Chy10G178540 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G178540
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	GRAM domain-containing protein
Location	chrH10: 13088073 .. 13089026 (+)
RNA-Seq Expression	Chy10G178540
Synteny	Chy10G178540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAACTACAATTCAAGAACATATGATGGGATTTCCAATGGCATTGGCTTTGTCACGAGCTAGATATTTGAGTCATCCTTTGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATGTAAGAGTGCATAACAAAAAAATATTTTATTTTGGTTGAATAGATGTAAAATAGTTTTCTAACTAAATTTGGTTTTTGAATTGCTTGTAGATAATTGAACAGATTGCATCTTGAATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCACTCCGAGAACATGGTAAGTCTTTTTTTATAGATATCATCGATCTGGTGCATTATGCAAAAGTGTAATTAATTTTGATGATTTGAGGTTGAATTTTGCAGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTTCTTTTTGTAGTGATAAATCGATCAAAATCGCTTCCCCTAATGGAGATCATATCAGAATTCACTATAAAGTAAGTCTCTAATTTATATATATACACACTAAAATACCACTGTTTTGAATTAATTTGGTCAATTAAAATTTTCATTAGTACTGATGAAAGTTAATTAATGTTTGCATTACAGGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGTGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTCAATTATCAATCAACTTTCAACTCTCTTCAGGAAGTAGCTAGATGA

mRNA sequence

ATGAAAACTACAATTCAAGAACATATGATGGGATTTCCAATGGCATTGGCTTTGTCACGAGCTAGATATTTGAGTCATCCTTTGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATATTGCATCTTGAATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCACTCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTTCTTTTTGTAGTGATAAATCGATCAAAATCGCTTCCCCTAATGGAGATCATATCAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGTGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTCAATTATCAATCAACTTTCAACTCTCTTCAGGAAGTAGCTAGATGA

Coding sequence (CDS)

ATGAAAACTACAATTCAAGAACATATGATGGGATTTCCAATGGCATTGGCTTTGTCACGAGCTAGATATTTGAGTCATCCTTTGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATATTGCATCTTGAATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCACTCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTTCTTTTTGTAGTGATAAATCGATCAAAATCGCTTCCCCTAATGGAGATCATATCAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGTGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTCAATTATCAATCAACTTTCAACTCTCTTCAGGAAGTAGCTAGATGA

Protein sequence

MKTTIQEHMMGFPMALALSRARYLSHPLNRLHLSSSDDGFQSSIKKYCILNRLNKNGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR*

Homology

[Close]

BLAST of Chy10G178540 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-51
Identity = 109/213 (51.17%), Postives = 154/213 (72.30%), Query Frame = 0


Query: 5   IQEHMMGFPMALALSRARYLSHP--LNRLHL-SSSDDGFQSSIKKYCILNRLNKNGKKTD 64
           +++ ++ +P A A +   YL  P   N+  + +SS    QS++K   IL R     KKTD
Sbjct: 6   VEQQVIRYPAAKA-TPVGYLPDPASFNKFRVPASSKKSEQSNVKS--ILKR-----KKTD 65

Query: 65  NIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYL 124
              + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF +SE EKL K+ QCYL
Sbjct: 66  GFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYL 125

Query: 125 STTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKK 184
           STTAGP+ GLLFIS+ K++FCS++SIK+ SP GD IR+HYKV IP  K+ RVN+S+N KK
Sbjct: 126 STTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKK 185

Query: 185 TSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
            S++Y+++ T+D F+FWFMGFL+YQ  FN L++
Sbjct: 186 PSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEK 210

[Close]

BLAST of Chy10G178540 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-51
Identity = 107/215 (49.77%), Postives = 151/215 (70.23%), Query Frame = 0


Query: 5   IQEHMMGFPMALALSRARYLSHP--LNRLHLSSSDDGFQSSIKKYCILNRLNKN---GKK 64
           + + ++ FP A+  S A YL  P  +N+L + +S         K+  L    K+    KK
Sbjct: 6   VHQQLITFP-AVKTSPAGYLPDPASINKLQIPTS--------SKFSFLTGKGKSMLRKKK 65

Query: 65  TDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQC 124
            D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF +S+EEKL K  QC
Sbjct: 66  NDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQC 125

Query: 125 YLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENV 184
           YLSTTAGP+ GLLFIS+ KI+FCS++SIK+ASP G+  R+HYKV IP  K+  VN+S+N 
Sbjct: 126 YLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNT 185

Query: 185 KKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
            K S++Y+++ T+D F+FWFMGFL+YQ  FN L++
Sbjct: 186 TKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQ 211

[Close]

BLAST of Chy10G178540 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 8.6e-50
Identity = 93/157 (59.24%), Postives = 126/157 (80.25%), Query Frame = 0


Query: 58  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 117
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 55  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 114

Query: 118 QCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 177
           QCYLSTTAGP+ GLLFIS+ KI+FCS++SIK+ASP G   R+HYKV IP  K+  VN+S+
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 174

Query: 178 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
           N KK S++Y++I T+DNF+FWFMGF++YQ  FN L++
Sbjct: 175 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEK 211

[Close]

BLAST of Chy10G178540 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 5.6e-49
Identity = 101/212 (47.64%), Postives = 147/212 (69.34%), Query Frame = 0


Query: 5   IQEHMMGFPMALALSRARYLSHP--LNRLHLSSSDDGFQSSIKKYCILNRLNKNGKKTDN 64
           + + ++ FP A+  +   YL  P  +N+L + +S         K  +L +     KKTD+
Sbjct: 6   VHQQVIAFP-AVNTAPVGYLPDPASINKLQIPTS--------SKVSMLQK-----KKTDS 65

Query: 65  IIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLS 124
             +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  QCYLS
Sbjct: 66  FTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYLS 125

Query: 125 TTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKT 184
           TT G + GLLFIS+ KI+FCS++SIK+ SP GD  R+HYKV IP  K+  VN+S+N KK 
Sbjct: 126 TTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKKP 185

Query: 185 SERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
           S+RY+++ T+DN++FWFMGF++YQ  FN L++
Sbjct: 186 SQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEK 203

[Close]

BLAST of Chy10G178540 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 5.2e-31
Identity = 67/174 (38.51%), Postives = 111/174 (63.79%), Query Frame = 0


Query: 49  ILNRLNKNGKKTDN-------IIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIY 108
           +L  LN+ GKK ++       ++  L++H+K    IS+    +LS G +++  GG  +++
Sbjct: 49  VLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAMARLSQGTKMIVEGGPERVF 108

Query: 109 KKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKISFCSDKSIKI-ASPNGDHIRIH 168
           ++ F +   EKLL    CY+STT+GP+ G+++IS  +I+FCSD +I++ +S  G+ +  +
Sbjct: 109 QREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFCSDYAIRLPSSAGGNGVAAY 168

Query: 169 YKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
           YKVV+  EK+  ++ S NV K SERY+ + T D FEFWFMGF++Y   FN L +
Sbjct: 169 YKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAFNCLNK 222

[Close]

BLAST of Chy10G178540 vs. ExPASy TrEMBL
Match: A0A0A0KPM2 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579030 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.6e-102
Identity = 196/204 (96.08%), Postives = 198/204 (97.06%), Query Frame = 0


Query: 14  MALALSRARYLSHPLNRLHLSSSDDGFQSSIKKY----CILNRLNKNGKKTDNIIHALRE 73
           M+LALSRARYLSHP NRLHLSSSDDGFQSSIKKY    CIL RLNKNGKKTDNIIHALRE
Sbjct: 1   MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILKRLNKNGKKTDNIIHALRE 60

Query: 74  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 133
           HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP
Sbjct: 61  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 120

Query: 134 GLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 193
           GLLFISTHKI+FCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI
Sbjct: 121 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 180

Query: 194 ETLDNFEFWFMGFLNYQSTFNSLQ 214
           ETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 ETLDNFEFWFMGFLNYQSTFNSLQ 204

[Close]

BLAST of Chy10G178540 vs. ExPASy TrEMBL
Match: A0A1S3BEG1 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.6e-102
Identity = 195/217 (89.86%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MKTTIQEHMMGFPMALALSRARYLSHPLNRLHLSSSDDGFQSSIKKY----CILNRLNKN 60
           MKTTIQEHM+GF M+LALSRARYL HPL RLHLSSSDDG QSSIKKY    CILNRLN N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GK+TDNIIHALREHVKLG KISE VKGKLSLGA+ILRVGG+RKIYKKLFSM+EEEKLLKV
Sbjct: 61  GKRTDNIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPL GLLFISTHKI+FCSDKSIKIASPNGDHIRI YKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRILYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 214
           ENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 ENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQ 217

[Close]

BLAST of Chy10G178540 vs. ExPASy TrEMBL
Match: A0A5A7SSL2 (GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00700 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 199/222 (89.64%), Postives = 205/222 (92.34%), Query Frame = 0


Query: 3   TTIQEHMMGFPMALALSRARYLSH-PLNRLHLSSSDDGF-QSSIKKY----CILNRLNKN 62
           TTIQEHM+GF M+ ALSRARYL H P   LH+SSSDD   QSSIKKY    CILNRLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 122
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSM+EEEKLLKV
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASP-NGDHIRIHYKVVIPKEKVMRVNE 182
           SQCYLSTTAGPL GLLFISTHKI+FCSDKSIKIASP NGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNE 183

Query: 183 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 218
           SENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR
Sbjct: 184 SENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 225

[Close]

BLAST of Chy10G178540 vs. ExPASy TrEMBL
Match: A0A1S3CNX8 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 199/222 (89.64%), Postives = 205/222 (92.34%), Query Frame = 0


Query: 3   TTIQEHMMGFPMALALSRARYLSH-PLNRLHLSSSDDGF-QSSIKKY----CILNRLNKN 62
           TTIQEHM+GF M+ ALSRARYL H P   LH+SSSDD   QSSIKKY    CILNRLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 122
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSM+EEEKLLKV
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASP-NGDHIRIHYKVVIPKEKVMRVNE 182
           SQCYLSTTAGPL GLLFISTHKI+FCSDKSIKIASP NGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNE 183

Query: 183 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 218
           SENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR
Sbjct: 184 SENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 225

[Close]

BLAST of Chy10G178540 vs. ExPASy TrEMBL
Match: A0A0A0KT32 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579000 PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.1e-97
Identity = 192/209 (91.87%), Postives = 195/209 (93.30%), Query Frame = 0


Query: 14  MALALSRARYLSHPLNRLHLSSSDDGF-QSSIKKY----CILNRLNKNGKKTDNIIHALR 73
           M+LALSRAR L H    LH+SSSDD   QSSIKKY    CIL RLNKNGKKTDNIIHALR
Sbjct: 1   MSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNKNGKKTDNIIHALR 60

Query: 74  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL 133
           EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL
Sbjct: 61  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL 120

Query: 134 PGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ 193
           PGLLFISTHKI+FCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ
Sbjct: 121 PGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ 180

Query: 194 IETLDNFEFWFMGFLNYQSTFNSLQEVAR 218
           IETLDNFEFWFMGFLNYQSTFNSLQEVAR
Sbjct: 181 IETLDNFEFWFMGFLNYQSTFNSLQEVAR 209

[Close]

BLAST of Chy10G178540 vs. NCBI nr
Match: XP_011655497.2 (putative GEM-like protein 8 [Cucumis sativus])

HSP 1 Score: 407 bits (1045), Expect = 1.31e-142
Identity = 208/217 (95.85%), Postives = 212/217 (97.70%), Query Frame = 0


Query: 1   MKTTIQEHMMGFPMALALSRARYLSHPLNRLHLSSSDDGFQSSIKKY----CILNRLNKN 60
           MKTTIQEHM+GFPM+LALSRARYLSHP NRLHLSSSDDGFQSSIKKY    CIL+RLNKN
Sbjct: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV
Sbjct: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPLPGLLFISTHKI+FCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 213
           ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 217

[Close]

BLAST of Chy10G178540 vs. NCBI nr
Match: XP_011655499.1 (GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572 [Cucumis sativus])

HSP 1 Score: 388 bits (997), Expect = 3.13e-135
Identity = 203/222 (91.44%), Postives = 207/222 (93.24%), Query Frame = 0


Query: 1   MKTTIQEHMMGFPMALALSRARYLSHPLNRLHLSSSDDGF-QSSIKKY----CILNRLNK 60
           MKTTIQEHM+GF M+LALSRAR L H    LH+SSSDD   QSSIKKY    CIL RLNK
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120
           NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 VSQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE 180
           VSQCYLSTTAGPLPGLLFISTHKI+FCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE 180

Query: 181 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 217
           SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR
Sbjct: 181 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 222

[Close]

BLAST of Chy10G178540 vs. NCBI nr
Match: KGN52180.2 (hypothetical protein Csa_007871 [Cucumis sativus])

HSP 1 Score: 381 bits (978), Expect = 1.35e-132
Identity = 196/204 (96.08%), Postives = 199/204 (97.55%), Query Frame = 0


Query: 14  MALALSRARYLSHPLNRLHLSSSDDGFQSSIKKY----CILNRLNKNGKKTDNIIHALRE 73
           M+LALSRARYLSHP NRLHLSSSDDGFQSSIKKY    CIL+RLNKNGKKTDNIIHALRE
Sbjct: 1   MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALRE 60

Query: 74  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 133
           HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP
Sbjct: 61  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 120

Query: 134 GLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 193
           GLLFISTHKI+FCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI
Sbjct: 121 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 180

Query: 194 ETLDNFEFWFMGFLNYQSTFNSLQ 213
           ETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 ETLDNFEFWFMGFLNYQSTFNSLQ 204

[Close]

BLAST of Chy10G178540 vs. NCBI nr
Match: XP_008445894.1 (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 380 bits (976), Expect = 4.31e-132
Identity = 195/217 (89.86%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MKTTIQEHMMGFPMALALSRARYLSHPLNRLHLSSSDDGFQSSIKKY----CILNRLNKN 60
           MKTTIQEHM+GF M+LALSRARYL HPL RLHLSSSDDG QSSIKKY    CILNRLN N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GK+TDNIIHALREHVKLG KISE VKGKLSLGA+ILRVGG+RKIYKKLFSM+EEEKLLKV
Sbjct: 61  GKRTDNIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPL GLLFISTHKI+FCSDKSIKIASPNGDHIRI YKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRILYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 213
           ENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 ENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQ 217

[Close]

BLAST of Chy10G178540 vs. NCBI nr
Match: XP_008465496.1 (PREDICTED: GEM-like protein 4 [Cucumis melo] >KAA0034082.1 GEM-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 375 bits (964), Expect = 3.73e-130
Identity = 199/222 (89.64%), Postives = 205/222 (92.34%), Query Frame = 0


Query: 3   TTIQEHMMGFPMALALSRARYLSH-PLNRLHLSSSDDGF-QSSIKKY----CILNRLNKN 62
           TTIQEHM+GF M+ ALSRARYL H P   LH+SSSDD   QSSIKKY    CILNRLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 122
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSM+EEEKLLKV
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPN-GDHIRIHYKVVIPKEKVMRVNE 182
           SQCYLSTTAGPL GLLFISTHKI+FCSDKSIKIASPN GDHIRIHYKVVIPKEKVMRVNE
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNE 183

Query: 183 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 217
           SENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR
Sbjct: 184 SENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 225

[Close]

BLAST of Chy10G178540 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 204.5 bits (519), Expect = 8.5e-53
Identity = 109/213 (51.17%), Postives = 154/213 (72.30%), Query Frame = 0


Query: 5   IQEHMMGFPMALALSRARYLSHP--LNRLHL-SSSDDGFQSSIKKYCILNRLNKNGKKTD 64
           +++ ++ +P A A +   YL  P   N+  + +SS    QS++K   IL R     KKTD
Sbjct: 6   VEQQVIRYPAAKA-TPVGYLPDPASFNKFRVPASSKKSEQSNVKS--ILKR-----KKTD 65

Query: 65  NIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYL 124
              + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF +SE EKL K+ QCYL
Sbjct: 66  GFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYL 125

Query: 125 STTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKK 184
           STTAGP+ GLLFIS+ K++FCS++SIK+ SP GD IR+HYKV IP  K+ RVN+S+N KK
Sbjct: 126 STTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKK 185

Query: 185 TSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
            S++Y+++ T+D F+FWFMGFL+YQ  FN L++
Sbjct: 186 PSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEK 210

[Close]

BLAST of Chy10G178540 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 203.8 bits (517), Expect = 1.5e-52
Identity = 107/215 (49.77%), Postives = 151/215 (70.23%), Query Frame = 0


Query: 5   IQEHMMGFPMALALSRARYLSHP--LNRLHLSSSDDGFQSSIKKYCILNRLNKN---GKK 64
           + + ++ FP A+  S A YL  P  +N+L + +S         K+  L    K+    KK
Sbjct: 6   VHQQLITFP-AVKTSPAGYLPDPASINKLQIPTS--------SKFSFLTGKGKSMLRKKK 65

Query: 65  TDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQC 124
            D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF +S+EEKL K  QC
Sbjct: 66  NDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQC 125

Query: 125 YLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENV 184
           YLSTTAGP+ GLLFIS+ KI+FCS++SIK+ASP G+  R+HYKV IP  K+  VN+S+N 
Sbjct: 126 YLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNT 185

Query: 185 KKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
            K S++Y+++ T+D F+FWFMGFL+YQ  FN L++
Sbjct: 186 TKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQ 211

[Close]

BLAST of Chy10G178540 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 198.4 bits (503), Expect = 6.1e-51
Identity = 93/157 (59.24%), Postives = 126/157 (80.25%), Query Frame = 0


Query: 58  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 117
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 117 KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 176

Query: 118 QCYLSTTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 177
           QCYLSTTAGP+ GLLFIS+ KI+FCS++SIK+ASP G   R+HYKV IP  K+  VN+S+
Sbjct: 177 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 236

Query: 178 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
           N KK S++Y++I T+DNF+FWFMGF++YQ  FN L++
Sbjct: 237 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEK 273

[Close]

BLAST of Chy10G178540 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 195.7 bits (496), Expect = 4.0e-50
Identity = 101/212 (47.64%), Postives = 147/212 (69.34%), Query Frame = 0


Query: 5   IQEHMMGFPMALALSRARYLSHP--LNRLHLSSSDDGFQSSIKKYCILNRLNKNGKKTDN 64
           + + ++ FP A+  +   YL  P  +N+L + +S         K  +L +     KKTD+
Sbjct: 6   VHQQVIAFP-AVNTAPVGYLPDPASINKLQIPTS--------SKVSMLQK-----KKTDS 65

Query: 65  IIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLS 124
             +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  QCYLS
Sbjct: 66  FTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYLS 125

Query: 125 TTAGPLPGLLFISTHKISFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKT 184
           TT G + GLLFIS+ KI+FCS++SIK+ SP GD  R+HYKV IP  K+  VN+S+N KK 
Sbjct: 126 TTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKKP 185

Query: 185 SERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
           S+RY+++ T+DN++FWFMGF++YQ  FN L++
Sbjct: 186 SQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEK 203

[Close]

BLAST of Chy10G178540 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 136.0 bits (341), Expect = 3.7e-32
Identity = 67/174 (38.51%), Postives = 111/174 (63.79%), Query Frame = 0


Query: 49  ILNRLNKNGKKTDN-------IIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIY 108
           +L  LN+ GKK ++       ++  L++H+K    IS+    +LS G +++  GG  +++
Sbjct: 49  VLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAMARLSQGTKMIVEGGPERVF 108

Query: 109 KKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKISFCSDKSIKI-ASPNGDHIRIH 168
           ++ F +   EKLL    CY+STT+GP+ G+++IS  +I+FCSD +I++ +S  G+ +  +
Sbjct: 109 QREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFCSDYAIRLPSSAGGNGVAAY 168

Query: 169 YKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQE 215
           YKVV+  EK+  ++ S NV K SERY+ + T D FEFWFMGF++Y   FN L +
Sbjct: 169 YKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAFNCLNK 222

The following BLAST results are available for this feature:

BLAST of Chy10G178540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FTA0	1.2e-51	51.17	GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1	[more]
Q9FMW4	2.0e-51	49.77	Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV...	[more]
Q9FMW6	8.6e-50	59.24	GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2	[more]
Q9FMW5	5.6e-49	47.64	GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1	[more]
Q9M122	5.2e-31	38.51	GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1	[more]

BLAST of Chy10G178540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPM2	2.6e-102	96.08	GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579030 PE=3 S...	[more]
A0A1S3BEG1	2.6e-102	89.86	GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1	[more]
A0A5A7SSL2	1.1e-100	89.64	GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00...	[more]
A0A1S3CNX8	1.1e-100	89.64	GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1	[more]
A0A0A0KT32	1.1e-97	91.87	GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579000 PE=3 S...	[more]

BLAST of Chy10G178540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655497.2	1.31e-142	95.85	putative GEM-like protein 8 [Cucumis sativus]	[more]
XP_011655499.1	3.13e-135	91.44	GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572...	[more]
KGN52180.2	1.35e-132	96.08	hypothetical protein Csa_007871 [Cucumis sativus]	[more]
XP_008445894.1	4.31e-132	89.86	PREDICTED: GEM-like protein 4 [Cucumis melo]	[more]
XP_008465496.1	3.73e-130	89.64	PREDICTED: GEM-like protein 4 [Cucumis melo] >KAA0034082.1 GEM-like protein 4 [C...	[more]

BLAST of Chy10G178540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08350.1	8.5e-53	51.17	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	1.5e-52	49.77	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	6.1e-51	59.24	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	4.0e-50	47.64	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT4G01600.1	3.7e-32	38.51	GRAM domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	SMART	SM00568	gram2001c	coord: 99..177 e-value: 1.5E-16 score: 71.0
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 102..215 e-value: 1.7E-19 score: 69.8
IPR011993	PH-like domain superfamily	GENE3D	2.30.29.30		coord: 97..217 e-value: 6.5E-21 score: 76.2
None	No IPR available	PANTHER	PTHR31969:SF38	GEM-LIKE PROTEIN 4	coord: 1..215
IPR037848	GEM-like protein	PANTHER	PTHR31969	GEM-LIKE PROTEIN 2	coord: 1..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G178540.1	Chy10G178540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity