY 
Sbjct: 534 DVWDVLVNNSGTDAVKAIKLDFPNP-TKLDVDLQAFRKMKNLRLLIVQNARFCTKI-EYL 593

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVD----- 180
            +  KW+    G   S+LP+ F+ K+ LVGLD+QHS IK    R ++CE LK+VD     
Sbjct: 594 PDSLKWI-KWHGFPQSTLPSCFITKN-LVGLDLQHSFIKTFEKRLKDCERLKHVDLSYST 653

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 654 LLEQIPDFSAASNLGELYLINCTNLGMIDKSLFSLNNLIVLNLDGCSNLKKFPRGYFMLS 713

Query: 241 ------LSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                 LSYC+ LE IP+LSAA NLE LYL+ C +L++IHESV SL+KL  LDL  C NL
Sbjct: 714 SLKELRLSYCKKLEKIPNLSAASNLERLYLQECTNLRLIHESVGSLDKLDHLDLRQCTNL 773

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 312
            KLPS++RLK L +  LS C KL+  P  DENMKSL  + LD+TAI+ELPSSI +L +L 
Sbjct: 774 SKLPSHLRLKSLQNLELSRCCKLESFPTIDENMKSLRHLDLDFTAIKELPSSIGYLTELC 833

BLAST of Chy10G178550 vs. NCBI nr
Match: XP_031741270.1 (TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 505 bits (1301), Expect = 1.60e-172
Identity = 260/311 (83.60%), Postives = 275/311 (88.42%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML A  LNPDYGIT L DLSL+SIENGKVQMHDLI QMGHTIVRGESFDL  RSRLW   
Sbjct: 97  MLKARDLNPDYGITVLNDLSLISIENGKVQMHDLIPQMGHTIVRGESFDLENRSRLW--- 156

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
                      TDAVK IKLDLSNDLSPTSIDAQAF+N+KNLKLLILRNITFSS MFEY 
Sbjct: 157 ----------GTDAVKVIKLDLSNDLSPTSIDAQAFKNMKNLKLLILRNITFSSHMFEYG 216

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSYCE 180
           LNKFKWVLS FGI+FSSLPA+FLVK+GLVGLDMQH CIK+LGNRFENCE LK+VDLSYCE
Sbjct: 217 LNKFKWVLSSFGIRFSSLPASFLVKEGLVGLDMQHCCIKYLGNRFENCERLKHVDLSYCE 276

Query: 181 LLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNLEKLPSYIRLKY 240
           LLE+IPDLS AVNLEYLYLRGC+SLK IHESV+SLNKL+TLDLEGCVNLEKLPSY+ LK 
Sbjct: 277 LLEEIPDLSVAVNLEYLYLRGCISLKTIHESVSSLNKLITLDLEGCVNLEKLPSYLMLKS 336

Query: 241 LDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLIGCANLL 300
           LDSFCLS CRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSL GCANL+
Sbjct: 337 LDSFCLSDCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLKGCANLV 394

Query: 301 ALPSEFFLLES 311
           ALPSE +LL S
Sbjct: 397 ALPSEIYLLRS 394

BLAST of Chy10G178550 vs. NCBI nr
Match: XP_008455159.1 (PREDICTED: TMV resistance protein N-like isoform X2 [Cucumis melo])

HSP 1 Score: 285 bits (730), Expect = 1.28e-83
Identity = 172/382 (45.03%), Postives = 220/382 (57.59%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC LN D+GI  L DLSLV+IE  +VQMH+LIQQMG +IV  ES + G+RSRLW+  
Sbjct: 310 MLSACHLNIDFGIMILVDLSLVTIETDRVQMHELIQQMGRSIVHNESSEPGKRSRLWLVQ 369

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           ++  +   NS TDAVKAIKLDL N  +  ++D QAFR++KNL+LLI+RN  F   + +Y 
Sbjct: 370 DIWEVFVNNSGTDAVKAIKLDLPNP-TKLNVDPQAFRSMKNLRLLIIRNAQFCRKI-KYL 429

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSY-- 180
            N  KW+  R G    SLP+ F+ K+ LVGLD++HS IK  G R E CE LK+VDLSY  
Sbjct: 430 PNSLKWIEWR-GFAHRSLPSCFITKN-LVGLDLRHSSIKRFGKRLEGCERLKHVDLSYST 489

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 490 LLEQINDFSPASNLEELHLINCTNLGMIDKSVFSLYKLSVLNLDGCCNLQKLPRGYFMLS 549

Query: 241 ---------CELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                    C+ LE IPDLSAA NL+ LYL+ C +L++IHESV SL+KL  LDL  C  L
Sbjct: 550 SLKELNLCYCKKLEKIPDLSAASNLKRLYLQECTNLRVIHESVGSLDKLNHLDLRQCTKL 609

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 311
            KLPSY+RLK L +  LSGC KL+  P   ENMKSL  + +D+TAI+ELPSSI +L  L 
Sbjct: 610 VKLPSYLRLKSLSNLLLSGCCKLESFPTIAENMKSLRELDMDFTAIKELPSSIGYLTNLS 669

BLAST of Chy10G178550 vs. NCBI nr
Match: XP_008455151.1 (PREDICTED: TMV resistance protein N-like isoform X1 [Cucumis melo])

HSP 1 Score: 285 bits (730), Expect = 3.87e-83
Identity = 172/382 (45.03%), Postives = 220/382 (57.59%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC LN D+GI  L DLSLV+IE  +VQMH+LIQQMG +IV  ES + G+RSRLW+  
Sbjct: 497 MLSACHLNIDFGIMILVDLSLVTIETDRVQMHELIQQMGRSIVHNESSEPGKRSRLWLVQ 556

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           ++  +   NS TDAVKAIKLDL N  +  ++D QAFR++KNL+LLI+RN  F   + +Y 
Sbjct: 557 DIWEVFVNNSGTDAVKAIKLDLPNP-TKLNVDPQAFRSMKNLRLLIIRNAQFCRKI-KYL 616

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSY-- 180
            N  KW+  R G    SLP+ F+ K+ LVGLD++HS IK  G R E CE LK+VDLSY  
Sbjct: 617 PNSLKWIEWR-GFAHRSLPSCFITKN-LVGLDLRHSSIKRFGKRLEGCERLKHVDLSYST 676

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 677 LLEQINDFSPASNLEELHLINCTNLGMIDKSVFSLYKLSVLNLDGCCNLQKLPRGYFMLS 736

Query: 241 ---------CELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                    C+ LE IPDLSAA NL+ LYL+ C +L++IHESV SL+KL  LDL  C  L
Sbjct: 737 SLKELNLCYCKKLEKIPDLSAASNLKRLYLQECTNLRVIHESVGSLDKLNHLDLRQCTKL 796

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 311
            KLPSY+RLK L +  LSGC KL+  P   ENMKSL  + +D+TAI+ELPSSI +L  L 
Sbjct: 797 VKLPSYLRLKSLSNLLLSGCCKLESFPTIAENMKSLRELDMDFTAIKELPSSIGYLTNLS 856

BLAST of Chy10G178550 vs. NCBI nr
Match: KAA0047972.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 284 bits (727), Expect = 9.29e-83
Identity = 173/382 (45.29%), Postives = 223/382 (58.38%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC +N D+GI  L DLSL++IEN KVQMHDLI+QMGH IV GES +LG+RSRLW+  
Sbjct: 474 MLSACHVNLDFGIIVLMDLSLMTIENDKVQMHDLIKQMGHKIVCGESLELGKRSRLWLVQ 533

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           +V  +L  NS TDAVKAIK D  N  +   +D QAFR +KNL+LLI++N  F + + EY 
Sbjct: 534 DVWDVLVNNSGTDAVKAIKFDFPNP-TKLDVDLQAFRKMKNLRLLIVQNARFCTKI-EYL 593

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSY-- 180
            +  KW+    G   S+LP+ F+ K+ LVGLD+QHS IK    R ++CE LK+VDLSY  
Sbjct: 594 PDSLKWIKWH-GFPQSTLPSCFITKN-LVGLDLQHSFIKTFEKRLKDCERLKHVDLSYST 653

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 654 LLEQIPDFSAASNLGELYLINCTNLGMIDKSLFSLNKLIVLNLDGCSNLKKFPRGYFMLS 713

Query: 241 ---------CELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                    C+ LE IPDLSAA NLE LYL+ C +L++IHESV SL+KL  LDL  C NL
Sbjct: 714 SLKELRLSYCKKLEKIPDLSAASNLERLYLQECTNLRLIHESVGSLDKLDHLDLRQCTNL 773

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 311
            KLPS++RLK L +  LS C KL+  P  DENMKSL  + LD+TAI+ELPSSI +L +L 
Sbjct: 774 SKLPSHLRLKSLQNLELSRCCKLESFPTIDENMKSLRHLDLDFTAIKELPSSIGYLTELC 833

BLAST of Chy10G178550 vs. NCBI nr
Match: XP_016901470.1 (PREDICTED: LOW QUALITY PROTEIN: disease resistance protein RPP4-like [Cucumis melo])

HSP 1 Score: 283 bits (725), Expect = 9.81e-83
Identity = 173/382 (45.29%), Postives = 224/382 (58.64%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESFDLGRRSRLWVAN 60
           ML+AC +N D+GI  L DLSL++IEN KVQM+DLI+QMGH IV GES +LG+RSRLW+  
Sbjct: 495 MLSACHVNLDFGIIVLMDLSLMTIENDKVQMYDLIKQMGHKIVCGESLELGKRSRLWLVR 554

Query: 61  EVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEYS 120
           +V  +L  NS TDAVKAIKLD  N  +   +D QAFR +KNL+LLI++N  F + + EY 
Sbjct: 555 DVWEVLVNNSGTDAVKAIKLDFPNP-TKLDVDLQAFRKMKNLRLLIVQNARFCTKI-EYL 614

Query: 121 LNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKYVDLSY-- 180
            +  KW+    G   S+LP+ F+ K+ LVGLD+QHS IK    R ++CE LK+VDLSY  
Sbjct: 615 PDSLKWIKWH-GFPQSTLPSCFITKN-LVGLDLQHSFIKTFEKRLKDCERLKHVDLSYST 674

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 675 LLEQIPDFSAASNLGELYLINCTNLGMIDKSLFSLNNLIVLNLDGCSNLKKFPRGYFMLS 734

Query: 241 ---------CELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNL 300
                    C+ LE IPDLSAA NLE LYL+ C +L++IHESV SL+KL  LDL  C NL
Sbjct: 735 SLKELRLSYCKKLEKIPDLSAASNLERLYLQECTNLRLIHESVGSLDKLDHLDLRQCTNL 794

Query: 301 EKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLE 311
            KLPS++RLK L +  LS C KL+  P  DENMKSL  + LD+TAI+ELPSSI +L +L 
Sbjct: 795 SKLPSHLRLKSLQNLELSRCCKLESFPTIDENMKSLRHLDLDFTAIKELPSSIGYLTELC 854

BLAST of Chy10G178550 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 176.8 bits (447), Expect = 3.0e-44
Identity = 112/307 (36.48%), Postives = 174/307 (56.68%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGES-FDLGRRSRLWVA 60
           +L  C L PD  ++ L +  L++I    + MHDL++ MG  IVR  S    G RSRLW  
Sbjct: 452 ILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSH 511

Query: 61  NEVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEY 120
           N+V+ +LKK S T+A++ + L  ++ +     + +AF  ++ L+LL LR +  +   +E+
Sbjct: 512 NDVVGVLKKKSGTNAIEGLSLK-ADVMDFQYFEVEAFAKMQELRLLELRYVDLNGS-YEH 571

Query: 121 SLNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRF---ENCEGLKYVDL 180
                +W L   G      P N L  + L  LD+Q+S +K         +    +KY+DL
Sbjct: 572 FPKDLRW-LCWHGFSLECFPIN-LSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDL 631

Query: 181 SYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLN-KLVTLDLEGCVNLEKLPSY 240
           S+   L + PD S   N+E L L  C SL ++H+S+  L+ KLV L+L  C+ L+ LP  
Sbjct: 632 SHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEE 691

Query: 241 I-RLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLI 300
           I +LK L+S  LS C KL+R+ +    ++SLT ++ D+TA+ E+PS+I  L KL+ LSL 
Sbjct: 692 IYKLKSLESLFLSNCSKLERLDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLN 751

Query: 301 GCANLLA 302
           GC  LL+
Sbjct: 752 GCKGLLS 754

BLAST of Chy10G178550 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 176.8 bits (447), Expect = 3.0e-44
Identity = 112/307 (36.48%), Postives = 174/307 (56.68%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGES-FDLGRRSRLWVA 60
           +L  C L PD  ++ L +  L++I    + MHDL++ MG  IVR  S    G RSRLW  
Sbjct: 455 ILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSH 514

Query: 61  NEVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEY 120
           N+V+ +LKK S T+A++ + L  ++ +     + +AF  ++ L+LL LR +  +   +E+
Sbjct: 515 NDVVGVLKKKSGTNAIEGLSLK-ADVMDFQYFEVEAFAKMQELRLLELRYVDLNGS-YEH 574

Query: 121 SLNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRF---ENCEGLKYVDL 180
                +W L   G      P N L  + L  LD+Q+S +K         +    +KY+DL
Sbjct: 575 FPKDLRW-LCWHGFSLECFPIN-LSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDL 634

Query: 181 SYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLN-KLVTLDLEGCVNLEKLPSY 240
           S+   L + PD S   N+E L L  C SL ++H+S+  L+ KLV L+L  C+ L+ LP  
Sbjct: 635 SHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEE 694

Query: 241 I-RLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSLI 300
           I +LK L+S  LS C KL+R+ +    ++SLT ++ D+TA+ E+PS+I  L KL+ LSL 
Sbjct: 695 IYKLKSLESLFLSNCSKLERLDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLN 754

Query: 301 GCANLLA 302
           GC  LL+
Sbjct: 755 GCKGLLS 757

BLAST of Chy10G178550 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-40
Identity = 113/338 (33.43%), Postives = 176/338 (52.07%), Query Frame = 0

Query: 1   MLTACALNPDYGITALTDLSLVSIENGKVQMHDLIQQMGHTIVRGESF-DLGRRSRLWVA 60
           +L  C    + GIT LT+ SL+   NG V++HDL++QMG  +VR ++  +  +R  LW  
Sbjct: 452 LLDLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDP 511

Query: 61  NEVISILKKNSETDAVKAIKLDLSNDLSPTSIDAQAFRNIKNLKLLILRNITFSSDMFEY 120
            ++  +L +NS T  V+ I L+LS ++S      +AF  + NLKLL   +++F  +   +
Sbjct: 512 EDICHLLSENSGTQLVEGISLNLS-EISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVH 571

Query: 121 SLNKFKWVLSRF------GIKFSSLPANFLVKDGLVGLDMQHSCIKHLGNRFENCEGLKY 180
             N   ++  +       G    ++P+ F   + LV L M +S ++ L +  +    LK 
Sbjct: 572 LPNGLSYLPRKLRYLRWDGYPLKTMPSRFF-PEFLVELCMSNSNLEKLWDGIQPLRNLKK 631

Query: 181 VDLSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLVTLDLEGCVNLEKLP 240
           +DLS C+ L ++PDLS A NLE L L  C SL  +  S+ +L  L    L  C+ L+ +P
Sbjct: 632 MDLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIP 691

Query: 241 SYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELPSSIEHLAKLEFLSL 300
             I LK L++  +SGC  L+  PE   N +   R+ L  T IEELPSSI  L+ L  L +
Sbjct: 692 IGIILKSLETVGMSGCSSLKHFPEISWNTR---RLYLSSTKIEELPSSISRLSCLVKLDM 751

Query: 301 IGCANLLALPSEFFLLESCATRHSLSNNYPTNIPSSLK 332
             C  L  LPS    L S  + +        N+P +L+
Sbjct: 752 SDCQRLRTLPSYLGHLVSLKSLNLDGCRRLENLPDTLQ 784

BLAST of Chy10G178550 vs. TAIR 10
Match: AT4G12020.1 (protein kinase family protein )

HSP 1 Score: 155.6 bits (392), Expect = 7.0e-38
Identity = 121/335 (36.12%), Postives = 175/335 (52.24%), Query Frame = 0

Query: 1    MLTACALNPDYGITALTDLSLVSI-ENGKVQMHDLIQQMGHTIVRGESFDL-GRRSRLWV 60
            +L  C  +   G   L D SL++I ++  V M   IQ  G  IVR ES D  G RSRLW 
Sbjct: 1077 LLDGCGFSAHVGFRGLVDKSLLTISQHNLVDMLSFIQATGREIVRQESADRPGDRSRLWN 1136

Query: 61   ANEVISILKKNSETDAVKAIKLDLSN---DLSPTSIDAQAFRNIKNLKLLIL-------- 120
            A+ +  +   ++ T A++ I LD+ N   D +P       F  + NL+LL L        
Sbjct: 1137 ADYIRHVFINDTGTSAIEGIFLDMLNLKFDANP-----NVFEKMCNLRLLKLYCSKAEEK 1196

Query: 121  RNITFSSDMFEYSLNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHL--GNRF 180
              ++F   + EY  +K + +   +    SSLP +F   + LV L++  SC K L  G + 
Sbjct: 1197 HGVSFPQGL-EYLPSKLRLLHWEY-YPLSSLPKSF-NPENLVELNLPSSCAKKLWKGKKA 1256

Query: 181  ENC------EGLKYVDLSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLV 240
              C      E LK + LSY + L  IP LS+A NLE++ L GC SL  + +S++ L KLV
Sbjct: 1257 RFCTTNSSLEKLKKMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSISYLKKLV 1316

Query: 241  TLDLEGCVNLEKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELP 300
             L+L+GC  LE +PS + L+ L+   LSGC KL   PE   N+K L    +  T I+E+P
Sbjct: 1317 FLNLKGCSKLENIPSMVDLESLEVLNLSGCSKLGNFPEISPNVKEL---YMGGTMIQEIP 1376

Query: 301  SSIEHLAKLEFLSLIGCANLLALPSEFFLLESCAT 315
            SSI++L  LE L L    +L  LP+  + L+   T
Sbjct: 1377 SSIKNLVLLEKLDLENSRHLKNLPTSIYKLKHLET 1400

BLAST of Chy10G178550 vs. TAIR 10
Match: AT4G12020.2 (protein kinase family protein )

HSP 1 Score: 155.6 bits (392), Expect = 7.0e-38
Identity = 121/335 (36.12%), Postives = 175/335 (52.24%), Query Frame = 0

Query: 1    MLTACALNPDYGITALTDLSLVSI-ENGKVQMHDLIQQMGHTIVRGESFDL-GRRSRLWV 60
            +L  C  +   G   L D SL++I ++  V M   IQ  G  IVR ES D  G RSRLW 
Sbjct: 1077 LLDGCGFSAHVGFRGLVDKSLLTISQHNLVDMLSFIQATGREIVRQESADRPGDRSRLWN 1136

Query: 61   ANEVISILKKNSETDAVKAIKLDLSN---DLSPTSIDAQAFRNIKNLKLLIL-------- 120
            A+ +  +   ++ T A++ I LD+ N   D +P       F  + NL+LL L        
Sbjct: 1137 ADYIRHVFINDTGTSAIEGIFLDMLNLKFDANP-----NVFEKMCNLRLLKLYCSKAEEK 1196

Query: 121  RNITFSSDMFEYSLNKFKWVLSRFGIKFSSLPANFLVKDGLVGLDMQHSCIKHL--GNRF 180
              ++F   + EY  +K + +   +    SSLP +F   + LV L++  SC K L  G + 
Sbjct: 1197 HGVSFPQGL-EYLPSKLRLLHWEY-YPLSSLPKSF-NPENLVELNLPSSCAKKLWKGKKA 1256

Query: 181  ENC------EGLKYVDLSYCELLEDIPDLSAAVNLEYLYLRGCVSLKMIHESVASLNKLV 240
              C      E LK + LSY + L  IP LS+A NLE++ L GC SL  + +S++ L KLV
Sbjct: 1257 RFCTTNSSLEKLKKMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSISYLKKLV 1316

Query: 241  TLDLEGCVNLEKLPSYIRLKYLDSFCLSGCRKLQRVPEFDENMKSLTRMILDYTAIEELP 300
             L+L+GC  LE +PS + L+ L+   LSGC KL   PE   N+K L    +  T I+E+P
Sbjct: 1317 FLNLKGCSKLENIPSMVDLESLEVLNLSGCSKLGNFPEISPNVKEL---YMGGTMIQEIP 1376

Query: 301  SSIEHLAKLEFLSLIGCANLLALPSEFFLLESCAT 315
            SSI++L  LE L L    +L  LP+  + L+   T
Sbjct: 1377 SSIKNLVLLEKLDLENSRHLKNLPTSIYKLKHLET 1400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
V9M3981.5e-3734.90Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... [more]
Q9SZ679.9e-3736.12Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 ... [more]
V9M2S52.2e-3634.51Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
A0A290U7C47.9e-3432.36Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... [more]
F4JT781.0e-3334.39Disease resistance protein RPP2A OS=Arabidopsis thaliana OX=3702 GN=RPP2A PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0A0KM441.9e-11588.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G207920 PE=4 SV=1[more]
A0A1S3C0882.8e-7445.03TMV resistance protein N-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495388... [more]
A0A1S3C0932.8e-7445.03TMV resistance protein N-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495388... [more]
A0A5A7TWU26.2e-7445.29TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A5D3D4378.1e-7445.29TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
XP_031741270.11.60e-17283.60TMV resistance protein N-like [Cucumis sativus][more]
XP_008455159.11.28e-8345.03PREDICTED: TMV resistance protein N-like isoform X2 [Cucumis melo][more]
XP_008455151.13.87e-8345.03PREDICTED: TMV resistance protein N-like isoform X1 [Cucumis melo][more]
KAA0047972.19.29e-8345.29TMV resistance protein N-like [Cucumis melo var. makuwa][more]
XP_016901470.19.81e-8345.29PREDICTED: LOW QUALITY PROTEIN: disease resistance protein RPP4-like [Cucumis me... [more]
Match NameE-valueIdentityDescription
AT5G36930.13.0e-4436.48Disease resistance protein (TIR-NBS-LRR class) family [more]
AT5G36930.23.0e-4436.48Disease resistance protein (TIR-NBS-LRR class) family [more]
AT5G17680.11.5e-4033.43disease resistance protein (TIR-NBS-LRR class), putative [more]
AT4G12020.17.0e-3836.12protein kinase family protein [more]
AT4G12020.27.0e-3836.12protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 240..322
e-value: 3.9E-11
score: 44.0
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 51..239
e-value: 5.5E-18
score: 66.6
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 1..312
NoneNo IPR availablePANTHERPTHR11017:SF243RESISTANCE PROTEIN (TIR-NBS-LRR CLASS), PUTATIVE-RELATEDcoord: 1..312
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 30..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G178550.1Chy10G178550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0016071 mRNA metabolic process
biological_process GO:0009451 RNA modification