CcUC09G168660 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC09G168660
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionThiamine pyrophosphokinase
LocationCicolChr09: 4983339 .. 4986782 (-)
RNA-Seq ExpressionCcUC09G168660
SyntenyCcUC09G168660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGTCTTGTTTCACTCTTCGGAATTCCTCCTCCCTTCTTCTTCTTCTTCTCCGCCCGACCACCGCCGTTCTTTATCCTACGCCCTTGTTCTGCTCAATCAACGCCTCCCCAAATTCACTCCCCTGCTCTGGAAGCACGGTTAATCTACGATTCCCTTTTCCTCCGTTTTTTGGTTCTGGGTTTTGATTTTTTAGGGGTTTTTCGAGGGTTTTGATGGATTTCTTGTGTTGATATGTTTTTATGTGTGTTGTTGTAGAGCAGCACAACTTCGTTTGTGTGCCGATGGAGGCGCCAATCGTGTATTTGATGAATTGCCTCTGATGTTACCTCATCTAGACGCTTTGGATGTTCGTAACAGGTTTGCTAAGTGTCTTTTTCTTCCTCTTTCTTATAGCATGCATTTCTTCCATTATTAGTTTCTTTTTAATGTGATTTAGTTTCTCTCTTTAGTAATGGAGTTTTGTGCCTAGGGGTTTCGTTATCTGTTTGTTTTGGAAATTTAGATGGCTAATGGGTAGAAACCCTTTTGGAGAGACAGATTATTGATGATATGGACCATGCCCTGGATATTACTGTACAGTTTTAGTTACTACCCACTGCTGTGTTATGATGAACTTTTGCCCTCCCTTTGGAGTCAACAAAACTTTAATGATTTTAATCTCGTAATTTGAAATCCTAGAAATGATTTTAAACTTTTTACTTTGATTGGAATGAGAAAATTTTCAAATGAAATAAGAAATTATTTCATCGATAGAAAGAAAGATAAGAAGTTACAAATATGGGCTTTTAATTATGATTTGTTAAATGGAATTATAATATGACAAGGTTAAATAAGTAATTTACCTCTAAATCCGATGAAATCATGCAAAAACCGTCTATTTTAGTCAAGAAACTTTTTTCACAAAAATGGAATTTGAAATCAACAAACTACAAGAATCGTTTGAAATCCCTCAAAATCATGAGGTTCCCTTGAAATTTACCTTAGGGTTCATTATTAATTTTTTTTTGGTTATATTTTCATTTATTTCAAATGTCAACTGTTTCTTATTATGTTACATATTTTAAAGTTTTTCTTTTTCTTATTTCGGTCAATTCTTTCTTTACTGTTATTGGATTTTTCTTTCTTTATTAATTATTAATCATGGAATTGTTAATGATTGTGTGTAATCTTAACGTCTACAAATCACCCAATCTCGATCCTTAAAAGTAATATTCTTGCTGGAAAGGAAGAAACTACCCAATATGTTTCAAGTTTGAATATGCGATTGATACCAAATGAAACAAATGCCAATGGCTGGCTGGTTGAAGAAGGTATTTAAAGCTATTTTGTTGGCTTTTGATGTCTACATATCATTCTCGGGCATTTCATGATAGTTGATATTATTATTTTAAAATGAATGAACTATTCTAGAATTATATATTTTATGCTTTGATGACCTTCAATGTACTTTGATCCTATCTATTGGATTTTTTTGTTTTATTTTTTAATTGCCAGCTACAAGCCAGATGTTATTAGAGGAGACATGGATTCAATTCGGACAGAAGTACTAGAATTTTATGCTAAGCAAGTATGTCATTCTGACCTTTCCCTTGAAAGAGTTCAGAATTCTATAATAACCGTTTGGTAATTTATTTTTTGCTTTTGAAAATTGTGTGCTTTGCTTCTACCGATTTCTTTACTATAATTTTCATCCTTCCTTAGGAAACATTTGAATTCTCAATCAAACTTAAAAAAAAAAAAAAAAACAAGTTTTGAAAAACTATTTATATATTTTTTTCAAATTTTTGCTTAAAGAAATCCATAGGCAGAAATAGGGTTCATAAGCTTAATTCTCAAAACCAAAACCAGATATCAAGTAGTTATCAAATGCATCTTTAGCTTTTGCCATCTATTTTGAGACCATGGATAGTTCTCATTCTGTACATACCGGACTAACCCACTGGTCCTTTTATGCATCTTATGTGTAATTTTTCCCTTTTTATGTGTTGATGAAATTCACTGGCATTTCATGTTATCATGTATACATTTCTGGCTCAAGATATTCACACGATGCAGGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATCAGGAGGAGTCTGATGTAGGTTGAATCATGATGTAAATCTTAAGTAGCTTGTTGATTAAGCTAGGACTTCATTTTACTATTTACATTCAATGATTCAATTACTGCACTTGTTTCTCTGCTCTGACTTTTCAGATGGCATTATAATGTTGAGCTGCTCATTTTCAGCTATGTATACTTGTTGCTGGAGCATTAGGTGGACGTTTTGATCATGAGATAGGAAACATCAATGTTCTATGCCGTTTCTCAACCACGCGCATAATCCTTCTGTCTGATGATTGTCTTATTCATCTTCTCCCAAGGACTCATCACCATGAAATCCATGTTCGCTCATCTGTTGAGGGTCCACATTGTGGTCTCATTCCAATTGGGATGCCTTCTGGAAGCACTACAACCACTGGTCTCCAATGGGATTTGAGTAAGTACTGTCAATATCCTACTTTCAAATTGTTCTCCTCCCATCTTCTGGATCTAATGCTGTTTTTTGTTCTCAATGAATGGCTGGCTGTGTCATGTTTTAAGCAACTTTGAGATGCAAATTAGCTAACATAATATGATCCATCAGTTTTATTCCTCTTGCTATGAATTCATTCTCAATGAATGGCTGGCTGTGTCATGTTTTAAGCAACTTTGAGCTGCAAATTAGCCAACATAATATGATCCATCAGTTTTATTCCTCTTGCTATGAATTCATTCTTAACTTTATTTTTCGAAAACAACTTCATTGAAAACACGCAAGGAATAGGTCAATTCAACAGCCTGTCACTTAGTTTCCTTAGATTTTCCATAAACTCCAGAACAAACAACAAATGTGGGGAATGGAGATTTGAACCTCTAACATCAAGAGACGGAGAAGTCAATATCTCATTGATGATTTCTTTCCTGATAGATAATGATTACATCACTCTCTCCCTATGTGATACTACTTTGGCATGATATTCCATTGAATTGTGACTTACAAGATAAGGAAATGTTTTGGATCTAAGTTAGGGATGTTCTCAAATCTGCCTCAATTAACTGCAAATTTAGGACACTCTTTTCTTTTTCAATTGGAACTCTCTGCTCTGTCTGAACTGTTTATTCCCAATTGGGAAGTTTTTTTTTTTTTTTTTGTAATGGAATACTTTGCAATTTAATGCCTGTTGTTAACTTTTTGGTATTATTAATGTGTATTCCAGATGACACAGAGATGAAGTTTGGAGGTCTGATTAGCACATCAAATATAGTTAAAGAAGAGAAAGTAACAGTGCAATCTGACTCCGATCTTCTCTGGACGATCTCATTAAAAAGCCAAATTTAG

mRNA sequence

ATGGGCGTCTTGTTTCACTCTTCGGAATTCCTCCTCCCTTCTTCTTCTTCTTCTCCGCCCGACCACCGCCGTTCTTTATCCTACGCCCTTGTTCTGCTCAATCAACGCCTCCCCAAATTCACTCCCCTGCTCTGGAAGCACGCACAACTTCGTTTGTGTGCCGATGGAGGCGCCAATCGTGTATTTGATGAATTGCCTCTGATGTTACCTCATCTAGACGCTTTGGATGTTCGTAACAGCTACAAGCCAGATGTTATTAGAGGAGACATGGATTCAATTCGGACAGAAGTACTAGAATTTTATGCTAAGCAAGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATCAGGAGGAGTCTGATCTATGTATACTTGTTGCTGGAGCATTAGGTGGACGTTTTGATCATGAGATAGGAAACATCAATGTTCTATGCCGTTTCTCAACCACGCGCATAATCCTTCTGTCTGATGATTGTCTTATTCATCTTCTCCCAAGGACTCATCACCATGAAATCCATGTTCGCTCATCTGTTGAGGGTCCACATTGTGGTCTCATTCCAATTGGGATGCCTTCTGGAAGCACTACAACCACTGGTCTCCAATGGGATTTGAATGACACAGAGATGAAGTTTGGAGGTCTGATTAGCACATCAAATATAGTTAAAGAAGAGAAAGTAACAGTGCAATCTGACTCCGATCTTCTCTGGACGATCTCATTAAAAAGCCAAATTTAG

Coding sequence (CDS)

ATGGGCGTCTTGTTTCACTCTTCGGAATTCCTCCTCCCTTCTTCTTCTTCTTCTCCGCCCGACCACCGCCGTTCTTTATCCTACGCCCTTGTTCTGCTCAATCAACGCCTCCCCAAATTCACTCCCCTGCTCTGGAAGCACGCACAACTTCGTTTGTGTGCCGATGGAGGCGCCAATCGTGTATTTGATGAATTGCCTCTGATGTTACCTCATCTAGACGCTTTGGATGTTCGTAACAGCTACAAGCCAGATGTTATTAGAGGAGACATGGATTCAATTCGGACAGAAGTACTAGAATTTTATGCTAAGCAAGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATCAGGAGGAGTCTGATCTATGTATACTTGTTGCTGGAGCATTAGGTGGACGTTTTGATCATGAGATAGGAAACATCAATGTTCTATGCCGTTTCTCAACCACGCGCATAATCCTTCTGTCTGATGATTGTCTTATTCATCTTCTCCCAAGGACTCATCACCATGAAATCCATGTTCGCTCATCTGTTGAGGGTCCACATTGTGGTCTCATTCCAATTGGGATGCCTTCTGGAAGCACTACAACCACTGGTCTCCAATGGGATTTGAATGACACAGAGATGAAGTTTGGAGGTCTGATTAGCACATCAAATATAGTTAAAGAAGAGAAAGTAACAGTGCAATCTGACTCCGATCTTCTCTGGACGATCTCATTAAAAAGCCAAATTTAG

Protein sequence

MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANRVFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDLHKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHLLPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKEEKVTVQSDSDLLWTISLKSQI
Homology
BLAST of CcUC09G168660 vs. NCBI nr
Match: XP_008451592.1 (PREDICTED: thiamine pyrophosphokinase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 499.2 bits (1284), Expect = 2.2e-137
Identity = 245/260 (94.23%), Postives = 253/260 (97.31%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M VLFHSS+FLLPS SSSPP+HRRS +YALVLLNQRLPKFTPLLWKHAQLRLCADGGANR
Sbjct: 1   MEVLFHSSDFLLPSPSSSPPNHRRSSTYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           VFD+LPLM PHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDDLPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQS+PNQ ES+LCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSIPNQ-ESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LPRTHHHEI + SSVEGPHCGLIPIGMPSGSTTTTGLQWDL+DTEMKFGGLISTSNIVKE
Sbjct: 181 LPRTHHHEILIHSSVEGPHCGLIPIGMPSGSTTTTGLQWDLSDTEMKFGGLISTSNIVKE 240

Query: 241 EKVTVQSDSDLLWTISLKSQ 261
           EKVTVQSDSDLLWTISLKSQ
Sbjct: 241 EKVTVQSDSDLLWTISLKSQ 259

BLAST of CcUC09G168660 vs. NCBI nr
Match: XP_038899470.1 (thiamine pyrophosphokinase 1 [Benincasa hispida] >XP_038899471.1 thiamine pyrophosphokinase 1 [Benincasa hispida])

HSP 1 Score: 491.9 bits (1265), Expect = 3.4e-135
Identity = 244/259 (94.21%), Postives = 248/259 (95.75%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           MGVLFHSSEFLLPSSS   PDHR S +Y LVLLNQRLPKFTPLLWK AQLRLCADGGANR
Sbjct: 1   MGVLFHSSEFLLPSSSR--PDHRHSSTYVLVLLNQRLPKFTPLLWKAAQLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLM PHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPN EES+L ILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNLEESNLYILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LPRTHHHEI+V SSVEGPHCGLIPIGMPSGSTTTTGLQWDL+DTEMKFGGLISTSNIVKE
Sbjct: 181 LPRTHHHEIYVHSSVEGPHCGLIPIGMPSGSTTTTGLQWDLSDTEMKFGGLISTSNIVKE 240

Query: 241 EKVTVQSDSDLLWTISLKS 260
           EKVTVQSDSDLLWTISLKS
Sbjct: 241 EKVTVQSDSDLLWTISLKS 257

BLAST of CcUC09G168660 vs. NCBI nr
Match: XP_023515266.1 (thiamine pyrophosphokinase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.1 bits (1250), Expect = 1.9e-133
Identity = 234/258 (90.70%), Postives = 245/258 (94.96%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           MGV FHSS+FLLPSS+SSP  H  S +YALVLLNQ LPKFTPLLWKHA+LRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           VFDELPL+LPH DA D+RNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLLPHQDATDIRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEES+LCILVAGALGGRFDHE+GN+NVLCRFS+TRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNVNVLCRFSSTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LPRTHHHEIHV SSVEGPHCGLIPIGMPSG TTTTGLQWDL DTEMKFGGL+STSNIVK 
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTISLK 259
           EKVTVQSDSDLLWTISLK
Sbjct: 241 EKVTVQSDSDLLWTISLK 258

BLAST of CcUC09G168660 vs. NCBI nr
Match: XP_011659355.1 (thiamine pyrophosphokinase 1 isoform X1 [Cucumis sativus] >KGN44926.1 hypothetical protein Csa_015786 [Cucumis sativus])

HSP 1 Score: 485.0 bits (1247), Expect = 4.2e-133
Identity = 243/263 (92.40%), Postives = 249/263 (94.68%), Query Frame = 0

Query: 1   MGVLFHSSEFLLP----SSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADG 60
           M VLFHSS+FLLP    SSSSS PD RRS +YALVLLNQRLPKFTPLLWKHAQLRLCADG
Sbjct: 1   MEVLFHSSDFLLPSPSSSSSSSLPDRRRSSTYALVLLNQRLPKFTPLLWKHAQLRLCADG 60

Query: 61  GANRVFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQD 120
           GANRVFDE PLM PHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYA QGTKIFDESEDQD
Sbjct: 61  GANRVFDEFPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAMQGTKIFDESEDQD 120

Query: 121 TTDLHKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDC 180
           TTDLHKCVAYILQS+PNQ ES+LCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDC
Sbjct: 121 TTDLHKCVAYILQSIPNQ-ESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDC 180

Query: 181 LIHLLPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSN 240
           LIHLLPRTHHHEI V SSVEGPHCGLIPIGMPSGSTTTTGL+WDL+DTEMKFGGLISTSN
Sbjct: 181 LIHLLPRTHHHEILVHSSVEGPHCGLIPIGMPSGSTTTTGLEWDLSDTEMKFGGLISTSN 240

Query: 241 IVKEEKVTVQSDSDLLWTISLKS 260
           IVKEEKVTVQSDSDLLWTISLKS
Sbjct: 241 IVKEEKVTVQSDSDLLWTISLKS 262

BLAST of CcUC09G168660 vs. NCBI nr
Match: KAG7030224.1 (Thiamine pyrophosphokinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 484.6 bits (1246), Expect = 5.5e-133
Identity = 233/258 (90.31%), Postives = 244/258 (94.57%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           MGV FHSS+FLLPSS+SSP  H  S +YALVLLNQ LPKFTPLLWKHA+LRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           VFDELPL+ PH DA D+RNSYKPDVIRGDMDSIRTEVL+FYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEES+LCILVAGALGGRFDHE+GNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LPRTHHHEIHV SSVEGPHCGLIPIGMPSG TTTTGLQWDL DTEMKFGGL+STSNIVK 
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTISLK 259
           EKVTVQSDSDLLWT+SLK
Sbjct: 241 EKVTVQSDSDLLWTLSLK 258

BLAST of CcUC09G168660 vs. ExPASy Swiss-Prot
Match: B9DGU7 (Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 2.5e-104
Identity = 181/258 (70.16%), Postives = 211/258 (81.78%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M V+ HSS FLLP   +S         YALV+LNQ LP+FTPLLW+HA+LRLCADGGANR
Sbjct: 4   MDVMIHSSSFLLPCDETS-----TGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANR 63

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL  P+ DAL +RN YKPDVI+GDMDSIR +VL+FY   GTK+ DES DQDTTDL
Sbjct: 64  IYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDL 123

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
            KC+ YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 124 DKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 183

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LP+TH HEIH++SS+EGPHCGLIPIG PS  TTT+GLQWDL++TEM+FGGLISTSN+VKE
Sbjct: 184 LPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKE 243

Query: 241 EKVTVQSDSDLLWTISLK 259
           EK+TV+SDSDLLWTIS+K
Sbjct: 244 EKITVESDSDLLWTISIK 256

BLAST of CcUC09G168660 vs. ExPASy Swiss-Prot
Match: F4IV16 (Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.3e-102
Identity = 177/258 (68.60%), Postives = 208/258 (80.62%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M V+ HSS FLLP       D      YALV+LNQ LP+FTPLLW+HA+LRLCADGGANR
Sbjct: 5   MDVMIHSSSFLLPC------DETCGTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANR 64

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL  PH D   +RN YKPDVI+GDMDSIR +VL+FY   GTK+ DES DQDTTDL
Sbjct: 65  IYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDL 124

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
            KC++YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 125 DKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 184

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LP+TH HEIH+ SS++GPHCGLIPIG PS +TTT+GL+WDL++TEM+FGGLISTSN+VKE
Sbjct: 185 LPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKE 244

Query: 241 EKVTVQSDSDLLWTISLK 259
           E +TV+SDSDLLWTIS+K
Sbjct: 245 EIITVESDSDLLWTISIK 256

BLAST of CcUC09G168660 vs. ExPASy Swiss-Prot
Match: Q5JK24 (Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 7.3e-96
Identity = 172/257 (66.93%), Postives = 207/257 (80.54%), Query Frame = 0

Query: 6   HSSEFL-LPSSSS--SPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANRVF 65
           HSS FL LP++SS   PP    S +YA+V+LNQRLP+F PLLW  A+LR+CADGGANRVF
Sbjct: 8   HSSSFLRLPATSSPHPPPADDASAAYAVVVLNQRLPRFAPLLWDRARLRVCADGGANRVF 67

Query: 66  DELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDLHK 125
           D +P +LP  D   VR  YKPDVI+GDMDSIR EV E+Y+  G +I DES DQDTTDLHK
Sbjct: 68  DGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQDTTDLHK 127

Query: 126 CVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHLLP 185
           CV++I ++ P  EES+L ILV GALGGRFDHE+GNINVL RFS  RI+LLSDDC I LLP
Sbjct: 128 CVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDDCSIFLLP 187

Query: 186 RTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKEEK 245
           +TH HEIH+  S+EGPHCGLIP+G PS STTTTGL+W+L++T M +GGLISTSNIV+EE 
Sbjct: 188 KTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTSNIVEEET 247

Query: 246 VTVQSDSDLLWTISLKS 260
           V + SDSDL+WTISL++
Sbjct: 248 VRITSDSDLIWTISLRN 264

BLAST of CcUC09G168660 vs. ExPASy Swiss-Prot
Match: Q60DX1 (Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.3e-92
Identity = 166/256 (64.84%), Postives = 200/256 (78.12%), Query Frame = 0

Query: 6   HSSEFLLPS-SSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANRVFDE 65
           HSS FLLPS S+++        +YAL++LNQRLP+F P LW  AQ+R+CADGGANRVFD 
Sbjct: 9   HSSAFLLPSPSAAAAGADADGAAYALLVLNQRLPRFAPRLWDRAQVRVCADGGANRVFDG 68

Query: 66  LPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDLHKCV 125
           +P + P  D  +VR  YKPDVI+GD+DS+R EV E+Y+  GT+I DES DQDTTDLHKCV
Sbjct: 69  MPELFPGQDPDEVRRRYKPDVIKGDLDSVRPEVKEYYSNMGTQIVDESHDQDTTDLHKCV 128

Query: 126 AYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHLLPRT 185
           A+I ++     +S+LCI   GALGGRFDHE+GNINVL  F   RIILLSDDCLI LLPRT
Sbjct: 129 AFITENSAIPNKSNLCIFALGALGGRFDHEMGNINVLHLFPNNRIILLSDDCLIFLLPRT 188

Query: 186 HHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKEEK-- 245
           H H IH+  S+EGPHCGLIPIG PS +TTTTGLQW+L++T M FGGLISTSNIV+EE   
Sbjct: 189 HTHNIHIERSIEGPHCGLIPIGAPSATTTTTGLQWNLDNTSMSFGGLISTSNIVREESTV 248

Query: 246 VTVQSDSDLLWTISLK 259
           VT+ SDSDL+WTISL+
Sbjct: 249 VTITSDSDLIWTISLR 264

BLAST of CcUC09G168660 vs. ExPASy Swiss-Prot
Match: Q0JMW0 (Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.4e-91
Identity = 160/259 (61.78%), Postives = 201/259 (77.61%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M V+ HSS FLLP      P +  +  YALV+LNQ LP+F P LW HA+LR+CADGGANR
Sbjct: 9   MEVMVHSSTFLLPKLHQ--PANSPAKKYALVVLNQNLPRFVPRLWTHAKLRICADGGANR 68

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           +FDE+  M    D    R  Y P++I GDMDSIR EV +FY+ QG+KI D+S +Q+TTDL
Sbjct: 69  IFDEMFQMTNDPDYESTRKRYIPEIIEGDMDSIRPEVKQFYSSQGSKISDKSHNQETTDL 128

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKC++ I +  P+ E+++LC+LV GALGGRFDHE  NIN+L  FS  RI+LLSDDCLI L
Sbjct: 129 HKCISRIHRCTPDHEKTNLCVLVTGALGGRFDHEAANINILYLFSDMRIVLLSDDCLIRL 188

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LP+TH HEI++ SSVEGPHCGL P+G PSGSTTTTGL+W+L++ +M+FG +ISTSNIV  
Sbjct: 189 LPKTHKHEIYIESSVEGPHCGLFPVGAPSGSTTTTGLKWNLSEAKMRFGSMISTSNIVHA 248

Query: 241 EKVTVQSDSDLLWTISLKS 260
           EKVTVQSD+DLLWTISL++
Sbjct: 249 EKVTVQSDADLLWTISLRN 265

BLAST of CcUC09G168660 vs. ExPASy TrEMBL
Match: A0A1S3BSZ4 (Thiamine pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103492826 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.0e-137
Identity = 245/260 (94.23%), Postives = 253/260 (97.31%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M VLFHSS+FLLPS SSSPP+HRRS +YALVLLNQRLPKFTPLLWKHAQLRLCADGGANR
Sbjct: 1   MEVLFHSSDFLLPSPSSSPPNHRRSSTYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           VFD+LPLM PHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDDLPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQS+PNQ ES+LCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSIPNQ-ESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LPRTHHHEI + SSVEGPHCGLIPIGMPSGSTTTTGLQWDL+DTEMKFGGLISTSNIVKE
Sbjct: 181 LPRTHHHEILIHSSVEGPHCGLIPIGMPSGSTTTTGLQWDLSDTEMKFGGLISTSNIVKE 240

Query: 241 EKVTVQSDSDLLWTISLKSQ 261
           EKVTVQSDSDLLWTISLKSQ
Sbjct: 241 EKVTVQSDSDLLWTISLKSQ 259

BLAST of CcUC09G168660 vs. ExPASy TrEMBL
Match: A0A0A0KAV4 (Thiamine pyrophosphokinase OS=Cucumis sativus OX=3659 GN=Csa_7G396410 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.0e-133
Identity = 243/263 (92.40%), Postives = 249/263 (94.68%), Query Frame = 0

Query: 1   MGVLFHSSEFLLP----SSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADG 60
           M VLFHSS+FLLP    SSSSS PD RRS +YALVLLNQRLPKFTPLLWKHAQLRLCADG
Sbjct: 1   MEVLFHSSDFLLPSPSSSSSSSLPDRRRSSTYALVLLNQRLPKFTPLLWKHAQLRLCADG 60

Query: 61  GANRVFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQD 120
           GANRVFDE PLM PHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYA QGTKIFDESEDQD
Sbjct: 61  GANRVFDEFPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAMQGTKIFDESEDQD 120

Query: 121 TTDLHKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDC 180
           TTDLHKCVAYILQS+PNQ ES+LCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDC
Sbjct: 121 TTDLHKCVAYILQSIPNQ-ESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDC 180

Query: 181 LIHLLPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSN 240
           LIHLLPRTHHHEI V SSVEGPHCGLIPIGMPSGSTTTTGL+WDL+DTEMKFGGLISTSN
Sbjct: 181 LIHLLPRTHHHEILVHSSVEGPHCGLIPIGMPSGSTTTTGLEWDLSDTEMKFGGLISTSN 240

Query: 241 IVKEEKVTVQSDSDLLWTISLKS 260
           IVKEEKVTVQSDSDLLWTISLKS
Sbjct: 241 IVKEEKVTVQSDSDLLWTISLKS 262

BLAST of CcUC09G168660 vs. ExPASy TrEMBL
Match: A0A6J1KV43 (Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111497866 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.7e-133
Identity = 233/258 (90.31%), Postives = 245/258 (94.96%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           MGV FHSS+FLLPSS+SSP  H  S +YALVLLNQRLPKFTPLLWKHA+LRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQRLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           VFDELPL+ PH DA D+RNSYKPDVIRGDMDSIRTEVL+FYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEES+LCILVAGALGGRFDHE+GNINVLCRFS+TRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LPRTHHHEIHV SSVEGPHCGLIPIGMPSG TTTTGLQWDL D+EMKFGGL+STSNIVK 
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDSEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTISLK 259
           EKVTVQSDSDLLWTISLK
Sbjct: 241 EKVTVQSDSDLLWTISLK 258

BLAST of CcUC09G168660 vs. ExPASy TrEMBL
Match: A0A6J1GPJ2 (Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 6.5e-132
Identity = 232/260 (89.23%), Postives = 244/260 (93.85%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M VLFHSSEFLLPSSSS P +HRRS +YALVLLNQRLPKFTPLLWKHAQLRLCADGGANR
Sbjct: 1   MSVLFHSSEFLLPSSSSFPAEHRRSSTYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           +FDELPL+ PHLDALDVRNSYKPDVI GDMDSIRTEVL+FYAKQGTKIFDESED+DTTDL
Sbjct: 61  IFDELPLLFPHLDALDVRNSYKPDVISGDMDSIRTEVLDFYAKQGTKIFDESEDEDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPN EES+LCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCL+HL
Sbjct: 121 HKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LPRTHHHEIHV SSVEGPHCGLIPIGMPSGSTTT GLQWDL DT+ KFGGLIS+SN VK 
Sbjct: 181 LPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKG 240

Query: 241 EKVTVQSDSDLLWTISLKSQ 261
           EKV V SD+DLLWTISLK +
Sbjct: 241 EKVRVLSDTDLLWTISLKKE 260

BLAST of CcUC09G168660 vs. ExPASy TrEMBL
Match: A0A6J1JRW1 (Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111488361 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.5e-131
Identity = 234/263 (88.97%), Postives = 244/263 (92.78%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSS---PPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGG 60
           M VLFHSSEFLLPSSSSS   P +HRRS +YALVLLNQRLPKFTPLLWKH+QLRLCADGG
Sbjct: 1   MSVLFHSSEFLLPSSSSSSSFPAEHRRSSTYALVLLNQRLPKFTPLLWKHSQLRLCADGG 60

Query: 61  ANRVFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDT 120
           ANRVFDELPL+ PHLDALDVRNSYKPDVI GDMDSIR EVL+FY KQG KIFDESED+DT
Sbjct: 61  ANRVFDELPLLFPHLDALDVRNSYKPDVISGDMDSIRAEVLDFYDKQGIKIFDESEDEDT 120

Query: 121 TDLHKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCL 180
           TDLHKCVAYILQSMPN EES+LCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCL
Sbjct: 121 TDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCL 180

Query: 181 IHLLPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNI 240
           +HLLPRTHHHEIHV SSVEGPHCGLIPIGMPSGSTTT GLQWDL DTEMKFGGLIS+SN 
Sbjct: 181 VHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTKGLQWDLTDTEMKFGGLISSSNK 240

Query: 241 VKEEKVTVQSDSDLLWTISLKSQ 261
           VK EKV VQSD+DLLWTISLK Q
Sbjct: 241 VKGEKVRVQSDTDLLWTISLKKQ 263

BLAST of CcUC09G168660 vs. TAIR 10
Match: AT1G02880.2 (thiamin pyrophosphokinase1 )

HSP 1 Score: 379.8 bits (974), Expect = 1.8e-105
Identity = 181/258 (70.16%), Postives = 211/258 (81.78%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M V+ HSS FLLP   +S         YALV+LNQ LP+FTPLLW+HA+LRLCADGGANR
Sbjct: 1   MDVMIHSSSFLLPCDETS-----TGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANR 60

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL  P+ DAL +RN YKPDVI+GDMDSIR +VL+FY   GTK+ DES DQDTTDL
Sbjct: 61  IYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
            KC+ YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 121 DKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 180

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LP+TH HEIH++SS+EGPHCGLIPIG PS  TTT+GLQWDL++TEM+FGGLISTSN+VKE
Sbjct: 181 LPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKE 240

Query: 241 EKVTVQSDSDLLWTISLK 259
           EK+TV+SDSDLLWTIS+K
Sbjct: 241 EKITVESDSDLLWTISIK 253

BLAST of CcUC09G168660 vs. TAIR 10
Match: AT1G02880.3 (thiamin pyrophosphokinase1 )

HSP 1 Score: 379.8 bits (974), Expect = 1.8e-105
Identity = 181/258 (70.16%), Postives = 211/258 (81.78%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M V+ HSS FLLP   +S         YALV+LNQ LP+FTPLLW+HA+LRLCADGGANR
Sbjct: 4   MDVMIHSSSFLLPCDETS-----TGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANR 63

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL  P+ DAL +RN YKPDVI+GDMDSIR +VL+FY   GTK+ DES DQDTTDL
Sbjct: 64  IYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDL 123

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
            KC+ YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 124 DKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 183

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LP+TH HEIH++SS+EGPHCGLIPIG PS  TTT+GLQWDL++TEM+FGGLISTSN+VKE
Sbjct: 184 LPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKE 243

Query: 241 EKVTVQSDSDLLWTISLK 259
           EK+TV+SDSDLLWTIS+K
Sbjct: 244 EKITVESDSDLLWTISIK 256

BLAST of CcUC09G168660 vs. TAIR 10
Match: AT2G44750.2 (thiamin pyrophosphokinase 2 )

HSP 1 Score: 373.2 bits (957), Expect = 1.7e-103
Identity = 177/258 (68.60%), Postives = 208/258 (80.62%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M V+ HSS FLLP       D      YALV+LNQ LP+FTPLLW+HA+LRLCADGGANR
Sbjct: 5   MDVMIHSSSFLLPC------DETCGTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANR 64

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL  PH D   +RN YKPDVI+GDMDSIR +VL+FY   GTK+ DES DQDTTDL
Sbjct: 65  IYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDL 124

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
            KC++YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 125 DKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 184

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LP+TH HEIH+ SS++GPHCGLIPIG PS +TTT+GL+WDL++TEM+FGGLISTSN+VKE
Sbjct: 185 LPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKE 244

Query: 241 EKVTVQSDSDLLWTISLK 259
           E +TV+SDSDLLWTIS+K
Sbjct: 245 EIITVESDSDLLWTISIK 256

BLAST of CcUC09G168660 vs. TAIR 10
Match: AT2G44750.1 (thiamin pyrophosphokinase 2 )

HSP 1 Score: 367.9 bits (943), Expect = 7.0e-102
Identity = 176/258 (68.22%), Postives = 207/258 (80.23%), Query Frame = 0

Query: 1   MGVLFHSSEFLLPSSSSSPPDHRRSLSYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60
           M V+ HSS FLLP       D      YALV+LNQ LP+FTPLLW+HA+LRLCADGGANR
Sbjct: 5   MDVMIHSSSFLLPC------DETCGTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANR 64

Query: 61  VFDELPLMLPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL  PH D   +RN YKPDVI+GDMDSIR +VL+FY   GTK+ DES DQDTTDL
Sbjct: 65  IYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDL 124

Query: 121 HKCVAYILQSMPNQEESDLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180
            KC++YI  S  NQE S   IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 125 DKCISYIRHSTLNQESSR--ILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 184

Query: 181 LPRTHHHEIHVRSSVEGPHCGLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKE 240
           LP+TH HEIH+ SS++GPHCGLIPIG PS +TTT+GL+WDL++TEM+FGGLISTSN+VKE
Sbjct: 185 LPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKE 244

Query: 241 EKVTVQSDSDLLWTISLK 259
           E +TV+SDSDLLWTIS+K
Sbjct: 245 EIITVESDSDLLWTISIK 254

BLAST of CcUC09G168660 vs. TAIR 10
Match: AT1G02880.1 (thiamin pyrophosphokinase1 )

HSP 1 Score: 281.2 bits (718), Expect = 8.6e-76
Identity = 132/178 (74.16%), Postives = 152/178 (85.39%), Query Frame = 0

Query: 81  YKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDLHKCVAYILQSMPNQEESDLC 140
           YKPDVI+GDMDSIR +VL+FY   GTK+ DES DQDTTDL KC+ YI  S  NQE S L 
Sbjct: 9   YKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQ 68

Query: 141 ILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHLLPRTHHHEIHVRSSVEGPHC 200
           IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI LLP+TH HEIH++SS+EGPHC
Sbjct: 69  ILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHC 128

Query: 201 GLIPIGMPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKEEKVTVQSDSDLLWTISLK 259
           GLIPIG PS  TTT+GLQWDL++TEM+FGGLISTSN+VKEEK+TV+SDSDLLWTIS+K
Sbjct: 129 GLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIK 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008451592.12.2e-13794.23PREDICTED: thiamine pyrophosphokinase 1 isoform X1 [Cucumis melo][more]
XP_038899470.13.4e-13594.21thiamine pyrophosphokinase 1 [Benincasa hispida] >XP_038899471.1 thiamine pyroph... [more]
XP_023515266.11.9e-13390.70thiamine pyrophosphokinase 1 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_011659355.14.2e-13392.40thiamine pyrophosphokinase 1 isoform X1 [Cucumis sativus] >KGN44926.1 hypothetic... [more]
KAG7030224.15.5e-13390.31Thiamine pyrophosphokinase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
B9DGU72.5e-10470.16Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1[more]
F4IV162.3e-10268.60Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1[more]
Q5JK247.3e-9666.93Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE... [more]
Q60DX11.3e-9264.84Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE... [more]
Q0JMW02.4e-9161.78Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE... [more]
Match NameE-valueIdentityDescription
A0A1S3BSZ41.0e-13794.23Thiamine pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103492826 PE=3 SV=1[more]
A0A0A0KAV42.0e-13392.40Thiamine pyrophosphokinase OS=Cucumis sativus OX=3659 GN=Csa_7G396410 PE=3 SV=1[more]
A0A6J1KV432.7e-13390.31Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111497866 PE=3 SV=1[more]
A0A6J1GPJ26.5e-13289.23Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=3 SV... [more]
A0A6J1JRW15.5e-13188.97Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111488361 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02880.21.8e-10570.16thiamin pyrophosphokinase1 [more]
AT1G02880.31.8e-10570.16thiamin pyrophosphokinase1 [more]
AT2G44750.21.7e-10368.60thiamin pyrophosphokinase 2 [more]
AT2G44750.17.0e-10268.22thiamin pyrophosphokinase 2 [more]
AT1G02880.18.6e-7674.16thiamin pyrophosphokinase1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007373Thiamin pyrophosphokinase, thiamin-binding domainSMARTSM00983TPK_B1_binding_a_2_acoord: 185..251
e-value: 5.0E-12
score: 56.0
IPR007373Thiamin pyrophosphokinase, thiamin-binding domainPFAMPF04265TPK_B1_bindingcoord: 186..252
e-value: 3.1E-23
score: 81.3
IPR007371Thiamin pyrophosphokinase, catalytic domainPFAMPF04263TPK_catalyticcoord: 43..169
e-value: 9.6E-41
score: 138.6
IPR007371Thiamin pyrophosphokinase, catalytic domainCDDcd07995TPKcoord: 29..255
e-value: 2.06031E-59
score: 185.441
IPR016966Thiamin pyrophosphokinase, eukaryoticPIRSFPIRSF031057TPK1coord: 3..261
e-value: 8.7E-69
score: 229.6
IPR036759Thiamin pyrophosphokinase, catalytic domain superfamilyGENE3D3.40.50.10240Thiamin pyrophosphokinase, catalytic domaincoord: 27..168
e-value: 1.8E-45
score: 156.8
IPR036759Thiamin pyrophosphokinase, catalytic domain superfamilySUPERFAMILY63999Thiamin pyrophosphokinase, catalytic domaincoord: 25..177
IPR006282Thiamin pyrophosphokinaseTIGRFAMTIGR01378TIGR01378coord: 30..255
e-value: 3.0E-61
score: 204.4
NoneNo IPR availableGENE3D2.60.120.320coord: 169..259
e-value: 1.0E-30
score: 107.3
NoneNo IPR availablePANTHERPTHR13622THIAMIN PYROPHOSPHOKINASEcoord: 4..258
NoneNo IPR availablePANTHERPTHR13622:SF12THIAMINE PYROPHOSPHOKINASE 1coord: 4..258
IPR036371Thiamin pyrophosphokinase, thiamin-binding domain superfamilySUPERFAMILY63862Thiamin pyrophosphokinase, substrate-binding domaincoord: 179..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC09G168660.1CcUC09G168660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006298 mismatch repair
biological_process GO:0000398 mRNA splicing, via spliceosome
biological_process GO:0000956 nuclear-transcribed mRNA catabolic process
biological_process GO:0016310 phosphorylation
biological_process GO:0009229 thiamine diphosphate biosynthetic process
biological_process GO:0006772 thiamine metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0032300 mismatch repair complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0030983 mismatched DNA binding
molecular_function GO:0030975 thiamine binding
molecular_function GO:0004788 thiamine diphosphokinase activity