CcUC11G220670 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC11G220670
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptiontRNA pseudouridine synthase
LocationCicolChr11: 25140565 .. 25145037 (+)
RNA-Seq ExpressionCcUC11G220670
SyntenyCcUC11G220670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGAAAACCAGAAGCACGGGCTAGAATTCAGTTTTGATTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACTGCAGACGAAGTTCTCCGCGGCGACGAGGAGGAAGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTTGTCGCAGTCGAATATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGGTTGGCGTGCTTGAGGTACTTTCCCATTTTTTCTTTACAATGCGTCTCATTCTTAACGAATGAAACTCTTTGGAACGGGACACAAGTTCTTTGTATCGGCGCTCTGTTTAGCTGGTTTAAATGTCTTGACAATTTGTAGATTGAGAGACCAACAGGAAGATGAACTTGTACAAGTTACATTTGTTCTTTTTTCTTTTGCTCTTTTAACTTCTTAGTAATCATTGGCAACGAATATGAAACATATAGACTGATATATCGCATTAAGTAACATTTATGAAGGATCTTTACTGTTTGACACTTTTGTATTGCTTCCAAGTTCTATTTCCATATCTGCCTTTGTCGTCATTTAGACGATCTGAAAAGTAAGATTCATTTTTGAACGATGCATCTCCTGAATTACACTATCTAGTACTTTGTTAGGGAACTATTGGGGATTTATGGGTAGGAGTAGTATGCTATTAGTAAGGGCATAAAGATAATTAGCTAGGGAGTGTGTTTAGTTGTAAATAGAGGGAGGGGGATTGAGGGAAGGGAGACAACTGTTGGGTGATTTAGGGCTTGGTAATGCGTACTCAAGAGGGGAGGTTCAAAGTGCCTTATATCTGGTTTATCTCGTAGTTCTTAAGACTTTGTATTTCAATATATTTCAAATTTTGATTACTTTAGGTGGTACCCTAACATATGTTTCTTTCAAAATGATCACCTTACACTTGTCAGGAAGCGTTCCAGAAGTTTATCGGCCAACCAGTCTCAGTGTTTTGTTCCAGCAGAACTGTAAGTTTATCTTATCATGGATAATTGTTCCCTTTTGTCGTACAAAACAATCTTCATGTTTTTATTACTTATTAGAATGTACAGTATACCCGTAGGATATTATTAAACCAGATGAAGATATAACCTATTAAAATTGAAAAGTAGTTCGAAGACAGTTCTTTCTCAAGGTTCCATGATTTTTATCTTGTTGATTCCTTAGTCCTCTGGTAGTTTTTCTCCCACTAGCTTTGATATGCGCAGAGAAGGATATGCAATTGAACTGATAATTTGGCTGTTCGCTTAACGATGTTGAAATTCTTGAACTTATGTTCATTTGAAATAATGGACTTGCCTTTATTGTTCTAAGTCGGATTTTTCAAACAAATCAATTGTGACATTTTAGGATAAGTTGTTTTTCTGGCTTGTTTGTTGCGGAACGCAATGCATCTAGCTCCATATTCGTCTGTTCTAATGTGTGCAAATTAGAAGGGGATTTGTGATGGAGCTGCTCCTTTGATTACATTAATTGTTTGCAAAAGAAAATTAATATTACTTTTGGATTTTCTGCCAAACTGCAACCTGCGTTGTATGCATGTACTATTGTAGTTATGAAGTCTGAGTATATTTTTATTAAAGTTATCAAGTCAATTGCTTTTGCTTTCTTTGATATTTGAATTTCCATGTGTGATTTTTCGTTTGTTTCTAACATAATTATATTAGGACATCCCATTCACCTTGTGTCTTTCCTTCCTTTTTTCAATCAAGGATGCTGGAGTACACGCCTTGTCAAATGTTTGTCATGTTGATGTTGAGCGCTTAAGCAAAAGAAAGCCTGGAGAAGTGGTTTGTCCCTTTGTAATAGAGTATCAGCATCCCTATTCCCTCTCATATGTACACCTATAATGTTTGCTCAACTAGATGAGTGATTTTTCCTGAAAACTTTAGTTCACTAATTGACTTTATTGTTGAGATTACACTCCTTATACATGAAAACATACGAGTTTGAAGAAATGGTGCTCTACTTTGCCTCAGAGATGCTAACTTCTTTTTCCCAACTATGCTGCAGTTACCACCTCATGAACCGGGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGGTTTGGTTTTCTGCTGGCCAATCACTTTTTTCAGCTACTTCTGCTTCTTCTGATATTCGTTATGATCTTGTGCTTCATTTTAAAGTTAAAGATGCTGCATGCAATCGTCCTGATAAAATTGCCATTTCCAATCTATAAATTTTATCTTTCTTTTTGAATTTGCAAGCACGTTAGAATCCTCCCTTTTACCATTACTATTGAGTTTAGATTTAAAACCGCTATATGTCCTTTTACCATTACTATTGACTGTTGTTCAAAGGCTATAGATGAATTTCCTTTTTCCAGCTTGAGTCTTTATTTTTTTATTGCTTAACAAAGAAAATGATGAATGAAAGATATTTGTGTTGGTTTTGATTTCAGAAACATGAAGGTGATGTTACAGTAGTTGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACGTGAGTACGATCCATCCATGCTTTAGTAGTTGATACCATTGTTGCATATTAGATAGTAGAATTGATTGTGCGCACCTTCATAAAACAAGTTTTGCATCTCATGAAATATTTTCAGATACTTTTATCGGTTAGTTTCTGGGCCAGAAGCTCTGTTAAGCTTTGAGAAGGATCGAGCATGGCACGTGCCTGAGACACTGAATATTGGGGCAATGCAGGTATGAACAAATAACAGTTCAATGCTGGAGATCATATAGATTTCGAGTTACATTCCAGAATTGATGCTACATTATTGAACTGTGTAAATTCAGGAGGCATGCAAAGTGCTTGTTGGGCATCATGATTTTAGCTCCTTCAGGGCAGCTGGTTGTCAGGTCACTTAATGCTAAAATTATGGGTAAAATGCACCTTTGATTATGAGGTTTGAGGTTTATGCATGTTTAGTCTTTGAATTTTTTAAACAAACACTTTAGTCCCTCATGTTTGCAAAATGCTTCTACATAGTCTCTGACTTAACTTTAAGTTGACAGAATACTTATTTGAACGTTATGTGATGATGTGGATATTAATTGTATTGATGACATGTGAATTTCCTTTGATTAGTTGTTAAAAGGCCGTCCGCAAACGTAAGGGGTTAAAGTGTTTGTTTTGAAAATTTCAAGGACTAAATAGACATCAACCTAAACCTCAATGACTAAAAGTGCATTTTGTCTTAAAATTATTTATGTTCCTAGCATATTATACTTTTTGTTAGTAACAAAAGCCAGGGTGTGGTCGGGGAATGAAATAGTGTATGGTGCAATGTAAATTAGTTTAGATGTGCATTGTGCAGTGCAGGCAAACTCACCAATCAGAACTCTGGATGAACTAGACGTTTGTGAGGTGGTTTCAACTCCTTATTTTCCAACATCAATGGAGCGAAAGCAAAGGCAGGGCAAAGAGGAAGGTGAGAATGAAGTGCCACATTCAGAAACTTTTGAGGTTGGATTTGGAGTCAGGAGAAGACATCGTTGTTTTGTGGTGACAGCACGAGCACGCTCTTTTCTCTATCATCAGGTTCAAAATCACGTACAATTTTGTACATGTTTGGGAGTGATTTTGAAATTGTTAAAATCACTTTTGTCTGTTTAAAATCACTCTAAAATGCTCCTTAAACCACTTGAAATAACTTTATGCCCCGTCTAGTACCATTTTGTTTTTTGTTTTTTAGTTTTTGAAGATTAAGCCTATAAATACTCTTTTTAGTTTTCACCTCTAAATTTTTTGCTTTATTATATACTATTTATCAATATTTTCAAAAACTAAGTCAAGTTTTGAAAACTCGAAAAAGTAGATCTTAAAAACTTATTTTTTGTTTTAAACTTTGACAAATAATTCAACTCTTTTACTTAAGAAATATACAAATTATTGTATGAAATTGAGAGGAAAATATGCTTAACTTCCAAAAACCGAAAACAAAAATCCAAATGGTTACCAAAATGAGCCTTAATATTTAATTTTACGCTTTTAAATACAATTTTCATATTAGCAAAATTACTTTTGAATCATTAAAACCATTTTTCATCCTGATTTTAACCACCATACATTCCCTTTCTTTTCTTGTTCATGAGATTGATTGATTAGTTATTCTGGAATCGAATATATTTCTTAGGTGAGACTAATGGTTGGAGTTCTTAAAGCGGTTGGCACCGGAGATCTAACCGTAGGAGATGGTAGGTGGCTAGGCAGCAACATATACAACAGCAGTCCTTGTAGCTTTTTCTTTGAATTAATAGAACATCGATGTTTGAAGGTAGAATAATAACATGGTGTGTCTTATTTGGTGCAGTAGAAAGAATCTTGGAAGCGAAGAATGTGAATTCAGCAAGCCCAATGGCTCCTGCTTGCGGACTCTACCTCGGCCATGTCAAATATGATCTACCGCCAGCTTCCCCCGAATGA

mRNA sequence

ATGACGGAAAACCAGAAGCACGGGCTAGAATTCAGTTTTGATTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACTGCAGACGAAGTTCTCCGCGGCGACGAGGAGGAAGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTTGTCGCAGTCGAATATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGGTTGGCGTGCTTGAGGAAGCGTTCCAGAAGTTTATCGGCCAACCAGTCTCAGTGTTTTGTTCCAGCAGAACTGATGCTGGAGTACACGCCTTGTCAAATGTTTGTCATGTTGATGTTGAGCGCTTAAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCGGGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTACAGTAGTTGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACATACTTTTATCGGTTAGTTTCTGGGCCAGAAGCTCTGTTAAGCTTTGAGAAGGATCGAGCATGGCACGTGCCTGAGACACTGAATATTGGGGCAATGCAGGAGGCATGCAAAGTGCTTGTTGGGCATCATGATTTTAGCTCCTTCAGGGCAGCTGGTTGTCAGGCAAACTCACCAATCAGAACTCTGGATGAACTAGACGTTTGTGAGGTGGTTTCAACTCCTTATTTTCCAACATCAATGGAGCGAAAGCAAAGGCAGGGCAAAGAGGAAGGTGAGAATGAAGTGCCACATTCAGAAACTTTTGAGGTTGGATTTGGAGTCAGGAGAAGACATCGTTGTTTTGTGGTGACAGCACGAGCACGCTCTTTTCTCTATCATCAGGTGAGACTAATGGTTGGAGTTCTTAAAGCGGTTGGCACCGGAGATCTAACCGTAGGAGATGTAGAAAGAATCTTGGAAGCGAAGAATGTGAATTCAGCAAGCCCAATGGCTCCTGCTTGCGGACTCTACCTCGGCCATGTCAAATATGATCTACCGCCAGCTTCCCCCGAATGA

Coding sequence (CDS)

ATGACGGAAAACCAGAAGCACGGGCTAGAATTCAGTTTTGATTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACTGCAGACGAAGTTCTCCGCGGCGACGAGGAGGAAGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTTGTCGCAGTCGAATATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGGTTGGCGTGCTTGAGGAAGCGTTCCAGAAGTTTATCGGCCAACCAGTCTCAGTGTTTTGTTCCAGCAGAACTGATGCTGGAGTACACGCCTTGTCAAATGTTTGTCATGTTGATGTTGAGCGCTTAAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCGGGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTACAGTAGTTGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACATACTTTTATCGGTTAGTTTCTGGGCCAGAAGCTCTGTTAAGCTTTGAGAAGGATCGAGCATGGCACGTGCCTGAGACACTGAATATTGGGGCAATGCAGGAGGCATGCAAAGTGCTTGTTGGGCATCATGATTTTAGCTCCTTCAGGGCAGCTGGTTGTCAGGCAAACTCACCAATCAGAACTCTGGATGAACTAGACGTTTGTGAGGTGGTTTCAACTCCTTATTTTCCAACATCAATGGAGCGAAAGCAAAGGCAGGGCAAAGAGGAAGGTGAGAATGAAGTGCCACATTCAGAAACTTTTGAGGTTGGATTTGGAGTCAGGAGAAGACATCGTTGTTTTGTGGTGACAGCACGAGCACGCTCTTTTCTCTATCATCAGGTGAGACTAATGGTTGGAGTTCTTAAAGCGGTTGGCACCGGAGATCTAACCGTAGGAGATGTAGAAAGAATCTTGGAAGCGAAGAATGTGAATTCAGCAAGCCCAATGGCTCCTGCTTGCGGACTCTACCTCGGCCATGTCAAATATGATCTACCGCCAGCTTCCCCCGAATGA

Protein sequence

MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPASPE
Homology
BLAST of CcUC11G220670 vs. NCBI nr
Match: XP_038877867.1 (tRNA pseudouridine synthase A isoform X2 [Benincasa hispida])

HSP 1 Score: 675.2 bits (1741), Expect = 3.0e-190
Identity = 330/350 (94.29%), Postives = 337/350 (96.29%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLEFS DSS QRPLKIGKT DEVL   EEEAE MNRNPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLEFSIDSSPQRPLKIGKTTDEVLNRHEEEAETMNRNPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           FSGSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHV ETLNIGAMQ+ACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV
Sbjct: 181 LSSFEKDRAWHVSETLNIGAMQDACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGK EG++EVPHSET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGK-EGDSEVPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHV+YDLPPA
Sbjct: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVRYDLPPA 349

BLAST of CcUC11G220670 vs. NCBI nr
Match: XP_008445970.1 (PREDICTED: tRNA pseudouridine synthase A isoform X1 [Cucumis melo] >KAA0034150.1 tRNA pseudouridine synthase A isoform X1 [Cucumis melo var. makuwa] >TYK15769.1 tRNA pseudouridine synthase A isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 674.5 bits (1739), Expect = 5.1e-190
Identity = 327/350 (93.43%), Postives = 334/350 (95.43%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLE S DSSSQRPLKIGKT D+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           FSGSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEE ENE PHSET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVGTGDLTVGDVERILEAKNV+SA PMAPACGLYLGHVKYDLPPA
Sbjct: 301 VGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLPPA 350

BLAST of CcUC11G220670 vs. NCBI nr
Match: XP_008445972.1 (PREDICTED: tRNA pseudouridine synthase A isoform X2 [Cucumis melo])

HSP 1 Score: 668.7 bits (1724), Expect = 2.8e-188
Identity = 326/350 (93.14%), Postives = 333/350 (95.14%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLE S DSSSQRPLKIGKT D+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           FSGSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEE ENE PHSET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVGTGDLTVGD ERILEAKNV+SA PMAPACGLYLGHVKYDLPPA
Sbjct: 301 VGVLKAVGTGDLTVGD-ERILEAKNVSSARPMAPACGLYLGHVKYDLPPA 349

BLAST of CcUC11G220670 vs. NCBI nr
Match: XP_038877866.1 (tRNA pseudouridine synthase A isoform X1 [Benincasa hispida])

HSP 1 Score: 666.4 bits (1718), Expect = 1.4e-187
Identity = 330/362 (91.16%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLEFS DSS QRPLKIGKT DEVL   EEEAE MNRNPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLEFSIDSSPQRPLKIGKTTDEVLNRHEEEAETMNRNPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGV------------LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC 120
           FSGSQ+QP CRTVVG             LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC
Sbjct: 61  FSGSQQQPNCRTVVGALEVAEDDVLKMSLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC 120

Query: 121 HVDVERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 180
           HVDVER+SKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER
Sbjct: 121 HVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 180

Query: 181 TYFYRLVSGPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPI 240
           TYFYRLVSGPEAL SFEKDRAWHV ETLNIGAMQ+ACKVLVGHHDFSSFRAAGCQANSPI
Sbjct: 181 TYFYRLVSGPEALSSFEKDRAWHVSETLNIGAMQDACKVLVGHHDFSSFRAAGCQANSPI 240

Query: 241 RTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTAR 300
           RTLDELDVCEVVSTPYFPTSMERKQRQGK EG++EVPHSET EVGFG+RRRHRCFVVTAR
Sbjct: 241 RTLDELDVCEVVSTPYFPTSMERKQRQGK-EGDSEVPHSETCEVGFGIRRRHRCFVVTAR 300

Query: 301 ARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLP 351
           ARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHV+YDLP
Sbjct: 301 ARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVRYDLP 360

BLAST of CcUC11G220670 vs. NCBI nr
Match: XP_011655540.1 (uncharacterized protein LOC101221274 isoform X1 [Cucumis sativus] >KGN51629.1 hypothetical protein Csa_008571 [Cucumis sativus])

HSP 1 Score: 662.5 bits (1708), Expect = 2.0e-186
Identity = 323/350 (92.29%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLE S DSSSQRPLKIGKT DE+  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           F GSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEE E EVP SET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVG+GDLTVGDV RILEAKNV+SA PMAPACGLYLGHVKYDLPPA
Sbjct: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPA 350

BLAST of CcUC11G220670 vs. ExPASy Swiss-Prot
Match: Q2WAT0 (tRNA pseudouridine synthase A OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=truA PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.5e-46
Identity = 116/297 (39.06%), Postives = 154/297 (51.85%), Query Frame = 0

Query: 49  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC 108
           RY + VEY GT F+G Q+Q    +V G+LE+A +K  G P ++  + RTDAGVHA   V 
Sbjct: 3   RYRLLVEYDGTPFNGWQRQDKGLSVQGILEKAVEKLCGVPCTLHAAGRTDAGVHATGQVA 62

Query: 109 HVDVERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 168
           HVD+ R            +    V  A+N+ ++     V VV    V  DFHAR+ A  R
Sbjct: 63  HVDLPR-----------DYPADTVRDALNYHMKPK--PVAVVAAELVDEDFHARFSAVGR 122

Query: 169 TYFYRLVSGPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPI 228
            Y YR+V+   A L+ ++ RAW VP  L+  AM E  + L+GHHDFS+FRA+ CQA SP+
Sbjct: 123 AYLYRIVN-RRAPLALDQHRAWWVPVALDAEAMAEGARRLLGHHDFSTFRASECQAKSPM 182

Query: 229 RTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTAR 288
           +TLD LDV  V                G+E                          + A 
Sbjct: 183 KTLDVLDVTRV----------------GEE------------------------IRIVAE 242

Query: 289 ARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKY 346
           ARSFL+HQVR MVG LK VG G  +  D+ ++LEA++     P APA GL L  V Y
Sbjct: 243 ARSFLHHQVRNMVGTLKLVGEGKWSPDDMAKVLEARDRTKGGPTAPAAGLVLTGVSY 245

BLAST of CcUC11G220670 vs. ExPASy Swiss-Prot
Match: B6IPI0 (tRNA pseudouridine synthase A OS=Rhodospirillum centenum (strain ATCC 51521 / SW) OX=414684 GN=truA PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.2e-46
Identity = 120/299 (40.13%), Postives = 152/299 (50.84%), Query Frame = 0

Query: 47  IQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSN 106
           +QR+ + +EY G  F G Q+Q    +V   LEEA  +F  + V+VFC+ RTDAGVHAL+ 
Sbjct: 1   MQRWKLTLEYDGRPFVGWQRQDNGPSVQQALEEAIYRFTQETVTVFCAGRTDAGVHALAM 60

Query: 107 VCHVDVERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQ 166
           V  VD+ R +               V  A+N  L+ H   V V+ V  V  DFHAR+   
Sbjct: 61  VASVDLAREATADK-----------VQAALNFHLKPH--PVAVLRVEPVAPDFHARFSCL 120

Query: 167 ERTYFYRLVSGPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANS 226
            R Y YR+++   A  + E  R WHV   L+  AM EA + LVG HDFSSFRA+ CQA S
Sbjct: 121 GRAYLYRILN-RRAPAALEAGRVWHVQGGLDAEAMHEAAQRLVGQHDFSSFRASLCQAKS 180

Query: 227 PIRTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVT 286
           P++TL +L V                                   VG  VR       + 
Sbjct: 181 PVKTLSDLSVA---------------------------------RVGEEVR-------IV 240

Query: 287 ARARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKY 346
           ARARSFL+HQVR MVG LK VG G  T  DV R L A+N ++A P APA GLY     Y
Sbjct: 241 ARARSFLHHQVRNMVGTLKLVGEGRWTADDVSRALAARNRSAAGPTAPAAGLYFTEAWY 245

BLAST of CcUC11G220670 vs. ExPASy Swiss-Prot
Match: A7IKI9 (tRNA pseudouridine synthase A OS=Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) OX=78245 GN=truA PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.6e-45
Identity = 115/298 (38.59%), Postives = 154/298 (51.68%), Query Frame = 0

Query: 49  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC 108
           RY + +EY GT ++G Q Q   +TV G L +AF++F G+ V V  + RTDAGVHA   V 
Sbjct: 3   RYKLTIEYDGTPYAGWQIQADQQTVQGALAQAFKRFCGEDVHVAGAGRTDAGVHATGQVA 62

Query: 109 HVDVERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 168
           HVD+ R                 V  A+   L+ H   + V+    VP+ F AR+ A +R
Sbjct: 63  HVDLSR-----------HWRSDTVRDAMTAQLRPH--PIAVLSAEEVPDSFDARFSATKR 122

Query: 169 TYFYRLVS-GPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSP 228
            Y YR+++  P+  L+ ++DRAW +P+ L+  AM EA + L+G HDFS+FRAA CQA SP
Sbjct: 123 HYLYRIINRRPD--LALDRDRAWRIPQKLDAQAMHEAAQRLLGKHDFSTFRAAECQAASP 182

Query: 229 IRTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTA 288
           ++TLD+LDV                 R G E                          V  
Sbjct: 183 LKTLDQLDV----------------SRSGDE------------------------IRVIT 242

Query: 289 RARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKY 346
            ARSFL+HQVR MVG L  VG G  +  D+E  L+A +     PMAPA GLYL  V Y
Sbjct: 243 SARSFLHHQVRSMVGSLMRVGEGRWSADDLEAALKAADRTRCGPMAPAAGLYLAAVSY 245

BLAST of CcUC11G220670 vs. ExPASy Swiss-Prot
Match: A7HPQ3 (tRNA pseudouridine synthase A OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=truA PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.5e-45
Identity = 111/298 (37.25%), Postives = 156/298 (52.35%), Query Frame = 0

Query: 49  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC 108
           RY + +EY G+ F G Q Q   R+V  VLEE  + F GQ + +F + RTDAGVHAL  V 
Sbjct: 3   RYKLTIEYDGSPFVGWQAQTNGRSVQQVLEEGIKGFCGQELKIFGAGRTDAGVHALGQVA 62

Query: 109 HVDVERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 168
           HVD E            P  P  +  AVN  ++ H   V +V+   VP+ F AR+ A +R
Sbjct: 63  HVDFE-----------TPVAPDTLRDAVNAHMRPH--PVAIVEAEEVPDSFEARFSAVKR 122

Query: 169 TYFYRLVSGPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPI 228
            Y YR+V+   A L+ ++ +AW V +TL+  AM +A + LVG+HDF++FR+  CQA SP+
Sbjct: 123 HYMYRIVN-RRAPLTLDRGQAWLVHKTLDADAMHDAAQALVGNHDFTTFRSVQCQAKSPV 182

Query: 229 RTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTAR 288
           +T+DE+ V                             +++  E             +  R
Sbjct: 183 KTVDEISVSR---------------------------YADEIE-------------IVCR 242

Query: 289 ARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYD 347
           ARSFL++QVR  VG LK VG G  T   VE+ L AK+  +  P+AP  GLYL  V Y+
Sbjct: 243 ARSFLHNQVRSFVGTLKMVGDGSWTRRKVEKALAAKDRAACGPVAPPDGLYLLQVDYE 246

BLAST of CcUC11G220670 vs. ExPASy Swiss-Prot
Match: A9ILJ7 (tRNA pseudouridine synthase A OS=Bartonella tribocorum (strain CIP 105476 / IBS 506) OX=382640 GN=truA PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.8e-44
Identity = 107/301 (35.55%), Postives = 159/301 (52.82%), Query Frame = 0

Query: 47  IQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSN 106
           + R+ + +EY G+ ++G Q+Q    T+ G LE+A  +F GQ +++  + RTDAGVHA   
Sbjct: 1   MSRFKLTLEYDGSNYAGWQRQAKLHTIQGALEQAIFRFSGQQLTITTAGRTDAGVHATGQ 60

Query: 107 VCHVDVERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQ 166
           V HVD  +          PPH    V  A+N  L++    +++++V +VP+DF AR+ A 
Sbjct: 61  VAHVDFIK--------NWPPH---TVRDALNALLRQQSEAISILNVENVPDDFDARFSAI 120

Query: 167 ERTYFYRLVS--GPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQA 226
           +R Y +++++   P AL      R W +P+ LN  AM EA + LVG HDF++FR+A CQA
Sbjct: 121 KRHYLFKILNRRSPPAL---NAKRVWWIPKPLNAQAMHEAAQKLVGQHDFTTFRSAHCQA 180

Query: 227 NSPIRTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFV 286
            SPIRTL+ LD+                Q++G+E                          
Sbjct: 181 KSPIRTLERLDI----------------QKEGEE------------------------IF 240

Query: 287 VTARARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVK 346
           + A+ARSFL+HQ+R   G L  VG G  T  D+E  L AK+      +AP  GLYL  V+
Sbjct: 241 IYAKARSFLHHQIRSFAGSLMEVGIGRWTAQDLEAALHAKDRKRCGVVAPPSGLYLTKVE 247

BLAST of CcUC11G220670 vs. ExPASy TrEMBL
Match: A0A5D3CZZ6 (tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002730 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.4e-190
Identity = 327/350 (93.43%), Postives = 334/350 (95.43%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLE S DSSSQRPLKIGKT D+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           FSGSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEE ENE PHSET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVGTGDLTVGDVERILEAKNV+SA PMAPACGLYLGHVKYDLPPA
Sbjct: 301 VGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLPPA 350

BLAST of CcUC11G220670 vs. ExPASy TrEMBL
Match: A0A1S3BDG9 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.4e-190
Identity = 327/350 (93.43%), Postives = 334/350 (95.43%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLE S DSSSQRPLKIGKT D+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           FSGSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEE ENE PHSET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVGTGDLTVGDVERILEAKNV+SA PMAPACGLYLGHVKYDLPPA
Sbjct: 301 VGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLPPA 350

BLAST of CcUC11G220670 vs. ExPASy TrEMBL
Match: A0A1S3BDY4 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.3e-188
Identity = 326/350 (93.14%), Postives = 333/350 (95.14%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLE S DSSSQRPLKIGKT D+V  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           FSGSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEE ENE PHSET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVGTGDLTVGD ERILEAKNV+SA PMAPACGLYLGHVKYDLPPA
Sbjct: 301 VGVLKAVGTGDLTVGD-ERILEAKNVSSARPMAPACGLYLGHVKYDLPPA 349

BLAST of CcUC11G220670 vs. ExPASy TrEMBL
Match: A0A0A0KRX0 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_5G586020 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 9.6e-187
Identity = 323/350 (92.29%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKHGLE S DSSSQRPLKIGKT DE+  GDEEEAE MN  PGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           F GSQ+QP CRTVVG LEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDEL+VCEV 
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEE E EVP SET EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPA 351
           VGVLKAVG+GDLTVGDV RILEAKNV+SA PMAPACGLYLGHVKYDLPPA
Sbjct: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPA 350

BLAST of CcUC11G220670 vs. ExPASy TrEMBL
Match: A0A6J1KFZ8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492882 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.0e-185
Identity = 318/352 (90.34%), Postives = 334/352 (94.89%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTR 60
           MTENQKH LEFS DSS QRPLKIGKT +E L GD+EEAE MNRNPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHRLEFSSDSSPQRPLKIGKTTNEDLPGDKEEAESMNRNPGIQRYLVAVEYIGTR 60

Query: 61  FSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERLSKRKP 120
           FSGSQ+QP CRTVVGVLEEAF+KFIGQ VSVFCSSRTDAGVHALSNVCHVDVER+SKRKP
Sbjct: 61  FSGSQQQPNCRTVVGVLEEAFRKFIGQSVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180
           GEVLPPHEPGVV RAVNHFLQK++GDVTVVDVRSVPND+HARYKAQERTYFYRLVSGPE 
Sbjct: 121 GEVLPPHEPGVVRRAVNHFLQKNDGDVTVVDVRSVPNDYHARYKAQERTYFYRLVSGPET 180

Query: 181 LLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEVV 240
           L SF+KDR WHVPE LNIGAMQEACKVLVGHHDFSSFRAAGCQANSP+RTLDELDVCEV+
Sbjct: 181 LSSFDKDRVWHVPEILNIGAMQEACKVLVGHHDFSSFRAAGCQANSPMRTLDELDVCEVI 240

Query: 241 STPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGVRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFP+SMERKQRQGKEEGENEV HSE++EVGFG+RRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPSSMERKQRQGKEEGENEVLHSESYEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVKYDLPPASP 353
           VGVLK VGTGDLTVGDVERILEAKNV+SASPMAPACGLYLGHVKYDLPP  P
Sbjct: 301 VGVLKVVGTGDLTVGDVERILEAKNVSSASPMAPACGLYLGHVKYDLPPPLP 352

BLAST of CcUC11G220670 vs. TAIR 10
Match: AT1G09800.1 (Pseudouridine synthase family protein )

HSP 1 Score: 455.7 bits (1171), Expect = 3.4e-128
Identity = 238/374 (63.64%), Postives = 277/374 (74.06%), Query Frame = 0

Query: 1   MTENQKHGLEFSFDSSSQRPLKIGKTADEVLRGDEE--------EAEPMNR-NPGIQRYL 60
           M++N KH L+ +  +S +   ++ K A      DEE        + E MN  NP +QRYL
Sbjct: 1   MSDNLKHPLDCT--ASEESLERLSKVARIEGFSDEESEEQQGLADLESMNPVNPRVQRYL 60

Query: 61  VAVEYIGTRFSGSQKQPTCRTVVGVLEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVD 120
           VA+EYIGTRFSGSQ+Q   RTVVGVL+EAF KFIGQPV +FCSSRTDAGVHALSNVCHVD
Sbjct: 61  VAIEYIGTRFSGSQQQAKDRTVVGVLQEAFHKFIGQPVKIFCSSRTDAGVHALSNVCHVD 120

Query: 121 VERLSKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYF 180
           VER+SKRKPGEVLPPHEPGVV RAVNHFLQ+ +GDV V+DVR VP+++HARYKAQERTYF
Sbjct: 121 VERISKRKPGEVLPPHEPGVVQRAVNHFLQRKDGDVMVIDVRCVPSNYHARYKAQERTYF 180

Query: 181 YRLVSGPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTL 240
           YRL+SG + L   EKDR WHVPE LN+  MQEAC+VLVG HDFSSFRAAGCQA SP+R+L
Sbjct: 181 YRLLSGSDPLSILEKDRCWHVPEELNLRFMQEACRVLVGSHDFSSFRAAGCQAKSPMRSL 240

Query: 241 DELDVCEVVSTPYFPTSMER---KQRQGKEEGENEVPHSETFEV--------------GF 300
           DEL++ EV STPYFP+  ER       G     +  P SET  V               F
Sbjct: 241 DELNITEVPSTPYFPSITERTLSNLNNGDPLACSNQPKSETAGVTTNVGEVEGSTEGDSF 300

Query: 301 GVRRRHRCFVVTARARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAP 349
           G RRRHRC+VVTAR+R FLYHQVRL+VG LK VGTG+LTV DVERILEAK V++A PMAP
Sbjct: 301 GRRRRHRCYVVTARSRGFLYHQVRLIVGALKCVGTGELTVSDVERILEAKTVSAAKPMAP 360

BLAST of CcUC11G220670 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-10
Identity = 71/288 (24.65%), Postives = 108/288 (37.50%), Query Frame = 0

Query: 48  QRYL-VAVEYIGTRFSG----SQKQPTCRTVVGVLEEAFQKFIGQPVSVFCS--SRTDAG 107
           +RY+ + + Y G RF G    +Q +P+  + V    E  +  +G       S   RTD G
Sbjct: 89  KRYVALKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKG 148

Query: 108 VHALSNVCHVDVERLSKRKPGEVLPPHEPGVVHRAVNHFL----QKHEGDVTVVDVRSVP 167
           V +   V  + +    K  PG+           R    ++    +    D+ V+     P
Sbjct: 149 VSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAP 208

Query: 168 NDFHARYKAQERTYFYRLVSGPEALLSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSS 227
            DFHAR+    R Y Y                 W   + LN+ AM  A K  +G HDF +
Sbjct: 209 IDFHARFSCYAREYKYFF---------------WR--QNLNLSAMDFAGKKFIGEHDFRN 268

Query: 228 FRAAGCQANSPIRTLDELDVCEVVSTPYFPTSMERKQRQGKEEGENEVPHSETFEVGFGV 287
           F    C          ++DV  V       T  E    Q   EG+               
Sbjct: 269 F----C----------KMDVANVHCYTRRVTFFEVSPCQNSHEGD--------------- 326

Query: 288 RRRHRCFVVTARARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAK 325
               +    T R  +FL+HQ+R MV VL  +G G  +V  ++ +L+ K
Sbjct: 329 ----QLCTFTMRGSAFLWHQIRCMVAVLFMIGQGVESVDVIDTLLDTK 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877867.13.0e-19094.29tRNA pseudouridine synthase A isoform X2 [Benincasa hispida][more]
XP_008445970.15.1e-19093.43PREDICTED: tRNA pseudouridine synthase A isoform X1 [Cucumis melo] >KAA0034150.1... [more]
XP_008445972.12.8e-18893.14PREDICTED: tRNA pseudouridine synthase A isoform X2 [Cucumis melo][more]
XP_038877866.11.4e-18791.16tRNA pseudouridine synthase A isoform X1 [Benincasa hispida][more]
XP_011655540.12.0e-18692.29uncharacterized protein LOC101221274 isoform X1 [Cucumis sativus] >KGN51629.1 hy... [more]
Match NameE-valueIdentityDescription
Q2WAT02.5e-4639.06tRNA pseudouridine synthase A OS=Magnetospirillum magneticum (strain AMB-1 / ATC... [more]
B6IPI03.2e-4640.13tRNA pseudouridine synthase A OS=Rhodospirillum centenum (strain ATCC 51521 / SW... [more]
A7IKI91.6e-4538.59tRNA pseudouridine synthase A OS=Xanthobacter autotrophicus (strain ATCC BAA-115... [more]
A7HPQ33.5e-4537.25tRNA pseudouridine synthase A OS=Parvibaculum lavamentivorans (strain DS-1 / DSM... [more]
A9ILJ71.8e-4435.55tRNA pseudouridine synthase A OS=Bartonella tribocorum (strain CIP 105476 / IBS ... [more]
Match NameE-valueIdentityDescription
A0A5D3CZZ62.4e-19093.43tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BDG92.4e-19093.43tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1[more]
A0A1S3BDY41.3e-18893.14tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1[more]
A0A0A0KRX09.6e-18792.29tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_5G586020 PE=3 SV=1[more]
A0A6J1KFZ84.0e-18590.34tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492882 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G09800.13.4e-12863.64Pseudouridine synthase family protein [more]
AT1G34150.12.2e-1024.65Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 204..346
e-value: 2.3E-31
score: 108.5
coord: 53..163
e-value: 9.6E-8
score: 32.5
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 45..165
e-value: 1.2E-28
score: 101.4
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3D3.30.70.660coord: 167..347
e-value: 6.9E-40
score: 138.1
NoneNo IPR availablePIRSRPIRSR001430-2PIRSR001430-2coord: 87..236
e-value: 2.8E-35
score: 119.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR11142:SF0TRNA PSEUDOURIDINE SYNTHASE-LIKE 1coord: 32..346
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 32..346
IPR001406Pseudouridine synthase I, TruAHAMAPMF_00171TruAcoord: 48..345
score: 30.022173
IPR001406Pseudouridine synthase I, TruACDDcd02570PseudoU_synth_EcTruAcoord: 54..345
e-value: 1.84243E-107
score: 312.485
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 47..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC11G220670.1CcUC11G220670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031119 tRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0003723 RNA binding
molecular_function GO:0106029 tRNA pseudouridine synthase activity
molecular_function GO:0009982 pseudouridine synthase activity