CcUC10G189910 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC10G189910
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionSubtilisin-like protease
LocationCicolChr10: 4917506 .. 4918957 (+)
RNA-Seq ExpressionCcUC10G189910
SyntenyCcUC10G189910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAGGATTTCCAAGTGTGATTTCGGTTTTTGAAAGTAAGGAAAGACAATTGCACACAACAAGATCATGGGATTTTCTTGGATTAGAAAGACAAGAAGCAGTTCCTTCAGACTCCATTTGGAATGTTACGAGGTTTGGTGATGAAGCAATTATAGCTAACTTTGACACAGGTTCTTTCTGTTCTTTCATTGCTTTTGTTTTTCGTTTGGCCTAAGTTGGTGTTATATTTTTCCGCTAAGAAGCAAAGTAATACTTTAATTTGGCTAGCATGTCTATGTCTGAGTACACATATCAAACACTTTTTGGTGCAATAGATGTGTAAAGACACTAATTATACAAAGTCAAAGTAGGCTCAACATTATTTGATTTAAACTATGATGTGTGTCATCGTGTCTGTGTCATATCATATCTGTATATGTGCTTCTTAAACATATAAATGCATAAACATTAAGAAATGTATATTTTAATCAACATGTTTATATTGCGTTCATGTTCATGATTTTAAAATATGATCACATGTCTTCATCATGTCCATGTAATACTCTTCATGTAATTGGAATATAGGTGTTTGGCCAGAATCTAAGAGCTTCAGTGACGAAGGATATGGGCCTGTCCCTTCAAGGTGGTTCGGCAGTTGTCAAAGTGACACTGACCCCAACTTTCACTGCAACAGGTCAAGAGCTTCAATTCGTCCCCTATCTATACTTTCTTCCATTGTTGTAGTAAATATGAAACAAGTACCTCAAATGCAGGAAGCTAGTTGGAGCAAGATTCTTTTAATAAAGCATATGGTCCGCTCAATGTCAGCTTTAATTCTTCAAGGGACCATCATGGCCACGGAACCCAGACTTTATCCACAGCTGGTGGCAATTTTGTCTCTGGGGCTAATGTTTTTGGCAATGGCAATGGAACGGCGAAAGGTGGCTCGCCTCGAGCCCGTGTTGCATCCTACAAGGTCTGTTGGCCAGCAGAAGATGGAGGGTGTTTTGACCCGGATATCTTAGCCGCCTTTGAAGCTGCCATTAGCGATGGAGTTGATGTCATCTCAGTTTCTCTAGGTGCAATTCCCACAGACTTTCTAAACGATGGACTATCCATAGGAGCCTTCCATGCAGTTCAGCAAGGCATCGTTGTTGTTTGCTCAGCTGGGAACTCAGGACCAGCTCCTGGATCGCCATGGATGTTAACTGTTGGGGCTAGTACCATTGATAGGGATTTCACCAATTTCGTGGTCCTTGGGAACAAGAAGCGATTCAAGGTACTTGTATCAAACACAACCTTTGTTAGAAAACTTAACTGAATAACATTTTCTATCATAACTAGCTATCTCCCAACTATCAAAATCTAGGGTGCAAGCCTTTCTTCTAAAGCATTGCCAGTTAACAAGTTCTACCCCTTAATTAATGCCGTGGATGTGAAAGCCAACAATGTTACTAACCGTGATGCATAA

mRNA sequence

ATGCTAGGATTTCCAAGTGTGATTTCGGTTTTTGAAAGTAAGGAAAGACAATTGCACACAACAAGATCATGGGATTTTCTTGGATTAGAAAGACAAGAAGCAGTTCCTTCAGACTCCATTTGGAATGTTACGAGGTTTGGTGATGAAGCAATTATAGCTAACTTTGACACAGTGACGAAGGATATGGGCCTGTCCCTTCAAGGTGGTTCGGCAGTTGTCAAAGTGACACTGACCCCAACTTTCACTGCAACAGGTCAAGAGCTTCAATTCGTCCCCTATCTATACTTTCTTCCATTGTTGTACTTTAATTCTTCAAGGGACCATCATGGCCACGGAACCCAGACTTTATCCACAGCTGGTGGCAATTTTGTCTCTGGGGCTAATGTTTTTGGCAATGGCAATGGAACGGCGAAAGGTGGCTCGCCTCGAGCCCGTGTTGCATCCTACAAGGTCTGTTGGCCAGCAGAAGATGGAGGGTGTTTTGACCCGGATATCTTAGCCGCCTTTGAAGCTGCCATTAGCGATGGAGTTGATGTCATCTCAGTTTCTCTAGGTGCAATTCCCACAGACTTTCTAAACGATGGACTATCCATAGGAGCCTTCCATGCAGTTCAGCAAGGCATCGTTGTTGTTTGCTCAGCTGGGAACTCAGGACCAGCTCCTGGATCGCCATGGATGTTAACTGTTGGGGCTAGTACCATTGATAGGGATTTCACCAATTTCGTGGTCCTTGGGAACAAGAAGCGATTCAAGGGTGCAAGCCTTTCTTCTAAAGCATTGCCAGTTAACAAGTTCTACCCCTTAATTAATGCCGTGGATGTGAAAGCCAACAATGTTACTAACCGTGATGCATAA

Coding sequence (CDS)

ATGCTAGGATTTCCAAGTGTGATTTCGGTTTTTGAAAGTAAGGAAAGACAATTGCACACAACAAGATCATGGGATTTTCTTGGATTAGAAAGACAAGAAGCAGTTCCTTCAGACTCCATTTGGAATGTTACGAGGTTTGGTGATGAAGCAATTATAGCTAACTTTGACACAGTGACGAAGGATATGGGCCTGTCCCTTCAAGGTGGTTCGGCAGTTGTCAAAGTGACACTGACCCCAACTTTCACTGCAACAGGTCAAGAGCTTCAATTCGTCCCCTATCTATACTTTCTTCCATTGTTGTACTTTAATTCTTCAAGGGACCATCATGGCCACGGAACCCAGACTTTATCCACAGCTGGTGGCAATTTTGTCTCTGGGGCTAATGTTTTTGGCAATGGCAATGGAACGGCGAAAGGTGGCTCGCCTCGAGCCCGTGTTGCATCCTACAAGGTCTGTTGGCCAGCAGAAGATGGAGGGTGTTTTGACCCGGATATCTTAGCCGCCTTTGAAGCTGCCATTAGCGATGGAGTTGATGTCATCTCAGTTTCTCTAGGTGCAATTCCCACAGACTTTCTAAACGATGGACTATCCATAGGAGCCTTCCATGCAGTTCAGCAAGGCATCGTTGTTGTTTGCTCAGCTGGGAACTCAGGACCAGCTCCTGGATCGCCATGGATGTTAACTGTTGGGGCTAGTACCATTGATAGGGATTTCACCAATTTCGTGGTCCTTGGGAACAAGAAGCGATTCAAGGGTGCAAGCCTTTCTTCTAAAGCATTGCCAGTTAACAAGTTCTACCCCTTAATTAATGCCGTGGATGTGAAAGCCAACAATGTTACTAACCGTGATGCATAA

Protein sequence

MLGFPSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVTKDMGLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYFLPLLYFNSSRDHHGHGTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAAISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPGSPWMLTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA
Homology
BLAST of CcUC10G189910 vs. NCBI nr
Match: XP_038904160.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 429.1 bits (1102), Expect = 3.0e-116
Identity = 230/297 (77.44%), Postives = 244/297 (82.15%), Query Frame = 0

Query: 4   FPSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KD 63
           +P+VISVFESKERQLHTTRSW+FLGLER+EAVPS+SIWNV RFGD+AIIANFDT    + 
Sbjct: 132 YPNVISVFESKERQLHTTRSWNFLGLERKEAVPSNSIWNVARFGDDAIIANFDTGVWPES 191

Query: 64  MGLSLQGGSAVVKVTL-------TPTFTATGQEL--QFVPYLYFLPLLYFNSSRDHHGHG 123
              S +G   V    L        P F    + +  +F    Y    + FNSSRDH GHG
Sbjct: 192 KSFSDEGYGPVPSRWLGSCQSDTDPNFHCNRKLIGARFFNKAYGPLNVSFNSSRDHDGHG 251

Query: 124 TQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAA 183
           T TLSTAGGNFVSGA VFGNGNGTAKGGSPRARVASYKVCWPAE GGCFDPDILAAFEAA
Sbjct: 252 THTLSTAGGNFVSGATVFGNGNGTAKGGSPRARVASYKVCWPAEGGGCFDPDILAAFEAA 311

Query: 184 ISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWML 243
           ISDGVDVISVSLGAIP+DFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG     SPWML
Sbjct: 312 ISDGVDVISVSLGAIPSDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPGTVANVSPWML 371

Query: 244 TVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           TVGASTIDRDFTNFVVLGNKKR KGASLSSKALPVNKFYPLINAVD KANNV+NRDA
Sbjct: 372 TVGASTIDRDFTNFVVLGNKKRLKGASLSSKALPVNKFYPLINAVDAKANNVSNRDA 428

BLAST of CcUC10G189910 vs. NCBI nr
Match: KAG6577532.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 400.6 bits (1028), Expect = 1.1e-107
Identity = 215/296 (72.64%), Postives = 232/296 (78.38%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P+VIS+FESKERQLHTTRSWDFLGLE QE VPSDSIW VTRFG++AIIANFDT    +  
Sbjct: 74  PNVISIFESKERQLHTTRSWDFLGLETQETVPSDSIWEVTRFGEDAIIANFDTGVWPESK 133

Query: 65  GLSLQGGSAVVKVTL-------TPTFTATGQEL--QFVPYLYFLPLLYFNSSRDHHGHGT 124
             S +G   +    L        P F    + +  +F  + Y      FNSSRDHHGHGT
Sbjct: 134 SFSDEGYGPIPTRWLGSCQSGADPNFRCNRKLIGARFFNFAYGPLNGSFNSSRDHHGHGT 193

Query: 125 QTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAAI 184
            TLSTAGGNFVSGANVFG GNGTAKGGSPRARVASYKVCW AE GGCFD DILAAFEAAI
Sbjct: 194 HTLSTAGGNFVSGANVFGYGNGTAKGGSPRARVASYKVCWEAEGGGCFDVDILAAFEAAI 253

Query: 185 SDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWMLT 244
            DGVDVIS SLG IPTDFLNDGLSIGAFHA+Q GIV+VCSAGNSGPAP      SPWMLT
Sbjct: 254 GDGVDVISTSLGGIPTDFLNDGLSIGAFHALQHGIVIVCSAGNSGPAPRTVSNVSPWMLT 313

Query: 245 VGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           VGASTIDR+FTNFVVLGNKK+ KGASLSSKAL V+KFYPLINAVD KANNV+  DA
Sbjct: 314 VGASTIDREFTNFVVLGNKKKLKGASLSSKALKVDKFYPLINAVDAKANNVSELDA 369

BLAST of CcUC10G189910 vs. NCBI nr
Match: XP_022965240.1 (subtilisin-like protease SBT5.3 [Cucurbita maxima])

HSP 1 Score: 388.3 bits (996), Expect = 5.8e-104
Identity = 214/296 (72.30%), Postives = 230/296 (77.70%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P+VISVFESKERQLHTTRSWDFLGLE QEAVPSDSIWNVTRFG++AIIAN DT    +  
Sbjct: 100 PNVISVFESKERQLHTTRSWDFLGLETQEAVPSDSIWNVTRFGEDAIIANIDTGVWPESR 159

Query: 65  GLSLQGGSAVVKVTL-------TPTFTATGQELQFVPY-LYFLPLL-YFNSSRDHHGHGT 124
             S +G   +    L        P F    + +    + L   PL   FNS RDH GHGT
Sbjct: 160 SFSDEGYGPIPSRWLGSCQSGADPNFHCNRKLIGARSFNLANGPLNGSFNSPRDHQGHGT 219

Query: 125 QTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAAI 184
            TLSTAGGNFVSGANVFG  NGT+KGGSPRARVASYKVCW AE GGCFD DILAAFEAAI
Sbjct: 220 HTLSTAGGNFVSGANVFGYANGTSKGGSPRARVASYKVCWEAEGGGCFDVDILAAFEAAI 279

Query: 185 SDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWMLT 244
            DGVDVIS SLGAIP+DFL+DGLSIGAFHAVQ GIVVVCSAGNSGPAP      SPWMLT
Sbjct: 280 GDGVDVISASLGAIPSDFLDDGLSIGAFHAVQHGIVVVCSAGNSGPAPRTVSNVSPWMLT 339

Query: 245 VGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           VGA TIDR+FTNFVVLGNKK+ KGASLSSKAL V+KFYPLINAVD KANNV+  DA
Sbjct: 340 VGAGTIDREFTNFVVLGNKKKLKGASLSSKALKVDKFYPLINAVDAKANNVSELDA 395

BLAST of CcUC10G189910 vs. NCBI nr
Match: XP_023553406.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.9 bits (951), Expect = 9.6e-99
Identity = 202/293 (68.94%), Postives = 220/293 (75.09%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P+VISVFE+KERQLHTTRSW+FLGLE QE VPSDSIWNVTRFG++AIIAN DT    +  
Sbjct: 100 PNVISVFENKERQLHTTRSWNFLGLETQEGVPSDSIWNVTRFGEDAIIANIDTGVWPESK 159

Query: 65  GLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYFLPLLYF-----------NSSRDHHGH 124
             S +G   V    L      +G +  F      +   +F           NS RDH GH
Sbjct: 160 SFSDEGYGPVPSRWLGS--CQSGADPNFHCNRKLIGARFFNLANRPLNGSSNSPRDHQGH 219

Query: 125 GTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEA 184
           GT TLSTAGGNFVSGANVFG  NGTAKGGSPRARVASYKVCW AE GGCFD DILAAFEA
Sbjct: 220 GTHTLSTAGGNFVSGANVFGYANGTAKGGSPRARVASYKVCWEAEGGGCFDVDILAAFEA 279

Query: 185 AISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWM 244
           AI DGVDV+S SLGAIP+DFL+DGL IGAFHAVQ GIVVVCSAGN GP P      SPWM
Sbjct: 280 AIGDGVDVVSASLGAIPSDFLDDGLCIGAFHAVQHGIVVVCSAGNLGPDPTSVSNVSPWM 339

Query: 245 LTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNV 280
           LTVGASTIDR+FTNF+VLGN K+ KGASLSSKAL  +KFYPLINAVD KANNV
Sbjct: 340 LTVGASTIDREFTNFIVLGNNKKLKGASLSSKALTEDKFYPLINAVDAKANNV 390

BLAST of CcUC10G189910 vs. NCBI nr
Match: KAG6577531.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 367.1 bits (941), Expect = 1.4e-97
Identity = 204/293 (69.62%), Postives = 219/293 (74.74%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P+VISVFE+KERQLHTTRSWDFLGLE Q  VPSDSIWNVTRFG +AIIAN DT    +  
Sbjct: 74  PNVISVFENKERQLHTTRSWDFLGLETQ-GVPSDSIWNVTRFGKDAIIANIDTGVWPESK 133

Query: 65  GLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYFLPLLYF-----------NSSRDHHGH 124
             S +G   V    L      +G +  F      +   +F           NS RDH GH
Sbjct: 134 SFSDEGYGPVPSRWLGS--CRSGADPNFHCNRKLIGARFFNLANGPLNGSLNSPRDHQGH 193

Query: 125 GTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEA 184
           GT TLSTAGGNFVSGANVFG  NGTAKGGSPRARVASYKVCW AE GGCFD DILAAFEA
Sbjct: 194 GTHTLSTAGGNFVSGANVFGYANGTAKGGSPRARVASYKVCWEAEGGGCFDVDILAAFEA 253

Query: 185 AISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWM 244
           AI DGVDVIS SLGAIP+DFL+DGLSIGAFHAVQ GIVVVCSAGN GP P      SPWM
Sbjct: 254 AIGDGVDVISASLGAIPSDFLDDGLSIGAFHAVQHGIVVVCSAGNLGPDPTSVSNVSPWM 313

Query: 245 LTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNV 280
           LTVGASTIDR+FTNF+VLGN K+ KGASLSSKAL  +KFYPLINAVD KANNV
Sbjct: 314 LTVGASTIDREFTNFIVLGNNKKLKGASLSSKALTDDKFYPLINAVDAKANNV 363

BLAST of CcUC10G189910 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.2e-75
Identity = 159/301 (52.82%), Postives = 198/301 (65.78%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT----- 64
           P V+SVF +K  +LHTTRSWDFLGLE    VPS SIW   RFG++ IIAN DT       
Sbjct: 102 PEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESK 161

Query: 65  --KDMGLS--LQGGSAVVKVTLTPTFTATGQEL--QFVPYLYFLPLLYFNSS----RDHH 124
             +D GL         + +     TF    + +  ++    Y   + + NSS    RD  
Sbjct: 162 SFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLD 221

Query: 125 GHGTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGG-CFDPDILAA 184
           GHG+ TLSTA G+FV G ++FG GNGTAKGGSPRARVA+YKVCWP   G  C+D D+LAA
Sbjct: 222 GHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAA 281

Query: 185 FEAAISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----S 244
           F+AAI DG DVISVSLG  PT F ND ++IG+FHA ++ IVVVCSAGNSGPA       +
Sbjct: 282 FDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVA 341

Query: 245 PWMLTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRD 285
           PW +TVGAST+DR+F + +VLGN K +KG SLSS ALP  KFYP++ +V+ KA N +  D
Sbjct: 342 PWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALD 401

BLAST of CcUC10G189910 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.4e-68
Identity = 149/298 (50.00%), Postives = 186/298 (62.42%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P V+SVF +K R+LHTT SW+F+ L +   V   S+WN   +G++ IIAN DT    +  
Sbjct: 112 PDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESK 171

Query: 65  GLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYF---------LPL-LYFNSSRDHHGHG 124
             S +G  AV                + +   YF         LP    + + RDH GHG
Sbjct: 172 SFSDEGYGAVPARWKGRCHKDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHG 231

Query: 125 TQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGG-CFDPDILAAFEA 184
           + TLSTA GNFV GANVFG GNGTA GGSP+ARVA+YKVCWP  DG  CFD DILAA EA
Sbjct: 232 SHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEA 291

Query: 185 AISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWM 244
           AI DGVDV+S S+G    D+++DG++IG+FHAV+ G+ VVCSAGNSGP  G     +PW+
Sbjct: 292 AIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWV 351

Query: 245 LTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           +TVGAS++DR+F  FV L N + FKG SL SK LP  K Y LI+A D    N    DA
Sbjct: 352 ITVGASSMDREFQAFVELKNGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDA 408

BLAST of CcUC10G189910 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 223.4 bits (568), Expect = 3.3e-57
Identity = 140/311 (45.02%), Postives = 180/311 (57.88%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P+V+SVF SKE +LHTTRSW+FLGL R+     +S W   RFG+  II N DT    +  
Sbjct: 102 PNVVSVFLSKEHKLHTTRSWEFLGLHRR---GQNSAWQKGRFGENTIIGNIDTGVWPESQ 161

Query: 65  GLSLQGGSAV--------VKVTLTPTFTATGQELQFVPYLYFL-----------PLLYFN 124
             S +G   V         ++   P         + +   Y+            PLL+  
Sbjct: 162 SFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLH-- 221

Query: 125 SSRDHHGHGTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAED-GGCFD 184
           ++RD  GHGT TLSTAGGNFV GA VF  GNGTAKGGSPRARVA+YKVCW   D   C+ 
Sbjct: 222 TARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYG 281

Query: 185 PDILAAFEAAISDGVDVISVSLG----AIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSG 244
            D+LAA + AI DGVDVI+VS G            D +SIGAFHA+ + I++V SAGN G
Sbjct: 282 ADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDG 341

Query: 245 PAPG-----SPWMLTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVD 285
           P PG     +PW+ T+ AST+DRDF++ + + N +  +GASL    LP N+ + LI + D
Sbjct: 342 PTPGTVANVAPWVFTIAASTLDRDFSSNLTI-NNQLIEGASLFVN-LPPNQAFSLILSTD 401

BLAST of CcUC10G189910 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.3e-49
Identity = 119/276 (43.12%), Postives = 161/276 (58.33%), Query Frame = 0

Query: 7   VISVFESKERQLHTTRSWDFLGL-----ERQEAVPSDSIWNVTRFGDEAIIANFDTVTKD 66
           V+SVF++ +RQLHTTRSWDFLGL     +R   + S+ I  V   G +    +F+    D
Sbjct: 101 VVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFN----D 160

Query: 67  MGLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYF----LPLLYFNSSRDHHGHGTQTLS 126
            G+         K      FT    ++    Y +     LP    +++ DH GHGT T S
Sbjct: 161 KGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSS 220

Query: 127 TAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAAISDGV 186
           T  G  VS A++FG  NGTA+GG P AR+A+YKVCW   D GC D D+LAAF+ AISDGV
Sbjct: 221 TIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCW---DSGCTDMDMLAAFDEAISDGV 280

Query: 187 DVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGP-----APGSPWMLTVGAS 246
           D+IS+S+G     F  D ++IGAFHA+++GI+  CSAGN+GP     +  +PW++TV A+
Sbjct: 281 DIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAAN 340

Query: 247 TIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPL 269
           ++DR F   V LGN     G SL+    P  K YPL
Sbjct: 341 SLDRKFETVVKLGNGLTASGISLNG-FNPRKKMYPL 368

BLAST of CcUC10G189910 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.0e-46
Identity = 122/292 (41.78%), Postives = 165/292 (56.51%), Query Frame = 0

Query: 1   MLGFPSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDT-VT 60
           M+    V+SV  ++ R+LHTT+SWDF+GL      P  +  ++    D  II   DT +T
Sbjct: 97  MMEMEEVVSVSRNQYRKLHTTKSWDFVGL------PLTAKRHLKAERD-VIIGVLDTGIT 156

Query: 61  KDMGLSLQGG----SAVVKVTLTPTFTATGQELQFVPYLYF-----LPLLYFNSSRDHHG 120
            D    L  G     A  K +  P    TG   + +   YF     +P     S  D  G
Sbjct: 157 PDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDG 216

Query: 121 HGTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFE 180
           HGT T ST  G  V+ A+++G  NGTA+G  P AR+A YKVCW     GC D DILA FE
Sbjct: 217 HGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWAR--SGCADMDILAGFE 276

Query: 181 AAISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPGS-----PW 240
           AAI DGV++IS+S+G    D+ +D +S+G+FHA+++GI+ V SAGN GP+ G+     PW
Sbjct: 277 AAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPW 336

Query: 241 MLTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKAN 278
           +LTV AS IDR F + + LGN K F G  +S  + P  K YPL++ VD   N
Sbjct: 337 ILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFS-PKAKSYPLVSGVDAAKN 378

BLAST of CcUC10G189910 vs. ExPASy TrEMBL
Match: A0A6J1HN63 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111465152 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.8e-104
Identity = 214/296 (72.30%), Postives = 230/296 (77.70%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P+VISVFESKERQLHTTRSWDFLGLE QEAVPSDSIWNVTRFG++AIIAN DT    +  
Sbjct: 100 PNVISVFESKERQLHTTRSWDFLGLETQEAVPSDSIWNVTRFGEDAIIANIDTGVWPESR 159

Query: 65  GLSLQGGSAVVKVTL-------TPTFTATGQELQFVPY-LYFLPLL-YFNSSRDHHGHGT 124
             S +G   +    L        P F    + +    + L   PL   FNS RDH GHGT
Sbjct: 160 SFSDEGYGPIPSRWLGSCQSGADPNFHCNRKLIGARSFNLANGPLNGSFNSPRDHQGHGT 219

Query: 125 QTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAAI 184
            TLSTAGGNFVSGANVFG  NGT+KGGSPRARVASYKVCW AE GGCFD DILAAFEAAI
Sbjct: 220 HTLSTAGGNFVSGANVFGYANGTSKGGSPRARVASYKVCWEAEGGGCFDVDILAAFEAAI 279

Query: 185 SDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWMLT 244
            DGVDVIS SLGAIP+DFL+DGLSIGAFHAVQ GIVVVCSAGNSGPAP      SPWMLT
Sbjct: 280 GDGVDVISASLGAIPSDFLDDGLSIGAFHAVQHGIVVVCSAGNSGPAPRTVSNVSPWMLT 339

Query: 245 VGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           VGA TIDR+FTNFVVLGNKK+ KGASLSSKAL V+KFYPLINAVD KANNV+  DA
Sbjct: 340 VGAGTIDREFTNFVVLGNKKKLKGASLSSKALKVDKFYPLINAVDAKANNVSELDA 395

BLAST of CcUC10G189910 vs. ExPASy TrEMBL
Match: A0A6J1EWY3 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111438777 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 6.7e-98
Identity = 204/293 (69.62%), Postives = 219/293 (74.74%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P+VISVFE+KERQLHTTRSWDFLGLE Q  VPSDSIWNVTRFG +AIIAN DT    +  
Sbjct: 100 PNVISVFENKERQLHTTRSWDFLGLETQ-GVPSDSIWNVTRFGKDAIIANIDTGVWPESK 159

Query: 65  GLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYFLPLLYF-----------NSSRDHHGH 124
             S +G   V    L      +G +  F      +   +F           NS RDH GH
Sbjct: 160 SFSDEGYGPVPSRWLGS--CRSGADPNFHCNRKLIGARFFNLANGPLNGSLNSPRDHQGH 219

Query: 125 GTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEA 184
           GT TLSTAGGNFVSGANVFG  NGTAKGGSPRARVASYKVCW AE GGCFD DILAAFEA
Sbjct: 220 GTHTLSTAGGNFVSGANVFGYANGTAKGGSPRARVASYKVCWEAEGGGCFDVDILAAFEA 279

Query: 185 AISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWM 244
           AI DGVDVIS SLGAIP+DFL+DGLSIGAFHAVQ GIVVVCSAGN GP P      SPWM
Sbjct: 280 AIGDGVDVISASLGAIPSDFLDDGLSIGAFHAVQHGIVVVCSAGNLGPDPTSVSNVSPWM 339

Query: 245 LTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNV 280
           LTVGASTIDR+FTNF+VLGN K+ KGASLSSKAL  +KFYPLINAVD KANNV
Sbjct: 340 LTVGASTIDREFTNFIVLGNNKKLKGASLSSKALTDDKFYPLINAVDAKANNV 389

BLAST of CcUC10G189910 vs. ExPASy TrEMBL
Match: A0A6J1G3J4 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111450435 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.7e-91
Identity = 183/297 (61.62%), Postives = 212/297 (71.38%), Query Frame = 0

Query: 4   FPSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KD 63
           FP+V+SVFE + R LHTTRSW+FLG+E+ E +P +SIWN  RFGD+ IIANFD+    + 
Sbjct: 13  FPNVVSVFECQARTLHTTRSWNFLGMEKHEGIPLNSIWNAARFGDDTIIANFDSGVWPEA 72

Query: 64  MGLSLQGGSAV-------VKVTLTPTFTATGQEL--QFVPYLYFLPLLYFNSSRDHHGHG 123
              S +G   +        +  L P F    + +  +F    Y +    FNS RD  GHG
Sbjct: 73  KSFSDEGYGPIPSRWKGTCQSDLDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG 132

Query: 124 TQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAA 183
           T TLS AGGNFVSGANVF   NGTAKGGSPRAR+ASYKVCWP E   C DP+ LAA++AA
Sbjct: 133 THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEGRQCLDPNALAAYDAA 192

Query: 184 ISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWML 243
           ISDGVDVISVS+G  P +FL D LS+GAFHAVQ GIVVVCSAGN GP P      SPW+L
Sbjct: 193 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 252

Query: 244 TVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           TVGASTIDRDFTNFVVLGNKK+ KG S SSKAL  NKFYPLINAVD KANN ++ DA
Sbjct: 253 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDA 309

BLAST of CcUC10G189910 vs. ExPASy TrEMBL
Match: A0A6J1KUM0 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498350 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 8.8e-90
Identity = 182/297 (61.28%), Postives = 209/297 (70.37%), Query Frame = 0

Query: 4   FPSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KD 63
           FP V+SVFE + R LHTTRSW+FLG+E+ E +PS SIWN+ RFG + IIANFD+    + 
Sbjct: 99  FPDVVSVFECQARTLHTTRSWNFLGMEKHEGIPSTSIWNIARFGGDTIIANFDSGVWPEA 158

Query: 64  MGLSLQGGSAV-------VKVTLTPTFTATGQEL--QFVPYLYFLPLLYFNSSRDHHGHG 123
              S +G   +        +    P F    + +  +F    Y      FNS RD  GHG
Sbjct: 159 KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGNLNATFNSPRDQEGHG 218

Query: 124 TQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAA 183
           T TLS AGGNFVSGANVF   NGTAKGGSPRAR+ASYKVCWP E   C DP+ LAA++AA
Sbjct: 219 THTLSIAGGNFVSGANVFDMANGTAKGGSPRARIASYKVCWPVEGRQCLDPNALAAYDAA 278

Query: 184 ISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWML 243
           ISDGVDVISVS+G  P +FL D LS+GAFHAVQ GIVVVCSAGN GP P      SPW+L
Sbjct: 279 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 338

Query: 244 TVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           TVGASTIDRDFTNFVVLGNKK+ KG S SSKAL  NKFYPLINAVD KANN ++ DA
Sbjct: 339 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDA 395

BLAST of CcUC10G189910 vs. ExPASy TrEMBL
Match: A0A5D3D737 (Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00080 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 5.7e-89
Identity = 183/298 (61.41%), Postives = 213/298 (71.48%), Query Frame = 0

Query: 4   FPSVISVFESKERQLHTTRSWDFLGLERQEAVP-SDSIWNVTRFGDEAIIANFDTVT--K 63
           FP V+SVFES+ R+LHTT+SW FLG+E+ E +P S+SIWNVTRFG++ IIANFDT    +
Sbjct: 80  FPHVVSVFESRSRKLHTTQSWKFLGVEKHEEIPTSNSIWNVTRFGEDIIIANFDTGVWPE 139

Query: 64  DMGLSLQGGSAV-------VKVTLTPTFTATGQEL--QFVPYLYFLPLLYFNSSRDHHGH 123
               S +G   +        +    P F    + +  +F    Y      FNSSRD+ GH
Sbjct: 140 SKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELADTFNSSRDNVGH 199

Query: 124 GTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEA 183
           GT TLS AGGNFV GANV G GNGT KGGSPRARVASYKVCWP E   C DP+ LAAFEA
Sbjct: 200 GTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANECMDPNTLAAFEA 259

Query: 184 AISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWM 243
           AI DGVDVIS+S+G  P +F +D LS+GAFHAV++GIVVV SAGN GP PG     SPW+
Sbjct: 260 AIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNMGPTPGTVSNVSPWI 319

Query: 244 LTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           LTVGAST DR FTNFV+LGNKK+FKG S SSK LPVNKFYPLINAVD KA NV+  DA
Sbjct: 320 LTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKAKNVSVSDA 377

BLAST of CcUC10G189910 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 284.6 bits (727), Expect = 8.4e-77
Identity = 159/301 (52.82%), Postives = 198/301 (65.78%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT----- 64
           P V+SVF +K  +LHTTRSWDFLGLE    VPS SIW   RFG++ IIAN DT       
Sbjct: 102 PEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESK 161

Query: 65  --KDMGLS--LQGGSAVVKVTLTPTFTATGQEL--QFVPYLYFLPLLYFNSS----RDHH 124
             +D GL         + +     TF    + +  ++    Y   + + NSS    RD  
Sbjct: 162 SFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLD 221

Query: 125 GHGTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGG-CFDPDILAA 184
           GHG+ TLSTA G+FV G ++FG GNGTAKGGSPRARVA+YKVCWP   G  C+D D+LAA
Sbjct: 222 GHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAA 281

Query: 185 FEAAISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----S 244
           F+AAI DG DVISVSLG  PT F ND ++IG+FHA ++ IVVVCSAGNSGPA       +
Sbjct: 282 FDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVA 341

Query: 245 PWMLTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRD 285
           PW +TVGAST+DR+F + +VLGN K +KG SLSS ALP  KFYP++ +V+ KA N +  D
Sbjct: 342 PWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALD 401

BLAST of CcUC10G189910 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.0e-69
Identity = 149/298 (50.00%), Postives = 186/298 (62.42%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDTVT--KDM 64
           P V+SVF +K R+LHTT SW+F+ L +   V   S+WN   +G++ IIAN DT    +  
Sbjct: 112 PDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESK 171

Query: 65  GLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYF---------LPL-LYFNSSRDHHGHG 124
             S +G  AV                + +   YF         LP    + + RDH GHG
Sbjct: 172 SFSDEGYGAVPARWKGRCHKDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHG 231

Query: 125 TQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGG-CFDPDILAAFEA 184
           + TLSTA GNFV GANVFG GNGTA GGSP+ARVA+YKVCWP  DG  CFD DILAA EA
Sbjct: 232 SHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEA 291

Query: 185 AISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPG-----SPWM 244
           AI DGVDV+S S+G    D+++DG++IG+FHAV+ G+ VVCSAGNSGP  G     +PW+
Sbjct: 292 AIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWV 351

Query: 245 LTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKANNVTNRDA 285
           +TVGAS++DR+F  FV L N + FKG SL SK LP  K Y LI+A D    N    DA
Sbjct: 352 ITVGASSMDREFQAFVELKNGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDA 408

BLAST of CcUC10G189910 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 196.4 bits (498), Expect = 3.0e-50
Identity = 119/276 (43.12%), Postives = 161/276 (58.33%), Query Frame = 0

Query: 7   VISVFESKERQLHTTRSWDFLGL-----ERQEAVPSDSIWNVTRFGDEAIIANFDTVTKD 66
           V+SVF++ +RQLHTTRSWDFLGL     +R   + S+ I  V   G +    +F+    D
Sbjct: 101 VVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFN----D 160

Query: 67  MGLSLQGGSAVVKVTLTPTFTATGQELQFVPYLYF----LPLLYFNSSRDHHGHGTQTLS 126
            G+         K      FT    ++    Y +     LP    +++ DH GHGT T S
Sbjct: 161 KGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSS 220

Query: 127 TAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFEAAISDGV 186
           T  G  VS A++FG  NGTA+GG P AR+A+YKVCW   D GC D D+LAAF+ AISDGV
Sbjct: 221 TIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCW---DSGCTDMDMLAAFDEAISDGV 280

Query: 187 DVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGP-----APGSPWMLTVGAS 246
           D+IS+S+G     F  D ++IGAFHA+++GI+  CSAGN+GP     +  +PW++TV A+
Sbjct: 281 DIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAAN 340

Query: 247 TIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPL 269
           ++DR F   V LGN     G SL+    P  K YPL
Sbjct: 341 SLDRKFETVVKLGNGLTASGISLNG-FNPRKKMYPL 368

BLAST of CcUC10G189910 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-47
Identity = 122/292 (41.78%), Postives = 165/292 (56.51%), Query Frame = 0

Query: 1   MLGFPSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDT-VT 60
           M+    V+SV  ++ R+LHTT+SWDF+GL      P  +  ++    D  II   DT +T
Sbjct: 97  MMEMEEVVSVSRNQYRKLHTTKSWDFVGL------PLTAKRHLKAERD-VIIGVLDTGIT 156

Query: 61  KDMGLSLQGG----SAVVKVTLTPTFTATGQELQFVPYLYF-----LPLLYFNSSRDHHG 120
            D    L  G     A  K +  P    TG   + +   YF     +P     S  D  G
Sbjct: 157 PDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDG 216

Query: 121 HGTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDPDILAAFE 180
           HGT T ST  G  V+ A+++G  NGTA+G  P AR+A YKVCW     GC D DILA FE
Sbjct: 217 HGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWAR--SGCADMDILAGFE 276

Query: 181 AAISDGVDVISVSLGAIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPAPGS-----PW 240
           AAI DGV++IS+S+G    D+ +D +S+G+FHA+++GI+ V SAGN GP+ G+     PW
Sbjct: 277 AAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPW 336

Query: 241 MLTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVNKFYPLINAVDVKAN 278
           +LTV AS IDR F + + LGN K F G  +S  + P  K YPL++ VD   N
Sbjct: 337 ILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFS-PKAKSYPLVSGVDAAKN 378

BLAST of CcUC10G189910 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 178.7 bits (452), Expect = 6.5e-45
Identity = 116/295 (39.32%), Postives = 168/295 (56.95%), Query Frame = 0

Query: 5   PSVISVFESKERQLHTTRSWDFLGLERQEAVPSDSIWNVTRFGDEAIIANFDT------- 64
           P+V++VFE + R+LHTTRS  FLGL+ Q+      +W+ + +G + II  FDT       
Sbjct: 85  PAVLAVFEDRRRELHTTRSPQFLGLQNQK-----GLWSESDYGSDVIIGVFDTGIWPERR 144

Query: 65  VTKDMGLS---------LQGGSAVV-----KVTLTPTFTATGQELQFVPYLYFLPLLYFN 124
              D+ L           + G+        +  +   F A GQ+   +  +     + F 
Sbjct: 145 SFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGIN--KTVEFL 204

Query: 125 SSRDHHGHGTQTLSTAGGNFVSGANVFGNGNGTAKGGSPRARVASYKVCWPAEDGGCFDP 184
           S RD  GHGT T STA G     A++ G  +G AKG +P+AR+A+YKVCW  +D GC D 
Sbjct: 205 SPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDS 264

Query: 185 DILAAFEAAISDGVDVISVSLG---AIPTDFLNDGLSIGAFHAVQQGIVVVCSAGNSGPA 244
           DILAAF+AA+ DGVDVIS+S+G    I + +  D ++IG++ A  +GI V  SAGN GP 
Sbjct: 265 DILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPN 324

Query: 245 PGS-----PWMLTVGASTIDRDFTNFVVLGNKKRFKGASLSSKALPVN-KFYPLI 270
             S     PW+ TVGASTIDR+F    +LG+  R +G SL +  +P+N + +P++
Sbjct: 325 GMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA-GVPLNGRMFPVV 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904160.13.0e-11677.44subtilisin-like protease SBT5.3 [Benincasa hispida][more]
KAG6577532.11.1e-10772.64Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022965240.15.8e-10472.30subtilisin-like protease SBT5.3 [Cucurbita maxima][more]
XP_023553406.19.6e-9968.94subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo][more]
KAG6577531.11.4e-9769.62Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9ZSP51.2e-7552.82Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
F4JXC51.4e-6850.00Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
I1N4623.3e-5745.02Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
Q9LZS64.3e-4943.12Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3... [more]
Q9LLL82.0e-4641.78Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1HN632.8e-10472.30subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111465152 PE=3... [more]
A0A6J1EWY36.7e-9869.62subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111438777 PE... [more]
A0A6J1G3J42.7e-9161.62subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111450435 PE... [more]
A0A6J1KUM08.8e-9061.28subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498350 PE=3... [more]
A0A5D3D7375.7e-8961.41Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
AT2G04160.18.4e-7752.82Subtilisin-like serine endopeptidase family protein [more]
AT5G59810.11.0e-6950.00Subtilase family protein [more]
AT5G03620.13.0e-5043.12Subtilisin-like serine endopeptidase family protein [more]
AT4G00230.11.4e-4741.78xylem serine peptidase 1 [more]
AT4G34980.16.5e-4539.32subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 102..234
e-value: 4.1E-66
score: 225.7
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 18..270
NoneNo IPR availableGENE3D3.50.30.30coord: 235..281
e-value: 4.1E-66
score: 225.7
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 5..59
coord: 103..281
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 23..284
score: 15.983799
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 103..220
e-value: 7.3E-15
score: 55.0
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 5..59
coord: 103..281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC10G189910.1CcUC10G189910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity