CcUC10G189920 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC10G189920
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	subtilisin-like protease SBT5.3
Location	CicolChr10: 4918971 .. 4920181 (+)
RNA-Seq Expression	CcUC10G189920
Synteny	CcUC10G189920

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTCTTTCCCTCAAGAGACCAGAAGAAATATGAATGGACAATTAAATAATGGACTTGCCACAATTTCCAGACGAACTTATAAAGAATTCTTACACAGGGAAATTTGTGATGAGGGAACGCTTAATTTCACGAAATTGAATGGGAAGATTGTGGTTTGCCTTCGAGGGGTTAACTCAAGAGTGGCCAAAGGTTAAGTAGCTGCTCAGGCAGGGGCTGTTGGGATGATTCTGGCTAACGATTAGCTAAGTGGGAATGAGATTTTAGCTGATGCACACATCATTCCAGCTTCCAGTGTAAACTATAATGATAGCATATCCATCTCCCAATATATCAGCTCTACAACGTAACTTCCTATATTTTCTTGTTTTTTTCCAAAGATTGCATAAATATTTTGTTTCCTCTACAAGTTTGAATTCATATTAACATGTACTTGATCAGGACACCAATGGCTTACATAAGTCCTGCGAGGAAGACACTTGGAGTCAAGCCCGCCCCAACAATGGCTGATTTCTCAGGAAGAGGCCCTAATACAATCGAGGAGTCAATCCTCAAGGTTTATTTTTCTCAACCAAGATTTGTTATATGAAACATTTCAGTTTAAATGCTTCCGTTCAAAATACATGCAAACTTAAGCTTAGTAATCAACAAAATCAAGCTAATGTAAAGATAAGGAAGATCATTAATCCTTACAAGTTGTGTTTTATCAGCCAGATGTAACAGCACCAGGTGTGAATATAATGGCAGCTTACCCCAGTGGAATACCGTTGTCAAATATACCAGTTGACGATCGTCGAGTTCCTTTTAAGGTAGACTCTGGCATATCCATGGTCCGCCCCCATGTTTCAGGCATTGTAGGCCTTCTCAAAACCCTAAATCCAAAATGGAGTCCAGCCGCCATTAAATCTGCAATCATGACTACAGGTTCAATCATACAATCAAAAGGGGCCCTGTGACGATCAATCGAAAGGTGAAGAATGTGGGAAGTCCAGGGAGGTATGTTGCTCGAGTGAAGGCGCCTTCGGAAGTTTCAATTGTTGTGGAGACAAGTACATCGGAATTTGCCGCCATGGATGAAGAGAATAGCTTCAAGGTTGTGTTGCAAAGCACTGGAAAGGGAAATGAAGAGGGTTATGCGTTTGGAACATTGGTTTGGTCAGATAACAAGCACAATGTTAGAAGCCCAATTGTTGTTAAGTTGAGGGAATAA

mRNA sequence

ATGCTTTCTTTCCCTCAAGAGACCAGAAGAAATATGAATGGACAATTAAATAATGGACTTGCCACAATTTCCAGACGAACTTATAAAGAATTCTTACACAGGGAAATTTGTGATGAGGGAACGCTTAATTTCACGAAATTGAATGGGAAGATTGTGGTTTGCCTTCGAGGGGTTAACTCAAGAGTGGCCAAAGCTGATGCACACATCATTCCAGCTTCCAGTGTAAACTATAATGATAGCATATCCATCTCCCAATATATCAGCTCTACAACGACACCAATGGCTTACATAAGTCCTGCGAGGAAGACACTTGGAGTCAAGCCCGCCCCAACAATGGCTGATTTCTCAGGAAGAGGCCCTAATACAATCGAGGAGTCAATCCTCAAGCCAGATGTAACAGCACCAGGTGTGAATATAATGGCAGCTTACCCCAGTGGAATACCGTTGTCAAATATACCAGTTGACGATCGTCGAGTTCCTTTTAAGGTAGACTCTGGCATATCCATGGTCCGCCCCCATGTTTCAGGCATTGTAGGCCTTCTCAAAACCCTAAATCCAAAATGGAGTCCAGCCGCCATTAAATCTGCAATCATGACTACAGGGAGGTATGTTGCTCGAGTGAAGGCGCCTTCGGAAGTTTCAATTGTTGTGGAGACAAGTACATCGGAATTTGCCGCCATGGATGAAGAGAATAGCTTCAAGGTTGTGTTGCAAAGCACTGGAAAGGGAAATGAAGAGGGTTATGCGTTTGGAACATTGGTTTGGTCAGATAACAAGCACAATGTTAGAAGCCCAATTGTTGTTAAGTTGAGGGAATAA

Coding sequence (CDS)

ATGCTTTCTTTCCCTCAAGAGACCAGAAGAAATATGAATGGACAATTAAATAATGGACTTGCCACAATTTCCAGACGAACTTATAAAGAATTCTTACACAGGGAAATTTGTGATGAGGGAACGCTTAATTTCACGAAATTGAATGGGAAGATTGTGGTTTGCCTTCGAGGGGTTAACTCAAGAGTGGCCAAAGCTGATGCACACATCATTCCAGCTTCCAGTGTAAACTATAATGATAGCATATCCATCTCCCAATATATCAGCTCTACAACGACACCAATGGCTTACATAAGTCCTGCGAGGAAGACACTTGGAGTCAAGCCCGCCCCAACAATGGCTGATTTCTCAGGAAGAGGCCCTAATACAATCGAGGAGTCAATCCTCAAGCCAGATGTAACAGCACCAGGTGTGAATATAATGGCAGCTTACCCCAGTGGAATACCGTTGTCAAATATACCAGTTGACGATCGTCGAGTTCCTTTTAAGGTAGACTCTGGCATATCCATGGTCCGCCCCCATGTTTCAGGCATTGTAGGCCTTCTCAAAACCCTAAATCCAAAATGGAGTCCAGCCGCCATTAAATCTGCAATCATGACTACAGGGAGGTATGTTGCTCGAGTGAAGGCGCCTTCGGAAGTTTCAATTGTTGTGGAGACAAGTACATCGGAATTTGCCGCCATGGATGAAGAGAATAGCTTCAAGGTTGTGTTGCAAAGCACTGGAAAGGGAAATGAAGAGGGTTATGCGTTTGGAACATTGGTTTGGTCAGATAACAAGCACAATGTTAGAAGCCCAATTGTTGTTAAGTTGAGGGAATAA

Protein sequence

MLSFPQETRRNMNGQLNNGLATISRRTYKEFLHREICDEGTLNFTKLNGKIVVCLRGVNSRVAKADAHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSPAAIKSAIMTTGRYVARVKAPSEVSIVVETSTSEFAAMDEENSFKVVLQSTGKGNEEGYAFGTLVWSDNKHNVRSPIVVKLRE

Homology

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BLAST of CcUC10G189920 vs. NCBI nr
Match: XP_038904160.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 331.3 bits (848), Expect = 8.1e-87
Identity = 199/370 (53.78%), Postives = 212/370 (57.30%), Query Frame = 0


Query: 35  EICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHI 94
           EICD+GTL+FTKLNGKIVVCLRGVNSRVAK                         AD HI
Sbjct: 429 EICDDGTLDFTKLNGKIVVCLRGVNSRVAKGYVAVQAGAVGMILVNDEQSGNEILADPHI 488

Query: 95  IPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILK 154
           IP S+VNYNDSISISQYISST +PMAYIS  R TLGVKPAP MA FS RGPNTIEESILK
Sbjct: 489 IPTSNVNYNDSISISQYISSTMSPMAYISSVRTTLGVKPAPKMAGFSARGPNTIEESILK 548

Query: 155 PDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWS 214
           PD+TAPGVNI+AAYP GIPLS +PVDDRR+PFKVDSG SM  PHVSGIVGLLKTL PKWS
Sbjct: 549 PDITAPGVNIIAAYPDGIPLSKLPVDDRRLPFKVDSGTSMACPHVSGIVGLLKTLYPKWS 608

Query: 215 PAAIKSAIMTT------------------------------------------------- 271
           PAAIKSAIMTT                                                 
Sbjct: 609 PAAIKSAIMTTAKTRDGKLHPIVDSNGLKATPLAYGAGHINPNSAMDPGLVYDIAIDDYL 668

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BLAST of CcUC10G189920 vs. NCBI nr
Match: XP_023553406.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 300.4 bits (768), Expect = 1.5e-77
Identity = 183/372 (49.19%), Postives = 203/372 (54.57%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVA-------------------------KAD 91
           L  E+CDEGTL+ TKLNGKIVVC+ GV +RV                          KAD
Sbjct: 393 LAAEVCDEGTLDPTKLNGKIVVCVVGVIARVTKGYLAAQAGAVGMILVNDEESGNEIKAD 452

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            HIIPAS V YNDSI+ISQY+SST TPMAYIS     LGV PAPTMA FSGRGP+ IEES
Sbjct: 453 PHIIPASHVTYNDSITISQYMSSTRTPMAYISSVTAKLGVTPAPTMARFSGRGPSIIEES 512

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+TAPGVNI+AAYP GIPL+N+PVDDRRVPF V SG SM  PHVSGIVGLLKTLNP
Sbjct: 513 ILKPDITAPGVNIIAAYPDGIPLANLPVDDRRVPFMVYSGTSMSCPHVSGIVGLLKTLNP 572

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
           KWSPAAIKSAIMTT                                              
Sbjct: 573 KWSPAAIKSAIMTTAKTRDSSLHPIVDLDGVIATPLAYGAGHVHPNSAMDPGLVYDITMD 632

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BLAST of CcUC10G189920 vs. NCBI nr
Match: XP_022965240.1 (subtilisin-like protease SBT5.3 [Cucurbita maxima])

HSP 1 Score: 299.7 bits (766), Expect = 2.6e-77
Identity = 183/372 (49.19%), Postives = 203/372 (54.57%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVA-------------------------KAD 91
           L  EIC+EGTL+ +KLNGKIVVCL GVN+RVA                         KAD
Sbjct: 393 LDAEICEEGTLDSSKLNGKIVVCLVGVNARVAKGYVAAQAGAIGMILVNDEESGNEIKAD 452

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            HIIPAS V YNDSI+ISQYISST TPMAYIS     LGV PAPT+A FSGRGP+ IEES
Sbjct: 453 PHIIPASHVTYNDSITISQYISSTRTPMAYISSVTAKLGVTPAPTIARFSGRGPSIIEES 512

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+TAPGVNI+AAYP GIPL+N+PVDDRRVPF V SG SM  PHVSGIVGLLKTLNP
Sbjct: 513 ILKPDITAPGVNIIAAYPDGIPLANLPVDDRRVPFMVYSGTSMSCPHVSGIVGLLKTLNP 572

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
           KWSPAAIKSAIMTT                                              
Sbjct: 573 KWSPAAIKSAIMTTAKTRDSTLHPIVDLDGVIATPLAYGAGHVHPNSAMDPGLVYDITID 632

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BLAST of CcUC10G189920 vs. NCBI nr
Match: KAG6577532.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 295.0 bits (754), Expect = 6.4e-76
Identity = 180/372 (48.39%), Postives = 202/372 (54.30%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVA-------------------------KAD 91
           L  EIC+EGTL+ +KLNGKIVVCL GVN+R+A                         KAD
Sbjct: 367 LDAEICEEGTLDSSKLNGKIVVCLVGVNARMAKGYVAAQAGAVGMILVNNQQSWNEIKAD 426

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            HIIPAS V YNDSI+ISQYISST TPMAYIS     LGV PAPT+A FSGRGP+ IEES
Sbjct: 427 PHIIPASHVTYNDSITISQYISSTRTPMAYISSVTAKLGVMPAPTVARFSGRGPSIIEES 486

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+ APGVNI+AAYP GIPL+N+PVDDRR PF V SG SM  PHVSGIVGLLKTLNP
Sbjct: 487 ILKPDIIAPGVNIIAAYPDGIPLANLPVDDRRGPFMVYSGTSMSCPHVSGIVGLLKTLNP 546

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
           KWSPA IKSAIMTT                                              
Sbjct: 547 KWSPATIKSAIMTTAKTRDSSLHPIVDLDGVIATPLAYGAGHVHPNSAMDPGLVYDITID 606

[Close]

BLAST of CcUC10G189920 vs. NCBI nr
Match: XP_022932424.1 (subtilisin-like protease SBT5.3 [Cucurbita moschata])

HSP 1 Score: 293.1 bits (749), Expect = 2.4e-75
Identity = 181/372 (48.66%), Postives = 201/372 (54.03%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVA-------------------------KAD 91
           L  E+CDEGTL+ TKLNGKIVVC+ GV +RV                          KAD
Sbjct: 392 LAAEVCDEGTLDPTKLNGKIVVCVVGVIARVTKGYLAAQAGAVGMILVNDEESGNEIKAD 451

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            HIIPAS V YNDSI+ISQYISST TPMAYIS     LGV PAPT+A FSGRGP+ IEES
Sbjct: 452 PHIIPASHVTYNDSITISQYISSTRTPMAYISSVTAKLGVTPAPTVARFSGRGPSIIEES 511

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+ APGVNI+AAYP GIPL+N+PVDDRR PF V SG SM  PHVSGIVGLLKTLNP
Sbjct: 512 ILKPDIIAPGVNIIAAYPDGIPLANLPVDDRRGPFMVYSGTSMSCPHVSGIVGLLKTLNP 571

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
           KWSPAAIKSAIMTT                                              
Sbjct: 572 KWSPAAIKSAIMTTAKTRDSSLHPIVDLDGVIATPLAYGAGHVHPNSAMDPGLVYDITID 631

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BLAST of CcUC10G189920 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.2e-45
Identity = 124/373 (33.24%), Postives = 164/373 (43.97%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------AD 91
           L  ++C  G+L+  K  GKI+VCLRG N RV K                         AD
Sbjct: 400 LDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLAD 459

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            H++PA+ +   DS ++S+YIS T  P+A+I+P+R  LG+KPAP MA FS +GP+ +   
Sbjct: 460 PHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQ 519

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+TAPGV+++AAY   +  +N   D RR+ F   SG SM  PH+SGI GLLKT  P
Sbjct: 520 ILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYP 579

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
            WSPAAI+SAIMTT                                              
Sbjct: 580 SWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIK 639

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BLAST of CcUC10G189920 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.1e-45
Identity = 95/192 (49.48%), Postives = 122/192 (63.54%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C +G+L+  K+ GKI+VCLRG N+RV K                         +DAH++
Sbjct: 410 LCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVL 469

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PAS ++Y D  ++  Y+SST  P  YI     TL  KPAP MA FS RGPNTI   ILKP
Sbjct: 470 PASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKP 529

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 203
           D+TAPGVNI+AA+      +++  D+RR PF  +SG SM  PH+SG+VGLLKTL+P WSP
Sbjct: 530 DITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSP 589

[Close]

BLAST of CcUC10G189920 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.6e-35
Identity = 84/190 (44.21%), Postives = 114/190 (60.00%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C  GTL   K+ GKIV+C RG+N+RV K                         ADAH++
Sbjct: 385 LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLL 444

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PA++V       I  Y+++   P A IS     +GVKP+P +A FS RGPN+I  +ILKP
Sbjct: 445 PATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKP 504

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 201
           D+ APGVNI+AA+      + +  D RRV F + SG SM  PHVSG+  LLK+++P+WSP
Sbjct: 505 DLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSP 564

[Close]

BLAST of CcUC10G189920 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.4e-35
Identity = 84/194 (43.30%), Postives = 110/194 (56.70%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C E TL+  ++ GKIV+C RG + RVAK                          DAH+I
Sbjct: 381 LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLI 440

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PA +V  N+   I  Y SS   P+A I      +G+KPAP +A FSGRGPN +   ILKP
Sbjct: 441 PACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKP 500

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 205
           D+ APGVNI+AA+   +  + +P D R+  F + SG SM  PHVSG   LLK+ +P WSP
Sbjct: 501 DLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP 560

[Close]

BLAST of CcUC10G189920 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-34
Identity = 82/200 (41.00%), Postives = 112/200 (56.00%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C  G+L+ + + GKIVVC RGVN+RV K                         AD+H++
Sbjct: 379 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 438

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PA +V       + +Y+ S + P A +      L VKP+P +A FS RGPNT+   ILKP
Sbjct: 439 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKP 498

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 211
           DV  PGVNI+A +   I  + +  D RR  F + SG SM  PH+SG+ GLLK  +P+WSP
Sbjct: 499 DVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSP 558

[Close]

BLAST of CcUC10G189920 vs. ExPASy TrEMBL
Match: A0A6J1HN63 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111465152 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 1.3e-77
Identity = 183/372 (49.19%), Postives = 203/372 (54.57%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVA-------------------------KAD 91
           L  EIC+EGTL+ +KLNGKIVVCL GVN+RVA                         KAD
Sbjct: 393 LDAEICEEGTLDSSKLNGKIVVCLVGVNARVAKGYVAAQAGAIGMILVNDEESGNEIKAD 452

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            HIIPAS V YNDSI+ISQYISST TPMAYIS     LGV PAPT+A FSGRGP+ IEES
Sbjct: 453 PHIIPASHVTYNDSITISQYISSTRTPMAYISSVTAKLGVTPAPTIARFSGRGPSIIEES 512

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+TAPGVNI+AAYP GIPL+N+PVDDRRVPF V SG SM  PHVSGIVGLLKTLNP
Sbjct: 513 ILKPDITAPGVNIIAAYPDGIPLANLPVDDRRVPFMVYSGTSMSCPHVSGIVGLLKTLNP 572

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
           KWSPAAIKSAIMTT                                              
Sbjct: 573 KWSPAAIKSAIMTTAKTRDSTLHPIVDLDGVIATPLAYGAGHVHPNSAMDPGLVYDITID 632

[Close]

BLAST of CcUC10G189920 vs. ExPASy TrEMBL
Match: A0A6J1EWY3 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111438777 PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 1.2e-75
Identity = 181/372 (48.66%), Postives = 201/372 (54.03%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVA-------------------------KAD 91
           L  E+CDEGTL+ TKLNGKIVVC+ GV +RV                          KAD
Sbjct: 392 LAAEVCDEGTLDPTKLNGKIVVCVVGVIARVTKGYLAAQAGAVGMILVNDEESGNEIKAD 451

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            HIIPAS V YNDSI+ISQYISST TPMAYIS     LGV PAPT+A FSGRGP+ IEES
Sbjct: 452 PHIIPASHVTYNDSITISQYISSTRTPMAYISSVTAKLGVTPAPTVARFSGRGPSIIEES 511

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+ APGVNI+AAYP GIPL+N+PVDDRR PF V SG SM  PHVSGIVGLLKTLNP
Sbjct: 512 ILKPDIIAPGVNIIAAYPDGIPLANLPVDDRRGPFMVYSGTSMSCPHVSGIVGLLKTLNP 571

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
           KWSPAAIKSAIMTT                                              
Sbjct: 572 KWSPAAIKSAIMTTAKTRDSSLHPIVDLDGVIATPLAYGAGHVHPNSAMDPGLVYDITID 631

[Close]

BLAST of CcUC10G189920 vs. ExPASy TrEMBL
Match: A0A5D3D737 (Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00080 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.4e-68
Identity = 167/371 (45.01%), Postives = 192/371 (51.75%), Query Frame = 0


Query: 35  EICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHI 94
           E+CDEG+L+  KL GKIVVCLRG  SRV+K                          D+H+
Sbjct: 378 EVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITDSHV 437

Query: 95  IPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILK 154
           +PAS V Y+DSISI QYI+ST  P AYIS     L + P+  +ADFS RGPNTIEESILK
Sbjct: 438 LPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEESILK 497

Query: 155 PDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWS 214
           PD+TAPGVNI+AAYP GIPL+  P+DDR+ PFKVDSG SM  PHV+GIVGLLKTLNPKWS
Sbjct: 498 PDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNPKWS 557

Query: 215 PAAIKSAIMTT------------------------------------------------- 273
           PAAIKSAIMTT                                                 
Sbjct: 558 PAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYL 617

[Close]

BLAST of CcUC10G189920 vs. ExPASy TrEMBL
Match: A0A1S3BL27 (subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491006 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.4e-68
Identity = 167/371 (45.01%), Postives = 192/371 (51.75%), Query Frame = 0


Query: 35  EICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHI 94
           E+CDEG+L+  KL GKIVVCLRG  SRV+K                          D+H+
Sbjct: 311 EVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITDSHV 370

Query: 95  IPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILK 154
           +PAS V Y+DSISI QYI+ST  P AYIS     L + P+  +ADFS RGPNTIEESILK
Sbjct: 371 LPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEESILK 430

Query: 155 PDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWS 214
           PD+TAPGVNI+AAYP GIPL+  P+DDR+ PFKVDSG SM  PHV+GIVGLLKTLNPKWS
Sbjct: 431 PDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNPKWS 490

Query: 215 PAAIKSAIMTT------------------------------------------------- 273
           PAAIKSAIMTT                                                 
Sbjct: 491 PAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYL 550

[Close]

BLAST of CcUC10G189920 vs. ExPASy TrEMBL
Match: A0A1S3BLZ2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491006 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.4e-68
Identity = 167/371 (45.01%), Postives = 192/371 (51.75%), Query Frame = 0


Query: 35  EICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHI 94
           E+CDEG+L+  KL GKIVVCLRG  SRV+K                          D+H+
Sbjct: 340 EVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITDSHV 399

Query: 95  IPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILK 154
           +PAS V Y+DSISI QYI+ST  P AYIS     L + P+  +ADFS RGPNTIEESILK
Sbjct: 400 LPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEESILK 459

Query: 155 PDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWS 214
           PD+TAPGVNI+AAYP GIPL+  P+DDR+ PFKVDSG SM  PHV+GIVGLLKTLNPKWS
Sbjct: 460 PDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNPKWS 519

Query: 215 PAAIKSAIMTT------------------------------------------------- 273
           PAAIKSAIMTT                                                 
Sbjct: 520 PAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYL 579

[Close]

BLAST of CcUC10G189920 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 184.9 bits (468), Expect = 8.7e-47
Identity = 124/373 (33.24%), Postives = 164/373 (43.97%), Query Frame = 0


Query: 32  LHREICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------AD 91
           L  ++C  G+L+  K  GKI+VCLRG N RV K                         AD
Sbjct: 400 LDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLAD 459

Query: 92  AHIIPASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEES 151
            H++PA+ +   DS ++S+YIS T  P+A+I+P+R  LG+KPAP MA FS +GP+ +   
Sbjct: 460 PHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQ 519

Query: 152 ILKPDVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNP 211
           ILKPD+TAPGV+++AAY   +  +N   D RR+ F   SG SM  PH+SGI GLLKT  P
Sbjct: 520 ILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYP 579

Query: 212 KWSPAAIKSAIMTT---------------------------------------------- 271
            WSPAAI+SAIMTT                                              
Sbjct: 580 SWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIK 639

[Close]

BLAST of CcUC10G189920 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 182.6 bits (462), Expect = 4.3e-46
Identity = 95/192 (49.48%), Postives = 122/192 (63.54%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C +G+L+  K+ GKI+VCLRG N+RV K                         +DAH++
Sbjct: 410 LCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVL 469

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PAS ++Y D  ++  Y+SST  P  YI     TL  KPAP MA FS RGPNTI   ILKP
Sbjct: 470 PASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKP 529

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 203
           D+TAPGVNI+AA+      +++  D+RR PF  +SG SM  PH+SG+VGLLKTL+P WSP
Sbjct: 530 DITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSP 589

HSP 2 Score: 46.6 bits (109), Expect = 3.7e-05
Identity = 33/103 (32.04%), Postives = 48/103 (46.60%), Query Frame = 0


Query: 168 SMVRPHVSGIVGLLKTLNPKWSPAAIKSAIMTTGRYVARVKAPSEVSIVVETSTSEFAAM 227
           S+  P+++G + + + L     PA           Y AR + P  V + VE     F   
Sbjct: 685 SITVPNLTGSITVTRKLKNVGPPAT----------YNARFREPLGVRVSVEPKQLTFNKT 744

Query: 228 DEENSFKVVLQSTGKGNEEGYAFGTLVWSDNKHNVRSPIVVKL 271
            E   F++ L+        GY FG L W+D+ H VRSPIVV+L
Sbjct: 745 GEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

[Close]

BLAST of CcUC10G189920 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.8e-36
Identity = 84/190 (44.21%), Postives = 114/190 (60.00%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C  GTL   K+ GKIV+C RG+N+RV K                         ADAH++
Sbjct: 385 LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLL 444

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PA++V       I  Y+++   P A IS     +GVKP+P +A FS RGPN+I  +ILKP
Sbjct: 445 PATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKP 504

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 201
           D+ APGVNI+AA+      + +  D RRV F + SG SM  PHVSG+  LLK+++P+WSP
Sbjct: 505 DLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSP 564

[Close]

BLAST of CcUC10G189920 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 150.2 bits (378), Expect = 2.4e-36
Identity = 84/194 (43.30%), Postives = 110/194 (56.70%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C E TL+  ++ GKIV+C RG + RVAK                          DAH+I
Sbjct: 381 LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLI 440

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PA +V  N+   I  Y SS   P+A I      +G+KPAP +A FSGRGPN +   ILKP
Sbjct: 441 PACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKP 500

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 205
           D+ APGVNI+AA+   +  + +P D R+  F + SG SM  PHVSG   LLK+ +P WSP
Sbjct: 501 DLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP 560

[Close]

BLAST of CcUC10G189920 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.5e-35
Identity = 82/200 (41.00%), Postives = 112/200 (56.00%), Query Frame = 0


Query: 36  ICDEGTLNFTKLNGKIVVCLRGVNSRVAK-------------------------ADAHII 95
           +C  G+L+ + + GKIVVC RGVN+RV K                         AD+H++
Sbjct: 379 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 438

Query: 96  PASSVNYNDSISISQYISSTTTPMAYISPARKTLGVKPAPTMADFSGRGPNTIEESILKP 155
           PA +V       + +Y+ S + P A +      L VKP+P +A FS RGPNT+   ILKP
Sbjct: 439 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKP 498

Query: 156 DVTAPGVNIMAAYPSGIPLSNIPVDDRRVPFKVDSGISMVRPHVSGIVGLLKTLNPKWSP 211
           DV  PGVNI+A +   I  + +  D RR  F + SG SM  PH+SG+ GLLK  +P+WSP
Sbjct: 499 DVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSP 558

The following BLAST results are available for this feature:

BLAST of CcUC10G189920 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904160.1	8.1e-87	53.78	subtilisin-like protease SBT5.3 [Benincasa hispida]	[more]
XP_023553406.1	1.5e-77	49.19	subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo]	[more]
XP_022965240.1	2.6e-77	49.19	subtilisin-like protease SBT5.3 [Cucurbita maxima]	[more]
KAG6577532.1	6.4e-76	48.39	Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022932424.1	2.4e-75	48.66	subtilisin-like protease SBT5.3 [Cucurbita moschata]	[more]

BLAST of CcUC10G189920 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSP5	1.2e-45	33.24	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
F4JXC5	6.1e-45	49.48	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
O65351	2.6e-35	44.21	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
O49607	3.4e-35	43.30	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]
Q9ZUF6	2.2e-34	41.00	Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S...	[more]

BLAST of CcUC10G189920 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HN63	1.3e-77	49.19	subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111465152 PE=3...	[more]
A0A6J1EWY3	1.2e-75	48.66	subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111438777 PE...	[more]
A0A5D3D737	1.4e-68	45.01	Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BL27	1.4e-68	45.01	subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034910...	[more]
A0A1S3BLZ2	1.4e-68	45.01	subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034910...	[more]

BLAST of CcUC10G189920 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	8.7e-47	33.24	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	4.3e-46	49.48	Subtilase family protein	[more]
AT5G67360.1	1.8e-36	44.21	Subtilase family protein	[more]
AT4G34980.1	2.4e-36	43.30	subtilisin-like serine protease 2	[more]
AT2G05920.1	1.5e-35	41.00	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 200..268 e-value: 2.1E-15 score: 56.7
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 68..203 e-value: 2.8E-14 score: 53.1
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 105..206 e-value: 6.5E-46 score: 159.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 110..205
None	No IPR available	GENE3D	3.50.30.30		coord: 34..104 e-value: 6.5E-46 score: 159.0
None	No IPR available	PANTHER	PTHR10795:SF662	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 30..64 coord: 201..271
None	No IPR available	PANTHER	PTHR10795:SF662	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 65..201
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 37..97 e-value: 6.91065E-9 score: 50.8751
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 30..64 coord: 65..201 coord: 201..271

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC10G189920.1	CcUC10G189920.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity