Chy10G175650 (gene) Cucumber (hystrix) v1

Overview
NameChy10G175650
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
LocationchrH10: 9095711 .. 9098343 (+)
RNA-Seq ExpressionChy10G175650
SyntenyChy10G175650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCTGTATCGTGATGGTGGTGGTAACTCTGGGTGCTTGTTGCACGGTTTCTGTTTCTTTGTTGTTTCATACATGTTTTTCTACAGTATTCTAAATGTCTTGACATTCAGTGGCCTTCTCATCTCACAAGTCAGTTTGAAATCAGTAATTGGACACCTCTTTTCATTCTGTAGATTCATTGACTGTTTCTGTGAAGCAAAAGCCTCCAGCAGAACATCTTTGTGATGAATTTGATGAAGATGAGGATGACCTGGTATGAACCACTAAAACTCAATTGTTGATTTTGGACTGGCAGCTCAGCTTGAAGCCTTTCTAAATTGTTTTTTTCACATGGATGCTTGCATTTTCAGAAGTTTAGGCTTCCAGAGTCTTCAGGTTTTTTGACGAAGAAAGTCTACACTTTTATACGTGAGAAGTTGAAGTTTCTTGGTAATAATCATATCAAATTATGCAAAGAAGTTCAACGTATAAATTGGCTAATTGCATTTTGAGTTCTCTGTTCTGTGAATAGATATCCTTTTGATGGTGGTTTTCTCTCTCAGTCACAAGGGTTGGGCTGCTATTCTCATCTGGTTTATCATGGCACCTGTTGCCCATAGCTGGGACCTTGGACCTTTATATGTAAGTAATGATGTTCTGTTGGTTAGATTTTTAATGTTGGTGTCACTATTTTCAATTTTTCAGTAGTTGATATTTGATGGTAACCTTAGGAGCTGTTTCATAAACTGTTCTGCAACGCTTAGTTTTTCAAGGAGTTCTGTAACATAATTTACAAGTGCCTCCTTGAATATGGTTTACTATGCATTCATTGTTGTCCAATTGTGTTGTTGGAAAAACTAGTTTCAATTGTTCTAAAAAAACTCATGTCTCAAAGAATAAATCAAGAGGAATCAAATTTGGAAAATAGTTGCTTGATGTTTCAACTTAGGGATTTGATGTGAAACACCTTTGTTTTTATATCAATTCTTAAAAGGCCTCTCTCTCTCTTTTTCTCCTTTTTTTTTTTCTTGAAAATTTTTGGGAAATAGATACTACTGCAACTTTGTGTAGGGTCGTCTTTCAGCAAGTGTGAAAATCTCTGTTCTCCATATCCTTTATTAAGCTGATGTAACTTGGTCTCGTATATGATGAAAAATGTATGCATTGGATGGAAAACTAGTTTGTTTTTCTTTTAAATTCACATGCCACAACATCAAAATATGTGCTTTTTTAGATGATTAAAAAATTTATGATAAACTATGTAGCAAGATTGCTATGCACGAGTCCTATTTGCAGATGGAACTGGTTTTTGCATCATTCTACTAATTCTTGGTCATCGACGATCTGGGGGAAATGAGGTACTTGTACTTGATATAAACTCTCATTCCATTCCTTATACCTCATCTGTAGCAGCACTTTTGATTGCACTTGATAAACTAAGAGAATACATGATTGCATGTCTGTATATTTTCCTGTCTCATTTGAGGTATTTTGCTTTTCTTTTTTGGTAAGAAACAATGCTTTTCTTTATATGCAAATTTCTAGTGTTCCTTTATTATCTATTGAATTGTTAGTTTCTATCACATGTTGTATAGTGTTCCTCATTTGTTCATTCAAACTCAGACTCAAATCTTTGTTTCTGTTTTCATCATTACTAATTGTCTCTTATCATGCTTTTCTTCCCCTATATAAATCCCCATTCCCAAACCTGGTTAGATACAATCACTTGCTTCCATAGTTGCTCTACATTACTTAATTCACTCTACTTTCTTCTTCACAATGGCCCAGGAGAGCTCTGTTGTTCCAACAACATTTTCCGCACCTCCTCCTCCTCCATTTAAACCCAATTTAACCCTCTATTCCTATTGCTTATCTTATCAAAACACTCAGGGACCTGGAGTCCAGGGTCTACTTCAATTCATTTCACTACCCATTTAATGTATCCACTGTTCCTCCTCAACCCATGCCATTGCCTGATAGTCGTAGGATTCTTGTTTGCCATGATATGGCTGGTGGATATATAAATGATAAATGGGTTCAGGGTGGAAATAACCCAGATGCTTATGCAATATGGCATTGGCATTTGATGGATATTTTTGTTTACTTTTCCCATTATTTGGTCACACTTCCACCTCCATCTTGGACAAATGCGGCTCACAGGCACGGTGTTAAGGTACCCTATCTATTACCTACTCACTTCACTCATTGCTTTTACTACTCCATACTATACTTCTTTGTTTGGTTATTATTAATTATTATTTGAAAGGTGCTGATGGTACCATGATAACTAAAACAAGTGCTTCTCTAGCATTTACAAATTAATGGTGGTATCGAATCTATAGGTACTTGGAACTTTCATTCTGGAAGGAGGGTGAGAAGCTGTTCGGGATACCTTGCTTTCATCAAAGGATTCTGCTGAATTGTTTGCAGAGTGCTTAGCCGAGCTTTCTATTGCATTGGGATGGATGACTGGTATTGCTTAATAAAGTTTTCCTACCCAGACTGCATTTTTTGTGACTTCATCGAAATGTTTTCATATGAATCATAGAGCTTATTAGGTGTAAATACTTCTTTACAACTTATTTTTTCTCCTGTTCTCCTTTGTAGTTGAATATGGAATCCCTTTGAGTGTCAGCAAGTTACTCATGTGA

mRNA sequence

ATGAATCTGTATCGTGATGGTGGTGGTAACTCTGGGTGCTTGTTGCACGATTCATTGACTGTTTCTGTGAAGCAAAAGCCTCCAGCAGAACATCTTTGTGATGAATTTGATGAAGATGAGGATGACCTGAAGTTTAGGCTTCCAGAGTCTTCAGGTTTTTTGACGAAGAAAGTCTACACTTTTATACGTGAGAAGTTGAAGTTTCTTGATATCCTTTTGATGGTGGTTTTCTCTCTCAGTCACAAGGGTTGGGCTGCTATTCTCATCTGGTTTATCATGGCACCTGTTGCCCATAGCTGGGACCTTGGACCTTTATATCAAGATTGCTATGCACGAGTCCTATTTGCAGATGGAACTGGTTTTTGCATCATTCTACTAATTCTTGGTCATCGACGATCTGGGGGAAATGAGGTACTTGTACTTGATATAAACTCTCATTCCATTCCTTATACCTCATCTGTAGCAGCACTTTTGATTGCACTTGATAAACTAAGAGAATACATGATTGCATGTCTGTATATTTTCCTGTCTCATTTGAGGGACCTGGAGTCCAGGGTCTACTTCAATTCATTTCACTACCCATTTAATGTATCCACTGTTCCTCCTCAACCCATGCCATTGCCTGATAGTCGTAGGATTCTTGTTTGCCATGATATGGCTGGTGGATATATAAATGATAAATGGGTTCAGGGTGGAAATAACCCAGATGCTTATGCAATATGGCATTGGCATTTGATGGATATTTTTGTTTACTTTTCCCATTATTTGGTCACACTTCCACCTCCATCTTGGACAAATGCGGCTCACAGGCACGGTGTTAAGGATTCTGCTGAATTGTTTGCAGAGTGCTTAGCCGAGCTTTCTATTGCATTGGGATGGATGACTGTTGAATATGGAATCCCTTTGAGTGTCAGCAAGTTACTCATGTGA

Coding sequence (CDS)

ATGAATCTGTATCGTGATGGTGGTGGTAACTCTGGGTGCTTGTTGCACGATTCATTGACTGTTTCTGTGAAGCAAAAGCCTCCAGCAGAACATCTTTGTGATGAATTTGATGAAGATGAGGATGACCTGAAGTTTAGGCTTCCAGAGTCTTCAGGTTTTTTGACGAAGAAAGTCTACACTTTTATACGTGAGAAGTTGAAGTTTCTTGATATCCTTTTGATGGTGGTTTTCTCTCTCAGTCACAAGGGTTGGGCTGCTATTCTCATCTGGTTTATCATGGCACCTGTTGCCCATAGCTGGGACCTTGGACCTTTATATCAAGATTGCTATGCACGAGTCCTATTTGCAGATGGAACTGGTTTTTGCATCATTCTACTAATTCTTGGTCATCGACGATCTGGGGGAAATGAGGTACTTGTACTTGATATAAACTCTCATTCCATTCCTTATACCTCATCTGTAGCAGCACTTTTGATTGCACTTGATAAACTAAGAGAATACATGATTGCATGTCTGTATATTTTCCTGTCTCATTTGAGGGACCTGGAGTCCAGGGTCTACTTCAATTCATTTCACTACCCATTTAATGTATCCACTGTTCCTCCTCAACCCATGCCATTGCCTGATAGTCGTAGGATTCTTGTTTGCCATGATATGGCTGGTGGATATATAAATGATAAATGGGTTCAGGGTGGAAATAACCCAGATGCTTATGCAATATGGCATTGGCATTTGATGGATATTTTTGTTTACTTTTCCCATTATTTGGTCACACTTCCACCTCCATCTTGGACAAATGCGGCTCACAGGCACGGTGTTAAGGATTCTGCTGAATTGTTTGCAGAGTGCTTAGCCGAGCTTTCTATTGCATTGGGATGGATGACTGTTGAATATGGAATCCCTTTGAGTGTCAGCAAGTTACTCATGTGA

Protein sequence

MNLYRDGGGNSGCLLHDSLTVSVKQKPPAEHLCDEFDEDEDDLKFRLPESSGFLTKKVYTFIREKLKFLDILLMVVFSLSHKGWAAILIWFIMAPVAHSWDLGPLYQDCYARVLFADGTGFCIILLILGHRRSGGNEVLVLDINSHSIPYTSSVAALLIALDKLREYMIACLYIFLSHLRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAYAIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGVKDSAELFAECLAELSIALGWMTVEYGIPLSVSKLLM*
Homology
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BLAST of Chy10G175650 vs. ExPASy Swiss-Prot
Match: F4JZC2 (Cytosolic endo-beta-N-acetylglucosaminidase 1 OS=Arabidopsis thaliana OX=3702 GN=ENGASE1 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.8e-37
Identity = 78/140 (55.71%), Postives = 94/140 (67.14%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPM--PLPDSRRILVCHDMAGGYINDKWVQGGNNPD 238
           L+DL+SR YF+SFHYPFN S+VP +     L D  R+LVCHDM GGY++DKWVQG  N  
Sbjct: 29  LQDLKSRSYFDSFHYPFNRSSVPLRRNIGALSDRPRLLVCHDMKGGYVDDKWVQGCGNNA 88

Query: 239 AYAIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------- 294
            YAIW W+LMD+FVYFSH LVTLPPP WTN AHRHGV                       
Sbjct: 89  GYAIWDWYLMDVFVYFSHSLVTLPPPCWTNTAHRHGVKVLGTFITEWDEGKATCKELLAT 148

[Close]
BLAST of Chy10G175650 vs. ExPASy Swiss-Prot
Match: Q9SRL4 (Cytosolic endo-beta-N-acetylglucosaminidase 2 OS=Arabidopsis thaliana OX=3702 GN=ENGASE2 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.8e-37
Identity = 77/140 (55.00%), Postives = 95/140 (67.86%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMP--LPDSRRILVCHDMAGGYINDKWVQGGNNPD 238
           L+DL+SR YF+SFH+ FN STVP +     LP+  R+LVCHDM GGY++DKWVQG  N  
Sbjct: 34  LQDLKSRSYFDSFHFQFNRSTVPFRRNSDCLPNRPRVLVCHDMKGGYVDDKWVQGCENEA 93

Query: 239 AYAIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------- 294
            +AIWHW+LMDIFVYFSH LVT+PPP WTN AHRHGV                       
Sbjct: 94  GFAIWHWYLMDIFVYFSHSLVTIPPPCWTNTAHRHGVKVLGTFITEWDEGKATCKEMLAT 153

[Close]
BLAST of Chy10G175650 vs. ExPASy Swiss-Prot
Match: Q8BX80 (Cytosolic endo-beta-N-acetylglucosaminidase OS=Mus musculus OX=10090 GN=Engase PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.9e-20
Identity = 41/73 (56.16%), Postives = 52/73 (71.23%), Query Frame = 0 

Query: 201 PPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAYAIWHWHLMDIFVYFSHYLVTLP 260
           PP   P P   R L+CHDM GGY+ D+++QG    + Y+ +HW  +DIFVYFSH+ VT+P
Sbjct: 108 PPLSSPRP---RTLLCHDMMGGYLEDRFIQGSEVQNPYSFYHWQYIDIFVYFSHHTVTIP 167

Query: 261 PPSWTNAAHRHGV 274
           P  WTNAAHRHGV
Sbjct: 168 PVCWTNAAHRHGV 177

[Close]
BLAST of Chy10G175650 vs. ExPASy Swiss-Prot
Match: P0C7A1 (Cytosolic endo-beta-N-acetylglucosaminidase OS=Gallus gallus OX=9031 GN=ENGASE PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.7e-20
Identity = 51/102 (50.00%), Postives = 64/102 (62.75%), Query Frame = 0 

Query: 175 FLSHLRDLESRVYFNSFHYPFNVSTVP-PQPMPLPDSR--RILVCHDMAGGYINDKWVQG 234
           FLS L +L    +  S    FNV  VP  Q  P   SR  R L+CHDM GGY+ D+++QG
Sbjct: 84  FLSGLEEL--LAWHPSSDDEFNVCAVPLAQRQPPLHSRRPRTLLCHDMRGGYLEDRFIQG 143

Query: 235 GNNPDAYAIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV 274
               + Y  +HW  +DIFVYFSH+ VT+PP  WTNAAHR+GV
Sbjct: 144 SATRNPYVFYHWRYVDIFVYFSHHTVTIPPVCWTNAAHRNGV 183

[Close]
BLAST of Chy10G175650 vs. ExPASy Swiss-Prot
Match: A1L251 (Cytosolic endo-beta-N-acetylglucosaminidase OS=Danio rerio OX=7955 GN=engase PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-19
Identity = 46/82 (56.10%), Postives = 56/82 (68.29%), Query Frame = 0 

Query: 195 FNVSTVP--PQPMPLPD-SRRILVCHDMAGGYINDKWVQGGNNPDAYAIWHWHLMDIFVY 254
           FNVS+VP   +  PL    RR LV HDM GGY+ D+++QG      YA +HW  +DIF Y
Sbjct: 82  FNVSSVPLASRYPPLESCPRRTLVSHDMMGGYLEDRFIQGAEVETPYAFYHWEYIDIFNY 141

Query: 255 FSHYLVTLPPPSWTNAAHRHGV 274
           FSH +VT+PP  WTNAAHRHGV
Sbjct: 142 FSHQMVTIPPAVWTNAAHRHGV 163

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BLAST of Chy10G175650 vs. ExPASy TrEMBL
Match: A0A5A7SX49 (Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold529G00270 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 2.8e-49
Identity = 97/149 (65.10%), Postives = 110/149 (73.83%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L+DLESR YFNSFHYPFN+STVP QP PLPD RRILVCHDMAGGYI+DKW+QGG NPDAY
Sbjct: 36  LKDLESRAYFNSFHYPFNISTVPLQPAPLPDRRRILVCHDMAGGYIDDKWIQGGTNPDAY 95

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------KDS 298
           AIWHW+L+D+FVYFSH LVTLPPP WTN AHRHGV                      KDS
Sbjct: 96  AIWHWYLIDVFVYFSHDLVTLPPPLWTNTAHRHGVKVLGTFIVEGGGTDVRDTLLSSKDS 155

Query: 299 AELFAECLAELSIALGWMTVEYGIPLSVS 306
           AE++AE L ELS+ALG+      + +SVS
Sbjct: 156 AEMYAERLTELSMALGFDGWLLNMEISVS 184

[Close]
BLAST of Chy10G175650 vs. ExPASy TrEMBL
Match: A0A1S3BZ17 (Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucumis melo OX=3656 GN=LOC103495146 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 2.8e-49
Identity = 97/149 (65.10%), Postives = 110/149 (73.83%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L+DLESR YFNSFHYPFN+STVP QP PLPD RRILVCHDMAGGYI+DKW+QGG NPDAY
Sbjct: 36  LKDLESRAYFNSFHYPFNISTVPLQPAPLPDRRRILVCHDMAGGYIDDKWIQGGTNPDAY 95

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------KDS 298
           AIWHW+L+D+FVYFSH LVTLPPP WTN AHRHGV                      KDS
Sbjct: 96  AIWHWYLIDVFVYFSHDLVTLPPPLWTNTAHRHGVKVLGTFIVEGGGTDVRDTLLSSKDS 155

Query: 299 AELFAECLAELSIALGWMTVEYGIPLSVS 306
           AE++AE L ELS+ALG+      + +SVS
Sbjct: 156 AEMYAERLTELSMALGFDGWLLNMEISVS 184

[Close]
BLAST of Chy10G175650 vs. ExPASy TrEMBL
Match: A0A6J1IQC7 (Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucurbita maxima OX=3661 GN=LOC111477230 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 7.2e-45
Identity = 89/137 (64.96%), Postives = 102/137 (74.45%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L DLESRVYF SFHYPFN STV  QP+PLPD RR+LVCHDMAGGY +DKWVQGG NPDA+
Sbjct: 32  LEDLESRVYFKSFHYPFNRSTVALQPVPLPDRRRMLVCHDMAGGYKDDKWVQGGTNPDAF 91

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------KDS 294
           AIWHW+L+D+FVYFSH LVTLPPP WTN AHRHGV                      K+S
Sbjct: 92  AIWHWYLIDVFVYFSHDLVTLPPPCWTNTAHRHGVKVLGTFILEGGGEAARDTLLSTKES 151

[Close]
BLAST of Chy10G175650 vs. ExPASy TrEMBL
Match: A0A6J1GZD6 (Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucurbita moschata OX=3662 GN=LOC111458519 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 7.2e-45
Identity = 89/137 (64.96%), Postives = 101/137 (73.72%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L DLESR YF SFHYPFN STV  QP+PLPD RR+LVCHDMAGGY +DKWVQGG NPDAY
Sbjct: 32  LEDLESRAYFKSFHYPFNRSTVALQPVPLPDRRRMLVCHDMAGGYKDDKWVQGGTNPDAY 91

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGVK----------------------DS 294
           AIWHW+L+D+FVYFSH LVTLPPP WTN AHRHGVK                      +S
Sbjct: 92  AIWHWYLIDVFVYFSHDLVTLPPPCWTNTAHRHGVKVLGTFILEGGGEAARDTLLSTNES 151

[Close]
BLAST of Chy10G175650 vs. ExPASy TrEMBL
Match: A0A6J1GY36 (Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucurbita moschata OX=3662 GN=LOC111458520 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.2e-44
Identity = 87/137 (63.50%), Postives = 101/137 (73.72%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L +LESR YF SFHYPFNVSTV   P  LP+ RR+LVCHDMAGGY++DKWVQGG+NPDAY
Sbjct: 23  LAELESRAYFKSFHYPFNVSTVALPPASLPNRRRMLVCHDMAGGYLDDKWVQGGSNPDAY 82

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------KDS 294
           AIWHW+LMD+F+YFSH LVTLPPP WTN AHRHGV                      KDS
Sbjct: 83  AIWHWYLMDVFMYFSHDLVTLPPPVWTNTAHRHGVKVLGTFIVEGGGKDVRDTLLASKDS 142

[Close]
BLAST of Chy10G175650 vs. NCBI nr
Match: XP_038876449.1 (cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X3 [Benincasa hispida] >XP_038876450.1 cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X3 [Benincasa hispida] >XP_038876451.1 cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X3 [Benincasa hispida] >XP_038876452.1 cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 202 bits (515), Expect = 2.50e-59
Identity = 96/147 (65.31%), Postives = 107/147 (72.79%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L DLESR YFNSFHYPFN+STVP QP PLPD RRILVCHDMAGGYI+DKWVQGG NPDAY
Sbjct: 34  LDDLESRAYFNSFHYPFNISTVPLQPAPLPDRRRILVCHDMAGGYIDDKWVQGGTNPDAY 93

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGVK----------------------DS 298
           AIWHW+L+D+FVYFSH LVTLPPP WTN AHRHGVK                      DS
Sbjct: 94  AIWHWYLIDVFVYFSHDLVTLPPPCWTNTAHRHGVKVLGTFILEEGGTDVRDTLLSSKDS 153

[Close]
BLAST of Chy10G175650 vs. NCBI nr
Match: XP_038876447.1 (cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 202 bits (515), Expect = 2.01e-58
Identity = 96/147 (65.31%), Postives = 107/147 (72.79%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L DLESR YFNSFHYPFN+STVP QP PLPD RRILVCHDMAGGYI+DKWVQGG NPDAY
Sbjct: 111 LDDLESRAYFNSFHYPFNISTVPLQPAPLPDRRRILVCHDMAGGYIDDKWVQGGTNPDAY 170

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGVK----------------------DS 298
           AIWHW+L+D+FVYFSH LVTLPPP WTN AHRHGVK                      DS
Sbjct: 171 AIWHWYLIDVFVYFSHDLVTLPPPCWTNTAHRHGVKVLGTFILEEGGTDVRDTLLSSKDS 230

[Close]
BLAST of Chy10G175650 vs. NCBI nr
Match: XP_038877781.1 (LOW QUALITY PROTEIN: cytosolic endo-beta-N-acetylglucosaminidase 1-like [Benincasa hispida])

HSP 1 Score: 210 bits (535), Expect = 2.65e-58
Identity = 97/137 (70.80%), Postives = 106/137 (77.37%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           LRDLESR YFNSFHYPFN+STVP QP PLPD RRILVCHDM GGY++DKWVQGG NPDAY
Sbjct: 117 LRDLESRAYFNSFHYPFNISTVPLQPAPLPDRRRILVCHDMKGGYVDDKWVQGGTNPDAY 176

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGVK----------------------DS 293
           AIWHWHL+DIFVYFSH LVTLPPP WTN AHRHGVK                      DS
Sbjct: 177 AIWHWHLIDIFVYFSHDLVTLPPPCWTNTAHRHGVKVLGTFIVEGGGEHVRDTLLSSKDS 236

[Close]
BLAST of Chy10G175650 vs. NCBI nr
Match: XP_038876453.1 (cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X4 [Benincasa hispida] >XP_038876454.1 cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X4 [Benincasa hispida] >XP_038876455.1 cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X4 [Benincasa hispida])

HSP 1 Score: 196 bits (499), Expect = 2.62e-57
Identity = 91/145 (62.76%), Postives = 105/145 (72.41%), Query Frame = 0 

Query: 181 DLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAYAI 240
            +++R YFNSFHYPFN+STVP QP PLPD RRILVCHDMAGGYI+DKWVQGG NPDAYAI
Sbjct: 6   SVQTRAYFNSFHYPFNISTVPLQPAPLPDRRRILVCHDMAGGYIDDKWVQGGTNPDAYAI 65

Query: 241 WHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGVK----------------------DSAE 299
           WHW+L+D+FVYFSH LVTLPPP WTN AHRHGVK                      DSAE
Sbjct: 66  WHWYLIDVFVYFSHDLVTLPPPCWTNTAHRHGVKVLGTFILEEGGTDVRDTLLSSKDSAE 125

[Close]
BLAST of Chy10G175650 vs. NCBI nr
Match: XP_004148186.3 (cytosolic endo-beta-N-acetylglucosaminidase 1 [Cucumis sativus] >KGN50312.2 hypothetical protein Csa_005893 [Cucumis sativus])

HSP 1 Score: 207 bits (527), Expect = 3.35e-57
Identity = 99/149 (66.44%), Postives = 111/149 (74.50%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMPLPDSRRILVCHDMAGGYINDKWVQGGNNPDAY 238
           L+DLESR YFNSFHYPFN+STVP QP PLPD RRILVCHDMAGGYI+DKWVQGG NPDAY
Sbjct: 109 LKDLESRAYFNSFHYPFNISTVPLQPAPLPDRRRILVCHDMAGGYIDDKWVQGGTNPDAY 168

Query: 239 AIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGVK----------------------DS 298
           AIWHW+L+D+FVYFSH LVTLPPP WTN AHRHGVK                      DS
Sbjct: 169 AIWHWYLIDVFVYFSHDLVTLPPPCWTNTAHRHGVKVLGTFIVEGGGTDVRDTLLSSKDS 228

Query: 299 AELFAECLAELSIALGWMTVEYGIPLSVS 305
           AE++AE LAELS+ALG+      + +SVS
Sbjct: 229 AEMYAERLAELSMALGFDGWLLNMEISVS 257

[Close]
BLAST of Chy10G175650 vs. TAIR 10
Match: AT3G11040.1 (Glycosyl hydrolase family 85 )

HSP 1 Score: 157.9 bits (398), Expect = 1.3e-38
Identity = 77/140 (55.00%), Postives = 95/140 (67.86%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPMP--LPDSRRILVCHDMAGGYINDKWVQGGNNPD 238
           L+DL+SR YF+SFH+ FN STVP +     LP+  R+LVCHDM GGY++DKWVQG  N  
Sbjct: 34  LQDLKSRSYFDSFHFQFNRSTVPFRRNSDCLPNRPRVLVCHDMKGGYVDDKWVQGCENEA 93

Query: 239 AYAIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------- 294
            +AIWHW+LMDIFVYFSH LVT+PPP WTN AHRHGV                       
Sbjct: 94  GFAIWHWYLMDIFVYFSHSLVTIPPPCWTNTAHRHGVKVLGTFITEWDEGKATCKEMLAT 153

[Close]
BLAST of Chy10G175650 vs. TAIR 10
Match: AT5G05460.1 (Glycosyl hydrolase family 85 )

HSP 1 Score: 157.9 bits (398), Expect = 1.3e-38
Identity = 78/140 (55.71%), Postives = 94/140 (67.14%), Query Frame = 0 

Query: 179 LRDLESRVYFNSFHYPFNVSTVPPQPM--PLPDSRRILVCHDMAGGYINDKWVQGGNNPD 238
           L+DL+SR YF+SFHYPFN S+VP +     L D  R+LVCHDM GGY++DKWVQG  N  
Sbjct: 29  LQDLKSRSYFDSFHYPFNRSSVPLRRNIGALSDRPRLLVCHDMKGGYVDDKWVQGCGNNA 88

Query: 239 AYAIWHWHLMDIFVYFSHYLVTLPPPSWTNAAHRHGV----------------------- 294
            YAIW W+LMD+FVYFSH LVTLPPP WTN AHRHGV                       
Sbjct: 89  GYAIWDWYLMDVFVYFSHSLVTLPPPCWTNTAHRHGVKVLGTFITEWDEGKATCKELLAT 148

[Close]
BLAST of Chy10G175650 vs. TAIR 10
Match: AT2G35360.1 (ubiquitin family protein )

HSP 1 Score: 109.0 bits (271), Expect = 6.9e-24
Identity = 64/133 (48.12%), Postives = 84/133 (63.16%), Query Frame = 0 

Query: 17  DSLTVSVKQKPPAEHLCDEF-DEDEDDLKFRLPESSGFLTKKVYTFIREKLKFLDILLMV 76
           DS  V+V   PPA    + F D+D+DDLKF+LP S+    +K+Y F+R KLK  DI+LM 
Sbjct: 85  DSFIVAVIPNPPAG--VESFDDDDDDDLKFKLPPSASRWKRKLYYFLRNKLKLPDIILMG 144

Query: 77  VFSLSHKGWAAILIWFIMAPVAHSWDLGPLYQDCYARVLFADGTGFCIILLILGHRRSGG 136
           +FSLS K W  I +WFI+AP+AH WDLGP         +F  GTGF IILL LG R+ G 
Sbjct: 145 LFSLSLKMWVIITLWFILAPIAHRWDLGP---------IFILGTGFSIILLNLGKRQPG- 199

Query: 137 NEVLVLDINSHSI 149
                 D++++SI
Sbjct: 205 ------DVSAYSI 199

The following BLAST results are available for this feature:
BLAST of Chy10G175650 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4JZC21.8e-3755.71Cytosolic endo-beta-N-acetylglucosaminidase 1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SRL41.8e-3755.00Cytosolic endo-beta-N-acetylglucosaminidase 2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8BX805.9e-2056.16Cytosolic endo-beta-N-acetylglucosaminidase OS=Mus musculus OX=10090 GN=Engase P... [more]
P0C7A17.7e-2050.00Cytosolic endo-beta-N-acetylglucosaminidase OS=Gallus gallus OX=9031 GN=ENGASE P... [more]
A1L2511.0e-1956.10Cytosolic endo-beta-N-acetylglucosaminidase OS=Danio rerio OX=7955 GN=engase PE=... [more]
BLAST of Chy10G175650 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7SX492.8e-4965.10Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucumis melo var. mak... [more]
A0A1S3BZ172.8e-4965.10Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucumis melo OX=3656 ... [more]
A0A6J1IQC77.2e-4564.96Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucurbita maxima OX=3... [more]
A0A6J1GZD67.2e-4564.96Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucurbita moschata OX... [more]
A0A6J1GY361.2e-4463.50Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cucurbita moschata OX... [more]
BLAST of Chy10G175650 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038876449.12.50e-5965.31cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X3 [Benincasa hispida... [more]
XP_038876447.12.01e-5865.31cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X1 [Benincasa hispida... [more]
XP_038877781.12.65e-5870.80LOW QUALITY PROTEIN: cytosolic endo-beta-N-acetylglucosaminidase 1-like [Beninca... [more]
XP_038876453.12.62e-5762.76cytosolic endo-beta-N-acetylglucosaminidase 1-like isoform X4 [Benincasa hispida... [more]
XP_004148186.33.35e-5766.44cytosolic endo-beta-N-acetylglucosaminidase 1 [Cucumis sativus] >KGN50312.2 hypo... [more]
BLAST of Chy10G175650 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G11040.11.3e-3855.00Glycosyl hydrolase family 85 [more]
AT5G05460.11.3e-3855.71Glycosyl hydrolase family 85 [more]
AT2G35360.16.9e-2448.12ubiquitin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005201Glycoside hydrolase, family 85PFAMPF03644Glyco_hydro_85coord: 224..282
e-value: 4.2E-18
score: 65.8
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 169..288
e-value: 2.6E-28
score: 101.2
NoneNo IPR availablePANTHERPTHR13246:SF4BETA N-ACETYLGLUCOSAMINIDASE, PUTATIVE-RELATEDcoord: 177..275
IPR032979Cytosolic endo-beta-N-acetylglucosaminidasePANTHERPTHR13246ENDO BETA N-ACETYLGLUCOSAMINIDASEcoord: 177..275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G175650.1Chy10G175650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0033925 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity