Chy10G175160 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G175160
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Location	chrH10: 7989168 .. 7992241 (-)
RNA-Seq Expression	Chy10G175160
Synteny	Chy10G175160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAACCGTAGCGCCAAGAATCCTATCTCCTTCCCCGTTTCTTCACATCCCACAAACAAACTCCCATTTCCTCTCCTCTTCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAATATCCCCCAACAATTGTCTGTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTCTACCGGAAATCTAAGGGCCACCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCAGAGGGTTATTGTGAAGAATCTATCGAGAGTTGCGGAAACTTTTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGTGGACATCGGCCTTCTCAGTGAGTGTAGAATCTTACACTTCTAGTTATTTGCTTTAACCGGTTCATTAAAATCTTGATATGAAATGTCTTATCATTGTAATATCTTGTATCTATATATAGAGGTGCTGAGGGATTGATAATGGTTATAATTAAATTTAACAATAACTCAATAGTTTAAGCTGTTGGGTCATGATTAGATATAGTATAGAGTTTAATTAGATATGGTATAAAGTTGATGTGTTTAAACTCTGTAATGTACTAAGGTCCTTCCCACCTAATATTGTTTTTCACTTTTGTTGGGAATTATATAAATTTTCAAGTCGGATAAGTGTGGTGGAGTGTTATTGTTATATTTTTTTTTTTTTTTTTTGAAAAGGAGACAAAGTGCTATTGTTATAATTTAATTTATCTTGAATTTGGTGTTAGCTCTCAATTAATAGTGTTAGTTACTTGGTGAGTCTTTGTCTTTAGGACTAAGCAATTCGACTTGAAACTGAAGGATTGGTTAAACAAAAACTTAAAACTGACATTTTAGCTGAATGTTTTAAAACCCTGAACTGAACTAGCTAAATTCACAAAACCAGCTGAAATTGATTAAGGGTTGCTAAAAATAACAAACTTTACCCAAATCATTCTGATTGTTTCTGGTTTTGACTTCCATCCTTACTTGTACTAAGTTTTAAGCCTAAAAGTGAAATGGTGGCTAAAATGAATATAGGTGGATAATTATATGAAGATGTGGTAATTAAAATTCTTTTTTCTCCTTTTTCTTGTTTCTCTTTTTTTCTTTCCATTTCTCTCTAGATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGTGATAGTTGATGGGTTTAGTGTTGTCTGCTAGTGTTGGATGTATCTAGTATGATCATTGATGGTGATTGATTGAAGCTCTCACTATTCAATATCAGGCATTATGGTTTTGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGGTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTAAGATAATGACTACTATGGCTTTGCTTGAAATGATTCATGAATATGCTTGGATTTGGTGGTTTCTTATTCATTTAGGTTCTTGCTTTCTAGTGTAATTATTTGCAACATTCAATCTTACAAGTTCGAAGATGCATTGTTTTTGACTTGTTAGGTTTCTGAGTCACTCGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACGTCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAAGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCCAATTTTACTTGTTCTTCTCGTGATTTAATATTTATCTCTTTTTGATCCAATAATGCAAGAAGTGATCTATCAAGGCCCCCCTTCTCTATGCTTAGGTGGCAAGTCGAGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGTGAGAATACTGGTAGTTAAGATGTGATAAATTGTGTGATGCATGTTATTCCACACGACTTAACTGTTTTTGTTGTCGTGATATTCTCTTGAATGCTTGAAAGTAAAGAAATATTGTTGTATACATGAGACCAAGACACAAAAACAATGATGGGTTATTCTCTATGTAGGACATAGCCATGGCGCGGATATGCTTGCTAAAATTTATATGTTTTTGAGCTATATCGTTTTTATACAATTTGAAGTCTGTAAGCTTTGTGTGTAATAAATATCTATTTGAAAAAATAAGTTTGGATATGTTACTTCAATATTTATTATTATTATTTTGTATTATCACTTTGCATATCTGTGTATTAATCTTAACAAATATTTGGCATGTCTAACAAGTGCAAAAACTTATATTCAATTTGTTTAACTACTGTCCAACACATGTCTACTTGTCCTGTGAGGTACAAGTATATCACACCTGTTCATCATGTTTTGGAGTGTCCAATACGCGTTGAACATGGACGATTTGTCCAAACTAGCATGTTTTTTTGCTTGGATCGTAGATGGGTGAAAAGAAAGACAATTCTCTCTTTTCTTTCTCTTTCTGAGGGTTTTGGTTGTGGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAAAATTGCTCAACTGCAGCAAAAGTGTCTTGGTGCGCATCGAGTCACATGA

mRNA sequence

ATGTTAACCGTAGCGCCAAGAATCCTATCTCCTTCCCCGTTTCTTCACATCCCACAAACAAACTCCCATTTCCTCTCCTCTTCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAATATCCCCCAACAATTGTCTGTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTCTACCGGAAATCTAAGGGCCACCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCAGAGGGTTATTGTGAAGAATCTATCGAGAGTTGCGGAAACTTTTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGTGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGCATTATGGTTTTGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGGTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTCGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACGTCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAAGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGAGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAAAATTGCTCAACTGCAGCAAAAGTGTCTTGGTGCGCATCGAGTCACATGA

Coding sequence (CDS)

ATGTTAACCGTAGCGCCAAGAATCCTATCTCCTTCCCCGTTTCTTCACATCCCACAAACAAACTCCCATTTCCTCTCCTCTTCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAATATCCCCCAACAATTGTCTGTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTCTACCGGAAATCTAAGGGCCACCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCAGAGGGTTATTGTGAAGAATCTATCGAGAGTTGCGGAAACTTTTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGTGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGCATTATGGTTTTGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGGTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTCGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACGTCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAAGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGAGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAAAATTGCTCAACTGCAGCAAAAGTGTCTTGGTGCGCATCGAGTCACATGA

Protein sequence

MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDANDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIKIAQLQQKCLGAHRVT*

Homology

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BLAST of Chy10G175160 vs. ExPASy Swiss-Prot
Match: B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.6e-32
Identity = 116/374 (31.02%), Postives = 182/374 (48.66%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VLA     K+ G  RVLDAL   GIR +RY +E+ A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 124 NQRVIVKNL-----------SRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVD 183
             R+IV N+            ++A   G+ +  +VTN DANR+M E +    +FDF+D+D
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNEQF---RYFDFVDLD 146

Query: 184 SFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYVRPMPFSNEIGL 243
            FGS   FL  A+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GL
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGL 206

Query: 244 RMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGN 303
           R+LIG + R A+        L +YY  H   FR  L++  G    ++    + Y  +   
Sbjct: 207 RILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ--- 266

Query: 304 SQGFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQG 363
                  E G+      ++ +       GPLW+GPL + ++VE++  L+ +    S    
Sbjct: 267 ------DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHPLGS---- 326

Query: 364 KDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIAS 423
           K  +K L  + DE +  +P  Y     VA R  +    L+ ++  + + GY A+R+H + 
Sbjct: 327 KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSP 371

Query: 424 NAIKTNCPMAECIK 426
            A+KT+ P  E ++
Sbjct: 387 TALKTDAPFEEVLE 371

[Close]

BLAST of Chy10G175160 vs. ExPASy Swiss-Prot
Match: Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 2.8e-31
Identity = 103/369 (27.91%), Postives = 179/369 (48.51%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ V+   + +       VLDAL   GIR +R+ +E+ A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPR----IVLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 124 NQRVIVKNL-----SRVAETFG-----DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+       + E+ G       +  +V + DANR+M E   K  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 244 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCKKCG 303
           L+G VAR A+        + +YY  H   FR  +++  G    +    + G++ + +  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 304 NSQGFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQ 363
                   E+ +   P   +         GP+W+GPL S + VE++L +++Q   +S  +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 364 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 420
            K   KLL+ + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]

BLAST of Chy10G175160 vs. ExPASy Swiss-Prot
Match: P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 8.1e-31
Identity = 107/376 (28.46%), Postives = 173/376 (46.01%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VL   + +       VLDAL   GIR +R+ +E+ A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPK----IVLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 124 NQRVIVKNL----------SRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+          +     F   +  ++ N DANR+M E   K  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 244 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCKKCG 303
           L+G +AR A+        L +YY  H   FR  +++  G    +    + G+V + +K G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 304 NSQGFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQ 363
             +                S +S      GPLW+GPL +   V ++L L      ++N  
Sbjct: 269 RFE-------------VEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 364 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 423
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 377

Query: 424 SNAIKTNCPMAECIKI 427
              IKTN P    +++
Sbjct: 389 PTGIKTNAPYEVFVEV 377

[Close]

BLAST of Chy10G175160 vs. ExPASy Swiss-Prot
Match: Q97ZH0 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.1e-30
Identity = 105/372 (28.23%), Postives = 191/372 (51.34%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVES-EADFVLANDAND 123
           F+  +    RDL V+  ++ +       ++DAL   GIR +RY VES +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPK----IIVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 124 ENQRVIVKNLSRVAETFG-DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFL 183
               +I  N    A+  G +N +  + N DAN ++ E        D+ID+D FGS + F+
Sbjct: 95  NATSLIQIN----AKNNGIENAK--IYNKDANALLYEVK-----SDYIDIDPFGSPAPFI 154

Query: 184 RPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVARE 243
             ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + RE
Sbjct: 155 LSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIRE 214

Query: 244 ASVLGFYATPLFSYYSYHGPVFRVMLRINRGR----IHKNRHYGFVSYCKKCGNSQGFSW 303
           A++L     PLFS+Y+ +   +R+ +R+  G      + N+H  +   C +CG      +
Sbjct: 215 AAILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG------F 274

Query: 304 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKL 363
               + +C        E   + GPL+IGPL+S ++++ I+ L   + ++++     ++KL
Sbjct: 275 QTFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKL 334

Query: 364 LRQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIKT 423
           L   V E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T
Sbjct: 335 LN--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRT 375

Query: 424 NCPMAE---CIK 426
           +    E   CIK
Sbjct: 395 DKEYEEITKCIK 375

[Close]

BLAST of Chy10G175160 vs. ExPASy Swiss-Prot
Match: Q5JIB3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=trm1 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.8e-29
Identity = 112/376 (29.79%), Postives = 180/376 (47.87%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    +  RD+ VLA     K  G  RVLDAL   GIR +RY +E+ A+ V  ND ++E
Sbjct: 27  FYNPVMSLNRDISVLAV----KVLGPKRVLDALSATGIRGIRYALETPAEEVWLNDISEE 86

Query: 124 NQRVIVKN--LSRVAETFGDNRR---W-----LVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN  L+   E + +  R   W     ++   DANR+M E +    +FDF+D+D 
Sbjct: 87  AYGLMKKNASLNIDGELYEEGDRSYLWGEKLVVINKGDANRLMAENF---RYFDFLDLDP 146

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLR 243
           FGS   FL  A+ +++ +G+L VT+TD G   G +R +  L  Y A     P  +E GLR
Sbjct: 147 FGSPVEFLDTALRSVRRNGVLAVTATDTGVLCGAYR-NACLRKYLAEPIRGPLCHEAGLR 206

Query: 244 MLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNS 303
           +LIG V R A+        L +Y+  H   FR  L++  G               K  + 
Sbjct: 207 ILIGTVVRYAAKYDLGVEVLLAYHRDH--YFRAFLKLKSG----------AKKADKSLSQ 266

Query: 304 QGFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGK 363
            GF W++            + +    SGPLW+GPL    +VE++L  A+          K
Sbjct: 267 LGFLWADKSG-RFEYRRGFLPDKPGASGPLWLGPLKDESFVEELLESARDHPLAH----K 326

Query: 364 DLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASN 423
                L+ + +E D  +P  Y     +A    +  P L  ++  + + G++A+++H +  
Sbjct: 327 KTLSFLQLISEELD--VPFHY-DTHTLARACSLTPPKLDVIIQRLRELGHSATKTHFSPT 374

Query: 424 AIKTNCP---MAECIK 426
           ++KT+ P   +AE +K
Sbjct: 387 SVKTDAPFGVVAEVMK 374

[Close]

BLAST of Chy10G175160 vs. ExPASy TrEMBL
Match: A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.8e-246
Identity = 424/440 (96.36%), Postives = 431/440 (97.95%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTVAPR LSPSPFLHIPQTNSHFL+SSSIHLFQFSHSNKI  NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+RVIVKNLSRVAE  GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYC+KCGNSQ FSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADY+EDILTLAKQWEWI NGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIAQLQQKCLGAHRVT 441
           AECI+IAQLQ+ CLGAH+VT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

[Close]

BLAST of Chy10G175160 vs. ExPASy TrEMBL
Match: A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 7.7e-242
Identity = 414/440 (94.09%), Postives = 427/440 (97.05%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTVA   LSPSPFL+I QTNSHFL+S SIHLFQFSHSNK SPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+RVIVKNLS+VAE FGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYC+KCGNSQ FSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADY+EDILTLAKQWEWI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIAQLQQKCLGAHRVT 441
           A+CI+IAQLQQ CLG H+VT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Chy10G175160 vs. ExPASy TrEMBL
Match: A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 7.7e-242
Identity = 414/440 (94.09%), Postives = 427/440 (97.05%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTVA   LSPSPFL+I QTNSHFL+S SIHLFQFSHSNK SPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+RVIVKNLS+VAE FGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYC+KCGNSQ FSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADY+EDILTLAKQWEWI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIAQLQQKCLGAHRVT 441
           A+CI+IAQLQQ CLG H+VT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Chy10G175160 vs. ExPASy TrEMBL
Match: A0A6J1JBD3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484232 PE=3 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.7e-225
Identity = 392/441 (88.89%), Postives = 412/441 (93.42%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTV    LSPSPFL   QT SH  SSS+IHLFQFS S K   NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+ VIVKNLSRVAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYCKKCGNSQGFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADY+ED+LTLA QWEWI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIA-QLQQKCLGAHRVT 441
           AECI+IA +LQQ CLG H+VT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of Chy10G175160 vs. ExPASy TrEMBL
Match: A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.0e-225
Identity = 391/441 (88.66%), Postives = 410/441 (92.97%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTV    LSPSPFL   QT SH  SSS+IHLFQFS S K   NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+ VIVKNLSRVAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLGFY TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYCKKCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADY+ED+LTLA QWEWI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIA-QLQQKCLGAHRVT 441
           AECI+IA +LQQ CLG H+ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of Chy10G175160 vs. NCBI nr
Match: XP_011655038.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_005829 [Cucumis sativus])

HSP 1 Score: 858 bits (2216), Expect = 1.33e-313
Identity = 424/440 (96.36%), Postives = 431/440 (97.95%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTVAPR LSPSPFLHIPQTNSHFL+SSSIHLFQFSHSNKI  NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+RVIVKNLSRVAE  GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYC+KCGNSQ FSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADY+EDILTLAKQWEWI NGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIAQLQQKCLGAHRVT 440
           AECI+IAQLQ+ CLGAH+VT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

[Close]

BLAST of Chy10G175160 vs. NCBI nr
Match: XP_008456934.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >XP_008456936.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >TYK22417.1 tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 842 bits (2176), Expect = 1.66e-307
Identity = 414/440 (94.09%), Postives = 427/440 (97.05%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTVA   LSPSPFL+I QTNSHFL+S SIHLFQFSHSNK SPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+RVIVKNLS+VAE FGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYC+KCGNSQ FSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADY+EDILTLAKQWEWI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIAQLQQKCLGAHRVT 440
           A+CI+IAQLQQ CLG H+VT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Chy10G175160 vs. NCBI nr
Match: XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 804 bits (2076), Expect = 2.98e-292
Identity = 396/441 (89.80%), Postives = 420/441 (95.24%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           ML+V PR  SPSPFLH PQTNSH LS+S+IHLFQFSHSNK S NNCL NNRTERGL+FDI
Sbjct: 1   MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           G TFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+RVIVKNLSRVAE  GD RRW+VTNCDANRVMT FYL+KDFFDFID+DSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLG YATPLFSYYSYHGPVFRVMLRINRGR++KNRHYGF+SYC+KCGNSQ FSWSEL
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVS+SLVVSGPLW GPLHSADY+E++L+LAKQW+WI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIA-QLQQKCLGAHRVT 440
           AECI+IA +LQQ CLG H+VT
Sbjct: 421 AECIRIALELQQNCLGFHQVT 441

[Close]

BLAST of Chy10G175160 vs. NCBI nr
Match: XP_022986521.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima])

HSP 1 Score: 788 bits (2035), Expect = 5.27e-286
Identity = 392/441 (88.89%), Postives = 412/441 (93.42%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTV    LSPSPFL   QT SH  SSS+IHLFQFS S K   NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+ VIVKNLSRVAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYCKKCGNSQGFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADY+ED+LTLA QWEWI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIA-QLQQKCLGAHRVT 440
           AECI+IA +LQQ CLG H+VT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of Chy10G175160 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 786 bits (2031), Expect = 2.14e-285
Identity = 391/441 (88.66%), Postives = 410/441 (92.97%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCLSNNRTERGLDFDI 60
           MLTV    LSPSPFL   QT SH  SSS+IHLFQFS S K   NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDEN+ VIVKNLSRVAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSEL 300
           EASVLGFY TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYCKKCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADY+ED+LTLA QWEWI NGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIKIA-QLQQKCLGAHRVT 440
           AECI+IA +LQQ CLG H+ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of Chy10G175160 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 552.7 bits (1423), Expect = 2.6e-157
Identity = 268/430 (62.33%), Postives = 339/430 (78.84%), Query Frame = 0


Query: 1   MLTVAPRILSPSPFLHIPQTNSHFLSSSSIHLFQFSHSNKISPNNCL--SNNRTERGLDF 60
           +LT++P+ LS S F  + ++ +    S     F+   S K S +  L  S  + ER L+F
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRFK---SLKCSFDRILVKSEVQIERNLEF 61

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVESEADFVLAN 120
           + G+TFFRHESA GRDLGVL+A LY++S G LRVLDA+CGCGIRSLRYLVE+EADFV+AN
Sbjct: 62  ETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMAN 121

Query: 121 DANDENQRVIVKNLSRVAETFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
           DAND+N+RVI  NLS+V    GD RRW+VT+  AN+ M E Y+  DFFD ID+DSFGSDS
Sbjct: 122 DANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDS 181

Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
           SFLR A NAL+L GLLY+TSTDG+SSGGHRP  SLAAYGA++RPMPF NEIGLRMLIGG 
Sbjct: 182 SFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGA 241

Query: 241 AREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWS 300
            REA++LG++ TPLFSYYSYHGPVFRVMLR++RG++H++R+YGFV++C  CG+S    + 
Sbjct: 242 VREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFD 301

Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLL 360
           ELG M CPC+D++ S SLVVSGP+W+GPLH A YV ++L LAK+W W+S G G DL+KLL
Sbjct: 302 ELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLL 361

Query: 361 RQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
             M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+C
Sbjct: 362 SIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDC 421

Query: 421 PMAECIKIAQ 429
           PM+  ++IA+
Sbjct: 422 PMSHFVRIAK 428

[Close]

BLAST of Chy10G175160 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 84.7 bits (208), Expect = 2.0e-16
Identity = 90/355 (25.35%), Postives = 145/355 (40.85%), Query Frame = 0


Query: 91  RVLDALCGCGIRSLRYLVESEA---DFVLAND--ANDENQRVIVKN----LSRVAETFGD 150
           +VL+AL   G+R+LRY  E E       L ND  + +  QR I  N    +S+V     D
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 151 NRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDG 210
            R  ++T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTH-------------PKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 211 FSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGP 270
               G       + YG+Y     + +E+ LR+L+  +   A+    Y  P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 271 VFRVMLRINRGRIHKNRHYGFVSYCKKCGNSQGFSWSELGQMSCPCNDS----------- 330
             RV +R+             +SY  +C     F    +G+ S P N+S           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 331 -----RVSESLVVSGPLWIGPLHSADYVEDILTLAKQWEWISNGQGKDLEKLLRQMVDES 390
                   +   + GP+W  P+H  ++V  IL   K  +        D    +   V E 
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEE 422

Query: 391 DSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
              +P+ ++ +  + +  K  SP  +   S +I A Y  S +H+    +KT+ PM
Sbjct: 423 LLDVPL-FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

The following BLAST results are available for this feature:

BLAST of Chy10G175160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6YUU9	5.6e-32	31.02	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N...	[more]
Q9V1P3	2.8e-31	27.91	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O...	[more]
P81554	8.1e-31	28.46	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ...	[more]
Q97ZH0	3.1e-30	28.23	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strai...	[more]
Q5JIB3	5.8e-29	29.79	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus kodakarensis (strain...	[more]

BLAST of Chy10G175160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KP99	1.8e-246	96.36	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A1S3C509	7.7e-242	94.09	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
A0A5D3DFE8	7.7e-242	94.09	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1JBD3	1.7e-225	88.89	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1FWD4	5.0e-225	88.66	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of Chy10G175160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655038.1	1.33e-313	96.36	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tR...	[more]
XP_008456934.1	1.66e-307	94.09	PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo]...	[more]
XP_038892632.1	2.98e-292	89.80	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida]	[more]
XP_022986521.1	5.27e-286	88.89	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima]	[more]
XP_022943113.1	2.14e-285	88.66	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata]	[more]

BLAST of Chy10G175160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56330.1	2.6e-157	62.33	N2,N2-dimethylguanosine tRNA methyltransferase	[more]
AT3G02320.1	2.0e-16	25.35	N2,N2-dimethylguanosine tRNA methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 37..367 e-value: 3.0E-74 score: 251.6
None	No IPR available	PANTHER	PTHR10631:SF9	TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE	coord: 11..431
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 91..197 e-value: 7.22373E-4 score: 37.0243
IPR042296	tRNA methyltransferase, Trm1, C-terminal	GENE3D	3.30.56.70		coord: 372..428 e-value: 2.1E-16 score: 61.4
IPR002905	tRNA methyltransferase, Trm1	PFAM	PF02005	TRM	coord: 64..426 e-value: 5.8E-66 score: 223.2
IPR002905	tRNA methyltransferase, Trm1	PANTHER	PTHR10631	N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE	coord: 11..431
IPR002905	tRNA methyltransferase, Trm1	PROSITE	PS51626	SAM_MT_TRM1	coord: 49..427 score: 93.396645
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 52..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G175160.1	Chy10G175160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002940	tRNA N2-guanine methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
molecular_function	GO:0003723	RNA binding