Chy10G178530 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G178530
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	GRAM domain-containing protein
Location	chrH10: 13082500 .. 13083512 (-)
RNA-Seq Expression	Chy10G178530
Synteny	Chy10G178530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGACTACAATTCAAGAGCAAATTGTTCTTGGAATTCAAATGAAGTCATTGGGTCTTGCAAGAGCTAGATTGTTGGGTGATCCCTCAAAATATCTCCATATATCTTCTTCAGACGATGGATCTCAATCAATTAAGAAATGTAAGACCTTATAAGAAGAAGGAAAAAGTACTGTTATTAAAAGTTGTTGAATGTTTAAATAATAGTTTGCTAAATGATTTGGTTTTTGAATTATTGTTGCAGATAATGGAAAGGATTGTATCTTGAATAGACTCAATAAAAATGGAAAGAAAACAGACAATATCATTCATGCGCTTCGAGAACACGGTAAGTCTTTTATATCGATTCAAGAATCTTTTTATCTACAATTTGAATTTAAAATTTGAACTTGATCATGCCACATGAAACTAAGACGATGTTATAGAGCGTATTTCTGATGATTTTGAGGTTGAATTTTGCAGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGCGTAAGGAAAATCTACAAGAAGTTATTTTTGATGCATGAAGGAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAACTGGTCCTTTAGCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTAGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTAATAAATTTAAACCCCTTCTTCTTTTTCAATTTAGTTTAGTTCTCTTTTTTTCCCTCATATACTAATGATTTCCAAAAATAAACGTATTTCTTTCAGGTTACAATTCCCCTGGGGAAAATAACAAGAGTTTTCCAAAGTGAAAATGTGAAGAATCCATCAGAGAAGTATATGGAGATAGTGACAGTGGATAACTATGAGTTTTGGTTCATGGGATTTTTGAATTATCACAAGTCATTCAATTGTCTTCAAGAAGCACTTTCTTCTCAAGCTTAG

mRNA sequence

ATGAAGACTACAATTCAAGAGCAAATTGTTCTTGGAATTCAAATGAAGTCATTGGGTCTTGCAAGAGCTAGATTGTTGGGTGATCCCTCAAAATATCTCCATATATCTTCTTCAGACGATGGATCTCAATCAATTAAGAAATATAATGGAAAGGATTGTATCTTGAATAGACTCAATAAAAATGGAAAGAAAACAGACAATATCATTCATGCGCTTCGAGAACACGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGCGTAAGGAAAATCTACAAGAAGTTATTTTTGATGCATGAAGGAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAACTGGTCCTTTAGCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTAGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTTACAATTCCCCTGGGGAAAATAACAAGAGTTTTCCAAAGTGAAAATGTGAAGAATCCATCAGAGAAGTATATGGAGATAGTGACAGTGGATAACTATGAGTTTTGGTTCATGGGATTTTTGAATTATCACAAGTCATTCAATTGTCTTCAAGAAGCACTTTCTTCTCAAGCTTAG

Coding sequence (CDS)

ATGAAGACTACAATTCAAGAGCAAATTGTTCTTGGAATTCAAATGAAGTCATTGGGTCTTGCAAGAGCTAGATTGTTGGGTGATCCCTCAAAATATCTCCATATATCTTCTTCAGACGATGGATCTCAATCAATTAAGAAATATAATGGAAAGGATTGTATCTTGAATAGACTCAATAAAAATGGAAAGAAAACAGACAATATCATTCATGCGCTTCGAGAACACGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGCGTAAGGAAAATCTACAAGAAGTTATTTTTGATGCATGAAGGAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAACTGGTCCTTTAGCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTAGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTTACAATTCCCCTGGGGAAAATAACAAGAGTTTTCCAAAGTGAAAATGTGAAGAATCCATCAGAGAAGTATATGGAGATAGTGACAGTGGATAACTATGAGTTTTGGTTCATGGGATTTTTGAATTATCACAAGTCATTCAATTGTCTTCAAGAAGCACTTTCTTCTCAAGCTTAG

Protein sequence

MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNKNGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA*

Homology

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BLAST of Chy10G178530 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 8.3e-56
Identity = 103/160 (64.38%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KKTD   + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF + EGEKL K+ 
Sbjct: 54  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 113

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT GP+AGLLFIS+ K+AF S++SIK+ SP GD IR+HYKV+IPL KI RV QS+
Sbjct: 114 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 173

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           N K PS+KY+E+VTVD ++FWFMGFL+Y K+FNCL++ALS
Sbjct: 174 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

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BLAST of Chy10G178530 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-53
Identity = 100/161 (62.11%), Postives = 132/161 (81.99%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + + EKL K  
Sbjct: 55  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 114

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT GP+AGLLFIS+ KIAF S++SIK+ASP G   R+HYKV+IPL KI  V QS+
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 174

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSS 224
           N K PS+KY+EIVT+DN++FWFMGF++Y K+FNCL++AL++
Sbjct: 175 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNN 215

[Close]

BLAST of Chy10G178530 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-53
Identity = 100/160 (62.50%), Postives = 130/160 (81.25%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KK D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF + + EKL K  
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT GP+AGLLFIS+ KIAF S++SIK+ASP G+  R+HYKV+IPL KI  V QS+
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           N   PS+KY+E+VTVD ++FWFMGFL+Y K+FNCL++ALS
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALS 214

[Close]

BLAST of Chy10G178530 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.0e-53
Identity = 99/160 (61.88%), Postives = 130/160 (81.25%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + + EKL K  
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT G +AGLLFIS+ KIAF S++SIK+ SP GD  R+HYKV+IPL KI  V QS+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           N K PS++Y+E+VTVDNY+FWFMGF++Y K+FNCL++AL+
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALN 206

[Close]

BLAST of Chy10G178530 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.6e-33
Identity = 75/198 (37.88%), Postives = 122/198 (61.62%), Query Frame = 0


Query: 32  YLHISSSDDGSQSIKKYNGKDCILNRLNKNGKKTDN-------IIHALREHVKLGAKISE 91
           Y+HI+S    S        KD +L  LN+ GKK ++       ++  L++H+K    IS+
Sbjct: 31  YVHITSPTSASDK----RSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISD 90

Query: 92  TVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVSQCYLSTTTGPLAGLLFISTHKI 151
               +LS G +++  GG  +++++ F +   EKLL    CY+STT+GP+ G+++IS  +I
Sbjct: 91  AAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRI 150

Query: 152 AFSSDKSIKI-ASPNGDHIRIHYKVTIPLGKITRVFQSENVKNPSEKYMEIVTVDNYEFW 211
           AF SD +I++ +S  G+ +  +YKV +   KI+ +  S NV  PSE+Y+ +VT D +EFW
Sbjct: 151 AFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFW 210

Query: 212 FMGFLNYHKSFNCLQEAL 222
           FMGF++Y  +FNCL +AL
Sbjct: 211 FMGFVSYIDAFNCLNKAL 224

[Close]

BLAST of Chy10G178530 vs. ExPASy TrEMBL
Match: A0A0A0KV22 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G578990 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.0e-117
Identity = 219/225 (97.33%), Postives = 219/225 (97.33%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLF M E EKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180
           VSQCYLSTT GPL GLLFISTHKIAF SDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180

Query: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA 226
           SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA
Sbjct: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA 225

[Close]

BLAST of Chy10G178530 vs. ExPASy TrEMBL
Match: A0A5A7SX89 (GEM-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00690 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.0e-114
Identity = 215/225 (95.56%), Postives = 218/225 (96.89%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQIVLGIQMKSLGLARARLLGDPSK+LHI  SDDGSQSIKKYNGKDCILNRLNK
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKHLHI--SDDGSQSIKKYNGKDCILNRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NGKKTDNII ALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLF M+E EKLLK
Sbjct: 61  NGKKTDNIIQALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180
           VSQCYLSTT GPLAGLLFISTHKIAF SDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ
Sbjct: 121 VSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180

Query: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA 226
           SENVKNPSEKYMEIVT+DNYEFWFMGFLNYHKSFNCLQEALSSQA
Sbjct: 181 SENVKNPSEKYMEIVTLDNYEFWFMGFLNYHKSFNCLQEALSSQA 223

[Close]

BLAST of Chy10G178530 vs. ExPASy TrEMBL
Match: A0A1S3CPR8 (GEM-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103503290 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.0e-114
Identity = 215/225 (95.56%), Postives = 218/225 (96.89%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQIVLGIQMKSLGLARARLLGDPSK+LHI  SDDGSQSIKKYNGKDCILNRLNK
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKHLHI--SDDGSQSIKKYNGKDCILNRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NGKKTDNII ALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLF M+E EKLLK
Sbjct: 61  NGKKTDNIIQALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180
           VSQCYLSTT GPLAGLLFISTHKIAF SDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ
Sbjct: 121 VSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180

Query: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA 226
           SENVKNPSEKYMEIVT+DNYEFWFMGFLNYHKSFNCLQEALSSQA
Sbjct: 181 SENVKNPSEKYMEIVTLDNYEFWFMGFLNYHKSFNCLQEALSSQA 223

[Close]

BLAST of Chy10G178530 vs. ExPASy TrEMBL
Match: A0A6J1GW39 (putative GEM-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111458124 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.8e-101
Identity = 190/223 (85.20%), Postives = 208/223 (93.27%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQ VLG QMKSL LARAR L DPSK+LHISSSDDGSQSIKKYNGKD ILNRL+K
Sbjct: 1   MKTTIQEQ-VLGAQMKSLALARARFLADPSKHLHISSSDDGSQSIKKYNGKDWILNRLHK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NG+KTDNIIHALR+HVKLGAKI+ETVKGKLSLGARI+RVGGVRKIYKKLF + EGEKLLK
Sbjct: 61  NGRKTDNIIHALRQHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNG-DHIRIHYKVTIPLGKITRVF 180
            SQCYLSTT GP+AGLLFISTH IAF SDKSIK++SPNG +H+RIHYKVTIPLGKI RVF
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVTIPLGKIRRVF 180

Query: 181 QSENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           +SENVKNP EKY+E+VTVD++EFWFMGFLNYHK+FNCLQEALS
Sbjct: 181 ESENVKNPWEKYIEMVTVDDFEFWFMGFLNYHKTFNCLQEALS 222

[Close]

BLAST of Chy10G178530 vs. ExPASy TrEMBL
Match: A0A6J1KB00 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493833 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.4e-98
Identity = 187/223 (83.86%), Postives = 205/223 (91.93%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQ VLG QMKSL LARAR L DPSK+LHISSSDDGSQSIKKYNGKD  LNRL+K
Sbjct: 1   MKTTIQEQ-VLGAQMKSLALARARFLADPSKHLHISSSDDGSQSIKKYNGKDWSLNRLHK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NG+KTDNIIHALR+HVKLGAKI+ETVKGKLSLGARI+RVGGVRKIYKKLF + EGEKLLK
Sbjct: 61  NGRKTDNIIHALRQHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGD-HIRIHYKVTIPLGKITRVF 180
            SQCYLSTT GP+AGLLFISTH IAF SDKSIK++SPNG+ H+RIHYKV IPLGKI RVF
Sbjct: 121 ASQCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLSSPNGEKHVRIHYKVKIPLGKIRRVF 180

Query: 181 QSENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           +SENVKNP EKY+E+VTVD++EFWFMGFLNY K+FNCLQEALS
Sbjct: 181 ESENVKNPWEKYIEMVTVDDFEFWFMGFLNYQKTFNCLQEALS 222

[Close]

BLAST of Chy10G178530 vs. NCBI nr
Match: XP_011655500.1 (GEM-like protein 7 [Cucumis sativus] >KGN51561.1 hypothetical protein Csa_008634 [Cucumis sativus])

HSP 1 Score: 427 bits (1099), Expect = 1.37e-150
Identity = 219/225 (97.33%), Postives = 219/225 (97.33%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLF M E EKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180
           VSQCYLSTT GPL GLLFISTHKIAF SDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180

Query: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA 225
           SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA
Sbjct: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA 225

[Close]

BLAST of Chy10G178530 vs. NCBI nr
Match: XP_008465648.1 (PREDICTED: GEM-like protein 7 [Cucumis melo] >KAA0034081.1 GEM-like protein 7 [Cucumis melo var. makuwa])

HSP 1 Score: 416 bits (1069), Expect = 4.76e-146
Identity = 215/225 (95.56%), Postives = 218/225 (96.89%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQIVLGIQMKSLGLARARLLGDPSK+LHIS  DDGSQSIKKYNGKDCILNRLNK
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKHLHIS--DDGSQSIKKYNGKDCILNRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NGKKTDNII ALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLF M+E EKLLK
Sbjct: 61  NGKKTDNIIQALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180
           VSQCYLSTT GPLAGLLFISTHKIAF SDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ
Sbjct: 121 VSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180

Query: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQA 225
           SENVKNPSEKYMEIVT+DNYEFWFMGFLNYHKSFNCLQEALSSQA
Sbjct: 181 SENVKNPSEKYMEIVTLDNYEFWFMGFLNYHKSFNCLQEALSSQA 223

[Close]

BLAST of Chy10G178530 vs. NCBI nr
Match: XP_038892955.1 (GEM-like protein 7 [Benincasa hispida])

HSP 1 Score: 404 bits (1039), Expect = 1.91e-141
Identity = 203/224 (90.62%), Postives = 215/224 (95.98%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQ++LGIQMKSLGLARARLLGDPSK+LHISSSDDGSQSIKKYNGKD +LNRLNK
Sbjct: 1   MKTTIQEQVILGIQMKSLGLARARLLGDPSKHLHISSSDDGSQSIKKYNGKDWVLNRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGG+RKIYKKLF M EGEKL+K
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSMTEGEKLIK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180
            SQCYLSTT GPLAGLLFIS+HKIAF SDKSIK++S NGDHIRIHYKVTIPLGKI  VFQ
Sbjct: 121 ASQCYLSTTAGPLAGLLFISSHKIAFCSDKSIKLSSQNGDHIRIHYKVTIPLGKIKTVFQ 180

Query: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSSQ 224
           SENVKNPSEKYMEIVTVD++EFWFMGFLNYHKSFNC+QEALSSQ
Sbjct: 181 SENVKNPSEKYMEIVTVDDFEFWFMGFLNYHKSFNCIQEALSSQ 224

[Close]

BLAST of Chy10G178530 vs. NCBI nr
Match: XP_022956362.1 (putative GEM-like protein 8 [Cucurbita moschata] >KAG6601068.1 GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 373 bits (958), Expect = 4.07e-129
Identity = 190/223 (85.20%), Postives = 208/223 (93.27%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQ VLG QMKSL LARAR L DPSK+LHISSSDDGSQSIKKYNGKD ILNRL+K
Sbjct: 1   MKTTIQEQ-VLGAQMKSLALARARFLADPSKHLHISSSDDGSQSIKKYNGKDWILNRLHK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NG+KTDNIIHALR+HVKLGAKI+ETVKGKLSLGARI+RVGGVRKIYKKLF + EGEKLLK
Sbjct: 61  NGRKTDNIIHALRQHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGD-HIRIHYKVTIPLGKITRVF 180
            SQCYLSTT GP+AGLLFISTH IAF SDKSIK++SPNG+ H+RIHYKVTIPLGKI RVF
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVTIPLGKIRRVF 180

Query: 181 QSENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 222
           +SENVKNP EKY+E+VTVD++EFWFMGFLNYHK+FNCLQEALS
Sbjct: 181 ESENVKNPWEKYIEMVTVDDFEFWFMGFLNYHKTFNCLQEALS 222

[Close]

BLAST of Chy10G178530 vs. NCBI nr
Match: XP_023513103.1 (putative GEM-like protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372 bits (954), Expect = 1.65e-128
Identity = 189/223 (84.75%), Postives = 207/223 (92.83%), Query Frame = 0


Query: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60
           MKTTIQEQ VLG QMKSL LARAR L DPSK+LHISSSDDGSQSIKKYNGKD ILNRL+K
Sbjct: 1   MKTTIQEQ-VLGAQMKSLALARARFLADPSKHLHISSSDDGSQSIKKYNGKDWILNRLHK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLK 120
           NG+KTDNIIHALR+HVKLGAKI+ETVKGKLSLGARI+RVGGVRKIYKKLF + EGEKLLK
Sbjct: 61  NGRKTDNIIHALRQHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 VSQCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGD-HIRIHYKVTIPLGKITRVF 180
            SQCYLSTT GP+AGLLFISTH IAF SDKSIK++SPNG+ H+RIHYKVTIPLGKI RVF
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVTIPLGKIRRVF 180

Query: 181 QSENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 222
           +SENVKNP EKY+E+VTVD++EFWFMGF NYHK+FNCLQEALS
Sbjct: 181 ESENVKNPWEKYIEMVTVDDFEFWFMGFFNYHKTFNCLQEALS 222

[Close]

BLAST of Chy10G178530 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 218.4 bits (555), Expect = 5.9e-57
Identity = 103/160 (64.38%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KKTD   + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF + EGEKL K+ 
Sbjct: 54  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 113

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT GP+AGLLFIS+ K+AF S++SIK+ SP GD IR+HYKV+IPL KI RV QS+
Sbjct: 114 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 173

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           N K PS+KY+E+VTVD ++FWFMGFL+Y K+FNCL++ALS
Sbjct: 174 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

[Close]

BLAST of Chy10G178530 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 211.1 bits (536), Expect = 9.4e-55
Identity = 100/160 (62.50%), Postives = 130/160 (81.25%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KK D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF + + EKL K  
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT GP+AGLLFIS+ KIAF S++SIK+ASP G+  R+HYKV+IPL KI  V QS+
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           N   PS+KY+E+VTVD ++FWFMGFL+Y K+FNCL++ALS
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALS 214

[Close]

BLAST of Chy10G178530 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 211.1 bits (536), Expect = 9.4e-55
Identity = 100/161 (62.11%), Postives = 132/161 (81.99%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + + EKL K  
Sbjct: 117 KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 176

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT GP+AGLLFIS+ KIAF S++SIK+ASP G   R+HYKV+IPL KI  V QS+
Sbjct: 177 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 236

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALSS 224
           N K PS+KY+EIVT+DN++FWFMGF++Y K+FNCL++AL++
Sbjct: 237 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNN 277

[Close]

BLAST of Chy10G178530 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 209.9 bits (533), Expect = 2.1e-54
Identity = 99/160 (61.88%), Postives = 130/160 (81.25%), Query Frame = 0


Query: 63  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVS 122
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + + EKL K  
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 123 QCYLSTTTGPLAGLLFISTHKIAFSSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQSE 182
           QCYLSTT G +AGLLFIS+ KIAF S++SIK+ SP GD  R+HYKV+IPL KI  V QS+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 183 NVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQEALS 223
           N K PS++Y+E+VTVDNY+FWFMGF++Y K+FNCL++AL+
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALN 206

[Close]

BLAST of Chy10G178530 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.9e-34
Identity = 75/198 (37.88%), Postives = 122/198 (61.62%), Query Frame = 0


Query: 32  YLHISSSDDGSQSIKKYNGKDCILNRLNKNGKKTDN-------IIHALREHVKLGAKISE 91
           Y+HI+S    S        KD +L  LN+ GKK ++       ++  L++H+K    IS+
Sbjct: 31  YVHITSPTSASDK----RSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISD 90

Query: 92  TVKGKLSLGARILRVGGVRKIYKKLFLMHEGEKLLKVSQCYLSTTTGPLAGLLFISTHKI 151
               +LS G +++  GG  +++++ F +   EKLL    CY+STT+GP+ G+++IS  +I
Sbjct: 91  AAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRI 150

Query: 152 AFSSDKSIKI-ASPNGDHIRIHYKVTIPLGKITRVFQSENVKNPSEKYMEIVTVDNYEFW 211
           AF SD +I++ +S  G+ +  +YKV +   KI+ +  S NV  PSE+Y+ +VT D +EFW
Sbjct: 151 AFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFW 210

Query: 212 FMGFLNYHKSFNCLQEAL 222
           FMGF++Y  +FNCL +AL
Sbjct: 211 FMGFVSYIDAFNCLNKAL 224

The following BLAST results are available for this feature:

BLAST of Chy10G178530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FTA0	8.3e-56	64.38	GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1	[more]
Q9FMW6	1.3e-53	62.11	GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2	[more]
Q9FMW4	1.3e-53	62.50	Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV...	[more]
Q9FMW5	3.0e-53	61.88	GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1	[more]
Q9M122	2.6e-33	37.88	GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1	[more]

BLAST of Chy10G178530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV22	3.0e-117	97.33	GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G578990 PE=3 S...	[more]
A0A5A7SX89	7.0e-114	95.56	GEM-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00...	[more]
A0A1S3CPR8	7.0e-114	95.56	GEM-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103503290 PE=3 SV=1	[more]
A0A6J1GW39	6.8e-101	85.20	putative GEM-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111458124 PE=3 S...	[more]
A0A6J1KB00	2.4e-98	83.86	putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493833 PE=3 SV=...	[more]

BLAST of Chy10G178530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655500.1	1.37e-150	97.33	GEM-like protein 7 [Cucumis sativus] >KGN51561.1 hypothetical protein Csa_008634...	[more]
XP_008465648.1	4.76e-146	95.56	PREDICTED: GEM-like protein 7 [Cucumis melo] >KAA0034081.1 GEM-like protein 7 [C...	[more]
XP_038892955.1	1.91e-141	90.63	GEM-like protein 7 [Benincasa hispida]	[more]
XP_022956362.1	4.07e-129	85.20	putative GEM-like protein 8 [Cucurbita moschata] >KAG6601068.1 GEM-like protein ...	[more]
XP_023513103.1	1.65e-128	84.75	putative GEM-like protein 8 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy10G178530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08350.1	5.9e-57	64.38	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	9.4e-55	62.50	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	9.4e-55	62.11	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	2.1e-54	61.88	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT4G01600.1	1.9e-34	37.88	GRAM domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	SMART	SM00568	gram2001c	coord: 104..182 e-value: 1.4E-17 score: 74.4
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 107..223 e-value: 2.0E-19 score: 69.6
IPR011993	PH-like domain superfamily	GENE3D	2.30.29.30		coord: 102..223 e-value: 4.1E-19 score: 70.4
None	No IPR available	PANTHER	PTHR31969:SF38	GEM-LIKE PROTEIN 4	coord: 1..224
IPR037848	GEM-like protein	PANTHER	PTHR31969	GEM-LIKE PROTEIN 2	coord: 1..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G178530.1	Chy10G178530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity