Chy10G178390 (gene) Cucumber (hystrix) v1

Overview
NameChy10G178390
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionJ domain-containing protein
LocationchrH10: 12997797 .. 12999467 (-)
RNA-Seq ExpressionChy10G178390
SyntenyChy10G178390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGCCTCATGGCTTCCTCTGTACACACCCACAACACAATATCCTATTCAAAGAAAGTTAGGATCATACAACCCTTCAACTTCCAGAAAGCTTCATGGCAACACTTTGTCCTGCAAGGCAGCTTCTTCTATCACAGATTTTGATCTTTATGATCTACTTGGCATTGACAACACCTCCCATCCATCACGGATTAAGGCGGCCTACCGTGCACTCCAAAAACACTGCCACCCCGACATCGCCGGTCCTGCTGGCCACGACATGGCCATCATTCTTAATGAAGCATACTCTGTTCTATCAGATCCTAGTTCTAGGCTGGCTTATGATAAGGTCAGAATAGGTAGCACACTATTCTAATTGTAATTGGTTTCAGCAAACTATGGTTTTTGACGTGAGAATTATGTCCCACAGGAGCAAGCAAAAATGGCAGAACTTCGAGGTTACACAGGGAAGCCAGTTTATTCAGTGTGGTTCGGGTCAGAAAGCGAACAACGGGCGGTTTTTGTGGATGAAGTGAAATGTATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTTGAATCTGTGTATGGGAGAGCTAGAGTTGTGGCTCAATGGGCTGATCCTGAATATAAAGTTATGGAGGCTATCGAAGCTTGCCCAGTTGACTGCATTTCGTGAGTTGTGTTGTTATATTTTGATTTCAAAATTCCTGTCTCTCTCTCCTGATTAGTTTATAAGTTTCTTTTTTTTCTTGGTAAATTCAACACAGCATGGTGGAAAGGACAGACTTGGCAGCCTTGGAGTTCTTAATGTCGAAGCAACCACGTGGGAACGTAAGAGTTGGTATGGGGAACACAGCTGGCGAACGAGTGTCAAACATCTTCACTGATGTGAAGAAGTTCCAAATCAAATTCAATGAGGCTATGGAGAAAGCTATAAAAGAGCAATCCAAGGTTATACTTAGACATAGTAATGGCAACAGTTCAATAATTGATGGTACCTTCTTTAATGGCTTACTGAGATCATCGAGTTGTATGATTGGTTTTACAGGGAGCAACCTTTGAGAGCGAAGGACAGTTGGCTGCAATTCAGGCTATCAGATCAATCTCAAATTGGCTATTTTGGCAGACAGCAACACCAGTTGGACCTGGATCGAAACAAAGCCAAAGCCTGGCACGCAGTGCCAACAAATTCACACCCGAAATCAACAAACTTCAGGCTGCCGCTACTGCAAGGAAACAAATCAGGGAAAAAGCTGAAGACAGAAATAGAACTACAACGAAATACCTATACAGGGATGATTATTGGGTTCCAACCACCTTTGCACTTCCAGCCTCAACTCACTCTCCAAACATCCCAATCTCAAAGCCAAGTGTAGAGACCAAACCTACAAAGCAATCAAGAGACCTTGGTTCTGATGTTAATAGTGGTGGTCACGTTAGTCCCATGAGATTGGTATTACCAGTTTCGATATCGATAATTGCAACTGCTATAATTCAGCAAATGGTGAGAAATGATGGTGCCAGTGAATTGAAGGAACACGCTGCAGGTTCTATGGCTCTGGAGTTAGTTAATAGTCACTGGATGCAGGTGATCCTAACAGGCGTCACTTGGTATATCATTGGAATGGCATTGATGGGAATGGTAGAAATGATTGCAAGAAAGTTTAGGCCTTGA

mRNA sequence

ATGAGTGCCTCATGGCTTCCTCTGTACACACCCACAACACAATATCCTATTCAAAGAAAGTTAGGATCATACAACCCTTCAACTTCCAGAAAGCTTCATGGCAACACTTTGTCCTGCAAGGCAGCTTCTTCTATCACAGATTTTGATCTTTATGATCTACTTGGCATTGACAACACCTCCCATCCATCACGGATTAAGGCGGCCTACCGTGCACTCCAAAAACACTGCCACCCCGACATCGCCGGTCCTGCTGGCCACGACATGGCCATCATTCTTAATGAAGCATACTCTGTTCTATCAGATCCTAGTTCTAGGCTGGCTTATGATAAGGAGCAAGCAAAAATGGCAGAACTTCGAGGTTACACAGGGAAGCCAGTTTATTCAGTGTGGTTCGGGTCAGAAAGCGAACAACGGGCGGTTTTTGTGGATGAAGTGAAATGTATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTTGAATCTGTGTATGGGAGAGCTAGAGTTGTGGCTCAATGGGCTGATCCTGAATATAAAGTTATGGAGGCTATCGAAGCTTGCCCAGTTGACTGCATTTCCATGGTGGAAAGGACAGACTTGGCAGCCTTGGAGTTCTTAATGTCGAAGCAACCACGTGGGAACGTAAGAGTTGGTATGGGGAACACAGCTGGCGAACGAGTGTCAAACATCTTCACTGATGTGAAGAAGTTCCAAATCAAATTCAATGAGGCTATGGAGAAAGCTATAAAAGAGCAATCCAAGGGAGCAACCTTTGAGAGCGAAGGACAGTTGGCTGCAATTCAGGCTATCAGATCAATCTCAAATTGGCTATTTTGGCAGACAGCAACACCAGTTGGACCTGGATCGAAACAAAGCCAAAGCCTGGCACGCAGTGCCAACAAATTCACACCCGAAATCAACAAACTTCAGGCTGCCGCTACTGCAAGGAAACAAATCAGGGAAAAAGCTGAAGACAGAAATAGAACTACAACGAAATACCTATACAGGGATGATTATTGGGTTCCAACCACCTTTGCACTTCCAGCCTCAACTCACTCTCCAAACATCCCAATCTCAAAGCCAAGTGTAGAGACCAAACCTACAAAGCAATCAAGAGACCTTGGTTCTGATGTTAATAGTGGTGGTCACGTTAGTCCCATGAGATTGGTATTACCAGTTTCGATATCGATAATTGCAACTGCTATAATTCAGCAAATGGTGAGAAATGATGGTGCCAGTGAATTGAAGGAACACGCTGCAGGTTCTATGGCTCTGGAGTTAGTTAATAGTCACTGGATGCAGGTGATCCTAACAGGCGTCACTTGGTATATCATTGGAATGGCATTGATGGGAATGGTAGAAATGATTGCAAGAAAGTTTAGGCCTTGA

Coding sequence (CDS)

ATGAGTGCCTCATGGCTTCCTCTGTACACACCCACAACACAATATCCTATTCAAAGAAAGTTAGGATCATACAACCCTTCAACTTCCAGAAAGCTTCATGGCAACACTTTGTCCTGCAAGGCAGCTTCTTCTATCACAGATTTTGATCTTTATGATCTACTTGGCATTGACAACACCTCCCATCCATCACGGATTAAGGCGGCCTACCGTGCACTCCAAAAACACTGCCACCCCGACATCGCCGGTCCTGCTGGCCACGACATGGCCATCATTCTTAATGAAGCATACTCTGTTCTATCAGATCCTAGTTCTAGGCTGGCTTATGATAAGGAGCAAGCAAAAATGGCAGAACTTCGAGGTTACACAGGGAAGCCAGTTTATTCAGTGTGGTTCGGGTCAGAAAGCGAACAACGGGCGGTTTTTGTGGATGAAGTGAAATGTATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTTGAATCTGTGTATGGGAGAGCTAGAGTTGTGGCTCAATGGGCTGATCCTGAATATAAAGTTATGGAGGCTATCGAAGCTTGCCCAGTTGACTGCATTTCCATGGTGGAAAGGACAGACTTGGCAGCCTTGGAGTTCTTAATGTCGAAGCAACCACGTGGGAACGTAAGAGTTGGTATGGGGAACACAGCTGGCGAACGAGTGTCAAACATCTTCACTGATGTGAAGAAGTTCCAAATCAAATTCAATGAGGCTATGGAGAAAGCTATAAAAGAGCAATCCAAGGGAGCAACCTTTGAGAGCGAAGGACAGTTGGCTGCAATTCAGGCTATCAGATCAATCTCAAATTGGCTATTTTGGCAGACAGCAACACCAGTTGGACCTGGATCGAAACAAAGCCAAAGCCTGGCACGCAGTGCCAACAAATTCACACCCGAAATCAACAAACTTCAGGCTGCCGCTACTGCAAGGAAACAAATCAGGGAAAAAGCTGAAGACAGAAATAGAACTACAACGAAATACCTATACAGGGATGATTATTGGGTTCCAACCACCTTTGCACTTCCAGCCTCAACTCACTCTCCAAACATCCCAATCTCAAAGCCAAGTGTAGAGACCAAACCTACAAAGCAATCAAGAGACCTTGGTTCTGATGTTAATAGTGGTGGTCACGTTAGTCCCATGAGATTGGTATTACCAGTTTCGATATCGATAATTGCAACTGCTATAATTCAGCAAATGGTGAGAAATGATGGTGCCAGTGAATTGAAGGAACACGCTGCAGGTTCTATGGCTCTGGAGTTAGTTAATAGTCACTGGATGCAGGTGATCCTAACAGGCGTCACTTGGTATATCATTGGAATGGCATTGATGGGAATGGTAGAAATGATTGCAAGAAAGTTTAGGCCTTGA

Protein sequence

MSASWLPLYTPTTQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRGYTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKFQIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLARSANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSPNIPISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFRP*
Homology
BLAST of Chy10G178390 vs. ExPASy Swiss-Prot
Match: Q9FMX6 (Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC76 PE=2 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.5e-128
Identity = 248/465 (53.33%), Postives = 315/465 (67.74%), Query Frame = 0

Query: 8   LYTPTTQYPIQRKLGSYNPSTSRKL----HGNTLSCKA---ASSITDFDLYDLLGIDNTS 67
           +++PTT  P      ++  STS+KL          C+A   +SSITDFDLYDLLGID +S
Sbjct: 5   IFSPTTLPP---STATWPCSTSQKLITVRSPLKFKCRATSSSSSITDFDLYDLLGIDRSS 64

Query: 68  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 127
             S+IK+AYRALQK CHPDIAG  GHDMAIILNEAY +LSDP SR AYDKEQAK+ ELRG
Sbjct: 65  DKSQIKSAYRALQKRCHPDIAGDPGHDMAIILNEAYQLLSDPISRQAYDKEQAKLEELRG 124

Query: 128 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 187
           YTGKP+YSVW G E+EQRA FVDEVKC+GCLKCAL A KTFA+E+ YGRARVVAQWADPE
Sbjct: 125 YTGKPIYSVWCGPETEQRAAFVDEVKCVGCLKCALCAEKTFAIETAYGRARVVAQWADPE 184

Query: 188 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 247
            K+ EAIEACPVDCISMVER+DLA LEFLMSKQPRGNVR+G+GNT GERVSN+F DVKKF
Sbjct: 185 SKIKEAIEACPVDCISMVERSDLAPLEFLMSKQPRGNVRIGVGNTVGERVSNVFVDVKKF 244

Query: 248 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTA---TPVGPGSKQSQS 307
           Q ++ +AM +  KE     T + E Q++A++AIRSISNWL+W+++    P+ P S  S +
Sbjct: 245 QERYAKAMSRTTKE-----TSQREVQISAVEAIRSISNWLYWRSSPYTKPLSPESNMSLT 304

Query: 308 LARSANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSP 367
             +      P+I KLQ    A KQ  +    + + +  YL  +DYW P+  ALP+S ++ 
Sbjct: 305 FTKRKKAVDPDIRKLQDVVAAMKQADQSGRTKEKGSA-YLLGEDYWSPSNAALPSSGNNN 364

Query: 368 NIPISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASE 427
               S     T+ T  S +  +          ++   P+  +I+A  ++Q       ASE
Sbjct: 365 GSKASSNPQVTRKTFPSEEKPTSRRENRRQFRIK-KFPIGTAIVAVFLVQYQASYRAASE 424

Query: 428 LKEHAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARK 463
           L +H  GS+AL +VNS W Q++L GVTWY IG  L+ +VE +  K
Sbjct: 425 LNDHIGGSLALSIVNSPWQQILLAGVTWYFIGAMLLQLVEAVQHK 459

BLAST of Chy10G178390 vs. ExPASy Swiss-Prot
Match: Q8G6C6 (Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.1e-08
Identity = 36/94 (38.30%), Postives = 47/94 (50.00%), Query Frame = 0

Query: 49  DLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAY 108
           D Y+ LG++  +    IK AYR L +  HPDIAGP   D    +N AY VLS+P  R  Y
Sbjct: 3   DYYETLGVERGASDDEIKKAYRKLSRKYHPDIAGPEFEDKFKEVNNAYDVLSNPDKRRMY 62

Query: 109 DK---EQAKMAELRGYTG------KPVYSVWFGS 134
           D         A   G++G        V+S +FGS
Sbjct: 63  DSGVDPNNPNAGAGGFSGAGFGDMSDVFSTFFGS 96

BLAST of Chy10G178390 vs. ExPASy Swiss-Prot
Match: Q98PI9 (Chaperone protein DnaJ OS=Mycoplasma pulmonis (strain UAB CTIP) OX=272635 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 3.0e-07
Identity = 32/86 (37.21%), Postives = 45/86 (52.33%), Query Frame = 0

Query: 49  DLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAI-ILNEAYSVLSDPSSRLA 108
           D Y +LGID +++   IK AYR L    HPD +     +  +  +NEAY VLS+P  +  
Sbjct: 6   DYYKILGIDKSANEKEIKKAYRKLAMEHHPDRSSSKESEAKMREINEAYEVLSNPEKKAI 65

Query: 109 YDKEQAKMAELRGYTGKPVYSVWFGS 134
           YDK   + A   G+ G    S  FG+
Sbjct: 66  YDKYGHEAANNPGFNGFNAGSAGFGN 91

BLAST of Chy10G178390 vs. ExPASy Swiss-Prot
Match: Q6A997 (Chaperone protein DnaJ 1 OS=Cutibacterium acnes (strain DSM 16379 / KPA171202) OX=267747 GN=dnaJ1 PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.9e-07
Identity = 25/73 (34.25%), Postives = 38/73 (52.05%), Query Frame = 0

Query: 49  DLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAY 108
           D Y++LG+ + +    IK AYR      HPD+AGP   +    + EAY +L DP  R  +
Sbjct: 4   DYYEILGVSHDASADEIKKAYRRKAMKLHPDVAGPGSEEEFKKVQEAYEILQDPQKRAVF 63

Query: 109 DKEQAKMAELRGY 122
           D+     A + G+
Sbjct: 64  DRGGDPNARMGGF 76

BLAST of Chy10G178390 vs. ExPASy Swiss-Prot
Match: Q9PQ82 (Chaperone protein DnaJ OS=Ureaplasma parvum serovar 3 (strain ATCC 700970) OX=273119 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 8.6e-07
Identity = 27/62 (43.55%), Postives = 36/62 (58.06%), Query Frame = 0

Query: 49  DLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAY 108
           D Y++LG+  ++ P  IKAA+R L K  HPD    A   +   +NEAY VLSD   R  Y
Sbjct: 5   DYYEILGVSKSATPEEIKAAFRKLAKEHHPDRNKSADDTLFKEINEAYEVLSDSKKRAQY 64

Query: 109 DK 111
           D+
Sbjct: 65  DQ 66

BLAST of Chy10G178390 vs. ExPASy TrEMBL
Match: A0A0A0KPY4 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175710 PE=4 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.1e-258
Identity = 454/465 (97.63%), Postives = 459/465 (98.71%), Query Frame = 0

Query: 1   MSASWLPLYTPTTQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60
           MSASWLPLYTPT QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS
Sbjct: 1   MSASWLPLYTPTAQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60

Query: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120
           HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG
Sbjct: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120

Query: 121 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180
           YTGKPVYSVW GSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE
Sbjct: 121 YTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180

Query: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240
           YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF
Sbjct: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240

Query: 241 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300
           QIKFNEAMEKA+KEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR
Sbjct: 241 QIKFNEAMEKAMKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300

Query: 301 SANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSPNIP 360
           SA+KFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPAST SPN P
Sbjct: 301 SASKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTQSPNNP 360

Query: 361 ISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420
           ISKPSVETKPTKQSR LGSDV+ GGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE
Sbjct: 361 ISKPSVETKPTKQSRGLGSDVSRGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420

Query: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFRP 466
           HAAGSMALELVNSHWMQVILTGVTWYIIGMA+MGM+EMIARKFRP
Sbjct: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMAVMGMLEMIARKFRP 465

BLAST of Chy10G178390 vs. ExPASy TrEMBL
Match: A0A5A7SY19 (Putative DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00570 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.2e-250
Identity = 443/465 (95.27%), Postives = 450/465 (96.77%), Query Frame = 0

Query: 1   MSASWLPLYTPTTQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60
           MSASWLPLYTPT QYPIQRKLGSYNPSTSRKLH NTLSCKAASSITDFDLYDLLGIDNTS
Sbjct: 1   MSASWLPLYTPTKQYPIQRKLGSYNPSTSRKLHANTLSCKAASSITDFDLYDLLGIDNTS 60

Query: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120
           +PSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG
Sbjct: 61  NPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120

Query: 121 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180
           YTGKPVYSVW GSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE
Sbjct: 121 YTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180

Query: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240
           YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF
Sbjct: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240

Query: 241 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300
           QI+FNEAMEKA KEQSKGATF+SEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSL R
Sbjct: 241 QIRFNEAMEKATKEQSKGATFDSEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLTR 300

Query: 301 SANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSPNIP 360
           SA+KFTPEINKLQAA TARKQIREKAE RNR TTKYL RDDYWVPTTFALPAST  PN P
Sbjct: 301 SASKFTPEINKLQAAVTARKQIREKAEGRNRITTKYLSRDDYWVPTTFALPASTQCPNNP 360

Query: 361 ISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420
           ISKPSVETKPTKQSRDLGSDV+SG HVSPMRL+LPVSISIIATAIIQQMVRNDGA ELKE
Sbjct: 361 ISKPSVETKPTKQSRDLGSDVSSGVHVSPMRLILPVSISIIATAIIQQMVRNDGADELKE 420

Query: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFRP 466
           HAAGSMALELVNSHWMQVILTGVTWYIIGMA+ GMVEMIARKFRP
Sbjct: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMAVTGMVEMIARKFRP 465

BLAST of Chy10G178390 vs. ExPASy TrEMBL
Match: A0A1S3BDQ8 (uncharacterized protein LOC103488766 OS=Cucumis melo OX=3656 GN=LOC103488766 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.2e-250
Identity = 443/465 (95.27%), Postives = 450/465 (96.77%), Query Frame = 0

Query: 1   MSASWLPLYTPTTQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60
           MSASWLPLYTPT QYPIQRKLGSYNPSTSRKLH NTLSCKAASSITDFDLYDLLGIDNTS
Sbjct: 1   MSASWLPLYTPTKQYPIQRKLGSYNPSTSRKLHANTLSCKAASSITDFDLYDLLGIDNTS 60

Query: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120
           +PSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG
Sbjct: 61  NPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120

Query: 121 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180
           YTGKPVYSVW GSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE
Sbjct: 121 YTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180

Query: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240
           YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF
Sbjct: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240

Query: 241 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300
           QI+FNEAMEKA KEQSKGATF+SEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSL R
Sbjct: 241 QIRFNEAMEKATKEQSKGATFDSEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLTR 300

Query: 301 SANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSPNIP 360
           SA+KFTPEINKLQAA TARKQIREKAE RNR TTKYL RDDYWVPTTFALPAST  PN P
Sbjct: 301 SASKFTPEINKLQAAVTARKQIREKAEGRNRITTKYLSRDDYWVPTTFALPASTQCPNNP 360

Query: 361 ISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420
           ISKPSVETKPTKQSRDLGSDV+SG HVSPMRL+LPVSISIIATAIIQQMVRNDGA ELKE
Sbjct: 361 ISKPSVETKPTKQSRDLGSDVSSGVHVSPMRLILPVSISIIATAIIQQMVRNDGADELKE 420

Query: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFRP 466
           HAAGSMALELVNSHWMQVILTGVTWYIIGMA+ GMVEMIARKFRP
Sbjct: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMAVTGMVEMIARKFRP 465

BLAST of Chy10G178390 vs. ExPASy TrEMBL
Match: A0A6J1GZ80 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458571 PE=4 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.0e-215
Identity = 387/469 (82.52%), Postives = 409/469 (87.21%), Query Frame = 0

Query: 1   MSASWLPLYTPTT-----QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLG 60
           MS SWLPLYT T      QYP Q KLGSYN STSRK +GNTLSC+AASSITDFDLYDLLG
Sbjct: 1   MSTSWLPLYTHTAAATARQYPTQTKLGSYNSSTSRKPYGNTLSCRAASSITDFDLYDLLG 60

Query: 61  IDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKM 120
           ID++S  SRIKAAYRALQK CHPDIAGPAGHDM+IILNEAYS+LSDPSSRLAYDKEQAKM
Sbjct: 61  IDSSSDSSRIKAAYRALQKQCHPDIAGPAGHDMSIILNEAYSLLSDPSSRLAYDKEQAKM 120

Query: 121 AELRGYTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180
           AELRGYTG PVYSVW GSE+EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ
Sbjct: 121 AELRGYTGNPVYSVWLGSENEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180

Query: 181 WADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFT 240
           WADPEYKVMEAI+ CPVDCISMVER+DLAALEFLMSKQPRGNVRVGM N AGERVSNIFT
Sbjct: 181 WADPEYKVMEAIQTCPVDCISMVERSDLAALEFLMSKQPRGNVRVGMSNAAGERVSNIFT 240

Query: 241 DVKKFQIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQS 300
           DVKKFQ +F EAMEKA KE SKGATFE+E QLAAIQ IRSISNWLFWQTATP GPGSKQ 
Sbjct: 241 DVKKFQARFKEAMEKASKEHSKGATFENEAQLAAIQTIRSISNWLFWQTATPAGPGSKQG 300

Query: 301 QSLARSANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTH 360
           Q+L   A K TPEIN+LQAA TARKQIR KAEDRNR T K++Y+DDYWVPTT ALP ST 
Sbjct: 301 QNLTHIATKSTPEINRLQAAVTARKQIRAKAEDRNRATEKHIYKDDYWVPTTLALPTSTQ 360

Query: 361 SPNIPISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGA 420
           +PN  ISK  VETKP K+ R LG     GGHVSPMRL LP+SISIIATA IQQM+RNDGA
Sbjct: 361 TPNSTISKSRVETKPIKEPRGLG----DGGHVSPMRLALPISISIIATAAIQQMMRNDGA 420

Query: 421 SELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFR 465
           S LKEH  GSMALELVNSHWMQV+LTGVTWYIIGM +MGMVEMI RK R
Sbjct: 421 SSLKEHIGGSMALELVNSHWMQVMLTGVTWYIIGMVVMGMVEMIGRKIR 465

BLAST of Chy10G178390 vs. ExPASy TrEMBL
Match: A0A6J1KG86 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493617 PE=4 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.5e-213
Identity = 384/469 (81.88%), Postives = 409/469 (87.21%), Query Frame = 0

Query: 1   MSASWLPLYTPTT-----QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLG 60
           MS SWLPLYT T      QYP + KL SYN STSRKL+GNTLSC+AASSI DFDLYDLLG
Sbjct: 1   MSTSWLPLYTHTAAATARQYPTRTKLASYN-STSRKLYGNTLSCRAASSIRDFDLYDLLG 60

Query: 61  IDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKM 120
           ID++S  SRIKAAYRALQK CHPDIAGPAGHDM+IILNEAYS+LSDPSSRLAYDKEQAK+
Sbjct: 61  IDSSSDSSRIKAAYRALQKQCHPDIAGPAGHDMSIILNEAYSLLSDPSSRLAYDKEQAKV 120

Query: 121 AELRGYTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180
           AELRGYTG PVYSVW GS++EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ
Sbjct: 121 AELRGYTGNPVYSVWLGSQNEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180

Query: 181 WADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFT 240
           WADPEYKVMEAI+ CPVDCIS+VER+DLAALEFLMSKQPRGNVRVGM N AGERVSNIFT
Sbjct: 181 WADPEYKVMEAIQTCPVDCISIVERSDLAALEFLMSKQPRGNVRVGMSNAAGERVSNIFT 240

Query: 241 DVKKFQIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQS 300
           DVKKFQ +F EAMEKA KE SKGATFE+E QLAAIQ IRSISNWLFWQTATP GPGSKQ 
Sbjct: 241 DVKKFQARFKEAMEKASKEHSKGATFENEAQLAAIQTIRSISNWLFWQTATPAGPGSKQG 300

Query: 301 QSLARSANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTH 360
           Q+L   A K TPEIN+LQAA TARKQIR KAEDRNR T K++Y+DDYWVPTT ALP ST 
Sbjct: 301 QNLTHIATKCTPEINRLQAAVTARKQIRAKAEDRNRATEKHIYKDDYWVPTTLALPTSTQ 360

Query: 361 SPNIPISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGA 420
           +PN  ISK  VETKPTK+ R LG     GGHVSPMRL LPVSISIIATA IQQM+RNDGA
Sbjct: 361 TPNSTISKSRVETKPTKEPRGLG----DGGHVSPMRLALPVSISIIATAAIQQMMRNDGA 420

Query: 421 SELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFR 465
           S LKEH  GSMALELVNSHWMQV+LTGVTWYIIGM +MGMVEMI RK R
Sbjct: 421 SSLKEHVGGSMALELVNSHWMQVMLTGVTWYIIGMVVMGMVEMIGRKIR 464

BLAST of Chy10G178390 vs. NCBI nr
Match: XP_011654903.1 (chaperone protein dnaJ C76, chloroplastic [Cucumis sativus] >KGN50447.1 hypothetical protein Csa_000192 [Cucumis sativus])

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 454/465 (97.63%), Postives = 459/465 (98.71%), Query Frame = 0

Query: 1   MSASWLPLYTPTTQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60
           MSASWLPLYTPT QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS
Sbjct: 1   MSASWLPLYTPTAQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60

Query: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120
           HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG
Sbjct: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120

Query: 121 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180
           YTGKPVYSVW GSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE
Sbjct: 121 YTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180

Query: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240
           YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF
Sbjct: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240

Query: 241 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300
           QIKFNEAMEKA+KEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR
Sbjct: 241 QIKFNEAMEKAMKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300

Query: 301 SANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSPNIP 360
           SA+KFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPAST SPN P
Sbjct: 301 SASKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTQSPNNP 360

Query: 361 ISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420
           ISKPSVETKPTKQSR LGSDV+ GGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE
Sbjct: 361 ISKPSVETKPTKQSRGLGSDVSRGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420

Query: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFRP 465
           HAAGSMALELVNSHWMQVILTGVTWYIIGMA+MGM+EMIARKFRP
Sbjct: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMAVMGMLEMIARKFRP 465

BLAST of Chy10G178390 vs. NCBI nr
Match: XP_008445880.1 (PREDICTED: uncharacterized protein LOC103488766 [Cucumis melo] >KAA0034069.1 putative DNAJ heat shock N-terminal domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 874 bits (2257), Expect = 0.0
Identity = 443/465 (95.27%), Postives = 450/465 (96.77%), Query Frame = 0

Query: 1   MSASWLPLYTPTTQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60
           MSASWLPLYTPT QYPIQRKLGSYNPSTSRKLH NTLSCKAASSITDFDLYDLLGIDNTS
Sbjct: 1   MSASWLPLYTPTKQYPIQRKLGSYNPSTSRKLHANTLSCKAASSITDFDLYDLLGIDNTS 60

Query: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120
           +PSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG
Sbjct: 61  NPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120

Query: 121 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180
           YTGKPVYSVW GSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE
Sbjct: 121 YTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180

Query: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240
           YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF
Sbjct: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240

Query: 241 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300
           QI+FNEAMEKA KEQSKGATF+SEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSL R
Sbjct: 241 QIRFNEAMEKATKEQSKGATFDSEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLTR 300

Query: 301 SANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSPNIP 360
           SA+KFTPEINKLQAA TARKQIREKAE RNR TTKYL RDDYWVPTTFALPAST  PN P
Sbjct: 301 SASKFTPEINKLQAAVTARKQIREKAEGRNRITTKYLSRDDYWVPTTFALPASTQCPNNP 360

Query: 361 ISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420
           ISKPSVETKPTKQSRDLGSDV+SG HVSPMRL+LPVSISIIATAIIQQMVRNDGA ELKE
Sbjct: 361 ISKPSVETKPTKQSRDLGSDVSSGVHVSPMRLILPVSISIIATAIIQQMVRNDGADELKE 420

Query: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFRP 465
           HAAGSMALELVNSHWMQVILTGVTWYIIGMA+ GMVEMIARKFRP
Sbjct: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMAVTGMVEMIARKFRP 465

BLAST of Chy10G178390 vs. NCBI nr
Match: XP_038892355.1 (chaperone protein dnaJ C76, chloroplastic [Benincasa hispida])

HSP 1 Score: 829 bits (2141), Expect = 1.26e-300
Identity = 418/465 (89.89%), Postives = 435/465 (93.55%), Query Frame = 0

Query: 1   MSASWLPLYTPTTQYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGIDNTS 60
           MSASWLPLYTPT QYPIQR+L  Y PSTSRKLHGNTLSC+AASSITDFDLYDLLGIDNT+
Sbjct: 44  MSASWLPLYTPTRQYPIQRRLEPYIPSTSRKLHGNTLSCRAASSITDFDLYDLLGIDNTA 103

Query: 61  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 120
           +PSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDP SRLAYDKEQAKMAELRG
Sbjct: 104 NPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPCSRLAYDKEQAKMAELRG 163

Query: 121 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 180
           YTGKPVYSVW GSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE
Sbjct: 164 YTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 223

Query: 181 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 240
           YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGN AGERVSNIFTDVKKF
Sbjct: 224 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTDVKKF 283

Query: 241 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQSLAR 300
           QI+FNEAMEKA KEQSKGATFESE QLAAIQAIRSISNWLFWQTATP GPGSK  Q+LAR
Sbjct: 284 QIRFNEAMEKATKEQSKGATFESEAQLAAIQAIRSISNWLFWQTATPAGPGSKHGQNLAR 343

Query: 301 SANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSPNIP 360
           SA+KFTPEIN+LQAA TARKQ+REKAEDRNRTT KYLY+DDYWVPTTFALPAST S N  
Sbjct: 344 SASKFTPEINRLQAAVTARKQVREKAEDRNRTTAKYLYKDDYWVPTTFALPASTQSQNNT 403

Query: 361 ISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASELKE 420
           ISKP VETKPTK+S +LG DV  GGHVS MRL LPVSISI+AT IIQQMV NDGAS LKE
Sbjct: 404 ISKPRVETKPTKESGELGFDVGDGGHVSLMRLALPVSISIVATTIIQQMVENDGASGLKE 463

Query: 421 HAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFRP 465
           HAAGSMAL+LVNS WMQV+LTGVTWYIIGMA++GMV MIARKFRP
Sbjct: 464 HAAGSMALDLVNSRWMQVMLTGVTWYIIGMAVLGMVGMIARKFRP 508

BLAST of Chy10G178390 vs. NCBI nr
Match: XP_023543201.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 758 bits (1957), Expect = 2.75e-273
Identity = 387/469 (82.52%), Postives = 409/469 (87.21%), Query Frame = 0

Query: 1   MSASWLPLYTPTT-----QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLG 60
           MS SWLPLYT T      QYP Q KLGSYN STSRK +GNTLSC+AASSITDFDLYDLLG
Sbjct: 1   MSTSWLPLYTHTAAATARQYPTQTKLGSYNSSTSRKPYGNTLSCRAASSITDFDLYDLLG 60

Query: 61  IDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKM 120
           ID++S  SRIKAAYRALQK CHPDIAGPAGHDM+IILNEAYS+LSDPSSRLAYDKEQAKM
Sbjct: 61  IDSSSDSSRIKAAYRALQKQCHPDIAGPAGHDMSIILNEAYSLLSDPSSRLAYDKEQAKM 120

Query: 121 AELRGYTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180
           AELRGYTG PVYSVW GSE+EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ
Sbjct: 121 AELRGYTGNPVYSVWLGSENEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180

Query: 181 WADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFT 240
           WADPEYKVMEAI+ CPVDCISMVER+DLAALEFLMSKQPRGNVRVGM N AGERVSNIFT
Sbjct: 181 WADPEYKVMEAIQTCPVDCISMVERSDLAALEFLMSKQPRGNVRVGMSNAAGERVSNIFT 240

Query: 241 DVKKFQIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQS 300
           DVKKFQ +F EAMEKA KE SKG TFE+E QLAAIQ IRSISNWLFWQTATP GPGSKQ 
Sbjct: 241 DVKKFQARFKEAMEKASKEHSKGPTFENEAQLAAIQTIRSISNWLFWQTATPAGPGSKQG 300

Query: 301 QSLARSANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTH 360
           Q+L   A K TPEIN+LQAA TARKQIR KAEDRNR T KY+++DDYWVPTT ALP ST 
Sbjct: 301 QNLTHIATKSTPEINRLQAAVTARKQIRAKAEDRNRATAKYIHKDDYWVPTTLALPTSTQ 360

Query: 361 SPNIPISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGA 420
           +PN  ISK  VETKPTK+ R LG     GGHVSPMRL +PVSISIIATA IQQM+RNDGA
Sbjct: 361 TPNSTISKSRVETKPTKEPRGLGD----GGHVSPMRLAIPVSISIIATAAIQQMMRNDGA 420

Query: 421 SELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFR 464
           S LKEH  GSMALELVNSHWMQV+LTGVTWYIIGM +MGMVEMI RK R
Sbjct: 421 SSLKEHIGGSMALELVNSHWMQVMLTGVTWYIIGMVVMGMVEMIGRKIR 465

BLAST of Chy10G178390 vs. NCBI nr
Match: XP_022957083.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 758 bits (1956), Expect = 3.90e-273
Identity = 387/469 (82.52%), Postives = 409/469 (87.21%), Query Frame = 0

Query: 1   MSASWLPLYTPTT-----QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLG 60
           MS SWLPLYT T      QYP Q KLGSYN STSRK +GNTLSC+AASSITDFDLYDLLG
Sbjct: 1   MSTSWLPLYTHTAAATARQYPTQTKLGSYNSSTSRKPYGNTLSCRAASSITDFDLYDLLG 60

Query: 61  IDNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKM 120
           ID++S  SRIKAAYRALQK CHPDIAGPAGHDM+IILNEAYS+LSDPSSRLAYDKEQAKM
Sbjct: 61  IDSSSDSSRIKAAYRALQKQCHPDIAGPAGHDMSIILNEAYSLLSDPSSRLAYDKEQAKM 120

Query: 121 AELRGYTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180
           AELRGYTG PVYSVW GSE+EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ
Sbjct: 121 AELRGYTGNPVYSVWLGSENEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180

Query: 181 WADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFT 240
           WADPEYKVMEAI+ CPVDCISMVER+DLAALEFLMSKQPRGNVRVGM N AGERVSNIFT
Sbjct: 181 WADPEYKVMEAIQTCPVDCISMVERSDLAALEFLMSKQPRGNVRVGMSNAAGERVSNIFT 240

Query: 241 DVKKFQIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQS 300
           DVKKFQ +F EAMEKA KE SKGATFE+E QLAAIQ IRSISNWLFWQTATP GPGSKQ 
Sbjct: 241 DVKKFQARFKEAMEKASKEHSKGATFENEAQLAAIQTIRSISNWLFWQTATPAGPGSKQG 300

Query: 301 QSLARSANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTH 360
           Q+L   A K TPEIN+LQAA TARKQIR KAEDRNR T K++Y+DDYWVPTT ALP ST 
Sbjct: 301 QNLTHIATKSTPEINRLQAAVTARKQIRAKAEDRNRATEKHIYKDDYWVPTTLALPTSTQ 360

Query: 361 SPNIPISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGA 420
           +PN  ISK  VETKP K+ R LG     GGHVSPMRL LP+SISIIATA IQQM+RNDGA
Sbjct: 361 TPNSTISKSRVETKPIKEPRGLGD----GGHVSPMRLALPISISIIATAAIQQMMRNDGA 420

Query: 421 SELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARKFR 464
           S LKEH  GSMALELVNSHWMQV+LTGVTWYIIGM +MGMVEMI RK R
Sbjct: 421 SSLKEHIGGSMALELVNSHWMQVMLTGVTWYIIGMVVMGMVEMIGRKIR 465

BLAST of Chy10G178390 vs. TAIR 10
Match: AT5G23240.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 461.1 bits (1185), Expect = 1.1e-129
Identity = 248/465 (53.33%), Postives = 315/465 (67.74%), Query Frame = 0

Query: 8   LYTPTTQYPIQRKLGSYNPSTSRKL----HGNTLSCKA---ASSITDFDLYDLLGIDNTS 67
           +++PTT  P      ++  STS+KL          C+A   +SSITDFDLYDLLGID +S
Sbjct: 5   IFSPTTLPP---STATWPCSTSQKLITVRSPLKFKCRATSSSSSITDFDLYDLLGIDRSS 64

Query: 68  HPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMAELRG 127
             S+IK+AYRALQK CHPDIAG  GHDMAIILNEAY +LSDP SR AYDKEQAK+ ELRG
Sbjct: 65  DKSQIKSAYRALQKRCHPDIAGDPGHDMAIILNEAYQLLSDPISRQAYDKEQAKLEELRG 124

Query: 128 YTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPE 187
           YTGKP+YSVW G E+EQRA FVDEVKC+GCLKCAL A KTFA+E+ YGRARVVAQWADPE
Sbjct: 125 YTGKPIYSVWCGPETEQRAAFVDEVKCVGCLKCALCAEKTFAIETAYGRARVVAQWADPE 184

Query: 188 YKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTDVKKF 247
            K+ EAIEACPVDCISMVER+DLA LEFLMSKQPRGNVR+G+GNT GERVSN+F DVKKF
Sbjct: 185 SKIKEAIEACPVDCISMVERSDLAPLEFLMSKQPRGNVRIGVGNTVGERVSNVFVDVKKF 244

Query: 248 QIKFNEAMEKAIKEQSKGATFESEGQLAAIQAIRSISNWLFWQTA---TPVGPGSKQSQS 307
           Q ++ +AM +  KE     T + E Q++A++AIRSISNWL+W+++    P+ P S  S +
Sbjct: 245 QERYAKAMSRTTKE-----TSQREVQISAVEAIRSISNWLYWRSSPYTKPLSPESNMSLT 304

Query: 308 LARSANKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTHSP 367
             +      P+I KLQ    A KQ  +    + + +  YL  +DYW P+  ALP+S ++ 
Sbjct: 305 FTKRKKAVDPDIRKLQDVVAAMKQADQSGRTKEKGSA-YLLGEDYWSPSNAALPSSGNNN 364

Query: 368 NIPISKPSVETKPTKQSRDLGSDVNSGGHVSPMRLVLPVSISIIATAIIQQMVRNDGASE 427
               S     T+ T  S +  +          ++   P+  +I+A  ++Q       ASE
Sbjct: 365 GSKASSNPQVTRKTFPSEEKPTSRRENRRQFRIK-KFPIGTAIVAVFLVQYQASYRAASE 424

Query: 428 LKEHAAGSMALELVNSHWMQVILTGVTWYIIGMALMGMVEMIARK 463
           L +H  GS+AL +VNS W Q++L GVTWY IG  L+ +VE +  K
Sbjct: 425 LNDHIGGSLALSIVNSPWQQILLAGVTWYFIGAMLLQLVEAVQHK 459

BLAST of Chy10G178390 vs. TAIR 10
Match: AT2G42750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-25
Identity = 75/218 (34.40%), Postives = 110/218 (50.46%), Query Frame = 0

Query: 49  DLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAG--PAGHDMAIILNEAYSVLSDPSSRL 108
           D Y +LG+   +    IK AY    K CHPD++G  P   +  + +N+ Y +LSDP  R+
Sbjct: 76  DYYAVLGLLPDATQEEIKKAYYNCMKSCHPDLSGNDPETTNFCMFINDIYEILSDPVQRM 135

Query: 109 AYDKEQAKMAELRGYTGKPVYSVWFGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESV 168
            YD       E+ GYT   + + +    + +  VFVDE  CIGC  CA  A   F +E  
Sbjct: 136 VYD-------EIHGYTVTAI-NPFLDDSTPRDHVFVDEFACIGCKNCANVAPDIFQIEED 195

Query: 169 YGRARVVAQWADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRV---GMG 228
           +GRAR   Q  +P+  V +A+E CPVDCI       L+ LE  M +  R NV +   GMG
Sbjct: 196 FGRARACNQRGNPDL-VQQAVETCPVDCIHQTSAAQLSLLEDEMRRVERVNVALMLSGMG 255

Query: 229 NTAGERVSNIFTDVKKFQIK-FNEAMEKAIKEQSKGAT 261
           + A +      +  +K Q K  N+A  + +K ++   T
Sbjct: 256 SGAVDVFRMARSRWEKRQAKVLNQARSRMMKRKNTDET 284

BLAST of Chy10G178390 vs. TAIR 10
Match: AT3G05345.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 5.0e-10
Identity = 51/189 (26.98%), Postives = 84/189 (44.44%), Query Frame = 0

Query: 27  STSRKLHGNTLSCKAASSITDFDLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGPAGH 86
           STS++   +         ++    Y +LG++ +   S +KAA+RA  K  HPD+     +
Sbjct: 22  STSQRFIPSCRGKNREDPLSSSSPYSILGVEPSCSSSELKAAFRAKVKQYHPDVNKDGSN 81

Query: 87  DMAII--LNEAYSVLSDPSSRLAYDKEQAKMAELRGYTGKPVYSVWFGSESEQRAVFVDE 146
              +I  + +AY +L++      Y + +    E       P        E E   VFV+E
Sbjct: 82  SDIMIRRIIQAYEMLTN------YSRSEIIEGECLDPFDHP--------ECEALDVFVNE 141

Query: 147 VKCIG---CLKCALFAGKTFAVESVYGRARVVAQWADPEYKVMEAIEACPVDCISMVERT 206
           V C+G      C   A   F+ +S  G AR ++Q    +Y+V  A+  CP +CI  V  +
Sbjct: 142 VLCVGKRCSYPCFETASHVFSCDS-SGTARAMSQGHGEDYRVQSAVNQCPRNCIHYVTPS 195

Query: 207 DLAALEFLM 211
               LE L+
Sbjct: 202 QRIILEELL 195

BLAST of Chy10G178390 vs. TAIR 10
Match: AT1G21080.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 53.9 bits (128), Expect = 4.0e-07
Identity = 32/94 (34.04%), Postives = 46/94 (48.94%), Query Frame = 0

Query: 45  ITDFDLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGP----AGHDMAIILNEAYSVLS 104
           + + + YD+LG+  T+  + IK AY    +  HPD   P    A H+   +L EAY VLS
Sbjct: 2   VKETEFYDVLGVSPTATEAEIKKAYYIKARQVHPD-KNPNDPQAAHNFQ-VLGEAYQVLS 61

Query: 105 DPSSRLAYDKEQAKMAELRGYTGKPVYSVWFGSE 135
           DP  R AYD                ++++ FGSE
Sbjct: 62  DPGQRQAYDTSGKSGISTEIIDPAAIFAMLFGSE 93

BLAST of Chy10G178390 vs. TAIR 10
Match: AT1G21080.3 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 53.9 bits (128), Expect = 4.0e-07
Identity = 32/94 (34.04%), Postives = 46/94 (48.94%), Query Frame = 0

Query: 45  ITDFDLYDLLGIDNTSHPSRIKAAYRALQKHCHPDIAGP----AGHDMAIILNEAYSVLS 104
           + + + YD+LG+  T+  + IK AY    +  HPD   P    A H+   +L EAY VLS
Sbjct: 2   VKETEFYDVLGVSPTATEAEIKKAYYIKARQVHPD-KNPNDPQAAHNFQ-VLGEAYQVLS 61

Query: 105 DPSSRLAYDKEQAKMAELRGYTGKPVYSVWFGSE 135
           DP  R AYD                ++++ FGSE
Sbjct: 62  DPGQRQAYDTSGKSGISTEIIDPAAIFAMLFGSE 93

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMX61.5e-12853.33Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC... [more]
Q8G6C62.1e-0838.30Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=... [more]
Q98PI93.0e-0737.21Chaperone protein DnaJ OS=Mycoplasma pulmonis (strain UAB CTIP) OX=272635 GN=dna... [more]
Q6A9973.9e-0734.25Chaperone protein DnaJ 1 OS=Cutibacterium acnes (strain DSM 16379 / KPA171202) O... [more]
Q9PQ828.6e-0743.55Chaperone protein DnaJ OS=Ureaplasma parvum serovar 3 (strain ATCC 700970) OX=27... [more]
Match NameE-valueIdentityDescription
A0A0A0KPY48.1e-25897.63J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175710 PE=4 SV=1[more]
A0A5A7SY191.2e-25095.27Putative DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo va... [more]
A0A1S3BDQ81.2e-25095.27uncharacterized protein LOC103488766 OS=Cucumis melo OX=3656 GN=LOC103488766 PE=... [more]
A0A6J1GZ801.0e-21582.52chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KG863.5e-21381.88chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_011654903.10.097.63chaperone protein dnaJ C76, chloroplastic [Cucumis sativus] >KGN50447.1 hypothet... [more]
XP_008445880.10.095.27PREDICTED: uncharacterized protein LOC103488766 [Cucumis melo] >KAA0034069.1 put... [more]
XP_038892355.11.26e-30089.89chaperone protein dnaJ C76, chloroplastic [Benincasa hispida][more]
XP_023543201.12.75e-27382.52chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022957083.13.90e-27382.52chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G23240.11.1e-12953.33DNAJ heat shock N-terminal domain-containing protein [more]
AT2G42750.11.1e-2534.40DNAJ heat shock N-terminal domain-containing protein [more]
AT3G05345.15.0e-1026.98Chaperone DnaJ-domain superfamily protein [more]
AT1G21080.14.0e-0734.04DNAJ heat shock N-terminal domain-containing protein [more]
AT1G21080.34.0e-0734.04DNAJ heat shock N-terminal domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 48..104
e-value: 1.1E-13
score: 61.4
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 49..109
e-value: 2.5E-13
score: 49.9
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 49..112
score: 14.408593
IPR001623DnaJ domainCDDcd06257DnaJcoord: 49..101
e-value: 1.47002E-13
score: 62.9481
NoneNo IPR availableGENE3D3.30.70.20coord: 137..199
e-value: 3.0E-15
score: 58.2
NoneNo IPR availablePFAMPF13370Fer4_13coord: 142..196
e-value: 2.5E-11
score: 43.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..383
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..368
NoneNo IPR availablePANTHERPTHR45295:SF1CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTICcoord: 29..462
NoneNo IPR availablePANTHERPTHR45295CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTICcoord: 29..462
NoneNo IPR availableSUPERFAMILY548624Fe-4S ferredoxinscoord: 139..199
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 36..123
e-value: 4.1E-16
score: 60.9
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 46..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G178390.1Chy10G178390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane