CcUC10G191540 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC10G191540
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	Gibberellin 2-beta-dioxygenase 8-like
Location	CicolChr10: 7007779 .. 7009845 (-)
RNA-Seq Expression	CcUC10G191540
Synteny	CcUC10G191540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAATGGAAGGAGTGGTGAAGAAGTTGGGGAGATTAGCAGAGAAGATATCAGAGATATTAGGGCAAAGTTTGGGAATAAAATCAAGTTACTTCAAGGAAAGGTGCGAAAAAGGAAAGAGCAGCTTTCGACTGAATAGATATCCACCTTGTCCATTTGCTTCTAAAGTCTATGGTCTTATTCCTCACACCGACAGTGATTATCTCACAATTCTTTATCAACCTCAAATCAGTGGTTTGCAACTCTTGCGAGCTGGAAAATGGTTCCCTGTTAAACCTAATCCACAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTACCTACACAAACACTTTTTAAGTTTTTTTCTTCTTCATTTTGGCTCCAGTATACTCAAAACAAAAGGTTTTCAAAATGTTGAGAAATTTACAAATATGTTAATATATAACACAAGTAAATGTATCTTTGGCTTTTCAGTATACTGGTAGTTTAGGCTCTGTTTGATAACGGTTTCATTTTTAGTTTTATTCTTTTCAAATTTATGTGTTTTTCATCCAATGTATGTACTTATAGTTTTATTTTCACTAATTTTAAAGAAACACTTGAATTTCTAGTCAAAGTAAATAAAAAGGTATAGAAATTTATGGGTGTGGAAGTAATGTTTATACGTTTTATTTTCAAAAACTGAAAATAAATAACAAAATAGTTATCTATGAGTAGTTTTCAAAACTTACTCGGATTTTTTAAAACATTTCTTTAAAAAGTAGATAATAAGACAAAGATTGAAATCAATAGCTATAGAATAAGCTTAATTTTTAAAACCTAAAAACTAAAAAGAAAATTTAAAAGTTATTTTTAAAATAATTTTTTTTAGAACTAAAATTGAAAAGTTTGGAAAAAAAATGTAGATTTTGAGGGTAAGACATCAAAATATTTATTTAAAAATGTGAAAATAAAGTAAACAACTTGTTGGTAATAAAGTTTAATTTTTAAACCCAAAATCAGTACCAACATGGGCATATTTTATAAAAGTTTGCTTTTCCTTTAAAAATACTATCAAGATTTTAATGGTAAACAAAAAATATTGTTGATAAAGTAGGAAAAGAAACTTTGGATAGATTGGACAATGATTTAATATCTTTTGAATTTTCAGTTTCTATTAAACTGTCTTTGTTTTATTGCTTAACTTAAGTCACTGCTAAAACGTATATCTATCAAATCTTTTCTACCTGGTAGAGAATTATGTATACTTGGAAATGGAACGATTTAAACTTCATATATATATATATATATATATATATATATAAAACTATCATTGACGTAGTGATAAATAGGGGGTCACGTATCTATAATTTAATATTCTACGAGTTTTCTTAACTTTCAAATGTTGTAGGGTTAGACAAATTGTCCTATGAAGTTAGTCGAAGTGCACGTAAACTAACTCAAGTACTCAAAGATATAAAAAAGAAGACTTTATATATACCCTAAAGAATGAGCCAAATTGTAATCTTAAAGATTGCTTCTTCTCAAGAGTAGTATATAGGAAGAGATCAAAAGGGCATGCAAAGAGAACATTAACATATATTATATATGTATTACCAACCTTTTGTGAAGAGAGTGATCTTAATTGGTAAAAATAATATGATGGCAGGTTGTGAGCAATGATGTTTTTAGGAGCCTTAAACACAGAGTGGTTGCTTCTGAAGCAGTTGAAAGATTTTCCTTTGCATATTTCTATTGCCCATCTGATGATGTGTTGATTGAGAGTTGGTTGAAACCAAGCAATTACAGGCAATTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGCCTTTCAAGGTTTTTCTTGCACAACATGTTGCTGCCTCATTTGGGTCAATAAGTAAAGTTTTTAGTGTCACATTATTAATCAATTATAGCTACTCTACCTAACTATATGAGAAGAAAATAATTAATTAATTATTAAAAACTAGTAGGAGACTATTTGAGTGTGTTTTTGTATCCAATATTTCAACTTATCATTTACTTCTATATTATAAATATATTATTGTCTAC

mRNA sequence

AACAATGGAAGGAGTGGTGAAGAAGTTGGGGAGATTAGCAGAGAAGATATCAGAGATATTAGGGCAAAGTTTGGGAATAAAATCAAGTTACTTCAAGGAAAGGTGCGAAAAAGGAAAGAGCAGCTTTCGACTGAATAGATATCCACCTTGTCCATTTGCTTCTAAAGTCTATGGTCTTATTCCTCACACCGACAGTGATTATCTCACAATTCTTTATCAACCTCAAATCAGTGGTTTGCAACTCTTGCGAGCTGGAAAATGGTTCCCTGTTAAACCTAATCCACAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTTGTGAGCAATGATGTTTTTAGGAGCCTTAAACACAGAGTGGTTGCTTCTGAAGCAGTTGAAAGATTTTCCTTTGCATATTTCTATTGCCCATCTGATGATGTGTTGATTGAGAGTTGGTTGAAACCAAGCAATTACAGGCAATTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGCCTTTCAAGGTTTTTCTTGCACAACATGTTGCTGCCTCATTTGGGTCAATAAGTAAAGTTTTTAGTGTCACATTATTAATCAATTATAGCTACTCTACCTAACTATATGAGAAGAAAATAATTAATTAATTATTAAAAACTAGTAGGAGACTATTTGAGTGTGTTTTTGTATCCAATATTTCAACTTATCATTTACTTCTATATTATAAATATATTATTGTCTAC

Coding sequence (CDS)

ATGGAAGGAGTGGTGAAGAAGTTGGGGAGATTAGCAGAGAAGATATCAGAGATATTAGGGCAAAGTTTGGGAATAAAATCAAGTTACTTCAAGGAAAGGTGCGAAAAAGGAAAGAGCAGCTTTCGACTGAATAGATATCCACCTTGTCCATTTGCTTCTAAAGTCTATGGTCTTATTCCTCACACCGACAGTGATTATCTCACAATTCTTTATCAACCTCAAATCAGTGGTTTGCAACTCTTGCGAGCTGGAAAATGGTTCCCTGTTAAACCTAATCCACAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTTGTGAGCAATGATGTTTTTAGGAGCCTTAAACACAGAGTGGTTGCTTCTGAAGCAGTTGAAAGATTTTCCTTTGCATATTTCTATTGCCCATCTGATGATGTGTTGATTGAGAGTTGGTTGAAACCAAGCAATTACAGGCAATTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGCCTTTCAAGGTTTTTCTTGCACAACATGTTGCTGCCTCATTTGGGTCAATAA

Protein sequence

MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVVASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNMLLPHLGQ

Homology

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BLAST of CcUC10G191540 vs. NCBI nr
Match: XP_038906005.1 (gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida])

HSP 1 Score: 372.1 bits (954), Expect = 2.9e-99
Identity = 180/191 (94.24%), Postives = 185/191 (96.86%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKK GRLAE ISE+LGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP
Sbjct: 162 MEGVVKKFGRLAENISEVLGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 221

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQPQ SGLQL++AGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV
Sbjct: 222 HTDSDYLTILYQPQTSGLQLMKAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 281

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VERFSFAYFYCPSDDV+IESWLKPS YRQFSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 282 ASEGVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 341

Query: 181 LHNMLLPHLGQ 192
           LHN +LPHLGQ
Sbjct: 342 LHN-ILPHLGQ 351

[Close]

BLAST of CcUC10G191540 vs. NCBI nr
Match: TYK04745.1 (gibberellin 2-beta-dioxygenase 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 167/188 (88.83%), Postives = 181/188 (96.28%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKLG LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 1   MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 60

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQP ISGLQL+++ KWFPVKPNPQALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 61  HTDTDYLTILYQPHISGLQLMKSAKWFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVV 120

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           AS  VER+SFAYFYCPSDDV+IESWLKPS Y+QFSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 121 ASVGVERYSFAYFYCPSDDVMIESWLKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180

Query: 181 LHNMLLPH 189
           LHN++LPH
Sbjct: 181 LHNIILPH 188

[Close]

BLAST of CcUC10G191540 vs. NCBI nr
Match: XP_004152933.2 (gibberellin 2-beta-dioxygenase 8 [Cucumis sativus] >XP_031737838.1 gibberellin 2-beta-dioxygenase 8 [Cucumis sativus] >KAE8650147.1 hypothetical protein Csa_009716 [Cucumis sativus])

HSP 1 Score: 354.4 bits (908), Expect = 6.3e-94
Identity = 167/188 (88.83%), Postives = 180/188 (95.74%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKLG LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 157 MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 216

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQPQISGLQL ++ KWFPVKPNP+ALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 217 HTDTDYLTILYQPQISGLQLKKSAKWFPVKPNPRALLLNIGDLFQVVSNDIFRSLKHRVV 276

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VER+SFAYFYCPSDDV+IESWLKPS Y+ FSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 277 ASEGVERYSFAYFYCPSDDVIIESWLKPSIYKHFSYKEYRQQIEKDVEKTGNKVGLSRFF 336

Query: 181 LHNMLLPH 189
           LHN+ LPH
Sbjct: 337 LHNIALPH 344

[Close]

BLAST of CcUC10G191540 vs. NCBI nr
Match: XP_022985245.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima])

HSP 1 Score: 345.9 bits (886), Expect = 2.2e-91
Identity = 169/191 (88.48%), Postives = 178/191 (93.19%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME VVKK GRLAE I+EILGQSLGIKSSYFKERCEKG+SSFRLNRYPPCPFAS+VYGLIP
Sbjct: 160 MEAVVKKFGRLAESIAEILGQSLGIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIP 219

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQ Q+SGLQL++ GKWF VKPNP+ALLVNIGDLFQVVSND FRSLKHRVV
Sbjct: 220 HTDSDYLTILYQAQVSGLQLMKDGKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVV 279

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASEAVERFSFAYFY PSDDV+IES+ KPS YRQFSYKEYRQQIEKDVEKTGNKVGL RF 
Sbjct: 280 ASEAVERFSFAYFYSPSDDVMIESYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFL 339

Query: 181 LHNMLLPHLGQ 192
           LHNM LPHLGQ
Sbjct: 340 LHNM-LPHLGQ 349

[Close]

BLAST of CcUC10G191540 vs. NCBI nr
Match: XP_023520421.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 344.4 bits (882), Expect = 6.5e-91
Identity = 168/191 (87.96%), Postives = 178/191 (93.19%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME VVKK GRLAE I+EILGQSLGIK+SYFKERCEKG+SSFRLNRYPPCPFAS+VYGLIP
Sbjct: 144 MEAVVKKFGRLAESIAEILGQSLGIKASYFKERCEKGRSSFRLNRYPPCPFASQVYGLIP 203

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQ Q+SGLQL++ GKWF VKPNP+ALLVNIGDLFQVVSND FRSLKHRVV
Sbjct: 204 HTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVV 263

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASEAVERFSFAYFY PSDDV+IES  KPS YRQFSY+EYRQQIEKDVEKTGNKVGL RFF
Sbjct: 264 ASEAVERFSFAYFYSPSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFF 323

Query: 181 LHNMLLPHLGQ 192
           LHNM LPHLGQ
Sbjct: 324 LHNM-LPHLGQ 333

[Close]

BLAST of CcUC10G191540 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 3.3e-53
Identity = 97/182 (53.30%), Postives = 130/182 (71.43%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME    +   LA  ++E+L +  G  SS+FKE C +     R+NRYPPCP  S+VYGL+P
Sbjct: 155 MEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMP 214

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSD+LTILYQ Q+ GLQL++  +W  VKPNP+AL++NIGDLFQ  SN +++S++HRV+
Sbjct: 215 HTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVM 274

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
            +  VERFS AYF CPS D +IE       YR FS++E+RQQ+++DV+K G KVGL RF 
Sbjct: 275 TNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

Query: 181 LH 183
            H
Sbjct: 335 NH 336

[Close]

BLAST of CcUC10G191540 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.9e-46
Identity = 87/181 (48.07%), Postives = 128/181 (70.72%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           +E  V+++ R+A+ I EILG+ + + S YF+   E   S  RLN+Y P  F S+V+GL+P
Sbjct: 156 VETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVP 215

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+ +LTIL Q QI GL+L   G+W  VKP  +AL VNIGD+FQ +SN V++S++HRV+
Sbjct: 216 HTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVI 275

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           +   +ER S A+F CP  +  I+ +  P  YR+FS++EY++Q E DV++TG+KVGLSRF 
Sbjct: 276 SPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFL 335

Query: 181 L 182
           +
Sbjct: 336 I 336

[Close]

BLAST of CcUC10G191540 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 9.5e-45
Identity = 83/190 (43.68%), Postives = 125/190 (65.79%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSL-------GIKSSYFKERCEKGKSSFRLNRYPPCPFAS 60
           M+ V + + R+A  ++  L + L          + +F   C++     RLNRYP CPFA+
Sbjct: 166 MQEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAA 225

Query: 61  KVYGLIPHTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFR 120
             +GL+PHTDSD+LT+L Q Q+ GL L++  +W  V+P P AL+VNIGDLFQ  SN+ ++
Sbjct: 226 DTFGLVPHTDSDFLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYK 285

Query: 121 SLKHRVVASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNK 180
           S++H+VVA+   +R S AYF CPS D L+ +  +PS YR F++ EYR+++++DV  TG K
Sbjct: 286 SVEHKVVANAKTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKK 345

Query: 181 VGLSRFFLHN 184
           +GL  FF H+
Sbjct: 346 IGLPNFFKHS 355

[Close]

BLAST of CcUC10G191540 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 87/187 (46.52%), Postives = 119/187 (63.64%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSL-GIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLI 60
           +E V + +  LA+K++EIL + L G           +     RLNRYPPC  A   +GL 
Sbjct: 152 IEEVSRAMYELAQKLAEILMRGLPGAGEGETMVTTREETCFLRLNRYPPCAMAMGGFGLC 211

Query: 61  PHTDSDYLTILYQPQ--ISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKH 120
           PHTDSD LTI++Q Q  + GLQLL+ G+W  VKP+P  L+VN+GDL Q  SNDV++S++H
Sbjct: 212 PHTDSDLLTIVHQQQDTVGGLQLLKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEH 271

Query: 121 RVVASEAVERFSFAYFYCPSDDVLI-----ESWLKPSNYRQFSYKEYRQQIEKDVEKTGN 180
           RV+A+  +ERFS A+F CPS   LI           ++YR F++ EYR+QI +DV  TG 
Sbjct: 272 RVMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTGR 331

[Close]

BLAST of CcUC10G191540 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 4.8e-28
Identity = 60/135 (44.44%), Postives = 82/135 (60.74%), Query Frame = 0


Query: 8   LGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYL 67
           +  L+ KI E+LG SLG+K  YF+E  E+  S  RLN YPPC       G  PH D   L
Sbjct: 194 MSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSL 253

Query: 68  TILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVVASEAVER 127
           TIL+Q  ++GLQ+    +W  ++PNP+A +VNIGD F  +SND ++S  HR V +   ER
Sbjct: 254 TILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESER 313

Query: 128 FSFAYFYCPSDDVLI 143
            S A+F CP  D ++
Sbjct: 314 KSLAFFLCPKKDRVV 328

[Close]

BLAST of CcUC10G191540 vs. ExPASy TrEMBL
Match: A0A5D3C080 (Gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00430 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.0e-94
Identity = 167/188 (88.83%), Postives = 181/188 (96.28%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKLG LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 1   MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 60

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQP ISGLQL+++ KWFPVKPNPQALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 61  HTDTDYLTILYQPHISGLQLMKSAKWFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVV 120

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           AS  VER+SFAYFYCPSDDV+IESWLKPS Y+QFSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 121 ASVGVERYSFAYFYCPSDDVMIESWLKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180

Query: 181 LHNMLLPH 189
           LHN++LPH
Sbjct: 181 LHNIILPH 188

[Close]

BLAST of CcUC10G191540 vs. ExPASy TrEMBL
Match: A0A0A0L560 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081890 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.0e-94
Identity = 167/188 (88.83%), Postives = 180/188 (95.74%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKLG LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 1   MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 60

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQPQISGLQL ++ KWFPVKPNP+ALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 61  HTDTDYLTILYQPQISGLQLKKSAKWFPVKPNPRALLLNIGDLFQVVSNDIFRSLKHRVV 120

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VER+SFAYFYCPSDDV+IESWLKPS Y+ FSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 121 ASEGVERYSFAYFYCPSDDVIIESWLKPSIYKHFSYKEYRQQIEKDVEKTGNKVGLSRFF 180

