CcUC10G195110 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC10G195110
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
Descriptionagamous-like MADS-box protein AGL62
LocationCicolChr10: 19971615 .. 19972728 (-)
RNA-Seq ExpressionCcUC10G195110
SyntenyCcUC10G195110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGAGCAGAGGCAGGCAAAAGGTGGAGATGGTGAAGATGCCAAATGAGAGCAACCTTCAGGTCACCTTCTCGAAGCGTCGGTCCGGTCTCTTCAAGAAAGCTAGCGAGCTCTGCACGCTGTGTGGTGCTGAGATTGCCATCATCGTCTTCTCTCCCGGGAAGAAGGTTTTCTCCTTTGGTCACCCTTGCGTTGAGGCTCTCATCGAACGCTTTGTGACACGAAACCCTCCACCGAGCTCCGGTACGCTGCAGCTAATTGAAGCCCACCGGAATGCCAATGTTCGAGAACTGAATGCACAACTTACTCAAGTGCTGAACCAGCTTGAAATGGAGCGGAAGCGCAGTGAAGAGTTGAACAAACTGCGGAAGGCAAGCCAGGTTTTGATAACTATTTTCTATTTTCCTTTTTTAATTTTTAAACTAAAACATTTTCACAATTCCAAACTTTTTTTTAAACATAACCATTTGATGTTTAAAAATTGTTTCCGTTTTCTCCCAATTTATTTATCATACTTTCATCTTTCTTAAAGTATCATTTAAATCTTTAACCAATTCTTAAAAAAAAAAAGAAAAATTAAGAAATTTTTTTTCTAAACATTTTGTAAAAAGTAGAAAACAAAAGAAGAAAATCGTAAGTATAGATGTAAACTTTATAAACCGATTATTAAAACGAAAACAAGAATTTTTAAACTCGAAATCGTTATCAAACAAAAACCCAAACTCCAAAATCTATGCAAGGTTCAAATATTCCTATTAAATTACTATTGAATGTTTAAAGTATAGGTGAATAAATAATAACATATTCAATATTTTATATATATATATTAAGGCACAATGTTGGTGGGAGCTTCCCATAGAAGAAATGGAAATGCATCAACTAGAACAACTTAAAGCATCGTTGGATGAGCTCAAGAAGAATGTCACACAACAAGCTGATCGGATTTTAATTCAGACTTCTAATGCCAATCCGCCGCCGCAGTTCTTCCCGACTCCATCAACACCGGCGAACCCTAGCCAGCAAGCTGGCCTTTTCGTCGTCGATCCGAAGAACATCATCTCCCATCTTCCCTACAACTATGGCTCCTATGGATCAAGAGGTTTCTTTTGA

mRNA sequence

ATGAGGAAGAGCAGAGGCAGGCAAAAGGTGGAGATGGTGAAGATGCCAAATGAGAGCAACCTTCAGGTCACCTTCTCGAAGCGTCGGTCCGGTCTCTTCAAGAAAGCTAGCGAGCTCTGCACGCTGTGTGGTGCTGAGATTGCCATCATCGTCTTCTCTCCCGGGAAGAAGGTTTTCTCCTTTGGTCACCCTTGCGTTGAGGCTCTCATCGAACGCTTTGTGACACGAAACCCTCCACCGAGCTCCGGTACGCTGCAGCTAATTGAAGCCCACCGGAATGCCAATGTTCGAGAACTGAATGCACAACTTACTCAAGTGCTGAACCAGCTTGAAATGGAGCGGAAGCGCAGTGAAGAGTTGAACAAACTGCGGAAGGCAAGCCAGGCACAATGTTGGTGGGAGCTTCCCATAGAAGAAATGGAAATGCATCAACTAGAACAACTTAAAGCATCGTTGGATGAGCTCAAGAAGAATGTCACACAACAAGCTGATCGGATTTTAATTCAGACTTCTAATGCCAATCCGCCGCCGCAGTTCTTCCCGACTCCATCAACACCGGCGAACCCTAGCCAGCAAGCTGGCCTTTTCGTCGTCGATCCGAAGAACATCATCTCCCATCTTCCCTACAACTATGGCTCCTATGGATCAAGAGGTTTCTTTTGA

Coding sequence (CDS)

ATGAGGAAGAGCAGAGGCAGGCAAAAGGTGGAGATGGTGAAGATGCCAAATGAGAGCAACCTTCAGGTCACCTTCTCGAAGCGTCGGTCCGGTCTCTTCAAGAAAGCTAGCGAGCTCTGCACGCTGTGTGGTGCTGAGATTGCCATCATCGTCTTCTCTCCCGGGAAGAAGGTTTTCTCCTTTGGTCACCCTTGCGTTGAGGCTCTCATCGAACGCTTTGTGACACGAAACCCTCCACCGAGCTCCGGTACGCTGCAGCTAATTGAAGCCCACCGGAATGCCAATGTTCGAGAACTGAATGCACAACTTACTCAAGTGCTGAACCAGCTTGAAATGGAGCGGAAGCGCAGTGAAGAGTTGAACAAACTGCGGAAGGCAAGCCAGGCACAATGTTGGTGGGAGCTTCCCATAGAAGAAATGGAAATGCATCAACTAGAACAACTTAAAGCATCGTTGGATGAGCTCAAGAAGAATGTCACACAACAAGCTGATCGGATTTTAATTCAGACTTCTAATGCCAATCCGCCGCCGCAGTTCTTCCCGACTCCATCAACACCGGCGAACCCTAGCCAGCAAGCTGGCCTTTTCGTCGTCGATCCGAAGAACATCATCTCCCATCTTCCCTACAACTATGGCTCCTATGGATCAAGAGGTTTCTTTTGA

Protein sequence

MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFSFGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEELNKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFFPTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF
Homology
BLAST of CcUC10G195110 vs. NCBI nr
Match: XP_022942821.1 (agamous-like MADS-box protein AGL62 [Cucurbita moschata] >XP_023516147.1 agamous-like MADS-box protein AGL62 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.5 bits (1046), Expect = 7.2e-110
Identity = 206/220 (93.64%), Postives = 212/220 (96.36%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLN LEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNHLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFF 180
           NKLRKA+Q QCWWELPIEEME+HQLEQLK SLDELKKNVTQQADRILIQTSN NPPPQFF
Sbjct: 121 NKLRKANQPQCWWELPIEEMELHQLEQLKLSLDELKKNVTQQADRILIQTSNGNPPPQFF 180

Query: 181 PTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
           PTP TPANP+QQ G++VVD KN+ISHLPYNYGSYGSRGFF
Sbjct: 181 PTP-TPANPNQQGGVYVVDQKNMISHLPYNYGSYGSRGFF 219

BLAST of CcUC10G195110 vs. NCBI nr
Match: XP_022149295.1 (agamous-like MADS-box protein AGL62 [Momordica charantia])

HSP 1 Score: 404.8 bits (1039), Expect = 4.7e-109
Identity = 205/220 (93.18%), Postives = 213/220 (96.82%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELN+QLTQVLNQLEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNSQLTQVLNQLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFF 180
           NKLRKASQAQ WWELPIEEMEM+QLEQLK SL+ELKKNVTQ ADRIL+QTSN NPPPQ+F
Sbjct: 121 NKLRKASQAQFWWELPIEEMEMNQLEQLKTSLEELKKNVTQHADRILVQTSNGNPPPQYF 180

Query: 181 PTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
           PTPS PANP+QQ G+FVVDPKN+ISHLPYNYGSYGSRGFF
Sbjct: 181 PTPS-PANPNQQGGVFVVDPKNMISHLPYNYGSYGSRGFF 219

BLAST of CcUC10G195110 vs. NCBI nr
Match: KAG7030923.1 (Agamous-like MADS-box protein AGL62, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 404.4 bits (1038), Expect = 6.1e-109
Identity = 205/220 (93.18%), Postives = 211/220 (95.91%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLN LEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNHLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFF 180
           NKLRKA+Q QCWWELPIEEME+HQLEQLK SLDELKKNVTQQADRILIQTSN NPPPQFF
Sbjct: 121 NKLRKANQPQCWWELPIEEMELHQLEQLKLSLDELKKNVTQQADRILIQTSNGNPPPQFF 180

Query: 181 PTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
            TP TPANP+QQ G++VVD KN+ISHLPYNYGSYGSRGFF
Sbjct: 181 STP-TPANPNQQGGVYVVDQKNMISHLPYNYGSYGSRGFF 219

BLAST of CcUC10G195110 vs. NCBI nr
Match: XP_022975753.1 (agamous-like MADS-box protein AGL62 [Cucurbita maxima])

HSP 1 Score: 402.9 bits (1034), Expect = 1.8e-108
Identity = 204/220 (92.73%), Postives = 211/220 (95.91%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLN LEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNHLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFF 180
           NKLRKA+Q QCWWELPIEEME++QLEQLK SLDELKKNV QQADRILIQTSN NPPPQFF
Sbjct: 121 NKLRKANQPQCWWELPIEEMELYQLEQLKLSLDELKKNVMQQADRILIQTSNGNPPPQFF 180

Query: 181 PTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
           PTP TPANP+QQ G++VVD KN+ISHLPYNYGSYGSRGFF
Sbjct: 181 PTP-TPANPNQQGGVYVVDQKNMISHLPYNYGSYGSRGFF 219

BLAST of CcUC10G195110 vs. NCBI nr
Match: XP_008454428.1 (PREDICTED: agamous-like MADS-box protein AGL62 [Cucumis melo] >KAA0044310.1 agamous-like MADS-box protein AGL62 [Cucumis melo var. makuwa])

HSP 1 Score: 400.6 bits (1028), Expect = 8.8e-108
Identity = 209/226 (92.48%), Postives = 212/226 (93.81%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQT-SNANPPPQF 180
           NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQT SNANPP Q 
Sbjct: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSSNANPPTQL 180

Query: 181 -----FPTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
                 PTP+T   P+QQ GLFVVDPKNIISHLP+NYGSYGSRGFF
Sbjct: 181 IFPTQIPTPTTTNGPNQQPGLFVVDPKNIISHLPFNYGSYGSRGFF 226

BLAST of CcUC10G195110 vs. ExPASy Swiss-Prot
Match: Q9FKK2 (Agamous-like MADS-box protein AGL62 OS=Arabidopsis thaliana OX=3702 GN=AGL62 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-49
Identity = 109/190 (57.37%), Postives = 141/190 (74.21%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           ++KS+GRQK+EMVKM NESNLQVTFSKRRSGLFKKASELCTLCGAE+AI+VFSPG+KVFS
Sbjct: 2   VKKSKGRQKIEMVKMKNESNLQVTFSKRRSGLFKKASELCTLCGAEVAIVVFSPGRKVFS 61

Query: 61  FGHPCVEALIERFVTRN--PPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSE 120
           FGHP V+++I+RF+  N  PP     +QL E  RN+ V++LN  LTQVL+QLE E+K+ +
Sbjct: 62  FGHPNVDSVIDRFINNNPLPPHQHNNMQLRETRRNSIVQDLNNHLTQVLSQLETEKKKYD 121

Query: 121 ELNKLRKASQA-QCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPP 180
           EL K+R+ ++A   WWE P+EE+ + QLE  K +L+ LKK VT +A R       AN P 
Sbjct: 122 ELKKIREKTKALGNWWEDPVEELALSQLEGFKGNLENLKKVVTVEASRFF----QANVPN 181

Query: 181 QFFPTPSTPA 188
            +  + S  A
Sbjct: 182 FYVGSSSNNA 187

BLAST of CcUC10G195110 vs. ExPASy Swiss-Prot
Match: Q4PSU4 (Agamous-like MADS-box protein AGL61 OS=Arabidopsis thaliana OX=3702 GN=AGL61 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.6e-38
Identity = 94/197 (47.72%), Postives = 130/197 (65.99%), Query Frame = 0

Query: 2   RKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFSF 61
           ++S GRQK+ MVK+  ES+ QVTFSKRR+GLFKKASELCTLCGAEI IIVFSP KK FSF
Sbjct: 59  KESIGRQKIPMVKIKKESHRQVTFSKRRAGLFKKASELCTLCGAEIGIIVFSPAKKPFSF 118

Query: 62  GHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEELN 121
           GHP VE++++R+V+RN    + + QL      A   ELN QLT +L+++E E+K+ + + 
Sbjct: 119 GHPSVESVLDRYVSRNNMSLAQSQQL--QGSPAASCELNMQLTHILSEVEEEKKKGQAME 178

Query: 122 KLRKASQAQC---WWELPIEEMEMHQLEQLKASLDELKKNV-------TQQADRILIQTS 181
           ++RK S  +    WWE P+EEM M QL+++K +L+EL+K V        +  D +     
Sbjct: 179 EMRKESVRRSMINWWEKPVEEMNMVQLQEMKYALEELRKTVVTNMASFNEAKDDVFGFLD 238

Query: 182 NANPPPQFFPTPSTPAN 189
           N    P +   PS P+N
Sbjct: 239 NKVTVPPYVNMPSGPSN 253

BLAST of CcUC10G195110 vs. ExPASy Swiss-Prot
Match: Q9LMM8 (Agamous-like MADS-box protein AGL28 OS=Arabidopsis thaliana OX=3702 GN=AGL28 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.8e-29
Identity = 74/166 (44.58%), Postives = 102/166 (61.45%), Query Frame = 0

Query: 2   RKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFSF 61
           RK+ GR+K+E+VKM NESNLQVTFSKRRSGLFKK SELCTLC AEIAIIVFSP  K +SF
Sbjct: 3   RKNLGRRKIELVKMTNESNLQVTFSKRRSGLFKKGSELCTLCDAEIAIIVFSPSGKAYSF 62

Query: 62  GHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL- 121
           GHP V  L++  + R            E+     ++ LN  LT+V+ + E E++  + + 
Sbjct: 63  GHPNVNKLLDHSLGR--VIRHNNTNFAESRTKLRIQMLNESLTEVMAEKEKEQETKQSIV 122

Query: 122 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRI 167
              R+   A+ WW     E+ + Q   +K  L+ LKK V ++  ++
Sbjct: 123 QNERENKDAEKWWRNSPTELNLAQSTSMKCDLEALKKEVDEKVAQL 166

BLAST of CcUC10G195110 vs. ExPASy Swiss-Prot
Match: O80807 (Agamous-like MADS-box protein AGL23 OS=Arabidopsis thaliana OX=3702 GN=AGL23 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.2e-28
Identity = 81/195 (41.54%), Postives = 118/195 (60.51%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           ++K+ GR+KVE+VKM  ESNLQVTFSKR++GLFKKASE CTLC A+IA+IVFSP  KVFS
Sbjct: 2   VKKTLGRRKVEIVKMTKESNLQVTFSKRKAGLFKKASEFCTLCDAKIAMIVFSPAGKVFS 61

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERK-RSEE 120
           FGHP V+ L++ F  R          L E++   +V+ LN   T+V  ++E E+K +   
Sbjct: 62  FGHPNVDVLLDHF--RGCVVGHNNTNLDESYTKLHVQMLNKSYTEVKAEVEKEQKNKQSR 121

Query: 121 LNKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQF 180
               R+   A+ WW     E+ + Q   +   L +LKK V ++A +++ QT+    P  +
Sbjct: 122 AQNERENENAEEWWSKSPLELNLSQSTCMIRVLKDLKKIVDEKAIQLIHQTN----PNFY 181

Query: 181 FPTPSTPANPSQQAG 195
             + S  A P+  +G
Sbjct: 182 VGSSSNAAAPATVSG 190

BLAST of CcUC10G195110 vs. ExPASy Swiss-Prot
Match: O64703 (Agamous-like MADS-box protein AGL29 OS=Arabidopsis thaliana OX=3702 GN=AGL29 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-17
Identity = 58/162 (35.80%), Postives = 96/162 (59.26%), Query Frame = 0

Query: 6   GRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFSFGHPC 65
           GR+K++M  + + +  QVTFSKRR+GLFKKASEL TLC AE+ I+VFSPG K FS+G P 
Sbjct: 2   GRRKIKMEMVQDMNTRQVTFSKRRTGLFKKASELATLCNAELGIVVFSPGGKPFSYGKPN 61

Query: 66  VEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEE-LNKLR 125
           ++++ ERF+       SG  +    +R   ++ L+ +L  +  ++E E++R E+   KL 
Sbjct: 62  LDSVAERFMREYDDSDSGDEEKSGNYR-PKLKRLSERLDLLNQEVEAEKERGEKSQEKLE 121

Query: 126 KASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRI 167
            A   +  ++  IE + + +L + K  L  +   +  Q + +
Sbjct: 122 SAGDER--FKESIETLTLDELNEYKDRLQTVHGRIEGQVNHL 160

BLAST of CcUC10G195110 vs. ExPASy TrEMBL
Match: A0A6J1FPY5 (agamous-like MADS-box protein AGL62 OS=Cucurbita moschata OX=3662 GN=LOC111447736 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.5e-110
Identity = 206/220 (93.64%), Postives = 212/220 (96.36%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLN LEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNHLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFF 180
           NKLRKA+Q QCWWELPIEEME+HQLEQLK SLDELKKNVTQQADRILIQTSN NPPPQFF
Sbjct: 121 NKLRKANQPQCWWELPIEEMELHQLEQLKLSLDELKKNVTQQADRILIQTSNGNPPPQFF 180

Query: 181 PTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
           PTP TPANP+QQ G++VVD KN+ISHLPYNYGSYGSRGFF
Sbjct: 181 PTP-TPANPNQQGGVYVVDQKNMISHLPYNYGSYGSRGFF 219

BLAST of CcUC10G195110 vs. ExPASy TrEMBL
Match: A0A6J1D5B6 (agamous-like MADS-box protein AGL62 OS=Momordica charantia OX=3673 GN=LOC111017745 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.3e-109
Identity = 205/220 (93.18%), Postives = 213/220 (96.82%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELN+QLTQVLNQLEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNSQLTQVLNQLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFF 180
           NKLRKASQAQ WWELPIEEMEM+QLEQLK SL+ELKKNVTQ ADRIL+QTSN NPPPQ+F
Sbjct: 121 NKLRKASQAQFWWELPIEEMEMNQLEQLKTSLEELKKNVTQHADRILVQTSNGNPPPQYF 180

Query: 181 PTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
           PTPS PANP+QQ G+FVVDPKN+ISHLPYNYGSYGSRGFF
Sbjct: 181 PTPS-PANPNQQGGVFVVDPKNMISHLPYNYGSYGSRGFF 219

BLAST of CcUC10G195110 vs. ExPASy TrEMBL
Match: A0A6J1IK72 (agamous-like MADS-box protein AGL62 OS=Cucurbita maxima OX=3661 GN=LOC111476097 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 8.6e-109
Identity = 204/220 (92.73%), Postives = 211/220 (95.91%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLN LEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNHLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQFF 180
           NKLRKA+Q QCWWELPIEEME++QLEQLK SLDELKKNV QQADRILIQTSN NPPPQFF
Sbjct: 121 NKLRKANQPQCWWELPIEEMELYQLEQLKLSLDELKKNVMQQADRILIQTSNGNPPPQFF 180

Query: 181 PTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
           PTP TPANP+QQ G++VVD KN+ISHLPYNYGSYGSRGFF
Sbjct: 181 PTP-TPANPNQQGGVYVVDQKNMISHLPYNYGSYGSRGFF 219

BLAST of CcUC10G195110 vs. ExPASy TrEMBL
Match: A0A5A7TM75 (Agamous-like MADS-box protein AGL62 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00310 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.3e-108
Identity = 209/226 (92.48%), Postives = 212/226 (93.81%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQT-SNANPPPQF 180
           NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQT SNANPP Q 
Sbjct: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSSNANPPTQL 180

Query: 181 -----FPTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
                 PTP+T   P+QQ GLFVVDPKNIISHLP+NYGSYGSRGFF
Sbjct: 181 IFPTQIPTPTTTNGPNQQPGLFVVDPKNIISHLPFNYGSYGSRGFF 226

BLAST of CcUC10G195110 vs. ExPASy TrEMBL
Match: A0A1S3BYP6 (agamous-like MADS-box protein AGL62 OS=Cucumis melo OX=3656 GN=LOC103494837 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.3e-108
Identity = 209/226 (92.48%), Postives = 212/226 (93.81%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS
Sbjct: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL 120
           FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKR EEL
Sbjct: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRGEEL 120

Query: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQT-SNANPPPQF 180
           NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQT SNANPP Q 
Sbjct: 121 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSSNANPPTQL 180

Query: 181 -----FPTPSTPANPSQQAGLFVVDPKNIISHLPYNYGSYGSRGFF 221
                 PTP+T   P+QQ GLFVVDPKNIISHLP+NYGSYGSRGFF
Sbjct: 181 IFPTQIPTPTTTNGPNQQPGLFVVDPKNIISHLPFNYGSYGSRGFF 226

BLAST of CcUC10G195110 vs. TAIR 10
Match: AT5G60440.1 (AGAMOUS-like 62 )

HSP 1 Score: 198.0 bits (502), Expect = 8.1e-51
Identity = 109/190 (57.37%), Postives = 141/190 (74.21%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           ++KS+GRQK+EMVKM NESNLQVTFSKRRSGLFKKASELCTLCGAE+AI+VFSPG+KVFS
Sbjct: 2   VKKSKGRQKIEMVKMKNESNLQVTFSKRRSGLFKKASELCTLCGAEVAIVVFSPGRKVFS 61

Query: 61  FGHPCVEALIERFVTRN--PPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSE 120
           FGHP V+++I+RF+  N  PP     +QL E  RN+ V++LN  LTQVL+QLE E+K+ +
Sbjct: 62  FGHPNVDSVIDRFINNNPLPPHQHNNMQLRETRRNSIVQDLNNHLTQVLSQLETEKKKYD 121

Query: 121 ELNKLRKASQA-QCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPP 180
           EL K+R+ ++A   WWE P+EE+ + QLE  K +L+ LKK VT +A R       AN P 
Sbjct: 122 ELKKIREKTKALGNWWEDPVEELALSQLEGFKGNLENLKKVVTVEASRFF----QANVPN 181

Query: 181 QFFPTPSTPA 188
            +  + S  A
Sbjct: 182 FYVGSSSNNA 187

BLAST of CcUC10G195110 vs. TAIR 10
Match: AT2G24840.1 (AGAMOUS-like 61 )

HSP 1 Score: 160.2 bits (404), Expect = 1.9e-39
Identity = 94/197 (47.72%), Postives = 130/197 (65.99%), Query Frame = 0

Query: 2   RKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFSF 61
           ++S GRQK+ MVK+  ES+ QVTFSKRR+GLFKKASELCTLCGAEI IIVFSP KK FSF
Sbjct: 59  KESIGRQKIPMVKIKKESHRQVTFSKRRAGLFKKASELCTLCGAEIGIIVFSPAKKPFSF 118

Query: 62  GHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEELN 121
           GHP VE++++R+V+RN    + + QL      A   ELN QLT +L+++E E+K+ + + 
Sbjct: 119 GHPSVESVLDRYVSRNNMSLAQSQQL--QGSPAASCELNMQLTHILSEVEEEKKKGQAME 178

Query: 122 KLRKASQAQC---WWELPIEEMEMHQLEQLKASLDELKKNV-------TQQADRILIQTS 181
           ++RK S  +    WWE P+EEM M QL+++K +L+EL+K V        +  D +     
Sbjct: 179 EMRKESVRRSMINWWEKPVEEMNMVQLQEMKYALEELRKTVVTNMASFNEAKDDVFGFLD 238

Query: 182 NANPPPQFFPTPSTPAN 189
           N    P +   PS P+N
Sbjct: 239 NKVTVPPYVNMPSGPSN 253

BLAST of CcUC10G195110 vs. TAIR 10
Match: AT4G36590.1 (MADS-box transcription factor family protein )

HSP 1 Score: 146.4 bits (368), Expect = 2.8e-35
Identity = 86/186 (46.24%), Postives = 123/186 (66.13%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           +R ++GRQK+EM KM NESNLQVTFSKRR GLFKKASELCTL GAEI +IVFSPG KVFS
Sbjct: 2   VRSTKGRQKIEMKKMENESNLQVTFSKRRFGLFKKASELCTLSGAEILLIVFSPGGKVFS 61

Query: 61  FGHPCVEALIERFVTRNPPPS-----SGTLQLIEAHRNANVRELNAQLTQVLNQLEMERK 120
           FGHP V+ LI RF   N   +     +  LQL+E   + N++ LN  LT+VL   E E++
Sbjct: 62  FGHPSVQELIHRFSNPNHNSAIVHHQNNNLQLVETRPDRNIQYLNNILTEVLANQEKEKQ 121

Query: 121 RSEELNKLRKA-SQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNAN 180
           +   L+ L+++  Q   W+E  +++++M++  QL ++L ++KK + ++    + Q S  N
Sbjct: 122 KRMVLDLLKESREQVGNWYEKDVKDLDMNETNQLISALQDVKKKLVRE----MSQYSQVN 181

BLAST of CcUC10G195110 vs. TAIR 10
Match: AT1G01530.1 (AGAMOUS-like 28 )

HSP 1 Score: 129.8 bits (325), Expect = 2.7e-30
Identity = 74/166 (44.58%), Postives = 102/166 (61.45%), Query Frame = 0

Query: 2   RKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFSF 61
           RK+ GR+K+E+VKM NESNLQVTFSKRRSGLFKK SELCTLC AEIAIIVFSP  K +SF
Sbjct: 3   RKNLGRRKIELVKMTNESNLQVTFSKRRSGLFKKGSELCTLCDAEIAIIVFSPSGKAYSF 62

Query: 62  GHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERKRSEEL- 121
           GHP V  L++  + R            E+     ++ LN  LT+V+ + E E++  + + 
Sbjct: 63  GHPNVNKLLDHSLGR--VIRHNNTNFAESRTKLRIQMLNESLTEVMAEKEKEQETKQSIV 122

Query: 122 NKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRI 167
              R+   A+ WW     E+ + Q   +K  L+ LKK V ++  ++
Sbjct: 123 QNERENKDAEKWWRNSPTELNLAQSTSMKCDLEALKKEVDEKVAQL 166

BLAST of CcUC10G195110 vs. TAIR 10
Match: AT1G65360.1 (AGAMOUS-like 23 )

HSP 1 Score: 126.7 bits (317), Expect = 2.3e-29
Identity = 81/195 (41.54%), Postives = 118/195 (60.51%), Query Frame = 0

Query: 1   MRKSRGRQKVEMVKMPNESNLQVTFSKRRSGLFKKASELCTLCGAEIAIIVFSPGKKVFS 60
           ++K+ GR+KVE+VKM  ESNLQVTFSKR++GLFKKASE CTLC A+IA+IVFSP  KVFS
Sbjct: 2   VKKTLGRRKVEIVKMTKESNLQVTFSKRKAGLFKKASEFCTLCDAKIAMIVFSPAGKVFS 61

Query: 61  FGHPCVEALIERFVTRNPPPSSGTLQLIEAHRNANVRELNAQLTQVLNQLEMERK-RSEE 120
           FGHP V+ L++ F  R          L E++   +V+ LN   T+V  ++E E+K +   
Sbjct: 62  FGHPNVDVLLDHF--RGCVVGHNNTNLDESYTKLHVQMLNKSYTEVKAEVEKEQKNKQSR 121

Query: 121 LNKLRKASQAQCWWELPIEEMEMHQLEQLKASLDELKKNVTQQADRILIQTSNANPPPQF 180
               R+   A+ WW     E+ + Q   +   L +LKK V ++A +++ QT+    P  +
Sbjct: 122 AQNERENENAEEWWSKSPLELNLSQSTCMIRVLKDLKKIVDEKAIQLIHQTN----PNFY 181

Query: 181 FPTPSTPANPSQQAG 195
             + S  A P+  +G
Sbjct: 182 VGSSSNAAAPATVSG 190

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022942821.17.2e-11093.64agamous-like MADS-box protein AGL62 [Cucurbita moschata] >XP_023516147.1 agamous... [more]
XP_022149295.14.7e-10993.18agamous-like MADS-box protein AGL62 [Momordica charantia][more]
KAG7030923.16.1e-10993.18Agamous-like MADS-box protein AGL62, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022975753.11.8e-10892.73agamous-like MADS-box protein AGL62 [Cucurbita maxima][more]
XP_008454428.18.8e-10892.48PREDICTED: agamous-like MADS-box protein AGL62 [Cucumis melo] >KAA0044310.1 agam... [more]
Match NameE-valueIdentityDescription
Q9FKK21.1e-4957.37Agamous-like MADS-box protein AGL62 OS=Arabidopsis thaliana OX=3702 GN=AGL62 PE=... [more]
Q4PSU42.6e-3847.72Agamous-like MADS-box protein AGL61 OS=Arabidopsis thaliana OX=3702 GN=AGL61 PE=... [more]
Q9LMM83.8e-2944.58Agamous-like MADS-box protein AGL28 OS=Arabidopsis thaliana OX=3702 GN=AGL28 PE=... [more]
O808073.2e-2841.54Agamous-like MADS-box protein AGL23 OS=Arabidopsis thaliana OX=3702 GN=AGL23 PE=... [more]
O647031.1e-1735.80Agamous-like MADS-box protein AGL29 OS=Arabidopsis thaliana OX=3702 GN=AGL29 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FPY53.5e-11093.64agamous-like MADS-box protein AGL62 OS=Cucurbita moschata OX=3662 GN=LOC11144773... [more]
A0A6J1D5B62.3e-10993.18agamous-like MADS-box protein AGL62 OS=Momordica charantia OX=3673 GN=LOC1110177... [more]
A0A6J1IK728.6e-10992.73agamous-like MADS-box protein AGL62 OS=Cucurbita maxima OX=3661 GN=LOC111476097 ... [more]
A0A5A7TM754.3e-10892.48Agamous-like MADS-box protein AGL62 OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A1S3BYP64.3e-10892.48agamous-like MADS-box protein AGL62 OS=Cucumis melo OX=3656 GN=LOC103494837 PE=4... [more]
Match NameE-valueIdentityDescription
AT5G60440.18.1e-5157.37AGAMOUS-like 62 [more]
AT2G24840.11.9e-3947.72AGAMOUS-like 61 [more]
AT4G36590.12.8e-3546.24MADS-box transcription factor family protein [more]
AT1G01530.12.7e-3044.58AGAMOUS-like 28 [more]
AT1G65360.12.3e-2941.54AGAMOUS-like 23 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 93..130
NoneNo IPR availableCOILSCoilCoilcoord: 138..165
NoneNo IPR availableGENE3D6.10.140.920coord: 83..175
e-value: 1.3E-19
score: 72.5
NoneNo IPR availablePANTHERPTHR11945:SF677AGAMOUS-LIKE MADS-BOX PROTEIN AGL62coord: 5..183
NoneNo IPR availablePANTHERPTHR11945MADS BOX PROTEINcoord: 5..183
IPR002100Transcription factor, MADS-boxPRINTSPR00404MADSDOMAINcoord: 27..42
score: 67.1
coord: 7..27
score: 46.13
coord: 42..63
score: 41.44
IPR002100Transcription factor, MADS-boxSMARTSM00432madsneu2coord: 5..64
e-value: 1.7E-30
score: 117.3
IPR002100Transcription factor, MADS-boxPFAMPF00319SRF-TFcoord: 16..61
e-value: 3.5E-24
score: 84.1
IPR002100Transcription factor, MADS-boxPROSITEPS50066MADS_BOX_2coord: 5..65
score: 23.548571
IPR036879Transcription factor, MADS-box superfamilyGENE3D3.40.1810.10coord: 17..81
e-value: 3.1E-27
score: 96.2
IPR036879Transcription factor, MADS-box superfamilySUPERFAMILY55455SRF-likecoord: 6..78
IPR033896MADS MEF2-likeCDDcd00265MADS_MEF2_likecoord: 6..73
e-value: 9.93813E-35
score: 116.498

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC10G195110.1CcUC10G195110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045944 positive regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding