Chy10G178520 (gene) Cucumber (hystrix) v1

Overview
NameChy10G178520
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionGRAM domain-containing protein
LocationchrH10: 13075297 .. 13076280 (-)
RNA-Seq ExpressionChy10G178520
SyntenyChy10G178520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAGAGAGTTTGGAAGAAGAGGTTACAGCTTTGTTAATGGAATCCTTGAACATGGTAAACATAAATTGCTTTCTTCTTTCCTGTCTGACTATGATCATGAAATGATGGATTGGATTGAATTGTCCTCTTTGTTTTTCTAGAAACTTCTGTTTCTCATCTTGTTTTCTATAAATCCAATGCATAATGCCCCGAAATCCCTCCGTCGGTCTAAACATGATTCAACTAGAGAAACCGAAAGCACTATTTATCTCTTCCTACGTAGATAGAGATTGGCATTGATTCTTCCTGCATTGTTAACAATGTATAAATATACTTGATTCGAGGGATTAAAAATGCTTGACGGTTGCAATGGAATGATGACGTGCAGTTAAACTGTCACCGAACATAGCCAAAACGGTGAAGGGGAAGCTGTGGTTGGGGACAAAACTTTTACAGTTTGGAGGAAGTGAGAATATATTTCACAAGATGTTCAATCTCGAACAAGGAGACAAGTTATTGAATTCTGCACATTGTTATTTGTCAACAACAGCAGGTCCTATAGCAGGTCTCGTCTTTGTTTCCACCCATGTAGTTGCTTTCTGCAGTGACAGACCAATCATCATTTCTTCTCCACATGGAGAAATTGGCAAAATATTTTACAAAGTAAATAATATTTCCTTTCTTCAACATTTACATTTGACTATTTCCCTCACTCTCTCTTTCTTCCTGGTTGCTAACCAAGTTTTTGTTTTCTATAATAATGTTTGTCCCATCAGGTTATGATCCCCGTGAACAAAGTGAAGAGAGTTAACCAAAGAAACAATGTAAACAATCCAGCAAAGAAATACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATCATGAGAAAGCGTTTAAGTTCATACAAAAGGGTGTTTCTATGGATTCAGAAGATTGGCCTGAAGAGGCCCAAAGAAAGTGTCGTCCAGGAAAGTTCTAG

mRNA sequence

ATGATCAGAGAGTTTGGAAGAAGAGGTTACAGCTTTGTTAATGGAATCCTTGAACATGTTAAACTGTCACCGAACATAGCCAAAACGGTGAAGGGGAAGCTGTGGTTGGGGACAAAACTTTTACAGTTTGGAGGAAGTGAGAATATATTTCACAAGATGTTCAATCTCGAACAAGGAGACAAGTTATTGAATTCTGCACATTGTTATTTGTCAACAACAGCAGGTCCTATAGCAGGTCTCGTCTTTGTTTCCACCCATGTAGTTGCTTTCTGCAGTGACAGACCAATCATCATTTCTTCTCCACATGGAGAAATTGGCAAAATATTTTACAAAGTTATGATCCCCGTGAACAAAGTGAAGAGAGTTAACCAAAGAAACAATGTAAACAATCCAGCAAAGAAATACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATCATGAGAAAGCGTTTAAGTTCATACAAAAGGGTGTTTCTATGGATTCAGAAGATTGGCCTGAAGAGGCCCAAAGAAAGTGTCGTCCAGGAAAGTTCTAG

Coding sequence (CDS)

ATGATCAGAGAGTTTGGAAGAAGAGGTTACAGCTTTGTTAATGGAATCCTTGAACATGTTAAACTGTCACCGAACATAGCCAAAACGGTGAAGGGGAAGCTGTGGTTGGGGACAAAACTTTTACAGTTTGGAGGAAGTGAGAATATATTTCACAAGATGTTCAATCTCGAACAAGGAGACAAGTTATTGAATTCTGCACATTGTTATTTGTCAACAACAGCAGGTCCTATAGCAGGTCTCGTCTTTGTTTCCACCCATGTAGTTGCTTTCTGCAGTGACAGACCAATCATCATTTCTTCTCCACATGGAGAAATTGGCAAAATATTTTACAAAGTTATGATCCCCGTGAACAAAGTGAAGAGAGTTAACCAAAGAAACAATGTAAACAATCCAGCAAAGAAATACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATCATGAGAAAGCGTTTAAGTTCATACAAAAGGGTGTTTCTATGGATTCAGAAGATTGGCCTGAAGAGGCCCAAAGAAAGTGTCGTCCAGGAAAGTTCTAG

Protein sequence

MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSEDWPEEAQRKCRPGKF*
Homology
BLAST of Chy10G178520 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.8e-46
Identity = 84/164 (51.22%), Postives = 119/164 (72.56%), Query Frame = 0

Query: 12  FVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYLS 71
           F NG+ +  K+ P + +TVK KL LG ++LQ GG E IF ++F + +G+KL     CYLS
Sbjct: 59  FTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLS 118

Query: 72  TTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNP 131
           TTAGPIAGL+F+S+  +AFCS+R I + SP G+I ++ YKV IP+ K+ RVNQ  N   P
Sbjct: 119 TTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKP 178

Query: 132 AKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSEDWPEE 176
           ++KY++VVTVD FDFWFMGFL+++KAF  ++K +S+  ED  E+
Sbjct: 179 SQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALSLSFEDNKEQ 222

BLAST of Chy10G178520 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.1e-44
Identity = 79/161 (49.07%), Postives = 117/161 (72.67%), Query Frame = 0

Query: 7   RRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSA 66
           ++  SF NG+ +  KL P + +TVK KL LG ++LQ GG E I+ ++F +   +KL  + 
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 67  HCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRN 126
            CYLSTTAGPIAGL+F+S+  +AFCS+R I ++SP GE+ ++ YKV IP+ K+  VNQ  
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 127 NVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSM 168
           N   P++KY++VVTVD FDFWFMGFL+++KAF  +++ +S+
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSL 215

BLAST of Chy10G178520 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 176.0 bits (445), Expect = 3.9e-43
Identity = 77/160 (48.12%), Postives = 115/160 (71.88%), Query Frame = 0

Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 59  SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYL 118

Query: 71  STTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STTAGPIAGL+F+S+  +AFCS+R I ++SP G + ++ YKV IP+ K+  VNQ  N   
Sbjct: 119 STTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKK 178

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSE 171
           P++KY+++VT+D+FDFWFMGF++++KAF  ++K ++ D +
Sbjct: 179 PSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNNDEQ 218

BLAST of Chy10G178520 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.1e-41
Identity = 75/160 (46.88%), Postives = 114/160 (71.25%), Query Frame = 0

Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 51  SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYL 110

Query: 71  STTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STT G IAGL+F+S+  +AFCS+R I ++SP G++ ++ YKV IP+ K+  VNQ  N   
Sbjct: 111 STTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKK 170

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSE 171
           P+++Y++VVTVD++DFWFMGF++++KAF  ++K ++ D +
Sbjct: 171 PSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNEDEQ 210

BLAST of Chy10G178520 vs. ExPASy Swiss-Prot
Match: Q9LYV6 (GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 5.8e-31
Identity = 65/166 (39.16%), Postives = 100/166 (60.24%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           M   + R+  +    +  ++K  P++++T  GK+ L  K +  GG E++F ++F  E  +
Sbjct: 96  MFHTWSRKAETVARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNE 155

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
            L  +  CYLSTT GP+AG V++S   VAFCSDRP+  ++P G+    +Y+V++P+  V 
Sbjct: 156 TLKKTFACYLSTTTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVA 215

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVS 167
            VN       P +KYIQ+ TVD  DFWFMGF+N+EKA   +   VS
Sbjct: 216 TVNPVVVKETPPEKYIQLTTVDGHDFWFMGFVNYEKATHHLLTSVS 261

BLAST of Chy10G178520 vs. ExPASy TrEMBL
Match: A0A0A0KPM0 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G577990 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.2e-100
Identity = 179/185 (96.76%), Postives = 181/185 (97.84%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MIRE GRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLE GD
Sbjct: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLLNSAHCYLSTTAGPIAGL+FVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSEDWPEEAQRKC 180
           RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQ GVS+DSEDWPEEAQRKC
Sbjct: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC 180

Query: 181 RPGKF 186
            PGKF
Sbjct: 181 CPGKF 185

BLAST of Chy10G178520 vs. ExPASy TrEMBL
Match: A0A1S3BDR8 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488777 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.1e-88
Identity = 159/168 (94.64%), Postives = 164/168 (97.62%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MIRE GR+GYSFVNGILEHVKLSPNIAKTVKGKLWLGTKL+QFGGSE IFHKMFNLEQGD
Sbjct: 1   MIRELGRKGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGE+GK+FYKVMIPVNKVK
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMD 169
           RVNQRNN NNPAKKYIQVVTVDDFDFWFMGFLN+EK FKFIQKGVSMD
Sbjct: 121 RVNQRNNENNPAKKYIQVVTVDDFDFWFMGFLNYEKTFKFIQKGVSMD 168

BLAST of Chy10G178520 vs. ExPASy TrEMBL
Match: A0A5A7SSY1 (GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00680 PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 4.3e-77
Identity = 142/149 (95.30%), Postives = 146/149 (97.99%), Query Frame = 0

Query: 20  VKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 79
           VKLSPNIAKTVKGKLWLGTKL+QFGGSE IFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG
Sbjct: 6   VKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 65

Query: 80  LVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNPAKKYIQVV 139
           LVFVSTHVVAFCSDRPIIISSPHGE+GK+FYKVMIPVNKVKRVNQRNN NNPAKKYIQVV
Sbjct: 66  LVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVKRVNQRNNENNPAKKYIQVV 125

Query: 140 TVDDFDFWFMGFLNHEKAFKFIQKGVSMD 169
           TVDDFDFWFMGFLN+EK FKFIQKGVSMD
Sbjct: 126 TVDDFDFWFMGFLNYEKTFKFIQKGVSMD 154

BLAST of Chy10G178520 vs. ExPASy TrEMBL
Match: A0A6J1CD83 (putative GEM-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111010135 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.2e-71
Identity = 131/168 (77.98%), Postives = 151/168 (89.88%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MIR+ GR+  SFVNGIL+HVKLSPN+A+TVKGKLWL  KL+QFGGS  IF K+FNL QGD
Sbjct: 4   MIRKLGRKADSFVNGILDHVKLSPNVAETVKGKLWLVAKLVQFGGSGKIFQKLFNLRQGD 63

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLL+S+HCYLSTTAGPIAGL+F+ST+ VAFCSDRPIII+SPHGE+G+  YKVMIPV K+K
Sbjct: 64  KLLSSSHCYLSTTAGPIAGLLFISTNAVAFCSDRPIIITSPHGELGRFNYKVMIPVKKMK 123

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMD 169
           RVNQ  NV+NP+KKYIQVVTVDDFDFWFMGFLN+ KAFKF+QKGVS D
Sbjct: 124 RVNQTENVSNPSKKYIQVVTVDDFDFWFMGFLNYGKAFKFLQKGVSED 171

BLAST of Chy10G178520 vs. ExPASy TrEMBL
Match: A0A6J1HVV6 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111466633 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.6e-63
Identity = 119/168 (70.83%), Postives = 139/168 (82.74%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MIR  G +   FVNG+ +HVKLSP++ KTVK KLWLG KL+Q G SE IF KMF L Q D
Sbjct: 1   MIRNIGSKAARFVNGVFQHVKLSPSVTKTVKRKLWLGAKLVQIGRSEKIFKKMFKLRQDD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLL+S+HCYLSTTAGPIAGL+FVSTHVVAFCSDRPII SSPH E  ++ YKVMIP+ K+K
Sbjct: 61  KLLSSSHCYLSTTAGPIAGLLFVSTHVVAFCSDRPIIFSSPHEEQSRMNYKVMIPMKKMK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMD 169
           RVN+  NVNNP+KKYIQVVTVD F+FWFMGFLN++ AFKF+ KG+S D
Sbjct: 121 RVNESENVNNPSKKYIQVVTVDGFEFWFMGFLNYKIAFKFLPKGLSKD 168

BLAST of Chy10G178520 vs. NCBI nr
Match: XP_004147134.1 (GEM-like protein 4 [Cucumis sativus] >KGN51560.1 hypothetical protein Csa_008945 [Cucumis sativus])

HSP 1 Score: 375 bits (962), Expect = 5.52e-131
Identity = 179/185 (96.76%), Postives = 181/185 (97.84%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MIRE GRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLE GD
Sbjct: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLLNSAHCYLSTTAGPIAGL+FVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSEDWPEEAQRKC 180
           RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQ GVS+DSEDWPEEAQRKC
Sbjct: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVDSEDWPEEAQRKC 180

Query: 181 RPGKF 185
            PGKF
Sbjct: 181 CPGKF 185

BLAST of Chy10G178520 vs. NCBI nr
Match: XP_008445893.1 (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 333 bits (853), Expect = 1.14e-114
Identity = 159/168 (94.64%), Postives = 164/168 (97.62%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MIRE GR+GYSFVNGILEHVKLSPNIAKTVKGKLWLGTKL+QFGGSE IFHKMFNLEQGD
Sbjct: 1   MIRELGRKGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGE+GK+FYKVMIPVNKVK
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVK 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMD 168
           RVNQRNN NNPAKKYIQVVTVDDFDFWFMGFLN+EK FKFIQKGVSMD
Sbjct: 121 RVNQRNNENNPAKKYIQVVTVDDFDFWFMGFLNYEKTFKFIQKGVSMD 168

BLAST of Chy10G178520 vs. NCBI nr
Match: XP_038893009.1 (GEM-like protein 4 [Benincasa hispida])

HSP 1 Score: 298 bits (762), Expect = 8.48e-101
Identity = 144/165 (87.27%), Postives = 156/165 (94.55%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MI E GRRGY FVNGILEHVKLSPNIAKTVKGKLWLGTKL+QFG SE IFHKMFNL+QGD
Sbjct: 1   MIGELGRRGYRFVNGILEHVKLSPNIAKTVKGKLWLGTKLVQFG-SEKIFHKMFNLKQGD 60

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLL+SAHCYLST+AGPIAGL+FVSTHVVAFCSDRPIIISSP GE+G++FYKVMIPV KV 
Sbjct: 61  KLLSSAHCYLSTSAGPIAGLLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVG 120

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGV 165
           RVNQRNNV+NP+KKYIQVVTVD FDFWFMGFLN+EKAFKF+QKGV
Sbjct: 121 RVNQRNNVSNPSKKYIQVVTVDGFDFWFMGFLNYEKAFKFLQKGV 164

BLAST of Chy10G178520 vs. NCBI nr
Match: KAA0034080.1 (GEM-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 296 bits (759), Expect = 1.50e-100
Identity = 142/149 (95.30%), Postives = 146/149 (97.99%), Query Frame = 0

Query: 20  VKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 79
           VKLSPNIAKTVKGKLWLGTKL+QFGGSE IFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG
Sbjct: 6   VKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 65

Query: 80  LVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNPAKKYIQVV 139
           LVFVSTHVVAFCSDRPIIISSPHGE+GK+FYKVMIPVNKVKRVNQRNN NNPAKKYIQVV
Sbjct: 66  LVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVKRVNQRNNENNPAKKYIQVV 125

Query: 140 TVDDFDFWFMGFLNHEKAFKFIQKGVSMD 168
           TVDDFDFWFMGFLN+EK FKFIQKGVSMD
Sbjct: 126 TVDDFDFWFMGFLNYEKTFKFIQKGVSMD 154

BLAST of Chy10G178520 vs. NCBI nr
Match: XP_022139162.1 (putative GEM-like protein 8 [Momordica charantia])

HSP 1 Score: 278 bits (711), Expect = 5.55e-93
Identity = 131/168 (77.98%), Postives = 151/168 (89.88%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           MIR+ GR+  SFVNGIL+HVKLSPN+A+TVKGKLWL  KL+QFGGS  IF K+FNL QGD
Sbjct: 4   MIRKLGRKADSFVNGILDHVKLSPNVAETVKGKLWLVAKLVQFGGSGKIFQKLFNLRQGD 63

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
           KLL+S+HCYLSTTAGPIAGL+F+ST+ VAFCSDRPIII+SPHGE+G+  YKVMIPV K+K
Sbjct: 64  KLLSSSHCYLSTTAGPIAGLLFISTNAVAFCSDRPIIITSPHGELGRFNYKVMIPVKKMK 123

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMD 168
           RVNQ  NV+NP+KKYIQVVTVDDFDFWFMGFLN+ KAFKF+QKGVS D
Sbjct: 124 RVNQTENVSNPSKKYIQVVTVDDFDFWFMGFLNYGKAFKFLQKGVSED 171

BLAST of Chy10G178520 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 186.0 bits (471), Expect = 2.7e-47
Identity = 84/164 (51.22%), Postives = 119/164 (72.56%), Query Frame = 0

Query: 12  FVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYLS 71
           F NG+ +  K+ P + +TVK KL LG ++LQ GG E IF ++F + +G+KL     CYLS
Sbjct: 59  FTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLS 118

Query: 72  TTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNP 131
           TTAGPIAGL+F+S+  +AFCS+R I + SP G+I ++ YKV IP+ K+ RVNQ  N   P
Sbjct: 119 TTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKP 178

Query: 132 AKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSEDWPEE 176
           ++KY++VVTVD FDFWFMGFL+++KAF  ++K +S+  ED  E+
Sbjct: 179 SQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALSLSFEDNKEQ 222

BLAST of Chy10G178520 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 180.3 bits (456), Expect = 1.5e-45
Identity = 79/161 (49.07%), Postives = 117/161 (72.67%), Query Frame = 0

Query: 7   RRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSA 66
           ++  SF NG+ +  KL P + +TVK KL LG ++LQ GG E I+ ++F +   +KL  + 
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 67  HCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRN 126
            CYLSTTAGPIAGL+F+S+  +AFCS+R I ++SP GE+ ++ YKV IP+ K+  VNQ  
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 127 NVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSM 168
           N   P++KY++VVTVD FDFWFMGFL+++KAF  +++ +S+
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSL 215

BLAST of Chy10G178520 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 176.0 bits (445), Expect = 2.8e-44
Identity = 77/160 (48.12%), Postives = 115/160 (71.88%), Query Frame = 0

Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 121 SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYL 180

Query: 71  STTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STTAGPIAGL+F+S+  +AFCS+R I ++SP G + ++ YKV IP+ K+  VNQ  N   
Sbjct: 181 STTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKK 240

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSE 171
           P++KY+++VT+D+FDFWFMGF++++KAF  ++K ++ D +
Sbjct: 241 PSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNNDEQ 280

BLAST of Chy10G178520 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 170.2 bits (430), Expect = 1.5e-42
Identity = 75/160 (46.88%), Postives = 114/160 (71.25%), Query Frame = 0

Query: 11  SFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGDKLLNSAHCYL 70
           SF NG  +  KL P + +TVK KL LG K+LQ GG E I+ ++F +   +KL  +  CYL
Sbjct: 51  SFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYL 110

Query: 71  STTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNN 130
           STT G IAGL+F+S+  +AFCS+R I ++SP G++ ++ YKV IP+ K+  VNQ  N   
Sbjct: 111 STTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKK 170

Query: 131 PAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVSMDSE 171
           P+++Y++VVTVD++DFWFMGF++++KAF  ++K ++ D +
Sbjct: 171 PSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNEDEQ 210

BLAST of Chy10G178520 vs. TAIR 10
Match: AT5G13200.1 (GRAM domain family protein )

HSP 1 Score: 135.6 bits (340), Expect = 4.1e-32
Identity = 65/166 (39.16%), Postives = 100/166 (60.24%), Query Frame = 0

Query: 1   MIREFGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEQGD 60
           M   + R+  +    +  ++K  P++++T  GK+ L  K +  GG E++F ++F  E  +
Sbjct: 96  MFHTWSRKAETVARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNE 155

Query: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120
            L  +  CYLSTT GP+AG V++S   VAFCSDRP+  ++P G+    +Y+V++P+  V 
Sbjct: 156 TLKKTFACYLSTTTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVA 215

Query: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQKGVS 167
            VN       P +KYIQ+ TVD  DFWFMGF+N+EKA   +   VS
Sbjct: 216 TVNPVVVKETPPEKYIQLTTVDGHDFWFMGFVNYEKATHHLLTSVS 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FTA03.8e-4651.22GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1[more]
Q9FMW42.1e-4449.07Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV... [more]
Q9FMW63.9e-4348.13GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2[more]
Q9FMW52.1e-4146.88GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1[more]
Q9LYV65.8e-3139.16GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KPM01.2e-10096.76GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G577990 PE=3 S... [more]
A0A1S3BDR84.1e-8894.64GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488777 PE=3 SV=1[more]
A0A5A7SSY14.3e-7795.30GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00... [more]
A0A6J1CD831.2e-7177.98putative GEM-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111010135 PE=3 ... [more]
A0A6J1HVV61.6e-6370.83putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111466633 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_004147134.15.52e-13196.76GEM-like protein 4 [Cucumis sativus] >KGN51560.1 hypothetical protein Csa_008945... [more]
XP_008445893.11.14e-11494.64PREDICTED: GEM-like protein 4 [Cucumis melo][more]
XP_038893009.18.48e-10187.27GEM-like protein 4 [Benincasa hispida][more]
KAA0034080.11.50e-10095.30GEM-like protein 4 [Cucumis melo var. makuwa][more]
XP_022139162.15.55e-9377.98putative GEM-like protein 8 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G08350.12.7e-4751.22GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23370.11.5e-4549.07GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23350.12.8e-4448.13GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23360.11.5e-4246.88GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G13200.14.1e-3239.16GRAM domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 48..126
e-value: 1.8E-15
score: 67.4
IPR004182GRAM domainPFAMPF02893GRAMcoord: 49..163
e-value: 3.6E-20
score: 72.0
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 45..166
e-value: 4.3E-21
score: 76.8
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 3..167
NoneNo IPR availablePANTHERPTHR31969:SF38GEM-LIKE PROTEIN 4coord: 3..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G178520.1Chy10G178520.1mRNA