Query: 181 LHNMLLPH 189
           LHN+ LPH
Sbjct: 181 LHNIALPH 188

[Close]

BLAST of CcUC10G191540 vs. ExPASy TrEMBL
Match: A0A6J1JAT6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483296 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.1e-91
Identity = 169/191 (88.48%), Postives = 178/191 (93.19%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME VVKK GRLAE I+EILGQSLGIKSSYFKERCEKG+SSFRLNRYPPCPFAS+VYGLIP
Sbjct: 160 MEAVVKKFGRLAESIAEILGQSLGIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIP 219

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQ Q+SGLQL++ GKWF VKPNP+ALLVNIGDLFQVVSND FRSLKHRVV
Sbjct: 220 HTDSDYLTILYQAQVSGLQLMKDGKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVV 279

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASEAVERFSFAYFY PSDDV+IES+ KPS YRQFSYKEYRQQIEKDVEKTGNKVGL RF 
Sbjct: 280 ASEAVERFSFAYFYSPSDDVMIESYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFL 339

Query: 181 LHNMLLPHLGQ 192
           LHNM LPHLGQ
Sbjct: 340 LHNM-LPHLGQ 349

[Close]

BLAST of CcUC10G191540 vs. ExPASy TrEMBL
Match: A0A6J1F0S8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438457 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.2e-90
Identity = 167/191 (87.43%), Postives = 177/191 (92.67%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME VVKK GRLAE I+EILGQSLGIK+ YFKERCEKG+SSFRLNRYPPCPFAS+VYGLIP
Sbjct: 160 MEAVVKKFGRLAESIAEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIP 219

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQ Q+SGLQL++ GKWF VKPNP+ALLVNIGDLFQVVSND FRSLKHRVV
Sbjct: 220 HTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVV 279

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASEAVERFSFAYFY PSDDV+IES  KPS YRQFSY+EYRQQIEKDVEKTGNKVGL RFF
Sbjct: 280 ASEAVERFSFAYFYSPSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFF 339

Query: 181 LHNMLLPHLGQ 192
           LHNM LPHLGQ
Sbjct: 340 LHNM-LPHLGQ 349

[Close]

BLAST of CcUC10G191540 vs. ExPASy TrEMBL
Match: A0A1S3CJB6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103501620 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 3.8e-89
Identity = 160/178 (89.89%), Postives = 172/178 (96.63%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKLG LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 156 MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 215

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQPQISGLQL+++ KWFPVKPNPQALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 216 HTDTDYLTILYQPQISGLQLMKSAKWFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVV 275

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSR 179
           AS  VER+SFAYFYCPSDDV+IESWLKPS Y+QFSYKEYRQQIEKDVEKTGNKVGLSR
Sbjct: 276 ASVGVERYSFAYFYCPSDDVMIESWLKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSR 333

[Close]

BLAST of CcUC10G191540 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 209.5 bits (532), Expect = 2.3e-54
Identity = 97/182 (53.30%), Postives = 130/182 (71.43%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME    +   LA  ++E+L +  G  SS+FKE C +     R+NRYPPCP  S+VYGL+P
Sbjct: 155 MEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMP 214

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSD+LTILYQ Q+ GLQL++  +W  VKPNP+AL++NIGDLFQ  SN +++S++HRV+
Sbjct: 215 HTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVM 274

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
            +  VERFS AYF CPS D +IE       YR FS++E+RQQ+++DV+K G KVGL RF 
Sbjct: 275 TNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

Query: 181 LH 183
            H
Sbjct: 335 NH 336

[Close]

BLAST of CcUC10G191540 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 186.0 bits (471), Expect = 2.7e-47
Identity = 87/181 (48.07%), Postives = 128/181 (70.72%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           +E  V+++ R+A+ I EILG+ + + S YF+   E   S  RLN+Y P  F S+V+GL+P
Sbjct: 156 VETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVP 215

Query: 61  HTDSDYLTILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+ +LTIL Q QI GL+L   G+W  VKP  +AL VNIGD+FQ +SN V++S++HRV+
Sbjct: 216 HTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVI 275

Query: 121 ASEAVERFSFAYFYCPSDDVLIESWLKPSNYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           +   +ER S A+F CP  +  I+ +  P  YR+FS++EY++Q E DV++TG+KVGLSRF 
Sbjct: 276 SPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFL 335

Query: 181 L 182
           +
Sbjct: 336 I 336

[Close]

BLAST of CcUC10G191540 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 125.9 bits (315), Expect = 3.4e-29
Identity = 60/135 (44.44%), Postives = 82/135 (60.74%), Query Frame = 0


Query: 8   LGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYL 67
           +  L+ KI E+LG SLG+K  YF+E  E+  S  RLN YPPC       G  PH D   L
Sbjct: 194 MSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSL 253

Query: 68  TILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVVASEAVER 127
           TIL+Q  ++GLQ+    +W  ++PNP+A +VNIGD F  +SND ++S  HR V +   ER
Sbjct: 254 TILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESER 313

Query: 128 FSFAYFYCPSDDVLI 143
            S A+F CP  D ++
Sbjct: 314 KSLAFFLCPKKDRVV 328

[Close]

BLAST of CcUC10G191540 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 61/168 (36.31%), Postives = 97/168 (57.74%), Query Frame = 0


Query: 1   MEGVVKKLGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           +EG  K++  L +++ + +  SLG+K   F+E   +   + R+N YPPC     V GL P
Sbjct: 175 LEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSP 234

Query: 61  HTDSDYLTILYQPQIS--GLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHR 120
           H+D   LT+L Q + S  GLQ+L+   W PVKP P AL++NIGD  +V+SN  ++S++HR
Sbjct: 235 HSDGSALTVLQQSKNSCVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHR 294

Query: 121 VVASEAVERFSFAYFYCPSDDV-------LIESWLKPSNYRQFSYKEY 160
            V +   ER +   FY P+ +V       L++    P  YR +++ +Y
Sbjct: 295 AVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDY 342

[Close]

BLAST of CcUC10G191540 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 61/164 (37.20%), Postives = 90/164 (54.88%), Query Frame = 0


Query: 8   LGRLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYL 67
           +  L+ KI E+LG SLG+   YF+   E+  S  RLN YPPC       G  PH D   L
Sbjct: 192 MSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSL 251

Query: 68  TILYQPQISGLQLLRAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVVASEAVER 127
           TIL+Q  ++GLQ+    +W  ++PNP+A +VNIGD F  +SN +F+S  HR V +    R
Sbjct: 252 TILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESAR 311

Query: 128 FSFAYFYCPSDDVLIE------SWLKPSNYRQFSYKEYRQQIEK 166
            S A+F CP  D +++        +K   Y  F++  + +  +K
Sbjct: 312 KSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQK 355

The following BLAST results are available for this feature:

BLAST of CcUC10G191540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906005.1	2.9e-99	94.24	gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida]	[more]
TYK04745.1	2.2e-94	88.83	gibberellin 2-beta-dioxygenase 8-like [Cucumis melo var. makuwa]	[more]
XP_004152933.2	6.3e-94	88.83	gibberellin 2-beta-dioxygenase 8 [Cucumis sativus] >XP_031737838.1 gibberellin 2...	[more]
XP_022985245.1	2.2e-91	88.48	gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima]	[more]
XP_023520421.1	6.5e-91	87.96	gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CcUC10G191540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	3.3e-53	53.30	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C6I4	3.9e-46	48.07	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q7XP65	9.5e-45	43.68	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q8LGZ9	1.2e-39	46.52	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q39110	4.8e-28	44.44	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]

BLAST of CcUC10G191540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C080	1.0e-94	88.83	Gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0L560	3.0e-94	88.83	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A6J1JAT6	1.1e-91	88.48	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148329...	[more]
A0A6J1F0S8	1.2e-90	87.43	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438...	[more]
A0A1S3CJB6	3.8e-89	89.89	gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103501620 PE...	[more]

BLAST of CcUC10G191540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	2.3e-54	53.30	gibberellin 2-oxidase 8	[more]
AT1G50960.1	2.7e-47	48.07	gibberellin 2-oxidase 7	[more]
AT4G25420.1	3.4e-29	44.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	2.9e-28	36.31	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51810.1	2.9e-28	37.20	gibberellin 20 oxidase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 41..136 e-value: 2.3E-22 score: 79.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..178 e-value: 2.2E-52 score: 180.3
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 2..175
None	No IPR available	PANTHER	PTHR47990:SF19	20-OXIDASE, PUTATIVE-RELATED	coord: 2..175
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 3..163
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 37..136 score: 12.201501

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC10G191540.1	CcUC10G191540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding