Chy10G177110 (gene) Cucumber (hystrix) v1

Overview
NameChy10G177110
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionTropinone reductase-like protein
LocationchrH10: 11765609 .. 11767927 (+)
RNA-Seq ExpressionChy10G177110
SyntenyChy10G177110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGCCCTGCGGCAGTGCCGGAAAATCACGGTGGTCCCTCGAAGGCTTCACCGCGCTCGTCACCGGCGGCACTCGCGGAATCGGGTCGGCTACTCTCTTTCCTCCGAAACCTTTTTTCTCTTTTGATTCTCTTCTGCTCTCTGTTTACGATTATGTGTCTGTTTTATAGTCACGCTGTGGTGGAGGAACTAGCCGGTTTAGGCGCCTCGGTCCATACCTGCGCCCGGAACGAATCGGACCTTAATCAGTGCTTGAAGGAATGGGAGACCAAGGGTTTTGTGGTAACTGGATCGGTTTGTGATGCTTCTTCAAGAACACAAAGAGAGGAGCTGATACAGGAAGTGGCGTCTTCTTTTAATGGTACTCTCAATATTTTGGTTAGTGAACTTGCTTCTGCCCATTTTGATTTTGGAAAACCATTTGGAGATACTGTTGAAATTGGGTGGTGTTTGTTGCATGGCTGATTTCGGAATTTGAAGCTTTTACATCACATTTTTTTGGTCATTTGCCTTTTAGATTTACTTTCAAATTAGTGGTTGTACAACTTCACGATTCATAATTATTATATGTGTCTTGTCTCTGTCGTTAACTTAAACAAACCATCAATTAGTTGGAATAGCGGAGTTGGGTATTGGGAATGTCAAAAAGTGAAACAATCTGGAAAAATTCATATGAACTTTATAATCTAAGCACCCACCAAATGATATTAAAATATTATCATAGTATTAAGGGCAATGTTAGAGATGAGTTAGGGAAGTTAACATGATTACAAACAGAATGAGGGGGAAGGAAATGATGTAGGTAAGCAATTGTTTAGATGGGTGTGACTTGAGTGTATATGCTCAATAGAGGTGGAAGGTTCTAATTCCAAGTACGTTGTATAATTTTGTTCTAATTCCAATGACAATCACATGTTGTATGTAAATTAGTTTATCAAAAAAGAACAGATGAAATTTATTGATATATGTATGGTTGAACTTTCAGTAAGGTAAAAAGTCCTTCAAAGTGGGACAAACTTTAGTCCTCCTTGTACTTCTAACAAGAATAATGAATTGAAACCACTGTAAAGAGATCTAAATTTTAAAGTAATGTCCTTGTAGGCAGGTGGTCTAATTTTTCTATACTTGATATCCTACACATTGTTTAAGTAAAATATTGATTGAATTGCAATCAAACCATTGAAACTAGAAGCTAGAAAGCCTTCTATATTGACCTCCAGTACTTCAAAGTTTAAAACCCTCCATCTTTTATAGCAACAATGTCTCATCGTTGGTTCGATAATTAATCACATGATCCTTAGGATAGGAAGTTATCTTCCAGGAACTCTGAGAATTTCTTTGTGTAGGTAAACAATGTGGGAACAAACATAAGAAAGCCAAGTGCCGAGTACAGTTTGGAAGAAGTTTCAACATTGATGACAACAAACTTTGAATCTGCATTTCATTTATCCCAACTTTCACATCCTCTTTTAAAAGCTTCTGGAAATGGAAGCATTGTGTTCATTTCTTCTGTTGGTGGTCTTGTGAGCATTGGTAGTGGTTCCATTTATGCTGCAACTAAATGTAAGTGGCTATTTGTAAAACTTAGCTTCTTTTCTTTGGTAGGTTCTTAGAAGCTAAAACTGTTCCCCATTTTTATTTTCTACTCAGCTGCAATAAATCAACTGACCAGAAATCTTACTTGTGAATGGGCCAAGGACAATATCAGGGTCAATTGTGTTGCACCCTGGTATATCAATACCCCTTTTGTAGAAAAGGTAATGTTTTTTCTACCATTCCTGCATAATAATTTCCCTTACATTAGTTTATTTTTGCTCTCATCTTCTTTATCTCTCTTCTAGAAAACAAATGTTGTGCACGGACCAAGGCATGTGGCAGTCCTTTTAATTTTATCCTCCTTTCTCTGAAATATCTGGATATTAAATCAAATCACTTAGGGAACATTTGATAAGCCTACATTTTGATCCTGAATTTTTTTTCCACCAACATTTAGTGACAGAAAATTCTGGAGTTCATATATCTTTCGAGTCTAAAAGTGTCCATCTTTATGGTATGTGTTTTTACCCATAAACCTCTTGAATGTTTAACTAATAACAAGGGTTATTATCACATAGTTGATGAAAAATAAGACGTTGGTGAACAACATAGTGTCTCGAACTCCTCTTGGGCGCATAGGGGAATCAAAGGAAGTGTCGTCGCTGGTGGCATTCCTTTGCTTGCCTGCTGCATCTTACATTACGGGACAGATCATGTCTGTTGATGGAGGATTCACTGCAAATGGATTTGAACCTGGTATGAGACTAGACAAATTGTGA

mRNA sequence

ATGGCGGAGCCCTGCGGCAGTGCCGGAAAATCACGGTGGTCCCTCGAAGGCTTCACCGCGCTCGTCACCGGCGGCACTCGCGGAATCGGTCACGCTGTGGTGGAGGAACTAGCCGGTTTAGGCGCCTCGGTCCATACCTGCGCCCGGAACGAATCGGACCTTAATCAGTGCTTGAAGGAATGGGAGACCAAGGGTTTTGTGGTAACTGGATCGGTTTGTGATGCTTCTTCAAGAACACAAAGAGAGGAGCTGATACAGGAAGTGGCGTCTTCTTTTAATGGTACTCTCAATATTTTGGTAAACAATGTGGGAACAAACATAAGAAAGCCAAGTGCCGAGTACAGTTTGGAAGAAGTTTCAACATTGATGACAACAAACTTTGAATCTGCATTTCATTTATCCCAACTTTCACATCCTCTTTTAAAAGCTTCTGGAAATGGAAGCATTGTGTTCATTTCTTCTGTTGGTGGTCTTGTGAGCATTGGTAGTGGTTCCATTTATGCTGCAACTAAATCTGCAATAAATCAACTGACCAGAAATCTTACTTGTGAATGGGCCAAGGACAATATCAGGGTCAATTGTGTTGCACCCTGGTATATCAATACCCCTTTTGTAGAAAAGTTGATGAAAAATAAGACGTTGGTGAACAACATAGTGTCTCGAACTCCTCTTGGGCGCATAGGGGAATCAAAGGAAGTGTCGTCGCTGGTGGCATTCCTTTGCTTGCCTGCTGCATCTTACATTACGGGACAGATCATGTCTGTTGATGGAGGATTCACTGCAAATGGATTTGAACCTGGTATGAGACTAGACAAATTGTGA

Coding sequence (CDS)

ATGGCGGAGCCCTGCGGCAGTGCCGGAAAATCACGGTGGTCCCTCGAAGGCTTCACCGCGCTCGTCACCGGCGGCACTCGCGGAATCGGTCACGCTGTGGTGGAGGAACTAGCCGGTTTAGGCGCCTCGGTCCATACCTGCGCCCGGAACGAATCGGACCTTAATCAGTGCTTGAAGGAATGGGAGACCAAGGGTTTTGTGGTAACTGGATCGGTTTGTGATGCTTCTTCAAGAACACAAAGAGAGGAGCTGATACAGGAAGTGGCGTCTTCTTTTAATGGTACTCTCAATATTTTGGTAAACAATGTGGGAACAAACATAAGAAAGCCAAGTGCCGAGTACAGTTTGGAAGAAGTTTCAACATTGATGACAACAAACTTTGAATCTGCATTTCATTTATCCCAACTTTCACATCCTCTTTTAAAAGCTTCTGGAAATGGAAGCATTGTGTTCATTTCTTCTGTTGGTGGTCTTGTGAGCATTGGTAGTGGTTCCATTTATGCTGCAACTAAATCTGCAATAAATCAACTGACCAGAAATCTTACTTGTGAATGGGCCAAGGACAATATCAGGGTCAATTGTGTTGCACCCTGGTATATCAATACCCCTTTTGTAGAAAAGTTGATGAAAAATAAGACGTTGGTGAACAACATAGTGTCTCGAACTCCTCTTGGGCGCATAGGGGAATCAAAGGAAGTGTCGTCGCTGGTGGCATTCCTTTGCTTGCCTGCTGCATCTTACATTACGGGACAGATCATGTCTGTTGATGGAGGATTCACTGCAAATGGATTTGAACCTGGTATGAGACTAGACAAATTGTGA

Protein sequence

MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYITGQIMSVDGGFTANGFEPGMRLDKL*
Homology
BLAST of Chy10G177110 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-104
Identity = 185/258 (71.71%), Postives = 214/258 (82.95%), Query Frame = 0

Query: 12  RWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVTGS 71
           RWSL G TALVTGGTRGIG AVVEELA  GA VHTC+RN+ +LN CL +W+  G VV+GS
Sbjct: 6   RWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGS 65

Query: 72  VCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFESAF 131
           VCDAS R QRE+LIQE +S+F+G LNIL+NNVGTN+RKP+ EYS EE + +M+TN ESAF
Sbjct: 66  VCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAF 125

Query: 132 HLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDNIR 191
           HLSQ++HPLLKASG GSIVFISSV GLV + SGSIY ATK A+NQLTRNL CEWA DNIR
Sbjct: 126 HLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIR 185

Query: 192 VNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYITGQ 251
            NCVAPWYI T  VE L++ K  V  +VSRTPLGR+GE +EVSSLVAFLCLPA+SYITGQ
Sbjct: 186 TNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQ 245

Query: 252 IMSVDGGFTANGFEPGMR 270
           ++SVDGGFT NGF   M+
Sbjct: 246 VISVDGGFTVNGFSYAMK 263

BLAST of Chy10G177110 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 5.5e-94
Identity = 169/257 (65.76%), Postives = 202/257 (78.60%), Query Frame = 0

Query: 10  KSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVT 69
           K RWSL G +ALVTGGTRGIG A+VEELAGLGA VHTCARNE +L  CL +W   GF V 
Sbjct: 63  KERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVA 122

Query: 70  GSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFES 129
           GSVCD S R+QRE L++ V+S F G L+ILVNNVGTNIRKP  E++  E STLM+TNFES
Sbjct: 123 GSVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFES 182

Query: 130 AFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDN 189
            FHL QL++PLL+ S  GS+VFISSV G VS+ + S+ ++TK AINQLTR+L CEWAKDN
Sbjct: 183 VFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDN 242

Query: 190 IRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYIT 249
           IR+N VAPWYI T  VE+++ NK  +  + S TPLGR+GE +EVSS VAFLCLPA+SYIT
Sbjct: 243 IRINAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYIT 302

Query: 250 GQIMSVDGGFTANGFEP 267
           GQI+ VDGG + NGF P
Sbjct: 303 GQILCVDGGMSINGFFP 319

BLAST of Chy10G177110 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 8.2e-90
Identity = 161/253 (63.64%), Postives = 196/253 (77.47%), Query Frame = 0

Query: 12  RWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVTGS 71
           RWSL+G  ALVTGGT+GIG AVVEEL+ LGA VHTCAR+E+ L + L+EW+ KGF VT S
Sbjct: 4   RWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTS 63

Query: 72  VCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFESAF 131
           +CD S R QRE+L++ V+S F G LNILVNNVGT + KP+ EY+ EE S LM TN +SAF
Sbjct: 64  ICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAF 123

Query: 132 HLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDNIR 191
           H+SQL+HPLLKASG+GSIV +SS+ G+V +G GSIY ATK A+NQL RNL CEWA DNIR
Sbjct: 124 HISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIR 183

Query: 192 VNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYITGQ 251
            N + PW I TP +  L+  + +      RTP+GR+GE+ EVS LVAFLCLPAASYITGQ
Sbjct: 184 TNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQ 243

Query: 252 IMSVDGGFTANGF 265
           ++ VDGG T NGF
Sbjct: 244 VICVDGGLTVNGF 256

BLAST of Chy10G177110 vs. ExPASy Swiss-Prot
Match: Q9ZW04 (Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g29170 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-89
Identity = 164/255 (64.31%), Postives = 197/255 (77.25%), Query Frame = 0

Query: 10  KSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVT 69
           K RWSL G TALVTGG++G+G AVVEELA LGA VHTCARNE+ L +C++EW+ KGF VT
Sbjct: 11  KCRWSLGGMTALVTGGSKGLGEAVVEELAMLGARVHTCARNETQLQECVREWQAKGFEVT 70

Query: 70  GSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFES 129
            SVCD SSR QRE+L++ VAS F G LNILVNN GT I KP+ EY+ ++ S LM TN +S
Sbjct: 71  TSVCDVSSRDQREKLMENVASIFQGKLNILVNNAGTGITKPTTEYTAQDYSFLMATNLDS 130

Query: 130 AFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDN 189
           AFHLSQL+HPLLKASG+GSIV +SS  G+V I  GSIY ATK A+NQL +NL CEWA+DN
Sbjct: 131 AFHLSQLAHPLLKASGSGSIVLMSSTAGVVHINVGSIYGATKGAMNQLAKNLACEWARDN 190

Query: 190 IRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYIT 249
           IRVN V PW+I TP     + ++ L   +  +TP+GR+G + EVSSLVAFLC PAASYIT
Sbjct: 191 IRVNSVCPWFIATPL---YLNDEELKKEVERKTPMGRVGNANEVSSLVAFLCFPAASYIT 250

Query: 250 GQIMSVDGGFTANGF 265
           GQ + VDGGFT N F
Sbjct: 251 GQTICVDGGFTVNCF 262

BLAST of Chy10G177110 vs. ExPASy Swiss-Prot
Match: Q9ZW03 (Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g29150 PE=1 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.4e-89
Identity = 168/258 (65.12%), Postives = 200/258 (77.52%), Query Frame = 0

Query: 7   SAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGF 66
           S  KSRWSLEG TALVTGG++G+G AVVEELA LGA VHTCAR+E+ L + L+EW+ KGF
Sbjct: 8   SRDKSRWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGF 67

Query: 67  VVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTN 126
            VT SVCD SSR QRE+L++ V+S F G LNILVNN GT I KPS EY+ E+ S LM TN
Sbjct: 68  EVTTSVCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATN 127

Query: 127 FESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWA 186
            ESAFHLSQ++HPLLKASG+GSIVF+SSV GLV  G+ SIY A+K A+NQL R+L CEWA
Sbjct: 128 LESAFHLSQIAHPLLKASGSGSIVFMSSVAGLVHTGA-SIYGASKGAMNQLGRSLACEWA 187

Query: 187 KDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAAS 246
            DNIRVN V PW I TP    +  ++ L   +  +TP+GR+GE+ EVSSLVAFLC PAAS
Sbjct: 188 SDNIRVNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAAS 247

Query: 247 YITGQIMSVDGGFTANGF 265
           YITGQ + VDGG + NGF
Sbjct: 248 YITGQTICVDGGASVNGF 264

BLAST of Chy10G177110 vs. ExPASy TrEMBL
Match: A0A0A0KS92 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G184270 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.2e-145
Identity = 266/273 (97.44%), Postives = 268/273 (98.17%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE  GSAG SRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE
Sbjct: 1   MAEASGSAGNSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KG+VVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS
Sbjct: 61  WEAKGYVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN
Sbjct: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           LTCEWAKDNIRVNCVAPWYINTP VEKLMKNKTLV+NIVSRTPLGRIGESKEVSSLVAFL
Sbjct: 181 LTCEWAKDNIRVNCVAPWYINTPLVEKLMKNKTLVDNIVSRTPLGRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of Chy10G177110 vs. ExPASy TrEMBL
Match: A0A5D3BV45 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00720 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.0e-136
Identity = 250/273 (91.58%), Postives = 260/273 (95.24%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE   SAG  RWSL+GFTALVTGGTRGIGHAVVEELAGLGASVHTC+RN+SDLNQCLKE
Sbjct: 1   MAEASDSAGIPRWSLKGFTALVTGGTRGIGHAVVEELAGLGASVHTCSRNQSDLNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNN GTNIRKP+AEYSLE+VS
Sbjct: 61  WEAKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNAGTNIRKPTAEYSLEDVS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFESA+HLSQLSHPLLKASGNGSIVFISSVGGLVS+GSGSIYAATKSAINQLT+ 
Sbjct: 121 TLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLVSVGSGSIYAATKSAINQLTKY 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIRVN VAPWYINTP VEKL+ NKTLV+NIVSRTPL RIGESKEVSSLVAFL
Sbjct: 181 LACEWAKDNIRVNGVAPWYINTPLVEKLLNNKTLVDNIVSRTPLRRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of Chy10G177110 vs. ExPASy TrEMBL
Match: A0A1S3CF70 (tropinone reductase homolog At5g06060-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500241 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.0e-136
Identity = 250/273 (91.58%), Postives = 260/273 (95.24%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE   SAG  RWSL+GFTALVTGGTRGIGHAVVEELAGLGASVHTC+RN+SDLNQCLKE
Sbjct: 1   MAEASDSAGIPRWSLKGFTALVTGGTRGIGHAVVEELAGLGASVHTCSRNQSDLNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNN GTNIRKP+AEYSLE+VS
Sbjct: 61  WEAKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNAGTNIRKPTAEYSLEDVS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFESA+HLSQLSHPLLKASGNGSIVFISSVGGLVS+GSGSIYAATKSAINQLT+ 
Sbjct: 121 TLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLVSVGSGSIYAATKSAINQLTKY 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIRVN VAPWYINTP VEKL+ NKTLV+NIVSRTPL RIGESKEVSSLVAFL
Sbjct: 181 LACEWAKDNIRVNGVAPWYINTPLVEKLLNNKTLVDNIVSRTPLRRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of Chy10G177110 vs. ExPASy TrEMBL
Match: A0A6J1IKI1 (tropinone reductase homolog At5g06060 OS=Cucurbita maxima OX=3661 GN=LOC111477082 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 4.6e-128
Identity = 234/273 (85.71%), Postives = 252/273 (92.31%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE   S+G SRWSL+GFTALVTGGTRGIG AVVEELAGLGASVHTC+RNE +LNQCLKE
Sbjct: 1   MAEAGVSSGNSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVV+GSVCDASSRTQRE+L+QEVAS+FNGTLNILVNNVGTNIRKPS EYSLEE S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREKLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEYS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFES FHLSQL+HPLLKASGNGSIVF+SSVGGLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIR N VAPWY NT  VEKL+ NKT V+ +VSRTPLGRIGES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTSLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 274
           C+PAASYITGQI+SVDGGFTANGFEPGMRLD L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFEPGMRLDSL 273

BLAST of Chy10G177110 vs. ExPASy TrEMBL
Match: A0A6J1GWZ5 (tropinone reductase homolog At5g06060 OS=Cucurbita moschata OX=3662 GN=LOC111458319 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.1e-126
Identity = 231/273 (84.62%), Postives = 250/273 (91.58%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE   S+G +RWSL+GFTALVTGGTRGIG AVVEELAGLG SVHTC+RNE +LNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVV+GSVCDASSRTQRE L+QEVAS+FNGTLNILVNNVGTNIRKPS EYSLEE S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFES FHLSQL+HPLLKASGNGSIVF+SSVGGLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIR N VAPWY NTP VEKL+ NKT V+ +VSRTPLGRIGES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 274
           C+PAASYITGQI+SVDGGFTANGFE GMRL+ L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 273

BLAST of Chy10G177110 vs. NCBI nr
Match: XP_004150641.1 (tropinone reductase homolog At5g06060 [Cucumis sativus] >KGN50576.1 hypothetical protein Csa_021491 [Cucumis sativus])

HSP 1 Score: 526 bits (1354), Expect = 7.35e-188
Identity = 266/273 (97.44%), Postives = 268/273 (98.17%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE  GSAG SRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE
Sbjct: 1   MAEASGSAGNSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KG+VVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS
Sbjct: 61  WEAKGYVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN
Sbjct: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           LTCEWAKDNIRVNCVAPWYINTP VEKLMKNKTLV+NIVSRTPLGRIGESKEVSSLVAFL
Sbjct: 181 LTCEWAKDNIRVNCVAPWYINTPLVEKLMKNKTLVDNIVSRTPLGRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273
           CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of Chy10G177110 vs. NCBI nr
Match: XP_008461702.1 (PREDICTED: tropinone reductase homolog At5g06060-like isoform X1 [Cucumis melo] >KAA0050360.1 tropinone reductase-like protein [Cucumis melo var. makuwa] >TYK03573.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 495 bits (1274), Expect = 1.15e-175
Identity = 250/273 (91.58%), Postives = 260/273 (95.24%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE   SAG  RWSL+GFTALVTGGTRGIGHAVVEELAGLGASVHTC+RN+SDLNQCLKE
Sbjct: 1   MAEASDSAGIPRWSLKGFTALVTGGTRGIGHAVVEELAGLGASVHTCSRNQSDLNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNN GTNIRKP+AEYSLE+VS
Sbjct: 61  WEAKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNAGTNIRKPTAEYSLEDVS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFESA+HLSQLSHPLLKASGNGSIVFISSVGGLVS+GSGSIYAATKSAINQLT+ 
Sbjct: 121 TLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLVSVGSGSIYAATKSAINQLTKY 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIRVN VAPWYINTP VEKL+ NKTLV+NIVSRTPL RIGESKEVSSLVAFL
Sbjct: 181 LACEWAKDNIRVNGVAPWYINTPLVEKLLNNKTLVDNIVSRTPLRRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273
           CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of Chy10G177110 vs. NCBI nr
Match: XP_038889667.1 (tropinone reductase homolog At5g06060-like [Benincasa hispida])

HSP 1 Score: 482 bits (1240), Expect = 1.74e-170
Identity = 244/273 (89.38%), Postives = 256/273 (93.77%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           M E   S+GKSRWSL+G TALVTGGTRGIGHA+VEELAGLGASVHTC+RNESDLN+CLKE
Sbjct: 1   MTEAGDSSGKSRWSLKGCTALVTGGTRGIGHALVEELAGLGASVHTCSRNESDLNRCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVV+GSVCDASSRTQREELIQEVAS F GTLNILVNNVGTNIRKPS EYSLEEVS
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREELIQEVASVFCGTLNILVNNVGTNIRKPSTEYSLEEVS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFESA+HLSQLSHPLLKASGNGSIVFI+SV GLVSIGSGSIYAATKSAINQLTRN
Sbjct: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFITSVAGLVSIGSGSIYAATKSAINQLTRN 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIR+N VAPWYINTP VEKL+ NKTLV+ IVSRTPLGRIGES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLSNKTLVDKIVSRTPLGRIGESDEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273
           CLPAASYITGQI+SVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIISVDGGFTANGFEPGMRLDKL 273

BLAST of Chy10G177110 vs. NCBI nr
Match: XP_022976820.1 (tropinone reductase homolog At5g06060 [Cucurbita maxima])

HSP 1 Score: 469 bits (1206), Expect = 2.64e-165
Identity = 234/273 (85.71%), Postives = 252/273 (92.31%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE   S+G SRWSL+GFTALVTGGTRGIG AVVEELAGLGASVHTC+RNE +LNQCLKE
Sbjct: 1   MAEAGVSSGNSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVV+GSVCDASSRTQRE+L+QEVAS+FNGTLNILVNNVGTNIRKPS EYSLEE S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREKLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEYS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFES FHLSQL+HPLLKASGNGSIVF+SSVGGLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIR N VAPWY NT  VEKL+ NKT V+ +VSRTPLGRIGES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTSLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273
           C+PAASYITGQI+SVDGGFTANGFEPGMRLD L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFEPGMRLDSL 273

BLAST of Chy10G177110 vs. NCBI nr
Match: KAG6600980.1 (Tropinone reductase-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031592.1 Tropinone reductase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 467 bits (1202), Expect = 1.07e-164
Identity = 234/273 (85.71%), Postives = 250/273 (91.58%), Query Frame = 0

Query: 1   MAEPCGSAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60
           MAE   S+G +RWSL+GFTALVTGGTRGIG AVVEELAGLGASVHTC+RNE +LNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WETKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120
           WE KGFVV GSVCDASSRTQRE L+QEVAS+FNGTLNILVNNVGTNIRKPS EYSLEE S
Sbjct: 61  WEGKGFVVGGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180
           TLMTTNFES FHLSQLSHPLLKASGNGSIVF+SSVGGLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESVFHLSQLSHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LTCEWAKDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFL 240
           L CEWAKDNIR N VAPWY NTP VEKL+ NKT V+ +VSRTPLGRIGES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273
           C+PAASYITGQI+SVDGGFTANGFE GMRLD L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLDSL 273

BLAST of Chy10G177110 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.1e-105
Identity = 185/258 (71.71%), Postives = 214/258 (82.95%), Query Frame = 0

Query: 12  RWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVTGS 71
           RWSL G TALVTGGTRGIG AVVEELA  GA VHTC+RN+ +LN CL +W+  G VV+GS
Sbjct: 6   RWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGS 65

Query: 72  VCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFESAF 131
           VCDAS R QRE+LIQE +S+F+G LNIL+NNVGTN+RKP+ EYS EE + +M+TN ESAF
Sbjct: 66  VCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAF 125

Query: 132 HLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDNIR 191
           HLSQ++HPLLKASG GSIVFISSV GLV + SGSIY ATK A+NQLTRNL CEWA DNIR
Sbjct: 126 HLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIR 185

Query: 192 VNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYITGQ 251
            NCVAPWYI T  VE L++ K  V  +VSRTPLGR+GE +EVSSLVAFLCLPA+SYITGQ
Sbjct: 186 TNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQ 245

Query: 252 IMSVDGGFTANGFEPGMR 270
           ++SVDGGFT NGF   M+
Sbjct: 246 VISVDGGFTVNGFSYAMK 263

BLAST of Chy10G177110 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 3.9e-95
Identity = 169/257 (65.76%), Postives = 202/257 (78.60%), Query Frame = 0

Query: 10  KSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVT 69
           K RWSL G +ALVTGGTRGIG A+VEELAGLGA VHTCARNE +L  CL +W   GF V 
Sbjct: 63  KERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVA 122

Query: 70  GSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFES 129
           GSVCD S R+QRE L++ V+S F G L+ILVNNVGTNIRKP  E++  E STLM+TNFES
Sbjct: 123 GSVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFES 182

Query: 130 AFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDN 189
            FHL QL++PLL+ S  GS+VFISSV G VS+ + S+ ++TK AINQLTR+L CEWAKDN
Sbjct: 183 VFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDN 242

Query: 190 IRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYIT 249
           IR+N VAPWYI T  VE+++ NK  +  + S TPLGR+GE +EVSS VAFLCLPA+SYIT
Sbjct: 243 IRINAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYIT 302

Query: 250 GQIMSVDGGFTANGFEP 267
           GQI+ VDGG + NGF P
Sbjct: 303 GQILCVDGGMSINGFFP 319

BLAST of Chy10G177110 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 5.8e-91
Identity = 161/253 (63.64%), Postives = 196/253 (77.47%), Query Frame = 0

Query: 12  RWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVTGS 71
           RWSL+G  ALVTGGT+GIG AVVEEL+ LGA VHTCAR+E+ L + L+EW+ KGF VT S
Sbjct: 4   RWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTS 63

Query: 72  VCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFESAF 131
           +CD S R QRE+L++ V+S F G LNILVNNVGT + KP+ EY+ EE S LM TN +SAF
Sbjct: 64  ICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAF 123

Query: 132 HLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDNIR 191
           H+SQL+HPLLKASG+GSIV +SS+ G+V +G GSIY ATK A+NQL RNL CEWA DNIR
Sbjct: 124 HISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIR 183

Query: 192 VNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYITGQ 251
            N + PW I TP +  L+  + +      RTP+GR+GE+ EVS LVAFLCLPAASYITGQ
Sbjct: 184 TNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQ 243

Query: 252 IMSVDGGFTANGF 265
           ++ VDGG T NGF
Sbjct: 244 VICVDGGLTVNGF 256

BLAST of Chy10G177110 vs. TAIR 10
Match: AT2G29150.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 9.9e-91
Identity = 168/258 (65.12%), Postives = 200/258 (77.52%), Query Frame = 0

Query: 7   SAGKSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGF 66
           S  KSRWSLEG TALVTGG++G+G AVVEELA LGA VHTCAR+E+ L + L+EW+ KGF
Sbjct: 8   SRDKSRWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGF 67

Query: 67  VVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTN 126
            VT SVCD SSR QRE+L++ V+S F G LNILVNN GT I KPS EY+ E+ S LM TN
Sbjct: 68  EVTTSVCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATN 127

Query: 127 FESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWA 186
            ESAFHLSQ++HPLLKASG+GSIVF+SSV GLV  G+ SIY A+K A+NQL R+L CEWA
Sbjct: 128 LESAFHLSQIAHPLLKASGSGSIVFMSSVAGLVHTGA-SIYGASKGAMNQLGRSLACEWA 187

Query: 187 KDNIRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAAS 246
            DNIRVN V PW I TP    +  ++ L   +  +TP+GR+GE+ EVSSLVAFLC PAAS
Sbjct: 188 SDNIRVNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAAS 247

Query: 247 YITGQIMSVDGGFTANGF 265
           YITGQ + VDGG + NGF
Sbjct: 248 YITGQTICVDGGASVNGF 264

BLAST of Chy10G177110 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 8.4e-90
Identity = 162/255 (63.53%), Postives = 196/255 (76.86%), Query Frame = 0

Query: 10  KSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKEWETKGFVVT 69
           K RWSL G TALVTGG++GIG AVVEELA LGA +HTCAR+E+ L + L++W+ KGF VT
Sbjct: 11  KPRWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVT 70

Query: 70  GSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVSTLMTTNFES 129
            SVCD SSR +RE+L++ V++ F G LNILVNNVGT I KP+ +++ E+ S  M TN ES
Sbjct: 71  TSVCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLES 130

Query: 130 AFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRNLTCEWAKDN 189
           AFHLSQL+HPLLKASG+GSIV ISSV G+V +   SIY  +K A+NQL RNL CEWA DN
Sbjct: 131 AFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDN 190

Query: 190 IRVNCVAPWYINTPFVEKLMKNKTLVNNIVSRTPLGRIGESKEVSSLVAFLCLPAASYIT 249
           IR N V PW+I TP V + + N+     + SR P+GR+GE  EVSSLVAFLCLPAASYIT
Sbjct: 191 IRTNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYIT 250

Query: 250 GQIMSVDGGFTANGF 265
           GQ + VDGGFT NGF
Sbjct: 251 GQTICVDGGFTVNGF 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHT01.5e-10471.71Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060... [more]
Q9ZW125.5e-9465.76Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9ZW138.2e-9063.64Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
Q9ZW041.1e-8964.31Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Q9ZW031.4e-8965.12Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Match NameE-valueIdentityDescription
A0A0A0KS923.2e-14597.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G184270 PE=4 SV=1[more]
A0A5D3BV456.0e-13691.58Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CF706.0e-13691.58tropinone reductase homolog At5g06060-like isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1IKI14.6e-12885.71tropinone reductase homolog At5g06060 OS=Cucurbita maxima OX=3661 GN=LOC11147708... [more]
A0A6J1GWZ51.1e-12684.62tropinone reductase homolog At5g06060 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
Match NameE-valueIdentityDescription
XP_004150641.17.35e-18897.44tropinone reductase homolog At5g06060 [Cucumis sativus] >KGN50576.1 hypothetical... [more]
XP_008461702.11.15e-17591.58PREDICTED: tropinone reductase homolog At5g06060-like isoform X1 [Cucumis melo] ... [more]
XP_038889667.11.74e-17089.38tropinone reductase homolog At5g06060-like [Benincasa hispida][more]
XP_022976820.12.64e-16585.71tropinone reductase homolog At5g06060 [Cucurbita maxima][more]
KAG6600980.11.07e-16485.71Tropinone reductase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT5G06060.11.1e-10571.71NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29260.13.9e-9565.76NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29290.25.8e-9163.64NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29150.19.9e-9165.12NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.18.4e-9063.53NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 19..36
score: 48.79
coord: 94..105
score: 49.49
coord: 167..186
score: 37.54
coord: 223..243
score: 33.53
coord: 188..205
score: 42.05
coord: 141..157
score: 35.11
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 94..105
score: 45.16
coord: 167..186
score: 33.09
coord: 147..155
score: 53.59
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 26..260
e-value: 7.4E-58
score: 195.9
NoneNo IPR availableGENE3D3.40.50.720coord: 5..264
e-value: 2.1E-80
score: 271.7
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 21..213
e-value: 1.1E-19
score: 68.6
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 15..264
e-value: 1.5E-17
score: 61.5
NoneNo IPR availablePANTHERPTHR42898:SF69NAD(P)-BINDING ROSSMANN-FOLD PROTEINcoord: 5..267
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 5..267
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 17..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G177110.1Chy10G177110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity