CcUC08G159330 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC08G159330
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionAMP-binding domain-containing protein
LocationCicolChr08: 27439367 .. 27445626 (-)
RNA-Seq ExpressionCcUC08G159330
SyntenyCcUC08G159330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTACGAGAATTTTGATCCCTTATTTCCGGACCAGCCGGTGGTCGACCGGTATCTCCCGGTTTGGGCCAGCCTTCCGGCGTTCCGGTCCAAGCCCGCGTTCATATGGTCGGAAGACGGCACTGCCGACGCCCTGAATGAAGGCTCATTTCTGACATACCGGCAACTTCACGACTCCGTTCAGTTAATCTCCGACCAGTTGCTCCGGCAGCTGCGACGCCGCGACACCGTAATTGTATTGTGCTCGGCGGGGCTGGAGCTTGTACAGCTGATTTATGGATGTCAAAGAGCTGGCCTAGTGAGTGTCCCAATCAGCCCGCCCGACCCTTTTCTCGAGAATGAAAATTGTCAGCATCTTGCTAGAGCTCTCTCGCAGACTAAGCCCCGAGCCGCCATAGCTCACCAATCTTACATCAATACGGTTTTTGGGTACGTCGTGAACTATCTTTCAACTTTGAGGTCAATAGTATAAATTTCATCCATTGAAGTACATTTGTTTAGAAAATTAAGGGTTTCTTCGAATTTTATTAGAGAAAAAAATATTTTTAAAGAAAGTATCTCTGTTTAAACGGTAACATTTTCCAAAACAACTTATTTTTAAAATTAAACAATTGAATAGTTAAACCAAATACAGAAGTCTATATATTTAGGTAGATTTATAGTGAAAATTTAGAGGGTATTTGAGATATTGGGTTGGTTATTATAGTTTGTGGTTATTATAGTCATTAGGTTATAATAGTGTATATTTTGGAGGCAAACTATTCTGGTCTAAGTTGTAATAGTATATGTTTGGATAGATTATTTTAGGGATAGGAAATAGTTAAAATCGCAACAAATAGAAAGATATAAAATGAGTAGATCATAGTAAATACTATAGCAAAAAAAAGTTTTGAAATAGTATTTATTGTATTTAATTATAGACCATAGATAGGTATAAATACCACTATTATATTTGGAGCTCCAAATACAAAACTACTTATAACAATCCGCCAACTTCAAATTGGTGTCTTAAACTTAAATATTTCCTCCAAGATTTCTTTTAGACCCTTAAAGATATGTGAGAGTGTCAATAATATAAATATATATATTCTTTTAACATGAATAAGACACATTCATGTTGTCTCCAAGAAGTGCCCTCAGACACCTACCCTATTGATTTCATAATTTACTGGAAAAAACTAAGATATTTAGCCATGGTTAGTTTTTTTTTTTTCATTGATTTTCATTTTTCTTATCTAAACACTTGAACTCTATTCGGATCAACTTGGTGAAAAAAAAAAAAAAAACTATTAATATTAATTCAGAAGAATGAGTAACACATCATTTCTCACTCAATTTTATGTTTCATGTATTTTTTTTCTTTTTTCAAAATAATGGATAACATCTTAGTTAGCTCATTTAAGACCTAAGAATTTTTGTCTAAAATATGGTTAAACTTAAAAGTTTAAATTAACCAATTACAATATTTATATTATCTAATTTACCATGGTTCTATATCAATATTTAAGTGTGTTTGGACAATATCTTTCTTTGTATAATTCATTCAAGTATTCAATTACAAATTTACTACATGAAATATATTTGTTTATTAAAGAAATAATAATTTTTCATTTTTTACAATAGAATAGAATACATGAAAATGAGTATAGCTCACCCTGAAATATTATTTCAAAATATATACTCCGTCAAATACTAAGAAAAACAATTATTAAAAAAAGAAGTTGATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTNCCTCGACCGACAAGAAGCTAGCTTTGTTGTTGCAGAACGTGCAATGGATTTCAATGGACAGTTTAAAACAACCCCACAAGGAAGCAGAGATCAATCAAAACAAATACCAACCCATTCTTTATCACTCTTCCTCTTACTACGGCTGTAAACCAGAGGAAACTTATCTAATCCAATACACCTCAGGCGCCACCGCAATTCCCAAACCGGTGGTTATTACCGCCGGCGCCGCCGCCCACAACGTCCGTGCAGCGAGAAAAGCCTACGACTTAAACCCAAACGACGTCATCGTTTCTTGGCTCCCACAATACCACGACTGTGGCCTCATGTTCCTTCTCTTAACCGTCATCACTGGTGCCACGTGCGTGCTAACCTCACCCATCTCTTTCGTCACCCGCCCAACCACGTGGCTCCACCTCATCACCGCTTTCAAAGCCACGTGCACTCCCGTGCCGTCTTTCACTCTCCCACTCGTACTCAAGCGGGTGAAGGAAGAAAGTCCTTTGATTAGGGGTTTGGATTTGCGGAGCATGAGGAATTTGATTTTGATTAATGAACCGATTTACCGGTCGGCAGTCGAGGAGTTTGTGGAGGTGTTTAAGGCGGTTGGGTTGGACCCGGGTTGCGTTTCTCCGTCATATGGGTTGGCGGAGAATTGCACTTTTGTTTCGACGGCGTGGTGCGGCGGTGACCGGAGGTGGTGGTTCCCGACGATGCCGAGCTACCGGAAGTTGCTGCCTTCTGCGAGGTTGAAGGATGGATGGTGTAGGGAAATTGAGGTGGTGGTTGTTAATGGCGAAACTGGGGAGGTGGTTGAGGATGGCGTTGAAGGAGAGATTTGGGTCTCGTCGCCCAGTAATGCCTCAGGTTATTTTCTTCTCTACTTTACCACTCTCAGTATTTATCTATAGTTTGATTAAAATTTAGCTAGATTAAATTTTAATTATAAACCATTTATATCTTATTTAATTAGCACGAGTGATTTTTTTTTTTTTTTTTTTTTTTTTAAAGGTAAAACTCATATAAGTTAGAAATATCAAAATTACCTTCTATATTTATCAATCTTTTTGGAAAAATTAAGAGACATCAAGTGCATCATGAGTTACACCCATCTTTTTGCAGTTTACTCCAACAACATACTAAAAGAAAAATTAAAGAAAAAATTTATCGCATAATAACCTGTGATTCGATGTTTGAGTAGACTAATAACATGTTTGAGATTAATTTTAAAATGGTTAGATCGATTTTTTTCTTTTCAAAATCACTTGTAAACACTTTTAATCATTTAAAATCAATTTTAATAGCATAATAGTTGTATTTAAAAGTGTAAATTAAAGTTTTAGACTCCGTTTGATAACCATTTTATCTTTTGTTTTTTGTTTTTGAAAATTAGCCCACTTACTCTCAAATTCTCACAATGGTTTGCATTTTTCTCAAGTAAGAGAGTTGAGTTTTTAGTTAAATTTCAACAAAAAAAAAAAAAAAAGGTTTTTAAAGTTTTTTTTAGTTTTAAAAACCTAGCTTGGTTTTTTAAAACAATGGTAAAAAGTAGATGATAAAGTAATAAATTTAGAGGTGGAAATGGTATTTTATAGGCTTACCTTTCAAAAACCAAAAACCAAAAACTAGATCTCATTTGGTAACCATTTGATATTTTATTTTTGTTTTTGAAAATCAAGTATATAGACACTACTTCCACCTTCAAATTTCTTTATCTATTATCGACTTTTTACCAATGATTTTTAAAAACCATGCCAAATTTTGAAAACTAAAAAAAGTAACTTTAAAAACTTATATTAGTTTTTGAAATTTGGCTAAGAATATTCAACCATTATACTTAGGAAAGATACGAACCATCGTATAAAGTAATAATTTTCAAAAAAAAAAAAAAATAGTCATCAAATGAGTCTTCGATGGTTACCAAACAAACCTTTATTGTTTTCAATGATAAAAAGTATATCACTCCTAAACATGTACTAAAAATGGAAACTAAAAAATCCAACCAAATATTTTTGAAGTTTTAAACATACAGATTATTGAGCTTCAACTTGCCTTCTAATTATGGTTTTAGGGTTTCTATTCTATGTTTTTAAATTCACTGAGTTTAGCGAATATGTGTTTGGCAAACCAACCTGAATTTTGTTTATGGATTATATGATTCAAATCGTGTAAATTTTTGAAAAACATTTTTTATGTTTTCATTATGCCCAAAATTTGAATTTTAAAATTTTAAATGCATAATTATATTAAATAAAGATAAAAACATCTAGAAATATAGTATGTCATGTTATAAACTCAATTCAATTTTTTTAATTAAAATGAGTAATATGTAATTATTATAAATTAGGTTAAATTCCAAATTTGGTTCCTTTAGTTTGGATAAAGTTACAAGTTAGTTCATATGGTTTTAAAATTTAGAATTTAGTTCTCATGATTTGATACAACTTTATATATTGTCCATATACTATAGTTTGTTAAATCCTCTTAAATAATCTATGTCATAGCTTATGAAGCCTTTACCAAAGTATAGGAGTTAAATTCTAATTATAAACCCTAGGACCTAAATTTTAACTTCTTCCAAACCATAGGAACAAAAATTTCAATTTACCCCATAAATTATATAATATTACAAAATAATAATTATACAATTCTTTTTAACATAAAACCACGTCCATAAATAAATTAATAATAGTTTATTGTTAACAAATATTTAGCGGGTTACAAATAATTTGCAATTGTTTAAATTAGAATCTAATTTTTTAATTTTGATACCAAACACATATCAGAAAACATAAAATACAACCTTATTTTTATTTAATCTCTTATTTTCAAATTTTTGTTTTCAGATTTTCTACCAAACAAACCCTTAATAACTCAAATAATCGGTGGAATTCCTTTACTTATTAAATGTAATAAAAAAAATATATATTAATAAAGTCACTTCTTATACAAAGTCAATATAACTAATAGATGTGTAGGCAAGGTTCCTTTTTATTCTTTAAACTAGAGGCAGGGGAGGAAAACTACTAAAATTTTCGGCCGGAGGGTTTTATTTATTTTATCTTTTATTTTTCTGCAAAATAATAACGTGGATTTTTTTTTTCTTTTTTTAAATTTTTGGTTGTTTGTTTGTTTTATATATGATTATCTATTCAAATCTATTTTTCAAAGACGTGGTCAAATATTTTAGAGAAATGTAGTTTTAAAAAAATGTTAAAAAAGAATGGCGGTAGAAATAAGAATATAGAAATGGTGTGATACATAGTCGTCACGTTTTATATATTAAAAAAATACAATTAAAGAAATGATATTTAAATGGGCTAAATTATGCTAAATACAAATAATAATGATAATTATAATAATTCTTGTCCATATACTAGTCAACAAGAATTTTTATTAATTTTCCGTGATGAGTAAATGCTAGTCAACAAGAGTTTTTTCATTAATACAACTATTGTTAATAGAATAAAAAAAATCTTTTAAAAACTATTTTTTTAAATATGGGTAGAAAGTATATAATAAAATAAGATATTTGAATTTTAATTTTTTTTAAAATTGAGCTTGTTTTCTCATAATTTTAATATTTTCTTATTTAAATATTTGAATTTTTAAGCTAATTTTAACAATTTTTTCTTGAAATTTTGGCATTGGTTTCAAAGAATATGCTAAAATGTAACCAACCAAATATAGAAATTAAGAGATAGAGTAGGAGTGACGTGAATAACTTAAATTTTCAAAAATTAAAAATCAACATACCTCCGTACTCTCTTTTCTTAAAATAATAAAAATAATAATTTAAAAAAGAAAAAATAATCTTTACAAAACAAGTTTTACTTCTGTTTGTGAATTGGCAGTCTGAAAGGAAAGCTCTCACCAAAAAGGATATTTTATCCTCCCTCAGGTTACCTTGATCATCCATCTGTAACCAGAGAGACATTTCACAGTAAAATCAACAACAAATCAAGCCCTAATTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGCGCCGACAGGTTCCTGTTCGTGATCGGACGGTGTTCAGACGTCATCGAATTCAACAACCATGAAATCCACCCTCATTACATCGAATCCATAGCTTACAACAATTCCTCTGCCTATCTTCGAGGCGGCTGCATCGCCGCCGTCAAGATATCGGACACAATCGCCCTCGTTGCCGAGATGCAGAGAGACGACAAAAACGATGCCGGATTGCTGAGGAAAATTTGCGAAGAGATTAGAAAAGCGTTGTTGATTGAAGAGGGAATTGAATTAGGGGTTGTGGTTTTGGTGAAAAGGGGAAACGTCCCCAAAACGACGTCGGGGAAAGTGAAGAGATGGGCGGTGAAGGAGAAGCTCGACGGCGGAGGATTCGGCGTTTTGATGGCCGTGAAATTTGATAAAAACTGTGACGTTTTAAAGGATTCAGAGAGAAAAGAAGAGTTTCGAACTCGTCCTCTTCTGCCATCTCTTCTTTAATTTGAGTGTTTATTTTAAACTTCTG

mRNA sequence

ATGTTGTACGAGAATTTTGATCCCTTATTTCCGGACCAGCCGGTGGTCGACCGGTATCTCCCGGTTTGGGCCAGCCTTCCGGCGTTCCGGTCCAAGCCCGCGTTCATATGGTCGGAAGACGGCACTGCCGACGCCCTGAATGAAGGCTCATTTCTGACATACCGGCAACTTCACGACTCCGTTCAGTTAATCTCCGACCAGTTGCTCCGGCAGCTGCGACGCCGCGACACCGTAATTGTATTGTGCTCGGCGGGGCTGGAGCTTGTACAGCTGATTTATGGATGTCAAAGAGCTGGCCTAGTGAGTGTCCCAATCAGCCCGCCCGACCCTTTTCTCGAGAATGAAAATTGTCAGCATCTTGCTAGAGCTCTCTCGCAGACTAAGCCCCGAGCCGCCATAGCTCACCAATCTTACATCAATACGGTTTTTGGTNCCTCGACCGACAAGAAGCTAGCTTTGTTGTTGCAGAACGTGCAATGGATTTCAATGGACAGTTTAAAACAACCCCACAAGGAAGCAGAGATCAATCAAAACAAATACCAACCCATTCTTTATCACTCTTCCTCTTACTACGGCTGTAAACCAGAGGAAACTTATCTAATCCAATACACCTCAGGCGCCACCGCAATTCCCAAACCGGTGGTTATTACCGCCGGCGCCGCCGCCCACAACGTCCGTGCAGCGAGAAAAGCCTACGACTTAAACCCAAACGACGTCATCGTTTCTTGGCTCCCACAATACCACGACTGTGGCCTCATGTTCCTTCTCTTAACCGTCATCACTGGTGCCACGTGCGTGCTAACCTCACCCATCTCTTTCGTCACCCGCCCAACCACGTGGCTCCACCTCATCACCGCTTTCAAAGCCACGTGCACTCCCGTGCCGTCTTTCACTCTCCCACTCGTACTCAAGCGGGTGAAGGAAGAAAGTCCTTTGATTAGGGGTTTGGATTTGCGGAGCATGAGGAATTTGATTTTGATTAATGAACCGATTTACCGGTCGGCAGTCGAGGAGTTTGTGGAGGTGTTTAAGGCGGTTGGGTTGGACCCGGGTTGCGTTTCTCCGTCATATGGGTTGGCGGAGAATTGCACTTTTGTTTCGACGGCGTGGTGCGGCGGTGACCGGAGGTGGTGGTTCCCGACGATGCCGAGCTACCGGAAGTTGCTGCCTTCTGCGAGGTTGAAGGATGGATGGTGTAGGGAAATTGAGGTGGTGGTTGTTAATGGCGAAACTGGGGAGGTGGTTGAGGATGGCGTTGAAGGAGAGATTTGGGTCTCGTCGCCCAGTAATGCCTCAGGTTACCTTGATCATCCATCTGTAACCAGAGAGACATTTCACAGTAAAATCAACAACAAATCAAGCCCTAATTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGCGCCGACAGGTTCCTGTTCGTGATCGGACGGTGTTCAGACGTCATCGAATTCAACAACCATGAAATCCACCCTCATTACATCGAATCCATAGCTTACAACAATTCCTCTGCCTATCTTCGAGGCGGCTGCATCGCCGCCGTCAAGATATCGGACACAATCGCCCTCGTTGCCGAGATGCAGAGAGACGACAAAAACGATGCCGGATTGCTGAGGAAAATTTGCGAAGAGATTAGAAAAGCGTTGTTGATTGAAGAGGGAATTGAATTAGGGGTTGTGGTTTTGGTGAAAAGGGGAAACGTCCCCAAAACGACGTCGGGGAAAGTGAAGAGATGGGCGGTGAAGGAGAAGCTCGACGGCGGAGGATTCGGCGTTTTGATGGCCGTGAAATTTGATAAAAACTGTGACGTTTTAAAGGATTCAGAGAGAAAAGAAGAGTTTCGAACTCGTCCTCTTCTGCCATCTCTTCTTTAATTTGAGTGTTTATTTTAAACTTCTG

Coding sequence (CDS)

ATGTTGTACGAGAATTTTGATCCCTTATTTCCGGACCAGCCGGTGGTCGACCGGTATCTCCCGGTTTGGGCCAGCCTTCCGGCGTTCCGGTCCAAGCCCGCGTTCATATGGTCGGAAGACGGCACTGCCGACGCCCTGAATGAAGGCTCATTTCTGACATACCGGCAACTTCACGACTCCGTTCAGTTAATCTCCGACCAGTTGCTCCGGCAGCTGCGACGCCGCGACACCGTAATTGTATTGTGCTCGGCGGGGCTGGAGCTTGTACAGCTGATTTATGGATGTCAAAGAGCTGGCCTAGTGAGTGTCCCAATCAGCCCGCCCGACCCTTTTCTCGAGAATGAAAATTGTCAGCATCTTGCTAGAGCTCTCTCGCAGACTAAGCCCCGAGCCGCCATAGCTCACCAATCTTACATCAATACGGTTTTTGGTNCCTCGACCGACAAGAAGCTAGCTTTGTTGTTGCAGAACGTGCAATGGATTTCAATGGACAGTTTAAAACAACCCCACAAGGAAGCAGAGATCAATCAAAACAAATACCAACCCATTCTTTATCACTCTTCCTCTTACTACGGCTGTAAACCAGAGGAAACTTATCTAATCCAATACACCTCAGGCGCCACCGCAATTCCCAAACCGGTGGTTATTACCGCCGGCGCCGCCGCCCACAACGTCCGTGCAGCGAGAAAAGCCTACGACTTAAACCCAAACGACGTCATCGTTTCTTGGCTCCCACAATACCACGACTGTGGCCTCATGTTCCTTCTCTTAACCGTCATCACTGGTGCCACGTGCGTGCTAACCTCACCCATCTCTTTCGTCACCCGCCCAACCACGTGGCTCCACCTCATCACCGCTTTCAAAGCCACGTGCACTCCCGTGCCGTCTTTCACTCTCCCACTCGTACTCAAGCGGGTGAAGGAAGAAAGTCCTTTGATTAGGGGTTTGGATTTGCGGAGCATGAGGAATTTGATTTTGATTAATGAACCGATTTACCGGTCGGCAGTCGAGGAGTTTGTGGAGGTGTTTAAGGCGGTTGGGTTGGACCCGGGTTGCGTTTCTCCGTCATATGGGTTGGCGGAGAATTGCACTTTTGTTTCGACGGCGTGGTGCGGCGGTGACCGGAGGTGGTGGTTCCCGACGATGCCGAGCTACCGGAAGTTGCTGCCTTCTGCGAGGTTGAAGGATGGATGGTGTAGGGAAATTGAGGTGGTGGTTGTTAATGGCGAAACTGGGGAGGTGGTTGAGGATGGCGTTGAAGGAGAGATTTGGGTCTCGTCGCCCAGTAATGCCTCAGGTTACCTTGATCATCCATCTGTAACCAGAGAGACATTTCACAGTAAAATCAACAACAAATCAAGCCCTAATTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGCGCCGACAGGTTCCTGTTCGTGATCGGACGGTGTTCAGACGTCATCGAATTCAACAACCATGAAATCCACCCTCATTACATCGAATCCATAGCTTACAACAATTCCTCTGCCTATCTTCGAGGCGGCTGCATCGCCGCCGTCAAGATATCGGACACAATCGCCCTCGTTGCCGAGATGCAGAGAGACGACAAAAACGATGCCGGATTGCTGAGGAAAATTTGCGAAGAGATTAGAAAAGCGTTGTTGATTGAAGAGGGAATTGAATTAGGGGTTGTGGTTTTGGTGAAAAGGGGAAACGTCCCCAAAACGACGTCGGGGAAAGTGAAGAGATGGGCGGTGAAGGAGAAGCTCGACGGCGGAGGATTCGGCGTTTTGATGGCCGTGAAATTTGATAAAAACTGTGACGTTTTAAAGGATTCAGAGAGAAAAGAAGAGTTTCGAACTCGTCCTCTTCTGCCATCTCTTCTTTAA

Protein sequence

MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDSVQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHLARALSQTKPRAAIAHQSYINTVFGXSTDKKLALLLQNVQWISMDSLKQPHKEAEINQNKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKGADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEMQRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDGGGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSLL
Homology
BLAST of CcUC08G159330 vs. NCBI nr
Match: XP_038884878.1 (long-chain-fatty-acid--AMP ligase FadD26-like [Benincasa hispida])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 544/630 (86.35%), Postives = 585/630 (92.86%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTA +LN+GSFLTY+QLHDS
Sbjct: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQ ISDQLLRQLRRRDTV VLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENC HL
Sbjct: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120

Query: 121 ARALSQTKPRAAIAHQSYINTVF----GXSTDKKLALLLQNVQWISMDSLKQPHKEAEIN 180
           ARALSQTKPRAAIAHQSYI T+F       TD+KLALLLQ+VQWISMDSLKQP K+AE+N
Sbjct: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180

Query: 181 QNKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNP 240
           Q+K+QPILYHSSSYYGC P+E YLIQYTSGATAIPKPVV+TAGAAAHNVR ARKAY+LNP
Sbjct: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240

Query: 241 NDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPS 300
           NDVIVSWLPQYHDCGLMFLLL+VITGATCVLTSP+SFVT P  WLHLITAFKATCTPVPS
Sbjct: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300

Query: 301 FTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPS 360
           FTLPLVLKRVK+E+P  +GLDLRS+RNLILINEPIYR+AVEEFV+ FKAVGLDPGCVSPS
Sbjct: 301 FTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPS 360

Query: 361 YGLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVNGETGEVVE 420
           YGLAENCTFVSTAW GG RRWWFP MP+YRKLLPS RL+DG C EI+VVVVNGETGEVVE
Sbjct: 361 YGLAENCTFVSTAWSGG-RRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVVE 420

Query: 421 DGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKGADRFLFVI 480
           DGVEGEIWVSSPSNASGYL HPS+T++TFHSKINNKSSPNF+RTGDRGVI+GADRFLFVI
Sbjct: 421 DGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVI 480

Query: 481 GRCSDVIEFNN--HEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEMQRDDKND 540
           GRCSD+I+FNN   EIHPHYIES AYNNSSAYLRGGCIAAVKIS+TIA+VAEMQRDDKND
Sbjct: 481 GRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEMQRDDKND 540

Query: 541 AGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDGGGFGVLM 600
           A LLRKICEEIRKA+ IEEG ELG+VVLVKRGN+PKTTSGKVKRWAVKEKL GGG GVLM
Sbjct: 541 AELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLGVLM 600

Query: 601 AVKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
           A+ F+KNC+VL   +R+EE RTRP L SLL
Sbjct: 601 AIGFEKNCEVL---QREEELRTRPALLSLL 626

BLAST of CcUC08G159330 vs. NCBI nr
Match: XP_011649625.1 (uncharacterized protein LOC105434645 [Cucumis sativus] >KGN62605.1 hypothetical protein Csa_021877 [Cucumis sativus])

HSP 1 Score: 1036.6 bits (2679), Expect = 8.9e-299
Identity = 524/629 (83.31%), Postives = 562/629 (89.35%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           MLYENFDPLFP+QPVVDRYLPVWASLPAF+SKPAFIWSEDGTA A+NE SFLTYRQLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQLI++QLLRQLRRRDTV+VLCSAGL+ VQLIYGCQRAGLVSVPISPPD F ENENC HL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 ARALSQTKPRAAIAHQSYINTVF---GXSTDKKLALLLQNVQWISMDSLKQPHKEAEINQ 180
           ARALSQTKPRAAIAHQSYINTVF     S DKKLALLL+ V+WISM+SLKQPHKE+E+NQ
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSSADKKLALLLKGVRWISMESLKQPHKESELNQ 180

Query: 181 NKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPN 240
           +K+Q   YHSSSY GC P++ YLIQYTSGATAI K VVITAGAAAHNVRAARKAYDLNPN
Sbjct: 181 HKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNPN 240

Query: 241 DVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSF 300
           D+IVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVT+P TWLHLITAFKATCTPVPSF
Sbjct: 241 DLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSF 300

Query: 301 TLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSY 360
           TLPLVLKRV   S     LDLRS+RNLILINEPIYRS VEEFVEVFK VGLDPGCVSPSY
Sbjct: 301 TLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSPSY 360

Query: 361 GLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVNGETGEVVED 420
           GLAENCTFVSTAWCGG     FP MPSYR+LLPS RL+DG C+EIEVVVVN ETGEVVED
Sbjct: 361 GLAENCTFVSTAWCGGG---GFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVED 420

Query: 421 GVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKGADRFLFVIG 480
           GVEGEIWVSSPSNA GYL HPSVT ETFHSK+ NKSS NFVRTGDRGVIKG+DRFLFVIG
Sbjct: 421 GVEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGSDRFLFVIG 480

Query: 481 RCSDVIEFNNH--EIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEMQRDDKNDA 540
           RCSDVI+ NN+  +IHPHYIES +YNN SAYLRGGCIAAVK+S TIALVAEMQRDD++DA
Sbjct: 481 RCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEMQRDDRHDA 540

Query: 541 GLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDGGGFGVLMA 600
            LLRKICE+IRKA+LIEEGIELG+VVLVKRGNV KTTSGKVKRW VKEKL GGG GVLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 VKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
            +F KNC    D +RK EF TRP+L S+L
Sbjct: 601 FEFGKNC---VDLKRKGEFETRPVLISML 623

BLAST of CcUC08G159330 vs. NCBI nr
Match: XP_022962563.1 (uncharacterized protein LOC111462961 [Cucurbita moschata])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.4e-291
Identity = 515/637 (80.85%), Postives = 552/637 (86.66%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD LNEGSFLTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQ ISD+L+RQ+RRRDTV++LCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC HL
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHHL 133

Query: 121 ARALSQTKPRAAIAHQSYINTVFG----XSTDKKLALLLQNVQWISMDSLK-----QPHK 180
           ARALSQTKPRAAIAHQ+YI +VF       TD KLALLLQ+VQWISM++L+     +  +
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEADQ 193

Query: 181 EAEINQNKYQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARK 240
              +  +K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVRAARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 253

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDP 360
           CTPVPSFTLPLVLKRVKEE+P   GLDL S+RNLILINEP+YRS VEEFV+VFKAVGL+P
Sbjct: 314 CTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLNP 373

Query: 361 GCVSPSYGLAENCTFVSTAWCGGD--RRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVN 420
           GCVSPSYGLAENCTFVSTAW GG+  RR WFP MPSYRKLLPSARL D    EIEVVVVN
Sbjct: 374 GCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVVN 433

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKG 480
           GETGE+VEDGVEGEIW+SSPSNASGYL HPS+TR+TFH K++NKSS  FVRTGDRGVIKG
Sbjct: 434 GETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 493

Query: 481 ADRFLFVIGRCSDVIEF-NNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEM 540
            DRFLFVIGRCSDVI   NN EIHPHYIESIAYNN S+YLRGGC+AA+KISDTIA+VAEM
Sbjct: 494 TDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVAEM 553

Query: 541 QRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDG 600
           QR DKNDA  LRKICE IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVKRWA KEKL G
Sbjct: 554 QRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKLAG 613

Query: 601 GGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
           GG  VLMAVKF K C V KD E       RPLL SLL
Sbjct: 614 GGMSVLMAVKFGKTCGVTKDLE-----TNRPLLLSLL 645

BLAST of CcUC08G159330 vs. NCBI nr
Match: XP_023546350.1 (uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1003.4 bits (2593), Expect = 8.4e-289
Identity = 511/637 (80.22%), Postives = 550/637 (86.34%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD LNEGSFLTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQ IS+QL+RQ+RRRDTVIVLCS GL+LVQLIYGCQRAGLVSVPISPPDP  EN+NC HL
Sbjct: 74  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENKNCHHL 133

Query: 121 ARALSQTKPRAAIAHQSYINTVFG----XSTDKKLALLLQNVQWISMDSLK-----QPHK 180
           ARALSQTKPRAAIAHQ+YI +VF       TD KLALLL+ VQWISM++L+     +  +
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLRGVQWISMETLRTCTAAEADQ 193

Query: 181 EAEINQNKYQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARK 240
              +  +K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVR ARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVARK 253

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDP 360
           CTPVPSFTLPLVLKRVKEE+P   GLDL S+RNLILINEP+YRS VEEFV+VFK VGL+P
Sbjct: 314 CTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKTVGLNP 373

Query: 361 GCVSPSYGLAENCTFVSTAWCGGD--RRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVN 420
           GCVSPSYGLAENCTFVSTAW GG+  RR WFP MPSYRKLLP A+L D    EIEVVVVN
Sbjct: 374 GCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPCAKLTDESSAEIEVVVVN 433

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKG 480
           GETGE+V+DGVEGEIW+SSPSNASGYL HPS+TR+TFH K++NKSS  FVRTGDRGVIKG
Sbjct: 434 GETGEIVDDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 493

Query: 481 ADRFLFVIGRCSDVIEFNNH-EIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEM 540
            DRFLFVIGRCSDVI+ NN+ EIHPHYIESIAYNN SAYLRGGC+AA+KISDTIA+VAEM
Sbjct: 494 TDRFLFVIGRCSDVIKLNNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKISDTIAVVAEM 553

Query: 541 QRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDG 600
           QRDDKNDA  LRKICE IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVKRWA KEKL G
Sbjct: 554 QRDDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKLAG 613

Query: 601 GGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
           GG  VLMAVKF   C V KD E       RPLL SLL
Sbjct: 614 GGMSVLMAVKFGTTCGVTKDLE-----GNRPLLLSLL 645

BLAST of CcUC08G159330 vs. NCBI nr
Match: KAG7029389.1 (fadD26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.4e-288
Identity = 510/637 (80.06%), Postives = 548/637 (86.03%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD LNEGSFLTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQ ISD+L+RQ+RRRDTV++LCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC HL
Sbjct: 61  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHHL 120

Query: 121 ARALSQTKPRAAIAHQSYINTVFGXST----DKKLALLLQNVQWISMDSLK-----QPHK 180
           ARALSQTKP AAIAHQ+YI +VF   +    D KLALLL++VQWISM+SL+     +  +
Sbjct: 121 ARALSQTKPWAAIAHQAYIASVFRYLSLSPIDGKLALLLRSVQWISMESLRTCTAAEADQ 180

Query: 181 EAEINQNKYQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARK 240
              +   K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVR ARK
Sbjct: 181 VVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FKAT
Sbjct: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDP 360
           CTPVPSFTLPLVLKRVKEE+P   GLDL S+RNLILINEP+YRS VEEFV+VFKAVGL+P
Sbjct: 301 CTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360

Query: 361 GCVSPSYGLAENCTFVSTAWCGGD--RRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVN 420
           GCVSPSYGLAENCTFVSTAW GG+  RR WFP MPSYRKLLPSARL D    EIEVVVVN
Sbjct: 361 GCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVVN 420

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKG 480
           GETGE+VEDGVEGEIW+SSPSNASGYL HPS+TR+TFH K++NKSS  FVRTGDRGVIKG
Sbjct: 421 GETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 480

Query: 481 ADRFLFVIGRCSDVIEF-NNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEM 540
            DRFLFVIGRCSDVI   NN EIHPHYIESIAYNN S+YLRGGC+AA+KISDTIA+VAEM
Sbjct: 481 TDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVAEM 540

Query: 541 QRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDG 600
           QR DKNDA  LRKICE IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVKRWA KEKL G
Sbjct: 541 QRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKLAG 600

Query: 601 GGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
           GG  VLMAVKF K C + KD E       RPLL S L
Sbjct: 601 GGMSVLMAVKFGKTCGITKDLE-----TNRPLLLSFL 632

BLAST of CcUC08G159330 vs. ExPASy Swiss-Prot
Match: B2HIN2 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 4.6e-48
Identity = 172/614 (28.01%), Postives = 278/614 (45.28%), Query Frame = 0

Query: 14  PVVDRYLPVWASLPAFR--SKPAFIWSEDGTADALNEGSFLTYRQLHDSVQLISDQLLRQ 73
           PV DR +P      A +  ++ AF +  D   D       LT+ Q++    +++D+L   
Sbjct: 2   PVTDRSIPSLLKEQADQRPNETAFTFL-DYDLDPNGFAETLTWSQVYARACVVADELTMY 61

Query: 74  LRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHLARALSQTKPRA 133
               D V +L   GLE +    G  +AG + VP+S P   + +E    ++  L  ++P A
Sbjct: 62  GVPGDRVAILAPQGLEYIVAFLGALQAGFIGVPLSTPQYGVHDE---RVSAVLRDSQPVA 121

Query: 134 AIAHQSYIN--TVFGXSTDKKLALLLQNVQWISMDSLKQPHKEAEINQNKYQPILYHSSS 193
            +   + +   T +  S D + A  +  V  + +D+   P  +  + Q         S S
Sbjct: 122 ILTTSAVVGDVTKYASSQDGQPAPSVIEVDLLDLDT---PRPQQALPQPA-------SGS 181

Query: 194 YYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAY----DLNPND-VIVSWL 253
            Y         +QYTSG+T  P  V+++      NV  +   Y    D  P D  +VSWL
Sbjct: 182 AY---------LQYTSGSTRTPAGVIVSHENVIANVTQSLYGYFGGPDKFPADTTVVSWL 241

Query: 254 PQYHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVL 313
           P +HD GL+  +   ++TG T VL SP+SF+ RP  W+ L+ +     +  P+F   L +
Sbjct: 242 PLFHDMGLILGICAPLVTGCTAVLLSPMSFLRRPARWMQLLASHPKCFSAAPNFAFELAV 301

Query: 314 KRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENC 373
           +R  +E   + GLDL  +  ++  +E I+ + ++ F E F    L P  V PSYGLAE  
Sbjct: 302 RRTTDED--LAGLDLGDVLGIVSGSERIHVATIKRFTERFAPFNLSPAAVRPSYGLAEAT 361

Query: 374 TFVSTAWCGGDRRWWFPTMPSYRKL-------------LPSARLKDGWCREIEVVVVNGE 433
            +V+    G   R        Y  L             + +  +  G      V +VN E
Sbjct: 362 LYVAAPEPGTTPR---TVRFDYESLTAGHARPCRADGSVGTELISYGSPDPSAVRIVNPE 421

Query: 434 TGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKS--SPN--FVRTGDRGVI 493
           T      G  GEIW      A GY   P  +  TF+++I N +  +P   ++RTGD GV+
Sbjct: 422 TMIENPSGTVGEIWAHGEHVAMGYWQKPEQSDRTFNARIVNPAPGTPEGPWLRTGDLGVM 481

Query: 494 KGADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTI----- 553
              +  LF++GR  D++  +    +P  IE+         + GG +AA+ + D I     
Sbjct: 482 SNGE--LFIMGRIKDLVIVDGRNHYPDDIEATIQE-----ITGGRVAAIAVPDNITEQLV 541

Query: 554 ALVAEMQRDDKNDAGL--LRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRW 594
           A++   +R    +  +  LR +  EI  A+     + +  VVLV  G++P TTSGK++R 
Sbjct: 542 AIIELKRRGASAEEAMVKLRSVKREITSAISKSHSLRVADVVLVPPGSIPITTSGKIRRA 580

BLAST of CcUC08G159330 vs. ExPASy Swiss-Prot
Match: Q7TXM1 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.0e-47
Identity = 167/609 (27.42%), Postives = 273/609 (44.83%), Query Frame = 0

Query: 14  PVVDRYLPVWASLPAFRSKPAFIWSE-DGTADALNEGSFLTYRQLHDSVQLISDQLLRQL 73
           PV DR +P      A +   +  ++  D  +D       LT+ Q++    +I+++L    
Sbjct: 2   PVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCG 61

Query: 74  RRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHLARALSQTKPRAA 133
              D V VL   GLE V    G  +AG ++VP+S P   + ++    ++  L  +KP A 
Sbjct: 62  LPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDD---RVSAVLQDSKPVAI 121

Query: 134 IAHQSYIN--TVFGXSTDKKLALLLQNVQWISMDSLKQPHKEAEINQNKYQPILYHSSSY 193
           +   S +   T +  S D + A ++  V  + +DS +Q    +            H+ + 
Sbjct: 122 LTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSR----------QHTGAA 181

Query: 194 YGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPNDV----IVSWLPQ 253
           Y         +QYTSG+T  P  V+++      NV  +   Y  +P  +    +VSWLP 
Sbjct: 182 Y---------LQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPL 241

Query: 254 YHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKR 313
           YHD GL+  +   ++     VL SP+SF+ RP  W+ L+       +  P+F   L ++R
Sbjct: 242 YHDMGLILGICAPLVARRRAVLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRR 301

Query: 314 VKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTF 373
             ++   + GLDLR +  ++  +E I+ + V  F+E F    L P  + PSYGLAE   +
Sbjct: 302 TSDQD--MAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLY 361

Query: 374 VSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCREI-------------EVVVVNGETG 433
           V+    G   +        Y +L        G    +              V +VN ET 
Sbjct: 362 VAAPEAGAAPK---TVRFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETM 421

Query: 434 EVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINN--KSSPN--FVRTGDRGVIKG 493
                GV GEIWV       GY   P  T + F +K+ +   ++P   ++RTGD GVI  
Sbjct: 422 VENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVI-- 481

Query: 494 ADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIA--LVAE 553
           +D  LF++GR  D++  +    +P  IE+         + GG  AA+ + D I   LVA 
Sbjct: 482 SDGELFIMGRIKDLLIVDGRNHYPDDIEATIQE-----ITGGRAAAIAVPDDITEQLVAI 541

Query: 554 MQRDDKNDAG-----LLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAV 591
           ++   +          LR +  E+  A+     + +  +VLV  G++P TTSGK++R A 
Sbjct: 542 IEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGKIRRSAC 576

BLAST of CcUC08G159330 vs. ExPASy Swiss-Prot
Match: P9WQ42 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD26 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 6.7e-47
Identity = 166/609 (27.26%), Postives = 273/609 (44.83%), Query Frame = 0

Query: 14  PVVDRYLPVWASLPAFRSKPAFIWSE-DGTADALNEGSFLTYRQLHDSVQLISDQLLRQL 73
           PV DR +P      A +   +  ++  D  +D       LT+ Q++    +I+++L    
Sbjct: 2   PVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCG 61

Query: 74  RRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHLARALSQTKPRAA 133
              D V VL   GLE V    G  +AG ++VP+S P   + ++    ++  L  +KP A 
Sbjct: 62  LPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDD---RVSAVLQDSKPVAI 121

Query: 134 IAHQSYIN--TVFGXSTDKKLALLLQNVQWISMDSLKQPHKEAEINQNKYQPILYHSSSY 193
           +   S +   T +  S D + A ++  V  + +DS +Q    +            H+ + 
Sbjct: 122 LTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSR----------QHTGAA 181

Query: 194 YGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPNDV----IVSWLPQ 253
           Y         +QYTSG+T  P  V+++      NV  +   Y  +P  +    +VSWLP 
Sbjct: 182 Y---------LQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPL 241

Query: 254 YHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKR 313
           YHD GL+  +   ++     +L SP+SF+ RP  W+ L+       +  P+F   L ++R
Sbjct: 242 YHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRR 301

Query: 314 VKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTF 373
             ++   + GLDLR +  ++  +E I+ + V  F+E F    L P  + PSYGLAE   +
Sbjct: 302 TSDQD--MAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLY 361

Query: 374 VSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCREI-------------EVVVVNGETG 433
           V+    G   +        Y +L        G    +              V +VN ET 
Sbjct: 362 VAAPEAGAAPK---TVRFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETM 421

Query: 434 EVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINN--KSSPN--FVRTGDRGVIKG 493
                GV GEIWV       GY   P  T + F +K+ +   ++P   ++RTGD GVI  
Sbjct: 422 VENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVI-- 481

Query: 494 ADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIA--LVAE 553
           +D  LF++GR  D++  +    +P  IE+         + GG  AA+ + D I   LVA 
Sbjct: 482 SDGELFIMGRIKDLLIVDGRNHYPDDIEATIQE-----ITGGRAAAIAVPDDITEQLVAI 541

Query: 554 MQRDDKNDAG-----LLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAV 591
           ++   +          LR +  E+  A+     + +  +VLV  G++P TTSGK++R A 
Sbjct: 542 IEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGKIRRSAC 576

BLAST of CcUC08G159330 vs. ExPASy Swiss-Prot
Match: P9WQ43 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 6.7e-47
Identity = 166/609 (27.26%), Postives = 273/609 (44.83%), Query Frame = 0

Query: 14  PVVDRYLPVWASLPAFRSKPAFIWSE-DGTADALNEGSFLTYRQLHDSVQLISDQLLRQL 73
           PV DR +P      A +   +  ++  D  +D       LT+ Q++    +I+++L    
Sbjct: 2   PVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCG 61

Query: 74  RRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHLARALSQTKPRAA 133
              D V VL   GLE V    G  +AG ++VP+S P   + ++    ++  L  +KP A 
Sbjct: 62  LPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDD---RVSAVLQDSKPVAI 121

Query: 134 IAHQSYIN--TVFGXSTDKKLALLLQNVQWISMDSLKQPHKEAEINQNKYQPILYHSSSY 193
           +   S +   T +  S D + A ++  V  + +DS +Q    +            H+ + 
Sbjct: 122 LTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSR----------QHTGAA 181

Query: 194 YGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPNDV----IVSWLPQ 253
           Y         +QYTSG+T  P  V+++      NV  +   Y  +P  +    +VSWLP 
Sbjct: 182 Y---------LQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPL 241

Query: 254 YHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKR 313
           YHD GL+  +   ++     +L SP+SF+ RP  W+ L+       +  P+F   L ++R
Sbjct: 242 YHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRR 301

Query: 314 VKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTF 373
             ++   + GLDLR +  ++  +E I+ + V  F+E F    L P  + PSYGLAE   +
Sbjct: 302 TSDQD--MAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLY 361

Query: 374 VSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCREI-------------EVVVVNGETG 433
           V+    G   +        Y +L        G    +              V +VN ET 
Sbjct: 362 VAAPEAGAAPK---TVRFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETM 421

Query: 434 EVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINN--KSSPN--FVRTGDRGVIKG 493
                GV GEIWV       GY   P  T + F +K+ +   ++P   ++RTGD GVI  
Sbjct: 422 VENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVI-- 481

Query: 494 ADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIA--LVAE 553
           +D  LF++GR  D++  +    +P  IE+         + GG  AA+ + D I   LVA 
Sbjct: 482 SDGELFIMGRIKDLLIVDGRNHYPDDIEATIQE-----ITGGRAAAIAVPDDITEQLVAI 541

Query: 554 MQRDDKNDAG-----LLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAV 591
           ++   +          LR +  E+  A+     + +  +VLV  G++P TTSGK++R A 
Sbjct: 542 IEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGKIRRSAC 576

BLAST of CcUC08G159330 vs. ExPASy Swiss-Prot
Match: B2HIL4 (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD29 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 6.9e-44
Identity = 159/570 (27.89%), Postives = 256/570 (44.91%), Query Frame = 0

Query: 52  LTYRQLHDSVQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 111
           LT+ Q++   ++++++L       D V VL   GLE +    G   AGL++VP+  P   
Sbjct: 79  LTWWQIYRRAKIVAEELRGYGASGDRVAVLAPQGLEYIIAFLGVLEAGLIAVPLPVPQFG 138

Query: 112 LENENCQHLARALSQTKPRAAIAHQSYINTVFGXSTDKKLAL----LLQNVQWISMDSLK 171
           + +E    ++ AL  + P   +     I+ V   +   +       ++  V  + +DS  
Sbjct: 139 IHDE---RISAALQDSTPSVILTTSPVIDEVTKYAPHARAGQGGTPIVVAVDLLDLDSA- 198

Query: 172 QPHKEAEINQNKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRA 231
              +E ++          HSS+ Y         +QYTSG+T  P  VV++      N   
Sbjct: 199 ---RELDLTPPA------HSSTAY---------LQYTSGSTRSPAGVVLSHKNVITNCVQ 258

Query: 232 ARKAYDLNPNDV---IVSWLPQYHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHL 291
               Y      V    VSWLP YHD GLM  ++L +I   T VL +P++F+ RP  W+ L
Sbjct: 259 LMSDYLGETEKVPSTAVSWLPFYHDMGLMLGIILPMINQDTAVLLNPMAFLQRPARWMQL 318

Query: 292 ITAFKATCTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVF 351
           +  F+   +  P+F   L ++R  +E   + GLDL  +R +    E +  + ++ F++ F
Sbjct: 319 MGKFRGQISCAPNFGFELAVRRTSDED--MAGLDLGHVRGIGSGAERVNPATLQRFIDRF 378

Query: 352 KAVGLDPGCVSPSYGLAENCTFVSTAWCGGDRR---WWFPTMPSYRKLLPSARLKDG--- 411
               L    + PSYGLAE   FV+TA  G   R   + + ++   R    +    DG   
Sbjct: 379 APFNLRDTAIRPSYGLAEATVFVATAEPGRPPRSVNFDYQSLSVGRVERCANEADDGAKL 438

Query: 412 ------WCREIEVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINN 471
                 W  E+   +V+ E       G  GEIWV   + A GY  +P +T  TF +K+ +
Sbjct: 439 VSYGSSWTSEVR--IVDPEARTECPAGTVGEIWVQGDNVAMGYWRNPQLTERTFDAKLTD 498

Query: 472 KSSPN----FVRTGDRGVIKGADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYL 531
            S       ++RTGD GV+   +  LF+ GR  D++  +    +P  IES         +
Sbjct: 499 PSPGTSIGPWLRTGDLGVMFEGE--LFITGRIKDLLIVDGSNHYPDDIESTIQE-----I 558

Query: 532 RGGCIAAVKISD-------TIALVAEMQRDDKNDAGLLRKICEEIRKALLIEEGIELGVV 591
            GG + A+ + D       TI   A      +     LR +  EI  A+     + +  V
Sbjct: 559 TGGRVVAIAVPDADGEKLVTIVEFASWGHSGQEAIDKLRSVKREITSAISRAHRVRVADV 615

BLAST of CcUC08G159330 vs. ExPASy TrEMBL
Match: A0A0A0LL23 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 4.3e-299
Identity = 524/629 (83.31%), Postives = 562/629 (89.35%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           MLYENFDPLFP+QPVVDRYLPVWASLPAF+SKPAFIWSEDGTA A+NE SFLTYRQLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQLI++QLLRQLRRRDTV+VLCSAGL+ VQLIYGCQRAGLVSVPISPPD F ENENC HL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 ARALSQTKPRAAIAHQSYINTVF---GXSTDKKLALLLQNVQWISMDSLKQPHKEAEINQ 180
           ARALSQTKPRAAIAHQSYINTVF     S DKKLALLL+ V+WISM+SLKQPHKE+E+NQ
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSSADKKLALLLKGVRWISMESLKQPHKESELNQ 180

Query: 181 NKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPN 240
           +K+Q   YHSSSY GC P++ YLIQYTSGATAI K VVITAGAAAHNVRAARKAYDLNPN
Sbjct: 181 HKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNPN 240

Query: 241 DVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSF 300
           D+IVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVT+P TWLHLITAFKATCTPVPSF
Sbjct: 241 DLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSF 300

Query: 301 TLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSY 360
           TLPLVLKRV   S     LDLRS+RNLILINEPIYRS VEEFVEVFK VGLDPGCVSPSY
Sbjct: 301 TLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSPSY 360

Query: 361 GLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVNGETGEVVED 420
           GLAENCTFVSTAWCGG     FP MPSYR+LLPS RL+DG C+EIEVVVVN ETGEVVED
Sbjct: 361 GLAENCTFVSTAWCGGG---GFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVED 420

Query: 421 GVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKGADRFLFVIG 480
           GVEGEIWVSSPSNA GYL HPSVT ETFHSK+ NKSS NFVRTGDRGVIKG+DRFLFVIG
Sbjct: 421 GVEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGSDRFLFVIG 480

Query: 481 RCSDVIEFNNH--EIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEMQRDDKNDA 540
           RCSDVI+ NN+  +IHPHYIES +YNN SAYLRGGCIAAVK+S TIALVAEMQRDD++DA
Sbjct: 481 RCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEMQRDDRHDA 540

Query: 541 GLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDGGGFGVLMA 600
            LLRKICE+IRKA+LIEEGIELG+VVLVKRGNV KTTSGKVKRW VKEKL GGG GVLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 VKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
            +F KNC    D +RK EF TRP+L S+L
Sbjct: 601 FEFGKNC---VDLKRKGEFETRPVLISML 623

BLAST of CcUC08G159330 vs. ExPASy TrEMBL
Match: A0A6J1HF51 (uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC111462961 PE=4 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.1e-291
Identity = 515/637 (80.85%), Postives = 552/637 (86.66%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD LNEGSFLTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQ ISD+L+RQ+RRRDTV++LCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC HL
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHHL 133

Query: 121 ARALSQTKPRAAIAHQSYINTVFG----XSTDKKLALLLQNVQWISMDSLK-----QPHK 180
           ARALSQTKPRAAIAHQ+YI +VF       TD KLALLLQ+VQWISM++L+     +  +
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEADQ 193

Query: 181 EAEINQNKYQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARK 240
              +  +K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVRAARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 253

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDP 360
           CTPVPSFTLPLVLKRVKEE+P   GLDL S+RNLILINEP+YRS VEEFV+VFKAVGL+P
Sbjct: 314 CTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLNP 373

Query: 361 GCVSPSYGLAENCTFVSTAWCGGD--RRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVN 420
           GCVSPSYGLAENCTFVSTAW GG+  RR WFP MPSYRKLLPSARL D    EIEVVVVN
Sbjct: 374 GCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVVN 433

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKG 480
           GETGE+VEDGVEGEIW+SSPSNASGYL HPS+TR+TFH K++NKSS  FVRTGDRGVIKG
Sbjct: 434 GETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 493

Query: 481 ADRFLFVIGRCSDVIEF-NNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEM 540
            DRFLFVIGRCSDVI   NN EIHPHYIESIAYNN S+YLRGGC+AA+KISDTIA+VAEM
Sbjct: 494 TDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVAEM 553

Query: 541 QRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDG 600
           QR DKNDA  LRKICE IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVKRWA KEKL G
Sbjct: 554 QRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKLAG 613

Query: 601 GGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
           GG  VLMAVKF K C V KD E       RPLL SLL
Sbjct: 614 GGMSVLMAVKFGKTCGVTKDLE-----TNRPLLLSLL 645

BLAST of CcUC08G159330 vs. ExPASy TrEMBL
Match: A0A6J1K7R6 (uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429 PE=4 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 1.1e-281
Identity = 502/637 (78.81%), Postives = 542/637 (85.09%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADALNEGSFLTYRQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD LNEGSFLTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISDQLLRQLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 120
           VQ IS+QL+RQ+RRRDTVIVLCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC H 
Sbjct: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120

Query: 121 ARALSQTKPRAAIAHQSYINTVF----GXSTDKKLALLLQNVQWISMDSLK-----QPHK 180
           ARALSQTKPRA IAH+ YI +VF        D KLALLLQ+VQWISM++L+     +  +
Sbjct: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180

Query: 181 EAEINQNKYQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARK 240
              +  +K +P  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVRAARK
Sbjct: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FKAT
Sbjct: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDP 360
           CTPVPSFTLPLVLKRVKEE+P    LDL  +RNLILINEP+YRS VEEFV+VFKAVGL+P
Sbjct: 301 CTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360

Query: 361 GCVSPSYGLAENCTFVSTAWCGGD--RRWWFPTMPSYRKLLPSARLKDGWCREIEVVVVN 420
           GCVSPSYGLAEN TFVSTAW GG+   R  FP MPSYRKLLP ARL DG   EIEVVVVN
Sbjct: 361 GCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVVN 420

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKG 480
           GETGE+V+DGVEGEIW+SSPSNASGYL HP +TR+TFH K++NKSS  FVRTGDRGVIKG
Sbjct: 421 GETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 480

Query: 481 ADRFLFVIGRCSDVIEF-NNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEM 540
           ADRFLFVIGRCSDVI   NN EIHPHYIESIAYNN SAYLRGGC+AA+K+SDTIA+VAEM
Sbjct: 481 ADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVAEM 540

Query: 541 QRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLDG 600
           QR+DKNDA  LR+ICE IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVKRW  KEKL G
Sbjct: 541 QREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKLAG 600

Query: 601 GGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
           GG  VLMAVKF   C V KD +       RPLL SLL
Sbjct: 601 GGMSVLMAVKFGTICGVTKDLD-----GNRPLLLSLL 632

BLAST of CcUC08G159330 vs. ExPASy TrEMBL
Match: A0A5A7VDG2 (Long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005700 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 6.8e-220
Identity = 394/481 (81.91%), Postives = 428/481 (88.98%), Query Frame = 0

Query: 148 DKKLALLLQNVQWISMDSLKQPHKEAEINQNKYQPILYHSSSYYGCKPEETYLIQYTSGA 207
           D+KLALLL++VQWISM+SLK+PHKE+E+NQ+K+    +HSSSY GC P++ YLIQYTSGA
Sbjct: 21  DEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGA 80

Query: 208 TAIPKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVL 267
           TAI KPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQ+HDCGLMFLLLTVITGATCVL
Sbjct: 81  TAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVL 140

Query: 268 TSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKEESPLIRG-LDLRSMRNLIL 327
           TSPISFVT+P TWLHLITAFKATCTPVPSFTLPLVLKRV   S    G LDLRS+RNLIL
Sbjct: 141 TSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLIL 200

Query: 328 INEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGDRRWWFPTMPSYR 387
           INEPIYRS VEEFV+VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGG     FP MPSYR
Sbjct: 201 INEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGG---GFPAMPSYR 260

Query: 388 KLLPSARLKDGWCREIEVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFH 447
           KLLPS RL+DG C+EIEVVVVN ETGEVVEDGVEGEIW+SSPSNA GYL HPS+T ETFH
Sbjct: 261 KLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFH 320

Query: 448 SKINNKSSPNFVRTGDRGVIKGADRFLFVIGRCSDVIEF--NNHEIHPHYIESIAYNNSS 507
            K+ NK+S NFVRTGDRGVIKG+DRFLFVIGRCSDVI+F  NN +IHPHYIES +Y N S
Sbjct: 321 RKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFS 380

Query: 508 AYLRGGCIAAVKISD-TIALVAEMQRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLV 567
           AYLRGGCIAAVKIS  T+ALVAEMQRDD+NDA LLRKICEEIRKA+LIEEGIELGVVVLV
Sbjct: 381 AYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLV 440

Query: 568 KRGNVPKTTSGKVKRWAVKEKLDGGGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSL 625
           KRGNV KTTSGKVKRW VKEKL GGG  VLMAV+F K+C+ LKD ERK EF TRP+L S+
Sbjct: 441 KRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSM 498

BLAST of CcUC08G159330 vs. ExPASy TrEMBL
Match: A0A1S3BAI6 (long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo OX=3656 GN=LOC103487973 PE=4 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 1.1e-212
Identity = 382/466 (81.97%), Postives = 413/466 (88.63%), Query Frame = 0

Query: 163 MDSLKQPHKEAEINQNKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAA 222
           M+SLK+PHKE+E+NQ+K+    +HSSSY GC P++ YLIQYTSGATAI KPVVITAGAAA
Sbjct: 1   MESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAA 60

Query: 223 HNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLH 282
           HNVRAARKAYDLNPNDVIVSWLPQ+HDCGLMFLLLTVITGATCVLTSPISFVT+P TWLH
Sbjct: 61  HNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLH 120

Query: 283 LITAFKATCTPVPSFTLPLVLKRVKEESPLIRG-LDLRSMRNLILINEPIYRSAVEEFVE 342
           LITAFKATCTPVPSFTLPLVLKRV   S    G LDLRS+RNLILINEPIYRS VEEFV+
Sbjct: 121 LITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVD 180

Query: 343 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCRE 402
           VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGG     FP MPSYRKLLPS RL+DG C+E
Sbjct: 181 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGG---GFPAMPSYRKLLPSGRLRDGMCKE 240

Query: 403 IEVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTG 462
           IEVVVVN ETGEVVEDGVEGEIW+SSPSNA GYL HPS+T ETFH K+ NK+S NFVRTG
Sbjct: 241 IEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTG 300

Query: 463 DRGVIKGADRFLFVIGRCSDVIEF--NNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISD 522
           DRGVIKG+DRFLFVIGRCSDVI+F  NN +IHPHYIES +Y N SAYLRGGCIAAVKIS 
Sbjct: 301 DRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISS 360

Query: 523 -TIALVAEMQRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKR 582
            T+ALVAEMQRDD+NDA LLRKICEEIRKA+LIEEGIELGVVVLVKRGNV KTTSGKVKR
Sbjct: 361 GTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKR 420

Query: 583 WAVKEKLDGGGFGVLMAVKFDKNCDVLKDSERKEEFRTRPLLPSLL 625
           W VKEKL GGG  VLMAV+F K+C+ LKD ERK EF TRP+L S+L
Sbjct: 421 WVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 463

BLAST of CcUC08G159330 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 87.0 bits (214), Expect = 5.7e-17
Identity = 102/405 (25.19%), Postives = 173/405 (42.72%), Query Frame = 0

Query: 195 PEETYLIQYTSGATAIPKPVVIT----AGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDC 254
           PE+T  + Y+SG T +PK V+IT      + A  V       +   NDVI+ +LP +H  
Sbjct: 209 PEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLPMFHIY 268

Query: 255 GLMFLLLTVI-TGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKEE 314
            L  L+L+ + TGA  ++             + LI  +K T  PV     P+VL  +K  
Sbjct: 269 ALDALMLSAMRTGAALLIVPRFEL----NLVMELIQRYKVTVVPVAP---PVVLAFIK-- 328

Query: 315 SPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTFVSTA 374
           SP     DL S+R ++L      +  +E+ V     +          YG+ E+ T   + 
Sbjct: 329 SPETERYDLSSVR-IMLSGAATLKKELEDAVR----LKFPNAIFGQGYGMTESGTVAKSL 388

Query: 375 WCGGDRRWWFPTMPSYRKLLPSARLKDGWC----REIEVVVVNGETGEVVEDGVEGEIWV 434
                    F   P         + K G C    R  E+ VV+ ETG  +     GEI V
Sbjct: 389 --------AFAKNP--------FKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICV 448

Query: 435 SSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKGADRFLFVIGRCSDVIEF 494
                  GYL+ P  T  T            ++ TGD G +   D  +F++ R  ++I+F
Sbjct: 449 RGHQLMKGYLNDPEATARTI-------DKDGWLHTGDIGFVDDDDE-IFIVDRLKELIKF 508

Query: 495 NNHEIHPHYIESIAYNNSSAYLRGGCIAAVK--ISD--TIALVAEMQRDDKNDAGLLRKI 554
             +++ P  +E++  ++ S  +    + A+K  ++D   +A VA  Q     +      +
Sbjct: 509 KGYQVAPAELEALLISHPS--IDDAAVVAMKDEVADEVPVAFVARSQGSQLTE----DDV 565

Query: 555 CEEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLD 587
              + K ++  + I++   + V    +PK  SGK+ R  ++ KL+
Sbjct: 569 KSYVNKQVVHYKRIKMVFFIEV----IPKAVSGKILRKDLRAKLE 565

BLAST of CcUC08G159330 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 80.1 bits (196), Expect = 6.9e-15
Identity = 137/563 (24.33%), Postives = 223/563 (39.61%), Query Frame = 0

Query: 49  GSFLTYRQLHDSVQLISDQLLR-QLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISP 108
           G   TY + H   + ++  L +  +R+ D +++L     E V    G    G VS   + 
Sbjct: 73  GKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVS---TT 132

Query: 109 PDPFLENENCQHLARALSQTKPRAAIAHQSYINTVFGXSTDKKLALLLQNVQWISMDSLK 168
            +PF  +   Q L + L  +  +  I H  Y++         KL  L +N+  I+ D   
Sbjct: 133 ANPFYTS---QELYKQLKSSGAKLIITHSQYVD---------KLKNLGENLTLITTD--- 192

Query: 169 QPHKE------AEINQNKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVIT---- 228
           +P  E        I  ++  P             ++   + ++SG T +PK VV+T    
Sbjct: 193 EPTPENCLPFSTLITDDETNPF----QETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSL 252

Query: 229 AGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVI-TGATCVLTSPISFVTR 288
             + A  V        L  NDVI+  LP +H   L  +LL  + +GAT +L         
Sbjct: 253 ITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEI--- 312

Query: 289 PTTWLHLITAFKATCTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAV 348
               L LI   + T   + +   PLV+   K  +P +   DL S+R ++    P+ +   
Sbjct: 313 -GALLDLIQRHRVT---IAALVPPLVIALAK--NPTVNSYDLSSVRFVLSGAAPLGKELQ 372

Query: 349 EEFVEVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKD 408
           +          L    +   YG+ E    +S +   G  +   PT             K 
Sbjct: 373 DSLRR-----RLPQAILGQGYGMTEAGPVLSMSL--GFAKEPIPT-------------KS 432

Query: 409 GWC----REIEVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNK 468
           G C    R  E+ VV+ ET   +     GEI +        YL+ P  T  T   +    
Sbjct: 433 GSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEE---- 492

Query: 469 SSPNFVRTGDRGVIKGADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYLRGGCI 528
               ++ TGD G +   D  +F++ R  +VI+F   ++ P  +ES+  N+ S        
Sbjct: 493 ---GWLHTGDIGYVDEDDE-IFIVDRLKEVIKFKGFQVPPAELESLLINHHSI----ADA 552

Query: 529 AAVKISDTIA---LVAEMQRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKR---- 586
           A V  +D +A    VA + R + ND      I EE  K  + ++      VV  KR    
Sbjct: 553 AVVPQNDEVAGEVPVAFVVRSNGND------ITEEDVKEYVAKQ------VVFYKRLHKV 560

BLAST of CcUC08G159330 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 70.1 bits (170), Expect = 7.2e-12
Identity = 122/561 (21.75%), Postives = 218/561 (38.86%), Query Frame = 0

Query: 49  GSFLTYRQLHDSVQLISDQLLR-QLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISP 108
           G   TY  +H + + ++  L    +++ D V++L     E+V         G ++   + 
Sbjct: 61  GEVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAIT---TS 120

Query: 109 PDPFLENENCQHLARALSQTKPRAAIAHQSYINTVFGXSTDKKLALLLQNVQWISMDSLK 168
            +PF         A+A   +  +  +    Y++ +     D         V  ++ DS  
Sbjct: 121 ANPFFTPAEISKQAKA---SAAKLIVTQSRYVDKIKNLQND--------GVLIVTTDSDA 180

Query: 169 QPH---KEAEINQNKYQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVIT----AGA 228
            P    + +E+ Q++   +    S      PE+   + ++SG T +PK V++T      +
Sbjct: 181 IPENCLRFSELTQSEEPRV---DSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTS 240

Query: 229 AAHNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTW 288
            A  V         N +DVI+  LP +H   L  ++L  +     +L  P   +   T  
Sbjct: 241 VAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEI---TLL 300

Query: 289 LHLITAFKATCTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFV 348
           L  I   K T   V     P+VL   K  SP     DL S+R +     P+ +   +   
Sbjct: 301 LEQIQRCKVT---VAMVVPPIVLAIAK--SPETEKYDLSSVRMVKSGAAPLGKELEDAIS 360

Query: 349 EVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWC- 408
             F    L  G     YG+ E    ++ +   G  +  FP             +K G C 
Sbjct: 361 AKFPNAKLGQG-----YGMTEAGPVLAMSL--GFAKEPFP-------------VKSGACG 420

Query: 409 ---REIEVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPN 468
              R  E+ +++ +TG+ +     GEI +       GYL+ P  T  T            
Sbjct: 421 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTI-------DKDG 480

Query: 469 FVRTGDRGVIKGADRFLFVIGRCSDVIEFNNHEIHPHYIESIAYNNSSAYLRGGCIAAVK 528
           ++ TGD G I   D  LF++ R  ++I++   ++ P  +ES+   +    +    + A+K
Sbjct: 481 WLHTGDVGFIDDDDE-LFIVDRLKELIKYKGFQVAPAELESLLIGHPE--INDVAVVAMK 540

Query: 529 ISDT--IALVAEMQRDDKNDAGLLRKICEEIRKALLIEEGIELGVVVLVKRGN------- 588
             D   + +   ++  D N       I E+  K  + ++      VV  KR N       
Sbjct: 541 EEDAGEVPVAFVVRSKDSN-------ISEDEIKQFVSKQ------VVFYKRINKVFFTDS 553

BLAST of CcUC08G159330 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 69.3 bits (168), Expect = 1.2e-11
Identity = 103/414 (24.88%), Postives = 175/414 (42.27%), Query Frame = 0

Query: 195 PEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPN------DVIVSWLPQYH 254
           P++   + Y+SG T +PK V++T      +V  A++    NPN      DVI+  LP +H
Sbjct: 201 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSV--AQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 255 DCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKE 314
              L  ++L  +     +L  P   +      L LI   K T  P+     P+VL   K 
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEI---NLLLELIQRCKVTVAPM---VPPIVLAIAK- 320

Query: 315 ESPLIRGLDLRSMRNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTFVST 374
            S      DL S+R +     P+ +   +     F    L  G     YG+ E    ++ 
Sbjct: 321 -SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQG-----YGMTEAGPVLAM 380

Query: 375 AWCGGDRRWWFPTMPSYRKLLPSARLKDGWC----REIEVVVVNGETGEVVEDGVEGEIW 434
           +   G  +  FP             +K G C    R  E+ +V+ +TG+ +     GEI 
Sbjct: 381 SL--GFAKEPFP-------------VKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEIC 440

Query: 435 VSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTGDRGVIKGADRFLFVIGRCSDVIE 494
           +       GYL++P+ T ET            ++ TGD G+I   D  LF++ R  ++I+
Sbjct: 441 IRGHQIMKGYLNNPAATAETI-------DKDGWLHTGDIGLIDDDDE-LFIVDRLKELIK 500

Query: 495 FNNHEIHPHYIESIAYNNSSAYLRGGCIAAVKISDTIALVAEMQRDDKNDAGLLRKICEE 554
           +   ++ P  +E++             I    I+D +A+VA M+ +   +  +   +  +
Sbjct: 501 YKGFQVAPAELEAL------------LIGHPDITD-VAVVA-MKEEAAGEVPVAFVV--K 560

Query: 555 IRKALLIEEGIELGV---VVLVKRGN-------VPKTTSGKVKRWAVKEKLDGG 589
            + + L E+ ++  V   VV  KR N       +PK  SGK+ R  ++ KL  G
Sbjct: 561 SKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLANG 560

BLAST of CcUC08G159330 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.2e-11
Identity = 108/457 (23.63%), Postives = 184/457 (40.26%), Query Frame = 0

Query: 49  GSFLTYRQLHDSVQLISDQLLR-QLRRRDTVIVLCSAGLELVQLIYGCQRAGLVSVPISP 108
           G  LT+ QL  +V  ++    R  +R+ D V++      +           G V    +P
Sbjct: 52  GDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANP 111

Query: 109 PDPFLENENCQHLARALSQTKPRAAIAHQSYINTVFGXSTDKKLALLLQNVQWISMDSLK 168
              +  NE    +++ +  + P+  I+    +N +F    DK     L  V   S D+++
Sbjct: 112 --LYTVNE----VSKQIKDSNPKIIIS----VNQLF----DKIKGFDLPVVLLGSKDTVE 171

Query: 169 QP--HKEAEINQNKYQPILYHSSSY--YGCKPEETYLIQYTSGATAIPKPVVITAGAAAH 228
            P       ++ +    +    S Y     K  +T  + Y+SG T   K V +T G    
Sbjct: 172 IPPGSNSKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIA 231

Query: 229 NVRAARKAYDL--NPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWL 288
                    DL    + V + +LP +H  G     L VIT +     + +  + R    L
Sbjct: 232 ASLMVTMDQDLMGEYHGVFLCFLPMFHVFG-----LAVITYSQLQRGNALVSMARFELEL 291

Query: 289 HLITAFKATCTPVPSFTLPLVLKRVKEESPLIRGLDLRSMRNLILINEPIYRSAVEEFVE 348
            L    K   T +  + +P V   + ++S +++  DL S++ +     P+ +  +EE   
Sbjct: 292 VLKNIEKFRVTHL--WVVPPVFLALSKQS-IVKKFDLSSLKYIGSGAAPLGKDLMEECGR 351

Query: 349 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGDRRWWFPTMPSYRKLLPSARLKDGWCRE 408
               V L  G     YG+ E C  VS      D R       S   L P           
Sbjct: 352 NIPNVLLMQG-----YGMTETCGIVSVE----DPRLGKRNSGSAGMLAPG---------- 411

Query: 409 IEVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLDHPSVTRETFHSKINNKSSPNFVRTG 468
           +E  +V+ ETG+      +GEIWV  P+   GYL++P  T+ET   K       ++V TG
Sbjct: 412 VEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK-------SWVHTG 459

Query: 469 DRGVIKGADRFLFVIGRCSDVIEFNNHEIHPHYIESI 499
           D G     D  L+V+ R  ++I++   ++ P  +E +
Sbjct: 472 DLGYF-NEDGNLYVVDRIKELIKYKGFQVAPAELEGL 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884878.10.0e+0086.35long-chain-fatty-acid--AMP ligase FadD26-like [Benincasa hispida][more]
XP_011649625.18.9e-29983.31uncharacterized protein LOC105434645 [Cucumis sativus] >KGN62605.1 hypothetical ... [more]
XP_022962563.12.4e-29180.85uncharacterized protein LOC111462961 [Cucurbita moschata][more]
XP_023546350.18.4e-28980.22uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo][more]
KAG7029389.11.4e-28880.06fadD26, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
B2HIN24.6e-4828.01Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium marinum (strain ATCC B... [more]
Q7TXM13.0e-4727.42Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium bovis (strain ATCC BAA... [more]
P9WQ426.7e-4727.26Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain C... [more]
P9WQ436.7e-4727.26Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain A... [more]
B2HIL46.9e-4427.894-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain AT... [more]
Match NameE-valueIdentityDescription
A0A0A0LL234.3e-29983.31AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690... [more]
A0A6J1HF511.1e-29180.85uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC1114629... [more]
A0A6J1K7R61.1e-28178.81uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429... [more]
A0A5A7VDG26.8e-22081.91Long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3BAI61.1e-21281.97long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT3G21230.15.7e-1725.194-coumarate:CoA ligase 5 [more]
AT1G65060.16.9e-1524.334-coumarate:CoA ligase 3 [more]
AT3G21240.17.2e-1221.754-coumarate:CoA ligase 2 [more]
AT1G51680.11.2e-1124.884-coumarate:CoA ligase 1 [more]
AT4G05160.11.2e-1123.63AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 32..484
e-value: 3.2E-70
score: 236.9
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 9..479
e-value: 1.8E-93
score: 315.8
NoneNo IPR availableGENE3D3.30.300.30coord: 481..599
e-value: 2.7E-18
score: 68.5
NoneNo IPR availablePANTHERPTHR22754:SF3510A19I.5coord: 1..604
NoneNo IPR availablePANTHERPTHR22754DISCO-INTERACTING PROTEIN 2 DIP2 -RELATEDcoord: 1..604
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 28..587
IPR040097Fatty acyl-AMP ligase /fatty acyl-coenzyme A (CoA) ligaseCDDcd05931FAALcoord: 24..585
e-value: 1.21932E-164
score: 479.812

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC08G159330.1CcUC08G159330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
molecular_function GO:0016874 ligase activity